CmoCh20G009010 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G009010
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCmo_Chr20: 4574894 .. 4575859 (+)
RNA-Seq ExpressionCmoCh20G009010
SyntenyCmoCh20G009010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCACCGAAATTGCCCACAACTTTTCCCCTTTGTTTATCGTTTACAAAGACGGTACCGTCCACAGGTTAGTCGGCAACGATGCAATACCCCCTTCTCCGGATCCCGTTACCGGCGTCGATTCCAAAGACGTCGTCATCTCCCCGGAAACCGCTGTCTCTGTTCGGATTTACAGGCCCAAACCCGCCACCGCACAACCCCAGAAGCTTCCTGTTCTCGTTTACGTCCACGGCGGTGGCTTCTGCATCGAATCGGCATTCTCCCCTTTCTACGACCGCCACGTCAAGTCCTTAGCTGCTGAAGCCAACGTTGTCGCCGTCTCCGTCGACTACCGCCTTGCTCCGGAGCACCCTCTTCCGATTGCTTATGAAGATTCATGGGCCGCTCTGAAATGGGTTGCTGCCCATTCCGACGGAAACGGGCCGGAGGAGTGGCTGAATGGAATCGTGGATTTGGATCGGGTTTATTTTGCTGGAGATAGCGCCGGAGCTAATATTGTTCATCACTTGGCGGTTCGGGTCGGGTCGGAGGGGATAGCCGGGTTGAATCTGAAGGGATTGATTTTGGTTCATCCTTATTTCTGGGGAGGGAAATTGGTCGGAGATGAAGAGAAGCTGAGACCAGAGGAAAGACACTTTATGGAGAAATTGTGGTATGTGACTTGCCCTACAATTAGCGGGTTAGATGACCCGATTGTGAACCCGGAATATGACCCGAAATTGGGACAGCTGGCGGCGGAGAGAGTGGTGGTTTATGTGGCGGAGAAGGATGCTCTGAAGAACAGAGGATGGTTTTACAGTGAGTGTTTGAAGAAATCGGGATGGGGTGGCACCGTCGAGGTCGTGGAGACAAAAGGCGAAGGCCACGTGTTTCATTTGTTCAACCCCACGACCGACGTGGCGCTGGATTTCGTCAGAAACCTTGCTGCCTTCATCAATGGCAATGGCGGCCGCCGTGAGTGA

mRNA sequence

ATGGATTCCACCGAAATTGCCCACAACTTTTCCCCTTTGTTTATCGTTTACAAAGACGGTACCGTCCACAGGTTAGTCGGCAACGATGCAATACCCCCTTCTCCGGATCCCGTTACCGGCGTCGATTCCAAAGACGTCGTCATCTCCCCGGAAACCGCTGTCTCTGTTCGGATTTACAGGCCCAAACCCGCCACCGCACAACCCCAGAAGCTTCCTGTTCTCGTTTACGTCCACGGCGGTGGCTTCTGCATCGAATCGGCATTCTCCCCTTTCTACGACCGCCACGTCAAGTCCTTAGCTGCTGAAGCCAACGTTGTCGCCGTCTCCGTCGACTACCGCCTTGCTCCGGAGCACCCTCTTCCGATTGCTTATGAAGATTCATGGGCCGCTCTGAAATGGGTTGCTGCCCATTCCGACGGAAACGGGCCGGAGGAGTGGCTGAATGGAATCGTGGATTTGGATCGGGTTTATTTTGCTGGAGATAGCGCCGGAGCTAATATTGTTCATCACTTGGCGGTTCGGGTCGGGTCGGAGGGGATAGCCGGGTTGAATCTGAAGGGATTGATTTTGGTTCATCCTTATTTCTGGGGAGGGAAATTGGTCGGAGATGAAGAGAAGCTGAGACCAGAGGAAAGACACTTTATGGAGAAATTGTGGTATGTGACTTGCCCTACAATTAGCGGGTTAGATGACCCGATTGTGAACCCGGAATATGACCCGAAATTGGGACAGCTGGCGGCGGAGAGAGTGGTGGTTTATGTGGCGGAGAAGGATGCTCTGAAGAACAGAGGATGGTTTTACAGTGAGTGTTTGAAGAAATCGGGATGGGGTGGCACCGTCGAGGTCGTGGAGACAAAAGGCGAAGGCCACGTGTTTCATTTGTTCAACCCCACGACCGACGTGGCGCTGGATTTCGTCAGAAACCTTGCTGCCTTCATCAATGGCAATGGCGGCCGCCGTGAGTGA

Coding sequence (CDS)

ATGGATTCCACCGAAATTGCCCACAACTTTTCCCCTTTGTTTATCGTTTACAAAGACGGTACCGTCCACAGGTTAGTCGGCAACGATGCAATACCCCCTTCTCCGGATCCCGTTACCGGCGTCGATTCCAAAGACGTCGTCATCTCCCCGGAAACCGCTGTCTCTGTTCGGATTTACAGGCCCAAACCCGCCACCGCACAACCCCAGAAGCTTCCTGTTCTCGTTTACGTCCACGGCGGTGGCTTCTGCATCGAATCGGCATTCTCCCCTTTCTACGACCGCCACGTCAAGTCCTTAGCTGCTGAAGCCAACGTTGTCGCCGTCTCCGTCGACTACCGCCTTGCTCCGGAGCACCCTCTTCCGATTGCTTATGAAGATTCATGGGCCGCTCTGAAATGGGTTGCTGCCCATTCCGACGGAAACGGGCCGGAGGAGTGGCTGAATGGAATCGTGGATTTGGATCGGGTTTATTTTGCTGGAGATAGCGCCGGAGCTAATATTGTTCATCACTTGGCGGTTCGGGTCGGGTCGGAGGGGATAGCCGGGTTGAATCTGAAGGGATTGATTTTGGTTCATCCTTATTTCTGGGGAGGGAAATTGGTCGGAGATGAAGAGAAGCTGAGACCAGAGGAAAGACACTTTATGGAGAAATTGTGGTATGTGACTTGCCCTACAATTAGCGGGTTAGATGACCCGATTGTGAACCCGGAATATGACCCGAAATTGGGACAGCTGGCGGCGGAGAGAGTGGTGGTTTATGTGGCGGAGAAGGATGCTCTGAAGAACAGAGGATGGTTTTACAGTGAGTGTTTGAAGAAATCGGGATGGGGTGGCACCGTCGAGGTCGTGGAGACAAAAGGCGAAGGCCACGTGTTTCATTTGTTCAACCCCACGACCGACGTGGCGCTGGATTTCGTCAGAAACCTTGCTGCCTTCATCAATGGCAATGGCGGCCGCCGTGAGTGA

Protein sequence

MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYRPKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGIAGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTDVALDFVRNLAAFINGNGGRRE
Homology
BLAST of CmoCh20G009010 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 5.1e-83
Identity = 162/329 (49.24%), Postives = 214/329 (65.05%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS EIA ++SP+ I+YK G + RLVG   +PPS +P  GV SKDVV SP+  +S+RIY 
Sbjct: 1   MDS-EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYL 60

Query: 61  P-KPATAQPQ---KLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAP 120
           P K ATA+ +   KLP+LVY HGGGF +E+AFSP Y   + +  + ++ VAVSVDYR AP
Sbjct: 61  PEKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAP 120

Query: 121 EHPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVG 180
           EHP+P +Y+DSW ALKWV +H  G+G E+WLN   D  +V+ AGDSAGANI HH+ ++  
Sbjct: 121 EHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAA 180

Query: 181 SEGIA--GLN---LKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCP-TISGLD 240
            + ++   LN   + G+ILVHPYFW    V D+E      R ++E +W +  P +  G D
Sbjct: 181 KDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSD 240

Query: 241 DPIVN--PEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTV-EVVETKG 300
           DP +N        L  L   +V+V VAEKDAL  +GW Y E L KS W G V +VVETKG
Sbjct: 241 DPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKG 300

Query: 301 EGHVFHLFNPTTDVALDFVRNLAAFINGN 317
           EGHVFHL +P ++ A + V   A FI G+
Sbjct: 301 EGHVFHLRDPNSEKAHELVHRFAGFIKGD 328

BLAST of CmoCh20G009010 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 4.3e-82
Identity = 157/323 (48.61%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS EIA + SPL  +YK G + RL+G   +PPS +P  GV SKDVV S +  +SVRIY 
Sbjct: 1   MDS-EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYL 60

Query: 61  PKPATAQ-PQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHP 120
           P+ A A+   KLP+LVY HGGGF IE+AFSP Y   + +  + +N VAVSVDYR APEHP
Sbjct: 61  PEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHP 120

Query: 121 LPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEG 180
           + + ++DSW ALKWV  H  G+G E+WLN   D  RV+ +GDSAGANIVHH+A+R   E 
Sbjct: 121 ISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEK 180

Query: 181 IA-GLN---LKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCP-TISGLDDPIV 240
           ++ GLN   + G+IL+HPYFW    + +++      R  +E  W +  P +  G DDP++
Sbjct: 181 LSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLL 240

Query: 241 N--PEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVF 300
           N        L  L   +V+V VAEKDAL  +GW Y+  L+KSGW G VEVVE++GE HVF
Sbjct: 241 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVF 300

Query: 301 HLFNPTTDVALDFVRNLAAFING 316
           HL  P  D A++ +   + FI G
Sbjct: 301 HLLKPECDNAIEVMHKFSGFIKG 322

BLAST of CmoCh20G009010 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.4e-80
Identity = 155/319 (48.59%), Postives = 202/319 (63.32%), Query Frame = 0

Query: 1   MDST--EIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRI 60
           M+ST  +++    P  +V+ DGTV RL G +  PP  DP+TGV SKD++I P+T +S RI
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  YRPKPATAQP-QKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPE 120
           YR  P + QP QK+P+++Y HGG F I S   P Y   +  +  +ANV+AVSV+YRLAPE
Sbjct: 61  YR--PFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPE 120

Query: 121 HPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGS 180
           HPLP AYEDSW ALK + A +     E W+N   DLD ++  GDSAGANI HHLA R   
Sbjct: 121 HPLPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AK 180

Query: 181 EGIAGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPE 240
           +    L +KG+ ++HPYFWG + +G E K     +  ++  W   CP+  G DDP +NP 
Sbjct: 181 QSDQTLKIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPF 240

Query: 241 YD--PKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLF 300
            D  P LG L  ERV++ VAEKD L  RG  Y E L KS W G VE++ETK + HVFH+F
Sbjct: 241 ADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIF 300

Query: 301 NPTTDVALDFVRNLAAFIN 315
            P  D A++ VR LA FIN
Sbjct: 301 EPDCDEAMEMVRCLALFIN 310

BLAST of CmoCh20G009010 vs. ExPASy Swiss-Prot
Match: Q9ZQ91 (Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.3e-80
Identity = 155/315 (49.21%), Postives = 196/315 (62.22%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS  IA + SP+F VYK G + RL+G   +PPS  P  GV SKD++ SPE  +S+RIY 
Sbjct: 1   MDSV-IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYL 60

Query: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120
           P+  T   +KLP+L+Y HGGGF IE+AFSP Y   + S  A AN +A+SV+YR APE P+
Sbjct: 61  PEKVTV--KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPV 120

Query: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180
           PI YEDSW +LKWV  H  G GPE W+N   D  +V+ AGDSAG NI HHL +R   E +
Sbjct: 121 PIPYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKL 180

Query: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCP-TISGLDDPIVNPEYD 240
               + G+IL+HPYFW    + + E     +   +E  W V  P +  G+DDP +N    
Sbjct: 181 CDSLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGS 240

Query: 241 PKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTT 300
              G L   RV+V VA  D    +GW Y+E LKKSGW G VEV+ETK EGHVFHL NP +
Sbjct: 241 DPSG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNS 300

Query: 301 DVALDFVRNLAAFIN 315
           D A   V+ L  FIN
Sbjct: 301 DNARQVVKKLEEFIN 311

BLAST of CmoCh20G009010 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.1e-76
Identity = 143/317 (45.11%), Postives = 196/317 (61.83%), Query Frame = 0

Query: 4   TEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYRPKP 63
           +EIA  F P   +YKDG V RL+G D IP S DP   V SKDV+ SPE  +SVR++ P  
Sbjct: 3   SEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHK 62

Query: 64  AT--AQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPLP 123
           +T      KLP+L+Y+HGG + IES FSP Y  ++  +   AN +AVSV YR APE P+P
Sbjct: 63  STKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 122

Query: 124 IAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGIA 183
            AYED W+A++W+ AHS+G+GP +W+N   D  +V+  GDSAG NI HH+A++ G E   
Sbjct: 123 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 182

Query: 184 GLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLW-YVTCP-TISGLDDPIVNPE-Y 243
            L +KG+ +VHP FWG   V + +    E R  + ++W  +  P +++G DDP+ N    
Sbjct: 183 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 242

Query: 244 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 303
                 L  ++V+V VA KD    +G  Y+  L+K  W GTVEVVE +GE HVFHL NP 
Sbjct: 243 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 302

Query: 304 TDVALDFVRNLAAFING 316
           +D AL F++    FI G
Sbjct: 303 SDKALKFLKKFVEFIIG 319

BLAST of CmoCh20G009010 vs. ExPASy TrEMBL
Match: A0A6J1FW98 (probable carboxylesterase 13 OS=Cucurbita moschata OX=3662 GN=LOC111449075 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 2.9e-190
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120
           PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL
Sbjct: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120

Query: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180
           PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI
Sbjct: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180

Query: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240
           AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP
Sbjct: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240

Query: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300
           KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD
Sbjct: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300

Query: 301 VALDFVRNLAAFINGNGGRRE 322
           VALDFVRNLAAFINGNGGRRE
Sbjct: 301 VALDFVRNLAAFINGNGGRRE 321

BLAST of CmoCh20G009010 vs. ExPASy TrEMBL
Match: A0A6J1JBI9 (probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484271 PE=4 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 2.6e-183
Identity = 310/323 (95.98%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHR +GND IPPSPDP+TGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRFLGNDVIPPSPDPLTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKP--ATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEH 120
           PKP  ATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSL+AEANVVAVSVDYRLAPEH
Sbjct: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLSAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GIAGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           GI GLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDD IVNPEY
Sbjct: 181 GIPGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDSIVNPEY 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 TDVALDFVRNLAAFINGNGGRRE 322
           TDVA+DFVR LAAFINGNGGR +
Sbjct: 301 TDVAVDFVRKLAAFINGNGGRHD 323

BLAST of CmoCh20G009010 vs. ExPASy TrEMBL
Match: A0A5A7SPY3 (Putative carboxylesterase 13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00320 PE=4 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.8e-147
Identity = 244/315 (77.46%), Postives = 278/315 (88.25%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           M STEIAHNFSP+ IVYKDG   RLVGN+ I PS DP+TGV+SKD++ISPET VS RIYR
Sbjct: 1   MASTEIAHNFSPMIIVYKDGRTERLVGNELIDPSLDPLTGVESKDIIISPETPVSARIYR 60

Query: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120
           PKP TA+P KLP+L+Y HGGGFCIESAFSP Y  H+ SL AEANV+A+SV+YR APE+PL
Sbjct: 61  PKP-TAEPHKLPLLIYFHGGGFCIESAFSPTYHNHLNSLVAEANVIAISVEYRRAPEYPL 120

Query: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180
           PIAYED W ALKWVAAHS G GPEEWLN I DL+RVYFAGDSAGANI + +A+RVG EG+
Sbjct: 121 PIAYEDCWTALKWVAAHSAGTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGIEGV 180

Query: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240
           AGLNLKGL+L+HPYFWG KL+GDEEKL+PE+R F+EKLWY+ CPTISGLDDP+VNPE++P
Sbjct: 181 AGLNLKGLMLIHPYFWGEKLIGDEEKLKPEDRWFIEKLWYLACPTISGLDDPVVNPEFEP 240

Query: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300
           KLG LAAERV VYVAEKD+LK+RGWFYSECLKKSGWGG VEVVETKG+GHVFHLFNPT+D
Sbjct: 241 KLGGLAAERVAVYVAEKDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNPTSD 300

Query: 301 VALDFVRNLAAFING 316
            A+DFV  LAAFING
Sbjct: 301 DAVDFVGKLAAFING 314

BLAST of CmoCh20G009010 vs. ExPASy TrEMBL
Match: A0A1S3CAV3 (probable carboxylesterase 13 OS=Cucumis melo OX=3656 GN=LOC103498400 PE=4 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.8e-147
Identity = 244/315 (77.46%), Postives = 278/315 (88.25%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           M STEIAHNFSP+ IVYKDG   RLVGN+ I PS DP+TGV+SKD++ISPET VS RIYR
Sbjct: 1   MASTEIAHNFSPMIIVYKDGRTERLVGNELIDPSLDPLTGVESKDIIISPETPVSARIYR 60

Query: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120
           PKP TA+P KLP+L+Y HGGGFCIESAFSP Y  H+ SL AEANV+A+SV+YR APE+PL
Sbjct: 61  PKP-TAEPHKLPLLIYFHGGGFCIESAFSPTYHNHLNSLVAEANVIAISVEYRRAPEYPL 120

Query: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180
           PIAYED W ALKWVAAHS G GPEEWLN I DL+RVYFAGDSAGANI + +A+RVG EG+
Sbjct: 121 PIAYEDCWTALKWVAAHSAGTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGIEGV 180

Query: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240
           AGLNLKGL+L+HPYFWG KL+GDEEKL+PE+R F+EKLWY+ CPTISGLDDP+VNPE++P
Sbjct: 181 AGLNLKGLMLIHPYFWGEKLIGDEEKLKPEDRWFIEKLWYLACPTISGLDDPVVNPEFEP 240

Query: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300
           KLG LAAERV VYVAEKD+LK+RGWFYSECLKKSGWGG VEVVETKG+GHVFHLFNPT+D
Sbjct: 241 KLGGLAAERVAVYVAEKDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNPTSD 300

Query: 301 VALDFVRNLAAFING 316
            A+DFV  LAAFING
Sbjct: 301 DAVDFVGKLAAFING 314

BLAST of CmoCh20G009010 vs. ExPASy TrEMBL
Match: A0A0A0LIH8 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234520 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.0e-143
Identity = 239/315 (75.87%), Postives = 273/315 (86.67%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           M STEIA+N SP+ IVYKDG   RLVGN+ + PS DP+T V+SKD+VISPET VS RIYR
Sbjct: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60

Query: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120
           PKP TA+P KLP+L+Y+HGGGFCIESAFSP Y  H+ SL AEANV+A+SV+YR APEHPL
Sbjct: 61  PKP-TAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPL 120

Query: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180
           PIAYEDSW ALKWVAAHS G GPEEWLN I D +RVYFAGDSAGAN+ + +A+RVG EG+
Sbjct: 121 PIAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGV 180

Query: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240
           AGLNLKGL+LVHPYFWG KL+GDEEKL+PEER F+EKLWYV CPTISGLDDPIVNPE++P
Sbjct: 181 AGLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEP 240

Query: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300
            LG++ AERV VYVAEKDALK+RG FYSECLKKSGWGG VEV ETKG+GHVFHLFNPT+D
Sbjct: 241 NLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNPTSD 300

Query: 301 VALDFVRNLAAFING 316
            A+ FV  LAAF+NG
Sbjct: 301 DAVQFVGKLAAFLNG 314

BLAST of CmoCh20G009010 vs. NCBI nr
Match: XP_022944692.1 (probable carboxylesterase 13 [Cucurbita moschata])

HSP 1 Score: 674.1 bits (1738), Expect = 6.0e-190
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120
           PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL
Sbjct: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120

Query: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180
           PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI
Sbjct: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180

Query: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240
           AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP
Sbjct: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240

Query: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300
           KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD
Sbjct: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300

Query: 301 VALDFVRNLAAFINGNGGRRE 322
           VALDFVRNLAAFINGNGGRRE
Sbjct: 301 VALDFVRNLAAFINGNGGRRE 321

BLAST of CmoCh20G009010 vs. NCBI nr
Match: KAG6571159.1 (putative carboxylesterase 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 666.4 bits (1718), Expect = 1.3e-187
Identity = 320/327 (97.86%), Postives = 321/327 (98.17%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHRL+GNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120
           PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL
Sbjct: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120

Query: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180
           PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI
Sbjct: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180

Query: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240
           AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP
Sbjct: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240

Query: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300
           KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD
Sbjct: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300

Query: 301 VALDFVRNLAAFI------NGNGGRRE 322
           VALDFVRNLAAFI      NGNGGRRE
Sbjct: 301 VALDFVRNLAAFINGNGNGNGNGGRRE 327

BLAST of CmoCh20G009010 vs. NCBI nr
Match: XP_023512696.1 (probable carboxylesterase 12 [Cucurbita pepo subsp. pepo] >XP_023522494.1 probable carboxylesterase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.4 bits (1700), Expect = 1.5e-185
Identity = 316/323 (97.83%), Postives = 317/323 (98.14%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHRL+GNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKP--ATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEH 120
           PKP  ATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSL AEANVVAVSVDYRLAPEH
Sbjct: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GIAGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           GI GLNLKGLILVHPYFWGGKLVGDEEKLR EERHFMEKLWYVTCPTISGLDDPIVNPEY
Sbjct: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 TDVALDFVRNLAAFINGNGGRRE 322
           TDVALDFVR LAAFINGNGGRRE
Sbjct: 301 TDVALDFVRKLAAFINGNGGRRE 323

BLAST of CmoCh20G009010 vs. NCBI nr
Match: XP_022986571.1 (probable carboxylesterase 12 [Cucurbita maxima])

HSP 1 Score: 651.0 bits (1678), Expect = 5.4e-183
Identity = 310/323 (95.98%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHR +GND IPPSPDP+TGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRFLGNDVIPPSPDPLTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKP--ATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEH 120
           PKP  ATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSL+AEANVVAVSVDYRLAPEH
Sbjct: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLSAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GIAGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           GI GLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDD IVNPEY
Sbjct: 181 GIPGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDSIVNPEY 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 TDVALDFVRNLAAFINGNGGRRE 322
           TDVA+DFVR LAAFINGNGGR +
Sbjct: 301 TDVAVDFVRKLAAFINGNGGRHD 323

BLAST of CmoCh20G009010 vs. NCBI nr
Match: XP_038902770.1 (probable carboxylesterase 13 [Benincasa hispida])

HSP 1 Score: 543.9 bits (1400), Expect = 9.4e-151
Identity = 254/321 (79.13%), Postives = 283/321 (88.16%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           M STEIAH+FSP+ +VYKDG +HRL GN+ I PS DP+TGV+SKDVVISPET VS RIY+
Sbjct: 1   MASTEIAHDFSPMLVVYKDGRIHRLAGNELIDPSLDPLTGVESKDVVISPETPVSARIYK 60

Query: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120
           PKP TAQP KLP+LVY HGGGFCIESAFS FY RH+ SL AEANVVAVSVDYRLAPEHPL
Sbjct: 61  PKP-TAQPHKLPLLVYFHGGGFCIESAFSSFYHRHLNSLVAEANVVAVSVDYRLAPEHPL 120

Query: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180
           PIAYEDSW ALKWVAAHS G GPEEWLNGI DLDRVYFAGDSAGANI + +A+RVG+EG+
Sbjct: 121 PIAYEDSWDALKWVAAHSAGTGPEEWLNGIADLDRVYFAGDSAGANIANRMAIRVGTEGL 180

Query: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240
           AGLNL+GL+LVH YFWGGKL+GDEEKL PE R F+EKLWY  CP ISGLDDPIVNP +DP
Sbjct: 181 AGLNLRGLLLVHAYFWGGKLIGDEEKLNPEHRSFIEKLWYFVCPKISGLDDPIVNPGFDP 240

Query: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300
           +LG++AAERV V+VAEKDALKNRGWFYSECLKKSGWGG V+V+ETKG+GHVFHLFNPT+D
Sbjct: 241 ELGRVAAERVAVFVAEKDALKNRGWFYSECLKKSGWGGAVDVIETKGQGHVFHLFNPTSD 300

Query: 301 VALDFVRNLAAFINGNGGRRE 322
            A+DFV  LAAFING   RRE
Sbjct: 301 EAVDFVGKLAAFINGE--RRE 318

BLAST of CmoCh20G009010 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 309.3 bits (791), Expect = 3.6e-84
Identity = 162/329 (49.24%), Postives = 214/329 (65.05%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS EIA ++SP+ I+YK G + RLVG   +PPS +P  GV SKDVV SP+  +S+RIY 
Sbjct: 1   MDS-EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYL 60

Query: 61  P-KPATAQPQ---KLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAP 120
           P K ATA+ +   KLP+LVY HGGGF +E+AFSP Y   + +  + ++ VAVSVDYR AP
Sbjct: 61  PEKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAP 120

Query: 121 EHPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVG 180
           EHP+P +Y+DSW ALKWV +H  G+G E+WLN   D  +V+ AGDSAGANI HH+ ++  
Sbjct: 121 EHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAA 180

Query: 181 SEGIA--GLN---LKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCP-TISGLD 240
            + ++   LN   + G+ILVHPYFW    V D+E      R ++E +W +  P +  G D
Sbjct: 181 KDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSD 240

Query: 241 DPIVN--PEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTV-EVVETKG 300
           DP +N        L  L   +V+V VAEKDAL  +GW Y E L KS W G V +VVETKG
Sbjct: 241 DPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKG 300

Query: 301 EGHVFHLFNPTTDVALDFVRNLAAFINGN 317
           EGHVFHL +P ++ A + V   A FI G+
Sbjct: 301 EGHVFHLRDPNSEKAHELVHRFAGFIKGD 328

BLAST of CmoCh20G009010 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.1e-83
Identity = 157/323 (48.61%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS EIA + SPL  +YK G + RL+G   +PPS +P  GV SKDVV S +  +SVRIY 
Sbjct: 1   MDS-EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYL 60

Query: 61  PKPATAQ-PQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHP 120
           P+ A A+   KLP+LVY HGGGF IE+AFSP Y   + +  + +N VAVSVDYR APEHP
Sbjct: 61  PEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHP 120

Query: 121 LPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEG 180
           + + ++DSW ALKWV  H  G+G E+WLN   D  RV+ +GDSAGANIVHH+A+R   E 
Sbjct: 121 ISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEK 180

Query: 181 IA-GLN---LKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCP-TISGLDDPIV 240
           ++ GLN   + G+IL+HPYFW    + +++      R  +E  W +  P +  G DDP++
Sbjct: 181 LSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLL 240

Query: 241 N--PEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVF 300
           N        L  L   +V+V VAEKDAL  +GW Y+  L+KSGW G VEVVE++GE HVF
Sbjct: 241 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVF 300

Query: 301 HLFNPTTDVALDFVRNLAAFING 316
           HL  P  D A++ +   + FI G
Sbjct: 301 HLLKPECDNAIEVMHKFSGFIKG 322

BLAST of CmoCh20G009010 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.7e-81
Identity = 155/319 (48.59%), Postives = 202/319 (63.32%), Query Frame = 0

Query: 1   MDST--EIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRI 60
           M+ST  +++    P  +V+ DGTV RL G +  PP  DP+TGV SKD++I P+T +S RI
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  YRPKPATAQP-QKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPE 120
           YR  P + QP QK+P+++Y HGG F I S   P Y   +  +  +ANV+AVSV+YRLAPE
Sbjct: 61  YR--PFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPE 120

Query: 121 HPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGS 180
           HPLP AYEDSW ALK + A +     E W+N   DLD ++  GDSAGANI HHLA R   
Sbjct: 121 HPLPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AK 180

Query: 181 EGIAGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPE 240
           +    L +KG+ ++HPYFWG + +G E K     +  ++  W   CP+  G DDP +NP 
Sbjct: 181 QSDQTLKIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPF 240

Query: 241 YD--PKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLF 300
            D  P LG L  ERV++ VAEKD L  RG  Y E L KS W G VE++ETK + HVFH+F
Sbjct: 241 ADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIF 300

Query: 301 NPTTDVALDFVRNLAAFIN 315
            P  D A++ VR LA FIN
Sbjct: 301 EPDCDEAMEMVRCLALFIN 310

BLAST of CmoCh20G009010 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 299.3 bits (765), Expect = 3.7e-81
Identity = 155/315 (49.21%), Postives = 196/315 (62.22%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS  IA + SP+F VYK G + RL+G   +PPS  P  GV SKD++ SPE  +S+RIY 
Sbjct: 1   MDSV-IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYL 60

Query: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120
           P+  T   +KLP+L+Y HGGGF IE+AFSP Y   + S  A AN +A+SV+YR APE P+
Sbjct: 61  PEKVTV--KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPV 120

Query: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180
           PI YEDSW +LKWV  H  G GPE W+N   D  +V+ AGDSAG NI HHL +R   E +
Sbjct: 121 PIPYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKL 180

Query: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCP-TISGLDDPIVNPEYD 240
               + G+IL+HPYFW    + + E     +   +E  W V  P +  G+DDP +N    
Sbjct: 181 CDSLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGS 240

Query: 241 PKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTT 300
              G L   RV+V VA  D    +GW Y+E LKKSGW G VEV+ETK EGHVFHL NP +
Sbjct: 241 DPSG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNS 300

Query: 301 DVALDFVRNLAAFIN 315
           D A   V+ L  FIN
Sbjct: 301 DNARQVVKKLEEFIN 311

BLAST of CmoCh20G009010 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 287.3 bits (734), Expect = 1.5e-77
Identity = 143/317 (45.11%), Postives = 196/317 (61.83%), Query Frame = 0

Query: 4   TEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYRPKP 63
           +EIA  F P   +YKDG V RL+G D IP S DP   V SKDV+ SPE  +SVR++ P  
Sbjct: 3   SEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHK 62

Query: 64  AT--AQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPLP 123
           +T      KLP+L+Y+HGG + IES FSP Y  ++  +   AN +AVSV YR APE P+P
Sbjct: 63  STKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 122

Query: 124 IAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGIA 183
            AYED W+A++W+ AHS+G+GP +W+N   D  +V+  GDSAG NI HH+A++ G E   
Sbjct: 123 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 182

Query: 184 GLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLW-YVTCP-TISGLDDPIVNPE-Y 243
            L +KG+ +VHP FWG   V + +    E R  + ++W  +  P +++G DDP+ N    
Sbjct: 183 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 242

Query: 244 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 303
                 L  ++V+V VA KD    +G  Y+  L+K  W GTVEVVE +GE HVFHL NP 
Sbjct: 243 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 302

Query: 304 TDVALDFVRNLAAFING 316
           +D AL F++    FI G
Sbjct: 303 SDKALKFLKKFVEFIIG 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMM95.1e-8349.24Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1[more]
Q9SMN04.3e-8248.61Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1[more]
Q9SX782.4e-8048.59Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
Q9ZQ915.3e-8049.21Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1[more]
Q9FX942.1e-7645.11Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FW982.9e-190100.00probable carboxylesterase 13 OS=Cucurbita moschata OX=3662 GN=LOC111449075 PE=4 ... [more]
A0A6J1JBI92.6e-18395.98probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484271 PE=4 SV... [more]
A0A5A7SPY31.8e-14777.46Putative carboxylesterase 13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CAV31.8e-14777.46probable carboxylesterase 13 OS=Cucumis melo OX=3656 GN=LOC103498400 PE=4 SV=1[more]
A0A0A0LIH82.0e-14375.87Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G2345... [more]
Match NameE-valueIdentityDescription
XP_022944692.16.0e-190100.00probable carboxylesterase 13 [Cucurbita moschata][more]
KAG6571159.11.3e-18797.86putative carboxylesterase 13, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023512696.11.5e-18597.83probable carboxylesterase 12 [Cucurbita pepo subsp. pepo] >XP_023522494.1 probab... [more]
XP_022986571.15.4e-18395.98probable carboxylesterase 12 [Cucurbita maxima][more]
XP_038902770.19.4e-15179.13probable carboxylesterase 13 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G48700.13.6e-8449.24carboxyesterase 13 [more]
AT3G48690.13.1e-8348.61alpha/beta-Hydrolases superfamily protein [more]
AT1G47480.11.7e-8148.59alpha/beta-Hydrolases superfamily protein [more]
AT2G03550.13.7e-8149.21alpha/beta-Hydrolases superfamily protein [more]
AT1G49660.11.5e-7745.11carboxyesterase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 74..293
e-value: 3.1E-50
score: 171.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 9..316
e-value: 2.2E-76
score: 259.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 11..315
NoneNo IPR availablePANTHERPTHR23024:SF458CARBOXYLESTERASE 2-RELATEDcoord: 3..314
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 3..314
IPR033140Lipase, GDXG, putative serine active sitePROSITEPS01174LIPASE_GDXG_SERcoord: 156..168

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G009010.1CmoCh20G009010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity