CmoCh20G009130 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G009130
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionInositol-tetrakisphosphate 1-kinase
LocationCmo_Chr20: 4688875 .. 4689906 (+)
RNA-Seq ExpressionCmoCh20G009130
SyntenyCmoCh20G009130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTCTTCTTCTTCTTCGTCGAATCGCCTTCGAGTAGGCTACGCTTTTCCTCCTAACAAAGAGCGCAATGTGATTCGCCCTTCTCTCGTCGATTACGCCAAGCTCCATGGTGTCGACCTGGTCCGTATCGACCTCCAAACGCCGCTCCTCCACCAAGGTCCATTTCATTGCATTATTCACAAACTCTATGACGATGCTTGGGCGGAGCATCTGCAGGACTTCGCCTTGAAGAATCCGGACGTCGTTGTCGTGGATCGTCCGGACCTAATCGCTCGACTCTACGACAGAGTCTCCATGCTTGATGTGGTAAGCCAGGTGAAGGCTTCGGACAGCGGCGTGAGGATCGAGGTGCCGAAACAGATCGTTCTGAAACAGGAGGATGGAGAAATCGATTCGATTAGGGATTTGGATGTGAAATTTCCAGTGATTGCGAAACCGTTGGCGTCGGATGGAAGTGCGAAATCGCATGAAATGTCTCTGGTTTTCAATCGACGCGGATTAAAGGATTTGGATAGGCCAGTGCTTCTACAGGAGTTTGTGAACCACGGCGGAGTAATGTTCAAAGTATACGTCGCTGGAGAGGAATCCGTGTGCGTGAAGCGGAAATCGTTGCCGGATGTGGCGGAGACAGAGGAGGAACTGGAGAAGAAACAAGACGGCGCGATGAAATTTTCGCAAATATCTAGGGCAGAGGAGAAGAGCGAAAAATGTAACGGCGCTGAGAAGAAGGAGGCGGAGGAGATCGAAATGCCGCCGGAGAAGATAGTTAGGGAGGTATCGAATGGGCTGAGGGAAGCGATGGGGATTAGGCTGTTCAATTTCGACATGATTAGGGACGGGAATGGTACATACTATGTGATAGACATAAATTACTTACCAGGATTTGCAGTACTGCCGGAGTACGAGCAATTTCTGACGAAGTTCTTCAAGGAAGTTGCAGAGAAGAAGAAGAAGAAGAAGGTGGAAGAGGATGATTCCGCCATTGAAGACGGTAAAGCAAAACCGCGGTGCTGCTGTTTCCAATGA

mRNA sequence

ATGGCTTCTTCTTCTTCTTCTTCGTCGAATCGCCTTCGAGTAGGCTACGCTTTTCCTCCTAACAAAGAGCGCAATGTGATTCGCCCTTCTCTCGTCGATTACGCCAAGCTCCATGGTGTCGACCTGGTCCGTATCGACCTCCAAACGCCGCTCCTCCACCAAGGTCCATTTCATTGCATTATTCACAAACTCTATGACGATGCTTGGGCGGAGCATCTGCAGGACTTCGCCTTGAAGAATCCGGACGTCGTTGTCGTGGATCGTCCGGACCTAATCGCTCGACTCTACGACAGAGTCTCCATGCTTGATGTGGTAAGCCAGGTGAAGGCTTCGGACAGCGGCGTGAGGATCGAGGTGCCGAAACAGATCGTTCTGAAACAGGAGGATGGAGAAATCGATTCGATTAGGGATTTGGATGTGAAATTTCCAGTGATTGCGAAACCGTTGGCGTCGGATGGAAGTGCGAAATCGCATGAAATGTCTCTGGTTTTCAATCGACGCGGATTAAAGGATTTGGATAGGCCAGTGCTTCTACAGGAGTTTGTGAACCACGGCGGAGTAATGTTCAAAGTATACGTCGCTGGAGAGGAATCCGTGTGCGTGAAGCGGAAATCGTTGCCGGATGTGGCGGAGACAGAGGAGGAACTGGAGAAGAAACAAGACGGCGCGATGAAATTTTCGCAAATATCTAGGGCAGAGGAGAAGAGCGAAAAATGTAACGGCGCTGAGAAGAAGGAGGCGGAGGAGATCGAAATGCCGCCGGAGAAGATAGTTAGGGAGGTATCGAATGGGCTGAGGGAAGCGATGGGGATTAGGCTGTTCAATTTCGACATGATTAGGGACGGGAATGGTACATACTATGTGATAGACATAAATTACTTACCAGGATTTGCAGTACTGCCGGAGTACGAGCAATTTCTGACGAAGTTCTTCAAGGAAGTTGCAGAGAAGAAGAAGAAGAAGAAGGTGGAAGAGGATGATTCCGCCATTGAAGACGGTAAAGCAAAACCGCGGTGCTGCTGTTTCCAATGA

Coding sequence (CDS)

ATGGCTTCTTCTTCTTCTTCTTCGTCGAATCGCCTTCGAGTAGGCTACGCTTTTCCTCCTAACAAAGAGCGCAATGTGATTCGCCCTTCTCTCGTCGATTACGCCAAGCTCCATGGTGTCGACCTGGTCCGTATCGACCTCCAAACGCCGCTCCTCCACCAAGGTCCATTTCATTGCATTATTCACAAACTCTATGACGATGCTTGGGCGGAGCATCTGCAGGACTTCGCCTTGAAGAATCCGGACGTCGTTGTCGTGGATCGTCCGGACCTAATCGCTCGACTCTACGACAGAGTCTCCATGCTTGATGTGGTAAGCCAGGTGAAGGCTTCGGACAGCGGCGTGAGGATCGAGGTGCCGAAACAGATCGTTCTGAAACAGGAGGATGGAGAAATCGATTCGATTAGGGATTTGGATGTGAAATTTCCAGTGATTGCGAAACCGTTGGCGTCGGATGGAAGTGCGAAATCGCATGAAATGTCTCTGGTTTTCAATCGACGCGGATTAAAGGATTTGGATAGGCCAGTGCTTCTACAGGAGTTTGTGAACCACGGCGGAGTAATGTTCAAAGTATACGTCGCTGGAGAGGAATCCGTGTGCGTGAAGCGGAAATCGTTGCCGGATGTGGCGGAGACAGAGGAGGAACTGGAGAAGAAACAAGACGGCGCGATGAAATTTTCGCAAATATCTAGGGCAGAGGAGAAGAGCGAAAAATGTAACGGCGCTGAGAAGAAGGAGGCGGAGGAGATCGAAATGCCGCCGGAGAAGATAGTTAGGGAGGTATCGAATGGGCTGAGGGAAGCGATGGGGATTAGGCTGTTCAATTTCGACATGATTAGGGACGGGAATGGTACATACTATGTGATAGACATAAATTACTTACCAGGATTTGCAGTACTGCCGGAGTACGAGCAATTTCTGACGAAGTTCTTCAAGGAAGTTGCAGAGAAGAAGAAGAAGAAGAAGGTGGAAGAGGATGATTCCGCCATTGAAGACGGTAAAGCAAAACCGCGGTGCTGCTGTTTCCAATGA

Protein sequence

MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ
Homology
BLAST of CmoCh20G009130 vs. ExPASy Swiss-Prot
Match: Q9SBA5 (Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 7.4e-72
Identity = 148/319 (46.39%), Postives = 211/319 (66.14%), Query Frame = 0

Query: 5   SSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIHKL 64
           S S   R  VGYA    K+ + I+PSL+++++  G+DLV++D    LL QG   CIIHKL
Sbjct: 2   SDSIQERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKL 61

Query: 65  YDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVK--ASDSGVRIEVPKQ 124
           YD  W E+L +F  K P V V+D P+ I RL++RVSML+V++Q++   SDS  R  VP+Q
Sbjct: 62  YDVYWKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDS-ERFGVPEQ 121

Query: 125 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFV 184
           +V+             ++KFPVIAKPL +DGSAKSH+M L++++ G+K L  P++LQEFV
Sbjct: 122 VVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFV 181

Query: 185 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEK-CNG 244
           NHGGV+FKVYV G+   CVKR+SLPD++E +    K   G++ FSQIS    + +K    
Sbjct: 182 NHGGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSK---GSLPFSQISNLTAQEDKNIEY 241

Query: 245 AEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGN--GTYYVIDINYLPGFAV 304
            E +  E++EMPP   + +++  +RE+MG+ LFNFD+IRD      Y +IDINY PG+A 
Sbjct: 242 GEDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAK 301

Query: 305 LPEYEQFLTKFFKEVAEKK 319
           +P YE  LT+FF ++  KK
Sbjct: 302 MPSYEPVLTEFFWDMVTKK 316

BLAST of CmoCh20G009130 vs. ExPASy Swiss-Prot
Match: Q84Y01 (Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.2e-69
Identity = 152/332 (45.78%), Postives = 207/332 (62.35%), Query Frame = 0

Query: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIH 62
           SS  +   R  +GYA  P K+++ I+PSLV  A   G+DLV +D   PL  QGPFH +IH
Sbjct: 10  SSGVTHPPRYVIGYALAPKKQQSFIQPSLVAQAASRGMDLVPVDASQPLAEQGPFHLLIH 69

Query: 63  KLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQV-KASDSGVRIEVPK 122
           KLY D W   L  FA ++P V +VD P  I RL++R+SML VVS++  A+D      +P 
Sbjct: 70  KLYGDDWRAQLVAFAARHPAVPIVDPPHAIDRLHNRISMLQVVSELDHAADQDSTFGIPS 129

Query: 123 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEF 182
           Q+V+       D      ++FP+IAKPL +DG+AKSH+MSLV++R GL  L  P++LQEF
Sbjct: 130 QVVVYDAAALADFGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLGKLRPPLVLQEF 189

Query: 183 VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISR--AEEKSEKC 242
           VNHGGV+FKVYV G    CVKR+SLPDV+    E +    G++ FSQ+S    E  +E+ 
Sbjct: 190 VNHGGVIFKVYVVGGHVTCVKRRSLPDVS---PEDDASAQGSVSFSQVSNLPTERTAEEY 249

Query: 243 NGAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRD--GNGTYYVIDINYLPGF 302
            G  +K  E+  +PP   + +++ GLR A+G++LFNFDMIRD      Y VIDINY PG+
Sbjct: 250 YG--EKSLEDAVVPPAAFINQIAGGLRRALGLQLFNFDMIRDVRAGDRYLVIDINYFPGY 309

Query: 303 AVLPEYEQFLTKFFKEVAEKKKKKKVEEDDSA 330
           A +P YE  LT FF E+  K      +E+  A
Sbjct: 310 AKMPGYETVLTDFFWEMVHKDGVGNQQEEKGA 336

BLAST of CmoCh20G009130 vs. ExPASy Swiss-Prot
Match: A0JJZ6 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK5 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 3.2e-67
Identity = 141/312 (45.19%), Postives = 194/312 (62.18%), Query Frame = 0

Query: 11  RLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 70
           R  +GYA  P K+++ I+PSLV  A   G+DLV +D   PL  QGPFH +IHKLY + W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 71  EHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKA---SDSGVRIEVPKQIVLKQ 130
             L  F+  +P V VVD P  I RL++R+SML VVS++     +       +P Q+V+  
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 131 EDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFVNHGGV 190
                DS     ++FP+IAKPL +DG+AKSH+MSLV++R GL+ L  P++LQEFVNHGGV
Sbjct: 135 AAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGV 194

Query: 191 MFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGAEKKEA 250
           +FKVYV G    CVKR+SLPDV+ ++   +   +G++ FSQ+S    +       +    
Sbjct: 195 IFKVYVVGAHVTCVKRRSLPDVS-SDVLQDASAEGSLSFSQVSNLPNERTAQEYYDDMRL 254

Query: 251 EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNG--TYYVIDINYLPGFAVLPEYEQ 310
           E+  MPP   + +++  LR A+G+ LFNFDMIRD      Y VIDINY PG+A +P YE 
Sbjct: 255 EDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPGYET 314

Query: 311 FLTKFFKEVAEK 318
            LT FF E+  K
Sbjct: 315 VLTDFFWEMVHK 325

BLAST of CmoCh20G009130 vs. ExPASy Swiss-Prot
Match: Q7XBW0 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=ITPK5 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 3.2e-67
Identity = 141/312 (45.19%), Postives = 194/312 (62.18%), Query Frame = 0

Query: 11  RLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 70
           R  +GYA  P K+++ I+PSLV  A   G+DLV +D   PL  QGPFH +IHKLY + W 
Sbjct: 15  RYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWR 74

Query: 71  EHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKA---SDSGVRIEVPKQIVLKQ 130
             L  F+  +P V VVD P  I RL++R+SML VVS++     +       +P Q+V+  
Sbjct: 75  GQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYD 134

Query: 131 EDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFVNHGGV 190
                DS     ++FP+IAKPL +DG+AKSH+MSLV++R GL+ L  P++LQEFVNHGGV
Sbjct: 135 AAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGV 194

Query: 191 MFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGAEKKEA 250
           +FKVYV G    CVKR+SLPDV+ ++   +   +G++ FSQ+S    +       +    
Sbjct: 195 IFKVYVVGAHVTCVKRRSLPDVS-SDVLQDASAEGSLSFSQVSNLPNERTAQEYYDDMRL 254

Query: 251 EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNG--TYYVIDINYLPGFAVLPEYEQ 310
           E+  MPP   + +++  LR A+G+ LFNFDMIRD      Y VIDINY PG+A +P YE 
Sbjct: 255 EDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPGYET 314

Query: 311 FLTKFFKEVAEK 318
            LT FF E+  K
Sbjct: 315 VLTDFFWEMVHK 325

BLAST of CmoCh20G009130 vs. ExPASy Swiss-Prot
Match: O81893 (Inositol-tetrakisphosphate 1-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ITPK2 PE=1 SV=3)

HSP 1 Score: 245.7 bits (626), Expect = 7.4e-64
Identity = 132/315 (41.90%), Postives = 197/315 (62.54%), Query Frame = 0

Query: 11  RLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 70
           +L VGYA    K+++ ++P L   A+  G+  V IDL  PL  QGPF  ++HKL    W 
Sbjct: 80  KLVVGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWE 139

Query: 71  EHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQ-IVLKQED 130
           E ++D+  K+P+V V+D P  I R+Y+R SML  ++ +K SD    + VPKQ +VLK   
Sbjct: 140 EVIEDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVLKDSA 199

Query: 131 GEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFVNHGGVMF 190
              D++ +  +KFP++AKPL  DG+AKSH++ L ++RR L +LD P++LQEFVNHGGVMF
Sbjct: 200 ASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMF 259

Query: 191 KVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGAEKKEAEE 250
           KV+V G+    ++R SLP+V+  E   + K DG  +F ++S A   ++  +     +   
Sbjct: 260 KVFVVGDVIKVMRRFSLPNVSNCE---KAKVDGVFQFPRVSSAAASADNAD----LDPRV 319

Query: 251 IEMPPEKIVREVSNGLREAMGIRLFNFDMIRD--GNGTYYVIDINYLPGFAVLPEYEQFL 310
            E+PP+  +  +   LR  +G+RLFN DMIR+      +YVIDINY PG+  LP+YEQ  
Sbjct: 320 AELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDYEQVF 379

Query: 311 TKFFKEVAEKKKKKK 323
             FF+ +A+ K KK+
Sbjct: 380 VDFFQNLAQVKYKKR 387

BLAST of CmoCh20G009130 vs. ExPASy TrEMBL
Match: A0A6J1FTQ5 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC111448363 PE=3 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.0e-189
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120
           IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240

Query: 241 GAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVL 300
           GAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVL
Sbjct: 241 GAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVL 300

Query: 301 PEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 344
           PEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 301 PEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 343

BLAST of CmoCh20G009130 vs. ExPASy TrEMBL
Match: A0A6J1JBH8 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111484264 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 5.0e-180
Identity = 331/347 (95.39%), Postives = 336/347 (96.83%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRID+QTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120
           IHKLYDDAWAEHLQDFA KNPDVVVVDRPDLIA+LYDRVSMLDVVSQVK SDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDL+ PVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDV ETEEELEKK DGAMKFSQISRAEEKSEKC+
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD 240

Query: 241 GAEKKEA----EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300
           GAEKK A    EEIEMPPEKIVREVSNGL+EAMGIRLFNFDMIRDGNGTYYVIDINYLPG
Sbjct: 241 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300

Query: 301 FAVLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 344
           FAVLPEYEQFLTKFFKEVAE KKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 301 FAVLPEYEQFLTKFFKEVAE-KKKKKVEEDDSAIEDGKAKPRCCCFQ 346

BLAST of CmoCh20G009130 vs. ExPASy TrEMBL
Match: A0A5A7TY89 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold66G00690 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.2e-159
Identity = 292/342 (85.38%), Postives = 316/342 (92.40%), Query Frame = 0

Query: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIH 62
           +S+S SSNRLRVGYAFPPNKERNVIRPSL+DYAK HGVDLVRIDLQTPLLHQGPFHCIIH
Sbjct: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKFHGVDLVRIDLQTPLLHQGPFHCIIH 61

Query: 63  KLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQ 122
           KLYDDAWAE+LQDFA KNPDVVVVDRPDLI++LY+RVSMLDVVSQVK SDSGVRIEVPKQ
Sbjct: 62  KLYDDAWAENLQDFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSGVRIEVPKQ 121

Query: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFV 182
           I++K+E+  IDSI +L++KFPVIAKP+ SDGSAKSHEMSLVFNRRGLK+L+ PVLLQEFV
Sbjct: 122 ILVKKEEEAIDSIMNLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKELNTPVLLQEFV 181

Query: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGA 242
           NHGGVMFK+YVAG++SVCVKRKSLPDV  TEEELEKK +GAMKFSQISRAEEKSEKCNG 
Sbjct: 182 NHGGVMFKIYVAGDQSVCVKRKSLPDVEGTEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241

Query: 243 EKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLPE 302
            KKE +EIEMPPEKIVREVS GL+EAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLPE
Sbjct: 242 AKKEEKEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLPE 301

Query: 303 YEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCC-CFQ 344
           YE FLTKFFKEV E    KKV+E  SAIED KAKPRCC CFQ
Sbjct: 302 YEPFLTKFFKEVTE----KKVDEVASAIEDDKAKPRCCSCFQ 339

BLAST of CmoCh20G009130 vs. ExPASy TrEMBL
Match: A0A1S3C9X3 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo OX=3656 GN=LOC103498455 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.2e-159
Identity = 292/342 (85.38%), Postives = 316/342 (92.40%), Query Frame = 0

Query: 3   SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIH 62
           +S+S SSNRLRVGYAFPPNKERNVIRPSL+DYAK HGVDLVRIDLQTPLLHQGPFHCIIH
Sbjct: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKFHGVDLVRIDLQTPLLHQGPFHCIIH 61

Query: 63  KLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQ 122
           KLYDDAWAE+LQDFA KNPDVVVVDRPDLI++LY+RVSMLDVVSQVK SDSGVRIEVPKQ
Sbjct: 62  KLYDDAWAENLQDFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSGVRIEVPKQ 121

Query: 123 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFV 182
           I++K+E+  IDSI +L++KFPVIAKP+ SDGSAKSHEMSLVFNRRGLK+L+ PVLLQEFV
Sbjct: 122 ILVKKEEEAIDSIMNLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKELNTPVLLQEFV 181

Query: 183 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGA 242
           NHGGVMFK+YVAG++SVCVKRKSLPDV  TEEELEKK +GAMKFSQISRAEEKSEKCNG 
Sbjct: 182 NHGGVMFKIYVAGDQSVCVKRKSLPDVEGTEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241

Query: 243 EKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLPE 302
            KKE +EIEMPPEKIVREVS GL+EAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLPE
Sbjct: 242 AKKEEKEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLPE 301

Query: 303 YEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCC-CFQ 344
           YE FLTKFFKEV E    KKV+E  SAIED KAKPRCC CFQ
Sbjct: 302 YEPFLTKFFKEVTE----KKVDEVASAIEDDKAKPRCCSCFQ 339

BLAST of CmoCh20G009130 vs. ExPASy TrEMBL
Match: A0A6J1G5K0 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC111451086 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.5e-157
Identity = 290/344 (84.30%), Postives = 312/344 (90.70%), Query Frame = 0

Query: 2   ASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCII 61
           +SSSSSSS+  RVGYAFPPNKERNVIRPSL+DYAKLHGVDLVRIDLQ+P+LHQGPFHCII
Sbjct: 6   SSSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQSPILHQGPFHCII 65

Query: 62  HKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPK 121
           HK+YDDAW E LQDFALKNPDVVVVDRPD I RLY+RVSMLDVVSQVK SDSGV+IEVPK
Sbjct: 66  HKMYDDAWVEKLQDFALKNPDVVVVDRPDRIERLYNRVSMLDVVSQVKVSDSGVKIEVPK 125

Query: 122 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEF 181
           Q+VL ++DG ID IRDL+VKFPVIAKPL S+GSAKSHEMSLV+NRRG KDL+ PVLLQEF
Sbjct: 126 QVVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEF 185

Query: 182 VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNG 241
           VNHGGVMFKVYVAGE+SVCVKRKSLPDV ETEEELEKK DGAMKFSQISRAEEKSEKCN 
Sbjct: 186 VNHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNC 245

Query: 242 AEKKEA---EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFA 301
             KKEA   EEIEMPPEKIV EVS GLREAMGIRLFNFDMIRD NGTYYVIDINYLPGFA
Sbjct: 246 GRKKEAAAEEEIEMPPEKIVTEVSRGLREAMGIRLFNFDMIRDRNGTYYVIDINYLPGFA 305

Query: 302 VLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCF 343
           VLP+YE FLTKFF EV +KK +   EE++SAIE+ KAKPRCCCF
Sbjct: 306 VLPDYEAFLTKFFNEVMQKKVE---EEENSAIEEEKAKPRCCCF 346

BLAST of CmoCh20G009130 vs. NCBI nr
Match: XP_022943687.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita moschata])

HSP 1 Score: 671.4 bits (1731), Expect = 4.2e-189
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120
           IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240

Query: 241 GAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVL 300
           GAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVL
Sbjct: 241 GAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVL 300

Query: 301 PEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 344
           PEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 301 PEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 343

BLAST of CmoCh20G009130 vs. NCBI nr
Match: KAG6571172.1 (Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 664.5 bits (1713), Expect = 5.1e-187
Identity = 339/342 (99.12%), Postives = 340/342 (99.42%), Query Frame = 0

Query: 2   ASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCII 61
           +SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCII
Sbjct: 3   SSSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCII 62

Query: 62  HKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPK 121
           HKLYDDAWAEHLQDFA KNPDVVVVDRPDLIARLYDRVSMLDVVSQVK SDSGVRIEVPK
Sbjct: 63  HKLYDDAWAEHLQDFASKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKGSDSGVRIEVPK 122

Query: 122 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEF 181
           QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEF
Sbjct: 123 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEF 182

Query: 182 VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNG 241
           VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNG
Sbjct: 183 VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNG 242

Query: 242 AEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLP 301
           AEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLP
Sbjct: 243 AEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVLP 302

Query: 302 EYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 344
           EYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 303 EYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 344

BLAST of CmoCh20G009130 vs. NCBI nr
Match: XP_023512946.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650.6 bits (1677), Expect = 7.6e-183
Identity = 335/346 (96.82%), Postives = 338/346 (97.69%), Query Frame = 0

Query: 2   ASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCII 61
           +SSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCII
Sbjct: 4   SSSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCII 63

Query: 62  HKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPK 121
           HKLYDDAWAEHLQDFA KNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPK
Sbjct: 64  HKLYDDAWAEHLQDFASKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPK 123

Query: 122 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEF 181
           QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDL+ PVLLQEF
Sbjct: 124 QIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQEF 183

Query: 182 VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNG 241
           VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKK DGAMKFSQISRAEEKSEKCNG
Sbjct: 184 VNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKPDGAMKFSQISRAEEKSEKCNG 243

Query: 242 AEKK----EAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGF 301
           AEKK    E EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGF
Sbjct: 244 AEKKAAAEEEEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGF 303

Query: 302 AVLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 344
           AVLPEYEQFLTKFFKEVAEK+KKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 304 AVLPEYEQFLTKFFKEVAEKEKKKKVEEDDSAIEDGKAKPRCCCFQ 349

BLAST of CmoCh20G009130 vs. NCBI nr
Match: XP_022986561.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima])

HSP 1 Score: 640.2 bits (1650), Expect = 1.0e-179
Identity = 331/347 (95.39%), Postives = 336/347 (96.83%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRID+QTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDIQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120
           IHKLYDDAWAEHLQDFA KNPDVVVVDRPDLIA+LYDRVSMLDVVSQVK SDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIAQLYDRVSMLDVVSQVKVSDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDL+ PVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLETPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDV ETEEELEKK DGAMKFSQISRAEEKSEKC+
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVTETEEELEKKPDGAMKFSQISRAEEKSEKCD 240

Query: 241 GAEKKEA----EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300
           GAEKK A    EEIEMPPEKIVREVSNGL+EAMGIRLFNFDMIRDGNGTYYVIDINYLPG
Sbjct: 241 GAEKKAAAEKEEEIEMPPEKIVREVSNGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPG 300

Query: 301 FAVLPEYEQFLTKFFKEVAEKKKKKKVEEDDSAIEDGKAKPRCCCFQ 344
           FAVLPEYEQFLTKFFKEVAE KKKKKVEEDDSAIEDGKAKPRCCCFQ
Sbjct: 301 FAVLPEYEQFLTKFFKEVAE-KKKKKVEEDDSAIEDGKAKPRCCCFQ 346

BLAST of CmoCh20G009130 vs. NCBI nr
Match: KAG7010978.1 (Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 639.0 bits (1647), Expect = 2.3e-179
Identity = 329/332 (99.10%), Postives = 330/332 (99.40%), Query Frame = 0

Query: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60
           MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI
Sbjct: 1   MASSSSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCI 60

Query: 61  IHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120
           IHKLYDDAWAEHLQDFA KNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP
Sbjct: 61  IHKLYDDAWAEHLQDFASKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVP 120

Query: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180
           KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE
Sbjct: 121 KQIVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQE 180

Query: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240
           FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN
Sbjct: 181 FVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCN 240

Query: 241 GAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAVL 300
           GAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFD IRDGNGTYYVIDINYLPGFAVL
Sbjct: 241 GAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDTIRDGNGTYYVIDINYLPGFAVL 300

Query: 301 PEYEQFLTKFFKEVAEKKKKKKVEEDDSAIED 333
           PEYEQFLTKFFKEVAEKKKK+KVEEDDSAIED
Sbjct: 301 PEYEQFLTKFFKEVAEKKKKEKVEEDDSAIED 332

BLAST of CmoCh20G009130 vs. TAIR 10
Match: AT5G16760.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 272.3 bits (695), Expect = 5.2e-73
Identity = 148/319 (46.39%), Postives = 211/319 (66.14%), Query Frame = 0

Query: 5   SSSSSNRLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIHKL 64
           S S   R  VGYA    K+ + I+PSL+++++  G+DLV++D    LL QG   CIIHKL
Sbjct: 2   SDSIQERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKL 61

Query: 65  YDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVK--ASDSGVRIEVPKQ 124
           YD  W E+L +F  K P V V+D P+ I RL++RVSML+V++Q++   SDS  R  VP+Q
Sbjct: 62  YDVYWKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDS-ERFGVPEQ 121

Query: 125 IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFV 184
           +V+             ++KFPVIAKPL +DGSAKSH+M L++++ G+K L  P++LQEFV
Sbjct: 122 VVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFV 181

Query: 185 NHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEK-CNG 244
           NHGGV+FKVYV G+   CVKR+SLPD++E +    K   G++ FSQIS    + +K    
Sbjct: 182 NHGGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSK---GSLPFSQISNLTAQEDKNIEY 241

Query: 245 AEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGN--GTYYVIDINYLPGFAV 304
            E +  E++EMPP   + +++  +RE+MG+ LFNFD+IRD      Y +IDINY PG+A 
Sbjct: 242 GEDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAK 301

Query: 305 LPEYEQFLTKFFKEVAEKK 319
           +P YE  LT+FF ++  KK
Sbjct: 302 MPSYEPVLTEFFWDMVTKK 316

BLAST of CmoCh20G009130 vs. TAIR 10
Match: AT4G33770.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 245.7 bits (626), Expect = 5.2e-65
Identity = 132/315 (41.90%), Postives = 197/315 (62.54%), Query Frame = 0

Query: 11  RLRVGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWA 70
           +L VGYA    K+++ ++P L   A+  G+  V IDL  PL  QGPF  ++HKL    W 
Sbjct: 80  KLVVGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWE 139

Query: 71  EHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQ-IVLKQED 130
           E ++D+  K+P+V V+D P  I R+Y+R SML  ++ +K SD    + VPKQ +VLK   
Sbjct: 140 EVIEDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVLKDSA 199

Query: 131 GEIDSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFVNHGGVMF 190
              D++ +  +KFP++AKPL  DG+AKSH++ L ++RR L +LD P++LQEFVNHGGVMF
Sbjct: 200 ASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMF 259

Query: 191 KVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGAEKKEAEE 250
           KV+V G+    ++R SLP+V+  E   + K DG  +F ++S A   ++  +     +   
Sbjct: 260 KVFVVGDVIKVMRRFSLPNVSNCE---KAKVDGVFQFPRVSSAAASADNAD----LDPRV 319

Query: 251 IEMPPEKIVREVSNGLREAMGIRLFNFDMIRD--GNGTYYVIDINYLPGFAVLPEYEQFL 310
            E+PP+  +  +   LR  +G+RLFN DMIR+      +YVIDINY PG+  LP+YEQ  
Sbjct: 320 AELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDYEQVF 379

Query: 311 TKFFKEVAEKKKKKK 323
             FF+ +A+ K KK+
Sbjct: 380 VDFFQNLAQVKYKKR 387

BLAST of CmoCh20G009130 vs. TAIR 10
Match: AT4G08170.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 235.7 bits (600), Expect = 5.4e-62
Identity = 130/317 (41.01%), Postives = 193/317 (60.88%), Query Frame = 0

Query: 14  VGYAFPPNKERNVIRPSLVDYAKLHGVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWAEHL 73
           VGYA    K ++ ++P L   A+  G+  V ID   PL  QGPF  ++HK     W   L
Sbjct: 43  VGYALTSKKIKSFLQPKLEGLARNKGILFVAIDQNKPLSEQGPFDIVLHKQIGKEWRRIL 102

Query: 74  QDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQIVLKQEDGEI- 133
           ++F L +PDV V+D PD I  L +R SML  V+ +  SDS  R+ VPKQ+V+K++   I 
Sbjct: 103 EEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKDASSIP 162

Query: 134 DSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFVNHGGVMFKVY 193
           +++ +  ++ P++AKPL +DGSAKSHE+SL +++  L  L+ P++LQEFVNHGGV+FKVY
Sbjct: 163 EAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGVLFKVY 222

Query: 194 VAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGAEKKEAEEIEM 253
           + GE    V+R SLPDV+  E     K  G  +F ++S A   ++  +     +    E+
Sbjct: 223 IVGEAIRVVRRFSLPDVSRRE---LPKSAGVFRFPRVSCAAASADDAD----LDPSIAEL 282

Query: 254 PPEKIVREVSNGLREAMGIRLFNFDMIRDGNGT---YYVIDINYLPGFAVLPEYEQFLTK 313
           PP  ++  ++  LR  +G+RLFN D+IR+ +GT   +YVIDINY PG+  +PEYE   T 
Sbjct: 283 PPRPLLERLAKELRRGLGLRLFNLDIIRE-HGTRDRFYVIDINYFPGYGKMPEYEHVFTD 342

Query: 314 FFKEVAEKKKKKKVEED 327
           F   V + + KK+   D
Sbjct: 343 FLLSVVQSQCKKRALAD 351

BLAST of CmoCh20G009130 vs. TAIR 10
Match: AT4G33770.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.9e-60
Identity = 123/287 (42.86%), Postives = 181/287 (63.07%), Query Frame = 0

Query: 39  GVDLVRIDLQTPLLHQGPFHCIIHKLYDDAWAEHLQDFALKNPDVVVVDRPDLIARLYDR 98
           G+  V IDL  PL  QGPF  ++HKL    W E ++D+  K+P+V V+D P  I R+Y+R
Sbjct: 15  GIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWEEVIEDYQQKHPEVTVLDPPGSIQRIYNR 74

Query: 99  VSMLDVVSQVKASDSGVRIEVPKQ-IVLKQEDGEIDSIRDLDVKFPVIAKPLASDGSAKS 158
            SML  ++ +K SD    + VPKQ +VLK      D++ +  +KFP++AKPL  DG+AKS
Sbjct: 75  QSMLQGMADLKLSDCSGSLFVPKQMVVLKDSAASADAVVEAGLKFPLVAKPLWIDGTAKS 134

Query: 159 HEMSLVFNRRGLKDLDRPVLLQEFVNHGGVMFKVYVAGEESVCVKRKSLPDVAETEEELE 218
           H++ L ++RR L +LD P++LQEFVNHGGVMFKV+V G+    ++R SLP+V+  E   +
Sbjct: 135 HQLYLAYDRRSLAELDPPLVLQEFVNHGGVMFKVFVVGDVIKVMRRFSLPNVSNCE---K 194

Query: 219 KKQDGAMKFSQISRAEEKSEKCNGAEKKEAEEIEMPPEKIVREVSNGLREAMGIRLFNFD 278
            K DG  +F ++S A   ++  +     +    E+PP+  +  +   LR  +G+RLFN D
Sbjct: 195 AKVDGVFQFPRVSSAAASADNAD----LDPRVAELPPKPFLEALVKELRSLLGLRLFNID 254

Query: 279 MIRD--GNGTYYVIDINYLPGFAVLPEYEQFLTKFFKEVAEKKKKKK 323
           MIR+      +YVIDINY PG+  LP+YEQ    FF+ +A+ K KK+
Sbjct: 255 MIREHGSKNVFYVIDINYFPGYGKLPDYEQVFVDFFQNLAQVKYKKR 294

BLAST of CmoCh20G009130 vs. TAIR 10
Match: AT4G08170.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.3e-50
Identity = 110/262 (41.98%), Postives = 165/262 (62.98%), Query Frame = 0

Query: 69  WAEHLQDFALKNPDVVVVDRPDLIARLYDRVSMLDVVSQVKASDSGVRIEVPKQIVLKQE 128
           W   L++F L +PDV V+D PD I  L +R SML  V+ +  SDS  R+ VPKQ+V+K++
Sbjct: 10  WRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKD 69

Query: 129 DGEI-DSIRDLDVKFPVIAKPLASDGSAKSHEMSLVFNRRGLKDLDRPVLLQEFVNHGGV 188
              I +++ +  ++ P++AKPL +DGSAKSHE+SL +++  L  L+ P++LQEFVNHGGV
Sbjct: 70  ASSIPEAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGV 129

Query: 189 MFKVYVAGEESVCVKRKSLPDVAETEEELEKKQDGAMKFSQISRAEEKSEKCNGAEKKEA 248
           +FKVY+ GE    V+R SLPDV+  E     K  G  +F ++S A   ++  +     + 
Sbjct: 130 LFKVYIVGEAIRVVRRFSLPDVSRRE---LPKSAGVFRFPRVSCAAASADDAD----LDP 189

Query: 249 EEIEMPPEKIVREVSNGLREAMGIRLFNFDMIRDGNGT---YYVIDINYLPGFAVLPEYE 308
              E+PP  ++  ++  LR  +G+RLFN D+IR+ +GT   +YVIDINY PG+  +PEYE
Sbjct: 190 SIAELPPRPLLERLAKELRRGLGLRLFNLDIIRE-HGTRDRFYVIDINYFPGYGKMPEYE 249

Query: 309 QFLTKFFKEVAEKKKKKKVEED 327
              T F   V + + KK+   D
Sbjct: 250 HVFTDFLLSVVQSQCKKRALAD 263

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SBA57.4e-7246.39Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 P... [more]
Q84Y011.2e-6945.78Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1[more]
A0JJZ63.2e-6745.19Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q7XBW03.2e-6745.19Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
O818937.4e-6441.90Inositol-tetrakisphosphate 1-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ITPK2 P... [more]
Match NameE-valueIdentityDescription
A0A6J1FTQ52.0e-189100.00Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC11144836... [more]
A0A6J1JBH85.0e-18095.39Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111484264 ... [more]
A0A5A7TY892.2e-15985.38Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3C9X32.2e-15985.38Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo OX=3656 GN=LOC103498455 PE=3... [more]
A0A6J1G5K03.5e-15784.30Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC11145108... [more]
Match NameE-valueIdentityDescription
XP_022943687.14.2e-189100.00inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita moschata][more]
KAG6571172.15.1e-18799.12Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023512946.17.6e-18396.82inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022986561.11.0e-17995.39inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima][more]
KAG7010978.12.3e-17999.10Inositol-tetrakisphosphate 1-kinase 1, partial [Cucurbita argyrosperma subsp. ar... [more]
Match NameE-valueIdentityDescription
AT5G16760.15.2e-7346.39Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.15.2e-6541.90Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.25.4e-6241.01Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.23.9e-6042.86Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.11.3e-5041.98Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.1490.220coord: 122..222
e-value: 1.7E-29
score: 103.5
NoneNo IPR availableGENE3D3.40.50.11370coord: 2..115
e-value: 8.6E-23
score: 82.5
NoneNo IPR availableGENE3D3.30.470.20coord: 223..321
e-value: 9.2E-7
score: 30.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..248
NoneNo IPR availablePANTHERPTHR14217:SF17INOSITOL-TETRAKISPHOSPHATE 1-KINASEcoord: 9..332
NoneNo IPR availableSUPERFAMILY56059Glutathione synthetase ATP-binding domain-likecoord: 88..310
IPR008656Inositol-tetrakisphosphate 1-kinasePIRSFPIRSF038186ITPKcoord: 1..327
e-value: 6.8E-108
score: 358.5
IPR008656Inositol-tetrakisphosphate 1-kinasePANTHERPTHR14217INOSITOL-TETRAKISPHOSPHATE 1-KINASEcoord: 9..332
IPR040464Inositol 1,3,4-trisphosphate 5/6-kinase, ATP-grasp domainPFAMPF05770Ins134_P3_kincoord: 115..312
e-value: 1.3E-67
score: 227.2
IPR041429Inositol-tetrakisphosphate 1-kinase, N-terminalPFAMPF17927Ins134_P3_kin_Ncoord: 13..92
e-value: 1.5E-23
score: 83.0
IPR011761ATP-grasp foldPROSITEPS50975ATP_GRASPcoord: 109..320
score: 15.100093

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G009130.1CmoCh20G009130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052746 inositol phosphorylation
biological_process GO:0032957 inositol trisphosphate metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0052726 inositol-1,3,4-trisphosphate 5-kinase activity
molecular_function GO:0052725 inositol-1,3,4-trisphosphate 6-kinase activity
molecular_function GO:0047325 inositol tetrakisphosphate 1-kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0046872 metal ion binding