CmoCh20G008830 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G008830
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCmo_Chr20: 4456060 .. 4457301 (+)
RNA-Seq ExpressionCmoCh20G008830
SyntenyCmoCh20G008830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGACCGCCCCTTCTCCATCTCCATCTCCTTCTCCTTCTCCTTCCCCTTCCACCTCCTCCTCCGCTGCCGCCGCCGTCAATTTCCCTTCCCCCTCATCCTTACTGCCGGGAAAGCTCCCAGTCGACTTCAGTCCTCCTCTAATCGCAATGGTTGTCGTCATCGCTACCGCCTTTCTCATCGTCACTTACTCTCGCCTCATCTCCCGCCACGTCATCCCTCCAATCCTCCTCCTAATCCGCCGCCACCGCCGCCGCTGGCGGCGGTGGCGGCGGAGACGACGGCGCGCCCGATCTTACCTCCCGTCCTCCTCCGGCGGCGACTTGGATTCGCTCCCTTACGATTCGCCTTTCTTCGAGCATACGGATGGCTTCCACGTGTTCTCCCCGTACGGCCTCGACGAATCCATCATCAAGACGATTCCACTCTCCGTCTATACTGCCAAGAGCCGCCGTGACAGCCGTGACCGTGAGTGCGCCGTTTGTCTTCTGGAGTTTGAAGACGACGATTACGTCCGAACGCTTCCGATTTGTTCTCACGCATTTCACGTTGATTGTATAGACGTATGGCTTAGATCACACGCGAACTGTCCTCTCTGTCGCGCGGTGGTTTTCAGGCCAGAATCTCCGTTCACGCCGGTTATGGCAGCAAGAATCCGGCCAAGCCTCGACGAAACGATCTTACAGAGCATCGTATTAGAGCCGCTAGCGGAAGGTCCTATTCCTAATCGAGATTCTTATCACGCAACAGAATCGGAAATCACGCCTTGCGTTGATGAACCAAGGAACAGTAATTCGTTAGAGGATCAAATGAACGGGCGAGATTTCTTACTGAAACGATCGTATTCATTCGGATTCGAACGAAGCTTGGCGTCAGAGAGAATGATTCTGGAACCAGCAACCGCATCGCCATGGAGGTACCGCCGTGGAAGCTTCTGGAGCAAACGGCCATCACCGTTCGGATCTCTACCGAAGGCGAGAGTGTTCTCATTCCGATACTACAGAGGAATGAAATCGCCGTTCTTCAGACGACGAGGAGGATTCTTTCCGCTATCGGAATCAAGCTGGAGATTCTCCAACGGCGTAGGCGGAGGTTCATCGAGACGAAGCAAGTCGATGACAAGTCCAATGTTTCTCCGATCGTCGGTAACCAGCGGAGGAGCAGCAACGTTCTCATCGAGCCGATTGAGATGCGGAGATCCCGAGGCATTACTATCACCGGAGAGATTCAACCGAAGATGA

mRNA sequence

ATGTCGACCGCCCCTTCTCCATCTCCATCTCCTTCTCCTTCTCCTTCCCCTTCCACCTCCTCCTCCGCTGCCGCCGCCGTCAATTTCCCTTCCCCCTCATCCTTACTGCCGGGAAAGCTCCCAGTCGACTTCAGTCCTCCTCTAATCGCAATGGTTGTCGTCATCGCTACCGCCTTTCTCATCGTCACTTACTCTCGCCTCATCTCCCGCCACGTCATCCCTCCAATCCTCCTCCTAATCCGCCGCCACCGCCGCCGCTGGCGGCGGTGGCGGCGGAGACGACGGCGCGCCCGATCTTACCTCCCGTCCTCCTCCGGCGGCGACTTGGATTCGCTCCCTTACGATTCGCCTTTCTTCGAGCATACGGATGGCTTCCACGTGTTCTCCCCGTACGGCCTCGACGAATCCATCATCAAGACGATTCCACTCTCCGTCTATACTGCCAAGAGCCGCCGTGACAGCCGTGACCGTGAGTGCGCCGTTTGTCTTCTGGAGTTTGAAGACGACGATTACGTCCGAACGCTTCCGATTTGTTCTCACGCATTTCACGTTGATTGTATAGACGTATGGCTTAGATCACACGCGAACTGTCCTCTCTGTCGCGCGGTGGTTTTCAGGCCAGAATCTCCGTTCACGCCGGTTATGGCAGCAAGAATCCGGCCAAGCCTCGACGAAACGATCTTACAGAGCATCGTATTAGAGCCGCTAGCGGAAGGTCCTATTCCTAATCGAGATTCTTATCACGCAACAGAATCGGAAATCACGCCTTGCGTTGATGAACCAAGGAACAGTAATTCGTTAGAGGATCAAATGAACGGGCGAGATTTCTTACTGAAACGATCGTATTCATTCGGATTCGAACGAAGCTTGGCGTCAGAGAGAATGATTCTGGAACCAGCAACCGCATCGCCATGGAGGTACCGCCGTGGAAGCTTCTGGAGCAAACGGCCATCACCGTTCGGATCTCTACCGAAGGCGAGAGTGTTCTCATTCCGATACTACAGAGGAATGAAATCGCCGTTCTTCAGACGACGAGGAGGATTCTTTCCGCTATCGGAATCAAGCTGGAGATTCTCCAACGGCGTAGGCGGAGGTTCATCGAGACGAAGCAAGTCGATGACAAGTCCAATGTTTCTCCGATCGTCGGTAACCAGCGGAGGAGCAGCAACGTTCTCATCGAGCCGATTGAGATGCGGAGATCCCGAGGCATTACTATCACCGGAGAGATTCAACCGAAGATGA

Coding sequence (CDS)

ATGTCGACCGCCCCTTCTCCATCTCCATCTCCTTCTCCTTCTCCTTCCCCTTCCACCTCCTCCTCCGCTGCCGCCGCCGTCAATTTCCCTTCCCCCTCATCCTTACTGCCGGGAAAGCTCCCAGTCGACTTCAGTCCTCCTCTAATCGCAATGGTTGTCGTCATCGCTACCGCCTTTCTCATCGTCACTTACTCTCGCCTCATCTCCCGCCACGTCATCCCTCCAATCCTCCTCCTAATCCGCCGCCACCGCCGCCGCTGGCGGCGGTGGCGGCGGAGACGACGGCGCGCCCGATCTTACCTCCCGTCCTCCTCCGGCGGCGACTTGGATTCGCTCCCTTACGATTCGCCTTTCTTCGAGCATACGGATGGCTTCCACGTGTTCTCCCCGTACGGCCTCGACGAATCCATCATCAAGACGATTCCACTCTCCGTCTATACTGCCAAGAGCCGCCGTGACAGCCGTGACCGTGAGTGCGCCGTTTGTCTTCTGGAGTTTGAAGACGACGATTACGTCCGAACGCTTCCGATTTGTTCTCACGCATTTCACGTTGATTGTATAGACGTATGGCTTAGATCACACGCGAACTGTCCTCTCTGTCGCGCGGTGGTTTTCAGGCCAGAATCTCCGTTCACGCCGGTTATGGCAGCAAGAATCCGGCCAAGCCTCGACGAAACGATCTTACAGAGCATCGTATTAGAGCCGCTAGCGGAAGGTCCTATTCCTAATCGAGATTCTTATCACGCAACAGAATCGGAAATCACGCCTTGCGTTGATGAACCAAGGAACAGTAATTCGTTAGAGGATCAAATGAACGGGCGAGATTTCTTACTGAAACGATCGTATTCATTCGGATTCGAACGAAGCTTGGCGTCAGAGAGAATGATTCTGGAACCAGCAACCGCATCGCCATGGAGGTACCGCCGTGGAAGCTTCTGGAGCAAACGGCCATCACCGTTCGGATCTCTACCGAAGGCGAGAGTGTTCTCATTCCGATACTACAGAGGAATGAAATCGCCGTTCTTCAGACGACGAGGAGGATTCTTTCCGCTATCGGAATCAAGCTGGAGATTCTCCAACGGCGTAGGCGGAGGTTCATCGAGACGAAGCAAGTCGATGACAAGTCCAATGTTTCTCCGATCGTCGGTAACCAGCGGAGGAGCAGCAACGTTCTCATCGAGCCGATTGAGATGCGGAGATCCCGAGGCATTACTATCACCGGAGAGATTCAACCGAAGATGA

Protein sequence

MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFLIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFEHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGPIPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSNGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR
Homology
BLAST of CmoCh20G008830 vs. ExPASy Swiss-Prot
Match: Q67YI6 (RING-H2 finger protein ATL65 OS=Arabidopsis thaliana OX=3702 GN=ATL65 PE=2 SV=2)

HSP 1 Score: 325.9 bits (834), Expect = 6.8e-88
Identity = 226/440 (51.36%), Postives = 275/440 (62.50%), Query Frame = 0

Query: 4   APSP-SPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFLIV 63
           AP P S   SPSPSPS+  S         P        P++FSPPLIAMVVV+A AFL V
Sbjct: 5   APPPRSGDNSPSPSPSSGISEEILSRSSDP--------PLEFSPPLIAMVVVLAAAFLFV 64

Query: 64  TYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRR----ARSYLPSSSGGDLDSLPYDSPF 123
           TYSRLISR  + P+        RR+RRWR RRRR    + +   S+S  DL S    SPF
Sbjct: 65  TYSRLISRRFLSPLF-------RRFRRWRCRRRRLLHLSSASSASTSSSDLRSF---SPF 124

Query: 124 FEHTDGFHV--FSPYGLDESIIKTIPLSVYTAKS---------RRDSRDRECAVCLLEFE 183
               D FH   +SPYGLD+S+IKT+PL +Y+A +            +  R+CAVCLLEFE
Sbjct: 125 --PFDSFHYSSYSPYGLDDSVIKTLPLFLYSAAACTGKPAVGKTSAANCRDCAVCLLEFE 184

Query: 184 DDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCR------AVVFRPESPFTPVMAARIRP 243
           + DYVRTLP+C HAFH++CID WLRSH NCPLCR      A V  P SPF P+MA RIRP
Sbjct: 185 EGDYVRTLPLCFHAFHLECIDEWLRSHPNCPLCRTAILGSAGVLTPMSPFVPLMAPRIRP 244

Query: 244 SLDETILQSIVLEPLAEGPI-PNRDSYHATESEITPCVDEPRNSNSLEDQMN---GRDFL 303
           SLD+    +I++     G I P+R +++   ++ T   ++     S+E+Q +    R   
Sbjct: 245 SLDDEENNAIII----RGEITPSRSNWNTIAADTT---NDQEIRASVEEQSSPAISRFRE 304

Query: 304 LKRSYSFGFERSLASERMILEPATASPWRYRRGSFWSKRPSPFGSL-PKARVFSFRYYRG 363
           LKRSYSF  ER   SER+ +EPAT SPWRYRR S W+KR SPFG+L  K+RVFSFRYYR 
Sbjct: 305 LKRSYSFECERESESERVTMEPATVSPWRYRR-STWNKRQSPFGNLISKSRVFSFRYYRS 364

Query: 364 MKSPFFRRR---GGFFPLSESSWRFSNGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSS 414
            KSPFFRRR   G F+P+SE          G SSRR+KSMTSPMF R+       A  SS
Sbjct: 365 TKSPFFRRRSSAGVFYPISERI-----PATGSSSRRTKSMTSPMFFRT-------APHSS 404

BLAST of CmoCh20G008830 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 97.8 bits (242), Expect = 3.0e-19
Identity = 62/171 (36.26%), Postives = 92/171 (53.80%), Query Frame = 0

Query: 43  DFSPPLIAMVVVIATAFLIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLP 102
           DFSP LIA++ ++A+AF++V+Y  LIS++                      RRR  S   
Sbjct: 55  DFSPLLIALIGILASAFILVSYYTLISKYC--------------------HRRRHNSSST 114

Query: 103 SSSGGDLDSLPYDSPFFEHTDGFHVFSPY---------GLDESIIKTIPLSVYTAKSRRD 162
           S++  +  S  Y      + +     +P          GLDES+IK+I +  Y  K    
Sbjct: 115 SAAAINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKY-RKMDGF 174

Query: 163 SRDRECAVCLLEFEDDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVV 205
               +C+VCL EF++++ +R LP C+HAFHV CID WL+SH+NCPLCRA +
Sbjct: 175 VESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFI 204

BLAST of CmoCh20G008830 vs. ExPASy Swiss-Prot
Match: Q9LY41 (E3 ubiquitin-protein ligase ATL4 OS=Arabidopsis thaliana OX=3702 GN=ATL4 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.1e-18
Identity = 49/121 (40.50%), Postives = 71/121 (58.68%), Query Frame = 0

Query: 98  RSYLPSSSGGDLDSLPYDSPFFEHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDS--R 157
           RS LP SS   + ++  DS  F      H  SP     S++ ++P+  +++ +RR S   
Sbjct: 58  RSVLPLSSSSSVATVTSDSRRFSG----HRVSPETERSSVLDSLPIFKFSSVTRRSSSMN 117

Query: 158 DRECAVCLLEFEDDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVM 217
             +CAVCL +FE +D +R LP+C HAFH DCID+WL S+  CPLCR+ +F  ES     +
Sbjct: 118 SGDCAVCLSKFEPEDQLRLLPLCCHAFHADCIDIWLVSNQTCPLCRSPLFASESDLMKSL 174

BLAST of CmoCh20G008830 vs. ExPASy Swiss-Prot
Match: Q9LZJ6 (RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.5e-18
Identity = 71/193 (36.79%), Postives = 97/193 (50.26%), Query Frame = 0

Query: 76  ILLLIRRHRRRW---RRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFEHTDGFHVFSPYG 135
           IL+L      RW   R+ RR RRR  ++L S +              + T      SP  
Sbjct: 41  ILILCFHSYARWLFRRQNRRIRRRISAHLRSLAAAR-----------DPTQSSSSLSP-- 100

Query: 136 LDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSHAFHVDCIDVWLR 195
           LD ++++ IP+ VY+ K+  +S   EC+VCL EFE+DD  R LP C H FHVDCID W R
Sbjct: 101 LDPTVLEKIPIFVYSVKT-HESPLEECSVCLSEFEEDDEGRVLPKCGHVFHVDCIDTWFR 160

Query: 196 SHANCPLCRAVVFRPESPFT-PVMAARIRPS---LDETILQSIVLEPLAEGPIPNRDSYH 255
           S ++CPLCRA V +P  P T P   A + PS   +++T   S      +E   P+  S  
Sbjct: 161 SRSSCPLCRAPV-QPAQPVTEPEPVAAVFPSVKPIEDTEAGSSSSSDESESSTPSSSSGS 218

Query: 256 ATESEITPCVDEP 262
                +  C  EP
Sbjct: 221 PVRFPMEACEREP 218

BLAST of CmoCh20G008830 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 2.4e-16
Identity = 61/161 (37.89%), Postives = 85/161 (52.80%), Query Frame = 0

Query: 44  FSPPLIAMVVVIATAFLIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPS 103
           FSP +IA++ V+A+AFL+V+Y   IS++      L            R R   + S   +
Sbjct: 55  FSPLVIAIIGVLASAFLLVSYYTFISKYCGTVSSL------------RGRVFGSSSGGAA 114

Query: 104 SSGGDLDSLPYDSPFFEHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDS--RDRECAV 163
             GG      +        + ++V  P GLDE++I  I +  Y    R D      +C+V
Sbjct: 115 YGGGAGSGGRHGHGQSRSHESWNVSPPSGLDETLINKITVCKY---RRGDGFVHTTDCSV 174

Query: 164 CLLEFEDDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRA 203
           CL EF D + +R LP CSHAFH  CID WL+SH+NCPLCRA
Sbjct: 175 CLGEFSDGESLRLLPRCSHAFHQQCIDTWLKSHSNCPLCRA 200

BLAST of CmoCh20G008830 vs. ExPASy TrEMBL
Match: A0A6J1FVL7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448502 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 6.3e-230
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60
           MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120
           IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120

Query: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180
           HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH
Sbjct: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180

Query: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP 240
           AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP
Sbjct: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP 240

Query: 241 IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300
           IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA
Sbjct: 241 IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300

Query: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360
           TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN
Sbjct: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360

Query: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 414
           GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of CmoCh20G008830 vs. ExPASy TrEMBL
Match: A0A6J1JBG1 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111484253 PE=3 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 1.8e-224
Identity = 405/413 (98.06%), Postives = 409/413 (99.03%), Query Frame = 0

Query: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60
           MSTAPSPSPSPSPSPS S+SSS+AAAVNFP PSSLLP KLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSTSSSSSSAAAVNFPPPSSLLPAKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120
           IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120

Query: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180
           HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH
Sbjct: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180

Query: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP 240
           AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TILQSIVLEPLAEGP
Sbjct: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILQSIVLEPLAEGP 240

Query: 241 IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300
           IPNRDSY+ATESEITP VDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA
Sbjct: 241 IPNRDSYNATESEITPYVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300

Query: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360
           TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN
Sbjct: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360

Query: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 414
           GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of CmoCh20G008830 vs. ExPASy TrEMBL
Match: A0A0A0LIL3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G238760 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 7.5e-207
Identity = 385/421 (91.45%), Postives = 396/421 (94.06%), Query Frame = 0

Query: 1   MSTAPSPSPSPSPSPSPST----SSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIA 60
           M T+PSPSPSPSPSPSPST    SSS+AAA NFP PSSL P KLPVDFSPPLIAMVVVIA
Sbjct: 1   MPTSPSPSPSPSPSPSPSTSSSSSSSSAAAANFPPPSSLQPAKLPVDFSPPLIAMVVVIA 60

Query: 61  TAFLIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRR-RARSYLPSSSGGDLDSLPYD 120
           TAFLIVTYSRLISRH+IPPIL L+R HRRRWRRWRRRRR  ARSY+PSSSGGDLDSLPYD
Sbjct: 61  TAFLIVTYSRLISRHLIPPILHLLRLHRRRWRRWRRRRRLAARSYIPSSSGGDLDSLPYD 120

Query: 121 SPFFE-HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRT 180
           SPFFE  TD FHVFSPYGLDES+IKTIPLSVYTAK    SRDRECAVCLLEFEDDDYVRT
Sbjct: 121 SPFFEPTTDAFHVFSPYGLDESVIKTIPLSVYTAK----SRDRECAVCLLEFEDDDYVRT 180

Query: 181 LPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLE 240
           LPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TIL+SI+LE
Sbjct: 181 LPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIILE 240

Query: 241 PLAEG-PIPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASE 300
           PLAEG PIPNRDSYHATESEITPC+DEPRNSNS+EDQMNGRDFLLKRSYSFGFERSLASE
Sbjct: 241 PLAEGPPIPNRDSYHATESEITPCIDEPRNSNSVEDQMNGRDFLLKRSYSFGFERSLASE 300

Query: 301 RMILEPATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSE 360
           RMILEPAT SPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSE
Sbjct: 301 RMILEPATTSPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSE 360

Query: 361 SSWRFSNGVGGGSSRRSKSMTSPMFLRSSVT-SGGAATFSSSRLRCGDPEALLSPERFNR 414
           SSWRFSNGVGGGSSRRSKSMTSPMFLRSSVT  GGAA FSSSRLRCGDPEALLSPERFNR
Sbjct: 361 SSWRFSNGVGGGSSRRSKSMTSPMFLRSSVTGGGGAAAFSSSRLRCGDPEALLSPERFNR 417

BLAST of CmoCh20G008830 vs. ExPASy TrEMBL
Match: A0A1S4E2C5 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498427 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 8.6e-203
Identity = 375/410 (91.46%), Postives = 385/410 (93.90%), Query Frame = 0

Query: 10  SPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFLIVTYSRLIS 69
           SPSPSPS S S+SAAAA NFP PSSL   KLPVDFSPPLIAMVVVIATAFLIVTYSRLIS
Sbjct: 4   SPSPSPSLSPSTSAAAAANFPPPSSLQSAKLPVDFSPPLIAMVVVIATAFLIVTYSRLIS 63

Query: 70  RHVIPPILLLIRRHRRRWRRWRRRRRR-ARSYLPSSSGGDLDSLPYDSPFFE-HTDGFHV 129
           RH++PPIL L+R HRRRWRRWRRRRRR ARSY+PSSSGGDLDSLPYDSPFFE  TD FHV
Sbjct: 64  RHLVPPILHLLRLHRRRWRRWRRRRRRAARSYIPSSSGGDLDSLPYDSPFFEPTTDAFHV 123

Query: 130 FSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSHAFHVDCI 189
           FSPYGLDES+IKTIPLSVYTAK    SRDRECAVCLLEFEDDDYVRTLPICSHAFHVDCI
Sbjct: 124 FSPYGLDESVIKTIPLSVYTAK----SRDRECAVCLLEFEDDDYVRTLPICSHAFHVDCI 183

Query: 190 DVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP-IPNRDS 249
           DVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TIL+SIVLEPLAEGP IPNRDS
Sbjct: 184 DVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLAEGPLIPNRDS 243

Query: 250 YHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPATASPWR 309
           YHATESEITPC+DEPRNSNS+EDQMNGRDFLLKRSYSFGFERSLASERMILEPAT SPWR
Sbjct: 244 YHATESEITPCIDEPRNSNSVEDQMNGRDFLLKRSYSFGFERSLASERMILEPATTSPWR 303

Query: 310 YRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSNGVGGGS 369
           YRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSNGVGGGS
Sbjct: 304 YRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSNGVGGGS 363

Query: 370 SRRSKSMTSPMFLRSSVT---SGGAATFSSSRLRCGDPEALLSPERFNRR 414
           SRRSKSMTSPMFLRSSVT    GG A FSSSRLRCGDPEALLSPERFNRR
Sbjct: 364 SRRSKSMTSPMFLRSSVTGGGGGGGAAFSSSRLRCGDPEALLSPERFNRR 409

BLAST of CmoCh20G008830 vs. ExPASy TrEMBL
Match: A0A5A7TSC8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G005120 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.7e-188
Identity = 343/368 (93.21%), Postives = 351/368 (95.38%), Query Frame = 0

Query: 51  MVVVIATAFLIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRR-ARSYLPSSSGGDL 110
           MVVVIATAFLIVTYSRLISRH+IPPIL L+R HRRRWRRWRRRRRR ARSY+PSSSGGDL
Sbjct: 1   MVVVIATAFLIVTYSRLISRHLIPPILHLLRLHRRRWRRWRRRRRRAARSYIPSSSGGDL 60

Query: 111 DSLPYDSPFFE-HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFED 170
           DSLPYDSPFFE  TD FHVFSPYGLDES+IKTIPLSVYTAK    SRDRECAVCLLEFED
Sbjct: 61  DSLPYDSPFFEPTTDAFHVFSPYGLDESVIKTIPLSVYTAK----SRDRECAVCLLEFED 120

Query: 171 DDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETIL 230
           DDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TIL
Sbjct: 121 DDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTIL 180

Query: 231 QSIVLEPLAEGP-IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFE 290
           +SIVLEPLAEGP IPNRDSYHATESEITPC+DEPRNSNS+EDQMNGRDFLLKRSYSFGFE
Sbjct: 181 RSIVLEPLAEGPLIPNRDSYHATESEITPCIDEPRNSNSVEDQMNGRDFLLKRSYSFGFE 240

Query: 291 RSLASERMILEPATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGG 350
           RSLASERMILEPAT SPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGG
Sbjct: 241 RSLASERMILEPATTSPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGG 300

Query: 351 FFPLSESSWRFSNGVGGGSSRRSKSMTSPMFLRSSVT--SGGAATFSSSRLRCGDPEALL 410
           FFPLSESSWRFSNGVGGGSSRRSKSMTSPMFLRSSVT   GG A FSSSRLRCGDPEALL
Sbjct: 301 FFPLSESSWRFSNGVGGGSSRRSKSMTSPMFLRSSVTGGGGGGAAFSSSRLRCGDPEALL 360

Query: 411 SPERFNRR 414
           SPERFNRR
Sbjct: 361 SPERFNRR 364

BLAST of CmoCh20G008830 vs. NCBI nr
Match: XP_022943929.1 (RING-H2 finger protein ATL65 [Cucurbita moschata] >KAG7010952.1 RING-H2 finger protein ATL65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 806.2 bits (2081), Expect = 1.3e-229
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60
           MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120
           IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120

Query: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180
           HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH
Sbjct: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180

Query: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP 240
           AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP
Sbjct: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP 240

Query: 241 IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300
           IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA
Sbjct: 241 IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300

Query: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360
           TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN
Sbjct: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360

Query: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 414
           GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of CmoCh20G008830 vs. NCBI nr
Match: KAG6571140.1 (RING-H2 finger protein ATL65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 802.7 bits (2072), Expect = 1.4e-228
Identity = 411/413 (99.52%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60
           MSTAPSPSPSPSPSPSPSTSSS AA VNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSPSTSSSTAAPVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120
           IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120

Query: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180
           HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH
Sbjct: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180

Query: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP 240
           AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP
Sbjct: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP 240

Query: 241 IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300
           IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA
Sbjct: 241 IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300

Query: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360
           TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN
Sbjct: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360

Query: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 414
           GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of CmoCh20G008830 vs. NCBI nr
Match: XP_023511773.1 (RING-H2 finger protein ATL65 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 793.1 bits (2047), Expect = 1.1e-225
Identity = 408/414 (98.55%), Postives = 411/414 (99.28%), Query Frame = 0

Query: 1   MSTAPSPSPSPSPSPSPSTSSSA-AAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAF 60
           MSTAPSPSPS SPSPSPSTSSS+ AAAVNFP PSSLLPGKLPVDFSPPLIAMVVVIATAF
Sbjct: 1   MSTAPSPSPSSSPSPSPSTSSSSYAAAVNFPPPSSLLPGKLPVDFSPPLIAMVVVIATAF 60

Query: 61  LIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFF 120
           LIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFF
Sbjct: 61  LIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFF 120

Query: 121 EHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICS 180
           EHTDG+HVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICS
Sbjct: 121 EHTDGYHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICS 180

Query: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEG 240
           HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TILQSIVLEPLAEG
Sbjct: 181 HAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILQSIVLEPLAEG 240

Query: 241 PIPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEP 300
           PIPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEP
Sbjct: 241 PIPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEP 300

Query: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFS 360
           ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFS
Sbjct: 301 ATASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFS 360

Query: 361 NGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 414
           NGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 NGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 414

BLAST of CmoCh20G008830 vs. NCBI nr
Match: XP_022986546.1 (RING-H2 finger protein ATL65 [Cucurbita maxima])

HSP 1 Score: 788.1 bits (2034), Expect = 3.7e-224
Identity = 405/413 (98.06%), Postives = 409/413 (99.03%), Query Frame = 0

Query: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60
           MSTAPSPSPSPSPSPS S+SSS+AAAVNFP PSSLLP KLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MSTAPSPSPSPSPSPSTSSSSSSAAAVNFPPPSSLLPAKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120
           IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE
Sbjct: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120

Query: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180
           HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH
Sbjct: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180

Query: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP 240
           AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TILQSIVLEPLAEGP
Sbjct: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILQSIVLEPLAEGP 240

Query: 241 IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300
           IPNRDSY+ATESEITP VDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA
Sbjct: 241 IPNRDSYNATESEITPYVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300

Query: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360
           TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN
Sbjct: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360

Query: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 414
           GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 413

BLAST of CmoCh20G008830 vs. NCBI nr
Match: XP_038902895.1 (RING-H2 finger protein ATL65 [Benincasa hispida])

HSP 1 Score: 736.5 bits (1900), Expect = 1.3e-208
Identity = 379/413 (91.77%), Postives = 389/413 (94.19%), Query Frame = 0

Query: 1   MSTAPSPSPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFL 60
           M TAPSPSPSPSP       S+AA+A NFP PSSL P KLPVDFSPPLIAMVVVIATAFL
Sbjct: 1   MPTAPSPSPSPSP-------STAASAANFPPPSSLQPAKLPVDFSPPLIAMVVVIATAFL 60

Query: 61  IVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120
           IVTYSRLISRH+IPPIL L+R HRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE
Sbjct: 61  IVTYSRLISRHLIPPILHLLRLHRRRWRRWRRRRRRARSYLPSSSGGDLDSLPYDSPFFE 120

Query: 121 HTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFEDDDYVRTLPICSH 180
            TDGFHVFSPYGLDES+IKTIPLSVYTAK    SRDRECAVCLLEFEDDDYVRTLPICSH
Sbjct: 121 PTDGFHVFSPYGLDESVIKTIPLSVYTAK----SRDRECAVCLLEFEDDDYVRTLPICSH 180

Query: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDETILQSIVLEPLAEGP 240
           AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLD+TIL+SIVLEPL EG 
Sbjct: 181 AFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRPSLDDTILRSIVLEPLVEGT 240

Query: 241 IPNRDSYHATESEITPCVDEPRNSNSLEDQMNGRDFLLKRSYSFGFERSLASERMILEPA 300
           IPNRDSYHATESEITPCVDEPRNSNS++DQ+NGRDFLLKRSYSFGFERSLASERMILEPA
Sbjct: 241 IPNRDSYHATESEITPCVDEPRNSNSVDDQINGRDFLLKRSYSFGFERSLASERMILEPA 300

Query: 301 TASPWRYRRGSFWSKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360
           T SPWRYRRGSFW KRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN
Sbjct: 301 TTSPWRYRRGSFWMKRPSPFGSLPKARVFSFRYYRGMKSPFFRRRGGFFPLSESSWRFSN 360

Query: 361 GVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSSSRLRCGDPEALLSPERFNRR 414
           GVGGGSSRRSKSMTSPMFLRSSVT GGAATFSSSRLRCGDPEALLSPERFNRR
Sbjct: 361 GVGGGSSRRSKSMTSPMFLRSSVTGGGAATFSSSRLRCGDPEALLSPERFNRR 402

BLAST of CmoCh20G008830 vs. TAIR 10
Match: AT3G18930.1 (RING/U-box superfamily protein )

HSP 1 Score: 325.9 bits (834), Expect = 4.8e-89
Identity = 226/440 (51.36%), Postives = 275/440 (62.50%), Query Frame = 0

Query: 4   APSP-SPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFLIV 63
           AP P S   SPSPSPS+  S         P        P++FSPPLIAMVVV+A AFL V
Sbjct: 5   APPPRSGDNSPSPSPSSGISEEILSRSSDP--------PLEFSPPLIAMVVVLAAAFLFV 64

Query: 64  TYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRR----ARSYLPSSSGGDLDSLPYDSPF 123
           TYSRLISR  + P+        RR+RRWR RRRR    + +   S+S  DL S    SPF
Sbjct: 65  TYSRLISRRFLSPLF-------RRFRRWRCRRRRLLHLSSASSASTSSSDLRSF---SPF 124

Query: 124 FEHTDGFHV--FSPYGLDESIIKTIPLSVYTAKS---------RRDSRDRECAVCLLEFE 183
               D FH   +SPYGLD+S+IKT+PL +Y+A +            +  R+CAVCLLEFE
Sbjct: 125 --PFDSFHYSSYSPYGLDDSVIKTLPLFLYSAAACTGKPAVGKTSAANCRDCAVCLLEFE 184

Query: 184 DDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCR------AVVFRPESPFTPVMAARIRP 243
           + DYVRTLP+C HAFH++CID WLRSH NCPLCR      A V  P SPF P+MA RIRP
Sbjct: 185 EGDYVRTLPLCFHAFHLECIDEWLRSHPNCPLCRTAILGSAGVLTPMSPFVPLMAPRIRP 244

Query: 244 SLDETILQSIVLEPLAEGPI-PNRDSYHATESEITPCVDEPRNSNSLEDQMN---GRDFL 303
           SLD+    +I++     G I P+R +++   ++ T   ++     S+E+Q +    R   
Sbjct: 245 SLDDEENNAIII----RGEITPSRSNWNTIAADTT---NDQEIRASVEEQSSPAISRFRE 304

Query: 304 LKRSYSFGFERSLASERMILEPATASPWRYRRGSFWSKRPSPFGSL-PKARVFSFRYYRG 363
           LKRSYSF  ER   SER+ +EPAT SPWRYRR S W+KR SPFG+L  K+RVFSFRYYR 
Sbjct: 305 LKRSYSFECERESESERVTMEPATVSPWRYRR-STWNKRQSPFGNLISKSRVFSFRYYRS 364

Query: 364 MKSPFFRRR---GGFFPLSESSWRFSNGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSS 414
            KSPFFRRR   G F+P+SE          G SSRR+KSMTSPMF R+       A  SS
Sbjct: 365 TKSPFFRRRSSAGVFYPISERI-----PATGSSSRRTKSMTSPMFFRT-------APHSS 404

BLAST of CmoCh20G008830 vs. TAIR 10
Match: AT3G18930.2 (RING/U-box superfamily protein )

HSP 1 Score: 325.9 bits (834), Expect = 4.8e-89
Identity = 226/440 (51.36%), Postives = 275/440 (62.50%), Query Frame = 0

Query: 4   APSP-SPSPSPSPSPSTSSSAAAAVNFPSPSSLLPGKLPVDFSPPLIAMVVVIATAFLIV 63
           AP P S   SPSPSPS+  S         P        P++FSPPLIAMVVV+A AFL V
Sbjct: 5   APPPRSGDNSPSPSPSSGISEEILSRSSDP--------PLEFSPPLIAMVVVLAAAFLFV 64

Query: 64  TYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRR----ARSYLPSSSGGDLDSLPYDSPF 123
           TYSRLISR  + P+        RR+RRWR RRRR    + +   S+S  DL S    SPF
Sbjct: 65  TYSRLISRRFLSPLF-------RRFRRWRCRRRRLLHLSSASSASTSSSDLRSF---SPF 124

Query: 124 FEHTDGFHV--FSPYGLDESIIKTIPLSVYTAKS---------RRDSRDRECAVCLLEFE 183
               D FH   +SPYGLD+S+IKT+PL +Y+A +            +  R+CAVCLLEFE
Sbjct: 125 --PFDSFHYSSYSPYGLDDSVIKTLPLFLYSAAACTGKPAVGKTSAANCRDCAVCLLEFE 184

Query: 184 DDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCR------AVVFRPESPFTPVMAARIRP 243
           + DYVRTLP+C HAFH++CID WLRSH NCPLCR      A V  P SPF P+MA RIRP
Sbjct: 185 EGDYVRTLPLCFHAFHLECIDEWLRSHPNCPLCRTAILGSAGVLTPMSPFVPLMAPRIRP 244

Query: 244 SLDETILQSIVLEPLAEGPI-PNRDSYHATESEITPCVDEPRNSNSLEDQMN---GRDFL 303
           SLD+    +I++     G I P+R +++   ++ T   ++     S+E+Q +    R   
Sbjct: 245 SLDDEENNAIII----RGEITPSRSNWNTIAADTT---NDQEIRASVEEQSSPAISRFRE 304

Query: 304 LKRSYSFGFERSLASERMILEPATASPWRYRRGSFWSKRPSPFGSL-PKARVFSFRYYRG 363
           LKRSYSF  ER   SER+ +EPAT SPWRYRR S W+KR SPFG+L  K+RVFSFRYYR 
Sbjct: 305 LKRSYSFECERESESERVTMEPATVSPWRYRR-STWNKRQSPFGNLISKSRVFSFRYYRS 364

Query: 364 MKSPFFRRR---GGFFPLSESSWRFSNGVGGGSSRRSKSMTSPMFLRSSVTSGGAATFSS 414
            KSPFFRRR   G F+P+SE          G SSRR+KSMTSPMF R+       A  SS
Sbjct: 365 TKSPFFRRRSSAGVFYPISERI-----PATGSSSRRTKSMTSPMFFRT-------APHSS 404

BLAST of CmoCh20G008830 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 97.8 bits (242), Expect = 2.1e-20
Identity = 62/171 (36.26%), Postives = 92/171 (53.80%), Query Frame = 0

Query: 43  DFSPPLIAMVVVIATAFLIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLP 102
           DFSP LIA++ ++A+AF++V+Y  LIS++                      RRR  S   
Sbjct: 55  DFSPLLIALIGILASAFILVSYYTLISKYC--------------------HRRRHNSSST 114

Query: 103 SSSGGDLDSLPYDSPFFEHTDGFHVFSPY---------GLDESIIKTIPLSVYTAKSRRD 162
           S++  +  S  Y      + +     +P          GLDES+IK+I +  Y  K    
Sbjct: 115 SAAAINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKY-RKMDGF 174

Query: 163 SRDRECAVCLLEFEDDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVV 205
               +C+VCL EF++++ +R LP C+HAFHV CID WL+SH+NCPLCRA +
Sbjct: 175 VESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFI 204

BLAST of CmoCh20G008830 vs. TAIR 10
Match: AT3G16720.1 (TOXICOS EN LEVADURA 2 )

HSP 1 Score: 87.8 bits (216), Expect = 2.2e-17
Identity = 74/260 (28.46%), Postives = 123/260 (47.31%), Query Frame = 0

Query: 48  LIAMVVVIATAFLIVTYSRLISRHVIPPILLLIRRHRRRWRRWRRRRRRARSYLPSSSGG 107
           +++ +V++    +++ +  L +R        L+R  RR  RR  R RR    +  +    
Sbjct: 31  MLSAIVILFFVVILMVFLHLYAR------WYLLRARRRHLRRRSRNRRATMVFFTADPS- 90

Query: 108 DLDSLPYDSPFFEHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLLEFE 167
                         T    V +  GLD ++IK++P+  ++ ++ +D    ECAVCL EFE
Sbjct: 91  --------------TAATSVVASRGLDPNVIKSLPVFTFSDETHKD--PIECAVCLSEFE 150

Query: 168 DDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAARIRP---SLD 227
           + +  R LP C H FHVDCID+W  SH+ CPLCR++V    +      AAR R    ++D
Sbjct: 151 ESETGRVLPNCQHTFHVDCIDMWFHSHSTCPLCRSLV-ESLAGIESTAAAREREVVIAVD 210

Query: 228 ETILQSIVLEP-----LAEGPIPNRDSYHATE--SEITPCVDEPRNS-NSLEDQMNGRDF 287
              +  +V+EP     L + P  +  S    E        ++ PR + +  ED++  RD 
Sbjct: 211 SDPV--LVIEPSSSSGLTDEPHGSGSSQMLREDSGRKPAAIEVPRRTFSEFEDELTRRDS 264

Query: 288 LLKRSYSFGFERSLASERMI 297
              +S+     R L+  RM+
Sbjct: 271 PASQSFRSPMSRMLSFTRML 264

BLAST of CmoCh20G008830 vs. TAIR 10
Match: AT5G58580.1 (TOXICOS EN LEVADURA 63 )

HSP 1 Score: 85.9 bits (211), Expect = 8.4e-17
Identity = 60/160 (37.50%), Postives = 76/160 (47.50%), Query Frame = 0

Query: 48  LIAMVVVIATAFLIVTYSRLISRHVIPPI---LLLIRRHRRRWRRWRRRRRRARSYLPSS 107
           L+A +V +    L V      +R    P         RHRRR RR RRR       +PS 
Sbjct: 30  LLAALVFLLLVVLFVLLLHFYARFFWSPSHQDFSAAARHRRRRRRNRRRTVTTTRIIPSL 89

Query: 108 SGGDLDSLPYDSPFFEHTDGFHVFSPYGLDESIIKTIPLSVYTAKSRRDSRDRECAVCLL 167
             G  D     SP    T         GLD S+I +IPL VY      +  + EC +CL 
Sbjct: 90  PLGGFDD-GVSSPAATATR-----DDKGLDSSVISSIPLFVYEENEEEEDEEEECVICLG 149

Query: 168 EFEDDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVV 205
            +E  D+ R L  C H FHV+CID+WL SH+ CPLCR+ V
Sbjct: 150 LWEAGDFGRKLRNCGHGFHVECIDMWLSSHSTCPLCRSPV 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q67YI66.8e-8851.36RING-H2 finger protein ATL65 OS=Arabidopsis thaliana OX=3702 GN=ATL65 PE=2 SV=2[more]
Q9SRQ83.0e-1936.26RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Q9LY411.1e-1840.50E3 ubiquitin-protein ligase ATL4 OS=Arabidopsis thaliana OX=3702 GN=ATL4 PE=1 SV... [more]
Q9LZJ61.5e-1836.79RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1[more]
Q7XLY82.4e-1637.89E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
Match NameE-valueIdentityDescription
A0A6J1FVL76.3e-230100.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448502... [more]
A0A6J1JBG11.8e-22498.06RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111484253 P... [more]
A0A0A0LIL37.5e-20791.45RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G238760 PE... [more]
A0A1S4E2C58.6e-20391.46RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498427 PE=3 ... [more]
A0A5A7TSC82.7e-18893.21RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
XP_022943929.11.3e-229100.00RING-H2 finger protein ATL65 [Cucurbita moschata] >KAG7010952.1 RING-H2 finger p... [more]
KAG6571140.11.4e-22899.52RING-H2 finger protein ATL65, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023511773.11.1e-22598.55RING-H2 finger protein ATL65 [Cucurbita pepo subsp. pepo][more]
XP_022986546.13.7e-22498.06RING-H2 finger protein ATL65 [Cucurbita maxima][more]
XP_038902895.11.3e-20891.77RING-H2 finger protein ATL65 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G18930.14.8e-8951.36RING/U-box superfamily protein [more]
AT3G18930.24.8e-8951.36RING/U-box superfamily protein [more]
AT3G03550.12.1e-2036.26RING/U-box superfamily protein [more]
AT3G16720.12.2e-1728.46TOXICOS EN LEVADURA 2 [more]
AT5G58580.18.4e-1737.50TOXICOS EN LEVADURA 63 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 159..200
e-value: 1.5E-6
score: 37.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 158..201
e-value: 2.0E-13
score: 50.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 159..201
score: 12.208971
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 122..205
e-value: 3.8E-22
score: 79.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR45676:SF74TRANSCRIPTION FACTOR C2H2 FAMILY-RELATEDcoord: 11..384
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 158..201
e-value: 1.39094E-19
score: 79.6533
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 150..207
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 11..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G008830.1CmoCh20G008830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane