CmoCh20G007290 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh20G007290 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 2.0e-281
Identity = 545/923 (59.05%), Postives = 676/923 (73.24%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGE- 109
           MAV DA+DR+EVIVK+D  + ++N       S  + G+IWR+ SYDFW D +   N G  
Sbjct: 1   MAV-DAADRREVIVKIDGENGNNNG-----VSGETVGKIWRDGSYDFWTDGEGNLNKGHN 60

Query: 110 ----------GSNGDGGKGEDFEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEM 169
                      + G+  K E FEFR+     EDPP+KLIGQFLHKQ+ASGE+CLDMD+ M
Sbjct: 61  AAAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGM 120

Query: 170 LELPQDKTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPL 229
            EL Q + L  V+ESP R S+K                         PV           
Sbjct: 121 DEL-QSRGLTPVSESP-RVSTKR-----------------------DPVGR--------- 180

Query: 230 RDHRPHGNGHDDGAAEVVSCTSNSSFERNVSFQRKSS-LLKTKTKSRLLDPPEHQ----- 289
           RD R + N +DDG  EVV C+ N     N   QR SS LLK +T+SRL DPP  Q     
Sbjct: 181 RDSRSNTNNNDDG--EVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQT 240

Query: 290 -DRRSGRVPKSGQIRSGLLSKAL----DEEDDDPFLEEDLPDEYKKANLGVLTMLQWASL 349
            D +SGR+PKSGQ++SG   K+     +EE+DDPF  EDLP+EY+K  L +  +L+W SL
Sbjct: 241 ADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSL 300

Query: 350 ILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLL 409
           ILIIA  +CTL I   R+KKLW+L+LWKWE M+LVLICGRL S W ++I+VFFIER+FLL
Sbjct: 301 ILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLL 360

Query: 410 RKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTL 469
           RKRVLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV +   + AL  VTK+ +CLLV  L
Sbjct: 361 RKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFL 420

Query: 470 VWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKNKEEEVKLAEEV 529
           +WLVKTL+VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPPLIEIQKN+EEE +++ EV
Sbjct: 421 LWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEV 480

Query: 530 IKLQNAGATIPPDLRASAFSSPEGGRVIGSGGLQKSP--RGRSAKLSRALSKKG-----D 589
            K QN G                   V    G QKSP   G+S  LS  LS  G     +
Sbjct: 481 KKFQNPGG------------------VEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGEN 540

Query: 590 EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSEYEAK 649
           +GITID LH+L+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D + +D+   QI+SE+EAK
Sbjct: 541 KGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAK 600

Query: 650 VAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNWVVNA 709
           +AA++IF NVA  GSK+IY  D+MRF+ +DEA KT+SLFEGASE+ +ISKSSLKNWVVNA
Sbjct: 601 LAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNA 660

Query: 710 FRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVA 769
           FRERRALALTLNDTKTAVN+LH+MVNI+V +IILVIWL+ILGI ++KFL+ ++SQ+V+VA
Sbjct: 661 FRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVA 720

Query: 770 FVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPN 829
           F+FGN CK VFE+II+LFV+HPFDVGDRCEIDGVQM+VEEMNILTTVFLRFDNQK+++PN
Sbjct: 721 FIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPN 780

Query: 830 SVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILSYIEGKKEHWCPAPLIIL 889
           S+L TK+I NYYRSPDMGD +EF +HI+TP EKI +++QRI SYIEGKK+HW PAP+I+ 
Sbjct: 781 SLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVF 840

Query: 890 KDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNINVCS 939
           KD+E LN +RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +ELD++YRL PL+INV +
Sbjct: 841 KDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRN 853

BLAST of CmoCh20G007290 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 6.3e-267
Identity = 510/921 (55.37%), Postives = 665/921 (72.20%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEG 109
           MA  D++DR++ IV ++    S    A    S    G IW+ESSYDFW D +  +N+ +G
Sbjct: 1   MAAVDSTDRRDFIVNIN-GQESGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKG 60

Query: 110 SNGDGGKGEDFEFRQQRK------DVEDPPSKLIGQFLHKQKASG-EMCLDMDMEMLELP 169
            + D   G  F FRQ+ +      ++ DPPSKLIGQFLHKQ+ASG E+ LD+++ M EL 
Sbjct: 61  DDED-EDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQ 120

Query: 170 QD-KTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPV----DEEHRVQHPP 229
            +    P  A +  RR    +  S   +    ++D++RRR   + +    DEE       
Sbjct: 121 SNTPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEE------- 180

Query: 230 LRDHRPHGNGHDDGAAEVVSCTSNSSFERNVSFQRKSSLLKTKTKSRLLDPP-------E 289
                    G +   AEV+ C S           +K  L + KTKSRL DPP       +
Sbjct: 181 ---------GRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAID 240

Query: 290 HQDRRSGRVPKSGQIRSGLLSKA-----------LDEEDDDPFLEEDLPDEYKKANLGVL 349
             + +SGR  +SG  +SG L K+            +EE++DPFL+EDLP+E+K+  L   
Sbjct: 241 KTEMKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFW 300

Query: 350 TMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVF 409
             L+W SL+LI+ +L+C+LTI   +RK  WKL+LWKWEV +LVLICGRL S W +RIIVF
Sbjct: 301 VFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVF 360

Query: 410 FIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVL 469
            +E++F  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VL
Sbjct: 361 LVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVL 420

Query: 470 ICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKNKEE 529
           +CLLV+ ++WLVKT++VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPPL+EIQ+ +EE
Sbjct: 421 VCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEE 480

Query: 530 EVKLAEEVIKLQN-AGATIPPDLRASAFSSPEGGRVIGSGGLQKSPRGRSAKLSRALSKK 589
           E ++AE+V  L+  AGA +PP L+A+  S  +               G+S  L+R  SK+
Sbjct: 481 EQQVAEDVKSLEKLAGAKLPPALKATVKSFMK--------------VGKSPGLNRIGSKR 540

Query: 590 GD--EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSE 649
           G+  EGI ID L R++ KNVSAWNMKRLMNI+  GA+STLD+ ++D+  EDE  T I+SE
Sbjct: 541 GEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSE 600

Query: 650 YEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNW 709
           YEAK AA++IF NV   GS+YIYLED +RF+ E+EA + M+LFEGASES KISKS LKNW
Sbjct: 601 YEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNW 660

Query: 710 VVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQL 769
           VV AFRERRALALTLNDTKTAV++LHR++N+++ +II++IWLLILGIAT++FLL ++SQL
Sbjct: 661 VVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQL 720

Query: 770 VLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKI 829
           +LVAFVFGN+CKT+FEAIIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTTVFLR+DNQKI
Sbjct: 721 LLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKI 780

Query: 830 IFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILSYIEGKKEHWCPAP 889
           I+PNSVL TK I NYYRSPDMGDAVEFC+HI+TPPEKI  ++QRILSY++ KK++W PAP
Sbjct: 781 IYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAP 840

Query: 890 LIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNI 934
           +I+   +++LN ++IA+WLTHRMNHQDMGER+ RR  L+EE+ K  +ELD++YRL PLNI
Sbjct: 841 MIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNI 875

BLAST of CmoCh20G007290 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 2.9e-264
Identity = 522/926 (56.37%), Postives = 659/926 (71.17%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFW-NDSDSRRNNGE 109
           MAV     R++ +V++D  D               S + WRESS +FW ND  S+   GE
Sbjct: 1   MAVDSTDQRRDFVVRIDGEDN------------GDSEKFWRESSINFWHNDKSSKPPGGE 60

Query: 110 GSNGDGGKGEDFEFRQQRKDVE-DPPSKLIGQFLHKQKASG-EMCLDMDMEMLELPQDKT 169
             +G      DF  R   K  E DPPSKLI QFL+KQKASG E+ LDM+  M EL Q  T
Sbjct: 61  EDDG----SFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPEL-QKNT 120

Query: 170 LPTVAESPMRRSSKELKVSFESISEISESDSLRRRHR-----------DSPVDEEHRVQH 229
           +P ++ + +  S+  +     +       D++RRR             DS  DEE+RV  
Sbjct: 121 VPPLSSTAVSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRV-- 180

Query: 230 PPLRDHRPHGNGHDDGAAEVVSCTSNSSFERNVSFQRKSSLLKTKTKSRLLDP--PEHQD 289
                         DG +EVV CTSN       S  R  +L+K KT+SRL+DP  P + D
Sbjct: 181 --------------DG-SEVVKCTSNR------STMRTKTLMKMKTRSRLMDPPTPTYPD 240

Query: 290 RRSGRVPKSGQIRSGLL-------------SKALDEEDDDPFLEEDLPDEYKKANLGVLT 349
             SGR P+SG +  G               SK L EE++DPF EEDLP+  +K  + V  
Sbjct: 241 MVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDL-EEEEDPFSEEDLPEGLRKEKICVWV 300

Query: 350 MLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFF 409
           +++W  LILIIA+LIC+L I Y R K LW L LWKWEVM+LVLICGRL S W +++ V+F
Sbjct: 301 IIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYF 360

Query: 410 IERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLI 469
           +E +FL RK+VLYFVYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTKVLI
Sbjct: 361 VESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLI 420

Query: 470 CLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKNKEEE 529
           CLLV+ ++WL+KTL+VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPP IEI     EE
Sbjct: 421 CLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIH---IEE 480

Query: 530 VKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGLQKSPR--GRSAKLSRALSKK 589
            K+A +V   +  G  + P L   A SSP     +GSG LQKSP   G+S  LSR+ SKK
Sbjct: 481 EKVANDVKTFEIVGRKLSP-LGPKAVSSPP-QVTVGSGRLQKSPSRVGKSPVLSRSGSKK 540

Query: 590 --GDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKD-SAHEDESTTQIKS 649
             G+EGI IDHL R++ KNVSAW MK+LMN+++ G LSTLDEQI+D +  ED+  TQI+S
Sbjct: 541 EGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRS 600

Query: 650 EYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKN 709
           E+EAK+AA++IFQNVA  GS+YIY+ED MRF+ EDE+ + M LFEGASE  KISKS LKN
Sbjct: 601 EFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKN 660

Query: 710 WVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQ 769
           WVVNAFRERRALALTLNDTKTAVN+LHR+V++LVS++IL+IWLLILGIAT+KFLL ++SQ
Sbjct: 661 WVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQ 720

Query: 770 LVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK 829
           L+LV FVFGN+CKT+FEA+IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK
Sbjct: 721 LLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK 780

Query: 830 IIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILSYIEGKKEHWCPA 889
           I++PNS+L TK I NYYRSPDM DA+EF +HI+TPPEK   +RQRILSY++ KK+HW P+
Sbjct: 781 IVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPS 840

Query: 890 PLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLN 939
           P+I+ +D+  LN ++IA+W TH+MNHQ+MGER+ RR  L+EE+ ++ +ELD++YRL PLN
Sbjct: 841 PMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLN 880

BLAST of CmoCh20G007290 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 909.1 bits (2348), Expect = 4.2e-263
Identity = 515/961 (53.59%), Postives = 685/961 (71.28%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60
           MDF   +FKSH SYK +R   G  S    E LPIL D  P++     S M V D      
Sbjct: 1   MDFR-NSFKSHSSYKQIRS-PGDQSEPSPEHLPILHDHHPDH-----SGMVVDDQ----- 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGE-- 120
              K D   +S +    A    ++S + W++++     D  + R + +       KG+  
Sbjct: 61  ---KPDSTRSSLDDGRNAPVERDASYKFWQDNTTGTSTDHTAVRTSDKDPIAISRKGDRL 120

Query: 121 --DFEFRQQRKDVEDPPSKLI-GQFLHKQ---KASGEMCLDMDMEMLELP-QDKTLPTVA 180
              F+F   +  V++ P+K++ G+ +++Q   + + E+ LD+D E  ++  Q    PT  
Sbjct: 121 SGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSHQTMPTPTST 180

Query: 181 ESPMRRSSKELKVSF----------------ESISEISESDSLRRRHRDSPVDEEHRVQH 240
                 +S+E++VSF                 S    S S +  R ++D P  +E     
Sbjct: 181 ARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEE---- 240

Query: 241 PPLRDHRPHGNGHDDGAAEVVSCTSNSSFERNVSFQRKSSLL-KTKTKSRLLDPP-EHQD 300
                             EVV CTS      N+SFQRKS L+ + KT+SRL DPP E + 
Sbjct: 241 ------------------EVVRCTS------NMSFQRKSELISRVKTRSRLQDPPREEET 300

Query: 301 RRSGRVPKSGQIRSGLLSKALDEEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAA 360
             SG   +SGQ++SGLL+    +E+DDP  EED+PDEYK+  L  +T+LQW SL+ IIAA
Sbjct: 301 PYSGW--RSGQLKSGLLADI--DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLVAIIAA 360

Query: 361 LICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLY 420
           L C+L+I+ W++ ++W L LWKWEV +LVLICGRL SGWGIRI+VFFIER+FLLRKRVLY
Sbjct: 361 LACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLY 420

Query: 421 FVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKT 480
           FVYGVR+AVQNCLWLGLVL+AW+FLFD KVQRE +S  L YVTK+L+C L+ST++WL+KT
Sbjct: 421 FVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLSTILWLIKT 480

Query: 481 LIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNA 540
           L+VKVLASSFHVSTYFDRIQ+AL+NQYVIETLSGPP+IE+ + +EEE +  +E+ K+QNA
Sbjct: 481 LVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNA 540

Query: 541 GATIPPDLRASAFSSPEGGRVIGSGGLQKSPRGRSAKLSRALSKK-GDEGITIDHLHRLS 600
           GA +PPDL A+AF   + GRV+            + KLS  + K   D GI+++HLHR++
Sbjct: 541 GANLPPDLCAAAFPPGKSGRVM------------NPKLSPIIPKSTTDNGISMEHLHRMN 600

Query: 601 PKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVAS 660
            KN+SAWNMKRLM IVR+ +L+TLDEQ+ +S +EDEST QI+SE EAK AA++IF+NV  
Sbjct: 601 HKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEAKAAARKIFKNVEQ 660

Query: 661 QGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLN 720
           +G+KYIYLEDLMRF+ EDEA KTM LFEGA E+++ISKS+LKNW+VNAFRERRALALTLN
Sbjct: 661 RGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRALALTLN 720

Query: 721 DTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFE 780
           DTKTAVNKLH M+NI+ +++I+VIWL++L IA+SK LLFV+SQ+VL+AF+FGNT KTVFE
Sbjct: 721 DTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVKTVFE 780

Query: 781 AIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYY 840
           +IIFLF++HP+DVGDRCEID VQ++VEEMNILTTVFLR+DN KI++PNS+L  K+I+NYY
Sbjct: 781 SIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSINNYY 840

Query: 841 RSPDMGDAVEFCLHISTPPEKIAIMRQRILSYIEGKKEHWCPAPLIILKDVEELNRMRIA 900
           RSPDMGDA+EFC+HI+TP EKI++++QRI +YI+ K E+W P   II+KD+E+L+ +R+A
Sbjct: 841 RSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHIVRLA 900

Query: 901 IWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPAT 934
           IW  HR+NHQDM ERWTRRA LVEE++KI  ELD+Q+R  PL+INV ++P V S+R+P  
Sbjct: 901 IWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVRTMPTVVSSRVPPG 902

BLAST of CmoCh20G007290 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 5.6e-231
Identity = 457/886 (51.58%), Postives = 610/886 (68.85%), Query Frame = 0

Query: 53  SDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNG 112
           ++ + +K+ +  +D+ D         +    SSG  +  +SY FW D          +  
Sbjct: 23  NEKTKKKKKLANLDDGD---------IAKTQSSGSSFDGNSYKFWQDI---------ATD 82

Query: 113 DGGKGEDFEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAE 172
           D  K   F+F Q R+                     E+ LD++ E  E         V+ 
Sbjct: 83  DYTKSGSFDFPQYRE---------------------EITLDVNEETEETED------VSN 142

Query: 173 SPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGA 232
           +     SKE +V F+  S  + + S   R   S            L          +D  
Sbjct: 143 NNNLSGSKETRVFFKINSSGTNNMSGSVRSCTSSTSFSSATMRLNLEQQL------EDEG 202

Query: 233 AEVVSCTSNSSFERNVSFQRKSSLL-KTKTKSRLLDPPEHQDRR-SGRVPKSGQIRSGLL 292
             VV C+S           RK+ L+ + K +SRL+DPP+ ++++ S  +  S Q+RSGLL
Sbjct: 203 EVVVRCSS----------VRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLL 262

Query: 293 SKALD--EEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKL 352
            +  D  EE+DD   EED+P EY+K  +  +T+LQW SLI ++ AL+ +L +  WR   L
Sbjct: 263 GRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATL 322

Query: 353 WKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWL 412
           W L LWKWEV++LVLICGRL SG GIRIIVFFIER+FLLRKRVLYFVYGV+ AVQNCLWL
Sbjct: 323 WSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWL 382

Query: 413 GLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTY 472
           GLVL+AW+FLFD KV++E +SD L  ++K+L+C L+ST++WL+KTL+VKVLASSFHVSTY
Sbjct: 383 GLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTY 442

Query: 473 FDRIQDALYNQYVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSS 532
           FDRIQ+AL++ Y+IETLSGPP++E+ + +EEE +  +E+ K+Q  GA + P+L ++AF  
Sbjct: 443 FDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQ 502

Query: 533 PEGGRVIGSGGLQKSPRGRSAKLSRALSKKG-DEGITIDHLHRLSPKNVSAWNMKRLMNI 592
            + G  +            + K S  + K G D GIT+D LH+++ KNVSAWNMKRLM I
Sbjct: 503 EKSGSTM------------NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKI 562

Query: 593 VRHGALSTLDEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFM 652
           VR+ +LSTLDEQ   +  EDEST QI+SE EAK AA++IF+NVA  G+K+IYLEDLMRF+
Sbjct: 563 VRNVSLSTLDEQALQNTCEDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFL 622

Query: 653 VEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 712
             DEA KTM LFEGA  ++KI+KS+LKNW+VNAFRERRALALTLNDTKTAVNKLH M++ 
Sbjct: 623 RVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISF 682

Query: 713 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGD 772
           L +++I+VIWL++L IATSK+LLF+TSQ+VL+AF+FGN+ KTVFE+IIFLF++HP+DVGD
Sbjct: 683 LTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGD 742

Query: 773 RCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHI 832
           R  ID V+M+VEEMNILTTVFLR DN KI++PN +L  KAIHNY RSPDMGD V  C+HI
Sbjct: 743 RLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHI 802

Query: 833 STPPEKIAIMRQRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGER 892
           +TPPEKIA ++QRI SYI+ K E+W P   +I+KDVE+LN +RIAIWL H++NHQ+MGER
Sbjct: 803 TTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGER 835

Query: 893 WTRRAFLVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATW 934
           +TRRA L+EE++KI  ELD+QYR  PL+INV ++P V S+R+P  W
Sbjct: 863 FTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSSRVPPAW 835

BLAST of CmoCh20G007290 vs. ExPASy TrEMBL
Match: A0A6J1FSP8 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111448441 PE=3 SV=1)

HSP 1 Score: 1798.5 bits (4657), Expect = 0.0e+00
Identity = 938/938 (100.00%), Postives = 938/938 (100.00%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD
Sbjct: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 939
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of CmoCh20G007290 vs. ExPASy TrEMBL
Match: A0A6J1J5E6 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111483636 PE=3 SV=1)

HSP 1 Score: 1757.3 bits (4550), Expect = 0.0e+00
Identity = 917/938 (97.76%), Postives = 923/938 (98.40%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTD+TQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDTTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120
           VIVKVD+AD SSNASAR VESVNSSGRIWRESSYDFWNDS  RRNNGEGS+GDGGKGEDF
Sbjct: 61  VIVKVDQADVSSNASARPVESVNSSGRIWRESSYDFWNDSGRRRNNGEGSDGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLP V ESP+RRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPPVVESPLRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQ PP RDHR HGNGHDDG  EVV CTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRAHGNGHDDGTPEVVRCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFER VSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSG+IRSGLLSKALDEEDDD
Sbjct: 241 NSSFERGVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGKIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVF IERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFLIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAK+IFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKKIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           ILSYIE KKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEDKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 939
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of CmoCh20G007290 vs. ExPASy TrEMBL
Match: A0A6J1CFM7 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011094 PE=3 SV=1)

HSP 1 Score: 1568.1 bits (4059), Expect = 0.0e+00
Identity = 824/945 (87.20%), Postives = 882/945 (93.33%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQD-QEQLPILSDQEPNNLHQKQSFMAVSDASDRK 60
           MDFSLK+FKSHGSYKY RKLSG     D QEQLPILSDQ+P NLHQ+QS MAVSDASDR 
Sbjct: 1   MDFSLKSFKSHGSYKYARKLSGGGGADDSQEQLPILSDQDPGNLHQQQS-MAVSDASDRN 60

Query: 61  EVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGED 120
           EVIVK+D+ D S NA++R +E+VN+SGRIWRESSYDFWND D RR+N EGSNGD G+ ED
Sbjct: 61  EVIVKIDQPD-SLNATSRVMEAVNASGRIWRESSYDFWNDGDGRRSNAEGSNGD-GRSED 120

Query: 121 FEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSS 180
           FEFRQ R+ VEDPPSKLIGQFLHKQKASGEM LDMDMEMLELPQDKTLP VAESPMRRSS
Sbjct: 121 FEFRQHRQGVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTLPPVAESPMRRSS 180

Query: 181 KELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCT 240
           KELKVSFESISEISE+DS+RRRHRDSP DEE++ Q PP RD R +GNGHDDG AEVV CT
Sbjct: 181 KELKVSFESISEISENDSMRRRHRDSPADEEYKGQQPPQRDRRAYGNGHDDGGAEVVRCT 240

Query: 241 SNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDR----RSGRV-PKSGQIRSGLLSKAL 300
           SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPE  DR    RSGRV PKSGQIRSGLLSKAL
Sbjct: 241 SNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGVRSGRVPPKSGQIRSGLLSKAL 300

Query: 301 DEEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELW 360
           DEEDDDPFLEEDLPDEYKK+NLG LT+LQWASLILIIAAL+CTL+I Y  RK LWKL+LW
Sbjct: 301 DEEDDDPFLEEDLPDEYKKSNLGALTLLQWASLILIIAALVCTLSIPYLNRKNLWKLKLW 360

Query: 361 KWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420
           KWEVM+LVLICGRL SGWGIRIIVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIA
Sbjct: 361 KWEVMVLVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420

Query: 421 WNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQD 480
           WNFLFDDKVQREVKS+ALEYVTKVL+CLLVSTLVWLVKTL+VKVLASSFHVSTYFDRIQD
Sbjct: 421 WNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQD 480

Query: 481 ALYNQYVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRV 540
           AL+NQYVIETLSGPPLIEI+KN+EEE +LAEEV+KLQNAGATIPPDL+A+AFS+ +GGRV
Sbjct: 481 ALFNQYVIETLSGPPLIEIRKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAKGGRV 540

Query: 541 IGSGGLQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALS 600
           IGSGGLQ+SPRGRS KLSR+LSK GD+GITIDHLH+LSPKNVSAWNMKRLMNIVRHGALS
Sbjct: 541 IGSGGLQRSPRGRSGKLSRSLSKNGDDGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS 600

Query: 601 TLDEQIKDSAH-EDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEAS 660
           TLDEQIKDS H EDES TQIKSE EAKVAAK+IFQNVA  GSKYIYLEDLMRFM EDEA 
Sbjct: 601 TLDEQIKDSTHEEDESATQIKSECEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEAL 660

Query: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVII 720
           KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+I
Sbjct: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI 720

Query: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780
           LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG
Sbjct: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780

Query: 781 VQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEK 840
           VQMIVEEMNILTT+FLR+DNQKI+FPNSVLATKAIHN+YRSPDMGDAVEFCLHISTPPEK
Sbjct: 781 VQMIVEEMNILTTIFLRYDNQKIVFPNSVLATKAIHNFYRSPDMGDAVEFCLHISTPPEK 840

Query: 841 IAIMRQRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAF 900
           IA+MRQRI+SYIE KKEHWCPAP+I+ KDVEELNR+RIAIWLTHRMNHQDMGERWTRRA 
Sbjct: 841 IAVMRQRIVSYIEAKKEHWCPAPMIVFKDVEELNRLRIAIWLTHRMNHQDMGERWTRRAL 900

Query: 901 LVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 939
           LVEEL+KIFQELDLQYRLLPL+INV +LPPVNSTRLPATW AT S
Sbjct: 901 LVEELLKIFQELDLQYRLLPLDINVRTLPPVNSTRLPATWTATTS 942

BLAST of CmoCh20G007290 vs. ExPASy TrEMBL
Match: A0A5D3DK11 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00230 PE=3 SV=1)

HSP 1 Score: 1563.1 bits (4046), Expect = 0.0e+00
Identity = 828/934 (88.65%), Postives = 872/934 (93.36%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60
           MDFSLK+FKSH SYKYVRKLSG D+TQDQE LPILSD      HQ  S MAVSDASDRKE
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDQEHLPILSD------HQ-HSIMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120
           VIVKVDEADAS   S RA + VN SGRIWRESSYDFWNDSD+RRNNGEG        EDF
Sbjct: 61  VIVKVDEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGEGG---ARTTEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKT-LPTVAESPMRRSS 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELPQDKT L TVAESPMRRSS
Sbjct: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSS 180

Query: 181 KELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHG-NGHDDGAAEVVSC 240
           +ELKVSFESISEISE+DS+RRRHRDSP+DEEHR Q P   D R HG NG DDGAAEV+ C
Sbjct: 181 RELKVSFESISEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRC 240

Query: 241 TSNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEED 300
           +SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPEHQDRRSGRVPKSGQ+RSGL+SK LDEED
Sbjct: 241 SSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEED 300

Query: 301 DDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEV 360
           DDPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTIRYWRRKKLWKLE+WKWEV
Sbjct: 301 DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEV 360

Query: 361 MILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420
           MILVLICGRL SGWGIR+IVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL
Sbjct: 361 MILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420

Query: 421 FDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYN 480
           FDDKVQREVKS+ALEYVTKVL+CLL+STLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+N
Sbjct: 421 FDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN 480

Query: 481 QYVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPE-GGRVIGS 540
           QYVIETLSGPPLIEIQKN+EEE +LAEEV+KLQNAGATIPPDL+A+AFS+ + GGRVIGS
Sbjct: 481 QYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIGS 540

Query: 541 GGLQKSPRGRSAKLSRALSKK-GDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTL 600
           GGLQKSPRGRS KLSR LSKK GDEGITIDHLH+LSPKNVSAWNMKRLMNIVRHG LSTL
Sbjct: 541 GGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTL 600

Query: 601 DEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTM 660
           DEQIKD+AHEDESTTQIKSEYEAKVAAK+IF NVA  GSKYIYLEDLMRFM +DEASKTM
Sbjct: 601 DEQIKDTAHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTM 660

Query: 661 SLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI 720
            LFEGA ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI
Sbjct: 661 GLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI 720

Query: 721 WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQM 780
           WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFE+IIFLFVMHPFDVGDRCEIDGVQM
Sbjct: 721 WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQM 780

Query: 781 IVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAI 840
           IVEEMNILTT+FLR+DNQKIIFPNSVLATKAIHN+YRSPDMGD VEFCLHISTPPEKIAI
Sbjct: 781 IVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIAI 840

Query: 841 MRQRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVE 900
           MRQRI+SYIEGKKEHWCPAP+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVE
Sbjct: 841 MRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVE 900

Query: 901 ELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLP 931
           ELVKIFQELDLQYRLLPL+INV SLPPVNST  P
Sbjct: 901 ELVKIFQELDLQYRLLPLDINVRSLPPVNSTNFP 921

BLAST of CmoCh20G007290 vs. ExPASy TrEMBL
Match: A0A1S3BIW1 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490566 PE=3 SV=1)

HSP 1 Score: 1563.1 bits (4046), Expect = 0.0e+00
Identity = 828/934 (88.65%), Postives = 872/934 (93.36%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60
           MDFSLK+FKSH SYKYVRKLSG D+TQDQE LPILSD      HQ  S MAVSDASDRKE
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDQEHLPILSD------HQ-HSIMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120
           VIVKVDEADAS   S RA + VN SGRIWRESSYDFWNDSD+RRNNGEG        EDF
Sbjct: 61  VIVKVDEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGEGG---ARTTEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKT-LPTVAESPMRRSS 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELPQDKT L TVAESPMRRSS
Sbjct: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSS 180

Query: 181 KELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHG-NGHDDGAAEVVSC 240
           +ELKVSFESISEISE+DS+RRRHRDSP+DEEHR Q P   D R HG NG DDGAAEV+ C
Sbjct: 181 RELKVSFESISEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRC 240

Query: 241 TSNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEED 300
           +SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPEHQDRRSGRVPKSGQ+RSGL+SK LDEED
Sbjct: 241 SSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEED 300

Query: 301 DDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEV 360
           DDPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTIRYWRRKKLWKLE+WKWEV
Sbjct: 301 DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEV 360

Query: 361 MILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420
           MILVLICGRL SGWGIR+IVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL
Sbjct: 361 MILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420

Query: 421 FDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYN 480
           FDDKVQREVKS+ALEYVTKVL+CLL+STLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+N
Sbjct: 421 FDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN 480

Query: 481 QYVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPE-GGRVIGS 540
           QYVIETLSGPPLIEIQKN+EEE +LAEEV+KLQNAGATIPPDL+A+AFS+ + GGRVIGS
Sbjct: 481 QYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIGS 540

Query: 541 GGLQKSPRGRSAKLSRALSKK-GDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTL 600
           GGLQKSPRGRS KLSR LSKK GDEGITIDHLH+LSPKNVSAWNMKRLMNIVRHG LSTL
Sbjct: 541 GGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTL 600

Query: 601 DEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTM 660
           DEQIKD+AHEDESTTQIKSEYEAKVAAK+IF NVA  GSKYIYLEDLMRFM +DEASKTM
Sbjct: 601 DEQIKDTAHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTM 660

Query: 661 SLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI 720
            LFEGA ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI
Sbjct: 661 GLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI 720

Query: 721 WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQM 780
           WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFE+IIFLFVMHPFDVGDRCEIDGVQM
Sbjct: 721 WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQM 780

Query: 781 IVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAI 840
           IVEEMNILTT+FLR+DNQKIIFPNSVLATKAIHN+YRSPDMGD VEFCLHISTPPEKIAI
Sbjct: 781 IVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIAI 840

Query: 841 MRQRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVE 900
           MRQRI+SYIEGKKEHWCPAP+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVE
Sbjct: 841 MRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVE 900

Query: 901 ELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLP 931
           ELVKIFQELDLQYRLLPL+INV SLPPVNST  P
Sbjct: 901 ELVKIFQELDLQYRLLPLDINVRSLPPVNSTNFP 921

BLAST of CmoCh20G007290 vs. NCBI nr
Match: XP_022943801.1 (mechanosensitive ion channel protein 6-like [Cucurbita moschata])

HSP 1 Score: 1798.5 bits (4657), Expect = 0.0e+00
Identity = 938/938 (100.00%), Postives = 938/938 (100.00%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD
Sbjct: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 939
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of CmoCh20G007290 vs. NCBI nr
Match: KAG6571002.1 (Mechanosensitive ion channel protein 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1787.7 bits (4629), Expect = 0.0e+00
Identity = 932/938 (99.36%), Postives = 935/938 (99.68%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPP RDHRPHGNGHDDGAAEVV CTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPQRDHRPHGNGHDDGAAEVVRCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD
Sbjct: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQK+KEEE KLAEEV+KLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAK+IFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKKIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 939
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of CmoCh20G007290 vs. NCBI nr
Match: XP_023512518.1 (mechanosensitive ion channel protein 6-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512519.1 mechanosensitive ion channel protein 6-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1778.1 bits (4604), Expect = 0.0e+00
Identity = 927/938 (98.83%), Postives = 932/938 (99.36%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQK+SFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDS+RNNGEGSNGDGGKGEDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELP DKTLPTVAESPMRRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQ PP RDHRPHGNGHDDGAAEVV CTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD
Sbjct: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQK+KEEE KLAEEV+KLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           IL +IEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 939
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of CmoCh20G007290 vs. NCBI nr
Match: XP_022985632.1 (mechanosensitive ion channel protein 6-like [Cucurbita maxima])

HSP 1 Score: 1757.3 bits (4550), Expect = 0.0e+00
Identity = 917/938 (97.76%), Postives = 923/938 (98.40%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTD+TQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDTTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120
           VIVKVD+AD SSNASAR VESVNSSGRIWRESSYDFWNDS  RRNNGEGS+GDGGKGEDF
Sbjct: 61  VIVKVDQADVSSNASARPVESVNSSGRIWRESSYDFWNDSGRRRNNGEGSDGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLP V ESP+RRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPPVVESPLRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQ PP RDHR HGNGHDDG  EVV CTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRAHGNGHDDGTPEVVRCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFER VSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSG+IRSGLLSKALDEEDDD
Sbjct: 241 NSSFERGVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGKIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVF IERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFLIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAK+IFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKKIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           ILSYIE KKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEDKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 939
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of CmoCh20G007290 vs. NCBI nr
Match: XP_022140409.1 (mechanosensitive ion channel protein 6-like [Momordica charantia])

HSP 1 Score: 1568.1 bits (4059), Expect = 0.0e+00
Identity = 824/945 (87.20%), Postives = 882/945 (93.33%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQD-QEQLPILSDQEPNNLHQKQSFMAVSDASDRK 60
           MDFSLK+FKSHGSYKY RKLSG     D QEQLPILSDQ+P NLHQ+QS MAVSDASDR 
Sbjct: 1   MDFSLKSFKSHGSYKYARKLSGGGGADDSQEQLPILSDQDPGNLHQQQS-MAVSDASDRN 60

Query: 61  EVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGED 120
           EVIVK+D+ D S NA++R +E+VN+SGRIWRESSYDFWND D RR+N EGSNGD G+ ED
Sbjct: 61  EVIVKIDQPD-SLNATSRVMEAVNASGRIWRESSYDFWNDGDGRRSNAEGSNGD-GRSED 120

Query: 121 FEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSS 180
           FEFRQ R+ VEDPPSKLIGQFLHKQKASGEM LDMDMEMLELPQDKTLP VAESPMRRSS
Sbjct: 121 FEFRQHRQGVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTLPPVAESPMRRSS 180

Query: 181 KELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCT 240
           KELKVSFESISEISE+DS+RRRHRDSP DEE++ Q PP RD R +GNGHDDG AEVV CT
Sbjct: 181 KELKVSFESISEISENDSMRRRHRDSPADEEYKGQQPPQRDRRAYGNGHDDGGAEVVRCT 240

Query: 241 SNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDR----RSGRV-PKSGQIRSGLLSKAL 300
           SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPE  DR    RSGRV PKSGQIRSGLLSKAL
Sbjct: 241 SNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGVRSGRVPPKSGQIRSGLLSKAL 300

Query: 301 DEEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELW 360
           DEEDDDPFLEEDLPDEYKK+NLG LT+LQWASLILIIAAL+CTL+I Y  RK LWKL+LW
Sbjct: 301 DEEDDDPFLEEDLPDEYKKSNLGALTLLQWASLILIIAALVCTLSIPYLNRKNLWKLKLW 360

Query: 361 KWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420
           KWEVM+LVLICGRL SGWGIRIIVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIA
Sbjct: 361 KWEVMVLVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420

Query: 421 WNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQD 480
           WNFLFDDKVQREVKS+ALEYVTKVL+CLLVSTLVWLVKTL+VKVLASSFHVSTYFDRIQD
Sbjct: 421 WNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQD 480

Query: 481 ALYNQYVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRV 540
           AL+NQYVIETLSGPPLIEI+KN+EEE +LAEEV+KLQNAGATIPPDL+A+AFS+ +GGRV
Sbjct: 481 ALFNQYVIETLSGPPLIEIRKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAKGGRV 540

Query: 541 IGSGGLQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALS 600
           IGSGGLQ+SPRGRS KLSR+LSK GD+GITIDHLH+LSPKNVSAWNMKRLMNIVRHGALS
Sbjct: 541 IGSGGLQRSPRGRSGKLSRSLSKNGDDGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS 600

Query: 601 TLDEQIKDSAH-EDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEAS 660
           TLDEQIKDS H EDES TQIKSE EAKVAAK+IFQNVA  GSKYIYLEDLMRFM EDEA 
Sbjct: 601 TLDEQIKDSTHEEDESATQIKSECEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEAL 660

Query: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVII 720
           KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+I
Sbjct: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI 720

Query: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780
           LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG
Sbjct: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780

Query: 781 VQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEK 840
           VQMIVEEMNILTT+FLR+DNQKI+FPNSVLATKAIHN+YRSPDMGDAVEFCLHISTPPEK
Sbjct: 781 VQMIVEEMNILTTIFLRYDNQKIVFPNSVLATKAIHNFYRSPDMGDAVEFCLHISTPPEK 840

Query: 841 IAIMRQRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAF 900
           IA+MRQRI+SYIE KKEHWCPAP+I+ KDVEELNR+RIAIWLTHRMNHQDMGERWTRRA 
Sbjct: 841 IAVMRQRIVSYIEAKKEHWCPAPMIVFKDVEELNRLRIAIWLTHRMNHQDMGERWTRRAL 900

Query: 901 LVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 939
           LVEEL+KIFQELDLQYRLLPL+INV +LPPVNSTRLPATW AT S
Sbjct: 901 LVEELLKIFQELDLQYRLLPLDINVRTLPPVNSTRLPATWTATTS 942

BLAST of CmoCh20G007290 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 969.9 bits (2506), Expect = 1.4e-282
Identity = 545/923 (59.05%), Postives = 676/923 (73.24%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGE- 109
           MAV DA+DR+EVIVK+D  + ++N       S  + G+IWR+ SYDFW D +   N G  
Sbjct: 1   MAV-DAADRREVIVKIDGENGNNNG-----VSGETVGKIWRDGSYDFWTDGEGNLNKGHN 60

Query: 110 ----------GSNGDGGKGEDFEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEM 169
                      + G+  K E FEFR+     EDPP+KLIGQFLHKQ+ASGE+CLDMD+ M
Sbjct: 61  AAAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGM 120

Query: 170 LELPQDKTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPL 229
            EL Q + L  V+ESP R S+K                         PV           
Sbjct: 121 DEL-QSRGLTPVSESP-RVSTKR-----------------------DPVGR--------- 180

Query: 230 RDHRPHGNGHDDGAAEVVSCTSNSSFERNVSFQRKSS-LLKTKTKSRLLDPPEHQ----- 289
           RD R + N +DDG  EVV C+ N     N   QR SS LLK +T+SRL DPP  Q     
Sbjct: 181 RDSRSNTNNNDDG--EVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQT 240

Query: 290 -DRRSGRVPKSGQIRSGLLSKAL----DEEDDDPFLEEDLPDEYKKANLGVLTMLQWASL 349
            D +SGR+PKSGQ++SG   K+     +EE+DDPF  EDLP+EY+K  L +  +L+W SL
Sbjct: 241 ADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSL 300

Query: 350 ILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLL 409
           ILIIA  +CTL I   R+KKLW+L+LWKWE M+LVLICGRL S W ++I+VFFIER+FLL
Sbjct: 301 ILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLL 360

Query: 410 RKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTL 469
           RKRVLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV +   + AL  VTK+ +CLLV  L
Sbjct: 361 RKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFL 420

Query: 470 VWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKNKEEEVKLAEEV 529
           +WLVKTL+VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPPLIEIQKN+EEE +++ EV
Sbjct: 421 LWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEV 480

Query: 530 IKLQNAGATIPPDLRASAFSSPEGGRVIGSGGLQKSP--RGRSAKLSRALSKKG-----D 589
            K QN G                   V    G QKSP   G+S  LS  LS  G     +
Sbjct: 481 KKFQNPGG------------------VEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGEN 540

Query: 590 EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSEYEAK 649
           +GITID LH+L+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D + +D+   QI+SE+EAK
Sbjct: 541 KGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAK 600

Query: 650 VAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNWVVNA 709
           +AA++IF NVA  GSK+IY  D+MRF+ +DEA KT+SLFEGASE+ +ISKSSLKNWVVNA
Sbjct: 601 LAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNA 660

Query: 710 FRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVA 769
           FRERRALALTLNDTKTAVN+LH+MVNI+V +IILVIWL+ILGI ++KFL+ ++SQ+V+VA
Sbjct: 661 FRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVA 720

Query: 770 FVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPN 829
           F+FGN CK VFE+II+LFV+HPFDVGDRCEIDGVQM+VEEMNILTTVFLRFDNQK+++PN
Sbjct: 721 FIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPN 780

Query: 830 SVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILSYIEGKKEHWCPAPLIIL 889
           S+L TK+I NYYRSPDMGD +EF +HI+TP EKI +++QRI SYIEGKK+HW PAP+I+ 
Sbjct: 781 SLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVF 840

Query: 890 KDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNINVCS 939
           KD+E LN +RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +ELD++YRL PL+INV +
Sbjct: 841 KDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRN 853

BLAST of CmoCh20G007290 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 921.8 bits (2381), Expect = 4.5e-268
Identity = 510/921 (55.37%), Postives = 665/921 (72.20%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEG 109
           MA  D++DR++ IV ++    S    A    S    G IW+ESSYDFW D +  +N+ +G
Sbjct: 1   MAAVDSTDRRDFIVNIN-GQESGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKG 60

Query: 110 SNGDGGKGEDFEFRQQRK------DVEDPPSKLIGQFLHKQKASG-EMCLDMDMEMLELP 169
            + D   G  F FRQ+ +      ++ DPPSKLIGQFLHKQ+ASG E+ LD+++ M EL 
Sbjct: 61  DDED-EDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQ 120

Query: 170 QD-KTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPV----DEEHRVQHPP 229
            +    P  A +  RR    +  S   +    ++D++RRR   + +    DEE       
Sbjct: 121 SNTPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEE------- 180

Query: 230 LRDHRPHGNGHDDGAAEVVSCTSNSSFERNVSFQRKSSLLKTKTKSRLLDPP-------E 289
                    G +   AEV+ C S           +K  L + KTKSRL DPP       +
Sbjct: 181 ---------GRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAID 240

Query: 290 HQDRRSGRVPKSGQIRSGLLSKA-----------LDEEDDDPFLEEDLPDEYKKANLGVL 349
             + +SGR  +SG  +SG L K+            +EE++DPFL+EDLP+E+K+  L   
Sbjct: 241 KTEMKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFW 300

Query: 350 TMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVF 409
             L+W SL+LI+ +L+C+LTI   +RK  WKL+LWKWEV +LVLICGRL S W +RIIVF
Sbjct: 301 VFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVF 360

Query: 410 FIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVL 469
            +E++F  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VL
Sbjct: 361 LVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVL 420

Query: 470 ICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKNKEE 529
           +CLLV+ ++WLVKT++VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPPL+EIQ+ +EE
Sbjct: 421 VCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEE 480

Query: 530 EVKLAEEVIKLQN-AGATIPPDLRASAFSSPEGGRVIGSGGLQKSPRGRSAKLSRALSKK 589
           E ++AE+V  L+  AGA +PP L+A+  S  +               G+S  L+R  SK+
Sbjct: 481 EQQVAEDVKSLEKLAGAKLPPALKATVKSFMK--------------VGKSPGLNRIGSKR 540

Query: 590 GD--EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSE 649
           G+  EGI ID L R++ KNVSAWNMKRLMNI+  GA+STLD+ ++D+  EDE  T I+SE
Sbjct: 541 GEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSE 600

Query: 650 YEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNW 709
           YEAK AA++IF NV   GS+YIYLED +RF+ E+EA + M+LFEGASES KISKS LKNW
Sbjct: 601 YEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNW 660

Query: 710 VVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQL 769
           VV AFRERRALALTLNDTKTAV++LHR++N+++ +II++IWLLILGIAT++FLL ++SQL
Sbjct: 661 VVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQL 720

Query: 770 VLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKI 829
           +LVAFVFGN+CKT+FEAIIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTTVFLR+DNQKI
Sbjct: 721 LLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKI 780

Query: 830 IFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILSYIEGKKEHWCPAP 889
           I+PNSVL TK I NYYRSPDMGDAVEFC+HI+TPPEKI  ++QRILSY++ KK++W PAP
Sbjct: 781 IYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAP 840

Query: 890 LIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNI 934
           +I+   +++LN ++IA+WLTHRMNHQDMGER+ RR  L+EE+ K  +ELD++YRL PLNI
Sbjct: 841 MIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNI 875

BLAST of CmoCh20G007290 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 912.9 bits (2358), Expect = 2.1e-265
Identity = 522/926 (56.37%), Postives = 659/926 (71.17%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFW-NDSDSRRNNGE 109
           MAV     R++ +V++D  D               S + WRESS +FW ND  S+   GE
Sbjct: 1   MAVDSTDQRRDFVVRIDGEDN------------GDSEKFWRESSINFWHNDKSSKPPGGE 60

Query: 110 GSNGDGGKGEDFEFRQQRKDVE-DPPSKLIGQFLHKQKASG-EMCLDMDMEMLELPQDKT 169
             +G      DF  R   K  E DPPSKLI QFL+KQKASG E+ LDM+  M EL Q  T
Sbjct: 61  EDDG----SFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPEL-QKNT 120

Query: 170 LPTVAESPMRRSSKELKVSFESISEISESDSLRRRHR-----------DSPVDEEHRVQH 229
           +P ++ + +  S+  +     +       D++RRR             DS  DEE+RV  
Sbjct: 121 VPPLSSTAVSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRV-- 180

Query: 230 PPLRDHRPHGNGHDDGAAEVVSCTSNSSFERNVSFQRKSSLLKTKTKSRLLDP--PEHQD 289
                         DG +EVV CTSN       S  R  +L+K KT+SRL+DP  P + D
Sbjct: 181 --------------DG-SEVVKCTSNR------STMRTKTLMKMKTRSRLMDPPTPTYPD 240

Query: 290 RRSGRVPKSGQIRSGLL-------------SKALDEEDDDPFLEEDLPDEYKKANLGVLT 349
             SGR P+SG +  G               SK L EE++DPF EEDLP+  +K  + V  
Sbjct: 241 MVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDL-EEEEDPFSEEDLPEGLRKEKICVWV 300

Query: 350 MLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFF 409
           +++W  LILIIA+LIC+L I Y R K LW L LWKWEVM+LVLICGRL S W +++ V+F
Sbjct: 301 IIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYF 360

Query: 410 IERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLI 469
           +E +FL RK+VLYFVYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTKVLI
Sbjct: 361 VESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLI 420

Query: 470 CLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKNKEEE 529
           CLLV+ ++WL+KTL+VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPP IEI     EE
Sbjct: 421 CLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIH---IEE 480

Query: 530 VKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGLQKSPR--GRSAKLSRALSKK 589
            K+A +V   +  G  + P L   A SSP     +GSG LQKSP   G+S  LSR+ SKK
Sbjct: 481 EKVANDVKTFEIVGRKLSP-LGPKAVSSPP-QVTVGSGRLQKSPSRVGKSPVLSRSGSKK 540

Query: 590 --GDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKD-SAHEDESTTQIKS 649
             G+EGI IDHL R++ KNVSAW MK+LMN+++ G LSTLDEQI+D +  ED+  TQI+S
Sbjct: 541 EGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRS 600

Query: 650 EYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKN 709
           E+EAK+AA++IFQNVA  GS+YIY+ED MRF+ EDE+ + M LFEGASE  KISKS LKN
Sbjct: 601 EFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKN 660

Query: 710 WVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQ 769
           WVVNAFRERRALALTLNDTKTAVN+LHR+V++LVS++IL+IWLLILGIAT+KFLL ++SQ
Sbjct: 661 WVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQ 720

Query: 770 LVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK 829
           L+LV FVFGN+CKT+FEA+IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK
Sbjct: 721 LLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK 780

Query: 830 IIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILSYIEGKKEHWCPA 889
           I++PNS+L TK I NYYRSPDM DA+EF +HI+TPPEK   +RQRILSY++ KK+HW P+
Sbjct: 781 IVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPS 840

Query: 890 PLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLN 939
           P+I+ +D+  LN ++IA+W TH+MNHQ+MGER+ RR  L+EE+ ++ +ELD++YRL PLN
Sbjct: 841 PMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLN 880

BLAST of CmoCh20G007290 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 851.7 bits (2199), Expect = 5.7e-247
Identity = 485/921 (52.66%), Postives = 632/921 (68.62%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEG 109
           MA  D++DR++ IV ++    S    A    S    G IW+ESSYDFW D +  +N+ +G
Sbjct: 1   MAAVDSTDRRDFIVNIN-GQESGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKG 60

Query: 110 SNGDGGKGEDFEFRQQRK------DVEDPPSKLIGQFLHKQKASG-EMCLDMDMEMLELP 169
            + D   G  F FRQ+ +      ++ DPPSKLIGQFLHKQ+ASG E+ LD+++ M EL 
Sbjct: 61  DDED-EDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQ 120

Query: 170 QD-KTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPV----DEEHRVQHPP 229
            +    P  A +  RR    +  S   +    ++D++RRR   + +    DEE       
Sbjct: 121 SNTPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEE------- 180

Query: 230 LRDHRPHGNGHDDGAAEVVSCTSNSSFERNVSFQRKSSLLKTKTKSRLLDPP-------E 289
                    G +   AEV+ C S           +K  L + KTKSRL DPP       +
Sbjct: 181 ---------GRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAID 240

Query: 290 HQDRRSGRVPKSGQIRSGLLSKA-----------LDEEDDDPFLEEDLPDEYKKANLGVL 349
             + +SGR  +SG  +SG L K+            +EE++DPFL+EDLP+E+K+  L   
Sbjct: 241 KTEMKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFW 300

Query: 350 TMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVF 409
             L+W SL+LI+ +L+C+LTI   +RK  WKL+LWKWEV +LVLICGRL S W +RIIVF
Sbjct: 301 VFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVF 360

Query: 410 FIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVL 469
            +E++F  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL       
Sbjct: 361 LVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALR------ 420

Query: 470 ICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKNKEE 529
                                      TYFDRIQ++L+ QYVIETLSGPPL+EIQ+ +EE
Sbjct: 421 ---------------------------TYFDRIQESLFTQYVIETLSGPPLMEIQRMEEE 480

Query: 530 EVKLAEEVIKLQN-AGATIPPDLRASAFSSPEGGRVIGSGGLQKSPRGRSAKLSRALSKK 589
           E ++AE+V  L+  AGA +PP L+A+  S  +               G+S  L+R  SK+
Sbjct: 481 EQQVAEDVKSLEKLAGAKLPPALKATVKSFMK--------------VGKSPGLNRIGSKR 540

Query: 590 GD--EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSE 649
           G+  EGI ID L R++ KNVSAWNMKRLMNI+  GA+STLD+ ++D+  EDE  T I+SE
Sbjct: 541 GEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSE 600

Query: 650 YEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNW 709
           YEAK AA++IF NV   GS+YIYLED +RF+ E+EA + M+LFEGASES KISKS LKNW
Sbjct: 601 YEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNW 660

Query: 710 VVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQL 769
           V  AFRERRALALTLNDTKTAV++LHR++N+++ +II++IWLLILGIAT++FLL ++SQL
Sbjct: 661 V--AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQL 720

Query: 770 VLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKI 829
           +LVAFVFGN+CKT+FEAIIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTTVFLR+DNQKI
Sbjct: 721 LLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKI 780

Query: 830 IFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILSYIEGKKEHWCPAP 889
           I+PNSVL TK I NYYRSPDMGDAVEFC+HI+TPPEKI  ++QRILSY++ KK++W PAP
Sbjct: 781 IYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAP 840

Query: 890 LIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNI 934
           +I+   +++LN ++IA+WLTHRMNHQDMGER+ RR  L+EE+ K  +ELD++YRL PLNI
Sbjct: 841 MIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNI 840

BLAST of CmoCh20G007290 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 802.4 bits (2071), Expect = 4.0e-232
Identity = 457/886 (51.58%), Postives = 610/886 (68.85%), Query Frame = 0

Query: 53  SDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNG 112
           ++ + +K+ +  +D+ D         +    SSG  +  +SY FW D          +  
Sbjct: 23  NEKTKKKKKLANLDDGD---------IAKTQSSGSSFDGNSYKFWQDI---------ATD 82

Query: 113 DGGKGEDFEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAE 172
           D  K   F+F Q R+                     E+ LD++ E  E         V+ 
Sbjct: 83  DYTKSGSFDFPQYRE---------------------EITLDVNEETEETED------VSN 142

Query: 173 SPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGA 232
           +     SKE +V F+  S  + + S   R   S            L          +D  
Sbjct: 143 NNNLSGSKETRVFFKINSSGTNNMSGSVRSCTSSTSFSSATMRLNLEQQL------EDEG 202

Query: 233 AEVVSCTSNSSFERNVSFQRKSSLL-KTKTKSRLLDPPEHQDRR-SGRVPKSGQIRSGLL 292
             VV C+S           RK+ L+ + K +SRL+DPP+ ++++ S  +  S Q+RSGLL
Sbjct: 203 EVVVRCSS----------VRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLL 262

Query: 293 SKALD--EEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKL 352
            +  D  EE+DD   EED+P EY+K  +  +T+LQW SLI ++ AL+ +L +  WR   L
Sbjct: 263 GRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATL 322

Query: 353 WKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWL 412
           W L LWKWEV++LVLICGRL SG GIRIIVFFIER+FLLRKRVLYFVYGV+ AVQNCLWL
Sbjct: 323 WSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWL 382

Query: 413 GLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTY 472
           GLVL+AW+FLFD KV++E +SD L  ++K+L+C L+ST++WL+KTL+VKVLASSFHVSTY
Sbjct: 383 GLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTY 442

Query: 473 FDRIQDALYNQYVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSS 532
           FDRIQ+AL++ Y+IETLSGPP++E+ + +EEE +  +E+ K+Q  GA + P+L ++AF  
Sbjct: 443 FDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQ 502

Query: 533 PEGGRVIGSGGLQKSPRGRSAKLSRALSKKG-DEGITIDHLHRLSPKNVSAWNMKRLMNI 592
            + G  +            + K S  + K G D GIT+D LH+++ KNVSAWNMKRLM I
Sbjct: 503 EKSGSTM------------NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKI 562

Query: 593 VRHGALSTLDEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFM 652
           VR+ +LSTLDEQ   +  EDEST QI+SE EAK AA++IF+NVA  G+K+IYLEDLMRF+
Sbjct: 563 VRNVSLSTLDEQALQNTCEDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFL 622

Query: 653 VEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 712
             DEA KTM LFEGA  ++KI+KS+LKNW+VNAFRERRALALTLNDTKTAVNKLH M++ 
Sbjct: 623 RVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISF 682

Query: 713 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGD 772
           L +++I+VIWL++L IATSK+LLF+TSQ+VL+AF+FGN+ KTVFE+IIFLF++HP+DVGD
Sbjct: 683 LTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGD 742

Query: 773 RCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHI 832
           R  ID V+M+VEEMNILTTVFLR DN KI++PN +L  KAIHNY RSPDMGD V  C+HI
Sbjct: 743 RLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHI 802

Query: 833 STPPEKIAIMRQRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGER 892
           +TPPEKIA ++QRI SYI+ K E+W P   +I+KDVE+LN +RIAIWL H++NHQ+MGER
Sbjct: 803 TTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGER 835

Query: 893 WTRRAFLVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATW 934
           +TRRA L+EE++KI  ELD+QYR  PL+INV ++P V S+R+P  W
Sbjct: 863 FTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSSRVPPAW 835