CmoCh20G009170 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G009170
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionmalonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like
LocationCmo_Chr20: 4717650 .. 4719044 (-)
RNA-Seq ExpressionCmoCh20G009170
SyntenyCmoCh20G009170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCTACCAAAGAAGCCATGGTTGGAGATATTGGAGCAATGCAAAATTGGTCCCTCACCTTCCCCGCCCACACCCTTCTCTCTGCCTCTCACTTACTTCGATCTCAGTTTCTTCTTAAGCCCTCCCACTCAGCGCATTCTCTTCTACTCCTTACCTCCCCATGAGCTGCTTCACTTCTACTCCATACTCCAAACCCTCAAACACTCTCTCTCCCACGCCCTCTCCCACTTTCTCCCCCTCGCCGGAAACCTCGTTTGGCCGCCTCAATCCCCGGAGCCCTTTATTTGTTACAACCCTGGCGACTCTCTTTCCCTCACTATTGCCAAAACCCACGCTGATTTCCACCACCTCTCTTCAAATCATGCCCGGAAGGCAAAGGAATCCCATTTCCTCGTACCCGACCTCCCAACATCCGACACCATTGCTCCAGTCATGTCTCTTCAAATCACTTTGTTCCCCAAATGTGGGTTTTCCATTGGCATCATAACCAACCATGTGGTTTCCGATGCCAAAACATCCACCATGTTCTTGAAATCATGGGCTTCCATTTGTAGTACACTCAATAATACTAATAATAAGAATCCCCCCACGTTGCCGTCTGAGTTGACACCATGTTTTGATAGAACACCCGCCACGGATCCAAATGGGTTGCATACACTTTATGTTAAGTCTTTTGCAATCTTTGTCCCTAACCCTAAATTACTTGGACTTACCCCAAAAGAGGTCATCTCGGATGATGTGGTGTATGCCACGTTTGAGCTTACACGCATTGACATAGAGAAGTTGAGGAGAAGAGTGGTAGCAACTTCTTCATCCACTCCTCGTCGGTTATCAACTTTTATGTTGGCATTTTCTCTTGTTTCGACTTGCATCGTCAAAGCGCAACGAACCGAACCCGAAAGTAAGATAGGGTTGATCTTTCTTGTGGATTGGCGGGCTCGTATGGATGTGCTAGGGTGGCTCAATTATTTTGGTAATGGTGTAAATGGGTATGGATTGTTTGTTGAAGCGAGGGAATTAGAGGGAGAAAATGGGATGGCAATGATTTCAAACAAGATTAGTGATGCATTAGCGGAGATAGAGAAGAATATGAAGGAGAATAAAGGAGTGGAAATGTTGGATGAAATTTCAGAGAGATGGAAGAAAGCGATGCCCATAGATAAGTTTGTTTTAGTGGCGGGATCACCAAGGCTTGGTGTTTATGACATTGACTTTGGGTGGGGAAGGAGTAAGAAAGTGGAGCTTGCTTCCATAAGCCCTAATGGAGTTTTTTCAATGGCGGAGAGTAGAAATGGGGATGGGGGAGTTGAGCTTGGGATTTCTCTTCCAAGTGAAGCTGTGGACAAGTTTTGCTCTCTATTTTCAGAGACGATGAAAGGCTATGTTGATTAG

mRNA sequence

ATGGAGCTACCAAAGAAGCCATGGTTGGAGATATTGGAGCAATGCAAAATTGGTCCCTCACCTTCCCCGCCCACACCCTTCTCTCTGCCTCTCACTTACTTCGATCTCAGTTTCTTCTTAAGCCCTCCCACTCAGCGCATTCTCTTCTACTCCTTACCTCCCCATGAGCTGCTTCACTTCTACTCCATACTCCAAACCCTCAAACACTCTCTCTCCCACGCCCTCTCCCACTTTCTCCCCCTCGCCGGAAACCTCGTTTGGCCGCCTCAATCCCCGGAGCCCTTTATTTGTTACAACCCTGGCGACTCTCTTTCCCTCACTATTGCCAAAACCCACGCTGATTTCCACCACCTCTCTTCAAATCATGCCCGGAAGGCAAAGGAATCCCATTTCCTCGTACCCGACCTCCCAACATCCGACACCATTGCTCCAGTCATGTCTCTTCAAATCACTTTGTTCCCCAAATGTGGGTTTTCCATTGGCATCATAACCAACCATGTGGTTTCCGATGCCAAAACATCCACCATGTTCTTGAAATCATGGGCTTCCATTTGTAGTACACTCAATAATACTAATAATAAGAATCCCCCCACGTTGCCGTCTGAGTTGACACCATGTTTTGATAGAACACCCGCCACGGATCCAAATGGGTTGCATACACTTTATGTTAAGTCTTTTGCAATCTTTGTCCCTAACCCTAAATTACTTGGACTTACCCCAAAAGAGGTCATCTCGGATGATGTGGTGTATGCCACGTTTGAGCTTACACGCATTGACATAGAGAAGTTGAGGAGAAGAGTGGTAGCAACTTCTTCATCCACTCCTCGTCGGTTATCAACTTTTATGTTGGCATTTTCTCTTGTTTCGACTTGCATCGTCAAAGCGCAACGAACCGAACCCGAAAGTAAGATAGGGTTGATCTTTCTTGTGGATTGGCGGGCTCGTATGGATGTGCTAGGGTGGCTCAATTATTTTGGTAATGGTGTAAATGGGTATGGATTGTTTGTTGAAGCGAGGGAATTAGAGGGAGAAAATGGGATGGCAATGATTTCAAACAAGATTAGTGATGCATTAGCGGAGATAGAGAAGAATATGAAGGAGAATAAAGGAGTGGAAATGTTGGATGAAATTTCAGAGAGATGGAAGAAAGCGATGCCCATAGATAAGTTTGTTTTAGTGGCGGGATCACCAAGGCTTGGTGTTTATGACATTGACTTTGGGTGGGGAAGGAGTAAGAAAGTGGAGCTTGCTTCCATAAGCCCTAATGGAGTTTTTTCAATGGCGGAGAGTAGAAATGGGGATGGGGGAGTTGAGCTTGGGATTTCTCTTCCAAGTGAAGCTGTGGACAAGTTTTGCTCTCTATTTTCAGAGACGATGAAAGGCTATGTTGATTAG

Coding sequence (CDS)

ATGGAGCTACCAAAGAAGCCATGGTTGGAGATATTGGAGCAATGCAAAATTGGTCCCTCACCTTCCCCGCCCACACCCTTCTCTCTGCCTCTCACTTACTTCGATCTCAGTTTCTTCTTAAGCCCTCCCACTCAGCGCATTCTCTTCTACTCCTTACCTCCCCATGAGCTGCTTCACTTCTACTCCATACTCCAAACCCTCAAACACTCTCTCTCCCACGCCCTCTCCCACTTTCTCCCCCTCGCCGGAAACCTCGTTTGGCCGCCTCAATCCCCGGAGCCCTTTATTTGTTACAACCCTGGCGACTCTCTTTCCCTCACTATTGCCAAAACCCACGCTGATTTCCACCACCTCTCTTCAAATCATGCCCGGAAGGCAAAGGAATCCCATTTCCTCGTACCCGACCTCCCAACATCCGACACCATTGCTCCAGTCATGTCTCTTCAAATCACTTTGTTCCCCAAATGTGGGTTTTCCATTGGCATCATAACCAACCATGTGGTTTCCGATGCCAAAACATCCACCATGTTCTTGAAATCATGGGCTTCCATTTGTAGTACACTCAATAATACTAATAATAAGAATCCCCCCACGTTGCCGTCTGAGTTGACACCATGTTTTGATAGAACACCCGCCACGGATCCAAATGGGTTGCATACACTTTATGTTAAGTCTTTTGCAATCTTTGTCCCTAACCCTAAATTACTTGGACTTACCCCAAAAGAGGTCATCTCGGATGATGTGGTGTATGCCACGTTTGAGCTTACACGCATTGACATAGAGAAGTTGAGGAGAAGAGTGGTAGCAACTTCTTCATCCACTCCTCGTCGGTTATCAACTTTTATGTTGGCATTTTCTCTTGTTTCGACTTGCATCGTCAAAGCGCAACGAACCGAACCCGAAAGTAAGATAGGGTTGATCTTTCTTGTGGATTGGCGGGCTCGTATGGATGTGCTAGGGTGGCTCAATTATTTTGGTAATGGTGTAAATGGGTATGGATTGTTTGTTGAAGCGAGGGAATTAGAGGGAGAAAATGGGATGGCAATGATTTCAAACAAGATTAGTGATGCATTAGCGGAGATAGAGAAGAATATGAAGGAGAATAAAGGAGTGGAAATGTTGGATGAAATTTCAGAGAGATGGAAGAAAGCGATGCCCATAGATAAGTTTGTTTTAGTGGCGGGATCACCAAGGCTTGGTGTTTATGACATTGACTTTGGGTGGGGAAGGAGTAAGAAAGTGGAGCTTGCTTCCATAAGCCCTAATGGAGTTTTTTCAATGGCGGAGAGTAGAAATGGGGATGGGGGAGTTGAGCTTGGGATTTCTCTTCCAAGTGAAGCTGTGGACAAGTTTTGCTCTCTATTTTCAGAGACGATGAAAGGCTATGTTGATTAG

Protein sequence

MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTPKEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEPESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAEIEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASISPNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD
Homology
BLAST of CmoCh20G009170 vs. ExPASy Swiss-Prot
Match: Q9LJB4 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase OS=Arabidopsis thaliana OX=3702 GN=5MAT PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 3.3e-75
Identity = 176/452 (38.94%), Postives = 251/452 (55.53%), Query Frame = 0

Query: 9   LEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYSILQTLK 68
           + ILE  ++ P  S     +LPLTYFDL +    P  R+LFY +P    L   S++  LK
Sbjct: 8   VNILEVVQVSPPSS--NSLTLPLTYFDLGWLKLHPVDRVLFYHVPE---LTRSSLISKLK 67

Query: 69  HSLSHALSHFLPLAGNLVWPPQSPEPFICYNPG--DSLSLTIAKTHADFHHLSSNHARKA 128
            SLS  L H+LPLAG LVW     +P I Y+P   D++ LT+A+++ D  HLS +  R A
Sbjct: 68  SSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEPRPA 127

Query: 129 KESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICS 188
            E H LVP+LP SD  A V+++Q+T FP  GFS+G+  +H V D KT+ MFLK+WA  C 
Sbjct: 128 TEFHSLVPELPVSDESARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAWAHNC- 187

Query: 189 TLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTPKEVISD 248
                  +    LP +L P  DR    DP GL T  +  +     N   L L P ++I  
Sbjct: 188 ------KQEQEALPHDLVPSLDRIIVQDPTGLETKLLNRWISASNNKPSLKLFPSKIIGS 247

Query: 249 DVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEPESKIGL 308
           D++  T+ LTR DI+KLR RV   S +   RLSTF++ ++ V TC+VK +  +P   + +
Sbjct: 248 DILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVCV 307

Query: 309 IFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARE--LEGENGMAMISNKISDALAEIEKN 368
            F  D+R+R++      +FGN + G G F    E  LE   G   I+  +      +   
Sbjct: 308 GFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFIT-AVETLTGWVNGL 367

Query: 369 MKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASISPNGV 428
             EN    ML    E +K+  P  + + VAGS RLG+Y  DFGWG+  KVE+ +I  +  
Sbjct: 368 CPENIEKNMLLPF-EAFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKDAS 427

Query: 429 FSMAESRNGDGGVELGISLPSEAVDKFCSLFS 457
            S++ES +G GGVE+G+ L  + V++F SLFS
Sbjct: 428 VSLSESGDGSGGVEVGVCLKKDDVERFGSLFS 445

BLAST of CmoCh20G009170 vs. ExPASy Swiss-Prot
Match: Q940Z5 (Phenolic glucoside malonyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PMAT1 PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 5.3e-73
Identity = 178/471 (37.79%), Postives = 256/471 (54.35%), Query Frame = 0

Query: 9   LEILEQCKIGPSPSPPT-PFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYS-ILQT 68
           L++++  ++ PS S  +   +LPLT+FDL ++     +R++FY L       F S I+  
Sbjct: 10  LKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIVPN 69

Query: 69  LKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARKA 128
           LK SLS +LSH+LPLAG LVW P  P+P I Y P D++S T+A+++ADF  L+       
Sbjct: 70  LKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPFPT 129

Query: 129 KESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICS 188
            E + LVP+L  SD  A  +S Q+TLFP  GF I +  +H V D KT+T FLKSWA  C 
Sbjct: 130 TELYPLVPELHVSDDSASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWARTCK 189

Query: 189 TLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSF----AIFV-----PNPKLLG 248
             ++        LP +L P +DRT   DP  L T  + ++     +F       NPK L 
Sbjct: 190 NQDS-------FLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKSLK 249

Query: 249 LTPKEVISDDVVYATFELTRIDIEKLRRRVVATS-----SSTPR--RLSTFMLAFSLVST 308
           L     I  DV   T  LTR DI+KLR R+   S     SS+P+  RLSTF++ +S   T
Sbjct: 250 LLWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALT 309

Query: 309 CIVKAQRTEPESKIGLIFLVDWRARMDVLGWLNYFGNGVNG-YGLFVEARELEGENGMAM 368
           C++KA+  +P   +G  F VD R+ M      +YFGN V+  + + + A     E G   
Sbjct: 310 CLIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLA 369

Query: 369 ISNKISDALAEIEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWG 428
            +  +SD++  +++N+        + EI E +    P  + + VAGS R GVY +DFGWG
Sbjct: 370 AARMVSDSVEALDENV-----ALKIPEILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWG 429

Query: 429 RSKKVELASISPNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMK 461
           R +KV + SI      S AESR+G GGVELG SL    +D    L  + ++
Sbjct: 430 RPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLE 468

BLAST of CmoCh20G009170 vs. ExPASy Swiss-Prot
Match: Q9LRQ8 (Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2 PE=1 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 3.2e-70
Identity = 173/457 (37.86%), Postives = 241/457 (52.74%), Query Frame = 0

Query: 9   LEILEQCKIGPSP----SPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYS-I 68
           L ++E  ++ P+     +      LPLT+FDL + L  P +R+ FY L      HF+S I
Sbjct: 3   LHVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHFHSII 62

Query: 69  LQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHA 128
           L  LK SLS  L ++LPL G++ W P  P+P I  +    + +TIA++ ADF HLS    
Sbjct: 63  LPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAESDADFSHLSGYGQ 122

Query: 129 RKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWAS 188
           R   E H LVP LP SD  A   S+QITLFP  GFSIG+  +H V D KTS+ F+K+WA 
Sbjct: 123 RPLSELHALVPKLPVSDDSATAFSIQITLFPNQGFSIGVAAHHAVLDGKTSSTFIKAWAQ 182

Query: 189 ICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTL---YVKSFAIFVPNPKLLGLTP 248
           IC        +   ++P  LTP +DR+    P  L       V+S      N + L   P
Sbjct: 183 IC-------KQELQSMPENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLTSLP 242

Query: 249 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 308
              + DDVV AT  L+R DIE+LR +V   S S    LSTF++A++   TC VKA+    
Sbjct: 243 SSKLGDDVVLATLVLSRADIERLREQVKNVSPSL--HLSTFVIAYAYAWTCFVKARGGNK 302

Query: 309 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLF-VEARELEGENGMAMISNKISDALA 368
           +  + L+F+ D+R R+D      YFGN +   G +  +A E   E G    +  ISD   
Sbjct: 303 DRSVSLLFVGDFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAAEIISD--- 362

Query: 369 EIEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASI 428
            + K +   K   + D   E +       +F  +AGS RLGVY+ DFGWGR  KV++ SI
Sbjct: 363 -LVKGLSSRKIETIADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVDIVSI 422

Query: 429 SPNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFS 457
                 +MAE R+  GGVE+G+ L    +D   S F+
Sbjct: 423 DQGEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFN 446

BLAST of CmoCh20G009170 vs. ExPASy Swiss-Prot
Match: Q9LRQ7 (BAHD acyltransferase At3g29680 OS=Arabidopsis thaliana OX=3702 GN=At3g29680 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 3.0e-68
Identity = 172/465 (36.99%), Postives = 252/465 (54.19%), Query Frame = 0

Query: 9   LEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFY-SILQTL 68
           L +++  ++    +   P  LPLT+FDL +    P +R+ FY L       F+ SIL  L
Sbjct: 3   LNVIKISRVSLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSFFSSILPKL 62

Query: 69  KHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARKAK 128
           + SLS  LSHFLPL+G+L W PQ P+P I   P D++SLT+ ++ ADF ++SS   R   
Sbjct: 63  EQSLSLVLSHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEADFSYISSKELRLET 122

Query: 129 ESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICST 188
           E   LVP+L  S   A ++SLQITLFP  GFSIG   +HVV D KT++ F KSWA IC  
Sbjct: 123 ELRPLVPELQVSSDSASLLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWAHICKH 182

Query: 189 LNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPK----LLGLTPKEV 248
                + + PT+        DRT    P GL    +   + ++   K     L L P + 
Sbjct: 183 GTTPQDFDLPTV-------LDRTVINVPAGLEQ-KIFQLSSYISEEKDYARTLTLPPAKE 242

Query: 249 ISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEPESK 308
           I +DVV  T ELT +DIEKL+ R    S+ +   LSTF+++++ V TC+VK+   +    
Sbjct: 243 IDNDVVRVTLELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGDANRP 302

Query: 309 IGLIFLVDWRARMDVLGWLNYFGNGV-----NGYGLFVEARELEGENGMAMISNKISDAL 368
           +  ++  D+R R+D    L YFGN V     NGY    +A    G++G       +SD++
Sbjct: 303 VRFMYAADFRNRLDPPVPLTYFGNCVLPIDFNGY----KATTFLGKDGYVNGVEILSDSV 362

Query: 369 AEIEKNMKENKGVEMLDEISERWKKAMPID-KFVLVAGSPRLGVYDIDFGWGRSKKVELA 428
             +      ++ +E + E+ E   K M +D + V V GS + G+Y  DFGWGR  K ++ 
Sbjct: 363 RGL-----GSRNIESIWEVYEDGTKNMKLDTQNVTVTGSNQFGIYGSDFGWGRPVKTDVM 422

Query: 429 SISPNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGY 463
           S+  N  FSM+  R+  GG+E+GISL    ++ F SLF+     Y
Sbjct: 423 SLYKNNEFSMSARRDEIGGLEIGISLKKCEMNVFLSLFTSDFDIY 450

BLAST of CmoCh20G009170 vs. ExPASy Swiss-Prot
Match: Q9FNP9 (Agmatine coumaroyltransferase OS=Arabidopsis thaliana OX=3702 GN=ACT PE=1 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.3e-63
Identity = 159/458 (34.72%), Postives = 243/458 (53.06%), Query Frame = 0

Query: 9   LEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHEL---LHFYSILQ 68
           L++++  ++ P+ +   P  +PL++FDL +    PT+++ FY L        + + SIL 
Sbjct: 3   LKVIKISRVSPATASVDPLIVPLSFFDLQWLKLNPTEQVFFYKLTESSSSRDVFYSSILP 62

Query: 69  TLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARK 128
            L+ SLS  L+HF    G+L W  Q P+P +    GD+LSLT+A+T ADF  +S    R 
Sbjct: 63  KLERSLSLILTHFRLFTGHLKWDSQDPKPHLVVLSGDTLSLTVAETDADFSRISGRGLRP 122

Query: 129 AKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASIC 188
             E   L+P+LP       V+SLQ+TLFPK GF IG   +HVV D KT+  F K+WA  C
Sbjct: 123 ELELRPLIPELPIYSDSGAVVSLQVTLFPKQGFCIGTTAHHVVLDGKTAEKFNKAWAHTC 182

Query: 189 STLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFV----PNPKLLGLTPK 248
                       T+P  L    DR+    P GL    ++           N + L L P 
Sbjct: 183 ---------KHGTIPKILPTVLDRSVVNVPAGLEQKMLELLPYLTEDDKENGRTLKLPPV 242

Query: 249 EVIS--DDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTE 308
           + I+  D+V+  T E++  +IEKL+ R    S+     LSTF++ F+ V TC+VKA+  +
Sbjct: 243 KEINAKDNVLRITIEISPENIEKLKERAKKESTRAELHLSTFVVTFAHVWTCMVKARSGD 302

Query: 309 PESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLF-VEARELEGENGMAMISNKISDAL 368
           P   +  ++  D+R R++    + YFG  V     +  +A+E  GE+G       +SD++
Sbjct: 303 PNRPVRFMYAADFRNRLEPPVPVTYFGTCVLAMDFYKYKAKEFMGEDGFVNTVEILSDSV 362

Query: 369 AEIEKNMKENKGVEMLDEISERWKKAMPI-DKFVLVAGSPRLGVYDIDFGWGRSKKVELA 428
             +      ++GVE   ++ E   K M    + ++V GS ++G+Y+ DFGWGR    E  
Sbjct: 363 KRL-----ASQGVESTWKVYEEGTKTMKWGTQLLVVNGSNQIGMYETDFGWGRPIHTETM 422

Query: 429 SISPNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLF 456
           SI  N  FSM++ R+G GGVE+GISL    +D F SLF
Sbjct: 423 SIYKNDEFSMSKRRDGIGGVEIGISLKKLEMDTFLSLF 446

BLAST of CmoCh20G009170 vs. ExPASy TrEMBL
Match: A0A6J1FVL4 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448866 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.3e-268
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 60
           MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF
Sbjct: 6   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 65

Query: 61  YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 120
           YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS
Sbjct: 66  YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 125

Query: 121 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 180
           NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS
Sbjct: 126 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 185

Query: 181 WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 240
           WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP
Sbjct: 186 WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 245

Query: 241 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 300
           KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP
Sbjct: 246 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 305

Query: 301 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE 360
           ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE
Sbjct: 306 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE 365

Query: 361 IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 420
           IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS
Sbjct: 366 IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 425

Query: 421 PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 465
           PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD
Sbjct: 426 PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 469

BLAST of CmoCh20G009170 vs. ExPASy TrEMBL
Match: A0A6J1JEA3 (phenolic glucoside malonyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484235 PE=3 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 6.6e-228
Identity = 402/464 (86.64%), Postives = 423/464 (91.16%), Query Frame = 0

Query: 1   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 60
           MELPKKPWLEILEQCKIGPSPSPPTPFSLPLT FDLSFF +PPTQ ILFYSL PH+LLH 
Sbjct: 6   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTLFDLSFFSAPPTQHILFYSLSPHQLLHL 65

Query: 61  YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 120
            SIL  LKHSLSHALSHFLPLAG+LVWPPQSP+PFI YNPGDS+SLTIAKTHADFH LSS
Sbjct: 66  DSILLNLKHSLSHALSHFLPLAGSLVWPPQSPDPFILYNPGDSVSLTIAKTHADFHLLSS 125

Query: 121 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 180
           NHARKA ESHFLVP LPTSDTIAP MSLQITLFPK GF IGIITNHVVSDAKTSTMFLKS
Sbjct: 126 NHARKATESHFLVPQLPTSDTIAPAMSLQITLFPKSGFCIGIITNHVVSDAKTSTMFLKS 185

Query: 181 WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 240
           WASICSTLNNTNNKNPPTLPSELTPCFDRT ATDPNGLHT+YVKSF IFV  PKLLGL P
Sbjct: 186 WASICSTLNNTNNKNPPTLPSELTPCFDRTSATDPNGLHTIYVKSFEIFV--PKLLGLAP 245

Query: 241 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 300
           KEVISDDVVYATFELT IDIEK+RRRVVATSSSTPRRL+T MLAFSL STCIVKAQR  P
Sbjct: 246 KEVISDDVVYATFELTCIDIEKVRRRVVATSSSTPRRLTTLMLAFSLASTCIVKAQRIAP 305

Query: 301 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE 360
           E KIGLIFLVDWRARMD+LG LNYFGN V+ YG+F EARELE ENGMAMISNKIS+ + E
Sbjct: 306 ECKIGLIFLVDWRARMDMLGGLNYFGNCVSAYGVFAEARELEEENGMAMISNKISEEIEE 365

Query: 361 IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 420
           IEKN KENK VEML+ ISERW+K MPIDK ++VAGSPRLGVYDIDFGWGRSKKVE  SIS
Sbjct: 366 IEKNGKENKIVEMLEAISERWRKEMPIDKLIIVAGSPRLGVYDIDFGWGRSKKVEQVSIS 425

Query: 421 PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 465
           PNGVFSMAESRNGDGGVELGI+LP +A+DKFCSLFSET+KGYVD
Sbjct: 426 PNGVFSMAESRNGDGGVELGIALPPQAMDKFCSLFSETVKGYVD 467

BLAST of CmoCh20G009170 vs. ExPASy TrEMBL
Match: A0A6J1JFR0 (phenolic glucoside malonyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483994 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.1e-220
Identity = 390/456 (85.53%), Postives = 412/456 (90.35%), Query Frame = 0

Query: 9   LEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYSILQTLK 68
           +EILE CKI PSPSPPTPFSLPLT FDLSFF +PPTQ ILFYSL PH+LLH  SIL  LK
Sbjct: 3   MEILEVCKISPSPSPPTPFSLPLTLFDLSFFSAPPTQHILFYSLSPHQLLHLDSILLNLK 62

Query: 69  HSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARKAKE 128
           HSLSHALSHFLPLAG+LVWPPQSP+PFI YNPGDS+SLTIAKTHADFH LSSNHARKA E
Sbjct: 63  HSLSHALSHFLPLAGSLVWPPQSPDPFILYNPGDSVSLTIAKTHADFHLLSSNHARKATE 122

Query: 129 SHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICSTL 188
           SHFLVP LPTSDTIAP MSLQITLFPK GF IGIITNHVVSDAKTSTMFLKSWASICSTL
Sbjct: 123 SHFLVPQLPTSDTIAPAMSLQITLFPKSGFCIGIITNHVVSDAKTSTMFLKSWASICSTL 182

Query: 189 NNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTPKEVISDDV 248
           NNTNNKNPPTLPSELTPCFDRT ATDPNGLHT+YVKSF IFV  PKLLGL PKEVISDDV
Sbjct: 183 NNTNNKNPPTLPSELTPCFDRTSATDPNGLHTIYVKSFEIFV--PKLLGLAPKEVISDDV 242

Query: 249 VYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEPESKIGLIF 308
           VYATFELT IDIEK+RRRVVATSSSTPRRL+T MLAFSL STCIVKAQR  PE KIGLIF
Sbjct: 243 VYATFELTCIDIEKVRRRVVATSSSTPRRLTTLMLAFSLASTCIVKAQRIAPECKIGLIF 302

Query: 309 LVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAEIEKNMKEN 368
           LVDWRARMD+LG LNYFGN V+ YG+F EARELE ENGMAMISNKIS+ + EIEKN KEN
Sbjct: 303 LVDWRARMDMLGGLNYFGNCVSAYGVFAEARELEEENGMAMISNKISEEIEEIEKNGKEN 362

Query: 369 KGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASISPNGVFSMA 428
           K VEML+ ISERW+K MPIDK ++VAGSPRLGVYDIDFGWGRSKKVE  SISPNGVFSMA
Sbjct: 363 KIVEMLEAISERWRKEMPIDKLIIVAGSPRLGVYDIDFGWGRSKKVEQVSISPNGVFSMA 422

Query: 429 ESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 465
           ESRNGDGGVELGI+LP +A+DKFCSLFSET+K YVD
Sbjct: 423 ESRNGDGGVELGIALPPQAMDKFCSLFSETVKSYVD 456

BLAST of CmoCh20G009170 vs. ExPASy TrEMBL
Match: A0A6J1FXM3 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448598 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 7.7e-184
Identity = 333/456 (73.03%), Postives = 378/456 (82.89%), Query Frame = 0

Query: 5   KKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYSIL 64
           +K  +E+LE C I PSPSPPT FSLPLT+FDL  FL+PPT+RILFY+LP ++L  F SIL
Sbjct: 18  QKAIMEVLEVCNITPSPSPPTHFSLPLTFFDLVQFLAPPTERILFYTLPANDLPRFDSIL 77

Query: 65  QTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHAR 124
           QTLKHSLS  LSHFLPLAGNLVWP  S +PFI YNPGDS+SLTIAKT ADFHHLSSNH R
Sbjct: 78  QTLKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTEADFHHLSSNHVR 137

Query: 125 KAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASI 184
           KAKESHFLVP LPTSDTIAP MSLQITLFPK GFSIGI+TNHVV D KTSTMFLKSWASI
Sbjct: 138 KAKESHFLVPQLPTSDTIAPPMSLQITLFPKSGFSIGIVTNHVVVDGKTSTMFLKSWASI 197

Query: 185 CSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTPKEVI 244
           CSTLN TNN N PTLP ELTP FDRT A DPNGL  LYVK +  FV  PK+LGLTPK VI
Sbjct: 198 CSTLNCTNN-NLPTLPPELTPFFDRTAARDPNGLQRLYVKYYGAFVRQPKILGLTPKRVI 257

Query: 245 SDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEPESKI 304
           SDDVVYATFELTR DIEKLR +VVATSSSTPRRLSTFMLAFSL+STCIVKA+R EP+SKI
Sbjct: 258 SDDVVYATFELTRTDIEKLRTKVVATSSSTPRRLSTFMLAFSLISTCIVKAERIEPKSKI 317

Query: 305 GLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAEIEKN 364
            L F +D R RM  LG LNYFGN V+  G+F+EA+ELE ENGMAM++NKISD + EIE +
Sbjct: 318 ALFFAMDMRPRMGALGGLNYFGNCVSTLGVFLEAKELEEENGMAMVANKISDRMMEIENS 377

Query: 365 MKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASISPNGV 424
           +KENK VEM++EI  RW + MPI K + V GSPR GVY+IDFGWG SKKVE+AS+ PNG 
Sbjct: 378 VKENKVVEMVEEILGRWGREMPISKMIGVGGSPRFGVYEIDFGWGNSKKVEMASMDPNGT 437

Query: 425 FSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMK 461
           FS+ ESR+G+GGVE+GI+LP++ ++   SLF E +K
Sbjct: 438 FSITESRSGNGGVEVGIALPNDVMNVLSSLFYEEVK 472

BLAST of CmoCh20G009170 vs. ExPASy TrEMBL
Match: A0A6J1J6U6 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111483993 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 2.0e-179
Identity = 325/455 (71.43%), Postives = 372/455 (81.76%), Query Frame = 0

Query: 6   KPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYSILQ 65
           K  +E+LE C I PS SPPT FSLPLT+ DL  FLSPPT+RILFY+L  + L  F SILQ
Sbjct: 7   KAIMEVLEVCNITPSTSPPTHFSLPLTFSDLVQFLSPPTERILFYTLHANHLPRFDSILQ 66

Query: 66  TLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARK 125
           TLKHSLS  LSHFLPLAGNLVWP  SP+PFI YN GDS+SLTIAKT ADFHHLSSNH RK
Sbjct: 67  TLKHSLSLTLSHFLPLAGNLVWPHHSPDPFILYNHGDSVSLTIAKTEADFHHLSSNHVRK 126

Query: 126 AKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASIC 185
           A ESHFLVP LPTSDTIAP MSLQITLFPK GFSIGIITNHVV D KTSTMFLKSWASIC
Sbjct: 127 ATESHFLVPQLPTSDTIAPAMSLQITLFPKSGFSIGIITNHVVVDGKTSTMFLKSWASIC 186

Query: 186 STLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTPKEVIS 245
           STLN TNN N PTLPSELTP FDRT A DPNGL  LYVK +  FV  PK+LGLTPK VIS
Sbjct: 187 STLNCTNNNNLPTLPSELTPFFDRTAAKDPNGLQRLYVKHYGAFVRQPKILGLTPKRVIS 246

Query: 246 DDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEPESKIG 305
           DDVVYATFELTRIDIEKLRR+ VAT+S++ R L+TF+L ++LVSTCIV+A+R EP+SKI 
Sbjct: 247 DDVVYATFELTRIDIEKLRRK-VATNSTSTRHLTTFVLTYALVSTCIVQAERIEPKSKIA 306

Query: 306 LIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAEIEKNM 365
           L F VD R RM  LG LNYFGNGV G G  +EA+ELE ENGMAM++NKISD + E+EK++
Sbjct: 307 LFFAVDMRPRMGALGGLNYFGNGVIGLGEILEAKELEEENGMAMVANKISDRIMEMEKSV 366

Query: 366 KENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASISPNGVF 425
           KENK VEM++EIS+RW + MP+ K ++  GSPR GVY+IDFGWG SKKVE+AS+ PNG F
Sbjct: 367 KENKVVEMVEEISDRWGREMPVSKMIVAGGSPRFGVYEIDFGWGNSKKVEMASMDPNGSF 426

Query: 426 SMAESRNGDGGVELGISLPSEAVDKFCSLFSETMK 461
           SM ESR+G+GGVE+G+ LP + ++ F SLF E +K
Sbjct: 427 SMTESRSGNGGVEVGVVLPKDVMNVFSSLFYEEVK 460

BLAST of CmoCh20G009170 vs. NCBI nr
Match: XP_022944412.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like [Cucurbita moschata])

HSP 1 Score: 934.9 bits (2415), Expect = 2.7e-268
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 60
           MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF
Sbjct: 6   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 65

Query: 61  YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 120
           YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS
Sbjct: 66  YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 125

Query: 121 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 180
           NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS
Sbjct: 126 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 185

Query: 181 WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 240
           WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP
Sbjct: 186 WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 245

Query: 241 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 300
           KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP
Sbjct: 246 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 305

Query: 301 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE 360
           ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE
Sbjct: 306 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE 365

Query: 361 IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 420
           IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS
Sbjct: 366 IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 425

Query: 421 PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 465
           PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD
Sbjct: 426 PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 469

BLAST of CmoCh20G009170 vs. NCBI nr
Match: KAG6571175.1 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 911.4 bits (2354), Expect = 3.2e-261
Identity = 453/464 (97.63%), Postives = 460/464 (99.14%), Query Frame = 0

Query: 1   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 60
           MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF
Sbjct: 6   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 65

Query: 61  YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 120
            SILQTLKHSLSHALSHFLPLAGNL+WPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS
Sbjct: 66  DSILQTLKHSLSHALSHFLPLAGNLLWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 125

Query: 121 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 180
           NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS
Sbjct: 126 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 185

Query: 181 WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 240
           WASICSTLNNTNNKNPPTLPS+LTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP
Sbjct: 186 WASICSTLNNTNNKNPPTLPSQLTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 245

Query: 241 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 300
           KEVISDDVVYATFELT IDIEKLRRRVVATSSSTPRRLS+FMLAFSLVSTCIVKAQR EP
Sbjct: 246 KEVISDDVVYATFELTCIDIEKLRRRVVATSSSTPRRLSSFMLAFSLVSTCIVKAQRIEP 305

Query: 301 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE 360
           ESKIGLIFLVDWRARMDVLG LNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE
Sbjct: 306 ESKIGLIFLVDWRARMDVLGGLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE 365

Query: 361 IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 420
           IEKN+KENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS
Sbjct: 366 IEKNVKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 425

Query: 421 PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 465
           PNGVFSMAESRNGDGGVELGI+LPSEA+DKFCSLFSET+KGYVD
Sbjct: 426 PNGVFSMAESRNGDGGVELGIALPSEAMDKFCSLFSETVKGYVD 469

BLAST of CmoCh20G009170 vs. NCBI nr
Match: KAG7010980.1 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 907.9 bits (2345), Expect = 3.6e-260
Identity = 451/464 (97.20%), Postives = 458/464 (98.71%), Query Frame = 0

Query: 1   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 60
           MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF
Sbjct: 1   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 60

Query: 61  YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 120
            SILQTLKHSLSHALSHFLPLAGNL+WPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS
Sbjct: 61  DSILQTLKHSLSHALSHFLPLAGNLLWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 120

Query: 121 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 180
           NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS
Sbjct: 121 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 180

Query: 181 WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 240
           WASICSTLNNTNNKNPPTLPS+LTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP
Sbjct: 181 WASICSTLNNTNNKNPPTLPSQLTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 240

Query: 241 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 300
           KEVISDDVVYATFELT IDIEKLRRRVVATSSSTPRRLS+FMLAFSLVSTCIVKAQR EP
Sbjct: 241 KEVISDDVVYATFELTCIDIEKLRRRVVATSSSTPRRLSSFMLAFSLVSTCIVKAQRIEP 300

Query: 301 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE 360
           ESKIGLIFLVDWRARMDVLG LNYFGNGVNGYGLFVEARELEGENGM MISNKISD LAE
Sbjct: 301 ESKIGLIFLVDWRARMDVLGGLNYFGNGVNGYGLFVEARELEGENGMTMISNKISDELAE 360

Query: 361 IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 420
           IEKN+KENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS
Sbjct: 361 IEKNVKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 420

Query: 421 PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 465
           PNGVFSMAESRNGDGGVELGI+LPSEA+DKFCSLFSET+KGYVD
Sbjct: 421 PNGVFSMAESRNGDGGVELGIALPSEAMDKFCSLFSETVKGYVD 464

BLAST of CmoCh20G009170 vs. NCBI nr
Match: XP_022986520.1 (phenolic glucoside malonyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 799.7 bits (2064), Expect = 1.4e-227
Identity = 402/464 (86.64%), Postives = 423/464 (91.16%), Query Frame = 0

Query: 1   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHF 60
           MELPKKPWLEILEQCKIGPSPSPPTPFSLPLT FDLSFF +PPTQ ILFYSL PH+LLH 
Sbjct: 6   MELPKKPWLEILEQCKIGPSPSPPTPFSLPLTLFDLSFFSAPPTQHILFYSLSPHQLLHL 65

Query: 61  YSILQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSS 120
            SIL  LKHSLSHALSHFLPLAG+LVWPPQSP+PFI YNPGDS+SLTIAKTHADFH LSS
Sbjct: 66  DSILLNLKHSLSHALSHFLPLAGSLVWPPQSPDPFILYNPGDSVSLTIAKTHADFHLLSS 125

Query: 121 NHARKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKS 180
           NHARKA ESHFLVP LPTSDTIAP MSLQITLFPK GF IGIITNHVVSDAKTSTMFLKS
Sbjct: 126 NHARKATESHFLVPQLPTSDTIAPAMSLQITLFPKSGFCIGIITNHVVSDAKTSTMFLKS 185

Query: 181 WASICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTP 240
           WASICSTLNNTNNKNPPTLPSELTPCFDRT ATDPNGLHT+YVKSF IFV  PKLLGL P
Sbjct: 186 WASICSTLNNTNNKNPPTLPSELTPCFDRTSATDPNGLHTIYVKSFEIFV--PKLLGLAP 245

Query: 241 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 300
           KEVISDDVVYATFELT IDIEK+RRRVVATSSSTPRRL+T MLAFSL STCIVKAQR  P
Sbjct: 246 KEVISDDVVYATFELTCIDIEKVRRRVVATSSSTPRRLTTLMLAFSLASTCIVKAQRIAP 305

Query: 301 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAE 360
           E KIGLIFLVDWRARMD+LG LNYFGN V+ YG+F EARELE ENGMAMISNKIS+ + E
Sbjct: 306 ECKIGLIFLVDWRARMDMLGGLNYFGNCVSAYGVFAEARELEEENGMAMISNKISEEIEE 365

Query: 361 IEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASIS 420
           IEKN KENK VEML+ ISERW+K MPIDK ++VAGSPRLGVYDIDFGWGRSKKVE  SIS
Sbjct: 366 IEKNGKENKIVEMLEAISERWRKEMPIDKLIIVAGSPRLGVYDIDFGWGRSKKVEQVSIS 425

Query: 421 PNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 465
           PNGVFSMAESRNGDGGVELGI+LP +A+DKFCSLFSET+KGYVD
Sbjct: 426 PNGVFSMAESRNGDGGVELGIALPPQAMDKFCSLFSETVKGYVD 467

BLAST of CmoCh20G009170 vs. NCBI nr
Match: XP_022986173.1 (phenolic glucoside malonyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 390/456 (85.53%), Postives = 412/456 (90.35%), Query Frame = 0

Query: 9   LEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYSILQTLK 68
           +EILE CKI PSPSPPTPFSLPLT FDLSFF +PPTQ ILFYSL PH+LLH  SIL  LK
Sbjct: 3   MEILEVCKISPSPSPPTPFSLPLTLFDLSFFSAPPTQHILFYSLSPHQLLHLDSILLNLK 62

Query: 69  HSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARKAKE 128
           HSLSHALSHFLPLAG+LVWPPQSP+PFI YNPGDS+SLTIAKTHADFH LSSNHARKA E
Sbjct: 63  HSLSHALSHFLPLAGSLVWPPQSPDPFILYNPGDSVSLTIAKTHADFHLLSSNHARKATE 122

Query: 129 SHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICSTL 188
           SHFLVP LPTSDTIAP MSLQITLFPK GF IGIITNHVVSDAKTSTMFLKSWASICSTL
Sbjct: 123 SHFLVPQLPTSDTIAPAMSLQITLFPKSGFCIGIITNHVVSDAKTSTMFLKSWASICSTL 182

Query: 189 NNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTPKEVISDDV 248
           NNTNNKNPPTLPSELTPCFDRT ATDPNGLHT+YVKSF IFV  PKLLGL PKEVISDDV
Sbjct: 183 NNTNNKNPPTLPSELTPCFDRTSATDPNGLHTIYVKSFEIFV--PKLLGLAPKEVISDDV 242

Query: 249 VYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEPESKIGLIF 308
           VYATFELT IDIEK+RRRVVATSSSTPRRL+T MLAFSL STCIVKAQR  PE KIGLIF
Sbjct: 243 VYATFELTCIDIEKVRRRVVATSSSTPRRLTTLMLAFSLASTCIVKAQRIAPECKIGLIF 302

Query: 309 LVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAEIEKNMKEN 368
           LVDWRARMD+LG LNYFGN V+ YG+F EARELE ENGMAMISNKIS+ + EIEKN KEN
Sbjct: 303 LVDWRARMDMLGGLNYFGNCVSAYGVFAEARELEEENGMAMISNKISEEIEEIEKNGKEN 362

Query: 369 KGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASISPNGVFSMA 428
           K VEML+ ISERW+K MPIDK ++VAGSPRLGVYDIDFGWGRSKKVE  SISPNGVFSMA
Sbjct: 363 KIVEMLEAISERWRKEMPIDKLIIVAGSPRLGVYDIDFGWGRSKKVEQVSISPNGVFSMA 422

Query: 429 ESRNGDGGVELGISLPSEAVDKFCSLFSETMKGYVD 465
           ESRNGDGGVELGI+LP +A+DKFCSLFSET+K YVD
Sbjct: 423 ESRNGDGGVELGIALPPQAMDKFCSLFSETVKSYVD 456

BLAST of CmoCh20G009170 vs. TAIR 10
Match: AT5G39090.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 293.9 bits (751), Expect = 2.3e-79
Identity = 183/459 (39.87%), Postives = 264/459 (57.52%), Query Frame = 0

Query: 7   PWLEILEQCKIGPSPS-PPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYS-IL 66
           P L  +   ++ PS S      +LPLT+FDL +      +R++FY L       F S I+
Sbjct: 3   PSLNFIHVSRVTPSNSNSSASLTLPLTFFDLLWLKHKAVERVIFYKLTDVNRSLFDSVIV 62

Query: 67  QTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHAR 126
             LK SLS +LSH+LPLAG+++W P  P+P I Y   D++S T+A++++DF  L+     
Sbjct: 63  PNLKSSLSSSLSHYLPLAGHIIWEPHDPKPKIVYTQNDAVSFTVAESNSDFSLLTGKEPF 122

Query: 127 KAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASI 186
            + E H LVP+L  SD  A V+S Q+TLFP  GF IG+ T+H VSD KT+T FLKSWA +
Sbjct: 123 SSTELHPLVPELQNSDDSAAVVSFQVTLFPNQGFCIGVTTHHAVSDGKTTTTFLKSWAHL 182

Query: 187 CSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTPKEVI 246
           C   ++       +LP +L P +DRT    P  + T  +K +   +  PK L L P+  I
Sbjct: 183 CKHQDS-------SLPDDLIPFYDRTVIKGPPEIDTKVLKIWH-SIHKPKSLKLLPRPEI 242

Query: 247 SDDVVYATFELTRIDIEKLRRRVVATSSS-TPRRLSTFMLAFSLVSTCIVKAQRTEPESK 306
             DVV  TFELTR +IEKLR ++   SSS +  RLSTF++ FS V TC++ +   +P   
Sbjct: 243 ESDVVRYTFELTRENIEKLRDKLKRESSSFSSVRLSTFVITFSYVFTCLIGSGGDDPNRP 302

Query: 307 IGLIFLVDWRARMDVLGW-LNYFGNGV-NGYGLFVEARELEGENGMAMISNKISDALAEI 366
           +G  F VD R  +D     L YFGN V +   + ++A    GE G  + +  ISD++ E+
Sbjct: 303 VGYRFAVDCRRLIDDPPIPLTYFGNCVYSAVKIPLDAGMFLGEQGFVVAARLISDSVEEL 362

Query: 367 EKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASISP 426
           + N+        + E+ E ++KA    +FV VAGS R G+Y +DFGWG+  K  L SI  
Sbjct: 363 DSNV-----AWKIPELLETYEKAPVDSQFVSVAGSTRFGIYGLDFGWGKPFKSLLVSIDQ 422

Query: 427 NGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMK 461
            G  S+AESR+G GGVE+G SL  + ++    L  + +K
Sbjct: 423 RGKISIAESRDGSGGVEIGFSLKKQEMNVLIDLLHKGIK 448

BLAST of CmoCh20G009170 vs. TAIR 10
Match: AT5G39080.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 285.4 bits (729), Expect = 8.1e-77
Identity = 183/466 (39.27%), Postives = 255/466 (54.72%), Query Frame = 0

Query: 9   LEILEQCKIGPSPSPPT-PFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYS-ILQT 68
           L I+E  ++ PS S      SLPLTYFDL ++     +R++FY +       F S I+  
Sbjct: 5   LNIIEVARVTPSNSDSAESLSLPLTYFDLIYYKLRAVERVIFYRITNVTRPFFDSVIVPN 64

Query: 69  LKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARKA 128
           LK SLS  LSH+LPLAG L+W P   +P I Y+  D +S ++A+T+ADF  LS N    +
Sbjct: 65  LKTSLSSCLSHYLPLAGKLIWEPLDHKPTIVYSQNDDVSFSVAETNADFSSLSGNEPFPS 124

Query: 129 KESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICS 188
            E + LVP L +SD  A ++S Q+TLFP  GF IG+  +H V D KT+TMFLKSWA IC 
Sbjct: 125 TELYPLVPALQSSDDSASIVSFQVTLFPNQGFCIGVSAHHAVLDGKTTTMFLKSWAHIC- 184

Query: 189 TLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFV---------PNPKLLG 248
                      +LP +L P +DRT    P       +  +  F           NPK L 
Sbjct: 185 ------KHQDFSLPQDLIPTYDRTVIKSPTDSENKVLNEWRSFTKILAGGKEPANPKSLK 244

Query: 249 LTPKEVISDDVVYATFELTRIDIEKLRRRV--------VATSSSTPRRLSTFMLAFSLVS 308
           L P   I  DVV  T +LTR DI+ LR R+         +TSSS   RLSTF++ +S V 
Sbjct: 245 LNPSFEIGPDVVRYTLQLTREDIQTLRERLKREVSSSSSSTSSSKELRLSTFVIVYSYVL 304

Query: 309 TCIVKAQRTEPESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYG-LFVEARELEGENGMA 368
            CI++A+  EP   +G  F VD R+ M+     NYFGN + G   + + A+   GE G+ 
Sbjct: 305 VCIIRARGGEPHRPVGYAFSVDCRSLMNP-PTPNYFGNCIAGCSRMMLTAKMFMGEEGLL 364

Query: 369 MISNKISDALAEIEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGW 428
             +  +SD++ E +    E+   ++ D ++  +    P  + +LV+GS R GVY++DFGW
Sbjct: 365 AAATMVSDSIEEWD----ESFAWKIPDFVA--YATLPPETQLILVSGSNRFGVYELDFGW 424

Query: 429 GRSKKVELASISPNGVFSMAESRNGDGGVELGISLPSEAVDKFCSL 455
           GR  KV + SISP    SMAESR+ +G VE+G SL    +D    L
Sbjct: 425 GRPDKVMVVSISPGNGISMAESRDQNGSVEIGFSLKKHEMDTLIDL 456

BLAST of CmoCh20G009170 vs. TAIR 10
Match: AT3G29590.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 283.9 bits (725), Expect = 2.4e-76
Identity = 176/452 (38.94%), Postives = 251/452 (55.53%), Query Frame = 0

Query: 9   LEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYSILQTLK 68
           + ILE  ++ P  S     +LPLTYFDL +    P  R+LFY +P    L   S++  LK
Sbjct: 8   VNILEVVQVSPPSS--NSLTLPLTYFDLGWLKLHPVDRVLFYHVPE---LTRSSLISKLK 67

Query: 69  HSLSHALSHFLPLAGNLVWPPQSPEPFICYNPG--DSLSLTIAKTHADFHHLSSNHARKA 128
            SLS  L H+LPLAG LVW     +P I Y+P   D++ LT+A+++ D  HLS +  R A
Sbjct: 68  SSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEPRPA 127

Query: 129 KESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICS 188
            E H LVP+LP SD  A V+++Q+T FP  GFS+G+  +H V D KT+ MFLK+WA  C 
Sbjct: 128 TEFHSLVPELPVSDESARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAWAHNC- 187

Query: 189 TLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSFAIFVPNPKLLGLTPKEVISD 248
                  +    LP +L P  DR    DP GL T  +  +     N   L L P ++I  
Sbjct: 188 ------KQEQEALPHDLVPSLDRIIVQDPTGLETKLLNRWISASNNKPSLKLFPSKIIGS 247

Query: 249 DVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEPESKIGL 308
           D++  T+ LTR DI+KLR RV   S +   RLSTF++ ++ V TC+VK +  +P   + +
Sbjct: 248 DILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVCV 307

Query: 309 IFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARE--LEGENGMAMISNKISDALAEIEKN 368
            F  D+R+R++      +FGN + G G F    E  LE   G   I+  +      +   
Sbjct: 308 GFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFIT-AVETLTGWVNGL 367

Query: 369 MKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASISPNGV 428
             EN    ML    E +K+  P  + + VAGS RLG+Y  DFGWG+  KVE+ +I  +  
Sbjct: 368 CPENIEKNMLLPF-EAFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKDAS 427

Query: 429 FSMAESRNGDGGVELGISLPSEAVDKFCSLFS 457
            S++ES +G GGVE+G+ L  + V++F SLFS
Sbjct: 428 VSLSESGDGSGGVEVGVCLKKDDVERFGSLFS 445

BLAST of CmoCh20G009170 vs. TAIR 10
Match: AT5G39050.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 276.6 bits (706), Expect = 3.8e-74
Identity = 178/471 (37.79%), Postives = 256/471 (54.35%), Query Frame = 0

Query: 9   LEILEQCKIGPSPSPPT-PFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYS-ILQT 68
           L++++  ++ PS S  +   +LPLT+FDL ++     +R++FY L       F S I+  
Sbjct: 10  LKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIVPN 69

Query: 69  LKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARKA 128
           LK SLS +LSH+LPLAG LVW P  P+P I Y P D++S T+A+++ADF  L+       
Sbjct: 70  LKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPFPT 129

Query: 129 KESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICS 188
            E + LVP+L  SD  A  +S Q+TLFP  GF I +  +H V D KT+T FLKSWA  C 
Sbjct: 130 TELYPLVPELHVSDDSASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWARTCK 189

Query: 189 TLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTLYVKSF----AIFV-----PNPKLLG 248
             ++        LP +L P +DRT   DP  L T  + ++     +F       NPK L 
Sbjct: 190 NQDS-------FLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKSLK 249

Query: 249 LTPKEVISDDVVYATFELTRIDIEKLRRRVVATS-----SSTPR--RLSTFMLAFSLVST 308
           L     I  DV   T  LTR DI+KLR R+   S     SS+P+  RLSTF++ +S   T
Sbjct: 250 LLWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALT 309

Query: 309 CIVKAQRTEPESKIGLIFLVDWRARMDVLGWLNYFGNGVNG-YGLFVEARELEGENGMAM 368
           C++KA+  +P   +G  F VD R+ M      +YFGN V+  + + + A     E G   
Sbjct: 310 CLIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLA 369

Query: 369 ISNKISDALAEIEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWG 428
            +  +SD++  +++N+        + EI E +    P  + + VAGS R GVY +DFGWG
Sbjct: 370 AARMVSDSVEALDENV-----ALKIPEILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWG 429

Query: 429 RSKKVELASISPNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMK 461
           R +KV + SI      S AESR+G GGVELG SL    +D    L  + ++
Sbjct: 430 RPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLE 468

BLAST of CmoCh20G009170 vs. TAIR 10
Match: AT3G29670.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 267.3 bits (682), Expect = 2.3e-71
Identity = 173/457 (37.86%), Postives = 241/457 (52.74%), Query Frame = 0

Query: 9   LEILEQCKIGPSP----SPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHELLHFYS-I 68
           L ++E  ++ P+     +      LPLT+FDL + L  P +R+ FY L      HF+S I
Sbjct: 3   LHVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHFHSII 62

Query: 69  LQTLKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHA 128
           L  LK SLS  L ++LPL G++ W P  P+P I  +    + +TIA++ ADF HLS    
Sbjct: 63  LPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAESDADFSHLSGYGQ 122

Query: 129 RKAKESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWAS 188
           R   E H LVP LP SD  A   S+QITLFP  GFSIG+  +H V D KTS+ F+K+WA 
Sbjct: 123 RPLSELHALVPKLPVSDDSATAFSIQITLFPNQGFSIGVAAHHAVLDGKTSSTFIKAWAQ 182

Query: 189 ICSTLNNTNNKNPPTLPSELTPCFDRTPATDPNGLHTL---YVKSFAIFVPNPKLLGLTP 248
           IC        +   ++P  LTP +DR+    P  L       V+S      N + L   P
Sbjct: 183 IC-------KQELQSMPENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLTSLP 242

Query: 249 KEVISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEP 308
              + DDVV AT  L+R DIE+LR +V   S S    LSTF++A++   TC VKA+    
Sbjct: 243 SSKLGDDVVLATLVLSRADIERLREQVKNVSPSL--HLSTFVIAYAYAWTCFVKARGGNK 302

Query: 309 ESKIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLF-VEARELEGENGMAMISNKISDALA 368
           +  + L+F+ D+R R+D      YFGN +   G +  +A E   E G    +  ISD   
Sbjct: 303 DRSVSLLFVGDFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAAEIISD--- 362

Query: 369 EIEKNMKENKGVEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASI 428
            + K +   K   + D   E +       +F  +AGS RLGVY+ DFGWGR  KV++ SI
Sbjct: 363 -LVKGLSSRKIETIADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVDIVSI 422

Query: 429 SPNGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFS 457
                 +MAE R+  GGVE+G+ L    +D   S F+
Sbjct: 423 DQGEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFN 446

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LJB43.3e-7538.94Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase OS=Arabidopsis ... [more]
Q940Z55.3e-7337.79Phenolic glucoside malonyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PMAT1... [more]
Q9LRQ83.2e-7037.86Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2... [more]
Q9LRQ73.0e-6836.99BAHD acyltransferase At3g29680 OS=Arabidopsis thaliana OX=3702 GN=At3g29680 PE=2... [more]
Q9FNP91.3e-6334.72Agmatine coumaroyltransferase OS=Arabidopsis thaliana OX=3702 GN=ACT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FVL41.3e-268100.00malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbi... [more]
A0A6J1JEA36.6e-22886.64phenolic glucoside malonyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1JFR05.1e-22085.53phenolic glucoside malonyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FXM37.7e-18473.03malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbi... [more]
A0A6J1J6U62.0e-17971.43malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbi... [more]
Match NameE-valueIdentityDescription
XP_022944412.12.7e-268100.00malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like [Cucurbita... [more]
KAG6571175.13.2e-26197.63Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, partial [Cucur... [more]
KAG7010980.13.6e-26097.20Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, partial [Cucur... [more]
XP_022986520.11.4e-22786.64phenolic glucoside malonyltransferase 1-like [Cucurbita maxima][more]
XP_022986173.11.1e-21985.53phenolic glucoside malonyltransferase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G39090.12.3e-7939.87HXXXD-type acyl-transferase family protein [more]
AT5G39080.18.1e-7739.27HXXXD-type acyl-transferase family protein [more]
AT3G29590.12.4e-7638.94HXXXD-type acyl-transferase family protein [more]
AT5G39050.13.8e-7437.79HXXXD-type acyl-transferase family protein [more]
AT3G29670.12.3e-7137.86HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 245..461
e-value: 3.4E-39
score: 136.2
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 5..229
e-value: 6.8E-62
score: 210.9
IPR003480TransferasePFAMPF02458Transferasecoord: 12..455
e-value: 7.5E-45
score: 153.4
NoneNo IPR availablePANTHERPTHR31625FAMILY NOT NAMEDcoord: 9..460
NoneNo IPR availablePANTHERPTHR31625:SF655-AROMATIC ACYLTRANSFERASE, PUTATIVE-RELATEDcoord: 9..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G009170.1CmoCh20G009170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups