CmoCh20G008780 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G008780
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Description4-hydroxy-3-methylbut-2-enyl diphosphate reductase
LocationCmo_Chr20: 4435175 .. 4439260 (+)
RNA-Seq ExpressionCmoCh20G008780
SyntenyCmoCh20G008780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAGCAGGATCAAGATTCGGTATACCTGTTCATTGATTTCTGTATTTTAATGAGTAGAACTTTTCCGGATTTGGACTTTGAATTCGAAACGTAATTTACGAAGCTCCAACAATGCTGTGCGTTCATATTTCTACGTCAACGTCCATTCTTCGCTGTTGTTACACCACCGTTTCCCATGGCGATTTCTCTGCAGATCCCCCGTCTTCACCTACGTGTAGACTCTGTTTCATCGAAGAGATTCTCCGCCGGCGGAGCTCTCCGGTGCCGGAAACCCCTTTTGGTTCGGTGCACTGCTGATTCTTCGTCGGTCAATCCCGATTTCGATGCGAAGCTGTTTTGGAAGAACTTTTCCAGAGGTGAAAACTATAATCGGAAAGGGTTTGGGCATAAGGAGGAGACGTTGAAGCTCATGAGTAGCGAGTTCACAAGTGAGTTTTTGTTGTATTTGAATGATGATTTTAGTGTGTTGAATCCTCGTTTTGTTGGTGCCAATTTTTTTTTTTTTTTGTATTGAATGGCTTAGGTGAAGTTGTGAATACTCTGAATGAAAATGGGAATCGGTTCACTTGGGGGAATGTTACTGTCAAATTGGCTGGAGCTTATGGCTTTTGTTGGGGAGTGGAACGAGCTGTGCAGATTGCTTATGAAGCGAGGAAACAGTTTCCGAGTGAAACAATATGGATTACCAATGAGATTATTCATAACCCAACTGTTAACAAGGTCCTTTTGATGCTTTAACTTTTCTTGCCTTTACTGTTTCATTGGTTACACAATGATCACAAATGTTCTAGCACATGCTAAGGGTGTGTTTGAAATTACTTTCCGAGTGTTTTTAAGCACTTAAAATGTCATTTGAAACACTATCGTTTGGTCTGCGCTTGAAAATGTTGAGTTGTTGTTACGTTATTTTATTTAGCGACTTCAATACATAATGACATGGATACGAAAGTATGATCCATGTTGTAGTAGTACTGTAAGTATTTATGTAGTAAAGAACTTCTCCCTGTTAGGAATCATGACCCTCTACAATGGTATGATATTGTCCACTTTAAGCGTAAGCTCTCATGGTTTTGCTTTTGGTTTCCCCAAACATCCTTACTTATAAACCCATGATCTTCCTTTTATTTAGCTAATGTGGGACTACTCCTTTCAATAATCGTCAACAATCCTCCCCTCAAACAAGTTATACCATAGAGCCTCCCCTAAGGTCTATGAAGCCCTCGAACAACCTCCCCTTAATCAAGGTTCGACTTCTTTCTCTAGAGCCCTCAAACAAAGTACACCCTTCGTTCGACACTTGAATCACTTTTGACTACACCTTCGAGGCTCTCAACATCTTTGTTCGACATTTGAGGATTCTATTGACATGACTAAGTTAAGGGCATGGCTTTGATACCATATTAGAAAACACGACCCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAGGGCACGTGGTTTTGCTTTTGGTTTCCCCAAAATAACTCATAACAATGGAGATAGTGTTCCTTGGTTATAAACTCATGATTTTCCTCTTAATTAGCCAATGTAGGACTACTCCTTTCAATAATCCTCGATACTCCCAAGTTTAGATCGTACTCTTCCTTATTGTGTTGTTTTATTCTGATTTTCTTTTAGGGTTGCCAACAACATGATGTGTTGAATGAAATATCTTACTGTAACTGTTTGGAAATTCTATGAGTCTGCAGCGTTTGGAGGAGATGGGAGTGTACAACATTCCTCTGGAGGAAGGAAAGAAACGATTTGATGTCGTTAACCCTGGAGATGTTGTGATTTTGCCTGCCTTTGGGGCTGCAGTTGATGAAATGCTTGTTCTGAGTGACAAAAAAGTTAAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGTACTCCATCATTTTCTTGTCATTCACAGTCCGTCCGAGTACCATCATTGAAGGGAAGTAAGGGCACAGTGTAGCTGTTGATGCTGAGATTACCTCTGCAATTCTTTTGTATAGGTTTGGAATGCTGTTGACAAGCACAAAAAAGGAGAATACACATCAATAATTCACGGCAAGTATCGTCATGAAGAAACCGTAGCAACTGCTTCGTTCGCTGGAAAGTACATCATTGTGAAAGACATGGTAGAGGTAAATCCTTGGTACTTTTGGCTCATAGCATAAGGAAGCATTTTAAAACTAAAATATGAACCGATGTCAGGCGACGTATGTATGCGACTACATTCTTGGGGTTGAAAGTAATGGATTAATCTCAACTAAAGAAGCTTTTTTAAAGGTACAACATGTTAATGATTTGCTTAATGGAACTTGCTGATACTTTACGATATCGTCGCTCATGATAATAGTCGAGTTTCTGGCTTGTTTGTAGAAATTTAAGTCTGCTCTTTCAAAAGGATTCGACCCAGATAGTGATCTCATGAAAGTTGGCATTGCTAATCAGACCACAATGCTCAAAGGGGAAACAGAAGAGATCGGTAAATTTTTGGAGCCATTGATTTGTTAGAAACTCGCAATCTGTGAATCATTGATTTTATGATTCTGATAACAGGAAAATTGATCGAGAAAACTATGATGCGGAAGTATGGAATTGAAAATATTAATGATCACTTCATTAGTTTCAATACGATCTGTAATGCTACTCAAGTAAGTTCCACATGTTAAAGGGGTCGTGTAAAGTGTTGATATTGTTTGAAACGGATGAGATGGCAAGTTTACTGCTAGTAAGGACCTCGTGTCCGTTGATAATAGGCCGAACTTTCTCGTAATTTACAAAATTATGTATTTACTCGCGATAAAATCTCGTGATGTTGATTGGAGGTGGTGTTTTTCCTTAGGTGCGTCAAGATGCCATGTACCAGCTGGTGGAGGAAGATATGGATCTCATGTTAGTTGTTGGAGGATGGAACTCAAGCAACACCTCTCACCTTCAAGAGATTGCAGAGGCTCGAGGAATCCCGTCGTACTGGATCGACAGCGAGCAACGTATAGGGCCAGGAAACTCGATATCATACAAGCTCAATGTAAGTATGAATCTAGAATGTTGATCCAAAGACTACTTAGCATTTGTTTTTCTTTTATTGTGAGATCCCACGTTGATTGGAGTGGGGAAACAAAACATTCTTTTTAAGGGTATGGAAACCTCTCCGTAGAAGATGCGTTTTAAAATCTTGAGAGGAAGCCCAGAAAGGAAAACCCAAAAATGATAGTATCTACTAGCGGTGGGCTTGGGCCATTGCAAATAGTATCAGAGTTAAACACTGGACGATGTTTCAGTAAGCAGGTTGAACCCCGAAGGGGGTGGACACCAAGCGGTGTATCAGCGAGGACGCTGGGTCCCGAAAGAGGGTGGATTGTAAGATCCCACATCGGTTGGAGAGGGGAACAATACATTCTTTATAAGGGTGCGGAAACCTCTCCCCAACAGACGCATTTTAAGTCCTTGAGGGGAAGCCCGGAAGGGAAAGCCCAAAAAGGACAATTTCTGCTAGCTGTGGGCTTGGATTGTCACATTTATACTCATGTCAAACTCGTTGAATCGCACGACAGCACGGGGAGCTGGCGAAGAAAGAAAACTGGCTACCTGATGGACATGTTACCATTGGAGTGACCTCCGGTGCCTCTACACCAGATAAGGTAAGTTAAAAGTTGCTGTTAAACCTTGTCTGTGACTGTAAATTGTCGATCCGCCATTAACTTTTGTGAAATGGGTTAAAAATTATAGGTTGTTGGAAATGTTCTTAAGAAGGTTTTCGAGATGAAAGGGGAAGGAACTGCGCAGGTTGAATAGCTTGGATAAAAGCAAACTACAGAGTATAGCGTTACTGAACTCTTTTCTCTTCCTTTCTTTTCCATTTTTGTTTGCTAGATTATGTTTATGATATTAAAAATAGTGACATAATAAATAAACTTCACACATTTATCTTATAAAATAATCCTTGCAAATGCTTGTCAAAGGAATATCTTGAAAAGTCGAGAACTAGAGATGTCCATAAGGTGGAGCGGGGATGGAGAAACCTTCTTGTCCCTATCTTCTATTTCAATTGTCATCATGTAAAACATCGTATTAAATCCAATAA

mRNA sequence

TAAAGCAGGATCAAGATTCGGTATACCTGTTCATTGATTTCTGTATTTTAATGAGTAGAACTTTTCCGGATTTGGACTTTGAATTCGAAACGTAATTTACGAAGCTCCAACAATGCTGTGCGTTCATATTTCTACGTCAACGTCCATTCTTCGCTGTTGTTACACCACCGTTTCCCATGGCGATTTCTCTGCAGATCCCCCGTCTTCACCTACGTGTAGACTCTGTTTCATCGAAGAGATTCTCCGCCGGCGGAGCTCTCCGGTGCCGGAAACCCCTTTTGGTTCGGTGCACTGCTGATTCTTCGTCGGTCAATCCCGATTTCGATGCGAAGCTGTTTTGGAAGAACTTTTCCAGAGGTGAAAACTATAATCGGAAAGGGTTTGGGCATAAGGAGGAGACGTTGAAGCTCATGAGTAGCGAGTTCACAAGTGAAGTTGTGAATACTCTGAATGAAAATGGGAATCGGTTCACTTGGGGGAATGTTACTGTCAAATTGGCTGGAGCTTATGGCTTTTGTTGGGGAGTGGAACGAGCTGTGCAGATTGCTTATGAAGCGAGGAAACAGTTTCCGAGTGAAACAATATGGATTACCAATGAGATTATTCATAACCCAACTGTTAACAAGCGTTTGGAGGAGATGGGAGTGTACAACATTCCTCTGGAGGAAGGAAAGAAACGATTTGATGTCGTTAACCCTGGAGATGTTGTGATTTTGCCTGCCTTTGGGGCTGCAGTTGATGAAATGCTTGTTCTGAGTGACAAAAAAGTTAAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGTTTGGAATGCTGTTGACAAGCACAAAAAAGGAGAATACACATCAATAATTCACGGCAAGTATCGTCATGAAGAAACCGTAGCAACTGCTTCGTTCGCTGGAAAGTACATCATTGTGAAAGACATGGTAGAGGCGACGTATGTATGCGACTACATTCTTGGGGTTGAAAGTAATGGATTAATCTCAACTAAAGAAGCTTTTTTAAAGAAATTTAAGTCTGCTCTTTCAAAAGGATTCGACCCAGATAGTGATCTCATGAAAGTTGGCATTGCTAATCAGACCACAATGCTCAAAGGGGAAACAGAAGAGATCGGAAAATTGATCGAGAAAACTATGATGCGGAAGTATGGAATTGAAAATATTAATGATCACTTCATTAGTTTCAATACGATCTGTAATGCTACTCAAGTGCGTCAAGATGCCATGTACCAGCTGGTGGAGGAAGATATGGATCTCATGTTAGTTGTTGGAGGATGGAACTCAAGCAACACCTCTCACCTTCAAGAGATTGCAGAGGCTCGAGGAATCCCGTCGTACTGGATCGACAGCGAGCAACGTATAGGGCCAGGAAACTCGATATCATACAAGCTCAATCACGGGGAGCTGGCGAAGAAAGAAAACTGGCTACCTGATGGACATGTTACCATTGGAGTGACCTCCGGTGCCTCTACACCAGATAAGGTTGTTGGAAATGTTCTTAAGAAGGTTTTCGAGATGAAAGGGGAAGGAACTGCGCAGGTTGAATAGCTTGGATAAAAGCAAACTACAGAGTATAGCGTTACTGAACTCTTTTCTCTTCCTTTCTTTTCCATTTTTGTTTGCTAGATTATGTTTATGATATTAAAAATAGTGACATAATAAATAAACTTCACACATTTATCTTATAAAATAATCCTTGCAAATGCTTGTCAAAGGAATATCTTGAAAAGTCGAGAACTAGAGATGTCCATAAGGTGGAGCGGGGATGGAGAAACCTTCTTGTCCCTATCTTCTATTTCAATTGTCATCATGTAAAACATCGTATTAAATCCAATAA

Coding sequence (CDS)

ATGGCGATTTCTCTGCAGATCCCCCGTCTTCACCTACGTGTAGACTCTGTTTCATCGAAGAGATTCTCCGCCGGCGGAGCTCTCCGGTGCCGGAAACCCCTTTTGGTTCGGTGCACTGCTGATTCTTCGTCGGTCAATCCCGATTTCGATGCGAAGCTGTTTTGGAAGAACTTTTCCAGAGGTGAAAACTATAATCGGAAAGGGTTTGGGCATAAGGAGGAGACGTTGAAGCTCATGAGTAGCGAGTTCACAAGTGAAGTTGTGAATACTCTGAATGAAAATGGGAATCGGTTCACTTGGGGGAATGTTACTGTCAAATTGGCTGGAGCTTATGGCTTTTGTTGGGGAGTGGAACGAGCTGTGCAGATTGCTTATGAAGCGAGGAAACAGTTTCCGAGTGAAACAATATGGATTACCAATGAGATTATTCATAACCCAACTGTTAACAAGCGTTTGGAGGAGATGGGAGTGTACAACATTCCTCTGGAGGAAGGAAAGAAACGATTTGATGTCGTTAACCCTGGAGATGTTGTGATTTTGCCTGCCTTTGGGGCTGCAGTTGATGAAATGCTTGTTCTGAGTGACAAAAAAGTTAAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGTTTGGAATGCTGTTGACAAGCACAAAAAAGGAGAATACACATCAATAATTCACGGCAAGTATCGTCATGAAGAAACCGTAGCAACTGCTTCGTTCGCTGGAAAGTACATCATTGTGAAAGACATGGTAGAGGCGACGTATGTATGCGACTACATTCTTGGGGTTGAAAGTAATGGATTAATCTCAACTAAAGAAGCTTTTTTAAAGAAATTTAAGTCTGCTCTTTCAAAAGGATTCGACCCAGATAGTGATCTCATGAAAGTTGGCATTGCTAATCAGACCACAATGCTCAAAGGGGAAACAGAAGAGATCGGAAAATTGATCGAGAAAACTATGATGCGGAAGTATGGAATTGAAAATATTAATGATCACTTCATTAGTTTCAATACGATCTGTAATGCTACTCAAGTGCGTCAAGATGCCATGTACCAGCTGGTGGAGGAAGATATGGATCTCATGTTAGTTGTTGGAGGATGGAACTCAAGCAACACCTCTCACCTTCAAGAGATTGCAGAGGCTCGAGGAATCCCGTCGTACTGGATCGACAGCGAGCAACGTATAGGGCCAGGAAACTCGATATCATACAAGCTCAATCACGGGGAGCTGGCGAAGAAAGAAAACTGGCTACCTGATGGACATGTTACCATTGGAGTGACCTCCGGTGCCTCTACACCAGATAAGGTTGTTGGAAATGTTCTTAAGAAGGTTTTCGAGATGAAAGGGGAAGGAACTGCGCAGGTTGAATAG

Protein sequence

MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE
Homology
BLAST of CmoCh20G008780 vs. ExPASy Swiss-Prot
Match: Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 9.2e-195
Identity = 334/465 (71.83%), Postives = 395/465 (84.95%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDS-VSSKRFSAGGALRCRKPLLVRCTA-DSSSVNP------DFDAK 60
           MA++LQ  RL +R D+ V     S  G+LR RK L VRC++ D ++ +P      DFDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  LFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYG 120
           +F KN +R +NYNRKGFGHKEETLKLM+ E+TS+++ TL  NG  ++WG+VTVKLA AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVV 180
           FCWGVERAVQIAYEARKQFP E +WITNEIIHNPTVNKRLE+M V  IP+E+ KK+FDVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 NPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYR 240
              DVVILPAFGA VDEM VL+DKKV+IVDTTCPWV+KVWN V+KHKKGEYTS+IHGKY 
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYN 240

Query: 241 HEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPD 300
           HEET+ATASFAGKYIIVK+M EA YVCDYILG + +G  STKE F++KFK A+SKGFDPD
Sbjct: 241 HEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPD 300

Query: 301 SDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAM 360
           +DL+KVGIANQTTMLKGETEEIG+L+E TMMRKYG+EN++ HFISFNTIC+ATQ RQDA+
Sbjct: 301 NDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDAI 360

Query: 361 YQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGEL 420
           Y+LVEE +DLMLVVGGWNSSNTSHLQEI+EARGIPSYWIDSE+RIGPGN I+YKL++GEL
Sbjct: 361 YELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGEL 420

Query: 421 AKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQV 458
            +KEN+LP G +TIGVTSGASTPDKVV + L KVF++K E   Q+
Sbjct: 421 VEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQL 465

BLAST of CmoCh20G008780 vs. ExPASy Swiss-Prot
Match: Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 7.3e-192
Identity = 323/433 (74.60%), Postives = 373/433 (86.14%), Query Frame = 0

Query: 31  RKPLLVRCTAD-------SSSVNPDFDAKLFWKNFSRGENYNRKGFGHKEETLKLMSSEF 90
           R P  VRC +        S+S++ DFD K F  N +R +NYNRKGFGHK+ETL+LMS E+
Sbjct: 25  RAPSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSDNYNRKGFGHKKETLELMSQEY 84

Query: 91  TSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWITNEII 150
           TS+V+ TL ENGN+ TWG VTVKLA AYGFCWGVERAVQIAYEARKQFP + IW+TNEII
Sbjct: 85  TSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQIAYEARKQFPDDRIWLTNEII 144

Query: 151 HNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLSDKKVKIVDT 210
           HNPTVNKRLE+MGV NIP++ G K FDVV  GDVV+LPAFGAAV+EM  L++KKV+IVDT
Sbjct: 145 HNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAFGAAVEEMYTLNEKKVQIVDT 204

Query: 211 TCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYVCDYIL 270
           TCPWVSKVWN V+KHKKG+YTSIIHGKY HEETVATASFAG YIIVK++ EA+YVCDYIL
Sbjct: 205 TCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEETVATASFAGTYIIVKNIAEASYVCDYIL 264

Query: 271 GVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMM 330
           G + +G  STKE FL+KFK+A+S GFDPD DL+KVGIANQTTMLKGETEEIGKL+EKTMM
Sbjct: 265 GGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQTTMLKGETEEIGKLVEKTMM 324

Query: 331 RKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEA 390
           R++G+EN+NDHFI+FNTIC+ATQ RQDAMYQLV+E +DL+LVVGGWNSSNTSHLQEI E 
Sbjct: 325 RRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLILVVGGWNSSNTSHLQEIGEL 384

Query: 391 RGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKVVGNVL 450
            GIPSYWIDSEQRIGPGN ISYKLNHGEL +KENWLP+G +TIGVTSGASTPDKVV + L
Sbjct: 385 SGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGPITIGVTSGASTPDKVVEDAL 444

Query: 451 KKVFEMKGEGTAQ 457
           +KVFE+K +   Q
Sbjct: 445 QKVFEIKRQEVLQ 457

BLAST of CmoCh20G008780 vs. ExPASy Swiss-Prot
Match: B8HWD3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=ispH PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 2.3e-145
Identity = 252/409 (61.61%), Postives = 316/409 (77.26%), Query Frame = 0

Query: 50  DAKLFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAG 109
           D K F ++      Y+RKGFGH+ E  +++  E+ S ++  + ENGNRF  G VT++LA 
Sbjct: 2   DTKAFKRSLHSSNQYHRKGFGHEAEVTEMLRFEYQSSLIQQIRENGNRFQRGEVTIQLAE 61

Query: 110 AYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPL-EEGKKR 169
           A+GFCWGVERAV +AYE R+ FP+E IWITNEIIHNP+VN+ L EM V  IP+ E+G+K 
Sbjct: 62  AFGFCWGVERAVALAYETRQHFPTERIWITNEIIHNPSVNQNLREMQVEFIPVDEQGQKD 121

Query: 170 FDVVNPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIH 229
           F VV   DVVILPAFGA+V EM +L+DK  KI+DTTCPWVSKVWN+V+KHKKG YTSIIH
Sbjct: 122 FSVVAGEDVVILPAFGASVQEMQLLNDKGCKIMDTTCPWVSKVWNSVEKHKKGSYTSIIH 181

Query: 230 GKYRHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKG 289
           GKY+HEET+AT+S+A  Y+IV D+ EA YVC+YIL    NG    +E FL KF+ A S  
Sbjct: 182 GKYQHEETIATSSYAATYLIVLDLKEAEYVCNYIL----NG--GDREEFLHKFRHAYSPD 241

Query: 290 FDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVR 349
           FDPD DL++VGIANQTTMLKGETE+IGKL E+T+MRKYG + IN HF+SFNTIC+ATQ R
Sbjct: 242 FDPDRDLVRVGIANQTTMLKGETEQIGKLFERTLMRKYGPDQINQHFLSFNTICDATQER 301

Query: 350 QDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN 409
           QDAM++LVE  +DLM+V+GG+NSSNT+HLQEI+  R IPSY IDS  RIGPGN + +K  
Sbjct: 302 QDAMFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNRVEHKPL 361

Query: 410 HGELAKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQV 458
           H  L  KENWLP+G + +G+TSGASTPDKVV +VL+K+F +K    A +
Sbjct: 362 HRPLEIKENWLPEGPIVVGITSGASTPDKVVSDVLEKIFALKSTSPALI 404

BLAST of CmoCh20G008780 vs. ExPASy Swiss-Prot
Match: B1WTZ2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=ispH PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 9.6e-144
Identity = 248/404 (61.39%), Postives = 316/404 (78.22%), Query Frame = 0

Query: 50  DAKLFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAG 109
           D K F ++  +  NY+RKGFGH+ E +  M++E+ S ++  + EN  ++  G+VT+KLA 
Sbjct: 2   DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61

Query: 110 AYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRF 169
           A+GFCWGVERAV +AYE R+ FP E IWITNEIIHNP+VN+RL EM V  I +  G K F
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRLLEMNVGFIEVINGSKDF 121

Query: 170 DVVNPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHG 229
            VV  GDVVILPAFGA+V EM +L+DK   IVDTTCPWVSKVWN+V+KHKK +YTSIIHG
Sbjct: 122 SVVEAGDVVILPAFGASVTEMQLLNDKGCTIVDTTCPWVSKVWNSVEKHKKKDYTSIIHG 181

Query: 230 KYRHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGF 289
           KY+HEETVAT+SFAG Y++V ++ EA YVCDYIL    NG    K+ FL+KFK+A S+GF
Sbjct: 182 KYKHEETVATSSFAGTYLVVLNLKEAQYVCDYIL----NG--GNKDEFLEKFKNAHSEGF 241

Query: 290 DPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQ 349
           DPD DL+++GIANQTTMLK ETE+IGKL E TM++KYG   +N+HF+SFNTIC+ATQ RQ
Sbjct: 242 DPDKDLIRLGIANQTTMLKSETEQIGKLFETTMLKKYGPTELNEHFMSFNTICDATQERQ 301

Query: 350 DAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNH 409
           DAM  LVEE++D+M+V+GG+NSSNT+HLQEI+  +GI SY IDS++RI PGN I +K   
Sbjct: 302 DAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDSDKRILPGNKIEHKPLE 361

Query: 410 GELAKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEG 454
            ++  KENWLPDG + +GVTSGASTPDKVV  V++++FE K  G
Sbjct: 362 KDIETKENWLPDGKIIVGVTSGASTPDKVVEAVIERIFEEKNAG 399

BLAST of CmoCh20G008780 vs. ExPASy Swiss-Prot
Match: Q8DK29 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=ispH PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.2e-141
Identity = 246/405 (60.74%), Postives = 307/405 (75.80%), Query Frame = 0

Query: 50  DAKLFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAG 109
           D + F ++    ENY+RKGFGH EE  + +  E+ S ++  +  NG R+  G+VT++LA 
Sbjct: 2   DTRAFKRSLHSSENYHRKGFGHGEEVNQQLQGEYQSSLIQQIRANGYRWQQGDVTIRLAE 61

Query: 110 AYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRF 169
           A+GFCWGVERAV +AYE R  FP+E IWITNEIIHNP+VN+RL +M V  IP+  G K F
Sbjct: 62  AFGFCWGVERAVALAYETRTHFPTERIWITNEIIHNPSVNERLRQMAVEFIPVVNGVKDF 121

Query: 170 DVVNPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHG 229
             V PGDVVILPAFGA+V EM +L+++   IVDTTCPWVSKVW++V+KHKK  +TSIIHG
Sbjct: 122 SGVRPGDVVILPAFGASVQEMQLLNERGCTIVDTTCPWVSKVWHSVEKHKKVSFTSIIHG 181

Query: 230 KYRHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGF 289
           KY HEET+AT+SFAG Y+IV ++ EA YVCDYIL    +G    + AF+ KF  A S GF
Sbjct: 182 KYNHEETIATSSFAGTYLIVLNLEEARYVCDYIL----HG--GDRAAFMAKFAKACSPGF 241

Query: 290 DPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQ 349
           DPD DL++VGIANQTTMLKGETE+IGKL E+TM++KYG + +N+HF+SFNTIC+ATQ RQ
Sbjct: 242 DPDRDLVRVGIANQTTMLKGETEQIGKLFERTMIQKYGPDRLNEHFMSFNTICDATQERQ 301

Query: 350 DAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNH 409
           DAM  LV+E +DLM+V+GG+NSSNT+HLQEIA   GIPSY IDS  RIGPGN I +K  H
Sbjct: 302 DAMLSLVKEPLDLMVVIGGYNSSNTTHLQEIAIEHGIPSYHIDSADRIGPGNRIEHKPLH 361

Query: 410 GELAKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGT 455
                 ENWLPD  +TIG+TSGASTPDKVV  VL K+F ++   T
Sbjct: 362 QNPTVAENWLPDRPITIGITSGASTPDKVVEEVLNKIFALRSVAT 400

BLAST of CmoCh20G008780 vs. ExPASy TrEMBL
Match: A0A6J1FWL7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448851 PE=3 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 7.2e-267
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300
           SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 459
           DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 458

BLAST of CmoCh20G008780 vs. ExPASy TrEMBL
Match: A0A6J1JG99 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484238 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.2e-255
Identity = 439/458 (95.85%), Postives = 447/458 (97.60%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRL LRVDSVSSKRFS GGALRC KPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLPLRVDSVSSKRFSGGGALRCWKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGF HKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFEHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVLSDKKV IVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKY HEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVTIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYHHEETVATA 240

Query: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300
           SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKE FLKKFKSA+SKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEDFLKKFKSAVSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLK ET+EIGKLIEKTMMRKYGIENI++HFISFNTICNATQVRQDAMYQLVEED 
Sbjct: 301 ANQTTMLKEETKEIGKLIEKTMMRKYGIENISEHFISFNTICNATQVRQDAMYQLVEEDT 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGEL KKENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLKHGELVKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 459
           DGH+TIGVTSGASTPD+VVGNVLKKVFE+KGEGTAQ+E
Sbjct: 421 DGHITIGVTSGASTPDQVVGNVLKKVFEIKGEGTAQLE 458

BLAST of CmoCh20G008780 vs. ExPASy TrEMBL
Match: A0A1S3CAB0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498435 PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 8.3e-231
Identity = 398/463 (85.96%), Postives = 428/463 (92.44%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSS----VNPDFDAKLFWK 60
           MAISLQ PR   R+D    K FS  GALRCR+PLL+RCTADSSS    V+PDF+AKLF K
Sbjct: 1   MAISLQSPRFPHRIDFSLPKTFSGVGALRCRQPLLLRCTADSSSSPKPVDPDFNAKLFLK 60

Query: 61  NFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWG 120
           NFSRGENYNRKGFGHKEET+KLM+SEFTSE+V TL ENG +FTWGNVTVKLAGAYGFCWG
Sbjct: 61  NFSRGENYNRKGFGHKEETVKLMNSEFTSEIVTTLKENGFQFTWGNVTVKLAGAYGFCWG 120

Query: 121 VERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEG-KKRFDVVNPG 180
           VERAVQ AYEARKQ+PSETIWITNEIIHNPTVNKRLEEMGV+NIPLEEG KKRFD VNPG
Sbjct: 121 VERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRLEEMGVHNIPLEEGNKKRFDAVNPG 180

Query: 181 DVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEE 240
           DVVILPAFGAAVDE+LVL+DKKVKIVDTTCPWVSKVWNAV KHKKGEYTSIIHGKY HEE
Sbjct: 181 DVVILPAFGAAVDEILVLNDKKVKIVDTTCPWVSKVWNAVGKHKKGEYTSIIHGKYSHEE 240

Query: 241 TVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDL 300
           T+ATASFAGKYIIVKDM EA YV DYILGVE +G ISTKE FLKKFKSA+SKGFDPD  L
Sbjct: 241 TIATASFAGKYIIVKDMAEAAYVSDYILGVEHDGSISTKEEFLKKFKSAVSKGFDPDIHL 300

Query: 301 MKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQL 360
           +K+GIANQTTMLKGETEEIGKL+EKTMMRKYG+ENIN+HFISFNTICNATQVRQDAMY+L
Sbjct: 301 VKIGIANQTTMLKGETEEIGKLMEKTMMRKYGVENINEHFISFNTICNATQVRQDAMYEL 360

Query: 361 VEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKK 420
           V+ED+DLMLVVGGWNSSNTSHLQEIAEARG+PSYWIDSEQRIGPGNSISYKL HGELA K
Sbjct: 361 VKEDVDLMLVVGGWNSSNTSHLQEIAEARGVPSYWIDSEQRIGPGNSISYKLAHGELANK 420

Query: 421 ENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 459
           ENWLPDGH+TIGVTSGASTPDKVVG+VLKKVF++KG+GTAQ E
Sbjct: 421 ENWLPDGHITIGVTSGASTPDKVVGDVLKKVFDIKGDGTAQFE 463

BLAST of CmoCh20G008780 vs. ExPASy TrEMBL
Match: A0A6J1D5C2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111017170 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.3e-223
Identity = 390/464 (84.05%), Postives = 423/464 (91.16%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDSVSSKRFSAGGA--LRCRKPLLVRCTADSSS----VNPDFDAKLF 60
           MAISLQIPR+  R   +S    SA GA  LR RKP  +RCTADS S    ++PDFDAKLF
Sbjct: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60

Query: 61  WKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120
           WKN++RGENYNRKGFGHKEET+KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120

Query: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180

Query: 181 GDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240
           GDVVILPAFGAAVDEMLVL DK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLKDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240

Query: 241 ETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSD 300
           ET+ATASFAGKYIIVK+M EATYV D+ILGVE NG I TKE FLKKF+SA+S+GFDPD D
Sbjct: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300

Query: 301 LMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQV-RQDAMY 360
           L+KVGIANQTTMLKGETEEIGKLIE+TMMRKYG+EN+N+HF+SFNTIC+ATQV RQDAMY
Sbjct: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQVKRQDAMY 360

Query: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELA 420
           QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL +GELA
Sbjct: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIYGELA 420

Query: 421 KKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQV 458
           +KENWLPDGHVTIGVTSGASTPDKVV NVLKKVF++KGEGT Q+
Sbjct: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464

BLAST of CmoCh20G008780 vs. ExPASy TrEMBL
Match: A0A6J1FYM6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448851 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.1e-219
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 79  MSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWI 138
           MSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWI
Sbjct: 1   MSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWI 60

Query: 139 TNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLSDKKV 198
           TNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLSDKKV
Sbjct: 61  TNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLSDKKV 120

Query: 199 KIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYV 258
           KIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYV
Sbjct: 121 KIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYV 180

Query: 259 CDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLI 318
           CDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLI
Sbjct: 181 CDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLI 240

Query: 319 EKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQ 378
           EKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQ
Sbjct: 241 EKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQ 300

Query: 379 EIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKV 438
           EIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKV
Sbjct: 301 EIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKV 360

Query: 439 VGNVLKKVFEMKGEGTAQVE 459
           VGNVLKKVFEMKGEGTAQVE
Sbjct: 361 VGNVLKKVFEMKGEGTAQVE 380

BLAST of CmoCh20G008780 vs. NCBI nr
Match: XP_022944389.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 929.1 bits (2400), Expect = 1.5e-266
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300
           SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 459
           DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 458

BLAST of CmoCh20G008780 vs. NCBI nr
Match: KAG7010948.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 927.5 bits (2396), Expect = 4.3e-266
Identity = 457/458 (99.78%), Postives = 457/458 (99.78%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRLHLRVDSVSSKRFS GGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSGGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300
           SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 459
           DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 458

BLAST of CmoCh20G008780 vs. NCBI nr
Match: KAG6571137.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 924.5 bits (2388), Expect = 3.6e-265
Identity = 455/458 (99.34%), Postives = 455/458 (99.34%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRLHLRVDSVSSKRFS GGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSGGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300
           SFAGKYI VKDM EATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIFVKDMAEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 459
           DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 458

BLAST of CmoCh20G008780 vs. NCBI nr
Match: XP_023512039.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 901.7 bits (2329), Expect = 2.5e-258
Identity = 445/458 (97.16%), Postives = 451/458 (98.47%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRL  RVDSVSSK FS  GALR RKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFG+KEE LKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVL+DKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300
           SFAGKYIIVKD+VEATYVCDYILGVESNGLISTKEAFLKKFKSA+SKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 459
           DGHVTIGVTSGASTPDKVVGNVLKKVFE+KGEGTAQ+E
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 458

BLAST of CmoCh20G008780 vs. NCBI nr
Match: XP_022986524.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 891.0 bits (2301), Expect = 4.5e-255
Identity = 439/458 (95.85%), Postives = 447/458 (97.60%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRL LRVDSVSSKRFS GGALRC KPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLPLRVDSVSSKRFSGGGALRCWKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGF HKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFEHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVLSDKKV IVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKY HEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVTIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYHHEETVATA 240

Query: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300
           SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKE FLKKFKSA+SKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEDFLKKFKSAVSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLK ET+EIGKLIEKTMMRKYGIENI++HFISFNTICNATQVRQDAMYQLVEED 
Sbjct: 301 ANQTTMLKEETKEIGKLIEKTMMRKYGIENISEHFISFNTICNATQVRQDAMYQLVEEDT 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGEL KKENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLKHGELVKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 459
           DGH+TIGVTSGASTPD+VVGNVLKKVFE+KGEGTAQ+E
Sbjct: 421 DGHITIGVTSGASTPDQVVGNVLKKVFEIKGEGTAQLE 458

BLAST of CmoCh20G008780 vs. TAIR 10
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )

HSP 1 Score: 681.0 bits (1756), Expect = 6.5e-196
Identity = 334/465 (71.83%), Postives = 395/465 (84.95%), Query Frame = 0

Query: 1   MAISLQIPRLHLRVDS-VSSKRFSAGGALRCRKPLLVRCTA-DSSSVNP------DFDAK 60
           MA++LQ  RL +R D+ V     S  G+LR RK L VRC++ D ++ +P      DFDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  LFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYG 120
           +F KN +R +NYNRKGFGHKEETLKLM+ E+TS+++ TL  NG  ++WG+VTVKLA AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVV 180
           FCWGVERAVQIAYEARKQFP E +WITNEIIHNPTVNKRLE+M V  IP+E+ KK+FDVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 NPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYR 240
              DVVILPAFGA VDEM VL+DKKV+IVDTTCPWV+KVWN V+KHKKGEYTS+IHGKY 
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYN 240

Query: 241 HEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPD 300
           HEET+ATASFAGKYIIVK+M EA YVCDYILG + +G  STKE F++KFK A+SKGFDPD
Sbjct: 241 HEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPD 300

Query: 301 SDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAM 360
           +DL+KVGIANQTTMLKGETEEIG+L+E TMMRKYG+EN++ HFISFNTIC+ATQ RQDA+
Sbjct: 301 NDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDAI 360

Query: 361 YQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGEL 420
           Y+LVEE +DLMLVVGGWNSSNTSHLQEI+EARGIPSYWIDSE+RIGPGN I+YKL++GEL
Sbjct: 361 YELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGEL 420

Query: 421 AKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQV 458
            +KEN+LP G +TIGVTSGASTPDKVV + L KVF++K E   Q+
Sbjct: 421 VEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQL 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94B359.2e-19571.834-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis... [more]
Q6AVG67.3e-19274.604-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ... [more]
B8HWD32.3e-14561.614-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC... [more]
B1WTZ29.6e-14461.394-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (s... [more]
Q8DK291.2e-14160.744-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elonga... [more]
Match NameE-valueIdentityDescription
A0A6J1FWL77.2e-267100.004-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A6J1JG992.2e-25595.854-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A1S3CAB08.3e-23185.964-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A6J1D5C21.3e-22384.054-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momord... [more]
A0A6J1FYM61.1e-219100.004-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
Match NameE-valueIdentityDescription
XP_022944389.11.5e-266100.004-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
KAG7010948.14.3e-26699.784-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucu... [more]
KAG6571137.13.6e-26599.344-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucu... [more]
XP_023512039.12.5e-25897.164-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
XP_022986524.14.5e-25595.854-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
Match NameE-valueIdentityDescription
AT4G34350.16.5e-19671.834-hydroxy-3-methylbut-2-enyl diphosphate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.1010.20coord: 326..458
e-value: 9.6E-40
score: 136.8
NoneNo IPR availableGENE3D3.40.1010.20coord: 206..325
e-value: 2.3E-11
score: 45.4
NoneNo IPR availableGENE3D3.40.50.11270coord: 114..204
e-value: 1.8E-21
score: 77.9
NoneNo IPR availablePANTHERPTHR316194-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTICcoord: 5..454
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseTIGRFAMTIGR00216TIGR00216coord: 105..447
e-value: 3.9E-90
score: 299.8
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductasePFAMPF02401LYTBcoord: 105..446
e-value: 2.0E-83
score: 279.7
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseHAMAPMF_00191IspHcoord: 103..450
score: 28.883177
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseCDDcd13944lytB_ispHcoord: 105..446
e-value: 1.19654E-106
score: 315.504

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G008780.1CmoCh20G008780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050992 dimethylallyl diphosphate biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0051745 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function GO:0046429 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding