CmoCh20G008840 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G008840
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionlong chain acyl-CoA synthetase 2
LocationCmo_Chr20: 4461074 .. 4470210 (+)
RNA-Seq ExpressionCmoCh20G008840
SyntenyCmoCh20G008840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAAATTGGTGTAGTTTGCTGAGAATTTAATGGCATTCATCACACCCACCTCAACCATCCTCCCAACCGCCCACCTCTGGTTTAGATCAACTGTAATAAAGCCACTTCCACCGGACTTTTTCAGCAACCCACACCCACAAACAGACCAACCCAATCTTCCGCAGCCGTTTCCTTTCAAATTCCGGCCGTTTTCATGACAATGCCGACGGCCTACACGGTGGCCGTCGAGGATGCGACGCCGGAGAGCGGCGGAAAGCCGTCGGCGGGGCCGGTTTACCGGAGCATCTACGCTAAAGATGGGCTTCTCCAGTTGCTGGCTGGGTTCGAATCTCCATGGCAGTTCTTCAGGTTTGCCTTTTTTTTTTTTTTTTTGGATTTAAATTCTTTAAATGATTTTCCTTCGATTTTTTACGTTAATTAAAACCTGATTTTTTTTTTCCCGAGCTAAGCCATTGAGAACTTTCATAATTTATTTATTAAAAATACACATTATATATATATATATATATATATATATATATTAAAACAAATTATAAAATTAATAAAAATAATTTTGAAAGTTTAATGACATAATTAAAATTTACTTGTATTTTTTATTTTATACTTTAAAAATTACATTTAAATATAATTCAACCATTTAAAAATTAAATGTTTCCTTAAAATTAGGAAAAACTTCAATTTATAATCTAAATTTTGAGTTCGTATTAATTAAAATTTTAAATTAATAATTTTAATTATTGAATCCTAAATTTTTATTAATGAATTAATTTTGACTATTTATTAAAGATTTTTTTTAGCTATCTGCATTTGATTTACGATATTTAAAGTTAAAAAATGATCGGTGAAAGTGCGGCAACCAATATGATCTCGTTTCTTGCAGCGATTCCGTCAAGAGGAATCCTGGAAACCGGATGCTTGGTCGTCGGCGAACCAACGGCGATTCCAAGGTAACAATTTCCGGCGAACTCTGAACTTGAAACGAGACCTAAAAGATTAAATTGAGTTATTGGAACTGATTCCATGAGTTTTGCAGGCGGGTTCCTATGTATGGCTCACATATCAAGAAGCGTACGATGCTGCAATCCGGATGGGTTCTGCTATTAGAAAGTGTGGCGTTAATCCTGTAAGTCCAAGGGATGTTCAATGAAATGTGTGTTCTTCGAAGTTCTAATATTGCCTTAATTTTTGACTGCATCAAGTGGAACTATGTTTTGCCCTATTGTGACTGGTTTCTTTTTTTTGTTTTCTAGGGAGATTGCTGCGGTATATATGGATCCAATTGCCCTGAATGGATAATTTCGATGGAGGTAAGAAATTTTGTGTTTTTGAAGATGTATATGAGGTTGAGATTTAGGGGGAAAGTCACCAATGTGTGAGCTAAATCCGCCAATTCTGTGGGACTGTTGGTTCATTTCCCTTCTTTTCAAGTTTTTGAAGTTATCCTTCATTTGTTTCGAATGTGAAAAACATTATCCATAGATTTCTGCGAAGGAATTTAACTCTTAAAACATCATCGTACAATCATAACTTTATCTTAAAAACATAACAATGGCTTATCTTATGATGTAGCACACGTCTTGGGCTTATCTTATGATGTAGCACACGTCTTGAGCTTCATCTTAAAAACATAACAATGCCTTATCTTTACCTAAAAATGATGTAGCACACGTCTTGAGTATTTCGAAGAATACTCCCTAACTGACCCCACTATAGGGGTCATGAAAATGCAAACACATGTAAGTCATGCTCTAGGGACCTATCGTTAAAACATCCTAGGGGTGGCCTCCAATCTAAATTGGATGTGCAGTACTTCCTTACACACGACCCATACGTGCGAGCATGATTCCTTAGGTGGTTCGCACCCTCTGGACCCATCATAGTCGAGCTGGTTGTCCGTAGCGACGTTCGGAGGTTAGCAAACCCTTGAGTGTCATGTGTGGCATGATCACCATCATCATCATAGCGTACGCATCCGTACTCATCATCGTAAAAGGGGAAGTATCCTGATGCATATGTACACACAGAAATGTATGAGGTCTCTATAGTTTCTCGTCTTTAAAACATCTTTATGATGATGGTTTAAGAGTTAAATTCCGCATAAGAGCTTAGGTTAAGATGACGGTAGTGACTTTAGGTAGCAGAAATCTAGAGTCCTTACAATTGATCTACGAGATATAAATTTGAATTTTATTATTAAATGAACCATAAGCCTTCAATTTTGTGTCGAGTAGATTCATGAATTTAAAAAAAATTCAAAAGTTTATCGACTAAATTTGTAAATTTGAAAGTTCTGACACCAAATAAATAAAAATCCCACAGGATAGAGACTTGTTTTAATTTGAAAATTTCAGAACTTTTTTAATTCGATACCATACTGAAAGTTTAAGTTAATATGTTTGAGCTCTCATCTCATCTCCTTTGTTACCTACAGGCCTGTAATAGCCAAGCTATCACTTACGTACCTCTGTATGATACACTAGGTATGGTTCCTTATTCCTCCTGGCATCTCTTGGACTTTAAGAATAAGAGAATAGTAGCTTAAGCGCCTGCATTGACATTTAAACTGTTGCAGGTGCTAATGCTGTCGAATTCATCATTAACCATGCTGAAGTTTCAATTGCTTTTGTTCAAGAAAACAAACTTGCTTCTGTACGTTTCCATGCTGCTAACCTCTTCGTTCTTACCCTGTATACATAGAATTGCTATTTGTTTAAAATGTTCCATGGACATCTGTTTGGTATTAGTTTGTTTTAAACCGTGAAATGCTAGACACAATAGTGCTTTTGGAATTTGAGTTCTAAGTGCCAAAATATGCACAGAGTTTGTTGTTCTTTTTTTTTTTCTTTTTTGATGAGGTATGCATGTAAGAATTAAAAAATTATATTGTTATGAAGCATGTGATGCTTTACTTAAGATATTGTTAAAAAATATCGTCATCCGACTAATTTGCTCCTTACCTTGTTTTGAATTTCTTCCCATAAAAATGGAAAATTAAAAAAGCCCCAAAAAAAAAAAAAAAAAAAAAAAAACACACCTTGCTGAGATTTTTATAAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCAAAAAAAAAAAAAAAAAAAAAACACACACACCTTGCTGAGATTTTTATAAATATAATTGGTTCTTGCACAAACTCCATTTTGTTCAGTGTGAAAATAGAAACGTATAGCTGCAAATTCTCAATGTCTCCTGGACAACATCTTATATCTTTGTGTTAAAAGTTTGCAACTGGATAGTTACAATGCTGTGGTGTATTTTGAAGGAAGTGAAAACTTTTTAACCGTAAGATGTAAACCGCCATTCTCCTTTCTAGCTATGAATTGGTACTTCTTTTCCAATTTTTGCTTGACATAAAATATTATTCATTGTGCAGATCTTTTCATGCCTTCCCAGGTGTTCGACTCGATTAAAAAGTACGTTGCATTAATTTAGAACGGCACTTTCGTTTTAGAGTTTTGTAAATCTAACCATCGTGTCTTTTTGGTTCAGCTATTGTCAGTTTTACCAATGTTTCAAGCGTACATAAGAAGGAAGCCGAAGAACTGGGAGTATCTTGCTATTCTTGGGAGGAGTTTTATCAGTTGGTACAAAATGTTTTTATCTTGACCAGTTTTTGCCTGATCATTAACCATGGCAGCATATCTAATCTTTGTTCTCTTTGCTCCAGGGAAGCTTGGACTGTGAACTCCCTTCAAAACAGAAGACCAATATCTGCACAATAATGTATACAAGTGGAACCACCGGGGAACCGAAAGGTGTTATCTTAAATAATGCAGCTATAATGGCCGAAGTTTTGTCTGTGGACCACATACTTTTATTGACAGATAAAGTGGTAAGCAGCCAAATATAAATTTTGACCTGGTTAAAGAATAGTTGAGTTCTTTAATTACTATGTTCTTATTAGTTTGATTAATGTTCTATCACATTCTTCTCATATTTTGCATGACTGTAGTAATTACACGTTGGTAAACTTACAGGCGACAGAAGAAGATTCATATTTCTCATTTCTTCCTCTAGCCCATGTGTACGATCAGATAATGGAGACCTATTGCATCTACAAGGGTTCATCTATCGGGTTCTGGCGTGGGGTAATTTTGATCAGTCGATATTCTTGCTTTTATGATCTTACCTCCTAATTATGTGAACTTATGCGGTAGTTTGTGTGTTAACTTTGCAGGACATCAGGTTTTTGCTGGATGATATTCAGGAACTGAAGCCAACAATATTTTCTGGAGTTCCTCGGGTCTATGATCGTATTTATTCTGGTGCATAATATGTTCTTGTTTCGTGTTCTTCATTTAGTTATTATCCAATGTTTTGAAATTACGTGGTTTACGTAATTTCCATAACCTTTGTTGTTATAGGTATTCTGAACAAAGTTTCGTCCGGAGGTAGTTTGCGGAAGATGTTATTCGATTATGCATACAATTAGTACGTTCGAATTCTGTGTTCAATATACTGGTTTAAGTAACCTTTATTATCTCCTATTGTGTAACTGACAAAGAGAGTGCTAACAGCAAGCTAGGATATCTGTCGAAGGGTGTGCCCCAGGAAAAAGCTGCACCTCTCTTGGACAAGCTTGTATTTGACAAGGTTTCTCATTTATTCTGTTTATATATGTTCATTCAAATATCAAGCTTATTTGCATAATATAAGTGCTATTATATTGTGAAAGATAAAAGAAGCATTTGGTGGAAGAGTTCGTCTATTTTTATCTGGTGCTGCACCTTTGCCCAGGCATGTGGAAGAGTTCTTGAGGGTTACCAGTTGTGCAACTTTAAGCCAAGGATATGGTATGCAATATAATATTATCAAACTAGATTTACCCGAATAATTCTCAAGTCATCTGAAAAAATTAGTGCCAGCTATCGGAAAAATTGAGGCAAAAAATTCCGAGTTCGAAGAGATCATAATGCATTAATAATTTTTTTAATGAGCTCGTATCTTATTGCAACTTTAGGCCTTACAGAAAGTTGTGGTGGATGTCTTACATCCATAGCCAATGTTTTCACCATGATGGGAACTGTTGGAGTTCCAGTTACTACCATTGAAGTAAGGCTCGAGTCAGTTCCTGATATGGGGTATGATGCACTGTCAAGCACACCTCGTGGAGAGATTTGCTTGAGGGGAAGTACTTTGTTCTCTGGATATCATAAACGGCAAGATCTAACAGAAGATGTTCTTATTGACGGATGGTTTCATACAGGTAAAACCTCACAGTAATATCTATATTATAATTAGTGGGATTATAAGGGAGAGTTTCTCGACATATTTTACCCTTTTTCACCATCTCCTCATTTTAATTATACGTTTTCATATCCGTTTTGTCCTTGCGTTCCTTTATGGAAATTGTTTCCTCAAAAGAATTAGTTTCTCTTAATTTTTTGACCATGAAAGCTAAACAAATTAGTAAAATTAGGTTGATCCGCTGAGATCTCAAATAAGGTGCGAATATAGCTGGAAGTTCAGTTCAAACACATCTGTCGTAGAGATGTGTTTCATAATGTGTGAGATCCCACATAGATTGAAGAGGGGAACGACATCGATTGGAAAGGAAAACGAGTGTCAACGAGGACGTTGGGCCCCGAAGAGGGGTGAATTGTGAGATCCCACATCGGTTGGGGAGGAGAATGAAGCATTCTTTATAAGGGCATGAAAACGTCTCCCTAGCAGACACGTTTTAAAAACCTTGAGAGGAAGTCCAGAAGGGAAAACCTAAAGAGGACAATATTTGCTAGTGGTGGGCTTGGGCCGTTAAGCTCTCTCTCTCTCTCTCCACCGGACCTTACTTGTGAACATGATCTTTTCTATAGGTTGTACAACCATGTGCTTGTCTAAAACACTTTCTTTCCTCGAGAGAAAAAATGATGCATCTTAATGATTTAAAGAAAGCCAACATCCATAATTATTACTAGGAATAAATAATATTTCAAAGTGCTTTATGCTTCAGCACAATTGACTTGAGCCATTTTCACTCAAGTCAACATTCCAGCCCCTTCTTTTGACTTTCTTGATCTATTATTATACTGGGCCACTAGTGTTGGTCTTCCATTCTTTCTTTTTAATATTCATAAGGTCTATGCTAACTTTTGCTCAAATCAACTAATTTTACTTGATTACTATTTGATGCTACAAATTTTTTTCGTTTTGGTAATTAGGAAACTCGTAGAATTTTAAACCCAAGCCTCCTAAGTTATGCACACTTGTTCTATGTGCTCTTGTGACCACTAAGCCTCCTAAATTATGCACATTTAATTATGTTGCAGGAAAGTCATATGTTAAACTGATTCTTTATACGTGCAGTTCGTTATCTATTTTACGTATAACCTTTTCATCATTATTAAATGTGTTTCAACGCTTTTTTCTAATCATCTGAACATTTGGTTTATGAATGCCATTAGCTATTTACTAGAACAAATGTATACATGTTACAGGGGACATTGGCGAATGGCAGCCTGATGGATCGATGAAAATAATTGATCGGAAAAAGAATATATTTAAGCTGTCCCAGGGGGAGTATGTTGCAGTGGAGAACATTGAAAACATATTCTTGCAATGCCCTCTGATTACCTCGGTACTGCCTACTGGATAGAAATTGATTCTTGCATAACTGAGATACATTAGATGACAGTTTTGTTTTATTTGGATGCTTTCTCAAACATATTGGAATTATGTTTCTTCACTTCTAAAATTTTAATTTTAATCTTTCCATTACTCATTTTTCAAAAAACAATTTCTGTCGCATACAGATTTGGGTCTATGGAAATAGTTTCGAGTCATTTCTTGTGGGTGTGGCGGTTCCCGATCAGAAGGCCTTGGAGGATTGGGCACGGAACCATAATGTGACTGGTGACTTTAAATCTTTGTGTGAGAATCTCAGTGCAAGGAAGTATATTTTGGATGAGCTCAATAGCAATGGTCAGAAGCACCATGTATGTGCCGTTTAGATACTCAATTTTGATCAGGATATTACTGTTTACAGACACTTCTTTATCTAATCTGGAAAAATATTTGGCAGCTTCGAGGATTTGAGCTATTAAAAGCAATTCATTTGGAACCATATCCCTTTGACATGGAGAGGGATCTGATAACTCCAACATTTAAATTGAAGAGACCACAGCTGCTTAAATACTACAAGGTATCACTTATCCCACTTTCGGTTAAATCCTAGCATTTCTACTGTTCTCTTTTGTTCTGAGCTTCTTTGCGTCAGTACCGAATCACGCATCAGAGCATGTTTTGAGTGTTAAACTGTGTATCATAACATCTGAGGCCGCTGCTTGATTCAGTGTAGTACTGTTTATGTTTAAATCGTTGCTGATTTTTTTTGTTGAATTCTGCTTTGGCCCTTCGGCTGTGTTTGAAAAAGTTACCCATATCATGAAAAAAACATTTATATGCATAGTTTCCCTTCTCTCTTTATATGCGCCCAAGGTAGCCAAGGGAAGTGCATATAACTTTACAATTGGCTGTGATTTGAGATGAATAGATATATATTTTTTTTAAAAAGAATTCTTCCTATGCACTCCCGGGCGAATCTATGGTAACTGCCTAAGCCCCCGTTAGCCGATATTGTTCTCTTTGGACTTTTCCTTTTGGACTTCCCCACAGTTTTTAAAATACAGTCTGCTAGGGAGCGGTTTCCACACCTTTATAAAGAATGTTTCGTTTTCCTCTTCAACCGATGTGGGATTTGGATTTAAAACTTTTTTTATAAGGGTGTGGAAACCTTTCCCTACCAAACGCGTTCTAAAAATCTTGAGGGGAAGTCCAAAAGGGAAATCCTAAAGAGGACGATATTTGCAAGCGGTGTTGCAAATTTCTTATGGAAAAGAGATTCTAGCCTTTGCAACTTAAAAAAAAAAAAACTAAACTACTCTCCTGCCTGTAATATTGGGCTATTGACATATAAAACACCACAAAATTCTTTGAAAAACAGTTTTAACAGAAATTGTTCCTGAAAAAGTGTTCCAGACAACAGTTGACCTCACCTTGAACTACGCTCAAAAGTCTATTTACAGCTTCTCGTACTTCAAACTTCGTTGTATGATGGGTGTTTATCTATTGATTTGCAATGCGAACTTTATTATGTTTCATTCTATTTGGTTACTCTCTTGTAATTCCACGTGCAGGAGAGAATTGATCAACTATATGAAGAGGCAAAGAAGGCAGCAAAACCGATAAGTTAAGTGTACAAATCTTAGTAGCGATCCTCGAAATCCTAATAAAAGGAGAGAAATATGTTAACTTATGAAGAAATTTTTGTGCTGACTTTGTGGATGAACTTGAAAATTCCATAGAAAATTAAGTCTTATTATAGAAGTTATCAAAACATGACTTCAATTCTAAACTTTGCACACAAAAACAAAGTAGAAAAATGTGCATTTGTTTACGGGAGATCCCACGTCGATGGGAGAGAAGAACAGAACATTTTTTATAAGGGTGTGGAAACCTTTACTTAACGGACGCGTTTTAAAAACTTTGAGGGGAAGCCCAAAGAGGACAATGTTCGCTAGCAGTGGGCTTGAACCATTACAAATATTGTTAGAGCTAGACATCGGATGATGTGCCAGTGGGAGGTTGAACCTTGAAAGGGATGGGGGGAGACACGAGGCAGTGTGCAAGCAAGGACATTGGGCCTTTAATGGAGGGTGGATTAGGGGGTCCCACATTGATTGAAGAACTGAAC

mRNA sequence

ACAAATTGGTGTAGTTTGCTGAGAATTTAATGGCATTCATCACACCCACCTCAACCATCCTCCCAACCGCCCACCTCTGGTTTAGATCAACTGTAATAAAGCCACTTCCACCGGACTTTTTCAGCAACCCACACCCACAAACAGACCAACCCAATCTTCCGCAGCCGTTTCCTTTCAAATTCCGGCCGTTTTCATGACAATGCCGACGGCCTACACGGTGGCCGTCGAGGATGCGACGCCGGAGAGCGGCGGAAAGCCGTCGGCGGGGCCGGTTTACCGGAGCATCTACGCTAAAGATGGGCTTCTCCAGTTGCTGGCTGGGTTCGAATCTCCATGGCAGTTCTTCAGCGATTCCGTCAAGAGGAATCCTGGAAACCGGATGCTTGGTCGTCGGCGAACCAACGGCGATTCCAAGGCGGGTTCCTATGTATGGCTCACATATCAAGAAGCGTACGATGCTGCAATCCGGATGGGTTCTGCTATTAGAAAGTGTGGCGTTAATCCTGGAGATTGCTGCGGTATATATGGATCCAATTGCCCTGAATGGATAATTTCGATGGAGGCCTGTAATAGCCAAGCTATCACTTACGTACCTCTGTATGATACACTAGGTGCTAATGCTGTCGAATTCATCATTAACCATGCTGAAGTTTCAATTGCTTTTGTTCAAGAAAACAAACTTGCTTCTATCTTTTCATGCCTTCCCAGGTGTTCGACTCGATTAAAAACTATTGTCAGTTTTACCAATGTTTCAAGCGTACATAAGAAGGAAGCCGAAGAACTGGGAGTATCTTGCTATTCTTGGGAGGAGTTTTATCAGTTGGGAAGCTTGGACTGTGAACTCCCTTCAAAACAGAAGACCAATATCTGCACAATAATGTATACAAGTGGAACCACCGGGGAACCGAAAGGTGTTATCTTAAATAATGCAGCTATAATGGCCGAAGTTTTGTCTGTGGACCACATACTTTTATTGACAGATAAAGTGGCGACAGAAGAAGATTCATATTTCTCATTTCTTCCTCTAGCCCATGTGTACGATCAGATAATGGAGACCTATTGCATCTACAAGGGTTCATCTATCGGGTTCTGGCGTGGGGACATCAGGTTTTTGCTGGATGATATTCAGGAACTGAAGCCAACAATATTTTCTGGAGTTCCTCGGGTCTATGATCGTATTTATTCTGGTATTCTGAACAAAGTTTCGTCCGGAGGTAGTTTGCGGAAGATGTTATTCGATTATGCATACAATTACAAGCTAGGATATCTGTCGAAGGGTGTGCCCCAGGAAAAAGCTGCACCTCTCTTGGACAAGCTTGTATTTGACAAGATAAAAGAAGCATTTGGTGGAAGAGTTCGTCTATTTTTATCTGGTGCTGCACCTTTGCCCAGGCATGTGGAAGAGTTCTTGAGGGTTACCAGTTGTGCAACTTTAAGCCAAGGATATGGCCTTACAGAAAGTTGTGGTGGATGTCTTACATCCATAGCCAATGTTTTCACCATGATGGGAACTGTTGGAGTTCCAGTTACTACCATTGAAGTAAGGCTCGAGTCAGTTCCTGATATGGGGTATGATGCACTGTCAAGCACACCTCGTGGAGAGATTTGCTTGAGGGGAAGTACTTTGTTCTCTGGATATCATAAACGGCAAGATCTAACAGAAGATGTTCTTATTGACGGATGGTTTCATACAGGGGACATTGGCGAATGGCAGCCTGATGGATCGATGAAAATAATTGATCGGAAAAAGAATATATTTAAGCTGTCCCAGGGGGAGTATGTTGCAGTGGAGAACATTGAAAACATATTCTTGCAATGCCCTCTGATTACCTCGATTTGGGTCTATGGAAATAGTTTCGAGTCATTTCTTGTGGGTGTGGCGGTTCCCGATCAGAAGGCCTTGGAGGATTGGGCACGGAACCATAATGTGACTGGTGACTTTAAATCTTTGTGTGAGAATCTCAGTGCAAGGAAGTATATTTTGGATGAGCTCAATAGCAATGGTCAGAAGCACCATCTTCGAGGATTTGAGCTATTAAAAGCAATTCATTTGGAACCATATCCCTTTGACATGGAGAGGGATCTGATAACTCCAACATTTAAATTGAAGAGACCACAGCTGCTTAAATACTACAAGGAGAGAATTGATCAACTATATGAAGAGGCAAAGAAGGCAGCAAAACCGATAAGTTAAGTGTACAAATCTTAGTAGCGATCCTCGAAATCCTAATAAAAGGAGAGAAATATGTTAACTTATGAAGAAATTTTTGTGCTGACTTTGTGGATGAACTTGAAAATTCCATAGAAAATTAAGTCTTATTATAGAAGTTATCAAAACATGACTTCAATTCTAAACTTTGCACACAAAAACAAAGTAGAAAAATGTGCATTTGTTTACGGGAGATCCCACGTCGATGGGAGAGAAGAACAGAACATTTTTTATAAGGGTGTGGAAACCTTTACTTAACGGACGCGTTTTAAAAACTTTGAGGGGAAGCCCAAAGAGGACAATGTTCGCTAGCAGTGGGCTTGAACCATTACAAATATTGTTAGAGCTAGACATCGGATGATGTGCCAGTGGGAGGTTGAACCTTGAAAGGGATGGGGGGAGACACGAGGCAGTGTGCAAGCAAGGACATTGGGCCTTTAATGGAGGGTGGATTAGGGGGTCCCACATTGATTGAAGAACTGAAC

Coding sequence (CDS)

ATGACAATGCCGACGGCCTACACGGTGGCCGTCGAGGATGCGACGCCGGAGAGCGGCGGAAAGCCGTCGGCGGGGCCGGTTTACCGGAGCATCTACGCTAAAGATGGGCTTCTCCAGTTGCTGGCTGGGTTCGAATCTCCATGGCAGTTCTTCAGCGATTCCGTCAAGAGGAATCCTGGAAACCGGATGCTTGGTCGTCGGCGAACCAACGGCGATTCCAAGGCGGGTTCCTATGTATGGCTCACATATCAAGAAGCGTACGATGCTGCAATCCGGATGGGTTCTGCTATTAGAAAGTGTGGCGTTAATCCTGGAGATTGCTGCGGTATATATGGATCCAATTGCCCTGAATGGATAATTTCGATGGAGGCCTGTAATAGCCAAGCTATCACTTACGTACCTCTGTATGATACACTAGGTGCTAATGCTGTCGAATTCATCATTAACCATGCTGAAGTTTCAATTGCTTTTGTTCAAGAAAACAAACTTGCTTCTATCTTTTCATGCCTTCCCAGGTGTTCGACTCGATTAAAAACTATTGTCAGTTTTACCAATGTTTCAAGCGTACATAAGAAGGAAGCCGAAGAACTGGGAGTATCTTGCTATTCTTGGGAGGAGTTTTATCAGTTGGGAAGCTTGGACTGTGAACTCCCTTCAAAACAGAAGACCAATATCTGCACAATAATGTATACAAGTGGAACCACCGGGGAACCGAAAGGTGTTATCTTAAATAATGCAGCTATAATGGCCGAAGTTTTGTCTGTGGACCACATACTTTTATTGACAGATAAAGTGGCGACAGAAGAAGATTCATATTTCTCATTTCTTCCTCTAGCCCATGTGTACGATCAGATAATGGAGACCTATTGCATCTACAAGGGTTCATCTATCGGGTTCTGGCGTGGGGACATCAGGTTTTTGCTGGATGATATTCAGGAACTGAAGCCAACAATATTTTCTGGAGTTCCTCGGGTCTATGATCGTATTTATTCTGGTATTCTGAACAAAGTTTCGTCCGGAGGTAGTTTGCGGAAGATGTTATTCGATTATGCATACAATTACAAGCTAGGATATCTGTCGAAGGGTGTGCCCCAGGAAAAAGCTGCACCTCTCTTGGACAAGCTTGTATTTGACAAGATAAAAGAAGCATTTGGTGGAAGAGTTCGTCTATTTTTATCTGGTGCTGCACCTTTGCCCAGGCATGTGGAAGAGTTCTTGAGGGTTACCAGTTGTGCAACTTTAAGCCAAGGATATGGCCTTACAGAAAGTTGTGGTGGATGTCTTACATCCATAGCCAATGTTTTCACCATGATGGGAACTGTTGGAGTTCCAGTTACTACCATTGAAGTAAGGCTCGAGTCAGTTCCTGATATGGGGTATGATGCACTGTCAAGCACACCTCGTGGAGAGATTTGCTTGAGGGGAAGTACTTTGTTCTCTGGATATCATAAACGGCAAGATCTAACAGAAGATGTTCTTATTGACGGATGGTTTCATACAGGGGACATTGGCGAATGGCAGCCTGATGGATCGATGAAAATAATTGATCGGAAAAAGAATATATTTAAGCTGTCCCAGGGGGAGTATGTTGCAGTGGAGAACATTGAAAACATATTCTTGCAATGCCCTCTGATTACCTCGATTTGGGTCTATGGAAATAGTTTCGAGTCATTTCTTGTGGGTGTGGCGGTTCCCGATCAGAAGGCCTTGGAGGATTGGGCACGGAACCATAATGTGACTGGTGACTTTAAATCTTTGTGTGAGAATCTCAGTGCAAGGAAGTATATTTTGGATGAGCTCAATAGCAATGGTCAGAAGCACCATCTTCGAGGATTTGAGCTATTAAAAGCAATTCATTTGGAACCATATCCCTTTGACATGGAGAGGGATCTGATAACTCCAACATTTAAATTGAAGAGACCACAGCTGCTTAAATACTACAAGGAGAGAATTGATCAACTATATGAAGAGGCAAAGAAGGCAGCAAAACCGATAAGTTAA

Protein sequence

MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPGNRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWIISMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTIVSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLSKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKKAAKPIS
Homology
BLAST of CmoCh20G008840 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 1054.7 bits (2726), Expect = 4.4e-307
Identity = 499/656 (76.07%), Postives = 572/656 (87.20%), Query Frame = 0

Query: 6   AYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPGNRMLG 65
           +YTV VE+A P +   PSAGPVYRSIYA+DGLL+L  G +SPW+F S SVKR+P   MLG
Sbjct: 2   SYTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPMLG 61

Query: 66  RRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWIISMEAC 125
           RR+   DS+AG YVWLTYQE +D AIRM SA+R  GVNPGD CGIYG+NCP+WI++M+AC
Sbjct: 62  RRQIK-DSEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQAC 121

Query: 126 NSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTIVSFTN 185
            S AITYVPLYDTLG NAVEFIINH EVSIAFVQENK+++I SCLP CS+ LKTIVSF N
Sbjct: 122 YSHAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFGN 181

Query: 186 VSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVILNN 245
           ++SV KKE E LGVSC+SWEEF QLG+L  ELP K +T+ICT+MYTSG TGEPKGVIL N
Sbjct: 182 ITSVQKKETEALGVSCFSWEEFSQLGNLSGELPEKHRTDICTLMYTSGATGEPKGVILTN 241

Query: 246 AAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRGDIR 305
            AIMAE+LS D++L LTDKV +EED YFS+LPLAHVYDQI+E YCIYKGS+IG+WRGDIR
Sbjct: 242 EAIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGDIR 301

Query: 306 FLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLSKGVPQ 365
           FL+DD+QELKPTIF GVPRVYDRIY+GI +KVSSGG+L+KMLF YAYNYK+  + KG+P 
Sbjct: 302 FLMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGLPH 361

Query: 366 EKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTESCG 425
            +AAPL+DKL FDKIK+ FGGR+RL  SGAAPLP HVEE+LRVTSCA LSQGYGLTESCG
Sbjct: 362 GQAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTESCG 421

Query: 426 GCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFSGYHKR 485
           GCLTSIAN+F M+GTVGVP+TTIE RLESVP+MGYDALS  PRGEICLRG+TLFSGYHKR
Sbjct: 422 GCLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYHKR 481

Query: 486 QDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQCPLI 545
           +DLT+DVL+DGWFHTGDIGEWQP+G+MKIIDRKKNIFKLSQGEYV VENIE I+LQCPLI
Sbjct: 482 EDLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCPLI 541

Query: 546 TSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDELNSNGQ 605
            SIWVYGNSFESFLV V VP++ ALE+WA N N+T DFKSLCEN  A KYILDELNS  +
Sbjct: 542 ASIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELNSTAK 601

Query: 606 KHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKKA 662
           KH LRGFE+LKA++LEP PFDMERDLITPTFKLKRPQLLKYYK+ +D+LY EAK+A
Sbjct: 602 KHQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYKDHVDKLYSEAKEA 656

BLAST of CmoCh20G008840 vs. ExPASy Swiss-Prot
Match: Q9XIA9 (Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 6.5e-274
Identity = 449/664 (67.62%), Postives = 544/664 (81.93%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           M++     + VE+  P +   PSAGPVYR  YAKDGLL L    +SPWQFFS++VK+ P 
Sbjct: 1   MSLAADNVLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPN 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
            +MLG+R T  DSK G Y W+TY+EA+DAAIR+GSAIR  GV+PG CCGIYG+NCPEWII
Sbjct: 61  EQMLGQRVTT-DSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           +MEAC SQ ITYVPLYD+LG NAVEFIINHAEVS+ FVQE  ++SI SC   CS+ LKTI
Sbjct: 121 AMEACMSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTI 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLD-CELPSKQKTNICTIMYTSGTTGEPK 240
           VSF  VSS  K+EA+   VS +SW EF  +G+LD   LP K+KT+ICTIMYTSGTTGEPK
Sbjct: 181 VSFGEVSSTQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPK 240

Query: 241 GVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGF 300
           GVILNNAAI  +VLS+D +L +TD+     D +FS+LPLAH YDQ+ME Y + +GSS+G+
Sbjct: 241 GVILNNAAISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGY 300

Query: 301 WRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYL 360
           WRGDIR+L+DD+Q LKPT+F GVPRVYD++Y+GI+ K+S+ G +RK LFD+AYNYKLG +
Sbjct: 301 WRGDIRYLMDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNM 360

Query: 361 SKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYG 420
            KG  QE+A+P LD+L+FDKIKEA GGR  + LSGAAPLPRHVEEFLR+   + LSQGYG
Sbjct: 361 RKGFSQEEASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYG 420

Query: 421 LTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSS-TPRGEICLRGSTL 480
           LTESCGG  T++A VF+M+GTVGVP+ T+E RL SVP+MGYDA S+  PRGEICLRG+++
Sbjct: 421 LTESCGGSFTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSM 480

Query: 481 FSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENI 540
           FSGYHKRQDLT+ VLIDGWFHTGDIGEWQ DGSMKIIDRKKNIFKLSQGEYVAVEN+EN 
Sbjct: 481 FSGYHKRQDLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENT 540

Query: 541 FLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWAR-NHNVTGDFKSLCENLSARKYIL 600
           + +CPLI  IWVYGNSFESFLVGV VPD+KA+EDWA+ N+    DF+SLC+NL A+KY L
Sbjct: 541 YSRCPLIAQIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFL 600

Query: 601 DELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEE 660
           DELNS  +++ L+GFE+LKAIHLEP PFD+ERDLITPTFKLKRPQLL++YK  +DQLY E
Sbjct: 601 DELNSTAKQYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSE 660

Query: 661 AKKA 662
           AK++
Sbjct: 661 AKRS 663

BLAST of CmoCh20G008840 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 3.7e-229
Identity = 373/657 (56.77%), Postives = 489/657 (74.43%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           M+    Y   VE+    S G+PS GPVYRSI+AKDG    + G +S W  F  SV++ P 
Sbjct: 1   MSQQKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPN 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           N MLGRR    D K G YVW TYQE YD  +++G+++R  GV     CGIYG+N PEWII
Sbjct: 61  NPMLGRREI-VDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACN+  +  VPLYDTLGA+AVEFII+H+EVSI FV+E K++ +F   P  +  +KT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTV 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLG-SLDCELPSKQKTNICTIMYTSGTTGEPK 240
           VSF  VS   K+EAE  G+  Y+W+EF +LG     +LP K+K++ICTIMYTSGTTG+PK
Sbjct: 181 VSFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPK 240

Query: 241 GVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGF 300
           GV+++N +I+  +  V  +L   ++  T +D Y S+LPLAH++D+++E   I  G++IGF
Sbjct: 241 GVMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGF 300

Query: 301 WRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYL 360
           WRGD++ L++D+ ELKPTIF  VPRV DR+YSG+  K+S GG L+K +FD A++YK GY+
Sbjct: 301 WRGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYM 360

Query: 361 SKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYG 420
            KG    +A+PL DKLVF K+K+  GG VR+ LSGAAPL  HVE FLRV +C  + QGYG
Sbjct: 361 KKGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYG 420

Query: 421 LTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLF 480
           LTESC G   S+ +   M+GTVG PV  +++RLESVP+M YDAL+ST RGEIC+RG TLF
Sbjct: 421 LTESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLF 480

Query: 481 SGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIF 540
           SGY+KR+DLT++VLIDGW HTGD+GEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENI+
Sbjct: 481 SGYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIY 540

Query: 541 LQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDE 600
            +   + S+WVYGNSFESFL+ +A P+Q  LE WA  + V+GD+ +LC+N  A+++IL E
Sbjct: 541 GEVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGE 600

Query: 601 LNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYE 657
           L    ++  ++GFE++KAIHL+P PFDMERDL+TPTFK KRPQLLKYY+  ID++Y+
Sbjct: 601 LVKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYK 656

BLAST of CmoCh20G008840 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 5.4e-228
Identity = 370/656 (56.40%), Postives = 488/656 (74.39%), Query Frame = 0

Query: 7   YTVAVEDATP-ESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPGNRMLG 66
           Y V VE       GG PS GPVYRSIYAKDG  +      S W  F  SV+++P N MLG
Sbjct: 6   YIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPMLG 65

Query: 67  RRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWIISMEAC 126
           RR    D KAG YVW TY+E ++  I++G++IR  GV  GD CGIYG+N PEWIISMEAC
Sbjct: 66  RREI-VDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEAC 125

Query: 127 NSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTIVSFTN 186
           N+  +  VPLYDTLGA A+EFII HAEVS+AF +ENK++ +    P+ +  LK IVSF  
Sbjct: 126 NAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGE 185

Query: 187 VSSVHKKEAEELGVSCYSWEEFYQLG-SLDCELPSKQKTNICTIMYTSGTTGEPKGVILN 246
           V++  + EAE   ++ YSW++F +LG     ELP K+++++CTIMYTSGTTG+PKGV+L 
Sbjct: 186 VTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLT 245

Query: 247 NAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRGDI 306
           N +I+  +  V  +L   D+  T +D Y S+LPLAH++D+++E  CIY+ +SIGFWRGD+
Sbjct: 246 NESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDV 305

Query: 307 RFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLSKGVP 366
           + L++DI  LKPT+F  VPRV +RIY+G+  K+S GG ++K LF++A+ YK   + KG P
Sbjct: 306 KILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQP 365

Query: 367 QEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTESC 426
            E+A+P+ DK+VF K+KE  GG VRL LSGAAPL  H+E FLRV +CA + QGYGLTESC
Sbjct: 366 HEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESC 425

Query: 427 GGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFSGYHK 486
           GG   SI N  +M+GTVG PV  +++RLESVP+MGYDAL+S PRGEIC+RG TLFSGY+K
Sbjct: 426 GGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYK 485

Query: 487 RQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQCPL 546
           R+DLT++V IDGW HTGD+GEWQPDG+MKIIDRKKNIFKLSQGEYVAVEN+ENI+     
Sbjct: 486 REDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAA 545

Query: 547 ITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDELNSNG 606
           I SIWVYGNS+ES+LV V  P +  +E WA+ H V+GDF+S+C N   ++++L E N   
Sbjct: 546 IESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVA 605

Query: 607 QKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 661
           +   L+GFEL+K +HL+  PFDMERDLITP++K+KRPQLLKYY++ ID++Y++ ++
Sbjct: 606 KDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYKKNRE 660

BLAST of CmoCh20G008840 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 8.6e-226
Identity = 368/662 (55.59%), Postives = 483/662 (72.96%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           MT    +   VE A   + G PS GPVYRS +A++G    + G +S W  F  +V++ P 
Sbjct: 1   MTSQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPN 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           NRMLGRR  + + KAG YVW TY+E YD  I++G+++R CG+  G+ CGIYG NC EWII
Sbjct: 61  NRMLGRREIS-NGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACN+  +  VPLYDTLGA AVEFII+HAEVSIAFV+E K+  +F   P  +  +KT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTV 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLG-SLDCELPSKQKTNICTIMYTSGTTGEPK 240
           VSF  V    K+EAE+LG+  +SW+EF +LG     ELP K+ ++ICTIMYTSGTTG+PK
Sbjct: 181 VSFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPK 240

Query: 241 GVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGF 300
           GV+++N +I+     V H L   +   +E+D Y S+LPLAHV+D+ +E   I  G SIGF
Sbjct: 241 GVMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGF 300

Query: 301 WRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYL 360
           WRGD++ L++D+ ELKP+IF  VPRV DR+Y+G+  K+S GG  +K +FD A++YK G +
Sbjct: 301 WRGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNM 360

Query: 361 SKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYG 420
            KG     A+P  DKLVF+K+K+  GG VR+ LSGAAPL  H+E FLRV +C  + QGYG
Sbjct: 361 KKGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYG 420

Query: 421 LTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLF 480
           LTESC G   +  +   M+GTVG PV  +++RLESVP+M YDAL STPRGEIC+RG TLF
Sbjct: 421 LTESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLF 480

Query: 481 SGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIF 540
           SGY+KR+DLT++V IDGW HTGD+GEWQP+GSMKIIDRKKNIFKL+QGEYVAVEN+EN++
Sbjct: 481 SGYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVY 540

Query: 541 LQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDE 600
            Q  +I SIWVYGNSFESFLV +A P Q+ LE WA  + V GDF S+C+N  A+ +IL E
Sbjct: 541 SQVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGE 600

Query: 601 LNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAK 660
           L    +++ L+GFE++K +HLEP  FDMERDL+TPT+K KRPQLLKYY+  I ++Y+  K
Sbjct: 601 LVKTAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTK 660

Query: 661 KA 662
           ++
Sbjct: 661 ES 661

BLAST of CmoCh20G008840 vs. ExPASy TrEMBL
Match: A0A6J1FW80 (long chain acyl-CoA synthetase 2 OS=Cucurbita moschata OX=3662 GN=LOC111449056 PE=4 SV=1)

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 666/666 (100.00%), Postives = 666/666 (100.00%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG
Sbjct: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII
Sbjct: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI
Sbjct: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240
           VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG
Sbjct: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240

Query: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300
           VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW
Sbjct: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300

Query: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360
           RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS
Sbjct: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360

Query: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420
           KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL
Sbjct: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420

Query: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480
           TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS
Sbjct: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480

Query: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540
           GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL
Sbjct: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540

Query: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL 600
           QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL
Sbjct: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL 600

Query: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 660
           NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK
Sbjct: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 660

Query: 661 AAKPIS 667
           AAKPIS
Sbjct: 661 AAKPIS 666

BLAST of CmoCh20G008840 vs. ExPASy TrEMBL
Match: A0A6J1JE62 (long chain acyl-CoA synthetase 2 OS=Cucurbita maxima OX=3661 GN=LOC111484205 PE=4 SV=1)

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 656/666 (98.50%), Postives = 663/666 (99.55%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           MTMPTAYTVAVEDATPESGG+PSAGPVYRSIYAKDGLLQL  GFESPWQFFSDSVKRNPG
Sbjct: 1   MTMPTAYTVAVEDATPESGGQPSAGPVYRSIYAKDGLLQLPTGFESPWQFFSDSVKRNPG 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           NRMLGRR+TNGDSKAGSYVWLTYQEAYD+AIRMGSAIRKCGVNPGDCCGIYGSNCPEWII
Sbjct: 61  NRMLGRRQTNGDSKAGSYVWLTYQEAYDSAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCST+LKTI
Sbjct: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTQLKTI 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240
           VSF NVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG
Sbjct: 181 VSFNNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240

Query: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300
           VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW
Sbjct: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300

Query: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360
           RGDIRFLL+DIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS
Sbjct: 301 RGDIRFLLNDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360

Query: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420
           KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL
Sbjct: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420

Query: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480
           TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS
Sbjct: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480

Query: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540
           GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL
Sbjct: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540

Query: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL 600
           QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENL+ARKYILDEL
Sbjct: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLNARKYILDEL 600

Query: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 660
           NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRP+LLKYYKERIDQLYEEAKK
Sbjct: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPRLLKYYKERIDQLYEEAKK 660

Query: 661 AAKPIS 667
           AAKPIS
Sbjct: 661 AAKPIS 666

BLAST of CmoCh20G008840 vs. ExPASy TrEMBL
Match: A0A1S3CAY4 (long chain acyl-CoA synthetase 2 OS=Cucumis melo OX=3656 GN=LOC103498426 PE=4 SV=1)

HSP 1 Score: 1275.0 bits (3298), Expect = 0.0e+00
Identity = 623/661 (94.25%), Postives = 638/661 (96.52%), Query Frame = 0

Query: 3   MPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPGNR 62
           M T YTV VE  TPE+GG+PSAGPVYRSIYAKDGLLQL    ESPWQFFSDSVKRNPGNR
Sbjct: 1   MSTPYTVVVEAETPETGGRPSAGPVYRSIYAKDGLLQLPDELESPWQFFSDSVKRNPGNR 60

Query: 63  MLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWIISM 122
           MLGRR+TNGDSKAGSYVWLTYQEAYDAAIRMGSAIRK GVN GD CGIYGSNCPEWIISM
Sbjct: 61  MLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNCPEWIISM 120

Query: 123 EACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTIVS 182
           EACNSQAITYVPLYDTLGANAVEFI+NHAEVSIAFVQENKLASIFSCLPRCS +LKTIVS
Sbjct: 121 EACNSQAITYVPLYDTLGANAVEFIVNHAEVSIAFVQENKLASIFSCLPRCSNQLKTIVS 180

Query: 183 FTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI 242
           FTNVSSVHKKEAEELGVSC+SWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI
Sbjct: 181 FTNVSSVHKKEAEELGVSCFSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI 240

Query: 243 LNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG 302
           LNNAAIMAEVLSVDH+LLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG
Sbjct: 241 LNNAAIMAEVLSVDHLLLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG 300

Query: 303 DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLSKG 362
           DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAY YKLGYL KG
Sbjct: 301 DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYTYKLGYLKKG 360

Query: 363 VPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTE 422
           + QEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPR VEEFLRVTSCATLSQGYGLTE
Sbjct: 361 LLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRLVEEFLRVTSCATLSQGYGLTE 420

Query: 423 SCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFSGY 482
           SCGGC TSIANVFTM+GTVGVPVTTIE RLESVPDMGYDAL++TPRGEIC+RGSTLFSGY
Sbjct: 421 SCGGCFTSIANVFTMVGTVGVPVTTIEARLESVPDMGYDALATTPRGEICIRGSTLFSGY 480

Query: 483 HKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC 542
           HKRQDLT+DVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC
Sbjct: 481 HKRQDLTDDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC 540

Query: 543 PLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDELNS 602
           PLITSIWVYGNSFESFLVGVAVPDQKALEDWA+NHNVTGDFKSLCENL ARKYILDELNS
Sbjct: 541 PLITSIWVYGNSFESFLVGVAVPDQKALEDWAQNHNVTGDFKSLCENLKARKYILDELNS 600

Query: 603 NGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKKAA 662
           NGQKH LRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKE+IDQLYEEAK A 
Sbjct: 601 NGQKHRLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKEQIDQLYEEAKNAK 660

Query: 663 K 664
           K
Sbjct: 661 K 661

BLAST of CmoCh20G008840 vs. ExPASy TrEMBL
Match: A0A0A0LL21 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G238260 PE=4 SV=1)

HSP 1 Score: 1258.8 bits (3256), Expect = 0.0e+00
Identity = 616/661 (93.19%), Postives = 635/661 (96.07%), Query Frame = 0

Query: 3   MPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPGNR 62
           M T+YTV VE  TP+SGG+PSAGPVYRSIYAKDGLLQL    +SPWQFFSDSVKRNPGN 
Sbjct: 1   MSTSYTVVVEADTPQSGGRPSAGPVYRSIYAKDGLLQLPDELQSPWQFFSDSVKRNPGNP 60

Query: 63  MLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWIISM 122
           MLGRR+TNGDSKAGSYVWLTYQEAYDAAIRMGSAIRK GVN GD CGIYGSNCPEWIISM
Sbjct: 61  MLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNCPEWIISM 120

Query: 123 EACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTIVS 182
           EACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCS +LKTIVS
Sbjct: 121 EACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSNQLKTIVS 180

Query: 183 FTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI 242
           FTNVSSV KKEAEELGVSC+SWEEF QLGSLDCELPSKQKT+ICTIMYTSGTTGEPKGVI
Sbjct: 181 FTNVSSVLKKEAEELGVSCFSWEEFCQLGSLDCELPSKQKTDICTIMYTSGTTGEPKGVI 240

Query: 243 LNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG 302
           LNN A+MAEVLSV+H+LLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG
Sbjct: 241 LNNTAVMAEVLSVEHLLLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG 300

Query: 303 DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLSKG 362
           DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAY YKLGYL KG
Sbjct: 301 DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYIYKLGYLKKG 360

Query: 363 VPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTE 422
           + QEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTE
Sbjct: 361 LLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTE 420

Query: 423 SCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFSGY 482
           SCGGC TSIANVFTMMGTVGVPVTTIE RLESVPDMGYDAL++TPRGEICLRGSTLFSGY
Sbjct: 421 SCGGCFTSIANVFTMMGTVGVPVTTIEARLESVPDMGYDALATTPRGEICLRGSTLFSGY 480

Query: 483 HKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC 542
           HKRQDLT +VLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC
Sbjct: 481 HKRQDLTGEVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC 540

Query: 543 PLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDELNS 602
            LITSIWVYGNSFESFLVGVAVPDQKALEDWA+NHN+TGDFKSLCENL ARKYILDELNS
Sbjct: 541 SLITSIWVYGNSFESFLVGVAVPDQKALEDWAQNHNMTGDFKSLCENLKARKYILDELNS 600

Query: 603 NGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKKAA 662
           NGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKE+ID+LYEEAK A 
Sbjct: 601 NGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKEQIDELYEEAKNAK 660

Query: 663 K 664
           K
Sbjct: 661 K 661

BLAST of CmoCh20G008840 vs. ExPASy TrEMBL
Match: A0A6J1D4L4 (long chain acyl-CoA synthetase 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017256 PE=4 SV=1)

HSP 1 Score: 1256.9 bits (3251), Expect = 0.0e+00
Identity = 608/657 (92.54%), Postives = 634/657 (96.50%), Query Frame = 0

Query: 3   MPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPGNR 62
           MP AYTVAVE+ATPES G+PSAGPVYRSIYAKDGLLQL AG +SPWQFFSDSVKRNPGNR
Sbjct: 1   MPAAYTVAVEEATPESSGRPSAGPVYRSIYAKDGLLQLPAGLDSPWQFFSDSVKRNPGNR 60

Query: 63  MLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWIISM 122
           MLGRR+TNGDSKAGSYVWLTYQEAYDAAIRMGSAIR  GVNPGD CGIYGSNCPEWII+M
Sbjct: 61  MLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRNRGVNPGDRCGIYGSNCPEWIIAM 120

Query: 123 EACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTIVS 182
           EACNSQA+TYVPLYDTLGANAVEFIINHAEVSIAFVQE+KLASIFSCLPRCST+LKTIVS
Sbjct: 121 EACNSQAVTYVPLYDTLGANAVEFIINHAEVSIAFVQESKLASIFSCLPRCSTQLKTIVS 180

Query: 183 FTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI 242
           FTNVSS  K+EAEELGVSC+SW+EFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI
Sbjct: 181 FTNVSSACKEEAEELGVSCFSWDEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI 240

Query: 243 LNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG 302
           LNN+AIM EVLSVDH+LLLTDKV TEEDSYFSFLPLAHVYDQIMETYCI KGSSIGFWRG
Sbjct: 241 LNNSAIMTEVLSVDHLLLLTDKVGTEEDSYFSFLPLAHVYDQIMETYCICKGSSIGFWRG 300

Query: 303 DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLSKG 362
           DIRFLLDDIQELKPTIFSGVPRVYDRIY+G+LNKVSSGG+LRKMLFDYAYNYKLGYL KG
Sbjct: 301 DIRFLLDDIQELKPTIFSGVPRVYDRIYTGVLNKVSSGGALRKMLFDYAYNYKLGYLEKG 360

Query: 363 VPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTE 422
           + Q+KAAPLLDKLVF+KIKEAFGGRVRL LSGAAPLPRHVEEFLRVTSCATLSQGYGLTE
Sbjct: 361 LLQDKAAPLLDKLVFEKIKEAFGGRVRLLLSGAAPLPRHVEEFLRVTSCATLSQGYGLTE 420

Query: 423 SCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFSGY 482
           SCGGC TSIANVF+MMGTVGVPVTTIE RLESVPDMGYDALSSTPRGEICLRGSTLFSGY
Sbjct: 421 SCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDALSSTPRGEICLRGSTLFSGY 480

Query: 483 HKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC 542
           HKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENI+ QC
Sbjct: 481 HKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYSQC 540

Query: 543 PLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDELNS 602
           PL+TSIWVYGNSFESFLVGV VPDQKALEDWA NHNVTGDFKSLCENL ARKYILDELNS
Sbjct: 541 PLVTSIWVYGNSFESFLVGVVVPDQKALEDWAMNHNVTGDFKSLCENLKARKYILDELNS 600

Query: 603 NGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAK 660
           NGQK  +RGFELLKAIHLEPYPFDMERDLITPTFKLKRP+LLKYYKE+IDQLY+EAK
Sbjct: 601 NGQKGQIRGFELLKAIHLEPYPFDMERDLITPTFKLKRPELLKYYKEQIDQLYKEAK 657

BLAST of CmoCh20G008840 vs. NCBI nr
Match: XP_022944667.1 (long chain acyl-CoA synthetase 2 [Cucurbita moschata])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 666/666 (100.00%), Postives = 666/666 (100.00%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG
Sbjct: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII
Sbjct: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI
Sbjct: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240
           VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG
Sbjct: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240

Query: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300
           VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW
Sbjct: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300

Query: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360
           RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS
Sbjct: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360

Query: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420
           KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL
Sbjct: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420

Query: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480
           TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS
Sbjct: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480

Query: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540
           GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL
Sbjct: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540

Query: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL 600
           QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL
Sbjct: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL 600

Query: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 660
           NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK
Sbjct: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 660

Query: 661 AAKPIS 667
           AAKPIS
Sbjct: 661 AAKPIS 666

BLAST of CmoCh20G008840 vs. NCBI nr
Match: XP_023512617.1 (long chain acyl-CoA synthetase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1348.2 bits (3488), Expect = 0.0e+00
Identity = 658/666 (98.80%), Postives = 661/666 (99.25%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           MTMPTAYTVAVEDATPESGGKPS GPVYRSIYAKDGLLQL  GFESPWQFFSDSVKRNPG
Sbjct: 1   MTMPTAYTVAVEDATPESGGKPSGGPVYRSIYAKDGLLQLPTGFESPWQFFSDSVKRNPG 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           NRMLGRR+TNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII
Sbjct: 61  NRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCST+LKTI
Sbjct: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTQLKTI 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240
           VSF NVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSK KTNICTIMYTSGTTGEPKG
Sbjct: 181 VSFNNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKLKTNICTIMYTSGTTGEPKG 240

Query: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300
           VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW
Sbjct: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300

Query: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360
           RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS
Sbjct: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360

Query: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420
           KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL
Sbjct: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420

Query: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480
           TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS
Sbjct: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480

Query: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540
           GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL
Sbjct: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540

Query: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL 600
           QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENL+ARKYILDEL
Sbjct: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLNARKYILDEL 600

Query: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 660
           NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK
Sbjct: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 660

Query: 661 AAKPIS 667
           AAKPIS
Sbjct: 661 AAKPIS 666

BLAST of CmoCh20G008840 vs. NCBI nr
Match: XP_022986475.1 (long chain acyl-CoA synthetase 2 [Cucurbita maxima])

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 656/666 (98.50%), Postives = 663/666 (99.55%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           MTMPTAYTVAVEDATPESGG+PSAGPVYRSIYAKDGLLQL  GFESPWQFFSDSVKRNPG
Sbjct: 1   MTMPTAYTVAVEDATPESGGQPSAGPVYRSIYAKDGLLQLPTGFESPWQFFSDSVKRNPG 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           NRMLGRR+TNGDSKAGSYVWLTYQEAYD+AIRMGSAIRKCGVNPGDCCGIYGSNCPEWII
Sbjct: 61  NRMLGRRQTNGDSKAGSYVWLTYQEAYDSAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCST+LKTI
Sbjct: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTQLKTI 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240
           VSF NVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG
Sbjct: 181 VSFNNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240

Query: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300
           VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW
Sbjct: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300

Query: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360
           RGDIRFLL+DIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS
Sbjct: 301 RGDIRFLLNDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360

Query: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420
           KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL
Sbjct: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420

Query: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480
           TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS
Sbjct: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480

Query: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540
           GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL
Sbjct: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540

Query: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL 600
           QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENL+ARKYILDEL
Sbjct: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLNARKYILDEL 600

Query: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 660
           NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRP+LLKYYKERIDQLYEEAKK
Sbjct: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPRLLKYYKERIDQLYEEAKK 660

Query: 661 AAKPIS 667
           AAKPIS
Sbjct: 661 AAKPIS 666

BLAST of CmoCh20G008840 vs. NCBI nr
Match: KAG6571141.1 (Long chain acyl-CoA synthetase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 657/666 (98.65%), Postives = 657/666 (98.65%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           MTMPT YTVAVEDATPESGG PSAGPVYRSIYAKDGLLQL  GFESPWQFFSDSVKRNPG
Sbjct: 1   MTMPTPYTVAVEDATPESGGNPSAGPVYRSIYAKDGLLQLPTGFESPWQFFSDSVKRNPG 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII
Sbjct: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI
Sbjct: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240
           V FTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG
Sbjct: 181 VCFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKG 240

Query: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300
           VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW
Sbjct: 241 VILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFW 300

Query: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360
           RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS
Sbjct: 301 RGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLS 360

Query: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGL 420
           KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGY  
Sbjct: 361 KGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGY-- 420

Query: 421 TESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480
            ESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS
Sbjct: 421 -ESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFS 480

Query: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540
           GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL
Sbjct: 481 GYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFL 540

Query: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL 600
           QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL
Sbjct: 541 QCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDEL 600

Query: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 660
           NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERI QLYEEAKK
Sbjct: 601 NSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIAQLYEEAKK 660

Query: 661 AAKPIS 667
           AAKPIS
Sbjct: 661 AAKPIS 663

BLAST of CmoCh20G008840 vs. NCBI nr
Match: XP_008459243.1 (PREDICTED: long chain acyl-CoA synthetase 2 [Cucumis melo])

HSP 1 Score: 1275.0 bits (3298), Expect = 0.0e+00
Identity = 623/661 (94.25%), Postives = 638/661 (96.52%), Query Frame = 0

Query: 3   MPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPGNR 62
           M T YTV VE  TPE+GG+PSAGPVYRSIYAKDGLLQL    ESPWQFFSDSVKRNPGNR
Sbjct: 1   MSTPYTVVVEAETPETGGRPSAGPVYRSIYAKDGLLQLPDELESPWQFFSDSVKRNPGNR 60

Query: 63  MLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWIISM 122
           MLGRR+TNGDSKAGSYVWLTYQEAYDAAIRMGSAIRK GVN GD CGIYGSNCPEWIISM
Sbjct: 61  MLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNCPEWIISM 120

Query: 123 EACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTIVS 182
           EACNSQAITYVPLYDTLGANAVEFI+NHAEVSIAFVQENKLASIFSCLPRCS +LKTIVS
Sbjct: 121 EACNSQAITYVPLYDTLGANAVEFIVNHAEVSIAFVQENKLASIFSCLPRCSNQLKTIVS 180

Query: 183 FTNVSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI 242
           FTNVSSVHKKEAEELGVSC+SWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI
Sbjct: 181 FTNVSSVHKKEAEELGVSCFSWEEFYQLGSLDCELPSKQKTNICTIMYTSGTTGEPKGVI 240

Query: 243 LNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG 302
           LNNAAIMAEVLSVDH+LLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG
Sbjct: 241 LNNAAIMAEVLSVDHLLLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRG 300

Query: 303 DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLSKG 362
           DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAY YKLGYL KG
Sbjct: 301 DIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYTYKLGYLKKG 360

Query: 363 VPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTE 422
           + QEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPR VEEFLRVTSCATLSQGYGLTE
Sbjct: 361 LLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRLVEEFLRVTSCATLSQGYGLTE 420

Query: 423 SCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFSGY 482
           SCGGC TSIANVFTM+GTVGVPVTTIE RLESVPDMGYDAL++TPRGEIC+RGSTLFSGY
Sbjct: 421 SCGGCFTSIANVFTMVGTVGVPVTTIEARLESVPDMGYDALATTPRGEICIRGSTLFSGY 480

Query: 483 HKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC 542
           HKRQDLT+DVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC
Sbjct: 481 HKRQDLTDDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQC 540

Query: 543 PLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDELNS 602
           PLITSIWVYGNSFESFLVGVAVPDQKALEDWA+NHNVTGDFKSLCENL ARKYILDELNS
Sbjct: 541 PLITSIWVYGNSFESFLVGVAVPDQKALEDWAQNHNVTGDFKSLCENLKARKYILDELNS 600

Query: 603 NGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKKAA 662
           NGQKH LRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKE+IDQLYEEAK A 
Sbjct: 601 NGQKHRLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKEQIDQLYEEAKNAK 660

Query: 663 K 664
           K
Sbjct: 661 K 661

BLAST of CmoCh20G008840 vs. TAIR 10
Match: AT1G49430.1 (long-chain acyl-CoA synthetase 2 )

HSP 1 Score: 944.5 bits (2440), Expect = 4.6e-275
Identity = 449/664 (67.62%), Postives = 544/664 (81.93%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           M++     + VE+  P +   PSAGPVYR  YAKDGLL L    +SPWQFFS++VK+ P 
Sbjct: 1   MSLAADNVLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPN 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
            +MLG+R T  DSK G Y W+TY+EA+DAAIR+GSAIR  GV+PG CCGIYG+NCPEWII
Sbjct: 61  EQMLGQRVTT-DSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           +MEAC SQ ITYVPLYD+LG NAVEFIINHAEVS+ FVQE  ++SI SC   CS+ LKTI
Sbjct: 121 AMEACMSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTI 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLGSLD-CELPSKQKTNICTIMYTSGTTGEPK 240
           VSF  VSS  K+EA+   VS +SW EF  +G+LD   LP K+KT+ICTIMYTSGTTGEPK
Sbjct: 181 VSFGEVSSTQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPK 240

Query: 241 GVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGF 300
           GVILNNAAI  +VLS+D +L +TD+     D +FS+LPLAH YDQ+ME Y + +GSS+G+
Sbjct: 241 GVILNNAAISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGY 300

Query: 301 WRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYL 360
           WRGDIR+L+DD+Q LKPT+F GVPRVYD++Y+GI+ K+S+ G +RK LFD+AYNYKLG +
Sbjct: 301 WRGDIRYLMDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNM 360

Query: 361 SKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYG 420
            KG  QE+A+P LD+L+FDKIKEA GGR  + LSGAAPLPRHVEEFLR+   + LSQGYG
Sbjct: 361 RKGFSQEEASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYG 420

Query: 421 LTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSS-TPRGEICLRGSTL 480
           LTESCGG  T++A VF+M+GTVGVP+ T+E RL SVP+MGYDA S+  PRGEICLRG+++
Sbjct: 421 LTESCGGSFTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSM 480

Query: 481 FSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENI 540
           FSGYHKRQDLT+ VLIDGWFHTGDIGEWQ DGSMKIIDRKKNIFKLSQGEYVAVEN+EN 
Sbjct: 481 FSGYHKRQDLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENT 540

Query: 541 FLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWAR-NHNVTGDFKSLCENLSARKYIL 600
           + +CPLI  IWVYGNSFESFLVGV VPD+KA+EDWA+ N+    DF+SLC+NL A+KY L
Sbjct: 541 YSRCPLIAQIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFL 600

Query: 601 DELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEE 660
           DELNS  +++ L+GFE+LKAIHLEP PFD+ERDLITPTFKLKRPQLL++YK  +DQLY E
Sbjct: 601 DELNSTAKQYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSE 660

Query: 661 AKKA 662
           AK++
Sbjct: 661 AKRS 663

BLAST of CmoCh20G008840 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 795.8 bits (2054), Expect = 2.6e-230
Identity = 373/657 (56.77%), Postives = 489/657 (74.43%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           M+    Y   VE+    S G+PS GPVYRSI+AKDG    + G +S W  F  SV++ P 
Sbjct: 1   MSQQKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPN 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           N MLGRR    D K G YVW TYQE YD  +++G+++R  GV     CGIYG+N PEWII
Sbjct: 61  NPMLGRREI-VDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACN+  +  VPLYDTLGA+AVEFII+H+EVSI FV+E K++ +F   P  +  +KT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTV 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLG-SLDCELPSKQKTNICTIMYTSGTTGEPK 240
           VSF  VS   K+EAE  G+  Y+W+EF +LG     +LP K+K++ICTIMYTSGTTG+PK
Sbjct: 181 VSFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPK 240

Query: 241 GVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGF 300
           GV+++N +I+  +  V  +L   ++  T +D Y S+LPLAH++D+++E   I  G++IGF
Sbjct: 241 GVMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGF 300

Query: 301 WRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYL 360
           WRGD++ L++D+ ELKPTIF  VPRV DR+YSG+  K+S GG L+K +FD A++YK GY+
Sbjct: 301 WRGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYM 360

Query: 361 SKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYG 420
            KG    +A+PL DKLVF K+K+  GG VR+ LSGAAPL  HVE FLRV +C  + QGYG
Sbjct: 361 KKGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYG 420

Query: 421 LTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLF 480
           LTESC G   S+ +   M+GTVG PV  +++RLESVP+M YDAL+ST RGEIC+RG TLF
Sbjct: 421 LTESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLF 480

Query: 481 SGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIF 540
           SGY+KR+DLT++VLIDGW HTGD+GEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENI+
Sbjct: 481 SGYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIY 540

Query: 541 LQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDE 600
            +   + S+WVYGNSFESFL+ +A P+Q  LE WA  + V+GD+ +LC+N  A+++IL E
Sbjct: 541 GEVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGE 600

Query: 601 LNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYE 657
           L    ++  ++GFE++KAIHL+P PFDMERDL+TPTFK KRPQLLKYY+  ID++Y+
Sbjct: 601 LVKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYK 656

BLAST of CmoCh20G008840 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 792.0 bits (2044), Expect = 3.8e-229
Identity = 370/656 (56.40%), Postives = 488/656 (74.39%), Query Frame = 0

Query: 7   YTVAVEDATP-ESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPGNRMLG 66
           Y V VE       GG PS GPVYRSIYAKDG  +      S W  F  SV+++P N MLG
Sbjct: 6   YIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPMLG 65

Query: 67  RRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWIISMEAC 126
           RR    D KAG YVW TY+E ++  I++G++IR  GV  GD CGIYG+N PEWIISMEAC
Sbjct: 66  RREI-VDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEAC 125

Query: 127 NSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTIVSFTN 186
           N+  +  VPLYDTLGA A+EFII HAEVS+AF +ENK++ +    P+ +  LK IVSF  
Sbjct: 126 NAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGE 185

Query: 187 VSSVHKKEAEELGVSCYSWEEFYQLG-SLDCELPSKQKTNICTIMYTSGTTGEPKGVILN 246
           V++  + EAE   ++ YSW++F +LG     ELP K+++++CTIMYTSGTTG+PKGV+L 
Sbjct: 186 VTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLT 245

Query: 247 NAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRGDI 306
           N +I+  +  V  +L   D+  T +D Y S+LPLAH++D+++E  CIY+ +SIGFWRGD+
Sbjct: 246 NESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDV 305

Query: 307 RFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLSKGVP 366
           + L++DI  LKPT+F  VPRV +RIY+G+  K+S GG ++K LF++A+ YK   + KG P
Sbjct: 306 KILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQP 365

Query: 367 QEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTESC 426
            E+A+P+ DK+VF K+KE  GG VRL LSGAAPL  H+E FLRV +CA + QGYGLTESC
Sbjct: 366 HEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESC 425

Query: 427 GGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFSGYHK 486
           GG   SI N  +M+GTVG PV  +++RLESVP+MGYDAL+S PRGEIC+RG TLFSGY+K
Sbjct: 426 GGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYK 485

Query: 487 RQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQCPL 546
           R+DLT++V IDGW HTGD+GEWQPDG+MKIIDRKKNIFKLSQGEYVAVEN+ENI+     
Sbjct: 486 REDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAA 545

Query: 547 ITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDELNSNG 606
           I SIWVYGNS+ES+LV V  P +  +E WA+ H V+GDF+S+C N   ++++L E N   
Sbjct: 546 IESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVA 605

Query: 607 QKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKK 661
           +   L+GFEL+K +HL+  PFDMERDLITP++K+KRPQLLKYY++ ID++Y++ ++
Sbjct: 606 KDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYKKNRE 660

BLAST of CmoCh20G008840 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 784.6 bits (2025), Expect = 6.1e-227
Identity = 368/662 (55.59%), Postives = 483/662 (72.96%), Query Frame = 0

Query: 1   MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPG 60
           MT    +   VE A   + G PS GPVYRS +A++G    + G +S W  F  +V++ P 
Sbjct: 1   MTSQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPN 60

Query: 61  NRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWII 120
           NRMLGRR  + + KAG YVW TY+E YD  I++G+++R CG+  G+ CGIYG NC EWII
Sbjct: 61  NRMLGRREIS-NGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWII 120

Query: 121 SMEACNSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTI 180
           SMEACN+  +  VPLYDTLGA AVEFII+HAEVSIAFV+E K+  +F   P  +  +KT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTV 180

Query: 181 VSFTNVSSVHKKEAEELGVSCYSWEEFYQLG-SLDCELPSKQKTNICTIMYTSGTTGEPK 240
           VSF  V    K+EAE+LG+  +SW+EF +LG     ELP K+ ++ICTIMYTSGTTG+PK
Sbjct: 181 VSFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPK 240

Query: 241 GVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGF 300
           GV+++N +I+     V H L   +   +E+D Y S+LPLAHV+D+ +E   I  G SIGF
Sbjct: 241 GVMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGF 300

Query: 301 WRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYL 360
           WRGD++ L++D+ ELKP+IF  VPRV DR+Y+G+  K+S GG  +K +FD A++YK G +
Sbjct: 301 WRGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNM 360

Query: 361 SKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYG 420
            KG     A+P  DKLVF+K+K+  GG VR+ LSGAAPL  H+E FLRV +C  + QGYG
Sbjct: 361 KKGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYG 420

Query: 421 LTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLF 480
           LTESC G   +  +   M+GTVG PV  +++RLESVP+M YDAL STPRGEIC+RG TLF
Sbjct: 421 LTESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLF 480

Query: 481 SGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIF 540
           SGY+KR+DLT++V IDGW HTGD+GEWQP+GSMKIIDRKKNIFKL+QGEYVAVEN+EN++
Sbjct: 481 SGYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVY 540

Query: 541 LQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDE 600
            Q  +I SIWVYGNSFESFLV +A P Q+ LE WA  + V GDF S+C+N  A+ +IL E
Sbjct: 541 SQVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGE 600

Query: 601 LNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAK 660
           L    +++ L+GFE++K +HLEP  FDMERDL+TPT+K KRPQLLKYY+  I ++Y+  K
Sbjct: 601 LVKTAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTK 660

Query: 661 KA 662
           ++
Sbjct: 661 ES 661

BLAST of CmoCh20G008840 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 650.2 bits (1676), Expect = 1.8e-186
Identity = 315/661 (47.66%), Postives = 438/661 (66.26%), Query Frame = 0

Query: 6   AYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFFSDSVKRNPGNRMLG 65
           ++   VE+      GKPS GPVYR++ ++ G   + +   + W  FS SV++ P N MLG
Sbjct: 3   SFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNMLG 62

Query: 66  RRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGSNCPEWIISMEAC 125
            RR   D K G Y+W TY+E Y+  +++GSA+R  G  PG   GIYG NCP+WII+MEAC
Sbjct: 63  WRRI-VDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEAC 122

Query: 126 NSQAITYVPLYDTLGANAVEFIINHAEVSIAFVQENKLASIFSCLPRCSTRLKTIVSFTN 185
            +  +  VPLYDTLG+ AV++I+ HAE+   FVQ+ K+  +     +C+ RLK IVSFTN
Sbjct: 123 AAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFTN 182

Query: 186 VSSVHKKEAEELGVSCYSWEEFYQLGSLDCELPSKQKT-NICTIMYTSGTTGEPKGVILN 245
           VS     +A E+GV  YSW +F  +G    E  +  K  NICTIMYTSGT+G+PKGV+L 
Sbjct: 183 VSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVLT 242

Query: 246 NAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAHVYDQIMETYCIYKGSSIGFWRGDI 305
           + A+   V+ +D  +   +   T +D Y SFLPLAH+ D++ E Y   KG+S+G++ G++
Sbjct: 243 HQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGNL 302

Query: 306 RFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLFDYAYNYKLGYLSKGVP 365
             L DDIQELKPT  +GVPRV++RI+ GI   +      R+ +F+  Y +KL +L++G  
Sbjct: 303 NVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGYS 362

Query: 366 QEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRHVEEFLRVTSCATLSQGYGLTESC 425
             KA+P+ D + F KI++  GGR+RL +SG APL   +EEFLRVT C  + QGYGLTE+ 
Sbjct: 363 HSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTETL 422

Query: 426 GGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGYDALSSTPRGEICLRGSTLFSGYHK 485
           GG      +   M+GTVG+P    E+RLE V +MGYD L   P GEIC+RG  +FSGY+K
Sbjct: 423 GGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYYK 482

Query: 486 RQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIFLQCPL 545
             +LTE+V+ DGWFHTGDIGE  P+G +KIIDRKKN+ KLSQGEYVA+E++ENIF Q  +
Sbjct: 483 NPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNSV 542

Query: 546 ITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVTGDFKSLCENLSARKYILDELNSNG 605
           +  IWVYG+SF+S LV V VP+ + +  WA++   T  F+ LC     +++I+ EL S  
Sbjct: 543 VQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKSTA 602

Query: 606 QKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYKERIDQLYEEAKKAAKP 665
           +K+ LR FE +KA+ +E  PFD+ERDL+T T K +R  LLKYY+ +ID++Y   K A+K 
Sbjct: 603 EKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYR--KLASKK 660

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
M4ISH14.4e-30776.07Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1[more]
Q9XIA96.5e-27467.62Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 S... [more]
Q9T0A03.7e-22956.77Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 S... [more]
Q9C7W45.4e-22856.40Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 S... [more]
Q9T0098.6e-22655.59Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1FW800.0e+00100.00long chain acyl-CoA synthetase 2 OS=Cucurbita moschata OX=3662 GN=LOC111449056 P... [more]
A0A6J1JE620.0e+0098.50long chain acyl-CoA synthetase 2 OS=Cucurbita maxima OX=3661 GN=LOC111484205 PE=... [more]
A0A1S3CAY40.0e+0094.25long chain acyl-CoA synthetase 2 OS=Cucumis melo OX=3656 GN=LOC103498426 PE=4 SV... [more]
A0A0A0LL210.0e+0093.19AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G238260... [more]
A0A6J1D4L40.0e+0092.54long chain acyl-CoA synthetase 2 isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_022944667.10.0e+00100.00long chain acyl-CoA synthetase 2 [Cucurbita moschata][more]
XP_023512617.10.0e+0098.80long chain acyl-CoA synthetase 2 [Cucurbita pepo subsp. pepo][more]
XP_022986475.10.0e+0098.50long chain acyl-CoA synthetase 2 [Cucurbita maxima][more]
KAG6571141.10.0e+0098.65Long chain acyl-CoA synthetase 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_008459243.10.0e+0094.25PREDICTED: long chain acyl-CoA synthetase 2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G49430.14.6e-27567.62long-chain acyl-CoA synthetase 2 [more]
AT4G23850.12.6e-23056.77AMP-dependent synthetase and ligase family protein [more]
AT1G64400.13.8e-22956.40AMP-dependent synthetase and ligase family protein [more]
AT4G11030.16.1e-22755.59AMP-dependent synthetase and ligase family protein [more]
AT2G47240.11.8e-18647.66AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 54..525
e-value: 2.5E-97
score: 326.2
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 43..517
e-value: 2.9E-86
score: 291.6
NoneNo IPR availablePANTHERPTHR43272:SF4LONG CHAIN ACYL-COA SYNTHETASE 2coord: 6..658
NoneNo IPR availablePANTHERPTHR43272LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 6..658
NoneNo IPR availableCDDcd05927LC-FACS_eukcoord: 76..655
e-value: 0.0
score: 807.209
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 46..657
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 228..239

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G008840.1CmoCh20G008840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010143 cutin biosynthetic process
biological_process GO:0001676 long-chain fatty acid metabolic process
biological_process GO:0010025 wax biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity