CmoCh20G007530 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G007530
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionsubtilisin-like protease SBT1.6
LocationCmo_Chr20: 3786305 .. 3788629 (-)
RNA-Seq ExpressionCmoCh20G007530
SyntenyCmoCh20G007530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCTTATCTCTCCCCTCTCTTCTTCTTCTTCTTCTTCTTCTTCCTTTTGATTGTTCATCGATTTACAGTTTCGGCCGATTCGCCATTGAAGAAGACCTTCATCTTCCGTGTGGATCGTTTCTCTAAGCCCTCTGTTTTTCCGACCCATTACCACTGGTACACTTCTGAGTTTGCAGAATCCCACAAAATCCTCCATGTTTACGACACCGTTTTCCATGGATTCTCTGCAACTCTTACTCAACAACAAGTTGATTCCATCGGGAAACACCCTTCTGTGCTTGCCGTTTTTGAGGATCGTCGCCGGCAGCTTCACACCACCCGTTCCCCTCAGTTTCTTGGCCTTCGAAACCAGCGTGGTCTCTGGTCGGATTCTGATTATGGGTCGGATGTAATCATTGGGGTTTTCGACACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGGCGGTGGAAGGGGGTTTGTGAGACGGGAACTAAGTTCACGGCTAGAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCATGAAGCTGGGTCCAACGCTGCTGGTCCGATTATTGGGATTAACGATACGATTGAGTTTAGATCTCCGAGAGATGCCGACGGCCATGGGACTCATACGGCCTCCACGGCGGCTGGACGGCATGCGTTTCAGGCGAGTTTGGAGGGTTTTGCTTCTGGGATAGCGAAGGGTGTGGCTCCTAAAGCGCGTTTGGCGGTTTATAAAGTTTGTTGGAAAAATTCTGGTTGTTTTGATTCTGACATTCTTGCGGCGTTTGATGCGGCGGTTAACGACGGCGTGGATGTCATTTCGATCTCGATTGGAGGCGGCGATGGGGTTTCCTCGCCGTATTATCTCGACCCAATTGCAATCGGAGCGTACGGCGCCGCTTCCAGGGGTATCTTCGTTTCGTCTTCGGCTGGGAATGATGGACCTAATGGAATGTCAGTGACGAACTTGGCGCCGTGGGTTACGACCGTTGGAGCCGGTACGATTGACCGTAATTTCCCGGCGGTGGTGACTCTGGGTAACGGACGGAGGATTTCCGGCGTGTCACTCTACGCCGGAGCGCCTTTGAACGGTACAATGTTTCCATTGGTTTACCCTGGGAAATCAGGGGTGCTCTCTGTTTCGCTCTGTATGGATAACTCTCTCGATCCTAAGGTTGTTGCCGGAAAAATTGTGATCTGCGACAGGGGAAGTAGTCCCAGAGTGGCTAAGGGTTTGGTCGTTAAGAAAGCTGGCGGCGTCGGAATGATTCTGGCGAATGGAATCTCAAACGGCGAAGGACTCGTCGGCGATGCCCATCTTCTCCCCGCCTGCGCAGTCGGCTCCGACGAAGGCGATTCCATGAAAGCCTACGCATCGTCCTCTCCAAATCCCACAGCTACCATCGCCTTCCAGGGCACTATAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCCTTCTCAGCAAGAGGGCCAAATGGATTAAACCCAGAAATTCTAAAACCAGACATAATCGCACCAGGGGTTAACATTCTCGCCGCCTGGACCGACGCTGTCGGTCCAACCGGTTTGGACTTTGATACAAGAAAAACAGAGTTCAACATCTTGTCTGGTACATCAATGGCGTGTCCTCATGTAAGTGGTGCCGCTGCTCTGTTGAAATCAGCTCACCCAGATTGGAGTCCAGCGGCGTTAAGATCCGCCATGATGACCACCGCAAGTACCACCGACAACCGACGACAGCCGATGACGGAAGAATCCACCGGAAAAGCTTCAACTCCGTACGATTTCGGTGCAGGGCATGTAAATTTGGGTCTAGCAATGGACCCTGGTTTAGTCTACGACATTACAAACACGGATTACATCAACTTCTTGTGCTCAATCGGATACGGACCGAAGATGATTCAAGTGATAACAAGAACGCCGGTAACCTGTCCGGCGAAAAAACCATTGCCGGAAAATCTCAATTACCCATCAATCGTTGCAGTATTCTCCAGTCTATCAAAAGGGTGGTCGACAAAATCGTTTATCCGAACAGTGACGAACGTGGGTCCTGCCAATTCGGTGTACCGTGCCAAAATCGAAGCTCCAAAAGGGGTAACGGTGAAGGTAAAGCCTTCGAAGCTCGTGTTCTCAGCGGCGACGAAGAAACAGAGCTTCGTTGTAGCAGTGTCAGCCGACAACCAGAATCTAGCACTGGGCGACGTGGGCGCTGTTTTTGGTTGGGTTTCTTGGTCGGATGGAAAGCATGTGGTCCGAAGCCCACTCGTAGTAACCCAATTAGAGCCGTTGTAA

mRNA sequence

ATGGCCGCTTATCTCTCCCCTCTCTTCTTCTTCTTCTTCTTCTTCTTCCTTTTGATTGTTCATCGATTTACAGTTTCGGCCGATTCGCCATTGAAGAAGACCTTCATCTTCCGTGTGGATCGTTTCTCTAAGCCCTCTGTTTTTCCGACCCATTACCACTGGTACACTTCTGAGTTTGCAGAATCCCACAAAATCCTCCATGTTTACGACACCGTTTTCCATGGATTCTCTGCAACTCTTACTCAACAACAAGTTGATTCCATCGGGAAACACCCTTCTGTGCTTGCCGTTTTTGAGGATCGTCGCCGGCAGCTTCACACCACCCGTTCCCCTCAGTTTCTTGGCCTTCGAAACCAGCGTGGTCTCTGGTCGGATTCTGATTATGGGTCGGATGTAATCATTGGGGTTTTCGACACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGGCGGTGGAAGGGGGTTTGTGAGACGGGAACTAAGTTCACGGCTAGAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCATGAAGCTGGGTCCAACGCTGCTGGTCCGATTATTGGGATTAACGATACGATTGAGTTTAGATCTCCGAGAGATGCCGACGGCCATGGGACTCATACGGCCTCCACGGCGGCTGGACGGCATGCGTTTCAGGCGAGTTTGGAGGGTTTTGCTTCTGGGATAGCGAAGGGTGTGGCTCCTAAAGCGCGTTTGGCGGTTTATAAAGTTTGTTGGAAAAATTCTGGTTGTTTTGATTCTGACATTCTTGCGGCGTTTGATGCGGCGGTTAACGACGGCGTGGATGTCATTTCGATCTCGATTGGAGGCGGCGATGGGGTTTCCTCGCCGTATTATCTCGACCCAATTGCAATCGGAGCGTACGGCGCCGCTTCCAGGGGTATCTTCGTTTCGTCTTCGGCTGGGAATGATGGACCTAATGGAATGTCAGTGACGAACTTGGCGCCGTGGGTTACGACCGTTGGAGCCGGTACGATTGACCGTAATTTCCCGGCGGTGGTGACTCTGGGTAACGGACGGAGGATTTCCGGCGTGTCACTCTACGCCGGAGCGCCTTTGAACGGTACAATGTTTCCATTGGTTTACCCTGGGAAATCAGGGGTGCTCTCTGTTTCGCTCTGTATGGATAACTCTCTCGATCCTAAGGTTGTTGCCGGAAAAATTGTGATCTGCGACAGGGGAAGTAGTCCCAGAGTGGCTAAGGGTTTGGTCGTTAAGAAAGCTGGCGGCGTCGGAATGATTCTGGCGAATGGAATCTCAAACGGCGAAGGACTCGTCGGCGATGCCCATCTTCTCCCCGCCTGCGCAGTCGGCTCCGACGAAGGCGATTCCATGAAAGCCTACGCATCGTCCTCTCCAAATCCCACAGCTACCATCGCCTTCCAGGGCACTATAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCCTTCTCAGCAAGAGGGCCAAATGGATTAAACCCAGAAATTCTAAAACCAGACATAATCGCACCAGGGGTTAACATTCTCGCCGCCTGGACCGACGCTGTCGGTCCAACCGGTTTGGACTTTGATACAAGAAAAACAGAGTTCAACATCTTGTCTGGTACATCAATGGCGTGTCCTCATGTAAGTGGTGCCGCTGCTCTGTTGAAATCAGCTCACCCAGATTGGAGTCCAGCGGCGTTAAGATCCGCCATGATGACCACCGCAAGTACCACCGACAACCGACGACAGCCGATGACGGAAGAATCCACCGGAAAAGCTTCAACTCCGTACGATTTCGGTGCAGGGCATGTAAATTTGGGTCTAGCAATGGACCCTGGTTTAGTCTACGACATTACAAACACGGATTACATCAACTTCTTGTGCTCAATCGGATACGGACCGAAGATGATTCAAGTGATAACAAGAACGCCGGTAACCTGTCCGGCGAAAAAACCATTGCCGGAAAATCTCAATTACCCATCAATCGTTGCAGTATTCTCCAGTCTATCAAAAGGGTGGTCGACAAAATCGTTTATCCGAACAGTGACGAACGTGGGTCCTGCCAATTCGGTGTACCGTGCCAAAATCGAAGCTCCAAAAGGGGTAACGGTGAAGGTAAAGCCTTCGAAGCTCGTGTTCTCAGCGGCGACGAAGAAACAGAGCTTCGTTGTAGCAGTGTCAGCCGACAACCAGAATCTAGCACTGGGCGACGTGGGCGCTGTTTTTGGTTGGGTTTCTTGGTCGGATGGAAAGCATGTGGTCCGAAGCCCACTCGTAGTAACCCAATTAGAGCCGTTGTAA

Coding sequence (CDS)

ATGGCCGCTTATCTCTCCCCTCTCTTCTTCTTCTTCTTCTTCTTCTTCCTTTTGATTGTTCATCGATTTACAGTTTCGGCCGATTCGCCATTGAAGAAGACCTTCATCTTCCGTGTGGATCGTTTCTCTAAGCCCTCTGTTTTTCCGACCCATTACCACTGGTACACTTCTGAGTTTGCAGAATCCCACAAAATCCTCCATGTTTACGACACCGTTTTCCATGGATTCTCTGCAACTCTTACTCAACAACAAGTTGATTCCATCGGGAAACACCCTTCTGTGCTTGCCGTTTTTGAGGATCGTCGCCGGCAGCTTCACACCACCCGTTCCCCTCAGTTTCTTGGCCTTCGAAACCAGCGTGGTCTCTGGTCGGATTCTGATTATGGGTCGGATGTAATCATTGGGGTTTTCGACACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGGCGGTGGAAGGGGGTTTGTGAGACGGGAACTAAGTTCACGGCTAGAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCATGAAGCTGGGTCCAACGCTGCTGGTCCGATTATTGGGATTAACGATACGATTGAGTTTAGATCTCCGAGAGATGCCGACGGCCATGGGACTCATACGGCCTCCACGGCGGCTGGACGGCATGCGTTTCAGGCGAGTTTGGAGGGTTTTGCTTCTGGGATAGCGAAGGGTGTGGCTCCTAAAGCGCGTTTGGCGGTTTATAAAGTTTGTTGGAAAAATTCTGGTTGTTTTGATTCTGACATTCTTGCGGCGTTTGATGCGGCGGTTAACGACGGCGTGGATGTCATTTCGATCTCGATTGGAGGCGGCGATGGGGTTTCCTCGCCGTATTATCTCGACCCAATTGCAATCGGAGCGTACGGCGCCGCTTCCAGGGGTATCTTCGTTTCGTCTTCGGCTGGGAATGATGGACCTAATGGAATGTCAGTGACGAACTTGGCGCCGTGGGTTACGACCGTTGGAGCCGGTACGATTGACCGTAATTTCCCGGCGGTGGTGACTCTGGGTAACGGACGGAGGATTTCCGGCGTGTCACTCTACGCCGGAGCGCCTTTGAACGGTACAATGTTTCCATTGGTTTACCCTGGGAAATCAGGGGTGCTCTCTGTTTCGCTCTGTATGGATAACTCTCTCGATCCTAAGGTTGTTGCCGGAAAAATTGTGATCTGCGACAGGGGAAGTAGTCCCAGAGTGGCTAAGGGTTTGGTCGTTAAGAAAGCTGGCGGCGTCGGAATGATTCTGGCGAATGGAATCTCAAACGGCGAAGGACTCGTCGGCGATGCCCATCTTCTCCCCGCCTGCGCAGTCGGCTCCGACGAAGGCGATTCCATGAAAGCCTACGCATCGTCCTCTCCAAATCCCACAGCTACCATCGCCTTCCAGGGCACTATAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCCTTCTCAGCAAGAGGGCCAAATGGATTAAACCCAGAAATTCTAAAACCAGACATAATCGCACCAGGGGTTAACATTCTCGCCGCCTGGACCGACGCTGTCGGTCCAACCGGTTTGGACTTTGATACAAGAAAAACAGAGTTCAACATCTTGTCTGGTACATCAATGGCGTGTCCTCATGTAAGTGGTGCCGCTGCTCTGTTGAAATCAGCTCACCCAGATTGGAGTCCAGCGGCGTTAAGATCCGCCATGATGACCACCGCAAGTACCACCGACAACCGACGACAGCCGATGACGGAAGAATCCACCGGAAAAGCTTCAACTCCGTACGATTTCGGTGCAGGGCATGTAAATTTGGGTCTAGCAATGGACCCTGGTTTAGTCTACGACATTACAAACACGGATTACATCAACTTCTTGTGCTCAATCGGATACGGACCGAAGATGATTCAAGTGATAACAAGAACGCCGGTAACCTGTCCGGCGAAAAAACCATTGCCGGAAAATCTCAATTACCCATCAATCGTTGCAGTATTCTCCAGTCTATCAAAAGGGTGGTCGACAAAATCGTTTATCCGAACAGTGACGAACGTGGGTCCTGCCAATTCGGTGTACCGTGCCAAAATCGAAGCTCCAAAAGGGGTAACGGTGAAGGTAAAGCCTTCGAAGCTCGTGTTCTCAGCGGCGACGAAGAAACAGAGCTTCGTTGTAGCAGTGTCAGCCGACAACCAGAATCTAGCACTGGGCGACGTGGGCGCTGTTTTTGGTTGGGTTTCTTGGTCGGATGGAAAGCATGTGGTCCGAAGCCCACTCGTAGTAACCCAATTAGAGCCGTTGTAA

Protein sequence

MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL
Homology
BLAST of CmoCh20G007530 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 570/760 (75.00%), Postives = 658/760 (86.58%), Query Frame = 0

Query: 17  LLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYDTVFHGF 76
           L +   F   A S   KTFIFR+D  S PS+FPTHYHWY++EFAE  +I+HVY TVFHGF
Sbjct: 10  LFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTVFHGF 69

Query: 77  SATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGV 136
           SA +T  + D++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGSDVIIGV
Sbjct: 70  SAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIGV 129

Query: 137 FDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHEAGSNAA 196
           FDTGI PERRSFSD+NLGPIP+RW+GVCE+G +F+ RNCNRKI+GARFF+KG +     A
Sbjct: 130 FDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQ-----A 189

Query: 197 GPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKARLAVYK 256
             I GIN T+EF SPRDADGHGTHT+STAAGRHAF+AS+ G+ASG+AKGVAPKAR+A YK
Sbjct: 190 AVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYK 249

Query: 257 VCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGIF 316
           VCWK+SGC DSDILAAFDAAV DGVDVISISIGGGDG++SPYYLDPIAIG+YGAAS+GIF
Sbjct: 250 VCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIF 309

Query: 317 VSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAGAPLNGT 376
           VSSSAGN+GPNGMSVTNLAPWVTTVGA TIDRNFPA   LG+G R+ GVSLYAG PLNG 
Sbjct: 310 VSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGR 369

Query: 377 MFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGGVGMILA 436
           MFP+VYPGKSG+ S SLCM+N+LDPK V GKIVICDRGSSPRVAKGLVVKKAGGVGMILA
Sbjct: 370 MFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILA 429

Query: 437 NGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIKPAPVVAS 496
           NG SNGEGLVGDAHL+PACAVGS+EGD +KAYASS PNP A+I F+GTI+GIKPAPV+AS
Sbjct: 430 NGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIAS 489

Query: 497 FSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHV 556
           FS RGPNGL+PEILKPD+IAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTSMACPHV
Sbjct: 490 FSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHV 549

Query: 557 SGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAGHVNLGL 616
           SGAAALLKSAHPDWSPA +RSAMMTT +  DN  + + +ESTGK++TPYD+G+GH+NLG 
Sbjct: 550 SGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGR 609

Query: 617 AMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCP-AKKPLPENLNYPSIVAVFS 676
           AM+PGLVYDITN DYI FLCSIGYGPK IQVITRTPV CP  +KP P NLNYPSI AVF 
Sbjct: 610 AMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFP 669

Query: 677 SLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQSFVVAVS 736
           +  +G  +K+ IRT TNVG A +VYRA+IE+P+GVTV VKP +LVF++A K++S+ V V+
Sbjct: 670 TNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVT 729

Query: 737 ADNQNLALGDVGAVFGWVSWSD-GKHVVRSPLVVTQLEPL 775
            + +N+ LG+ GAVFG V+W D GKHVVRSP+VVTQ++ L
Sbjct: 730 VNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of CmoCh20G007530 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 3.2e-248
Identity = 457/783 (58.37%), Postives = 554/783 (70.75%), Query Frame = 0

Query: 10  FFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA----ESHKI 69
           FFF+FFFLL +   + SA S    T+I  VD  +KPS+FPTH+HWYTS  A        I
Sbjct: 3   FFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSI 62

Query: 70  LHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLW 129
           +H YDTVFHGFSA LT Q    +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL 
Sbjct: 63  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 122

Query: 130 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGAR 189
            +SD+GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGAR
Sbjct: 123 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 182

Query: 190 FFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIA 249
           FF  G+EA +        +N+T EFRSPRD+DGHGTHTAS +AGR+ F AS  G+A G+A
Sbjct: 183 FFCGGYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 242

Query: 250 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPI 309
            G+APKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD I
Sbjct: 243 AGMAPKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAI 302

Query: 310 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRIS 369
           AIGA+GA  RGIFVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ IS
Sbjct: 303 AIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMIS 362

Query: 370 GVSLYAGAPLN-GTMFPLVYPGK---SGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRV 429
           GVS+Y G  L+ G M+PLVY G        S SLC++ SLDP +V GKIV+CDRG + R 
Sbjct: 363 GVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRA 422

Query: 430 AKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYAS------SSP 489
            KG +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS 
Sbjct: 423 TKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSK 482

Query: 490 NPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTG 549
           +PTATI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G
Sbjct: 483 HPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 542

Query: 550 LDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPM 609
           +  D R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA T DN  +PM
Sbjct: 543 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 602

Query: 610 TEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPV 669
            +ESTG  S+  D+G+GHV+   AMDPGLVYDIT+ DYINFLC+  Y    I  ITR   
Sbjct: 603 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 662

Query: 670 TCPAKKPLPE--NLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVT 729
            C   +      NLNYPS   VF    +   +  FIRTVTNVG ++SVY  KI  P+G T
Sbjct: 663 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 722

Query: 730 VKVKPSKLVFSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQL 775
           V V+P KL F    +K SFVV V      L+ G      G + WSDGK  V SPLVVT  
Sbjct: 723 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 775

BLAST of CmoCh20G007530 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 2.4e-211
Identity = 394/763 (51.64%), Postives = 524/763 (68.68%), Query Frame = 0

Query: 14  FFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTS---EFAESHKILHVYD 73
           FF LL +    VS+ S  + T+I  + +   PS F  H +WY S     ++S ++L+ Y+
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYE 70

Query: 74  TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGLWSDSDYG 133
              HGFS  LTQ++ DS+   P V++V  + R +LHTTR+P FLGL  +   L+ ++   
Sbjct: 71  NAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSY 130

Query: 134 SDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGH 193
           SDV++GV DTG+ PE +S+SD   GPIP  WKG CE GT FTA  CNRK++GARFF++G+
Sbjct: 131 SDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGY 190

Query: 194 EAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPK 253
           E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL G+ASG A+G+AP+
Sbjct: 191 E---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPR 250

Query: 254 ARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYG 313
           AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S YY D +AIGA+ 
Sbjct: 251 ARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDGVAIGAFA 310

Query: 314 AASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYA 373
           A  RGI VS SAGN GP+  S++N+APW+TTVGAGT+DR+FPA+  LGNG+  +GVSL+ 
Sbjct: 311 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 370

Query: 374 GAPLNGTMFPLVYPGK-SGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKA 433
           G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV KG VVK A
Sbjct: 371 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 430

Query: 434 GGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGI 493
           GGVGMILAN  +NGE LV DAHLLPA  VG   GD ++ Y ++ PNPTA+I+  GT++G+
Sbjct: 431 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGV 490

Query: 494 KPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSG 553
           KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT A GPTGL  D+R+ EFNI+SG
Sbjct: 491 KPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 550

Query: 554 TSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFG 613
           TSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T    +P+ + +TGK STP+D G
Sbjct: 551 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 610

Query: 614 AGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTC-PAKKPLPENLNY 673
           AGHV+   A +PGL+YD+T  DY+ FLC++ Y    I+ ++R   TC P+K     +LNY
Sbjct: 611 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNY 670

Query: 674 PSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEA-PKGVTVKVKPSKLVFSAATK 733
           PS    F+    G     + RTVT+VG A + Y  K+ +   GV + V+P+ L F  A +
Sbjct: 671 PS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNFKEANE 730

Query: 734 KQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVT 770
           K+S+ V  + D+   +  +    FG + WSDGKHVV SP+ ++
Sbjct: 731 KKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmoCh20G007530 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 3.6e-207
Identity = 379/754 (50.27%), Postives = 505/754 (66.98%), Query Frame = 0

Query: 15  FFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYDTVFH 74
           F  L++H       +  KKT+I RV+   KP  F TH+ WYTS+      +L+ Y T FH
Sbjct: 17  FLFLLLH-------TTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYTTSFH 76

Query: 75  GFSATLTQQQVDS-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVI 134
           GFSA L   + DS +    S+L +FED    LHTTR+P+FLGL ++ G+       + VI
Sbjct: 77  GFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVI 136

Query: 135 IGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHEAGS 194
           IGV DTG+ PE RSF D ++  IP +WKG CE+G+ F ++ CN+K++GAR FSKG +  S
Sbjct: 137 IGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMAS 196

Query: 195 NAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKARLA 254
                  G +   E  SPRD DGHGTHT++TAAG     AS  G+A+G A+G+A +AR+A
Sbjct: 197 GG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVA 256

Query: 255 VYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASR 314
            YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D IAIGA+ A  R
Sbjct: 257 TYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRDTIAIGAFSAMER 316

Query: 315 GIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAGAPL 374
           G+FVS SAGN GP   SV N+APWV TVGAGT+DR+FPA   LGNG+R++GVSLY+G  +
Sbjct: 317 GVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGM 376

Query: 375 NGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGGVGM 434
                 LVY  K    S +LC+  SLD  +V GKIV+CDRG + RV KG VV+ AGG+GM
Sbjct: 377 GTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGM 436

Query: 435 ILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIKPAPV 494
           I+AN  ++GE LV D+HLLPA AVG   GD ++ Y  S   PTA + F+GT++ +KP+PV
Sbjct: 437 IMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPV 496

Query: 495 VASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMAC 554
           VA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T+FNI+SGTSM+C
Sbjct: 497 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 556

Query: 555 PHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAGHVN 614
           PH+SG A LLK+AHP+WSP+A++SA+MTTA   DN   P+ + +    S PY  G+GHV+
Sbjct: 557 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 616

Query: 615 LGLAMDPGLVYDITNTDYINFLCSIGYG-PKMIQVITRTPVTCPAKKPLPENLNYPSIVA 674
              A+ PGLVYDI+  +YI FLCS+ Y    ++ ++ R  V C  K   P  LNYPS   
Sbjct: 617 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPS--- 676

Query: 675 VFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQSFVV 734
            FS L  G     + R VTNVG A+SVY+  +     V + VKPSKL F +  +K+ + V
Sbjct: 677 -FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTV 736

Query: 735 AVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPL 767
                 + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 737 TF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of CmoCh20G007530 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 1.1e-197
Identity = 381/788 (48.35%), Postives = 507/788 (64.34%), Query Frame = 0

Query: 13  FFFFLLIVHRFTVSADS----PLKKTFIFRVDRFSKPSVFPTHYHWYTS----------- 72
           F F +L ++   + A++      KKT++  +D+ + P  +  H  WY+S           
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 73  -EFAESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL 132
            E   +++IL+ Y T FHG +A LTQ++ + + +   V+AV  + R +LHTTRSP FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 133 RNQRG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARN 192
             Q    +W++     DV++GV DTGI PE  SF+D  + P+P  W+G CETG +F  RN
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 193 CNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQAS 252
           CNRKIVGAR F +G+EA +        I++ +E++SPRD DGHGTHTA+T AG     A+
Sbjct: 191 CNRKIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGAN 250

Query: 253 LEGFASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGV 312
           L GFA G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG   
Sbjct: 251 LFGFAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG--- 310

Query: 313 SSPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVV 372
            S Y  D ++I  +GA   G+FVS SAGN GP+ +S+TN++PW+TTVGA T+DR+FPA V
Sbjct: 311 VSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATV 370

Query: 373 TLGNGRRISGVSLYAGAPL--NGTMFPLVYPGK--SGVLSVSLCMDNSLDPKVVAGKIVI 432
            +G  R   GVSLY G  +      +PLVY G+  S     S C+D +LD + VAGKIVI
Sbjct: 371 KIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVI 430

Query: 433 CDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYAS 492
           CDRG +PRV KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA 
Sbjct: 431 CDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAM 490

Query: 493 SSPNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVG 552
           +S   TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + 
Sbjct: 491 TSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMA 550

Query: 553 PTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRR 612
           P+ L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA   DN  
Sbjct: 551 PSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMF 610

Query: 613 QPMTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITR 672
           +P+T+ S    S+PYD GAGH++   A DPGLVYDI   +Y  FLC+    P  ++V T+
Sbjct: 611 KPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTK 670

Query: 673 -TPVTCP---AKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEA 732
            +  TC    AK   P NLNYP+I A+F   +      +  RTVTNVGP  S Y+  +  
Sbjct: 671 HSNRTCKHTLAKN--PGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSP 730

Query: 733 PKGVTVKVKPSKLVFSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPL 775
            KG +V V+P  L F++  +K S+ V      +          FG + W    H VRSP+
Sbjct: 731 FKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPV 780

BLAST of CmoCh20G007530 vs. ExPASy TrEMBL
Match: A0A6J1FYE5 (subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE=3 SV=1)

HSP 1 Score: 1535.0 bits (3973), Expect = 0.0e+00
Identity = 774/774 (100.00%), Postives = 774/774 (100.00%), Query Frame = 0

Query: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA
Sbjct: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240
           GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420
           RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 775
           FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

BLAST of CmoCh20G007530 vs. ExPASy TrEMBL
Match: A0A6J1JEF5 (subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3 SV=1)

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 763/774 (98.58%), Postives = 765/774 (98.84%), Query Frame = 0

Query: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAAYLSPL    FFFFLLIVHR TVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA
Sbjct: 1   MAAYLSPL----FFFFLLIVHRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240
           GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAASRGIFVSSS GNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420
           RISGVSLYAGAPLN TMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYGPKMIQVITRTPVTCPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 775
           FSAA KKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAAMKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

BLAST of CmoCh20G007530 vs. ExPASy TrEMBL
Match: A0A1S3C7D0 (subtilisin-like protease SBT1.6 OS=Cucumis melo OX=3656 GN=LOC103497323 PE=3 SV=1)

HSP 1 Score: 1434.9 bits (3713), Expect = 0.0e+00
Identity = 723/774 (93.41%), Postives = 747/774 (96.51%), Query Frame = 0

Query: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAA+LS     FFF FLL++   TVS+ SPL KTFI R+DRFSKPSVFPTHYHWYTSEF 
Sbjct: 1   MAAFLSTSLSPFFFSFLLLLS--TVSSLSPL-KTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           +S +ILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA+NCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240
           GARFFSKGHEAG+NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRH+FQASLEG+AS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAAS+G+FVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGNGR
Sbjct: 301 DPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420
           +I GVSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPKVV GKIVICDRGSSPRVA
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGL PEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS  DNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
            STPYDFGAGHVNLGLAMDPGL+YDITNTDYINFLCSIGYGPKMIQVITRTPV CP KKP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGP+NSVYR KIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 775
           FS   KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of CmoCh20G007530 vs. ExPASy TrEMBL
Match: A0A0A0LJ58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1)

HSP 1 Score: 1434.1 bits (3711), Expect = 0.0e+00
Identity = 721/774 (93.15%), Postives = 745/774 (96.25%), Query Frame = 0

Query: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAA+LS     FFF FLL++   TVS+ SPL KTFI R+DRFSKPSVFPTHYHWYTSEF 
Sbjct: 1   MAAFLSTSLSSFFFSFLLLLS--TVSSHSPL-KTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           +S +ILHVYDTVFHGFSATLTQ QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA+NCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240
           GARFFSKGHEAG+NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRH+FQASLEG+AS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIG+YGAAS+G+FVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGNGR
Sbjct: 301 DPIAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420
           +I GVSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPKVV GKIVICDRGSSPRVA
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFD RK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
            STPYDFGAGHVNLGLAMDPGL+YDITNTDYINFLCSIGYGPKMIQVITRTPV CP KKP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIV VFSSLSKGWSTKSFIRT TNVGP+NSVYR KIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 775
           FS   KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of CmoCh20G007530 vs. ExPASy TrEMBL
Match: A0A5D3BLE2 (Subtilisin-like protease SBT1.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001210 PE=3 SV=1)

HSP 1 Score: 1421.4 bits (3678), Expect = 0.0e+00
Identity = 705/741 (95.14%), Postives = 725/741 (97.84%), Query Frame = 0

Query: 34  TFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYDTVFHGFSATLTQQQVDSIGKHPS 93
           TFI R+DRFSKPSVFPTHYHWYTSEF +S +ILHVYDTVFHGFSATLTQ+QVDSIGKHPS
Sbjct: 2   TFIVRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQEQVDSIGKHPS 61

Query: 94  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 153
           VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL
Sbjct: 62  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 121

Query: 154 GPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEFRSPRD 213
           GPIPRRWKGVCETGTKFTA+NCNRKIVGARFFSKGHEAG+NAAGPIIGINDTIE+RSPRD
Sbjct: 122 GPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD 181

Query: 214 ADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 273
           ADGHGTHTASTAAGRH+FQASLEG+ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF
Sbjct: 182 ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 241

Query: 274 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTN 333
           DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS+G+FVSSSAGNDGPNGMSVTN
Sbjct: 242 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN 301

Query: 334 LAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSL 393
           LAPWVTTVGAGTIDRNFP+VVTLGNGR+I GVSLYAGAPLNGTM+PLVYPGKSGVLSVSL
Sbjct: 302 LAPWVTTVGAGTIDRNFPSVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 361

Query: 394 CMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 453
           CM+NSLDPKVV GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP
Sbjct: 362 CMENSLDPKVVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 421

Query: 454 ACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPD 513
           ACAVGSDEGD+MKAYASSS NPTATIAFQGTIIGIKPAPVVASFSARGPNGL PEILKPD
Sbjct: 422 ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLTPEILKPD 481

Query: 514 IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 573
           IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA
Sbjct: 482 IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 541

Query: 574 ALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYIN 633
           ALRSAMMTTAS  DNRRQPMTEESTGK STPYDFGAGHVNLGLAMDPGL+YDITNTDYIN
Sbjct: 542 ALRSAMMTTASIIDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYIN 601

Query: 634 FLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNV 693
           FLCSIGYGPKMIQVITRTPV CP KKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNV
Sbjct: 602 FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNV 661

Query: 694 GPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQSFVVAVSADNQNLALGDVGAVFGWV 753
           GP+NSVYR KIEAPKGVTVKVKPSKLVFS   KKQSFVVA+SADNQNLALGDVGAVFGW+
Sbjct: 662 GPSNSVYRVKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL 721

Query: 754 SWSDGKHVVRSPLVVTQLEPL 775
           SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 SWSDGKHVVRSPLVVTQLEPL 742

BLAST of CmoCh20G007530 vs. NCBI nr
Match: XP_022943985.1 (subtilisin-like protease SBT1.6 [Cucurbita moschata])

HSP 1 Score: 1535.0 bits (3973), Expect = 0.0e+00
Identity = 774/774 (100.00%), Postives = 774/774 (100.00%), Query Frame = 0

Query: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA
Sbjct: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240
           GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420
           RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 775
           FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

BLAST of CmoCh20G007530 vs. NCBI nr
Match: KAG6571024.1 (Subtilisin-like protease 1.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1526.9 bits (3952), Expect = 0.0e+00
Identity = 772/774 (99.74%), Postives = 772/774 (99.74%), Query Frame = 0

Query: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAAYLSPL  FFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA
Sbjct: 1   MAAYLSPL--FFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240
           GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420
           RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 775
           FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 772

BLAST of CmoCh20G007530 vs. NCBI nr
Match: KAG7010855.1 (Subtilisin-like protease SBT1.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1525.4 bits (3948), Expect = 0.0e+00
Identity = 771/774 (99.61%), Postives = 771/774 (99.61%), Query Frame = 0

Query: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAAYLSPL   FFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA
Sbjct: 1   MAAYLSPL---FFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240
           GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420
           RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 775
           FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 771

BLAST of CmoCh20G007530 vs. NCBI nr
Match: XP_022985703.1 (subtilisin-like protease SBT1.6 [Cucurbita maxima])

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 763/774 (98.58%), Postives = 765/774 (98.84%), Query Frame = 0

Query: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAAYLSPL    FFFFLLIVHR TVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA
Sbjct: 1   MAAYLSPL----FFFFLLIVHRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240
           GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAASRGIFVSSS GNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420
           RISGVSLYAGAPLN TMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYGPKMIQVITRTPVTCPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 775
           FSAA KKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAAMKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

BLAST of CmoCh20G007530 vs. NCBI nr
Match: XP_023512905.1 (subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1507.7 bits (3902), Expect = 0.0e+00
Identity = 763/774 (98.58%), Postives = 767/774 (99.10%), Query Frame = 0

Query: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAAYLSPL    FFFF+LIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWY+SEFA
Sbjct: 1   MAAYLSPL---VFFFFVLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYSSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240
           GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420
           RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKI+APKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIQAPKGVTVKVKPSKLV 720

Query: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 775
           FSAA KKQSFVVAVSADNQNLALGDVGAVFG VSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAAMKKQSFVVAVSADNQNLALGDVGAVFGCVSWSDGKHVVRSPLVVTQLEPL 771

BLAST of CmoCh20G007530 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 570/760 (75.00%), Postives = 658/760 (86.58%), Query Frame = 0

Query: 17  LLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYDTVFHGF 76
           L +   F   A S   KTFIFR+D  S PS+FPTHYHWY++EFAE  +I+HVY TVFHGF
Sbjct: 10  LFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTVFHGF 69

Query: 77  SATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGV 136
           SA +T  + D++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGSDVIIGV
Sbjct: 70  SAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIGV 129

Query: 137 FDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHEAGSNAA 196
           FDTGI PERRSFSD+NLGPIP+RW+GVCE+G +F+ RNCNRKI+GARFF+KG +     A
Sbjct: 130 FDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQ-----A 189

Query: 197 GPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKARLAVYK 256
             I GIN T+EF SPRDADGHGTHT+STAAGRHAF+AS+ G+ASG+AKGVAPKAR+A YK
Sbjct: 190 AVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYK 249

Query: 257 VCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGIF 316
           VCWK+SGC DSDILAAFDAAV DGVDVISISIGGGDG++SPYYLDPIAIG+YGAAS+GIF
Sbjct: 250 VCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIF 309

Query: 317 VSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAGAPLNGT 376
           VSSSAGN+GPNGMSVTNLAPWVTTVGA TIDRNFPA   LG+G R+ GVSLYAG PLNG 
Sbjct: 310 VSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGR 369

Query: 377 MFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGGVGMILA 436
           MFP+VYPGKSG+ S SLCM+N+LDPK V GKIVICDRGSSPRVAKGLVVKKAGGVGMILA
Sbjct: 370 MFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILA 429

Query: 437 NGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIKPAPVVAS 496
           NG SNGEGLVGDAHL+PACAVGS+EGD +KAYASS PNP A+I F+GTI+GIKPAPV+AS
Sbjct: 430 NGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIAS 489

Query: 497 FSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHV 556
           FS RGPNGL+PEILKPD+IAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTSMACPHV
Sbjct: 490 FSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHV 549

Query: 557 SGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAGHVNLGL 616
           SGAAALLKSAHPDWSPA +RSAMMTT +  DN  + + +ESTGK++TPYD+G+GH+NLG 
Sbjct: 550 SGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGR 609

Query: 617 AMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCP-AKKPLPENLNYPSIVAVFS 676
           AM+PGLVYDITN DYI FLCSIGYGPK IQVITRTPV CP  +KP P NLNYPSI AVF 
Sbjct: 610 AMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFP 669

Query: 677 SLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQSFVVAVS 736
           +  +G  +K+ IRT TNVG A +VYRA+IE+P+GVTV VKP +LVF++A K++S+ V V+
Sbjct: 670 TNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVT 729

Query: 737 ADNQNLALGDVGAVFGWVSWSD-GKHVVRSPLVVTQLEPL 775
            + +N+ LG+ GAVFG V+W D GKHVVRSP+VVTQ++ L
Sbjct: 730 VNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of CmoCh20G007530 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 859.4 bits (2219), Expect = 2.3e-249
Identity = 457/783 (58.37%), Postives = 554/783 (70.75%), Query Frame = 0

Query: 10  FFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA----ESHKI 69
           FFF+FFFLL +   + SA S    T+I  VD  +KPS+FPTH+HWYTS  A        I
Sbjct: 3   FFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSI 62

Query: 70  LHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLW 129
           +H YDTVFHGFSA LT Q    +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL 
Sbjct: 63  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 122

Query: 130 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGAR 189
            +SD+GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGAR
Sbjct: 123 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 182

Query: 190 FFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIA 249
           FF  G+EA +        +N+T EFRSPRD+DGHGTHTAS +AGR+ F AS  G+A G+A
Sbjct: 183 FFCGGYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 242

Query: 250 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPI 309
            G+APKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD I
Sbjct: 243 AGMAPKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAI 302

Query: 310 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRIS 369
           AIGA+GA  RGIFVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ IS
Sbjct: 303 AIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMIS 362

Query: 370 GVSLYAGAPLN-GTMFPLVYPGK---SGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRV 429
           GVS+Y G  L+ G M+PLVY G        S SLC++ SLDP +V GKIV+CDRG + R 
Sbjct: 363 GVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRA 422

Query: 430 AKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYAS------SSP 489
            KG +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS 
Sbjct: 423 TKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSK 482

Query: 490 NPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTG 549
           +PTATI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G
Sbjct: 483 HPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 542

Query: 550 LDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPM 609
           +  D R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA T DN  +PM
Sbjct: 543 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 602

Query: 610 TEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPV 669
            +ESTG  S+  D+G+GHV+   AMDPGLVYDIT+ DYINFLC+  Y    I  ITR   
Sbjct: 603 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 662

Query: 670 TCPAKKPLPE--NLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVT 729
            C   +      NLNYPS   VF    +   +  FIRTVTNVG ++SVY  KI  P+G T
Sbjct: 663 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 722

Query: 730 VKVKPSKLVFSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQL 775
           V V+P KL F    +K SFVV V      L+ G      G + WSDGK  V SPLVVT  
Sbjct: 723 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 775

BLAST of CmoCh20G007530 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 736.9 bits (1901), Expect = 1.7e-212
Identity = 394/763 (51.64%), Postives = 524/763 (68.68%), Query Frame = 0

Query: 14  FFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTS---EFAESHKILHVYD 73
           FF LL +    VS+ S  + T+I  + +   PS F  H +WY S     ++S ++L+ Y+
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYE 70

Query: 74  TVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGLWSDSDYG 133
              HGFS  LTQ++ DS+   P V++V  + R +LHTTR+P FLGL  +   L+ ++   
Sbjct: 71  NAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSY 130

Query: 134 SDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGH 193
           SDV++GV DTG+ PE +S+SD   GPIP  WKG CE GT FTA  CNRK++GARFF++G+
Sbjct: 131 SDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGY 190

Query: 194 EAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPK 253
           E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL G+ASG A+G+AP+
Sbjct: 191 E---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPR 250

Query: 254 ARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYG 313
           AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S YY D +AIGA+ 
Sbjct: 251 ARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDGVAIGAFA 310

Query: 314 AASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYA 373
           A  RGI VS SAGN GP+  S++N+APW+TTVGAGT+DR+FPA+  LGNG+  +GVSL+ 
Sbjct: 311 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 370

Query: 374 GAPLNGTMFPLVYPGK-SGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKA 433
           G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV KG VVK A
Sbjct: 371 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 430

Query: 434 GGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGI 493
           GGVGMILAN  +NGE LV DAHLLPA  VG   GD ++ Y ++ PNPTA+I+  GT++G+
Sbjct: 431 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGV 490

Query: 494 KPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSG 553
           KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT A GPTGL  D+R+ EFNI+SG
Sbjct: 491 KPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 550

Query: 554 TSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFG 613
           TSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T    +P+ + +TGK STP+D G
Sbjct: 551 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 610

Query: 614 AGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTC-PAKKPLPENLNY 673
           AGHV+   A +PGL+YD+T  DY+ FLC++ Y    I+ ++R   TC P+K     +LNY
Sbjct: 611 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNY 670

Query: 674 PSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEA-PKGVTVKVKPSKLVFSAATK 733
           PS    F+    G     + RTVT+VG A + Y  K+ +   GV + V+P+ L F  A +
Sbjct: 671 PS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNFKEANE 730

Query: 734 KQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVT 770
           K+S+ V  + D+   +  +    FG + WSDGKHVV SP+ ++
Sbjct: 731 KKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmoCh20G007530 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 723.0 bits (1865), Expect = 2.5e-208
Identity = 379/754 (50.27%), Postives = 505/754 (66.98%), Query Frame = 0

Query: 15  FFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYDTVFH 74
           F  L++H       +  KKT+I RV+   KP  F TH+ WYTS+      +L+ Y T FH
Sbjct: 17  FLFLLLH-------TTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYTTSFH 76

Query: 75  GFSATLTQQQVDS-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVI 134
           GFSA L   + DS +    S+L +FED    LHTTR+P+FLGL ++ G+       + VI
Sbjct: 77  GFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVI 136

Query: 135 IGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHEAGS 194
           IGV DTG+ PE RSF D ++  IP +WKG CE+G+ F ++ CN+K++GAR FSKG +  S
Sbjct: 137 IGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMAS 196

Query: 195 NAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKARLA 254
                  G +   E  SPRD DGHGTHT++TAAG     AS  G+A+G A+G+A +AR+A
Sbjct: 197 GG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVA 256

Query: 255 VYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASR 314
            YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D IAIGA+ A  R
Sbjct: 257 TYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRDTIAIGAFSAMER 316

Query: 315 GIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAGAPL 374
           G+FVS SAGN GP   SV N+APWV TVGAGT+DR+FPA   LGNG+R++GVSLY+G  +
Sbjct: 317 GVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGM 376

Query: 375 NGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGGVGM 434
                 LVY  K    S +LC+  SLD  +V GKIV+CDRG + RV KG VV+ AGG+GM
Sbjct: 377 GTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGM 436

Query: 435 ILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIKPAPV 494
           I+AN  ++GE LV D+HLLPA AVG   GD ++ Y  S   PTA + F+GT++ +KP+PV
Sbjct: 437 IMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPV 496

Query: 495 VASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMAC 554
           VA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T+FNI+SGTSM+C
Sbjct: 497 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 556

Query: 555 PHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAGHVN 614
           PH+SG A LLK+AHP+WSP+A++SA+MTTA   DN   P+ + +    S PY  G+GHV+
Sbjct: 557 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 616

Query: 615 LGLAMDPGLVYDITNTDYINFLCSIGYG-PKMIQVITRTPVTCPAKKPLPENLNYPSIVA 674
              A+ PGLVYDI+  +YI FLCS+ Y    ++ ++ R  V C  K   P  LNYPS   
Sbjct: 617 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPS--- 676

Query: 675 VFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQSFVV 734
            FS L  G     + R VTNVG A+SVY+  +     V + VKPSKL F +  +K+ + V
Sbjct: 677 -FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTV 736

Query: 735 AVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPL 767
                 + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 737 TF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of CmoCh20G007530 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 691.4 bits (1783), Expect = 8.2e-199
Identity = 381/788 (48.35%), Postives = 507/788 (64.34%), Query Frame = 0

Query: 13  FFFFLLIVHRFTVSADS----PLKKTFIFRVDRFSKPSVFPTHYHWYTS----------- 72
           F F +L ++   + A++      KKT++  +D+ + P  +  H  WY+S           
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 73  -EFAESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL 132
            E   +++IL+ Y T FHG +A LTQ++ + + +   V+AV  + R +LHTTRSP FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 133 RNQRG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARN 192
             Q    +W++     DV++GV DTGI PE  SF+D  + P+P  W+G CETG +F  RN
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 193 CNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQAS 252
           CNRKIVGAR F +G+EA +        I++ +E++SPRD DGHGTHTA+T AG     A+
Sbjct: 191 CNRKIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGAN 250

Query: 253 LEGFASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGV 312
           L GFA G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG   
Sbjct: 251 LFGFAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG--- 310

Query: 313 SSPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVV 372
            S Y  D ++I  +GA   G+FVS SAGN GP+ +S+TN++PW+TTVGA T+DR+FPA V
Sbjct: 311 VSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATV 370

Query: 373 TLGNGRRISGVSLYAGAPL--NGTMFPLVYPGK--SGVLSVSLCMDNSLDPKVVAGKIVI 432
            +G  R   GVSLY G  +      +PLVY G+  S     S C+D +LD + VAGKIVI
Sbjct: 371 KIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVI 430

Query: 433 CDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYAS 492
           CDRG +PRV KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA 
Sbjct: 431 CDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAM 490

Query: 493 SSPNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVG 552
           +S   TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + 
Sbjct: 491 TSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMA 550

Query: 553 PTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRR 612
           P+ L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA   DN  
Sbjct: 551 PSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMF 610

Query: 613 QPMTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITR 672
           +P+T+ S    S+PYD GAGH++   A DPGLVYDI   +Y  FLC+    P  ++V T+
Sbjct: 611 KPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTK 670

Query: 673 -TPVTCP---AKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEA 732
            +  TC    AK   P NLNYP+I A+F   +      +  RTVTNVGP  S Y+  +  
Sbjct: 671 HSNRTCKHTLAKN--PGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSP 730

Query: 733 PKGVTVKVKPSKLVFSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPL 775
            KG +V V+P  L F++  +K S+ V      +          FG + W    H VRSP+
Sbjct: 731 FKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPV 780

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O496070.0e+0075.00Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
Q9LUM33.2e-24858.37Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
O653512.4e-21151.64Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9ZUF63.6e-20750.27Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Q9FLI41.1e-19748.35Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1FYE50.0e+00100.00subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE... [more]
A0A6J1JEF50.0e+0098.58subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3... [more]
A0A1S3C7D00.0e+0093.41subtilisin-like protease SBT1.6 OS=Cucumis melo OX=3656 GN=LOC103497323 PE=3 SV=... [more]
A0A0A0LJ580.0e+0093.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1[more]
A0A5D3BLE20.0e+0095.14Subtilisin-like protease SBT1.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
XP_022943985.10.0e+00100.00subtilisin-like protease SBT1.6 [Cucurbita moschata][more]
KAG6571024.10.0e+0099.74Subtilisin-like protease 1.6, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7010855.10.0e+0099.61Subtilisin-like protease SBT1.6, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022985703.10.0e+0098.58subtilisin-like protease SBT1.6 [Cucurbita maxima][more]
XP_023512905.10.0e+0098.58subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G34980.10.0e+0075.00subtilisin-like serine protease 2 [more]
AT3G14240.12.3e-24958.37Subtilase family protein [more]
AT5G67360.11.7e-21251.64Subtilase family protein [more]
AT2G05920.12.5e-20850.27Subtilase family protein [more]
AT5G51750.18.2e-19948.35subtilase 1.3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 213..226
score: 50.0
coord: 547..563
score: 60.83
coord: 129..148
score: 32.4
IPR003137PA domainPFAMPF02225PAcoord: 379..464
e-value: 5.7E-9
score: 35.9
NoneNo IPR availableGENE3D3.50.30.30coord: 347..486
e-value: 3.8E-179
score: 598.3
NoneNo IPR availableGENE3D2.60.40.2310coord: 635..771
e-value: 4.2E-41
score: 141.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 582..601
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 582..602
NoneNo IPR availablePANTHERPTHR10795:SF675SUBTILISIN-LIKE PROTEASE SBT1.7coord: 22..768
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 104..618
score: 30.866398
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 353..479
e-value: 5.51686E-40
score: 141.397
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 404..473
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 51..102
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 129..590
e-value: 2.9E-47
score: 162.1
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 22..106
e-value: 4.2E-13
score: 51.6
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 131..631
e-value: 3.8E-179
score: 598.3
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 105..622
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 48..106
e-value: 3.7E-10
score: 40.3
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 664..768
e-value: 2.1E-29
score: 101.6
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 22..768
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 548..558
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 104..584
e-value: 5.94331E-145
score: 425.474

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G007530.1CmoCh20G007530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity