CmoCh20G006280 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G006280
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionO-methyltransferase family protein
LocationCmo_Chr20: 3090098 .. 3091251 (+)
RNA-Seq ExpressionCmoCh20G006280
SyntenyCmoCh20G006280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGGAAGCTGGATGAGTTGCTTCAATCTCAAGCACATGTTTGGAACCATGTTTTGAAAATTTTCAACTCCATGTCTCTTAAATGTGCTGTTGAATTGGGCATACCGGATGCTATCCATAGCCACGCCCAACCCATGAGCCTCTCTGATTTAGTGGCGGCGCTTCATATTCAACCTTCAAAGGCTCAACACCTTGGCCGCCTCATGCGCCTCCTCGTCCATTCTGGCTTCTTTGATGCGGACGAGCAAGAAGACGTGAGATACAGCCTCACGCCATCCTCTCGCCTTCTCCTCCGCCACACCGACACACACACTCACACCACTTTCCAAATCACACCCTTCTTGTTTCTGAGTTTGGATAAAACTACAATAGCTTCTTTGGGAGCTTTAAGCGGTTGGCTTTGTAGGTCAGAGGATGACCACAACCCCAGTGTCTTTGAAATGGCAAACGGGAAGCCCGTATGGGAGTATGCTGCTCAGGAGCCAAGTCTTGCCAATTTGTTTCAACAGACAATGGCTTGCGATTCTGAAATGATTGGTAAAATAGTGATTGAGAAGTGTAGCGGTGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCTACAACCAAATGTAAGAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGGTAATGTTCTTATATTAACAAATTAGATTCGTTTTAACACTAAGTTATTAATGGATTTTGATAATCAGTGGGTGTTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCTGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGTAAACGAAGTAGAAGACAGGGATTCCACTGAAACTCAACTGTTCTTTGATGTATTGATGATGGTCAATCTAAACGGCAAGGAGAGAAATGAGAAAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGGTCACTTATTGAGGTGTACCCTTAA

mRNA sequence

ATGGAAGGGAAGCTGGATGAGTTGCTTCAATCTCAAGCACATGTTTGGAACCATGTTTTGAAAATTTTCAACTCCATGTCTCTTAAATGTGCTGTTGAATTGGGCATACCGGATGCTATCCATAGCCACGCCCAACCCATGAGCCTCTCTGATTTAGTGGCGGCGCTTCATATTCAACCTTCAAAGGCTCAACACCTTGGCCGCCTCATGCGCCTCCTCGTCCATTCTGGCTTCTTTGATGCGGACGAGCAAGAAGACGTGAGATACAGCCTCACGCCATCCTCTCGCCTTCTCCTCCGCCACACCGACACACACACTCACACCACTTTCCAAATCACACCCTTCTTGTTTCTGAGTTTGGATAAAACTACAATAGCTTCTTTGGGAGCTTTAAGCGGTTGGCTTTGTAGGTCAGAGGATGACCACAACCCCAGTGTCTTTGAAATGGCAAACGGGAAGCCCGTATGGGAGTATGCTGCTCAGGAGCCAAGTCTTGCCAATTTGTTTCAACAGACAATGGCTTGCGATTCTGAAATGATTGGTAAAATAGTGATTGAGAAGTGTAGCGGTGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCTACAACCAAATGTAAGAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGGTGTTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCTGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGTAAACGAAGTAGAAGACAGGGATTCCACTGAAACTCAACTGTTCTTTGATGTATTGATGATGGTCAATCTAAACGGCAAGGAGAGAAATGAGAAAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGGTCACTTATTGAGGTGTACCCTTAA

Coding sequence (CDS)

ATGGAAGGGAAGCTGGATGAGTTGCTTCAATCTCAAGCACATGTTTGGAACCATGTTTTGAAAATTTTCAACTCCATGTCTCTTAAATGTGCTGTTGAATTGGGCATACCGGATGCTATCCATAGCCACGCCCAACCCATGAGCCTCTCTGATTTAGTGGCGGCGCTTCATATTCAACCTTCAAAGGCTCAACACCTTGGCCGCCTCATGCGCCTCCTCGTCCATTCTGGCTTCTTTGATGCGGACGAGCAAGAAGACGTGAGATACAGCCTCACGCCATCCTCTCGCCTTCTCCTCCGCCACACCGACACACACACTCACACCACTTTCCAAATCACACCCTTCTTGTTTCTGAGTTTGGATAAAACTACAATAGCTTCTTTGGGAGCTTTAAGCGGTTGGCTTTGTAGGTCAGAGGATGACCACAACCCCAGTGTCTTTGAAATGGCAAACGGGAAGCCCGTATGGGAGTATGCTGCTCAGGAGCCAAGTCTTGCCAATTTGTTTCAACAGACAATGGCTTGCGATTCTGAAATGATTGGTAAAATAGTGATTGAGAAGTGTAGCGGTGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCTACAACCAAATGTAAGAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGGTGTTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCTGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGTAAACGAAGTAGAAGACAGGGATTCCACTGAAACTCAACTGTTCTTTGATGTATTGATGATGGTCAATCTAAACGGCAAGGAGAGAAATGAGAAAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGGTCACTTATTGAGGTGTACCCTTAA

Protein sequence

MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVYP
Homology
BLAST of CmoCh20G006280 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 3.3e-99
Identity = 188/365 (51.51%), Postives = 248/365 (67.95%), Query Frame = 0

Query: 7   ELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHL 66
           E+L +QAH+WN +    NSMSLKCA++LGIPD I++H +PM++S L  AL I   K+  +
Sbjct: 25  EILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHGKPMTISQLTLALPINRKKSPCV 84

Query: 67  GRLMRLLVHSGFF-----DADEQ---EDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFL 126
            RLMR+L+HSGFF     +  E+   E+  Y +T +S+LLL+           +TPFL  
Sbjct: 85  YRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLK------DNPMSVTPFLLA 144

Query: 127 SLDKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSE 186
            LD         LS W      + +P+ F+ ANG   W+Y + EP LA  F   MA D+ 
Sbjct: 145 MLDPVMTKPWDFLSNWF----QNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMASDAR 204

Query: 187 MIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVR 246
           ++  +VIEKC GVFEG++SLVDVGGGTG +A++IA AFPHI C+VFDLP VV++LQ    
Sbjct: 205 LVTSVVIEKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGG-N 264

Query: 247 NLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAI-PSRAEGGKLIIIDM 306
           NL+F+GGDM    +P    V+LKW+LH+W+DE+S+KILKKCK+AI  S  +GGK+IIIDM
Sbjct: 265 NLNFVGGDMFVD-VPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDM 324

Query: 307 VLVNEV-EDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSL 362
            + NE  ED +S ETQLFFD+LMM  + G+ERNEKEW  LF +AGFS+Y I   LGLRSL
Sbjct: 325 KVENEKDEDDESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGLRSL 377

BLAST of CmoCh20G006280 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 354.4 bits (908), Expect = 1.6e-96
Identity = 178/359 (49.58%), Postives = 242/359 (67.41%), Query Frame = 0

Query: 7   ELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHL 66
           ELL +QAHVWNH+     SMSLKCA++LGIPD IH+H +PM+L +LVA L + P ++Q +
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHGKPMTLPELVAKLPVHPKRSQCV 70

Query: 67  GRLMRLLVHSGFFDADE----QEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSLDK 126
            RLMR+LVHSGF  A      +E+  Y LT +SRLLL         +  I P +   LD 
Sbjct: 71  YRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLL------MDDSLSIRPLVLAMLDP 130

Query: 127 TTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMIGK 186
                   LS W      + +P+ F  A  +  W+YA  EP L N F + MA D+ ++  
Sbjct: 131 ILTKPWHYLSAWF----QNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTS 190

Query: 187 IVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSF 246
           +++++  GVF GL SLVDVGGGTG +A AIA AFPH+ C+V DL  VV+ LQ + +NL++
Sbjct: 191 VLLKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGS-KNLNY 250

Query: 247 IGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLVNE 306
             GDM E+ IPPA+A++LKW+LH+W +E+ +KILK+C++AIPS+  GGK+IIIDM+++  
Sbjct: 251 FAGDMFEA-IPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKN 310

Query: 307 VEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVYP 362
             D  STETQLFFD+ MM+   G+ER+E EW+ LF++AGFS Y I   LGLRSLIEVYP
Sbjct: 311 QGDYKSTETQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

BLAST of CmoCh20G006280 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 6.3e-90
Identity = 174/362 (48.07%), Postives = 246/362 (67.96%), Query Frame = 0

Query: 6   DELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSH-AQPMSLSDLVAALHIQPSKAQ 65
           +ELL +QAH++NH+    +SM+LKCAVEL IP+ +H+H  + ++LS+LV AL I  +K+ 
Sbjct: 22  EELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTLSELVQALQIPQAKSA 81

Query: 66  HLGRLMRLLVHSGFFD----ADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 125
            L RL+R+LVHSGFF       E ++  Y  T SS+LLL+      +    ++P L   +
Sbjct: 82  CLYRLLRILVHSGFFAITKIQSEGDEEGYLPTLSSKLLLK------NHPMSMSPCLLGLV 141

Query: 126 DKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI 185
           + T +A +   S W  RS DD  P  FE  +G  +W+Y  + P +A +F + M C++ + 
Sbjct: 142 NPTMVAPMHFFSDWFKRS-DDMTP--FEATHGASLWKYFGETPHMAEIFNEAMGCETRLA 201

Query: 186 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 245
             +V+++C G  EG+ SLVDVGGGTG +  AIAEAFP++ C+V DLPQVV NL+ +  NL
Sbjct: 202 MSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGS-NNL 261

Query: 246 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV 305
            F+ GDM +  IPPA+ V LKW+LH+W+DE+ IKILK+CK+AIPS+ EGGKLIIIDMV+ 
Sbjct: 262 EFVSGDMFQF-IPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDMVVN 321

Query: 306 NEVE-DRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEV 362
           +  +   +STETQLF+D+ +M  L G ER E EWK LF+ AGF+ Y I   LGL+S+IEV
Sbjct: 322 DHNKGSYESTETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLGLKSIIEV 372

BLAST of CmoCh20G006280 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.7e-87
Identity = 172/368 (46.74%), Postives = 238/368 (64.67%), Query Frame = 0

Query: 7   ELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHL 66
           ELL++QAHVWNH+    NSMSLKCA++LGIPDAIH H  P++LS L  AL+I  +K+  L
Sbjct: 12  ELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHGNPITLSQLADALNINKAKSHGL 71

Query: 67  GRLMRLLVHSGFFDA-----------DEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPF 126
            RLMR+LVHSGFFD            +E+E+  YSLTP+SRLLLR           + PF
Sbjct: 72  FRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLR------SEPLSVAPF 131

Query: 127 LFLSLDKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMAC 186
                D     +   LS W      +   + F+   G    EYA  +  L  LF + MAC
Sbjct: 132 ALAMSDPVYTETWHHLSEWF----RNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMAC 191

Query: 187 DSEMIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQP 246
           D+  +  I+  +C  +F+GL+S+VDVGGGTG  A  IA AFP + C+V DLP VV  L+ 
Sbjct: 192 DAGFVNSILTTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKG 251

Query: 247 NVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGG--KLI 306
           +  NLSF+ GDM +  IP A+A+ +K++LH+W+DE+ +KILKKCK+AI SR+     K+I
Sbjct: 252 S-ENLSFVSGDMFDF-IPHADAIFMKFILHDWNDEECVKILKKCKEAI-SRSNNSCRKII 311

Query: 307 IIDMVLVNEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGL 362
           ++++V+ +E E  ++TET+LFFD+ M+  + GKER+EKEW  LF +AGF++Y I   LGL
Sbjct: 312 LVEIVMEDEKETHEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITRVLGL 366

BLAST of CmoCh20G006280 vs. ExPASy Swiss-Prot
Match: Q8GSN1 (Myricetin O-methyltransferase OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 5.2e-84
Identity = 171/365 (46.85%), Postives = 241/365 (66.03%), Query Frame = 0

Query: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60
           ME +  E+  +QAH +  V    +  SLKCAV+LGIPDAIHSH +PM+LSDL  +L I P
Sbjct: 1   MELQSSEIRNAQAHFFTQVFSFTSMSSLKCAVQLGIPDAIHSHGKPMALSDLTNSLPINP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 120
           SKA ++ RLMR+LV +G+F   E+E   YSLTP +RLLL++   ++         + + L
Sbjct: 61  SKAPYIYRLMRILVAAGYF--SEEEKNVYSLTPFTRLLLKNDPLNS---------ISMVL 120

Query: 121 DKTTIASLGALSGWLCRSE--DDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSE 180
               IA L A   W   SE   + + + FE A+GK  W++ A++    N F   MA DS 
Sbjct: 121 GVNQIAELKA---WNAMSEWFQNEDLTAFETAHGKNFWDFGAEDKYGKN-FDGVMAADSI 180

Query: 181 MIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVR 240
           ++ K++I + + +FEGL SLVDVGGGTG +A AIA++FP + C+VFDLP VV+NL+ +  
Sbjct: 181 LVSKMLIPEFNYLFEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLE-STE 240

Query: 241 NLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMV 300
           NL F+GGDM E KIP ANA++LKW+LH+W DE+ +K+LK C+ AIP + +GGK+I+I+ V
Sbjct: 241 NLEFVGGDMFE-KIPSANAILLKWILHDWKDEECVKVLKMCRKAIPEKEKGGKVILIETV 300

Query: 301 LVN--EVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSL 360
           L++  + E+ ++ + Q+  D+ MMV    KER E+EW  LF EAGFS Y I   +  RS 
Sbjct: 301 LMDSKKHENEEAVKAQISSDIDMMVFFTAKERTEEEWATLFREAGFSGYKIFPMIDFRSP 348

Query: 361 IEVYP 362
           IEVYP
Sbjct: 361 IEVYP 348

BLAST of CmoCh20G006280 vs. ExPASy TrEMBL
Match: A0A6J1FTR0 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448368 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.3e-191
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 26  MSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHLGRLMRLLVHSGFFDADEQE 85
           MSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHLGRLMRLLVHSGFFDADEQE
Sbjct: 1   MSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHLGRLMRLLVHSGFFDADEQE 60

Query: 86  DVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRSEDDHNPS 145
           DVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRSEDDHNPS
Sbjct: 61  DVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRSEDDHNPS 120

Query: 146 VFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGT 205
           VFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGT
Sbjct: 121 VFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGT 180

Query: 206 GVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLH 265
           GVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLH
Sbjct: 181 GVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLH 240

Query: 266 NWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRDSTETQLFFDVLMMVNLNG 325
           NWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRDSTETQLFFDVLMMVNLNG
Sbjct: 241 NWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRDSTETQLFFDVLMMVNLNG 300

Query: 326 KERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVYP 362
           KERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVYP
Sbjct: 301 KERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVYP 336

BLAST of CmoCh20G006280 vs. ExPASy TrEMBL
Match: A0A6J1JFR9 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111484102 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 7.1e-185
Identity = 320/361 (88.64%), Postives = 336/361 (93.07%), Query Frame = 0

Query: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60
           ME KLDELLQSQAHVWNH LK  NSM+LKCAVELGIPD IHSHAQPM+LSDLVAAL IQP
Sbjct: 1   MEEKLDELLQSQAHVWNHALKFMNSMALKCAVELGIPDVIHSHAQPMTLSDLVAALRIQP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 120
           SKAQ+LGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTH+HTTFQI PFL+L L
Sbjct: 61  SKAQYLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHSHTTFQIIPFLYLGL 120

Query: 121 DKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI 180
           DKTTIASLGA SGWLC S+ DHN + FEMANGKP+WEYAAQEPS  NLFQQTM CDSEMI
Sbjct: 121 DKTTIASLGAFSGWLCSSDQDHNSNSFEMANGKPIWEYAAQEPSFGNLFQQTMVCDSEMI 180

Query: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 240
           GKIV EKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITC+VFDLPQVVSN Q   +NL
Sbjct: 181 GKIVTEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCTVFDLPQVVSNQQRTAKNL 240

Query: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV 300
            FIGGDMLE+KIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKL+IIDMVL 
Sbjct: 241 RFIGGDMLEAKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLVIIDMVLD 300

Query: 301 NEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY 360
           NE+E+R+STETQL FDVLMMVNL+GKERNEKEWKNLFMEAGFSDYTIV KLGLRSLIEVY
Sbjct: 301 NEMEERESTETQLLFDVLMMVNLHGKERNEKEWKNLFMEAGFSDYTIVCKLGLRSLIEVY 360

Query: 361 P 362
           P
Sbjct: 361 P 361

BLAST of CmoCh20G006280 vs. ExPASy TrEMBL
Match: A0A6J1FXQ6 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448676 PE=3 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.5e-182
Identity = 320/361 (88.64%), Postives = 336/361 (93.07%), Query Frame = 0

Query: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60
           ME K+DELLQSQAHVWNH LK+ NSM+LKCAVELGIPD IH+HAQPMSLSDLVAALHIQP
Sbjct: 1   MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 120
           SKAQ+LGRLMR LVHSGFFDADEQEDVRYSLTPSSRLLLRH  THT+TTFQ+ PFL ++L
Sbjct: 61  SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRH--THTYTTFQMIPFLCIAL 120

Query: 121 DKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI 180
           DK TIASLGALS WLC S+  HN + FEMANGK +WEYAAQEP+  NLFQQ M CDSE+I
Sbjct: 121 DKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELI 180

Query: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 240
           GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL
Sbjct: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 240

Query: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV 300
           SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSR EGGKLIIIDMVL 
Sbjct: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLD 300

Query: 301 NEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY 360
           NEVEDR+STETQLFFDVLMMVNLNGKERNEKEWK LFMEAGFSDYTI+ KLGLRSLIEVY
Sbjct: 301 NEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVY 359

Query: 361 P 362
           P
Sbjct: 361 P 359

BLAST of CmoCh20G006280 vs. ExPASy TrEMBL
Match: A0A6J1FYH4 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448677 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.2e-173
Identity = 304/354 (85.88%), Postives = 319/354 (90.11%), Query Frame = 0

Query: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60
           ME K+DELLQSQAHVWNH LK F+SMSL+CAVELGIPD IH+HAQPMS         IQP
Sbjct: 1   MEEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMS---------IQP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 120
           SKAQHLGRLMRLLVHSGFFDA+EQEDV+YSLTPSSR LLRHT THT TTFQI PFLFLSL
Sbjct: 61  SKAQHLGRLMRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSL 120

Query: 121 DKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI 180
           DK TIAS+GALSGWLC S+ DHN S FEMANGKP+W+Y+AQEPS  NLF Q M CDSE I
Sbjct: 121 DKATIASMGALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFI 180

Query: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 240
           GKIV EKCS VFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSN QP  +NL
Sbjct: 181 GKIVTEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNL 240

Query: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV 300
           SFIGGDMLESKIPPANAVMLKW+LHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVL 
Sbjct: 241 SFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLD 300

Query: 301 NEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLR 355
           NEVEDR+STETQLFFDVLMM NLNGKERNE+EWKNLFMEAGFSDYTIVSKLGLR
Sbjct: 301 NEVEDRESTETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLR 345

BLAST of CmoCh20G006280 vs. ExPASy TrEMBL
Match: A0A0A0LMQ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G415520 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.9e-144
Identity = 266/371 (71.70%), Postives = 295/371 (79.51%), Query Frame = 0

Query: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60
           M   L+EL+QSQAHVWNH  K  NSMSLKC VELGIPD IHSH QPMS   LVAALHI+P
Sbjct: 1   MGENLNELVQSQAHVWNHACKFINSMSLKCVVELGIPDIIHSHGQPMSFPSLVAALHIEP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDA------DEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITP 120
           +KAQ L RLM LLVHSGFF A      D+ EDV+YSLTPSS+LLL ++        Q TP
Sbjct: 61  TKAQCLSRLMNLLVHSGFFTAAQTEAHDKAEDVKYSLTPSSKLLLHNS--------QATP 120

Query: 121 FLFLSLDKTTIASLGALSGWLCRSEDD--HNPSVFEMANGKPVWEYAAQEPSLANLFQQT 180
           FLFLSLDK TIAS   LS W C S ++  H  + FEMANGK +W+YAAQE S ANLFQQT
Sbjct: 121 FLFLSLDKATIASFQNLSSWFCSSNNNGQHYSNAFEMANGKLIWDYAAQEQSFANLFQQT 180

Query: 181 MACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSN 240
           M CDSE IGKIV E+CS VFEGLKSLVDVGGGTG M  AI EAFPHITC+VFDLPQV+SN
Sbjct: 181 MVCDSETIGKIVKEECSEVFEGLKSLVDVGGGTGAMGKAIVEAFPHITCTVFDLPQVISN 240

Query: 241 LQP--NVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGG 300
            QP  N +NL F+ GDM E  IPPANAV+LKW+LHNW+DEQSI+ILKKC+DAIPSR EGG
Sbjct: 241 -QPLQNAKNLRFVEGDMFEEIIPPANAVLLKWILHNWNDEQSIRILKKCRDAIPSRDEGG 300

Query: 301 KLIIIDMVLVNEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSK 360
           KLIIIDMV+  + ED +STETQL FD+LMMVNLNGKERNE EWKNLFMEAGFS Y I+SK
Sbjct: 301 KLIIIDMVMERKKEDIESTETQLLFDLLMMVNLNGKERNENEWKNLFMEAGFSGYKIISK 360

Query: 361 LGLRSLIEVYP 362
           LGLRSLIEVYP
Sbjct: 361 LGLRSLIEVYP 362

BLAST of CmoCh20G006280 vs. NCBI nr
Match: KAG6570887.1 (putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 721.1 bits (1860), Expect = 4.8e-204
Identity = 355/361 (98.34%), Postives = 360/361 (99.72%), Query Frame = 0

Query: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60
           MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP
Sbjct: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 120
           SKAQHLGRLMRLLVHSGFFDA+E+EDV+YSLTPSSRLL+RHTDTHTHTTFQITPFLFLSL
Sbjct: 61  SKAQHLGRLMRLLVHSGFFDANEEEDVKYSLTPSSRLLVRHTDTHTHTTFQITPFLFLSL 120

Query: 121 DKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI 180
           DKTTIASLGALSGWLC SEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI
Sbjct: 121 DKTTIASLGALSGWLCSSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI 180

Query: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 240
           GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNV+NL
Sbjct: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVKNL 240

Query: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV 300
           SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV
Sbjct: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV 300

Query: 301 NEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY 360
           NEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY
Sbjct: 301 NEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY 360

Query: 361 P 362
           P
Sbjct: 361 P 361

BLAST of CmoCh20G006280 vs. NCBI nr
Match: XP_023512501.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 697.2 bits (1798), Expect = 7.4e-197
Identity = 342/361 (94.74%), Postives = 352/361 (97.51%), Query Frame = 0

Query: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60
           MEGKLD LLQSQAHVWNHVLK FNSMSLKCAVELGIPD +HSH QPMSLSDLVAALHIQP
Sbjct: 1   MEGKLDGLLQSQAHVWNHVLKFFNSMSLKCAVELGIPDVMHSHGQPMSLSDLVAALHIQP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 120
           SKAQ+LGRLMRLLVHSGFFDA+E+EDV+YSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL
Sbjct: 61  SKAQYLGRLMRLLVHSGFFDANEEEDVKYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 120

Query: 121 DKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI 180
           DKTTIASLGALSGWLC S+ DHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEM 
Sbjct: 121 DKTTIASLGALSGWLCSSDADHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMT 180

Query: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 240
           GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSN+QPNV+NL
Sbjct: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNIQPNVKNL 240

Query: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV 300
           SFI GDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLII+DMVL 
Sbjct: 241 SFIAGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIILDMVLD 300

Query: 301 NEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY 360
           NEVEDR+STETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY
Sbjct: 301 NEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY 360

Query: 361 P 362
           P
Sbjct: 361 P 361

BLAST of CmoCh20G006280 vs. NCBI nr
Match: XP_023512509.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 686.8 bits (1771), Expect = 1.0e-193
Identity = 341/361 (94.46%), Postives = 349/361 (96.68%), Query Frame = 0

Query: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60
           MEGKLDELLQSQAHVWNHVLK FNSMSLKCAVELGIPD IHSHAQPMSLSDLVAALHIQP
Sbjct: 1   MEGKLDELLQSQAHVWNHVLKFFNSMSLKCAVELGIPDVIHSHAQPMSLSDLVAALHIQP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 120
           SKAQHLGRLMRLLVHSGFFDA+EQEDV+YSLTPSSRLLLRH     +TTFQITPFLFLSL
Sbjct: 61  SKAQHLGRLMRLLVHSGFFDANEQEDVKYSLTPSSRLLLRH-----NTTFQITPFLFLSL 120

Query: 121 DKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI 180
           DKTTIASLGALSGWLC S+ DHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEM 
Sbjct: 121 DKTTIASLGALSGWLCSSDADHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMT 180

Query: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 240
           GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSN+QPNV+NL
Sbjct: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNIQPNVKNL 240

Query: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV 300
           SFI GDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLII+DMVL 
Sbjct: 241 SFIAGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIILDMVLD 300

Query: 301 NEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY 360
           NEVEDR+STETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY
Sbjct: 301 NEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY 356

Query: 361 P 362
           P
Sbjct: 361 P 356

BLAST of CmoCh20G006280 vs. NCBI nr
Match: XP_022943692.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 678.7 bits (1750), Expect = 2.7e-191
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 26  MSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHLGRLMRLLVHSGFFDADEQE 85
           MSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHLGRLMRLLVHSGFFDADEQE
Sbjct: 1   MSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQPSKAQHLGRLMRLLVHSGFFDADEQE 60

Query: 86  DVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRSEDDHNPS 145
           DVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRSEDDHNPS
Sbjct: 61  DVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRSEDDHNPS 120

Query: 146 VFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGT 205
           VFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGT
Sbjct: 121 VFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGT 180

Query: 206 GVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLH 265
           GVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLH
Sbjct: 181 GVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLH 240

Query: 266 NWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRDSTETQLFFDVLMMVNLNG 325
           NWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRDSTETQLFFDVLMMVNLNG
Sbjct: 241 NWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRDSTETQLFFDVLMMVNLNG 300

Query: 326 KERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVYP 362
           KERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVYP
Sbjct: 301 KERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVYP 336

BLAST of CmoCh20G006280 vs. NCBI nr
Match: KAG6570911.1 (putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 656.8 bits (1693), Expect = 1.1e-184
Identity = 320/361 (88.64%), Postives = 336/361 (93.07%), Query Frame = 0

Query: 1   MEGKLDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQP 60
           ME K+DE+LQSQAHVWNH LK F+SMSL+CAVELGIPD IH+HAQPMSLSDLV ALHIQP
Sbjct: 1   MEEKVDEVLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMSLSDLVTALHIQP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSL 120
           SKAQHLGRLMRLLVHSGFFDA+EQEDV+YSLTPSSR LLRHT THT TTFQI PFLFLSL
Sbjct: 61  SKAQHLGRLMRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSL 120

Query: 121 DKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMI 180
           DK TIAS+GALSGWLC S+ DHN S FEMANGKP+W+Y+AQEPS  NLF Q M CDSE I
Sbjct: 121 DKATIASMGALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFI 180

Query: 181 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 240
           GKIV EKCS VFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNV+NL
Sbjct: 181 GKIVTEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVKNL 240

Query: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLV 300
           SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVL 
Sbjct: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLD 300

Query: 301 NEVEDRDSTETQLFFDVLMMVNLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEVY 360
           NEVEDR+STETQLFFDVLMM NLNGKERNE+EWKNLFMEAGFSDYTIV KLGLRSLIE+Y
Sbjct: 301 NEVEDRESTETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVCKLGLRSLIELY 360

Query: 361 P 362
           P
Sbjct: 361 P 361

BLAST of CmoCh20G006280 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 211.8 bits (538), Expect = 8.8e-55
Identity = 131/372 (35.22%), Postives = 202/372 (54.30%), Query Frame = 0

Query: 5   LDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSH--AQPMSLSDLVAALHIQPSK 64
           LDE  ++   +W +V    +  + KCA++L IP+AI +H  +QP++L++L +A+   PS 
Sbjct: 23  LDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPS- 82

Query: 65  AQHLGRLMRLLVHSGFFDADEQED---VRYSLTP-SSRLLLRHTDTHTHTTFQITPFLFL 124
             HL R+MR LVH G F     +D     Y  TP S RL++   D  +     + PF+  
Sbjct: 83  --HLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKS-----LAPFVLF 142

Query: 125 SLDKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSE 184
                 +A    LS  +    +   P  F+  +GK VW +A   P L+++  + MACD+ 
Sbjct: 143 ETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR 202

Query: 185 MIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQV--VSNLQPN 244
            +   V   C G+F+G+ ++VDVGGGTG     + + FP I    FDLP V  V+ +   
Sbjct: 203 RVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDG 262

Query: 245 VRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIID 304
           V N   + GDM +S IP  +A+ +KWVLH+W D+  IKILK CK+A+P     GK++I++
Sbjct: 263 VEN---VEGDMFDS-IPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI--GKVLIVE 322

Query: 305 MVLVNE-----VEDRDS--TETQLFFDVLMMVNLN-GKERNEKEWKNLFMEAGFSDYTIV 361
            V+        V++RD      +L  D++MM + + GKER  KEW  +  EAGF+ Y + 
Sbjct: 323 SVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVR 380

BLAST of CmoCh20G006280 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 181.8 bits (460), Expect = 9.8e-46
Identity = 119/369 (32.25%), Postives = 183/369 (49.59%), Query Frame = 0

Query: 5   LDELLQSQAHVWNHVLKIFNSMSLKCAVELGIPDAIHSH--AQPMSLSDLVAALHIQPSK 64
           LDE  ++   +W +V    +  + KCA++L IP+AI +H  +QP++LS+L +A+   PS 
Sbjct: 9   LDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPS- 68

Query: 65  AQHLGRLMRLLVHSGFFDADEQED---VRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLS 124
             HL R+MR LVH G F     +D     Y+ TP SR ++                    
Sbjct: 69  --HLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM-------------------- 128

Query: 125 LDKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEM 184
                                          +GK +W +A      + L  + MACD+  
Sbjct: 129 ---------------------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDARR 188

Query: 185 IGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRN 244
           +   V   C G+F+G+ ++VDVGGGTG     + + FP I    FDLP V+   Q  +  
Sbjct: 189 VVPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQV-LDG 248

Query: 245 LSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMV- 304
           +  + GDM +S IP ++AV++KWVLH+W D+  IKILK CK+A+      GK++I++ V 
Sbjct: 249 VENVEGDMFDS-IPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNI--GKVLIVECVI 308

Query: 305 ------LVNEVEDRDSTETQLFFDVLMMVNLN-GKERNEKEWKNLFMEAGFSDYTIVSKL 361
                 ++ E  D      +L  D++MMV+ + GKER  KEW  +  EAGF+ Y +    
Sbjct: 309 GEKKNTMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFD 323

BLAST of CmoCh20G006280 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 160.2 bits (404), Expect = 3.1e-39
Identity = 103/328 (31.40%), Postives = 170/328 (51.83%), Query Frame = 0

Query: 42  SHAQPMSLSDLVAALHIQPSKAQHLGRLMRLLVHSGFF------DADEQEDVRYSLTPSS 101
           S+  P+ L+ + A  +  P     + RL+R LV           D + +E   Y L    
Sbjct: 52  SYLSPVDLASMAAPKN--PHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVG 111

Query: 102 RLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPV 161
           + L++  D      F I P++     K    + G +  +L  +  +   S +E AN   +
Sbjct: 112 KKLIKDED-----GFSIAPYVLAGCTK----AKGGVWSYLTEAIQEGGASAWERANEALI 171

Query: 162 WEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEA 221
           +EY  +  +L  +F ++M   + ++ K ++E   G FEG+   VDVGG  G     I   
Sbjct: 172 FEYMKKNENLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSK 231

Query: 222 FPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKI 281
           +PHI    FDLP +V    P +  +  IGGDM + +IP    +++KW+LH+W+DE+ ++I
Sbjct: 232 YPHIKGINFDLPHIVKE-APQIHGVEHIGGDMFD-EIPRGEVILMKWILHDWNDEKCVEI 291

Query: 282 LKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRD-STETQLFFDVLMM-VNLNGKERNEKEW 341
           LK CK A+P   E G++I+I+M++  EV + D +T+  L  D+ MM +   GKER +KE+
Sbjct: 292 LKNCKKALP---ETGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEF 351

Query: 342 KNLFMEAGFSDYTIVSKLGLRSLIEVYP 362
           ++L  EAGF    I+       +IE+YP
Sbjct: 352 EDLAKEAGFKLPKIIYGAYSYWIIELYP 362

BLAST of CmoCh20G006280 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 154.8 bits (390), Expect = 1.3e-37
Identity = 102/328 (31.10%), Postives = 169/328 (51.52%), Query Frame = 0

Query: 42  SHAQPMSLSDLVAALHIQPSKAQHLGRLMRLLVHSGFF------DADEQEDVRYSLTPSS 101
           S+  P+ L+ + A  +  P     + RL+R LV           D + +E   Y L    
Sbjct: 52  SYLSPVDLASMAAPKN--PHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVG 111

Query: 102 RLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRSEDDHNPSVFEMANGKPV 161
           + L++  D      F I P++     K    + G +   +  +  +   S +E AN   +
Sbjct: 112 KKLIKDED-----GFSIAPYVLAGCTK----AKGGVWYNVQHAIQEGGASAWERANEALI 171

Query: 162 WEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEA 221
           +EY  +  +L  +F ++M   + ++ K ++E   G FEG+   VDVGG  G     I   
Sbjct: 172 FEYMKKNENLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSK 231

Query: 222 FPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKI 281
           +PHI    FDLP +V    P +  +  IGGDM + +IP    +++KW+LH+W+DE+ ++I
Sbjct: 232 YPHIKGINFDLPHIVKE-APQIHGVEHIGGDMFD-EIPRGEVILMKWILHDWNDEKCVEI 291

Query: 282 LKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRD-STETQLFFDVLMM-VNLNGKERNEKEW 341
           LK CK A+P   E G++I+I+M++  EV + D +T+  L  D+ MM +   GKER +KE+
Sbjct: 292 LKNCKKALP---ETGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEF 351

Query: 342 KNLFMEAGFSDYTIVSKLGLRSLIEVYP 362
           ++L  EAGF    I+       +IE+YP
Sbjct: 352 EDLAKEAGFKLPKIIYGAYSYWIIELYP 362

BLAST of CmoCh20G006280 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 144.4 bits (363), Expect = 1.7e-34
Identity = 103/342 (30.12%), Postives = 166/342 (48.54%), Query Frame = 0

Query: 26  MSLKCAVELGIPDAIHSHAQPMSLSDLVAALHIQ-PSKAQHLGRLMRLLVHSGFFDADEQ 85
           M+LK A+EL + + +  +  PMS +++ + L  + P     L R++RLL          +
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNR 93

Query: 86  E------DVRYSLTPSSRLLLRHTDTHTHTTFQITPFLFLSLDKTTIASLGALSGWLCRS 145
           +      +  Y L P  + L ++ D        I     ++ DK  + S   L   +   
Sbjct: 94  KLSGDGVERIYGLGPVCKYLTKNED-----GVSIAALCLMNQDKVLMESWYHLKDAIL-- 153

Query: 146 EDDHNPSVFEMANGKPVWEYAAQEPSLANLFQQTMACDSEMIGKIVIEKCSGVFEGLKSL 205
            D   P  F  A G   +EY   +P    +F   M+  S +  K ++E   G FEGL SL
Sbjct: 154 -DGGIP--FNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSL 213

Query: 206 VDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAV 265
           VDVGGG G     I   +P++    FDLP V+ +  P+   +  +GGDM  S +P  +A+
Sbjct: 214 VDVGGGIGATLKMIVSKYPNLKGINFDLPHVIED-APSHPGIEHVGGDMFVS-VPKGDAI 273

Query: 266 MLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLVNEVEDRDSTETQLFFDVL 325
            +KW+ H+W DE  +K LK C +++P   E GK+I+ + +L    +   ST+  +  D +
Sbjct: 274 FMKWICHDWSDEHCVKFLKNCYESLP---EDGKVILAECILPETPDSSLSTKQVVHVDCI 333

Query: 326 MMV-NLNGKERNEKEWKNLFMEAGFSDYTIVSKLGLRSLIEV 360
           M+  N  GKER EKE++ L   +GF    +V      +LIE+
Sbjct: 334 MLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIEL 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B0ZB573.3e-9951.51Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1[more]
B6VJS41.6e-9649.58Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1... [more]
A0A0N9HMN66.3e-9048.07Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P... [more]
Q6VMW01.7e-8746.748-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=... [more]
Q8GSN15.2e-8446.85Myricetin O-methyltransferase OS=Catharanthus roseus OX=4058 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FTR01.3e-191100.00probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448368 PE=... [more]
A0A6J1JFR97.1e-18588.64probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111484102 PE=3 ... [more]
A0A6J1FXQ61.5e-18288.64probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448676 PE=... [more]
A0A6J1FYH41.2e-17385.88probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448677 PE=... [more]
A0A0A0LMQ97.9e-14471.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G415520 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6570887.14.8e-20498.34putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023512501.17.4e-19794.74probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo][more]
XP_023512509.11.0e-19394.46probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo][more]
XP_022943692.12.7e-191100.00probable O-methyltransferase 3 [Cucurbita moschata][more]
KAG6570911.11.1e-18488.64putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT4G35160.18.8e-5535.22O-methyltransferase family protein [more]
AT4G35150.19.8e-4632.25O-methyltransferase family protein [more]
AT1G51990.13.1e-3931.40O-methyltransferase family protein [more]
AT1G51990.21.3e-3731.10O-methyltransferase family protein [more]
AT5G54160.11.7e-3430.12O-methyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 2..98
e-value: 6.4E-40
score: 136.9
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 26..74
e-value: 6.0E-18
score: 64.6
IPR016461O-methyltransferase COMT-typePIRSFPIRSF005739O-mtasecoord: 1..361
e-value: 1.8E-120
score: 400.1
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 6..361
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 16..361
score: 77.327911
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 106..361
e-value: 8.7E-78
score: 262.9
NoneNo IPR availablePIRSRPIRSR005739-1PIRSR005739-1coord: 25..360
e-value: 1.6E-85
score: 284.6
NoneNo IPR availablePANTHERPTHR11746:SF250O-METHYLTRANSFERASEcoord: 6..361
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 198..299
e-value: 0.0013266
score: 35.8687
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 142..343
e-value: 3.5E-56
score: 189.9
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 112..360
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 6..101

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G006280.1CmoCh20G006280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019438 aromatic compound biosynthetic process
biological_process GO:0032259 methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity