CmoCh20G005830 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G005830
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionindole-3-acetic acid-amido synthetase GH3.10
LocationCmo_Chr20: 2871871 .. 2875365 (+)
RNA-Seq ExpressionCmoCh20G005830
SyntenyCmoCh20G005830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGTGCTGCTGTTGCGGCTGCTGCGGCTGCGGGCGTGTTCAAGGCGGCGAGGTGCGCACCATGTTTGAAGACGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAATTGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAATCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACACAACAACCCATTCGAACTCTCTCTTTAAGGTACACCCTTCTTCAACATTTTCCCCTACATTTTATGAATTCCCTCTTTATTACAAATGATATCATTACGCCGCTGCAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGTACCAATGCAATATGGTCAGAATCTTCATTTTTTTTTAGTTGCCCTTTTCTTGCAACACATTAAAGAATGCCTTAATGACAACTAGGGTTTATCCCACAAGGGAGGGAGGCAGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACGGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTTCTATTGGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCTTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCATGCCACACTCTGCTCTAGAATCACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCAAACCCAATTCTTGGGGCGAAAATTGGAGCTGTTTGTGAAGAGCTTGAGAGACAGGATTGGTTGGATTTGGTTCCGAAGCTATGGCCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGCTATGCGGGGAGGTTGCCGTTGGTGAGTGGGGATTATGGATCGACAGAGAGTTGGATTGGGGTTAATGTTGATCCTTGTCTGCCGCCTGAGAATGTTACATTTGCGGTCGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGCCTTCGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGTTAGTTCATTAATTATCAACACAAATCTAATCAAACGCCTTGCTTTTGCTCCTTCCTTTATCATTATGTCATCCATAACTATTGCAAAATATCTATTCCATGGAGAAGATTAGTTTTTAAAACGCGTTTAATGGGGAGAGTTGTTTCGTGTGATCACCCAATTCGCTTCTCTTGGAGACCCACACATAGTTCAATGTCTAGTTCTTAAACGAACTGTAACGACCCGACTTTTCTTTGTAATGACTCTGACCGCTATGTATAAGAAAAGTCGAGCCGTTACAATTAGTATCATGATTTGGTAATCATGGATCATTGATTTGATCAGTAATGATCAGTCCTATACGGACAAGTTGAGAGAAATTTCCAACCTTCCTTATTTGGCTCAAAAATTTCCGAACTTTCCTTGTCAACATGATGCTTACATTGATTTCGCAAAGGTAAAATTTTGTACCAAGTTTTGTTTTTTTATTGAAATTTTCAATAAAGCCTAAAAAATGTGAAAACTTTTTTTACTACAGTTTACCTTCACATCAAATATAGAGCATCCGAGAGTAAACATGTAACCATTCAAGCCCATTACTACCTGATATTATCCTGTTTAGACTTTCTGTCTCGAGCTTCTCTTAAAATGCATCTTTAGACTTTCCCTTTCGAGCTTCCCCTCATTTAATGGTAAATATGTGAACATTCTTTACTAAATTTTTCTTGTCACATCAAATATAGAGCATATAGATCATATGATTAATATATAATAGCTCGAGTATTATATTCTTTGTGTTTTCCTTTACGATCTTCTCTTTAAGGTTTTTAAGACACGTCTGTTGAGAATAGGTTTTTACGTACTAGTTATAAGGAGTAGTGTTTCGTTCTCATCTTTGAAAGAGGTGGGATTTCACCGAATATATTGACATAGATCAATTTAAACATAGCTCAGATGATTATGACATATATGTTTCATCTTCGACCCAAAGGGGTTAATGGGTTTTGGTATTGGTTGCTCAACGGATGACTATGACATGTATCTTCGATCGAAAGGGGTTAATGGGTTTTGGGTATTGGTTGTGATTGGCAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTAGTGGAACGAGGGTCCCAGCTGCTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGGCACGTCGAGGACGCAGTTCTTGGACACTGCTGCTCGGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTGTCCAGGCGGTCCAACACCATCGGACCCCTCGAGCTAAGGATCGTGGAAAGAGGCAGCTTCAACAAGATCATGGAACATTACATTGGTAACGGAGCTGCACTTAGCCAATTCAAGACCCCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACTGTCAAAGCCTTTCATAGCACTGCTTATGGCTAAGTTTGGTTATTAATATTGTTAGTATTTCCCAATTTTGAATCAGTTTCGGATCATTTCATTCTAACCAAATTAGAGTGTCACTGAACACTTACTCATCATTGCATGTAACGAGCCGAAACGAATAATATTTGTTAGTGGTGGGTTTCGGTTGTTATAATCAGTATCAGAGATAGACACCGAGCTGTCGTATCAGTTGGAGAATAGAACAAATCATTCCTTATAGATGCTATCATAGTACCTCGAGAGTGAATAAAGTTTCGGTCGTCGTCGTCGTCTTGTTGTTTTTGCTTTTATCATCATAAAGCTGCCTCAACTCTTCTTCCACTGCTAGCTCTTAATGGGTTTGCTTTTGATTTCTACTAAAATCCTCCATTTGTGCATTATTTACTGCTACCCCCCTCCCCTATTCCTTCT

mRNA sequence

TCGTGCTGCTGTTGCGGCTGCTGCGGCTGCGGGCGTGTTCAAGGCGGCGAGGTGCGCACCATGTTTGAAGACGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAATTGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAATCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACACAACAACCCATTCGAACTCTCTCTTTAAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGGTTTATCCCACAAGGGAGGGAGGCAGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACGGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTTCTATTGGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCTTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCATGCCACACTCTGCTCTAGAATCACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCAAACCCAATTCTTGGGGCGAAAATTGGAGCTGTTTGTGAAGAGCTTGAGAGACAGGATTGGTTGGATTTGGTTCCGAAGCTATGGCCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGCTATGCGGGGAGGTTGCCGTTGGTGAGTGGGGATTATGGATCGACAGAGAGTTGGATTGGGGTTAATGTTGATCCTTGTCTGCCGCCTGAGAATGTTACATTTGCGGTCGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGCCTTCGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTAGTGGAACGAGGGTCCCAGCTGCTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGGCACGTCGAGGACGCAGTTCTTGGACACTGCTGCTCGGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTGTCCAGGCGGTCCAACACCATCGGACCCCTCGAGCTAAGGATCGTGGAAAGAGGCAGCTTCAACAAGATCATGGAACATTACATTGGTAACGGAGCTGCACTTAGCCAATTCAAGACCCCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACTGTCAAAGCCTTTCATAGCACTGCTTATGGCTAAGTTTGGTTATTAATATTGTTAGTATTTCCCAATTTTGAATCAGTTTCGGATCATTTCATTCTAACCAAATTAGAGTGTCACTGAACACTTACTCATCATTGCATGTAACGAGCCGAAACGAATAATATTTGTTAGTGGTGGGTTTCGGTTGTTATAATCAGTATCAGAGATAGACACCGAGCTGTCGTATCAGTTGGAGAATAGAACAAATCATTCCTTATAGATGCTATCATAGTACCTCGAGAGTGAATAAAGTTTCGGTCGTCGTCGTCGTCTTGTTGTTTTTGCTTTTATCATCATAAAGCTGCCTCAACTCTTCTTCCACTGCTAGCTCTTAATGGGTTTGCTTTTGATTTCTACTAAAATCCTCCATTTGTGCATTATTTACTGCTACCCCCCTCCCCTATTCCTTCT

Coding sequence (CDS)

ATGTTTGAAGACGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAATTGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAATCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACACAACAACCCATTCGAACTCTCTCTTTAAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGGTTTATCCCACAAGGGAGGGAGGCAGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACGGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTTCTATTGGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCTTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCATGCCACACTCTGCTCTAGAATCACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCAAACCCAATTCTTGGGGCGAAAATTGGAGCTGTTTGTGAAGAGCTTGAGAGACAGGATTGGTTGGATTTGGTTCCGAAGCTATGGCCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGCTATGCGGGGAGGTTGCCGTTGGTGAGTGGGGATTATGGATCGACAGAGAGTTGGATTGGGGTTAATGTTGATCCTTGTCTGCCGCCTGAGAATGTTACATTTGCGGTCGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGCCTTCGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTAGTGGAACGAGGGTCCCAGCTGCTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGGCACGTCGAGGACGCAGTTCTTGGACACTGCTGCTCGGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTGTCCAGGCGGTCCAACACCATCGGACCCCTCGAGCTAAGGATCGTGGAAAGAGGCAGCTTCAACAAGATCATGGAACATTACATTGGTAACGGAGCTGCACTTAGCCAATTCAAGACCCCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACTGTCAAAGCCTTTCATAGCACTGCTTATGGCTAA

Protein sequence

MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Homology
BLAST of CmoCh20G005830 vs. ExPASy Swiss-Prot
Match: Q9ZNS2 (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 6.2e-236
Identity = 396/588 (67.35%), Postives = 468/588 (79.59%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           +D++ WFE VS  A   Q  TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +P+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185
           FR++AAYRSR YP REGGRILEFIY+ K+ KT GG+T GTATTHYYASE+FK KQ  TKS
Sbjct: 129 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 188

Query: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245
           FTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQAF+  E+ W +IC D
Sbjct: 189 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 248

Query: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQ-DWLDLVPKLWPNCKYV 305
           I    L SRITLP++RKAVL +I PNP L + I  +C ELE    W  L+ KLWPN K++
Sbjct: 249 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 308

Query: 306 YSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYF 365
            SIMTGSM PYL KLR YAG LPLVS DYGSTESWIGVNVDP LPPE+V+FAV+PTFSYF
Sbjct: 309 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 368

Query: 366 EFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDV 425
           EFIPL+R+  Q D     +    DF+E +P+PLSQVK+GQ+YELVLTTFTGLYR RLGDV
Sbjct: 369 EFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDV 428

Query: 426 VEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 485
           VEV  FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQLL+  + AE+VDFTSH
Sbjct: 429 VEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSH 488

Query: 486 ADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVER 545
           AD+  +PGHYVI+WE++G  +D  L  CC EMD AFVDYGYVVSRR N+IGPLELR+VER
Sbjct: 489 ADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVER 548

Query: 546 GSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           G+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S+AY
Sbjct: 549 GTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

BLAST of CmoCh20G005830 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 4.6e-170
Identity = 295/591 (49.92%), Postives = 409/591 (69.20%), Query Frame = 0

Query: 4   DVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFT 63
           D E++++ FE ++ +AG  Q  TL++IL +N G EYL QW     ++   D  +    F 
Sbjct: 12  DPEEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQW----GLNGKTDSLS----FK 71

Query: 64  SLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTL 123
           + IP+ +H D EP++ RIADGD SP+LT +PI T+SLSSGTT+G+ K+VPF     ++T+
Sbjct: 72  NCIPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTM 131

Query: 124 LIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKT 183
            IF+ +  +R+R +P    G+ L+FIY SKQ KTKGG+  GTATT+ Y + QFK      
Sbjct: 132 QIFKTSFVFRNREFPV-VNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAM 191

Query: 184 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQIC 243
           ++  CSP EVI G D++QS YCHLL GL F D+V+ V+STFA++IV AF   E  W ++ 
Sbjct: 192 QTPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELV 251

Query: 244 HDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKY 303
            +I    L SR+ +P +R A+  ++ P+P L   I   C  L   +W  L+P+L+PN +Y
Sbjct: 252 TNIREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRL--SNWYGLIPELFPNTRY 311

Query: 304 VYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSY 363
           +Y IMTGSM+PYLKKLR YAG LPL+S DYGS+E WIG NV+P LPPE VT+AV+P   Y
Sbjct: 312 IYGIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGY 371

Query: 364 FEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 423
           FEFIPL                  D LE  P+ L++VK+G++YE+V+T F GLYR RLGD
Sbjct: 372 FEFIPLMENL--------------DGLEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGD 431

Query: 424 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 483
           VV++ GFHN TP+L FICRR L+L++NIDKNTEKDLQL VE  +++L+D+   E+VDFTS
Sbjct: 432 VVKIKGFHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEK-LEVVDFTS 491

Query: 484 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 543
           H +++  PGHYVIFWE+ G   + +L  CC+ +D +FVD GYV SR+ + IG LELRIV+
Sbjct: 492 HVNVSADPGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVK 551

Query: 544 RGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
           RG+F+KI++H++G GAA+SQFKTPRC   TN  +L IL+ + V+++ STA+
Sbjct: 552 RGTFHKILDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFSTAF 576

BLAST of CmoCh20G005830 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 593.2 bits (1528), Expect = 3.3e-168
Identity = 298/591 (50.42%), Postives = 400/591 (67.68%), Query Frame = 0

Query: 4   DVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFT 63
           D E +++ FE ++ +AG  Q  TL++IL QN G EYL  W   +N    P        F 
Sbjct: 13  DQEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLW--GLNGRTDP------QTFK 72

Query: 64  SLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTL 123
           + +P+ +H D EP++QRIADGD SP+LT +PI T+SLSSGTT+G+ K+VPF     ++T+
Sbjct: 73  NCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMESTM 132

Query: 124 LIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKT 183
            IF+ + A+R+R +P    G+ L+FIYSSKQ KTKGG+  GTATT+ Y + QFK      
Sbjct: 133 QIFKTSFAFRNREFPI-GNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAM 192

Query: 184 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQIC 243
            +  CSP EVI G D+ QS YCHLL GL F D+V+ V+STFA++IV AF   E  W  + 
Sbjct: 193 CTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALV 252

Query: 244 HDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKY 303
            DI    L SR+T+P IR A+  ++ P+P L   I   C  L   +W  L+P L+PN +Y
Sbjct: 253 VDIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRL--SNWYGLIPDLFPNTRY 312

Query: 304 VYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSY 363
           +Y IMTGSM+PYLKKLR YAG LPL+S DYGS+E W+GVNV+P LPPE VT+AV+P   Y
Sbjct: 313 IYGIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGY 372

Query: 364 FEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 423
           FEFIPL       +  +S            P+ L++VK+G++YE+V T F GLYR RLGD
Sbjct: 373 FEFIPLGGNLNGIEQANS------------PVGLTEVKLGEEYEVVFTNFAGLYRYRLGD 432

Query: 424 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 483
           VV+V GFHN TP+L F+CR  L+L++NIDKNTEKDLQL VE  ++ L D+   E+VDFTS
Sbjct: 433 VVKVKGFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEK-LEVVDFTS 492

Query: 484 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 543
           H +++  PGHYVIFWE+ G   D +L  CC+ +D +F+D GYV SR+ N IG LELRIV+
Sbjct: 493 HVNVSADPGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALELRIVK 552

Query: 544 RGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
           RG+F+KI++H++G G A+SQFKTPRC    N  LL IL+ + V+ + STA+
Sbjct: 553 RGTFHKILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVSTAF 579

BLAST of CmoCh20G005830 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 9.2e-163
Identity = 287/591 (48.56%), Postives = 392/591 (66.33%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYL-NQWLGDVNIHQIPDDFALESLFTS 65
           E+ ++ FE ++ +A   Q  TL++IL  N   EYL N  LG     +          + S
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAE---------SYKS 66

Query: 66  LIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLL 125
            IPL  H D EP++QRI DGD+SP++T +PI  LSLSSGTT G+ K++PF     +TTL 
Sbjct: 67  CIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQ 126

Query: 126 IFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTK 185
           I+R + A+R+R YP  + G+ L+F+Y SKQ  TKGGI   TATT+ Y  +++K      +
Sbjct: 127 IYRTSYAFRNREYPIGQ-GKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQ 186

Query: 186 SFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICH 245
           S  CSP EVI G D+ QS YCHLL GL +S++V  V STFA+++V AF   E+ W  +C 
Sbjct: 187 SQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCT 246

Query: 246 DITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYV 305
           DI    L  ++T P IR+AV  I+ PNP L   I   C  L   +W  ++P LWPN KYV
Sbjct: 247 DIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGL--SNWYGVIPALWPNAKYV 306

Query: 306 YSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYF 365
           Y IMTGSM+PYLKKLR YAG LPL+S DYG++E W+G N+DP +PPE VT+AV+P   YF
Sbjct: 307 YGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYF 366

Query: 366 EFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDV 425
           EFIPL +   +    S+S+     ++E  P+ L++V++G+ YE+V+T F GLYR RLGDV
Sbjct: 367 EFIPLEKPIGEETENSASI----HYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDV 426

Query: 426 VEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 485
           V++A FHN TP+L FICRR LVL++NIDKNTEKDLQL VE  S+ L  +   E++DFTS 
Sbjct: 427 VKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEK-LEVMDFTSF 486

Query: 486 ADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVER 545
            + ++ PG YVIFWE+ G   D VL  C + +D AF+D GY  SR+  TIGPLELRI+ +
Sbjct: 487 VERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRK 546

Query: 546 GSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAYG 594
           G+F +I++H++  G A+SQFKTPR    +N  +L IL+ +  +++ STAYG
Sbjct: 547 GTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAYG 580

BLAST of CmoCh20G005830 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 549.3 bits (1414), Expect = 5.4e-155
Identity = 273/591 (46.19%), Postives = 383/591 (64.81%), Query Frame = 0

Query: 4   DVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFT 63
           D+  ++D F+ ++  A   Q +TL+EIL +N    YL       N     +    E  F 
Sbjct: 9   DMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNATDPEEAFK 68

Query: 64  SLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTL 123
           S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT    + TL
Sbjct: 69  SMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTL 128

Query: 124 LIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKT 183
            +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  FK      
Sbjct: 129 QLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSI 188

Query: 184 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQIC 243
            S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   E  W +I 
Sbjct: 189 TSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIV 248

Query: 244 HDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKY 303
            DI    L +RIT+P +R A+  +++PNP L   I   C  L   +W  L+P L+PN KY
Sbjct: 249 TDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKY 308

Query: 304 VYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSY 363
           VY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TFAV+P   Y
Sbjct: 309 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 368

Query: 364 FEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 423
           FEF+P+       + G           E +P+ L+QVKIG++YE+V+T + GLYR RLGD
Sbjct: 369 FEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGD 428

Query: 424 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 483
           VV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++   E++DF+S
Sbjct: 429 VVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSS 488

Query: 484 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 543
           + D++  PGHY IFWE+ G   + VL  CC+ +D AF+D GYV SR+  TIG LELR+V 
Sbjct: 489 YIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVA 548

Query: 544 RGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
           +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 549 KGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of CmoCh20G005830 vs. ExPASy TrEMBL
Match: A0A6J1FRQ1 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LOC111448207 PE=3 SV=1)

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 593/593 (100.00%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmoCh20G005830 vs. ExPASy TrEMBL
Match: A0A6J1J741 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC111484069 PE=3 SV=1)

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 582/593 (98.15%), Postives = 588/593 (99.16%), Query Frame = 0

Query: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEELERQDWLDLVPKLW N
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAG LPLVSGDYGSTESWIG+NVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmoCh20G005830 vs. ExPASy TrEMBL
Match: A0A5D3B9T4 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00300 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 8.9e-294
Identity = 493/590 (83.56%), Postives = 540/590 (91.53%), Query Frame = 0

Query: 3   EDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           E+ ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSLIPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TT
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTT 127

Query: 123 LLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLK 182
           LLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQLK
Sbjct: 128 LLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLK 187

Query: 183 TKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQI 242
           TKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYTIVQAFN+LE+ W +I
Sbjct: 188 TKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEI 247

Query: 243 CHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCK 302
            HDI+HATL SRI +PEIRKAVL ++SP P LG KI  VCEEL+++ WL L+PKLWPNCK
Sbjct: 248 IHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPKLWPNCK 307

Query: 303 YVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFS 362
           YVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP LPPENVTFAV+PTFS
Sbjct: 308 YVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFS 367

Query: 363 YFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLG 422
           YFEFIPLF+QH      SS+  A  DFLEGQP+ LS+VKIGQQYELVLTTFTGLYRCRLG
Sbjct: 368 YFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLG 427

Query: 423 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFT 482
           DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGSQL++  SGAELVDFT
Sbjct: 428 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFT 487

Query: 483 SHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIV 542
           S+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVVSR++N+IGPLELRIV
Sbjct: 488 SYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 547

Query: 543 ERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           ERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F STAY
Sbjct: 548 ERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAY 594

BLAST of CmoCh20G005830 vs. ExPASy TrEMBL
Match: A0A1S3BIL6 (jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 8.9e-294
Identity = 493/590 (83.56%), Postives = 540/590 (91.53%), Query Frame = 0

Query: 3   EDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           E+ ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSLIPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TT
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTT 127

Query: 123 LLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLK 182
           LLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQLK
Sbjct: 128 LLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLK 187

Query: 183 TKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQI 242
           TKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYTIVQAFN+LE+ W +I
Sbjct: 188 TKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEI 247

Query: 243 CHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCK 302
            HDI+HATL SRI +PEIRKAVL ++SP P LG KI  VCEEL+++ WL L+PKLWPNCK
Sbjct: 248 IHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPKLWPNCK 307

Query: 303 YVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFS 362
           YVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP LPPENVTFAV+PTFS
Sbjct: 308 YVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFS 367

Query: 363 YFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLG 422
           YFEFIPLF+QH      SS+  A  DFLEGQP+ LS+VKIGQQYELVLTTFTGLYRCRLG
Sbjct: 368 YFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLG 427

Query: 423 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFT 482
           DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGSQL++  SGAELVDFT
Sbjct: 428 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFT 487

Query: 483 SHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIV 542
           S+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVVSR++N+IGPLELRIV
Sbjct: 488 SYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 547

Query: 543 ERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           ERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F STAY
Sbjct: 548 ERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAY 594

BLAST of CmoCh20G005830 vs. ExPASy TrEMBL
Match: A0A0A0KE57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=3 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.5e-290
Identity = 487/590 (82.54%), Postives = 535/590 (90.68%), Query Frame = 0

Query: 3   EDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           E  ED++ WFE+VSSEA +AQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L S+F
Sbjct: 2   EGEEDIIGWFEQVSSEAALAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLHSIF 61

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TS IPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTT
Sbjct: 62  TSSIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAQTT 121

Query: 123 LLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLK 182
           LLIFR+AAAYRSRVYP R+GG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQLK
Sbjct: 122 LLIFRLAAAYRSRVYPIRDGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLK 181

Query: 183 TKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQI 242
           TKSFTCSPQEVI+G DYKQSTYCHLLLGL +S++VEFVTSTFAYTIVQAFN+LE+ W ++
Sbjct: 182 TKSFTCSPQEVITGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEM 241

Query: 243 CHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCK 302
            HD++HATL SRI +PEIRKAVL ++SP P LG KI  VCEEL ++ WL L+PKLWPNCK
Sbjct: 242 IHDLSHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELMKEGWLGLIPKLWPNCK 301

Query: 303 YVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFS 362
           YVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP LPPENVTFAV+PTFS
Sbjct: 302 YVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFS 361

Query: 363 YFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLG 422
           YFEFIPLF+QH      SS+  A  DFLEGQP+ LS+VKIGQQYELVLTTFTGLYRCRLG
Sbjct: 362 YFEFIPLFKQHA---TTSSADTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLG 421

Query: 423 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFT 482
           DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGSQL++  SGAELVDFT
Sbjct: 422 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFT 481

Query: 483 SHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIV 542
           S+A+L+NQPGHYVIFWE+KG V D VLG CCSEMDAAFVDYGYVVSR++N+IGPLELRIV
Sbjct: 482 SYAELSNQPGHYVIFWELKGDVNDKVLGMCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 541

Query: 543 ERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           ERGSFNKI+EHYIGNGAALSQFKTPRCTTN  LL ILNLST+K+F STAY
Sbjct: 542 ERGSFNKILEHYIGNGAALSQFKTPRCTTNHFLLNILNLSTLKSFFSTAY 588

BLAST of CmoCh20G005830 vs. NCBI nr
Match: XP_022943441.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata])

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 593/593 (100.00%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmoCh20G005830 vs. NCBI nr
Match: KAG6570848.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 591/593 (99.66%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmoCh20G005830 vs. NCBI nr
Match: XP_023512179.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 589/593 (99.33%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGS+QPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmoCh20G005830 vs. NCBI nr
Match: KAG7010696.1 (Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1201.0 bits (3106), Expect = 0.0e+00
Identity = 589/608 (96.88%), Postives = 591/608 (97.20%), Query Frame = 0

Query: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 594
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600

BLAST of CmoCh20G005830 vs. NCBI nr
Match: XP_022986272.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima])

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 582/593 (98.15%), Postives = 588/593 (99.16%), Query Frame = 0

Query: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEELERQDWLDLVPKLW N
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300

Query: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360
           CKYVYSIMTGSMQPYLKKLRRYAG LPLVSGDYGSTESWIG+NVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360

Query: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480
           LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540
           FTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 594
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of CmoCh20G005830 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 818.1 bits (2112), Expect = 4.4e-237
Identity = 396/588 (67.35%), Postives = 468/588 (79.59%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           +D++ WFE VS  A   Q  TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +P+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185
           FR++AAYRSR YP REGGRILEFIY+ K+ KT GG+T GTATTHYYASE+FK KQ  TKS
Sbjct: 129 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 188

Query: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245
           FTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQAF+  E+ W +IC D
Sbjct: 189 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 248

Query: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQ-DWLDLVPKLWPNCKYV 305
           I    L SRITLP++RKAVL +I PNP L + I  +C ELE    W  L+ KLWPN K++
Sbjct: 249 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 308

Query: 306 YSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYF 365
            SIMTGSM PYL KLR YAG LPLVS DYGSTESWIGVNVDP LPPE+V+FAV+PTFSYF
Sbjct: 309 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 368

Query: 366 EFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDV 425
           EFIPL+R+  Q D     +    DF+E +P+PLSQVK+GQ+YELVLTTFTGLYR RLGDV
Sbjct: 369 EFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDV 428

Query: 426 VEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 485
           VEV  FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQLL+  + AE+VDFTSH
Sbjct: 429 VEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSH 488

Query: 486 ADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVER 545
           AD+  +PGHYVI+WE++G  +D  L  CC EMD AFVDYGYVVSRR N+IGPLELR+VER
Sbjct: 489 ADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVER 548

Query: 546 GSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 593
           G+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S+AY
Sbjct: 549 GTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

BLAST of CmoCh20G005830 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 549.3 bits (1414), Expect = 3.8e-156
Identity = 273/591 (46.19%), Postives = 383/591 (64.81%), Query Frame = 0

Query: 4   DVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFT 63
           D+  ++D F+ ++  A   Q +TL+EIL +N    YL       N     +    E  F 
Sbjct: 9   DMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNATDPEEAFK 68

Query: 64  SLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTL 123
           S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT    + TL
Sbjct: 69  SMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTL 128

Query: 124 LIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKT 183
            +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  FK      
Sbjct: 129 QLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSI 188

Query: 184 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQIC 243
            S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   E  W +I 
Sbjct: 189 TSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIV 248

Query: 244 HDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKY 303
            DI    L +RIT+P +R A+  +++PNP L   I   C  L   +W  L+P L+PN KY
Sbjct: 249 TDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKY 308

Query: 304 VYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSY 363
           VY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TFAV+P   Y
Sbjct: 309 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 368

Query: 364 FEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 423
           FEF+P+       + G           E +P+ L+QVKIG++YE+V+T + GLYR RLGD
Sbjct: 369 FEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGD 428

Query: 424 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 483
           VV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++   E++DF+S
Sbjct: 429 VVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSS 488

Query: 484 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 543
           + D++  PGHY IFWE+ G   + VL  CC+ +D AF+D GYV SR+  TIG LELR+V 
Sbjct: 489 YIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVA 548

Query: 544 RGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
           +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 549 KGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of CmoCh20G005830 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 549.3 bits (1414), Expect = 3.8e-156
Identity = 273/591 (46.19%), Postives = 383/591 (64.81%), Query Frame = 0

Query: 4   DVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFT 63
           D+  ++D F+ ++  A   Q +TL+EIL +N    YL       N     +    E  F 
Sbjct: 9   DMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNATDPEEAFK 68

Query: 64  SLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTL 123
           S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT    + TL
Sbjct: 69  SMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTL 128

Query: 124 LIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKT 183
            +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  FK      
Sbjct: 129 QLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSI 188

Query: 184 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQIC 243
            S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   E  W +I 
Sbjct: 189 TSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIV 248

Query: 244 HDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKY 303
            DI    L +RIT+P +R A+  +++PNP L   I   C  L   +W  L+P L+PN KY
Sbjct: 249 TDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKY 308

Query: 304 VYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSY 363
           VY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TFAV+P   Y
Sbjct: 309 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 368

Query: 364 FEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 423
           FEF+P+       + G           E +P+ L+QVKIG++YE+V+T + GLYR RLGD
Sbjct: 369 FEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGD 428

Query: 424 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 483
           VV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++   E++DF+S
Sbjct: 429 VVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSS 488

Query: 484 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 543
           + D++  PGHY IFWE+ G   + VL  CC+ +D AF+D GYV SR+  TIG LELR+V 
Sbjct: 489 YIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVA 548

Query: 544 RGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
           +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 549 KGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of CmoCh20G005830 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 549.3 bits (1414), Expect = 3.8e-156
Identity = 273/591 (46.19%), Postives = 383/591 (64.81%), Query Frame = 0

Query: 4   DVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFT 63
           D+  ++D F+ ++  A   Q +TL+EIL +N    YL       N     +    E  F 
Sbjct: 20  DMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNATDPEEAFK 79

Query: 64  SLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTL 123
           S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT    + TL
Sbjct: 80  SMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTL 139

Query: 124 LIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKT 183
            +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  FK      
Sbjct: 140 QLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSI 199

Query: 184 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQIC 243
            S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   E  W +I 
Sbjct: 200 TSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIV 259

Query: 244 HDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKY 303
            DI    L +RIT+P +R A+  +++PNP L   I   C  L   +W  L+P L+PN KY
Sbjct: 260 TDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKY 319

Query: 304 VYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSY 363
           VY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TFAV+P   Y
Sbjct: 320 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 379

Query: 364 FEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 423
           FEF+P+       + G           E +P+ L+QVKIG++YE+V+T + GLYR RLGD
Sbjct: 380 FEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGD 439

Query: 424 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 483
           VV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++   E++DF+S
Sbjct: 440 VVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSS 499

Query: 484 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 543
           + D++  PGHY IFWE+ G   + VL  CC+ +D AF+D GYV SR+  TIG LELR+V 
Sbjct: 500 YIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVA 559

Query: 544 RGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
           +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 560 KGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

BLAST of CmoCh20G005830 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 3.6e-146
Identity = 251/514 (48.83%), Postives = 344/514 (66.93%), Query Frame = 0

Query: 81  IADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAAYRSRVYPTR 140
           + DGD+SP+LT  P+  +SLSSGT++GR K++PFT    + TL +FR A A+R+R +P  
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 141 EGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYK 200
           + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +CSP EVI   D  
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 201 QSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEI 260
           Q+ YCHLL G+ F DQV++V + FA+ +V AF   E  W +I  DI    L +RIT+P +
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 261 RKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLR 320
           R A+  +++PNP L   I   C  L   +W  L+P L+PN KYVY IMTGSM+PY+ KLR
Sbjct: 181 RTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLR 240

Query: 321 RYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGS 380
            YAG LPLVS DYGS+E WI  NV P L PE  TFAV+P   YFEF+P+       + G 
Sbjct: 241 HYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPV------SETGE 300

Query: 381 SSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFI 440
                     E +P+ L+QVKIG++YE+V+T + GLYR RLGDVV+V GF+N TP+L FI
Sbjct: 301 G---------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFI 360

Query: 441 CRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEV 500
           CRR L+L++NIDKNTE+DLQL VE  ++ L+++   E++DF+S+ D++  PGHY IFWE+
Sbjct: 361 CRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFWEI 420

Query: 501 KGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAA 560
            G   + VL  CC+ +D AF+D GYV SR+  TIG LELR+V +G+F KI EH++G G++
Sbjct: 421 SGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSS 480

Query: 561 LSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 593
             QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 481 AGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZNS26.2e-23667.35Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=... [more]
A0A1J6KGJ94.6e-17049.92Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ... [more]
A0A314KSQ43.3e-16850.42Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ... [more]
Q6I5819.2e-16348.56Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q9SKE25.4e-15546.19Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FRQ10.0e+00100.00indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1J7410.0e+0098.15indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5D3B9T48.9e-29483.56Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BIL68.9e-29483.56jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KE573.5e-29082.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022943441.10.0e+00100.00indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata][more]
KAG6570848.10.0e+0099.66Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023512179.10.0e+0099.33indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo][more]
KAG7010696.10.0e+0096.88Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argy... [more]
XP_022986272.10.0e+0098.15indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G03400.14.4e-23767.35Auxin-responsive GH3 family protein [more]
AT2G46370.13.8e-15646.19Auxin-responsive GH3 family protein [more]
AT2G46370.23.8e-15646.19Auxin-responsive GH3 family protein [more]
AT2G46370.43.8e-15646.19Auxin-responsive GH3 family protein [more]
AT2G46370.33.6e-14648.83Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 11..572
e-value: 5.8E-174
score: 579.8
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 6..592
NoneNo IPR availablePANTHERPTHR31901:SF48INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.10coord: 6..592

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G005830.1CmoCh20G005830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009416 response to light stimulus
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity