CmoCh20G006290 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh20G006290
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Membrane metalloprotease ARASP, chloroplastic
Location	Cmo_Chr20: 3091878 .. 3093227 (+)
RNA-Seq Expression	CmoCh20G006290
Synteny	CmoCh20G006290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTATTAAATCTCTCTTCCTCTTCTTATTCTCTCAGTAAATTTGCGAATTCGAGGTCACCCTTCTCAGATTTTTCAGTTAAGTACAGAACCCACTTCTCTAAATCCCAATCTTCTTCTTCATTATGTAATTCTTTAACGTCATCGGAGCTCAGTTTCAAGAACAGACACCATATCTTCTGGGAAAAGAGCAAAATCCCATGTCGGAGGAGCGGCGATTTCAGGCTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTCGAGGCGGCGGCTGTGTTGACGGCCATCATTGTCGTTCACGAGAGTGGCCACTTCCTCGCCGCTTGTCTACAGGGTATCCATGTAAGTAAATTTGCAATTGGTTTTGGTCCAGTTCTTGCAAAATTCAACAAAAACAATGTAGAATACTCTATTAGGGCTTTTCCTCTTGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGATAGCGATATTCCAATGGATGACGAAAATTTGCTTAAAAATAGACCAATATTGGATAGAGTATTAGTGATTTCTGCTGGTGTTATTGCTAATATAATATTCGCATACATAATCATCTTAATCCAAGTCTCGTCGGTTGGTTTGCCGGTTCAAGAGCCCTTTCCCGGCGTGCTTGTGCCTGAGGTTCGAACCCTTTCGGCTGCTTCTCGAGATGGGCTGCTTCCAGGGGATGTAATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCCACAGCCGTTTCTGAGCTTGTTGAGGCAATTAAAAGAAGCCCCAATAGAACTGTTCTTCTCAAAGTTGAAAGAGGGCAACAGGATCTTGAAATTGGGGTCACTCCAGATGAGAGTTTTGATGGAACAGGGCGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAGCTTGTAGCGAAGAATTTCTTGGAGGCTTTTAGCTATTCTAGGAAGGAATTTTTGGGTCTGTCATATAATGTTCTTGATAGCTTGAAACAAACTGTTCTGAATATCTCTCAGTCAGCCAGTAAGGTTTCTGGTCCTGTGGCGATTATTGCTGTTGGTGCTGAAGTTGCAAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAATATTAACCTTGCTGTTATAAACCTTCTGCCCTTGCCTGCTTTGGATGGCGGCTCGTTGGCTTTGATCCTCATTGAGGCTGCAAGGGGAGGGAGGAAGTTGCCTCTAGAACTCGAGCAGCGAATCATGTCGTCTGGGATCATGGTTGTTGTACTTCTTGGGATGTTCCTCATTGTCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

mRNA sequence

ATGCTATTAAATCTCTCTTCCTCTTCTTATTCTCTCAGTAAATTTGCGAATTCGAGGTCACCCTTCTCAGATTTTTCAGTTAAGTACAGAACCCACTTCTCTAAATCCCAATCTTCTTCTTCATTATGTAATTCTTTAACGTCATCGGAGCTCAGTTTCAAGAACAGACACCATATCTTCTGGGAAAAGAGCAAAATCCCATGTCGGAGGAGCGGCGATTTCAGGCTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTCGAGGCGGCGGCTGTGTTGACGGCCATCATTGTCGTTCACGAGAGTGGCCACTTCCTCGCCGCTTGTCTACAGGGTATCCATGTAAGTAAATTTGCAATTGGTTTTGGTCCAGTTCTTGCAAAATTCAACAAAAACAATGTAGAATACTCTATTAGGGCTTTTCCTCTTGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGATAGCGATATTCCAATGGATGACGAAAATTTGCTTAAAAATAGACCAATATTGGATAGAGTATTAGTGATTTCTGCTGGTGTTATTGCTAATATAATATTCGCATACATAATCATCTTAATCCAAGTCTCGTCGGTTGGTTTGCCGGTTCAAGAGCCCTTTCCCGGCGTGCTTGTGCCTGAGGTTCGAACCCTTTCGGCTGCTTCTCGAGATGGGCTGCTTCCAGGGGATGTAATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCCACAGCCGTTTCTGAGCTTGTTGAGGCAATTAAAAGAAGCCCCAATAGAACTGTTCTTCTCAAAGTTGAAAGAGGGCAACAGGATCTTGAAATTGGGGTCACTCCAGATGAGAGTTTTGATGGAACAGGGCGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAGCTTGTAGCGAAGAATTTCTTGGAGGCTTTTAGCTATTCTAGGAAGGAATTTTTGGGTCTGTCATATAATGTTCTTGATAGCTTGAAACAAACTGTTCTGAATATCTCTCAGTCAGCCAGTAAGGTTTCTGGTCCTGTGGCGATTATTGCTGTTGGTGCTGAAGTTGCAAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAATATTAACCTTGCTGTTATAAACCTTCTGCCCTTGCCTGCTTTGGATGGCGGCTCGTTGGCTTTGATCCTCATTGAGGCTGCAAGGGGAGGGAGGAAGTTGCCTCTAGAACTCGAGCAGCGAATCATGTCGTCTGGGATCATGGTTGTTGTACTTCTTGGGATGTTCCTCATTGTCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

Coding sequence (CDS)

ATGCTATTAAATCTCTCTTCCTCTTCTTATTCTCTCAGTAAATTTGCGAATTCGAGGTCACCCTTCTCAGATTTTTCAGTTAAGTACAGAACCCACTTCTCTAAATCCCAATCTTCTTCTTCATTATGTAATTCTTTAACGTCATCGGAGCTCAGTTTCAAGAACAGACACCATATCTTCTGGGAAAAGAGCAAAATCCCATGTCGGAGGAGCGGCGATTTCAGGCTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTCGAGGCGGCGGCTGTGTTGACGGCCATCATTGTCGTTCACGAGAGTGGCCACTTCCTCGCCGCTTGTCTACAGGGTATCCATGTAAGTAAATTTGCAATTGGTTTTGGTCCAGTTCTTGCAAAATTCAACAAAAACAATGTAGAATACTCTATTAGGGCTTTTCCTCTTGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGATAGCGATATTCCAATGGATGACGAAAATTTGCTTAAAAATAGACCAATATTGGATAGAGTATTAGTGATTTCTGCTGGTGTTATTGCTAATATAATATTCGCATACATAATCATCTTAATCCAAGTCTCGTCGGTTGGTTTGCCGGTTCAAGAGCCCTTTCCCGGCGTGCTTGTGCCTGAGGTTCGAACCCTTTCGGCTGCTTCTCGAGATGGGCTGCTTCCAGGGGATGTAATTCTTGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCCACAGCCGTTTCTGAGCTTGTTGAGGCAATTAAAAGAAGCCCCAATAGAACTGTTCTTCTCAAAGTTGAAAGAGGGCAACAGGATCTTGAAATTGGGGTCACTCCAGATGAGAGTTTTGATGGAACAGGGCGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAGCTTGTAGCGAAGAATTTCTTGGAGGCTTTTAGCTATTCTAGGAAGGAATTTTTGGGTCTGTCATATAATGTTCTTGATAGCTTGAAACAAACTGTTCTGAATATCTCTCAGTCAGCCAGTAAGGTTTCTGGTCCTGTGGCGATTATTGCTGTTGGTGCTGAAGTTGCAAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAATATTAACCTTGCTGTTATAAACCTTCTGCCCTTGCCTGCTTTGGATGGCGGCTCGTTGGCTTTGATCCTCATTGAGGCTGCAAGGGGAGGGAGGAAGTTGCCTCTAGAACTCGAGCAGCGAATCATGTCGTCTGGGATCATGGTTGTTGTACTTCTTGGGATGTTCCTCATTGTCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTGTTATGA

Protein sequence

MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL

Homology

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BLAST of CmoCh20G006290 vs. ExPASy Swiss-Prot
Match: O23053 (Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP2 PE=2 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 3.6e-159
Identity = 308/455 (67.69%), Postives = 367/455 (80.66%), Query Frame = 0


Query: 1   MLLNLSSSSYS------LSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFK 60
           MLLN+SSS  S      LS F N   P S+F  K +TH  KS  S+   +SL      + 
Sbjct: 1   MLLNISSSPISHRIPHFLSDFNN---PTSNFPPKSKTHLPKSNLSTLSNHSL------YG 60

Query: 61  NRHHIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACL 120
            ++  F++  + P  R+      A    D GS +SVLEA+AVLTAIIVVHE+GHFLAA L
Sbjct: 61  TKNRAFYKNKRNPYNRT-----QALGRFDFGSLESVLEASAVLTAIIVVHETGHFLAASL 120

Query: 121 QGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRP 180
           QGI VSKFAIGFGP+LAKFN NNVEYS+RAFPLGGFVGFPDNDPDSDIP+DD NLLKNRP
Sbjct: 121 QGIRVSKFAIGFGPILAKFNSNNVEYSLRAFPLGGFVGFPDNDPDSDIPVDDRNLLKNRP 180

Query: 181 ILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPG 240
           ILDRV+V+SAG++AN+IFAY II  QV SVGLPVQE FPGVLVP+V++ SAASRDGLLPG
Sbjct: 181 ILDRVIVVSAGIVANVIFAYAIIFTQVVSVGLPVQESFPGVLVPDVKSFSAASRDGLLPG 240

Query: 241 DVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGR 300
           DVILAV+G EL   GS +VS++V+ +KR+P   VLL++ERG++  EI +TPD+SFDGTG+
Sbjct: 241 DVILAVDGTELSNSGSDSVSKVVDVVKRNPEHNVLLRIERGKESFEIRITPDKSFDGTGK 300

Query: 301 IGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVA 360
           IGVQLSPNV+  K+  KN  E FS++ +EF GLSYNVLDSLKQT LN SQ+ASKV+GPVA
Sbjct: 301 IGVQLSPNVRFGKVRPKNIPETFSFAGREFFGLSYNVLDSLKQTFLNFSQTASKVAGPVA 360

Query: 361 IIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLE 420
           IIAVGAEVARSN DGLYQFAA+LN+NLAVINLLPLPALDGG+LALIL+EA RGGRKLPLE
Sbjct: 361 IIAVGAEVARSNADGLYQFAALLNLNLAVINLLPLPALDGGTLALILLEAVRGGRKLPLE 420

Query: 421 LEQRIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           +EQ IMSSGIM+V+ LG+FLIV+DTLNL+FIK++L
Sbjct: 421 VEQGIMSSGIMLVLFLGLFLIVKDTLNLDFIKEML 441

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BLAST of CmoCh20G006290 vs. ExPASy Swiss-Prot
Match: O80885 (Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP PE=2 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 3.3e-157
Identity = 304/452 (67.26%), Postives = 363/452 (80.31%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSK---FANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRH 60
           MLLN+SSS  S       +N  +P S F  + +TH SKS            S  S KNR 
Sbjct: 1   MLLNISSSPISHRNPHFLSNFNNPISYFPRRSKTHLSKSHFFPKF---TPLSNQSLKNR- 60

Query: 61  HIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGI 120
            + +   + P     DFR  A +GID+GS +SVLEA AVLT IIVVHESGHFLAA LQGI
Sbjct: 61  -VLFGNKRYPDGERFDFRSRAISGIDLGSFESVLEAIAVLTTIIVVHESGHFLAASLQGI 120

Query: 121 HVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILD 180
           HVSKFAIGFGP+LAKF+ NNVEYS+RAFPLGGFVGFPDNDPDS+IP+DDENLLKNRP LD
Sbjct: 121 HVSKFAIGFGPILAKFDYNNVEYSLRAFPLGGFVGFPDNDPDSEIPIDDENLLKNRPTLD 180

Query: 181 RVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVI 240
           R +V+SAG+IAN+IFAY II +QV SVGLPVQE FPGVLVPEV+T SAASRDGLL GDVI
Sbjct: 181 RSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEVKTFSAASRDGLLSGDVI 240

Query: 241 LAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGV 300
           LAV+G EL K G  AVS++V+ +KR+P   V+ ++ERG +D +I VTPD++FDGTG+IGV
Sbjct: 241 LAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTPDKNFDGTGKIGV 300

Query: 301 QLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIA 360
           QLSPNV+I+K+  +N  E F +  +EF+GLS NVLD LKQT  N SQ+ASKV+GPVAIIA
Sbjct: 301 QLSPNVRITKVRPRNIPETFRFVGREFMGLSSNVLDGLKQTFFNFSQTASKVAGPVAIIA 360

Query: 361 VGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQ 420
           VGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALIL+EA RGG+KLP+E+EQ
Sbjct: 361 VGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALILLEAVRGGKKLPVEVEQ 420

Query: 421 RIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
            IMSSGIM+V+ LG+FLIV+DTL+L+FIK++L
Sbjct: 421 GIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 447

[Close]

BLAST of CmoCh20G006290 vs. ExPASy Swiss-Prot
Match: P73714 (Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1821 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.8e-89
Identity = 177/364 (48.63%), Postives = 254/364 (69.78%), Query Frame = 0


Query: 86  SAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPVLAKFNKNNVEYSIRAF 145
           S  + L A  VL  +I VHE GHF AA LQGIHV++FA+GFGP L K+     EYSIRA 
Sbjct: 2   SVLAALAAIGVLAVLIAVHELGHFAAARLQGIHVTRFALGFGPPLLKYQGAETEYSIRAI 61

Query: 146 PLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVLVISAGVIANIIFAYIIILIQVSSVG 205
           PLGG+V FPD+DPDS+IP DD NLLKNRPILDR +VISAGVIAN++FAY +++ QVS++G
Sbjct: 62  PLGGYVAFPDDDPDSEIPADDPNLLKNRPILDRAIVISAGVIANLVFAYFLLIGQVSTIG 121

Query: 206 LPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEAIKRSPN 265
              Q   PG+++P+V + SAA   G+ PGD++L++ GN LP     A ++ ++ ++RSP+
Sbjct: 122 F--QNIQPGLVIPQVDSASAAQVAGMEPGDIVLSLQGNTLPGF-PDATTQFIDIVRRSPS 181

Query: 266 RTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFL 325
             + ++V+RG++   + +TP    +G G+IGV L PNV+  +  A N LEA +YS + F 
Sbjct: 182 VPITVEVQRGEETKTLTITPTPDAEGKGKIGVALLPNVETKR--ASNPLEALTYSAEAFE 241

Query: 326 GLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVIN 385
            +         Q + N + +AS+V+GPV I+  GA +ARS+   L+QF A+++INLAVIN
Sbjct: 242 RIVKLTTQGFWQLISNFADNASQVAGPVKIVEYGANIARSDASNLFQFGALISINLAVIN 301

Query: 386 LLPLPALDGGSLALILIEAARGGRKLPLELEQRIMSSGIMVVVLLGMFLIVRDTLNLEFI 445
           +LPLPALDGG L  +LIE    G+ LP + +  +M +G+++++ LG+FLIVRDTLNL F+
Sbjct: 302 ILPLPALDGGQLVFLLIEGLL-GKPLPEKFQMGVMQTGLVLLLSLGVFLIVRDTLNLTFV 359

Query: 446 KDLL 450
           ++ L
Sbjct: 362 QEFL 359

[Close]

BLAST of CmoCh20G006290 vs. ExPASy Swiss-Prot
Match: Q8YQ64 (Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=all3971 PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 6.7e-81
Identity = 176/358 (49.16%), Postives = 243/358 (67.88%), Query Frame = 0


Query: 89  SVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLG 148
           SVL A AVL  +I+VHE GHF+AA  QGIHV++F++GFGPVL K+     EY+IRAFPLG
Sbjct: 2   SVLAAIAVLAVLILVHELGHFVAARSQGIHVNRFSLGFGPVLWKYQGAETEYAIRAFPLG 61

Query: 149 GFVGFPDNDPDSDIPMDDENLLKNRPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPV 208
           GFVGFPD+DPDSDIP +D NLL+NRPILDR +VISAGVIAN+IFAY+++L QV  VG+  
Sbjct: 62  GFVGFPDDDPDSDIPPNDPNLLRNRPILDRAIVISAGVIANLIFAYMLLLAQVGFVGIG- 121

Query: 209 QEPFPGV----LVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEAIKRSP 268
           Q   PGV    L PEV   + A+  GL PGDVIL+ N  E        +  L + IK SP
Sbjct: 122 QASQPGVSIQQLAPEVS--AVATNAGLKPGDVILSANQKEF-GTSLQEIEALRDIIKNSP 181

Query: 269 NRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLSPNVKISKLVAKNFLEAFSYSRKEF 328
            +++ L V RG + L + V P+    G G IG+ L+PN K+ +    +  +AFS    EF
Sbjct: 182 GKSIQLTVARGDERLSVNVIPEAKPAG-GSIGIGLAPNGKVERRPV-SLSKAFSVGASEF 241

Query: 329 LGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVI 388
             +         Q V N  ++AS+V+GP+ I+ +GA +A+++   L+ FAA+++INLAVI
Sbjct: 242 QRIVVMTFKGFGQLVTNFGETASQVAGPIKIVEIGANIAQNDTGSLFFFAALISINLAVI 301

Query: 389 NLLPLPALDGGSLALILIEAARGGRKLPLELEQRIMSSGIMVVVLLGMFLIVRDTLNL 443
           N+LPLPALDGG LA +LIE  R G+ LP  +++ +M +G+++++ LG+FLIV++T  L
Sbjct: 302 NILPLPALDGGQLAFLLIEGLR-GKPLPNRIQEGVMQTGLVLLLGLGIFLIVKETTQL 352

[Close]

BLAST of CmoCh20G006290 vs. ExPASy Swiss-Prot
Match: O31754 (Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 GN=rasP PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.1e-30
Identity = 123/435 (28.28%), Postives = 190/435 (43.68%), Query Frame = 0


Query: 89  SVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLG 148
           +V+    +   ++  HE GH L A   GI   +FAIGFGP +  F KN   Y+IR  P+G
Sbjct: 5   TVIAFIIIFGTLVFFHELGHLLLAQRAGILCREFAIGFGPKIFSFKKNETVYTIRLLPVG 64

Query: 149 GFVGFPDNDPD-----------------------------------------SDIPMD-- 208
           GFV     DP+                                         +D+  D  
Sbjct: 65  GFVRMAGEDPEMIEVKPGYTVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLEHDMK 124

Query: 209 ---------DE-------------------------NLLKNRPILDRVLVISAGVIANII 268
                    DE                             ++P+  R+  I+AG I N I
Sbjct: 125 ITGYEQGKEDELSSFTVSETSFFIVDGEEVQIAPYNRQFGSKPVWQRIKAIAAGPIMNFI 184

Query: 269 FAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAVNGNELPKLGST 328
            AY+I+++     G+P  EP  G L    R    A+  GL  GD I ++NG ++      
Sbjct: 185 LAYVILVMLGLIQGVPSNEPMLGQLTDNGR----AAEAGLKEGDYIQSINGEKM-----R 244

Query: 329 AVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTP----DESFDGTGRIGVQLSPNVKISK 388
           + +++V A+K +P + + + V+R  + L I VTP    DE+    GR G   +P      
Sbjct: 245 SWTDIVSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDENKKTIGRFG-SYAP------ 304

Query: 389 LVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGAEVARSNI 443
              K  L A +Y     + ++  +L +L + V         +SGPV I  +  +VA++ I
Sbjct: 305 -TEKGVLSAVAYGATSTVDVTKAILTNLSKLVTG-QFKLDMLSGPVGIYDMTDQVAKTGI 364

[Close]

BLAST of CmoCh20G006290 vs. ExPASy TrEMBL
Match: A0A6J1FWS5 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448141 PE=3 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 2.8e-239
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60
           MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF
Sbjct: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLGMFLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmoCh20G006290 vs. ExPASy TrEMBL
Match: A0A6J1JFQ4 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484092 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.0e-233
Identity = 438/449 (97.55%), Postives = 443/449 (98.66%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60
           MLLNLSSSSYS+ KFANS+SPFSDFSVKYRTHFSKSQ SSSLC+SLTSSELSFKNRH IF
Sbjct: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLG TAVSELVE IKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLN SQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLG+FLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmoCh20G006290 vs. ExPASy TrEMBL
Match: A0A0A0LT84 (PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.6e-218
Identity = 414/456 (90.79%), Postives = 432/456 (94.74%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSK-------SQSSSSLCNSLTSSELSF 60
           MLLNL SSS+S+ K AN +SPFSDFSVK RTHFSK       S SSSSL +S  SSELSF
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSLWHSPPSSELSF 60

Query: 61  KNRHHIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAAC 120
           K+RH IFW+K+KI CRR+GDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAAC
Sbjct: 61  KDRHQIFWDKTKIRCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAAC 120

Query: 121 LQGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNR 180
           LQGIHVSKFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIP+DDENLLKNR
Sbjct: 121 LQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPVDDENLLKNR 180

Query: 181 PILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLP 240
           PILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLLP
Sbjct: 181 PILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLP 240

Query: 241 GDVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTG 300
           GDVILAVNGNELPKLGSTAVS+LVEAIKRSPNRTVLLKVERG QDLEIGVTPD+SFDGTG
Sbjct: 241 GDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTG 300

Query: 301 RIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPV 360
           RIGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSYNVLDSLKQT LN SQSASKVSGPV
Sbjct: 301 RIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPV 360

Query: 361 AIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPL 420
           AIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLPL
Sbjct: 361 AIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPL 420

Query: 421 ELEQRIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           ELEQRIMSSG+M VVLLG+FLI+RDTLNLEFIKDLL
Sbjct: 421 ELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 456

[Close]

BLAST of CmoCh20G006290 vs. ExPASy TrEMBL
Match: A0A5D3E082 (Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3695G00250 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.3e-217
Identity = 412/457 (90.15%), Postives = 431/457 (94.31%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSK--------SQSSSSLCNSLTSSELS 60
           MLLNL SSS+S+ K AN +SPFSDFSVK RTHFSK        S SSSSL +S  SSELS
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSSLWHSPPSSELS 60

Query: 61  FKNRHHIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRH IFW+K+KI CRR+GDFRLWAFAG+DIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWDKTKIRCRRNGDFRLWAFAGMDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKN 180
           CLQGIHVSKFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIP+DDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELP LGSTAVS+LVEAIKRSPNRTVLLKVERG QDLEIGVTPD+SFDGT
Sbjct: 241 PGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGT 300

Query: 301 GRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGP 360
           GRIGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSYNVLDSLKQT LN SQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           LELEQRIMSSG+M VVLLG+FLI+RDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

[Close]

BLAST of CmoCh20G006290 vs. ExPASy TrEMBL
Match: A0A1S3C376 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495966 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.3e-217
Identity = 412/457 (90.15%), Postives = 431/457 (94.31%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSK--------SQSSSSLCNSLTSSELS 60
           MLLNL SSS+S+ K AN +SPFSDFSVK RTHFSK        S SSSSL +S  SSELS
Sbjct: 1   MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSSLWHSPPSSELS 60

Query: 61  FKNRHHIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120
           FKNRH IFW+K+KI CRR+GDFRLWAFAG+DIGSAQSVLEAAAVLTAIIVVHESGHFLAA
Sbjct: 61  FKNRHQIFWDKTKIRCRRNGDFRLWAFAGMDIGSAQSVLEAAAVLTAIIVVHESGHFLAA 120

Query: 121 CLQGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKN 180
           CLQGIHVSKFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIP+DDENLLKN
Sbjct: 121 CLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKN 180

Query: 181 RPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLL 240
           RPILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEV TLSAASRDGLL
Sbjct: 181 RPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLL 240

Query: 241 PGDVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGT 300
           PGDVILAVNGNELP LGSTAVS+LVEAIKRSPNRTVLLKVERG QDLEIGVTPD+SFDGT
Sbjct: 241 PGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGT 300

Query: 301 GRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGP 360
           GRIGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSYNVLDSLKQT LN SQSASKVSGP
Sbjct: 301 GRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGP 360

Query: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLP 420
           VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLP
Sbjct: 361 VAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLP 420

Query: 421 LELEQRIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           LELEQRIMSSG+M VVLLG+FLI+RDTLNLEFIKDLL
Sbjct: 421 LELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

[Close]

BLAST of CmoCh20G006290 vs. NCBI nr
Match: XP_022943353.1 (probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 837.4 bits (2162), Expect = 5.7e-239
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60
           MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF
Sbjct: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLGMFLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmoCh20G006290 vs. NCBI nr
Match: KAG6570888.1 (Membrane metalloprotease ARASP, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 823.2 bits (2125), Expect = 1.1e-234
Identity = 443/449 (98.66%), Postives = 445/449 (99.11%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60
           MLLNLSSSSYSL KFANSRSPF+DFSVKYRTHFSKSQSSSSLC+SLTSSELSFKNRH IF
Sbjct: 1   MLLNLSSSSYSLIKFANSRSPFTDFSVKYRTHFSKSQSSSSLCHSLTSSELSFKNRHQIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIP RRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPFRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLN SQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLGMFLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmoCh20G006290 vs. NCBI nr
Match: XP_023512495.1 (probable membrane metalloprotease ARASP2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512496.1 probable membrane metalloprotease ARASP2, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 820.1 bits (2117), Expect = 9.5e-234
Identity = 440/449 (98.00%), Postives = 444/449 (98.89%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60
           MLLNLSSSSYSL KFANS+SPFSD SVKYRTHFSKSQSSSSLC+SLTSSELSFKNRH IF
Sbjct: 1   MLLNLSSSSYSLIKFANSKSPFSDVSVKYRTHFSKSQSSSSLCHSLTSSELSFKNRHQIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKN LEAFSYSRKEFLGLSYNVLDSLKQTVLN SQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNSLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLG+FLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmoCh20G006290 vs. NCBI nr
Match: XP_022986309.1 (probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 818.9 bits (2114), Expect = 2.1e-233
Identity = 438/449 (97.55%), Postives = 443/449 (98.66%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRHHIF 60
           MLLNLSSSSYS+ KFANS+SPFSDFSVKYRTHFSKSQ SSSLC+SLTSSELSFKNRH IF
Sbjct: 1   MLLNLSSSSYSVIKFANSKSPFSDFSVKYRTHFSKSQFSSSLCHSLTSSELSFKNRHQIF 60

Query: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120
           WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS
Sbjct: 61  WEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVS 120

Query: 121 KFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180
           KFAIGFGP+LAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL
Sbjct: 121 KFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILDRVL 180

Query: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240
           VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV
Sbjct: 181 VISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVILAV 240

Query: 241 NGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300
           NGNELPKLG TAVSELVE IKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS
Sbjct: 241 NGNELPKLGPTAVSELVETIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGVQLS 300

Query: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIAVGA 360
           PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLN SQSASKVSGPVAIIAVGA
Sbjct: 301 PNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNFSQSASKVSGPVAIIAVGA 360

Query: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420
           EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM
Sbjct: 361 EVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQRIM 420

Query: 421 SSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           SSGIMVVVLLG+FLIVRDTLNLEFIKDLL
Sbjct: 421 SSGIMVVVLLGLFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of CmoCh20G006290 vs. NCBI nr
Match: XP_038902635.1 (probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida])

HSP 1 Score: 774.6 bits (1999), Expect = 4.6e-220
Identity = 414/455 (90.99%), Postives = 434/455 (95.38%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSKFANSRSPFSDFSVKYRTHFSK------SQSSSSLCNSLTSSELSFK 60
           ML+NLSSSS+S+ K A  +SPFSDFSVK RTHFSK      S SSSS C+S   S+LSFK
Sbjct: 1   MLVNLSSSSFSVIKLAKLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSFCHSFPLSDLSFK 60

Query: 61  NRHHIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACL 120
           NRH IFW+K+KIPCRR+GDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACL
Sbjct: 61  NRHQIFWDKTKIPCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACL 120

Query: 121 QGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRP 180
           QGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDP+SDIP+DDENLLKNRP
Sbjct: 121 QGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPESDIPVDDENLLKNRP 180

Query: 181 ILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPG 240
           ILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPG
Sbjct: 181 ILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPG 240

Query: 241 DVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGR 300
           DVILAVNGNELPKLGSTAVS+LVEAIKRSPNRTVLLKVERG+QDLEIGVTPDESFDGTGR
Sbjct: 241 DVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLEIGVTPDESFDGTGR 300

Query: 301 IGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVA 360
           IGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSY+VLDSLKQT LN SQSASKVSGPVA
Sbjct: 301 IGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYSVLDSLKQTFLNFSQSASKVSGPVA 360

Query: 361 IIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLE 420
           IIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEA RGGRKLPLE
Sbjct: 361 IIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPLE 420

Query: 421 LEQRIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           LEQRIMSSG+M VVLLG+FLI+RDTLNLEFIKDLL
Sbjct: 421 LEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 455

[Close]

BLAST of CmoCh20G006290 vs. TAIR 10
Match: AT1G05140.1 (Peptidase M50 family protein )

HSP 1 Score: 562.8 bits (1449), Expect = 2.5e-160
Identity = 308/455 (67.69%), Postives = 367/455 (80.66%), Query Frame = 0


Query: 1   MLLNLSSSSYS------LSKFANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFK 60
           MLLN+SSS  S      LS F N   P S+F  K +TH  KS  S+   +SL      + 
Sbjct: 1   MLLNISSSPISHRIPHFLSDFNN---PTSNFPPKSKTHLPKSNLSTLSNHSL------YG 60

Query: 61  NRHHIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACL 120
            ++  F++  + P  R+      A    D GS +SVLEA+AVLTAIIVVHE+GHFLAA L
Sbjct: 61  TKNRAFYKNKRNPYNRT-----QALGRFDFGSLESVLEASAVLTAIIVVHETGHFLAASL 120

Query: 121 QGIHVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRP 180
           QGI VSKFAIGFGP+LAKFN NNVEYS+RAFPLGGFVGFPDNDPDSDIP+DD NLLKNRP
Sbjct: 121 QGIRVSKFAIGFGPILAKFNSNNVEYSLRAFPLGGFVGFPDNDPDSDIPVDDRNLLKNRP 180

Query: 181 ILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPG 240
           ILDRV+V+SAG++AN+IFAY II  QV SVGLPVQE FPGVLVP+V++ SAASRDGLLPG
Sbjct: 181 ILDRVIVVSAGIVANVIFAYAIIFTQVVSVGLPVQESFPGVLVPDVKSFSAASRDGLLPG 240

Query: 241 DVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGR 300
           DVILAV+G EL   GS +VS++V+ +KR+P   VLL++ERG++  EI +TPD+SFDGTG+
Sbjct: 241 DVILAVDGTELSNSGSDSVSKVVDVVKRNPEHNVLLRIERGKESFEIRITPDKSFDGTGK 300

Query: 301 IGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVA 360
           IGVQLSPNV+  K+  KN  E FS++ +EF GLSYNVLDSLKQT LN SQ+ASKV+GPVA
Sbjct: 301 IGVQLSPNVRFGKVRPKNIPETFSFAGREFFGLSYNVLDSLKQTFLNFSQTASKVAGPVA 360

Query: 361 IIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLE 420
           IIAVGAEVARSN DGLYQFAA+LN+NLAVINLLPLPALDGG+LALIL+EA RGGRKLPLE
Sbjct: 361 IIAVGAEVARSNADGLYQFAALLNLNLAVINLLPLPALDGGTLALILLEAVRGGRKLPLE 420

Query: 421 LEQRIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
           +EQ IMSSGIM+V+ LG+FLIV+DTLNL+FIK++L
Sbjct: 421 VEQGIMSSGIMLVLFLGLFLIVKDTLNLDFIKEML 441

[Close]

BLAST of CmoCh20G006290 vs. TAIR 10
Match: AT2G32480.1 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 556.2 bits (1432), Expect = 2.4e-158
Identity = 304/452 (67.26%), Postives = 363/452 (80.31%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSK---FANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRH 60
           MLLN+SSS  S       +N  +P S F  + +TH SKS            S  S KNR 
Sbjct: 1   MLLNISSSPISHRNPHFLSNFNNPISYFPRRSKTHLSKSHFFPKF---TPLSNQSLKNR- 60

Query: 61  HIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGI 120
            + +   + P     DFR  A +GID+GS +SVLEA AVLT IIVVHESGHFLAA LQGI
Sbjct: 61  -VLFGNKRYPDGERFDFRSRAISGIDLGSFESVLEAIAVLTTIIVVHESGHFLAASLQGI 120

Query: 121 HVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILD 180
           HVSKFAIGFGP+LAKF+ NNVEYS+RAFPLGGFVGFPDNDPDS+IP+DDENLLKNRP LD
Sbjct: 121 HVSKFAIGFGPILAKFDYNNVEYSLRAFPLGGFVGFPDNDPDSEIPIDDENLLKNRPTLD 180

Query: 181 RVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVI 240
           R +V+SAG+IAN+IFAY II +QV SVGLPVQE FPGVLVPEV+T SAASRDGLL GDVI
Sbjct: 181 RSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEVKTFSAASRDGLLSGDVI 240

Query: 241 LAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGV 300
           LAV+G EL K G  AVS++V+ +KR+P   V+ ++ERG +D +I VTPD++FDGTG+IGV
Sbjct: 241 LAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTPDKNFDGTGKIGV 300

Query: 301 QLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIA 360
           QLSPNV+I+K+  +N  E F +  +EF+GLS NVLD LKQT  N SQ+ASKV+GPVAIIA
Sbjct: 301 QLSPNVRITKVRPRNIPETFRFVGREFMGLSSNVLDGLKQTFFNFSQTASKVAGPVAIIA 360

Query: 361 VGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQ 420
           VGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALIL+EA RGG+KLP+E+EQ
Sbjct: 361 VGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALILLEAVRGGKKLPVEVEQ 420

Query: 421 RIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
            IMSSGIM+V+ LG+FLIV+DTL+L+FIK++L
Sbjct: 421 GIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 447

[Close]

BLAST of CmoCh20G006290 vs. TAIR 10
Match: AT2G32480.2 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 503.4 bits (1295), Expect = 1.8e-142
Identity = 285/452 (63.05%), Postives = 339/452 (75.00%), Query Frame = 0


Query: 1   MLLNLSSSSYSLSK---FANSRSPFSDFSVKYRTHFSKSQSSSSLCNSLTSSELSFKNRH 60
           MLLN+SSS  S       +N  +P S F  + +TH SKS            S  S KNR 
Sbjct: 1   MLLNISSSPISHRNPHFLSNFNNPISYFPRRSKTHLSKSHFFPKF---TPLSNQSLKNR- 60

Query: 61  HIFWEKSKIPCRRSGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGI 120
            + +   + P     DFR  A +GID+GS +SVLEA AVLT IIVVHESGHFLAA LQGI
Sbjct: 61  -VLFGNKRYPDGERFDFRSRAISGIDLGSFESVLEAIAVLTTIIVVHESGHFLAASLQGI 120

Query: 121 HVSKFAIGFGPVLAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPMDDENLLKNRPILD 180
           HVSKFAIGFGP+LAKF+ NNVEYS+RAFPLGGFVGFPDNDPDS+IP+DDENLLKNRP LD
Sbjct: 121 HVSKFAIGFGPILAKFDYNNVEYSLRAFPLGGFVGFPDNDPDSEIPIDDENLLKNRPTLD 180

Query: 181 RVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEVRTLSAASRDGLLPGDVI 240
           R +V+SAG+IAN+IFAY II +QV SVGLPVQE FPGVLVPEV+T SAASRDGLL GDVI
Sbjct: 181 RSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEVKTFSAASRDGLLSGDVI 240

Query: 241 LAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLEIGVTPDESFDGTGRIGV 300
           LAV+G EL K G  AVS++V+ +KR+P   V+ ++ERG +D +I VTPD++FDGTG+IGV
Sbjct: 241 LAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTPDKNFDGTGKIGV 300

Query: 301 QLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVLNISQSASKVSGPVAIIA 360
           QLSPNV+I+K                                     +ASKV+GPVAIIA
Sbjct: 301 QLSPNVRITK-------------------------------------TASKVAGPVAIIA 360

Query: 361 VGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAARGGRKLPLELEQ 420
           VGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALIL+EA RGG+KLP+E+EQ
Sbjct: 361 VGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALILLEAVRGGKKLPVEVEQ 410

Query: 421 RIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 450
            IMSSGIM+V+ LG+FLIV+DTL+L+FIK++L
Sbjct: 421 GIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 410

The following BLAST results are available for this feature:

BLAST of CmoCh20G006290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23053	3.6e-159	67.69	Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana ...	[more]
O80885	3.3e-157	67.26	Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
P73714	1.8e-89	48.63	Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Ka...	[more]
Q8YQ64	6.7e-81	49.16	Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82...	[more]
O31754	1.1e-30	28.28	Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 G...	[more]

BLAST of CmoCh20G006290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FWS5	2.8e-239	100.00	probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita moschata OX...	[more]
A0A6J1JFQ4	1.0e-233	97.55	probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita maxima OX=3...	[more]
A0A0A0LT84	1.6e-218	90.79	PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV...	[more]
A0A5D3E082	1.3e-217	90.15	Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3C376	1.3e-217	90.15	probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 ...	[more]

BLAST of CmoCh20G006290 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943353.1	5.7e-239	100.00	probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita moschata]	[more]
KAG6570888.1	1.1e-234	98.66	Membrane metalloprotease ARASP, chloroplastic, partial [Cucurbita argyrosperma s...	[more]
XP_023512495.1	9.5e-234	98.00	probable membrane metalloprotease ARASP2, chloroplastic isoform X1 [Cucurbita pe...	[more]
XP_022986309.1	2.1e-233	97.55	probable membrane metalloprotease ARASP2, chloroplastic [Cucurbita maxima]	[more]
XP_038902635.1	4.6e-220	90.99	probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida]	[more]

BLAST of CmoCh20G006290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05140.1	2.5e-160	67.69	Peptidase M50 family protein	[more]
AT2G32480.1	2.4e-158	67.26	ARABIDOPSIS SERIN PROTEASE	[more]
AT2G32480.2	1.8e-142	63.05	ARABIDOPSIS SERIN PROTEASE	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001478	PDZ domain	SMART	SM00228	pdz_new	coord: 202..276 e-value: 1.3E-10 score: 51.3
IPR001478	PDZ domain	PROSITE	PS50106	PDZ	coord: 204..275 score: 11.4207
IPR008915	Peptidase M50	PFAM	PF02163	Peptidase_M50	coord: 94..430 e-value: 1.2E-59 score: 201.6
IPR004387	Peptidase M50, putative membrane-associated zinc metallopeptidase	TIGRFAM	TIGR00054	TIGR00054	coord: 93..247 e-value: 1.1E-43 score: 147.7
IPR004387	Peptidase M50, putative membrane-associated zinc metallopeptidase	PANTHER	PTHR42837	REGULATOR OF SIGMA-E PROTEASE RSEP	coord: 22..449
IPR041489	PDZ domain 6	PFAM	PF17820	PDZ_6	coord: 217..274 e-value: 9.9E-9 score: 35.0
IPR036034	PDZ superfamily	GENE3D	2.30.42.10		coord: 204..300 e-value: 5.1E-16 score: 60.8
IPR036034	PDZ superfamily	SUPERFAMILY	50156	PDZ domain-like	coord: 202..287
None	No IPR available	PANTHER	PTHR42837:SF4	MEMBRANE METALLOPROTEASE ARASP2, CHLOROPLASTIC-RELATED	coord: 22..449
None	No IPR available	CDD	cd06163	S2P-M50_PDZ_RseP-like	coord: 90..441 e-value: 8.52699E-39 score: 136.775
None	No IPR available	CDD	cd00989	PDZ_metalloprotease	coord: 202..285 e-value: 2.12671E-14 score: 66.1031

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh20G006290.1	CmoCh20G006290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0005515	protein binding