CmoCh20G006470 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G006470
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein NRT1/ PTR FAMILY 6.4-like
LocationCmo_Chr20: 3168011 .. 3171065 (-)
RNA-Seq ExpressionCmoCh20G006470
SyntenyCmoCh20G006470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTTGCCTTCCAATAAATCATGAGTCTAAGCACAACTGCAGCACACAAACAATTCATCTTCTTTCTCTTTCTCTCTCTCTCTCTTTCTCTCTAGCAGAGAGGAAAAAACAAGCAAAAAACAATGGTAACTTGTTTGTGGAGAAACTCCTTAGTTCCTATTGGCTATGAATGGCTTCTTTAACGGTTAAAATCTTCCTTGTTTCCAACAGGTTCTGGTATCTAAGCATGGGAGTGGAGGAGGAGAAGAAGATGCTGCAGTGGATTTTTGGGGAAACCCAGTGGACAAATCAAAAACTGGAGGATGGCTTGCTGCAGGGCTCATCTTAGGTGCTTAATTTTTTCTTTAGTTTTCACAGACCAAGAGTCTAAATCTATCAATTCTAATGCCCAAAATGATGCAGGAACTGAGCTCTCTGAAAGGATATGTGTGATGGGGATATCAATGAACTTAGTGACATATTTAGTTGGGGATTTGCATCTCTCTTCAGCTAAATCTGCAACTATTGTGACTAATTTTCTTGGTGCCCTTAATCTTCTTGGCCTTCTTGGTGGGTTCTTAGCAGATGCCAAGTTGGGACGTTATCTAACTGTTGCAACTTTTGCTTCCATAACTGCTGTGGTAAGTTGTGAGCCATTAAGAATGATTTCAGCAAAGAAATTTCTAGTTCAGAATTTAGCCTAAGCCTTGCTGGTTTTTTGCAGGGTGTTATTTTGTTGACATTGGCCACAACCATCCCTGGCATGCGACCCCCTCATTGTGATGACTCCAGAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTTGCCATGCTCTATGCAGCACTTTATACTATAGCACTCGGCGGAGGGGGGATAAAATCAAACGTTTCAGGATTTGGGTCCGACCAATTCGATGTCAATGATCCTAAGGAGGAGAAGGCCATGATATTCTTCTTCAATAGGTTCTACTTTGCCATAAGCATTGGATCCTTGTTTGCTGTCATTGTGTTGGTTTATGTACAAGACAATGTTGGGAGAGGCTGGGGATATGGAATCTCTGGAGGGACCATGGTGATTGCTGTGATTGTACTGCTTTGTGGGACAACCTTTTACAGGTTTAAGAAGCCTAGAGGAAGTCCTCTAACAGTGATATGGAGGGTGGCTCTTTTGGCTTGGAAGAAGAGAAGTCTCCCTCTCCCATCTCATCCCAGCTTCTTAAATGACTACCAAAATGCCAAGGTTCCTTACACCTACAGGTTCAAGTAAGTGTTTTTCAGATCTATCATCAAATACACTTCGTTTTTTTTCCCCCTCTTTATTGTAAAGAAAACGGTGTTAATGAAAAGGTGAATATCAGGTGTCTTGACAAGGCTGCAATTCTGGACGAGTATGTTGCCAACAATCGAAACAGAAACGATCCATGGATTGTCTCGACCGTGACTGAAGTTGAAGAAGTGAAAATGGTGCTGAAGCTTTTACCTATTTGGTCTACTGGCATCCTCTTTTGGACGATCTACTCTCAAATGACTACGTTCACGGTAGAGCAAGCATCATTTATGGATAGAAAAGTTGGTAGTTTTGAGATTCCTCCTGGTTCTATGTCAGCCTTTCTATTCATCACCATCCTTCTCGTCACTTCCTTGAACGAGAAACTATTCATACCAATTGCACGAAAGCTGACTCACAATGCCCAAGGTCTCACAAGCCTTCAGAGGATTGGAATTGGACTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGAGTTGTTGAGAAAGAGAGGAGGACATTAGCTTTGGGACAACGTACTCTAAGTGCTTTCTGGTTAATACCTCAGTTCTTCTTGATTGGTGCTGGAGAGGCATTTACATATGTTGGACAACTCGAGTTTTTTATCAGAGAAGCACCGGAACGAATGAAATCCATGAGCACAGGGCTTTTTCTTAGCACTCTTTCCATGGGATTTTTCGTTAGTAGCTTGTTGGTAACAATCGTGGATAAAGTAACCAACAAGCAATGGCTGAGAAGTAATTTGAACATGGGAAACTTGAACTACTTCTATTGGCTACTTGCAGTGTTGGGGCTGCTAAACTTTTTCTTCTTTCTAGTCCTAGCCCGGAAACATCAGTATAAAGACCAGCATTACTTCAACTCTAAAGACAACAATGAGGAGCTCAAGAGTTCTAATGACTTGGTAGCACTTGAAGTGGAAGGGCCAATAAGCATAAAGGCAACAGAAAGTTAAGAGTGTGTGTTTCAAGGTGTCCCATGTATAAGTTTAAAAATCCTGCATCTACGTTTGAATATCTCAAAACCTGTAGCTACAACCATCCTATGCTTTCAGCATCAATGGCAGATAAGCCACTGCTATTTTGTCTTCCCCTAGGTTCTATATGATCTAAACCATTCAGCATATTTCAAATAATGTTTTCCAGTGCAAACCGGCACTCATTTATGTAAACATCCATGTCTCATAGCAGGCTTCTTACTGGAAGTAGAATCCTCAAATGATTTTGATAGGCATCATTTTCAAACAGGCATCTATTCCAATAAATTATTTTAGCTAGCAACTAGACTCAGCAAGATGTATAGAGTATAAAAGCCTTGATTATCAAATAATTCCAGAAAAGTACACTGCTCAAATATAATCCTTCTCACATCGTCCCGATAAATGAGAAGTAGACCTGATATCTAACAGCAATGCATGTTTCAAGAGGCAGAAGCAGAACTTGGAGAAGGAAGGTGATTTACAACTAATGGAGGTCGGAGGGCCGCATGCTGTTCCACCTTCTGTTTACGAGCTTTATACATTTCTTCTGTTTCAACCACTACTAATCGCTTTACCTATAATAAAATTAAGCAAAACAACGAAAAAGGGATCATGGCATTCAGTCACCATGAGGTTGTTCTGTAGTGTGTGCATGTGCGCAGTAGAGAATAAGTATAAAAAGCAACCTGTTGAAGCATTCCCCTAACAGTTGGTGTGTTCCAGCGCATGACAGTTCGGTTGCACTTTCGGAGCCGCAATGCCTCTAATGTACGTCGGTGAAGCCTCCTTGTTCCTGGC

mRNA sequence

CTTTTTGCCTTCCAATAAATCATGAGTCTAAGCACAACTGCAGCACACAAACAATTCATCTTCTTTCTCTTTCTCTCTCTCTCTCTTTCTCTCTAGCAGAGAGGAAAAAACAAGCAAAAAACAATGGTTCTGGTATCTAAGCATGGGAGTGGAGGAGGAGAAGAAGATGCTGCAGTGGATTTTTGGGGAAACCCAGTGGACAAATCAAAAACTGGAGGATGGCTTGCTGCAGGGCTCATCTTAGGAACTGAGCTCTCTGAAAGGATATGTGTGATGGGGATATCAATGAACTTAGTGACATATTTAGTTGGGGATTTGCATCTCTCTTCAGCTAAATCTGCAACTATTGTGACTAATTTTCTTGGTGCCCTTAATCTTCTTGGCCTTCTTGGTGGGTTCTTAGCAGATGCCAAGTTGGGACGTTATCTAACTGTTGCAACTTTTGCTTCCATAACTGCTGTGGGTGTTATTTTGTTGACATTGGCCACAACCATCCCTGGCATGCGACCCCCTCATTGTGATGACTCCAGAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTTGCCATGCTCTATGCAGCACTTTATACTATAGCACTCGGCGGAGGGGGGATAAAATCAAACGTTTCAGGATTTGGGTCCGACCAATTCGATGTCAATGATCCTAAGGAGGAGAAGGCCATGATATTCTTCTTCAATAGGTTCTACTTTGCCATAAGCATTGGATCCTTGTTTGCTGTCATTGTGTTGGTTTATGTACAAGACAATGTTGGGAGAGGCTGGGGATATGGAATCTCTGGAGGGACCATGGTGATTGCTGTGATTGTACTGCTTTGTGGGACAACCTTTTACAGGTTTAAGAAGCCTAGAGGAAGTCCTCTAACAGTGATATGGAGGGTGGCTCTTTTGGCTTGGAAGAAGAGAAGTCTCCCTCTCCCATCTCATCCCAGCTTCTTAAATGACTACCAAAATGCCAAGGTTCCTTACACCTACAGGTTCAAGTGTCTTGACAAGGCTGCAATTCTGGACGAGTATGTTGCCAACAATCGAAACAGAAACGATCCATGGATTGTCTCGACCGTGACTGAAGTTGAAGAAGTGAAAATGGTGCTGAAGCTTTTACCTATTTGGTCTACTGGCATCCTCTTTTGGACGATCTACTCTCAAATGACTACGTTCACGGTAGAGCAAGCATCATTTATGGATAGAAAAGTTGGTAGTTTTGAGATTCCTCCTGGTTCTATGTCAGCCTTTCTATTCATCACCATCCTTCTCGTCACTTCCTTGAACGAGAAACTATTCATACCAATTGCACGAAAGCTGACTCACAATGCCCAAGGTCTCACAAGCCTTCAGAGGATTGGAATTGGACTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGAGTTGTTGAGAAAGAGAGGAGGACATTAGCTTTGGGACAACGTACTCTAAGTGCTTTCTGGTTAATACCTCAGTTCTTCTTGATTGGTGCTGGAGAGGCATTTACATATGTTGGACAACTCGAGTTTTTTATCAGAGAAGCACCGGAACGAATGAAATCCATGAGCACAGGGCTTTTTCTTAGCACTCTTTCCATGGGATTTTTCGTTAGTAGCTTGTTGGTAACAATCGTGGATAAAGTAACCAACAAGCAATGGCTGAGAAGTAATTTGAACATGGGAAACTTGAACTACTTCTATTGGCTACTTGCAGTGTTGGGGCTGCTAAACTTTTTCTTCTTTCTAGTCCTAGCCCGGAAACATCAGTATAAAGACCAGCATTACTTCAACTCTAAAGACAACAATGAGGAGCTCAAGAGTTCTAATGACTTGGTAGCACTTGAAGTGGAAGGGCCAATAAGCATAAAGGCAACAGAAAGTTAAGAGTGTGTGTTTCAAGGTGTCCCATGTATAAGTTTAAAAATCCTGCATCTACGTTTGAATATCTCAAAACCTGTAGCTACAACCATCCTATGCTTTCAGCATCAATGGCAGATAAGCCACTGCTATTTTGTCTTCCCCTAGGTTCTATATGATCTAAACCATTCAGCATATTTCAAATAATGTTTTCCAGTGCAAACCGGCACTCATTTATGTAAACATCCATGTCTCATAGCAGGCTTCTTACTGGAAGTAGAATCCTCAAATGATTTTGATAGGCATCATTTTCAAACAGGCATCTATTCCAATAAATTATTTTAGCTAGCAACTAGACTCAGCAAGATGTATAGAGTATAAAAGCCTTGATTATCAAATAATTCCAGAAAAGTACACTGCTCAAATATAATCCTTCTCACATCGTCCCGATAAATGAGAAGTAGACCTGATATCTAACAGCAATGCATGTTTCAAGAGGCAGAAGCAGAACTTGGAGAAGGAAGGTGATTTACAACTAATGGAGGTCGGAGGGCCGCATGCTGTTCCACCTTCTGTTTACGAGCTTTATACATTTCTTCTGTTTCAACCACTACTAATCGCTTTACCTATAATAAAATTAAGCAAAACAACGAAAAAGGGATCATGGCATTCAGTCACCATGAGGTTGTTCTGTAGTGTGTGCATGTGCGCAGTAGAGAATAAGTATAAAAAGCAACCTGTTGAAGCATTCCCCTAACAGTTGGTGTGTTCCAGCGCATGACAGTTCGGTTGCACTTTCGGAGCCGCAATGCCTCTAATGTACGTCGGTGAAGCCTCCTTGTTCCTGGC

Coding sequence (CDS)

ATGGTTCTGGTATCTAAGCATGGGAGTGGAGGAGGAGAAGAAGATGCTGCAGTGGATTTTTGGGGAAACCCAGTGGACAAATCAAAAACTGGAGGATGGCTTGCTGCAGGGCTCATCTTAGGAACTGAGCTCTCTGAAAGGATATGTGTGATGGGGATATCAATGAACTTAGTGACATATTTAGTTGGGGATTTGCATCTCTCTTCAGCTAAATCTGCAACTATTGTGACTAATTTTCTTGGTGCCCTTAATCTTCTTGGCCTTCTTGGTGGGTTCTTAGCAGATGCCAAGTTGGGACGTTATCTAACTGTTGCAACTTTTGCTTCCATAACTGCTGTGGGTGTTATTTTGTTGACATTGGCCACAACCATCCCTGGCATGCGACCCCCTCATTGTGATGACTCCAGAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTTGCCATGCTCTATGCAGCACTTTATACTATAGCACTCGGCGGAGGGGGGATAAAATCAAACGTTTCAGGATTTGGGTCCGACCAATTCGATGTCAATGATCCTAAGGAGGAGAAGGCCATGATATTCTTCTTCAATAGGTTCTACTTTGCCATAAGCATTGGATCCTTGTTTGCTGTCATTGTGTTGGTTTATGTACAAGACAATGTTGGGAGAGGCTGGGGATATGGAATCTCTGGAGGGACCATGGTGATTGCTGTGATTGTACTGCTTTGTGGGACAACCTTTTACAGGTTTAAGAAGCCTAGAGGAAGTCCTCTAACAGTGATATGGAGGGTGGCTCTTTTGGCTTGGAAGAAGAGAAGTCTCCCTCTCCCATCTCATCCCAGCTTCTTAAATGACTACCAAAATGCCAAGGTTCCTTACACCTACAGGTTCAAGTGTCTTGACAAGGCTGCAATTCTGGACGAGTATGTTGCCAACAATCGAAACAGAAACGATCCATGGATTGTCTCGACCGTGACTGAAGTTGAAGAAGTGAAAATGGTGCTGAAGCTTTTACCTATTTGGTCTACTGGCATCCTCTTTTGGACGATCTACTCTCAAATGACTACGTTCACGGTAGAGCAAGCATCATTTATGGATAGAAAAGTTGGTAGTTTTGAGATTCCTCCTGGTTCTATGTCAGCCTTTCTATTCATCACCATCCTTCTCGTCACTTCCTTGAACGAGAAACTATTCATACCAATTGCACGAAAGCTGACTCACAATGCCCAAGGTCTCACAAGCCTTCAGAGGATTGGAATTGGACTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGAGTTGTTGAGAAAGAGAGGAGGACATTAGCTTTGGGACAACGTACTCTAAGTGCTTTCTGGTTAATACCTCAGTTCTTCTTGATTGGTGCTGGAGAGGCATTTACATATGTTGGACAACTCGAGTTTTTTATCAGAGAAGCACCGGAACGAATGAAATCCATGAGCACAGGGCTTTTTCTTAGCACTCTTTCCATGGGATTTTTCGTTAGTAGCTTGTTGGTAACAATCGTGGATAAAGTAACCAACAAGCAATGGCTGAGAAGTAATTTGAACATGGGAAACTTGAACTACTTCTATTGGCTACTTGCAGTGTTGGGGCTGCTAAACTTTTTCTTCTTTCTAGTCCTAGCCCGGAAACATCAGTATAAAGACCAGCATTACTTCAACTCTAAAGACAACAATGAGGAGCTCAAGAGTTCTAATGACTTGGTAGCACTTGAAGTGGAAGGGCCAATAAGCATAAAGGCAACAGAAAGTTAA

Protein sequence

MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDKAAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLNFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Homology
BLAST of CmoCh20G006470 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 3.4e-234
Identity = 412/554 (74.37%), Postives = 465/554 (83.94%), Query Frame = 0

Query: 5   SKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGD 64
           S HG+  G E+ A D+ GNP DKSKTGGWL AGLILG+ELSERICVMGISMNLVTYLVGD
Sbjct: 6   SSHGAKDGSEE-AYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGD 65

Query: 65  LHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTI 124
           LH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGRY  VA  AS+TA+GV+LLT+ATTI
Sbjct: 66  LHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTI 125

Query: 125 PGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDP 184
             MRPP CDD RR  HQCIEANG QLA+LY ALYTIALGGGGIKSNVSGFGSDQFD +DP
Sbjct: 126 SSMRPPICDDFRRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDP 185

Query: 185 KEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTT 244
           KEEK MIFFFNRFYF+IS+GSLFAVI LVYVQDNVGRGWGYGIS  TMV+A IVLLCGT 
Sbjct: 186 KEEKQMIFFFNRFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTK 245

Query: 245 FYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDKAAI- 304
            YRFKKP+GSP T IWRV  LAWKKR    P+HPS LN Y N  VP+T   KCLDKAAI 
Sbjct: 246 RYRFKKPKGSPFTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDKAAIS 305

Query: 305 LDEYVANNRN--RNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQAS 364
            +E   ++++    DPWIVSTVT+VEEVK+V+KL+PIW+T ILFWTIYSQMTTFTVEQA+
Sbjct: 306 KNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQAT 365

Query: 365 FMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLV 424
           FMDRK+GSF +P GS SAFL +TILL TSLNE++F+P+ R+LT   QG+TSLQRIG+GLV
Sbjct: 366 FMDRKLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLV 425

Query: 425 FSIFGMVAAGVVEKERRTLAL-GQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 484
           FS+  M  A V+E  RR  A+   + +SAFWL+PQ+FL+GAGEAF YVGQLEFFIREAPE
Sbjct: 426 FSMAAMAVAAVIENARREAAVNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPE 485

Query: 485 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 544
           RMKSMSTGLFLST+SMGFFVSSLLV++VD+VT+K WLRSNLN   LNYFYWLL VLG LN
Sbjct: 486 RMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVLGALN 545

Query: 545 FFFFLVLARKHQYK 555
           F  F+V A KHQYK
Sbjct: 546 FLIFIVFAMKHQYK 558

BLAST of CmoCh20G006470 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 6.1e-151
Identity = 284/559 (50.81%), Postives = 379/559 (67.80%), Query Frame = 0

Query: 17  AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLSSAKSATIV 76
           A DF G P D+SKTGGW +A +IL  E  ER+  +GI +NLVTYL G +HL +A +A  V
Sbjct: 15  AWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANTV 74

Query: 77  TNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDDSR 136
           TNFLG   +L LLGGF+AD  LGRYLT+A FA+I A GV +LTL+T IPG+RPP C+ + 
Sbjct: 75  TNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPTT 134

Query: 137 RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFFFNR 196
                C +A+G QL +LY ALY  ALG GG+K++VSGFGSDQFD  +PKE   M +FFNR
Sbjct: 135 SS--HCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNR 194

Query: 197 FYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPL 256
           F+F I++GSL AV VLVYVQD+VGR WGYGI    +V+A+ V L GT  YRFKK  GSP+
Sbjct: 195 FFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPM 254

Query: 257 TVIWRVALLAWKKRSLPLPSHPSFLNDYQN-----------AKVPYTYRFKCLDKAAILD 316
           T +  V + AW+ R L LP+ PS+L D  +            K+P+T +F+ LDKAAI D
Sbjct: 255 TQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRD 314

Query: 317 EYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDR 376
           +      N  + W +ST+T+VEEVK ++++LPIW+T ILFWT+++Q+TT +V Q+  +DR
Sbjct: 315 QEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDR 374

Query: 377 KVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSIF 436
            +GSFEIPP SM+ F    +LL T++ +++ I + +KL +   GL  LQRIG+GL F   
Sbjct: 375 SIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSM 434

Query: 437 GMVAAGVVEKERRTLA------LGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAP 496
            M  A +VE +R   A      +    L  + LIPQ+ ++G GEA  Y GQL+FF+RE P
Sbjct: 435 AMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECP 494

Query: 497 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK--QWLRSNLNMGNLNYFYWLLAVLG 556
           + MK MSTGL LSTL++GFF SS+LVTIV+K T K   W+  +LN G L  FYWL+AVL 
Sbjct: 495 KGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLV 554

BLAST of CmoCh20G006470 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.9e-147
Identity = 282/546 (51.65%), Postives = 383/546 (70.15%), Query Frame = 0

Query: 17  AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLSSAKSATIV 76
           AVD+ G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTYL+  +HL S+ SA IV
Sbjct: 12  AVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIV 71

Query: 77  TNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDDSR 136
           T+F+G   LL LLGGFLAD+ LGR+ T+  F++I A+G   L +AT +P +RPP C    
Sbjct: 72  TDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTCHHG- 131

Query: 137 RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFFFNR 196
                CI A   Q+ +LY +LY IALG GG+KS++SGFGSDQFD  DPKE+  M FFFNR
Sbjct: 132 ---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNR 191

Query: 197 FYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPL 256
           F+F IS+G+L AV VLVY+QD VGR W YGI   +M IA+++ LCGT  YR+KK +GSP+
Sbjct: 192 FFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPV 251

Query: 257 TVIWRVALLAWKKRSLPLPSHPSFL--NDYQNAKVPYTYRFKCLDKAAILD----EYVAN 316
             I++V   A++KR + LP    +L  ++ +  ++ +T +F  LDKAAI+     E   +
Sbjct: 252 VQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLD 311

Query: 317 NRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDRKVGSF 376
                +PW +S+VT+VEEVKM+++LLPIW+T I+FWT Y+QM TF+VEQAS M R +GSF
Sbjct: 312 GVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSF 371

Query: 377 EIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSIFGMVAA 436
           +IP GS++ F    IL+  ++ ++  +P  +K      G +SLQRI IGLV S  GM AA
Sbjct: 372 KIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIGLVLSTAGMAAA 431

Query: 437 GVVEKERRTLA--LGQRTL--SAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPERMKSMS 496
            +VE++R ++A    Q+TL  S F L+PQFFL+GAGEAF Y GQL+FFI ++P+ MK+MS
Sbjct: 432 ALVEQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMS 491

Query: 497 TGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----WLRSNLNMGNLNYFYWLLAVLGLLNFF 549
           TGLFL+TLS+GFFVSS LV+IV +VT+      WL  N+N G L+YFYWLL +L  +NF 
Sbjct: 492 TGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFV 551

BLAST of CmoCh20G006470 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.9e-124
Identity = 246/561 (43.85%), Postives = 353/561 (62.92%), Query Frame = 0

Query: 14  EDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLSSAKSA 73
           +D  VD   NP +K KTG W A   ILG E  ER+   G+  NLV YL   L+  +A +A
Sbjct: 9   QDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAA 68

Query: 74  TIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCD 133
             VTN+ G   +  L+G F+ADA LGRY T+ATF  I   G+ LLTL+ ++PG++P +C+
Sbjct: 69  NNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN 128

Query: 134 DSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFF 193
                       N  Q A+ + ALY IALG GGIK  VS FG+DQFD ND  E+     F
Sbjct: 129 ------ADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSF 188

Query: 194 FNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRG 253
           FN FYF+I++G+L A  VLV++Q NVG GWG+G+    MVIAV     G+ FYR ++P G
Sbjct: 189 FNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGG 248

Query: 254 SPLTVIWRVALLAWKKRSLPLPSHPSFL--------NDYQNAKVPYTYRFKCLDKAAILD 313
           SPLT I++V + A++K S+ +P   S L        N   + K+ +T   K  DKAA+  
Sbjct: 249 SPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVES 308

Query: 314 EYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDR 373
           +  +      +PW + +VT+VEE+K ++ LLP+W+TGI+F T+YSQM+T  V Q + MD+
Sbjct: 309 QSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQ 368

Query: 374 KVG-SFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSI 433
            +G +FEIP  S+S F  +++L  T + ++  IP+ARK T N +G T LQR+GIGLV SI
Sbjct: 369 HMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSI 428

Query: 434 FGMVAAGVVE-------KERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIRE 493
           F M+ AGV+E       K        Q  +S FW IPQ+ LIG  E FT++GQLEFF  +
Sbjct: 429 FAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQ 488

Query: 494 APERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----WLRSNLNMGNLNYFYWLL 553
           AP+ M+S+ + L L+T+++G ++S++LVT+V K+T K     W+  NLN G+L+YF++LL
Sbjct: 489 APDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLL 548

Query: 554 AVLGLLNFFFFLVLARKHQYK 555
           A L  LNF  +L ++++++YK
Sbjct: 549 ATLSFLNFLVYLWISKRYKYK 563

BLAST of CmoCh20G006470 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.7e-116
Identity = 233/561 (41.53%), Postives = 344/561 (61.32%), Query Frame = 0

Query: 14  EDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLSSAKSA 73
           +D  +D    P +K+KTG W A   ILGTE  ER+   G+S NL+ YL   +++ +  ++
Sbjct: 10  KDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSAS 69

Query: 74  TIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCD 133
             V+N+ G      L+G F+ADA LGRY T+A+F  I   G+ LLT++ ++PG+ P  C 
Sbjct: 70  KSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT-CS 129

Query: 134 DSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFF 193
                  +   A  GQ A+ + ALY IALG GGIK  VS FG+DQFD  D KE+++   F
Sbjct: 130 G------ETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSF 189

Query: 194 FNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRG 253
           FN FYF I++G++ A  VLV++Q NVG GWG G+    M IAV+    G+ FYR +KP G
Sbjct: 190 FNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGG 249

Query: 254 SPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNA--------KVPYTYRFKCLDKAAILD 313
           SPLT + +V + + +K  + +P   S L + Q+A        K+ +T      DKAA+  
Sbjct: 250 SPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVET 309

Query: 314 EYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDR 373
           E       ++  W + TVT+VEE+K +++LLPIW+TGI+F ++YSQM T  V Q + +D+
Sbjct: 310 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 369

Query: 374 KVG-SFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSI 433
            +G +F+IP  S+S F  +++L    + +KL +P ARK T + +G T LQRIGIGLV SI
Sbjct: 370 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 429

Query: 434 FGMVAAGVVEKERRTLALGQR-------TLSAFWLIPQFFLIGAGEAFTYVGQLEFFIRE 493
           F MV+AG++E  R                ++ FW +PQ+FL+G  E FT++GQLEFF  +
Sbjct: 430 FSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQ 489

Query: 494 APERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK----QWLRSNLNMGNLNYFYWLL 553
           AP+ M+S+ + L L+ ++ G ++S+ LVT+V KVT       W+  NLN G+L+YF+WLL
Sbjct: 490 APDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLL 549

Query: 554 AVLGLLNFFFFLVLARKHQYK 555
           A L  LNF  +L +A+ + YK
Sbjct: 550 AGLSFLNFLVYLWIAKWYTYK 563

BLAST of CmoCh20G006470 vs. ExPASy TrEMBL
Match: A0A6J1FWL4 (protein NRT1/ PTR FAMILY 6.4-like OS=Cucurbita moschata OX=3662 GN=LOC111448847 PE=3 SV=1)

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL
Sbjct: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480
           VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE
Sbjct: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480

Query: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 540
           RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN
Sbjct: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 540

Query: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of CmoCh20G006470 vs. ExPASy TrEMBL
Match: A0A6J1JG19 (protein NRT1/ PTR FAMILY 6.4-like OS=Cucurbita maxima OX=3661 GN=LOC111484070 PE=3 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 584/590 (98.98%), Postives = 586/590 (99.32%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVALLA KKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVALLASKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYVAN++NRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYVANDQNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFMDRKVGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL
Sbjct: 361 SFMDRKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480
           VFSIFGMVAAGVVEKERRTLALGQR LSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE
Sbjct: 421 VFSIFGMVAAGVVEKERRTLALGQRNLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480

Query: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 540
           RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLN GNLNYFYWLLAVLGLLN
Sbjct: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGNLNYFYWLLAVLGLLN 540

Query: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of CmoCh20G006470 vs. ExPASy TrEMBL
Match: A0A5D3CCF7 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold757G00100 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.3e-309
Identity = 542/591 (91.71%), Postives = 567/591 (95.94%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGGE+DAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDS RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
            NDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRV LLAWKKRSLP P+HPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAI+DEY     + N+PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAIIDEYATT--DPNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFM+R+VGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHN QGLTSLQRIGIGL
Sbjct: 361 SFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRT-LSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAP 480
           VFSIFGMVAAG VEKER+  A+GQ T +SAFWLIPQFFL+GAGEAF YVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WLRSNLN G+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           NFFFFL+LARKHQYKDQHY +SKDNNEELK SND+VALE+EGPIS+KA E+
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAET 589

BLAST of CmoCh20G006470 vs. ExPASy TrEMBL
Match: A0A1S3C263 (protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo OX=3656 GN=LOC103495995 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.3e-309
Identity = 542/591 (91.71%), Postives = 567/591 (95.94%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGGE+DAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDS RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
            NDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRV LLAWKKRSLP P+HPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAI+DEY     + N+PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAIIDEYATT--DPNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFM+R+VGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHN QGLTSLQRIGIGL
Sbjct: 361 SFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRT-LSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAP 480
           VFSIFGMVAAG VEKER+  A+GQ T +SAFWLIPQFFL+GAGEAF YVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WLRSNLN G+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           NFFFFL+LARKHQYKDQHY +SKDNNEELK SND+VALE+EGPIS+KA E+
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAET 589

BLAST of CmoCh20G006470 vs. ExPASy TrEMBL
Match: A0A5A7V3P8 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2406G00170 PE=3 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.7e-308
Identity = 541/591 (91.54%), Postives = 567/591 (95.94%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGGE+DAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVG+LHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGELHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDS RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
            NDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRV LLAWKKRSLP P+HPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAI+DEY     + N+PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAIIDEYATT--DPNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFM+R+VGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHN QGLTSLQRIGIGL
Sbjct: 361 SFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRT-LSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAP 480
           VFSIFGMVAAG VEKER+  A+GQ T +SAFWLIPQFFL+GAGEAF YVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WLRSNLN G+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           NFFFFL+LARKHQYKDQHY +SKDNNEELK SND+VALE+EGPIS+KA E+
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISMKAAET 589

BLAST of CmoCh20G006470 vs. NCBI nr
Match: XP_022944384.1 (protein NRT1/ PTR FAMILY 6.4-like [Cucurbita moschata])

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL
Sbjct: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480
           VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE
Sbjct: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480

Query: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 540
           RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN
Sbjct: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 540

Query: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of CmoCh20G006470 vs. NCBI nr
Match: KAG6570905.1 (Protein NRT1/ PTR FAMILY 6.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 588/590 (99.66%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVY+QDNVGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYIQDNVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYVAN+RNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL
Sbjct: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480
           VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE
Sbjct: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480

Query: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 540
           RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN
Sbjct: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 540

Query: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of CmoCh20G006470 vs. NCBI nr
Match: XP_023512951.1 (protein NRT1/ PTR FAMILY 6.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 585/590 (99.15%), Postives = 586/590 (99.32%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSG GEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGEGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYVAN+RNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYVANDRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFM RKVGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL
Sbjct: 361 SFMGRKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480
           VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE
Sbjct: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480

Query: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 540
           RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLN GNLNYFYWLLAVLGLLN
Sbjct: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGNLNYFYWLLAVLGLLN 540

Query: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of CmoCh20G006470 vs. NCBI nr
Match: XP_022986273.1 (protein NRT1/ PTR FAMILY 6.4-like [Cucurbita maxima])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 584/590 (98.98%), Postives = 586/590 (99.32%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVALLA KKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVALLASKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYVAN++NRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYVANDQNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFMDRKVGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL
Sbjct: 361 SFMDRKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480
           VFSIFGMVAAGVVEKERRTLALGQR LSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE
Sbjct: 421 VFSIFGMVAAGVVEKERRTLALGQRNLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 480

Query: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 540
           RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLN GNLNYFYWLLAVLGLLN
Sbjct: 481 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGNLNYFYWLLAVLGLLN 540

Query: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES
Sbjct: 541 FFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 590

BLAST of CmoCh20G006470 vs. NCBI nr
Match: XP_008455932.1 (PREDICTED: protein NRT1/ PTR FAMILY 6.4 [Cucumis melo] >TYK09533.1 protein NRT1/ PTR FAMILY 6.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.8e-309
Identity = 542/591 (91.71%), Postives = 567/591 (95.94%), Query Frame = 0

Query: 1   MVLVSKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGGE+DAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDS RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 VNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
            NDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRV LLAWKKRSLP P+HPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAI+DEY     + N+PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAIIDEYATT--DPNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGL 420
           SFM+R+VGSFEIPPGSMSAFLFI ILLVTSLNEKLFIPIARKLTHN QGLTSLQRIGIGL
Sbjct: 361 SFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 VFSIFGMVAAGVVEKERRTLALGQRT-LSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAP 480
           VFSIFGMVAAG VEKER+  A+GQ T +SAFWLIPQFFL+GAGEAF YVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WLRSNLN G+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLVLARKHQYKDQHYFNSKDNNEELKSSNDLVALEVEGPISIKATES 591
           NFFFFL+LARKHQYKDQHY +SKDNNEELK SND+VALE+EGPIS+KA E+
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAET 589

BLAST of CmoCh20G006470 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 812.4 bits (2097), Expect = 2.4e-235
Identity = 412/554 (74.37%), Postives = 465/554 (83.94%), Query Frame = 0

Query: 5   SKHGSGGGEEDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGD 64
           S HG+  G E+ A D+ GNP DKSKTGGWL AGLILG+ELSERICVMGISMNLVTYLVGD
Sbjct: 6   SSHGAKDGSEE-AYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGD 65

Query: 65  LHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTI 124
           LH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGRY  VA  AS+TA+GV+LLT+ATTI
Sbjct: 66  LHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTI 125

Query: 125 PGMRPPHCDDSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDP 184
             MRPP CDD RR  HQCIEANG QLA+LY ALYTIALGGGGIKSNVSGFGSDQFD +DP
Sbjct: 126 SSMRPPICDDFRRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDP 185

Query: 185 KEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTT 244
           KEEK MIFFFNRFYF+IS+GSLFAVI LVYVQDNVGRGWGYGIS  TMV+A IVLLCGT 
Sbjct: 186 KEEKQMIFFFNRFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTK 245

Query: 245 FYRFKKPRGSPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNAKVPYTYRFKCLDKAAI- 304
            YRFKKP+GSP T IWRV  LAWKKR    P+HPS LN Y N  VP+T   KCLDKAAI 
Sbjct: 246 RYRFKKPKGSPFTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDKAAIS 305

Query: 305 LDEYVANNRN--RNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQAS 364
            +E   ++++    DPWIVSTVT+VEEVK+V+KL+PIW+T ILFWTIYSQMTTFTVEQA+
Sbjct: 306 KNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQAT 365

Query: 365 FMDRKVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLV 424
           FMDRK+GSF +P GS SAFL +TILL TSLNE++F+P+ R+LT   QG+TSLQRIG+GLV
Sbjct: 366 FMDRKLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLV 425

Query: 425 FSIFGMVAAGVVEKERRTLAL-GQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPE 484
           FS+  M  A V+E  RR  A+   + +SAFWL+PQ+FL+GAGEAF YVGQLEFFIREAPE
Sbjct: 426 FSMAAMAVAAVIENARREAAVNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPE 485

Query: 485 RMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNMGNLNYFYWLLAVLGLLN 544
           RMKSMSTGLFLST+SMGFFVSSLLV++VD+VT+K WLRSNLN   LNYFYWLL VLG LN
Sbjct: 486 RMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVLGALN 545

Query: 545 FFFFLVLARKHQYK 555
           F  F+V A KHQYK
Sbjct: 546 FLIFIVFAMKHQYK 558

BLAST of CmoCh20G006470 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 535.8 bits (1379), Expect = 4.4e-152
Identity = 284/559 (50.81%), Postives = 379/559 (67.80%), Query Frame = 0

Query: 17  AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLSSAKSATIV 76
           A DF G P D+SKTGGW +A +IL  E  ER+  +GI +NLVTYL G +HL +A +A  V
Sbjct: 15  AWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANTV 74

Query: 77  TNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDDSR 136
           TNFLG   +L LLGGF+AD  LGRYLT+A FA+I A GV +LTL+T IPG+RPP C+ + 
Sbjct: 75  TNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPTT 134

Query: 137 RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFFFNR 196
                C +A+G QL +LY ALY  ALG GG+K++VSGFGSDQFD  +PKE   M +FFNR
Sbjct: 135 SS--HCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNR 194

Query: 197 FYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPL 256
           F+F I++GSL AV VLVYVQD+VGR WGYGI    +V+A+ V L GT  YRFKK  GSP+
Sbjct: 195 FFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPM 254

Query: 257 TVIWRVALLAWKKRSLPLPSHPSFLNDYQN-----------AKVPYTYRFKCLDKAAILD 316
           T +  V + AW+ R L LP+ PS+L D  +            K+P+T +F+ LDKAAI D
Sbjct: 255 TQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRD 314

Query: 317 EYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDR 376
           +      N  + W +ST+T+VEEVK ++++LPIW+T ILFWT+++Q+TT +V Q+  +DR
Sbjct: 315 QEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDR 374

Query: 377 KVGSFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSIF 436
            +GSFEIPP SM+ F    +LL T++ +++ I + +KL +   GL  LQRIG+GL F   
Sbjct: 375 SIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSM 434

Query: 437 GMVAAGVVEKERRTLA------LGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIREAP 496
            M  A +VE +R   A      +    L  + LIPQ+ ++G GEA  Y GQL+FF+RE P
Sbjct: 435 AMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECP 494

Query: 497 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK--QWLRSNLNMGNLNYFYWLLAVLG 556
           + MK MSTGL LSTL++GFF SS+LVTIV+K T K   W+  +LN G L  FYWL+AVL 
Sbjct: 495 KGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLV 554

BLAST of CmoCh20G006470 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 524.2 bits (1349), Expect = 1.3e-148
Identity = 282/546 (51.65%), Postives = 383/546 (70.15%), Query Frame = 0

Query: 17  AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLSSAKSATIV 76
           AVD+ G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTYL+  +HL S+ SA IV
Sbjct: 12  AVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIV 71

Query: 77  TNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDDSR 136
           T+F+G   LL LLGGFLAD+ LGR+ T+  F++I A+G   L +AT +P +RPP C    
Sbjct: 72  TDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTCHHG- 131

Query: 137 RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFFFNR 196
                CI A   Q+ +LY +LY IALG GG+KS++SGFGSDQFD  DPKE+  M FFFNR
Sbjct: 132 ---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNR 191

Query: 197 FYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPL 256
           F+F IS+G+L AV VLVY+QD VGR W YGI   +M IA+++ LCGT  YR+KK +GSP+
Sbjct: 192 FFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPV 251

Query: 257 TVIWRVALLAWKKRSLPLPSHPSFL--NDYQNAKVPYTYRFKCLDKAAILD----EYVAN 316
             I++V   A++KR + LP    +L  ++ +  ++ +T +F  LDKAAI+     E   +
Sbjct: 252 VQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLD 311

Query: 317 NRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDRKVGSF 376
                +PW +S+VT+VEEVKM+++LLPIW+T I+FWT Y+QM TF+VEQAS M R +GSF
Sbjct: 312 GVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSF 371

Query: 377 EIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSIFGMVAA 436
           +IP GS++ F    IL+  ++ ++  +P  +K      G +SLQRI IGLV S  GM AA
Sbjct: 372 KIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIGLVLSTAGMAAA 431

Query: 437 GVVEKERRTLA--LGQRTL--SAFWLIPQFFLIGAGEAFTYVGQLEFFIREAPERMKSMS 496
            +VE++R ++A    Q+TL  S F L+PQFFL+GAGEAF Y GQL+FFI ++P+ MK+MS
Sbjct: 432 ALVEQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMS 491

Query: 497 TGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----WLRSNLNMGNLNYFYWLLAVLGLLNFF 549
           TGLFL+TLS+GFFVSS LV+IV +VT+      WL  N+N G L+YFYWLL +L  +NF 
Sbjct: 492 TGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFV 551

BLAST of CmoCh20G006470 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 447.2 bits (1149), Expect = 2.0e-125
Identity = 246/561 (43.85%), Postives = 353/561 (62.92%), Query Frame = 0

Query: 14  EDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLSSAKSA 73
           +D  VD   NP +K KTG W A   ILG E  ER+   G+  NLV YL   L+  +A +A
Sbjct: 9   QDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAA 68

Query: 74  TIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCD 133
             VTN+ G   +  L+G F+ADA LGRY T+ATF  I   G+ LLTL+ ++PG++P +C+
Sbjct: 69  NNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN 128

Query: 134 DSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFF 193
                       N  Q A+ + ALY IALG GGIK  VS FG+DQFD ND  E+     F
Sbjct: 129 ------ADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSF 188

Query: 194 FNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRG 253
           FN FYF+I++G+L A  VLV++Q NVG GWG+G+    MVIAV     G+ FYR ++P G
Sbjct: 189 FNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGG 248

Query: 254 SPLTVIWRVALLAWKKRSLPLPSHPSFL--------NDYQNAKVPYTYRFKCLDKAAILD 313
           SPLT I++V + A++K S+ +P   S L        N   + K+ +T   K  DKAA+  
Sbjct: 249 SPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVES 308

Query: 314 EYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDR 373
           +  +      +PW + +VT+VEE+K ++ LLP+W+TGI+F T+YSQM+T  V Q + MD+
Sbjct: 309 QSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQ 368

Query: 374 KVG-SFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSI 433
            +G +FEIP  S+S F  +++L  T + ++  IP+ARK T N +G T LQR+GIGLV SI
Sbjct: 369 HMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSI 428

Query: 434 FGMVAAGVVE-------KERRTLALGQRTLSAFWLIPQFFLIGAGEAFTYVGQLEFFIRE 493
           F M+ AGV+E       K        Q  +S FW IPQ+ LIG  E FT++GQLEFF  +
Sbjct: 429 FAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQ 488

Query: 494 APERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----WLRSNLNMGNLNYFYWLL 553
           AP+ M+S+ + L L+T+++G ++S++LVT+V K+T K     W+  NLN G+L+YF++LL
Sbjct: 489 APDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLL 548

Query: 554 AVLGLLNFFFFLVLARKHQYK 555
           A L  LNF  +L ++++++YK
Sbjct: 549 ATLSFLNFLVYLWISKRYKYK 563

BLAST of CmoCh20G006470 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 421.4 bits (1082), Expect = 1.2e-117
Identity = 233/561 (41.53%), Postives = 344/561 (61.32%), Query Frame = 0

Query: 14  EDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLSSAKSA 73
           +D  +D    P +K+KTG W A   ILGTE  ER+   G+S NL+ YL   +++ +  ++
Sbjct: 10  KDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSAS 69

Query: 74  TIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCD 133
             V+N+ G      L+G F+ADA LGRY T+A+F  I   G+ LLT++ ++PG+ P  C 
Sbjct: 70  KSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT-CS 129

Query: 134 DSRRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDVNDPKEEKAMIFF 193
                  +   A  GQ A+ + ALY IALG GGIK  VS FG+DQFD  D KE+++   F
Sbjct: 130 G------ETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSF 189

Query: 194 FNRFYFAISIGSLFAVIVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRG 253
           FN FYF I++G++ A  VLV++Q NVG GWG G+    M IAV+    G+ FYR +KP G
Sbjct: 190 FNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGG 249

Query: 254 SPLTVIWRVALLAWKKRSLPLPSHPSFLNDYQNA--------KVPYTYRFKCLDKAAILD 313
           SPLT + +V + + +K  + +P   S L + Q+A        K+ +T      DKAA+  
Sbjct: 250 SPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVET 309

Query: 314 EYVANNRNRNDPWIVSTVTEVEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMDR 373
           E       ++  W + TVT+VEE+K +++LLPIW+TGI+F ++YSQM T  V Q + +D+
Sbjct: 310 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 369

Query: 374 KVG-SFEIPPGSMSAFLFITILLVTSLNEKLFIPIARKLTHNAQGLTSLQRIGIGLVFSI 433
            +G +F+IP  S+S F  +++L    + +KL +P ARK T + +G T LQRIGIGLV SI
Sbjct: 370 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 429

Query: 434 FGMVAAGVVEKERRTLALGQR-------TLSAFWLIPQFFLIGAGEAFTYVGQLEFFIRE 493
           F MV+AG++E  R                ++ FW +PQ+FL+G  E FT++GQLEFF  +
Sbjct: 430 FSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQ 489

Query: 494 APERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK----QWLRSNLNMGNLNYFYWLL 553
           AP+ M+S+ + L L+ ++ G ++S+ LVT+V KVT       W+  NLN G+L+YF+WLL
Sbjct: 490 APDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLL 549

Query: 554 AVLGLLNFFFFLVLARKHQYK 555
           A L  LNF  +L +A+ + YK
Sbjct: 550 AGLSFLNFLVYLWIAKWYTYK 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVE03.4e-23474.37Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q050856.1e-15150.81Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9SZY41.9e-14751.65Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q9M3902.9e-12443.85Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB81.7e-11641.53Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FWL40.0e+00100.00protein NRT1/ PTR FAMILY 6.4-like OS=Cucurbita moschata OX=3662 GN=LOC111448847 ... [more]
A0A6J1JG190.0e+0098.98protein NRT1/ PTR FAMILY 6.4-like OS=Cucurbita maxima OX=3661 GN=LOC111484070 PE... [more]
A0A5D3CCF73.3e-30991.71Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3C2633.3e-30991.71protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo OX=3656 GN=LOC103495995 PE=3 SV=1[more]
A0A5A7V3P81.7e-30891.54Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
Match NameE-valueIdentityDescription
XP_022944384.10.0e+00100.00protein NRT1/ PTR FAMILY 6.4-like [Cucurbita moschata][more]
KAG6570905.10.0e+0099.66Protein NRT1/ PTR FAMILY 6.4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023512951.10.0e+0099.15protein NRT1/ PTR FAMILY 6.4-like [Cucurbita pepo subsp. pepo][more]
XP_022986273.10.0e+0098.98protein NRT1/ PTR FAMILY 6.4-like [Cucurbita maxima][more]
XP_008455932.16.8e-30991.71PREDICTED: protein NRT1/ PTR FAMILY 6.4 [Cucumis melo] >TYK09533.1 protein NRT1/... [more]
Match NameE-valueIdentityDescription
AT3G21670.12.4e-23574.37Major facilitator superfamily protein [more]
AT1G12110.14.4e-15250.81nitrate transporter 1.1 [more]
AT2G26690.11.3e-14851.65Major facilitator superfamily protein [more]
AT3G54140.12.0e-12543.85peptide transporter 1 [more]
AT5G01180.11.2e-11741.53peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 9..560
e-value: 6.0E-182
score: 607.5
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 28..555
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 101..514
e-value: 5.4E-109
score: 364.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 13..565
NoneNo IPR availablePANTHERPTHR11654:SF373PROTEIN NRT1/ PTR FAMILY 6.4-LIKEcoord: 13..565

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G006470.1CmoCh20G006470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity