CmoCh20G005560 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G005560
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionE3 ubiquitin-protein ligase RZFP34-like
LocationCmo_Chr20: 2686409 .. 2690191 (-)
RNA-Seq ExpressionCmoCh20G005560
SyntenyCmoCh20G005560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCGCACTGTGCAGATCTCTGTAACGAAAGGATTTTTTTTTCTTTTTCTGAGTGCTGTTTGTTCTTGCTCTTTTTCCGTTTCAATGGAGATTCATATTCAGGCTTCGTTATCTTCGCGTTCATCATCTGAATCTGAAATTTCTGCTTCTGATAATATAGATGCTGCGGATCGTGAATCTGGGAATTTCGGGTATGAATCTGCTCTGCTAAACTTCTGTTTTGTAAATGTCGATCTGCGTTTCGTTTTCGCTTTGGATTTTGTATCTCGTTCCTTAACTATCGATTGTTCTGGTCTAGGTGCTCGCATTATCGAAGGAGATGCAAAATTAGAACGCCCTGTTGCGATGAGATCTTTGATTGTAGGCATTGTCACAATGAAGCGAAGGTTGAAATTCTATTAAACTATTTCCTGTTTCAGCCTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTGGTAATTGATTTTGGTGTTAAGGCTTTTCTTCCATCTTCTTGCTTCGATCAGAACTCCCTTGAATTGGAAACTCTTGACTCACATGACGTTCCTCGCCATCAAGTTCAGAAGGTTTGCATTCTTCTAATTTTGTGTTCCAGAAAAGTTTTTGTTACATTGTGTTTGTTTCATTACAAATTTGTGGAAACATTTGAATTTGTAGCTAATATTTTCAAAACTTGGGAGTACACAACAAACACGAAGAAACTCATATAAGGAAATTAGTGTTCATAAGCTTGATTTTAAAAACAAAAAAACCAAATAACGACCTCTTAAGTTCCTCTCTTTCTTCTTTTCTGATCATAAATGATGAACTGGTAATCTTGAAGGAAACAGTGGAATTCCAGACCCGATCTAGATTTGATTGCTTTGAAATTTTAAGTGATACGATCTGCAGTAGATGATATACTATAGCTCCTTAATCAATCTGAATTGGGGAAACTTGAAAAGGCGACTTTTATTTCGGTGGGTGGAGGTTTTTCTTTTTTTAGCATTATTGGACTTTACGAAAAGCTTGAACAAGACTTAACGAAGATGGTATTTCCATAAAACCACGGCTTAACTCTGAAGCAAACCTGATGCTTTTATTCAATCAAAAAAGCACAACTATAAAGTTTCACCAGTTGTGCATGGTGCCTTTATGAGTCTTTGTGCCTTTTGTATAAACTCAAAAAGTAATCCATTTTAACATTGTTTAAGCTAGTTTGGATTACTTGACGAAAGTGTTGAATTCACATGTTCTCTTTTATTGCACCTTGCAGGTTATCTGTTCCTTGTGTGATACAGAACAAGATGTGAGTTCCTTGAGAGCGACACTGTAATTAAAACATATTGAGAAACATAACTTCAAATGGGTGTAATTACCTTGCTGAATTCCTTTTTGTTTCAAACAGGTTCAGCAGTACTGCATGAACTGTGGAGTCTGTTTTGGGAAGTACTTCTGCAGCATATGCAAATTCTTCGATGATGACGTGAGTTAACTCGATGGAGAAACGTTTTCATAGTCAGTTTCTGATGCTGTGTTTGATTTCTAATCGCTTCAGTTTGTTCCCTTTTCAGGTTTCAAAGAACCAATACCACTGTGATGAATGTGGAATCTGCAGGTATAAGTTTTTGAAGCTTTTCTTTAATCTTTTGGTTACGCTTTTCAATATGTTATCTTAATTCTATTTTGCAGGACTGGGGGGAAGGAAAATTTCTTCCACTGTAAGACATGTGGTGAGTGAGAGACAAAACTCAAACTCAAACTCAAACTCAAGCCTGTAATAGCTAGAAAACAGTTACAAAACTTACTTTTGTGTTCTTGCAATGTTTGTCGTAGGATGTTGTTATTCGAAATTAATGAAAGACGGGCATCGTTGCATCGAAAGAGCAATGCATCATAGCTGCCCTGTCTGCTTTGAGGTAAGGAAAACCAATGCACAACTATCTTATGTTAATGCCTGCCTATTGGTTGAATGAACATTACTCGTTTTGATATGTCCGAATTCTTCAATGCAGTTTCTATTTGACACAACAAAAGACATCAGTGTCTTACCTTGTGGCCACACCATGCATTTGAAATGTGCTAAAGAGATGGAGTCACATTTCCAGTAAGTACATCACATTTGAAATGCTTATATTAGTCTTTTCACTCGATCTTGTTTGAAATGAAGCAGGTGCGAGGTCAATAAGTTATTTTCATTTATGATTGTGTAGAATATGTTCCTGATTTATATAAGTTGCTACAATGTGGTTCTTTTATCGTTCGTCCAGGTACTCTTGCCCTGTATGCTCAAAGTCAATCTGTGACATGTCTAGGTTGTGGGCAAAGCTTGATACAGTGGTAATCAGATCATATTACTCTTCCTTTTCTTAATCTCCCCGAACAATAATGTGAACTGAATTCATTCATCAAGTTCTTGATTTCTTTTTCTCAAACTATGAATGCAGATTGCTTCAACACCGATGCCTGATATGTACAAAAACAAGAAGGTAAATACCCTTTCAACAATGTTTTCGGCTCTCTGGTCTTAATTTCCTGCTTGTTCTTAAGAGATTTGAAGAATAAAACGGATGCCCCTTTCTTGGATCTTTATGCAAACGGAGAGAAGGCAGTTATAAATCTTAGAGTATTTGAATAGTTGAATTTACTATAACCCATCAACTTAAGTTATCATATCGTTATGCATACACCTACGCAGGTGTGGATTCTATGTAATGACTGTGGTGCAGAAGGTGAAGTTCAATTCCATGTGATAGCTCATAAATGCTTGGACTGCAAGTCCTACAACACCAAACAGACAATAGGAAGAGGACCTCCTGCTTCTTGCTCATCGACAGCTGTGACCGAGATAGTGAGATAAACTTTCCTGCCAAAAGAAGTTTTAAGAGGCGAAATTCTTGGTCTTGCTCGCCACATTATGGTTATCTTATCATAATCATAATAAGACTATTGCACCAAGTAAGCTTGGGTCAAATGGTTCTCAAGACTAGAATGGGATCGCGCTACCAAATGTCACCGTTCCTCATGGCATGTGTATATAAGTAAATTTGGATGGATTATGTGGTTTTAAGGTTCTATTTGGCTGCTCGTTCCAATATTTGGTGGTGCTGTTCTCTTTTAGTTTTTAGCAGTTTACGTCCGAGTGTCCTAAACAAAACCATTGAGTAGTCACAATCCAAAATGTGCATCACAAAGTAGTGATTCTCGATCACATCTTTGAAAAATCATAAAAAGTTCACAGCAAAAACTTTGAAGACATGAAATAGTGACAAGATCATTGTGATTTGCTTTTTTAGCGTCTCGAGTAAGTTCAACGGCCCAAACCTACTCCTAGCAGATATTGTCCATTTTGGCCCGTTACATATCATCACCGTCACCC

mRNA sequence

CTCGCACTGTGCAGATCTCTGTAACGAAAGGATTTTTTTTTCTTTTTCTGAGTGCTGTTTGTTCTTGCTCTTTTTCCGTTTCAATGGAGATTCATATTCAGGCTTCGTTATCTTCGCGTTCATCATCTGAATCTGAAATTTCTGCTTCTGATAATATAGATGCTGCGGATCGTGAATCTGGGAATTTCGGGTGCTCGCATTATCGAAGGAGATGCAAAATTAGAACGCCCTGTTGCGATGAGATCTTTGATTGTAGGCATTGTCACAATGAAGCGAAGAACTCCCTTGAATTGGAAACTCTTGACTCACATGACGTTCCTCGCCATCAAGTTCAGAAGGTTATCTGTTCCTTGTGTGATACAGAACAAGATGTTCAGCAGTACTGCATGAACTGTGGAGTCTGTTTTGGGAAGTACTTCTGCAGCATATGCAAATTCTTCGATGATGACGTTTCAAAGAACCAATACCACTGTGATGAATGTGGAATCTGCAGGACTGGGGGGAAGGAAAATTTCTTCCACTGTAAGACATGTGGATGTTGTTATTCGAAATTAATGAAAGACGGGCATCGTTGCATCGAAAGAGCAATGCATCATAGCTGCCCTGTCTGCTTTGAGTTTCTATTTGACACAACAAAAGACATCAGTGTCTTACCTTGTGGCCACACCATGCATTTGAAATGTGCTAAAGAGATGGAGTCACATTTCCAGTACTCTTGCCCTGTATGCTCAAAGTCAATCTGTGACATGTCTAGGTTGTGGGCAAAGCTTGATACAGTGATTGCTTCAACACCGATGCCTGATATGTACAAAAACAAGAAGGTGTGGATTCTATGTAATGACTGTGGTGCAGAAGGTGAAGTTCAATTCCATGTGATAGCTCATAAATGCTTGGACTGCAAGTCCTACAACACCAAACAGACAATAGGAAGAGGACCTCCTGCTTCTTGCTCATCGACAGCTGTGACCGAGATAGTGAGATAAACTTTCCTGCCAAAAGAAGTTTTAAGAGGCGAAATTCTTGGTCTTGCTCGCCACATTATGGTTATCTTATCATAATCATAATAAGACTATTGCACCAAGTAAGCTTGGGTCAAATGGTTCTCAAGACTAGAATGGGATCGCGCTACCAAATGTCACCGTTCCTCATGGCATGTGTATATAAGTAAATTTGGATGGATTATGTGGTTTTAAGGTTCTATTTGGCTGCTCGTTCCAATATTTGGTGGTGCTGTTCTCTTTTAGTTTTTAGCAGTTTACGTCCGAGTGTCCTAAACAAAACCATTGAGTAGTCACAATCCAAAATGTGCATCACAAAGTAGTGATTCTCGATCACATCTTTGAAAAATCATAAAAAGTTCACAGCAAAAACTTTGAAGACATGAAATAGTGACAAGATCATTGTGATTTGCTTTTTTAGCGTCTCGAGTAAGTTCAACGGCCCAAACCTACTCCTAGCAGATATTGTCCATTTTGGCCCGTTACATATCATCACCGTCACCC

Coding sequence (CDS)

ATGGAGATTCATATTCAGGCTTCGTTATCTTCGCGTTCATCATCTGAATCTGAAATTTCTGCTTCTGATAATATAGATGCTGCGGATCGTGAATCTGGGAATTTCGGGTGCTCGCATTATCGAAGGAGATGCAAAATTAGAACGCCCTGTTGCGATGAGATCTTTGATTGTAGGCATTGTCACAATGAAGCGAAGAACTCCCTTGAATTGGAAACTCTTGACTCACATGACGTTCCTCGCCATCAAGTTCAGAAGGTTATCTGTTCCTTGTGTGATACAGAACAAGATGTTCAGCAGTACTGCATGAACTGTGGAGTCTGTTTTGGGAAGTACTTCTGCAGCATATGCAAATTCTTCGATGATGACGTTTCAAAGAACCAATACCACTGTGATGAATGTGGAATCTGCAGGACTGGGGGGAAGGAAAATTTCTTCCACTGTAAGACATGTGGATGTTGTTATTCGAAATTAATGAAAGACGGGCATCGTTGCATCGAAAGAGCAATGCATCATAGCTGCCCTGTCTGCTTTGAGTTTCTATTTGACACAACAAAAGACATCAGTGTCTTACCTTGTGGCCACACCATGCATTTGAAATGTGCTAAAGAGATGGAGTCACATTTCCAGTACTCTTGCCCTGTATGCTCAAAGTCAATCTGTGACATGTCTAGGTTGTGGGCAAAGCTTGATACAGTGATTGCTTCAACACCGATGCCTGATATGTACAAAAACAAGAAGGTGTGGATTCTATGTAATGACTGTGGTGCAGAAGGTGAAGTTCAATTCCATGTGATAGCTCATAAATGCTTGGACTGCAAGTCCTACAACACCAAACAGACAATAGGAAGAGGACCTCCTGCTTCTTGCTCATCGACAGCTGTGACCGAGATAGTGAGATAA

Protein sequence

MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHCHNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR
Homology
BLAST of CmoCh20G005560 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 4.2e-124
Identity = 189/260 (72.69%), Postives = 219/260 (84.23%), Query Frame = 0

Query: 32  SGNFGCSHYRRRCKIRTPCCDEIFDCRHCHNEAKNSLELETLDSHDVPRHQVQKVICSLC 91
           SG++GCSHYRRRCKIR PCCDEIFDCRHCHNEAK+SL +E    H++PRH+V KVICSLC
Sbjct: 22  SGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVICSLC 81

Query: 92  DTEQDVQQYCMNCGVCFGKYFCSICKFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCG 151
           +TEQDVQQ C NCGVC GKYFCS CKFFDDD+SK QYHCDECGICRTGG+ENFFHCK C 
Sbjct: 82  ETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHCKRCR 141

Query: 152 CCYSKLMKDGHRCIERAMHHSCPVCFEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYS 211
           CCYSK+M+D H+C+E AMHH+CPVCFE+LFD+T+DI+VL CGHTMHL+C K+M  H +Y+
Sbjct: 142 CCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMGLHNRYT 201

Query: 212 CPVCSKSICDMSRLWAKLDTVIASTPMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLD 271
           CPVCSKSICDMS LW KLD  +A+ PMP MY+NK VWILCNDCG+   V+FH+IAHKC  
Sbjct: 202 CPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGSNTNVRFHLIAHKCSS 261

Query: 272 CKSYNTKQTIGRGPPASCSS 292
           C SYNT+QT       SCSS
Sbjct: 262 CGSYNTRQTQRGSDSHSCSS 281

BLAST of CmoCh20G005560 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.2e-110
Identity = 172/284 (60.56%), Postives = 220/284 (77.46%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSSESEISASDNIDAADRES-----GNFGCSHYRRRCKIRTPCCDEIF 60
           +E H   SL S     +E   ++++D  D E        +GC+HYRRRC+IR PCC+EIF
Sbjct: 22  VEEHALVSLLSDEKYATE--KTEDVDPDDYEKLEEGIMQYGCAHYRRRCRIRAPCCNEIF 81

Query: 61  DCRHCHNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSI 120
           DCRHCHNE KNS++++ +  H++PRH+VQ+VICSLC TEQ+V+Q C++CGVC GKYFC +
Sbjct: 82  DCRHCHNETKNSIKIDAVKRHELPRHEVQQVICSLCGTEQEVRQVCISCGVCMGKYFCEV 141

Query: 121 CKFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPV 180
           CK FDDDVSK QYHC+ CGICR GGKENFFHC  CGCCYS ++K+ H C+E AMHH CP+
Sbjct: 142 CKLFDDDVSKQQYHCNGCGICRIGGKENFFHCSKCGCCYSIVLKNSHACVEGAMHHDCPI 201

Query: 181 CFEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIAS 240
           CFE+LF++T D+SVLPCGHT+H+KC +EME H Q++CP+CSKS+CDMS+ W +LD  +A+
Sbjct: 202 CFEYLFESTNDVSVLPCGHTIHVKCLREMEEHCQFACPLCSKSVCDMSKAWERLDEELAT 261

Query: 241 TPMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQ 280
             + D   NK V ILCNDCGA  EVQFH+IAHKC  CKSYNT+Q
Sbjct: 262 --ISDTCDNKMVRILCNDCGATSEVQFHLIAHKCQKCKSYNTRQ 301

BLAST of CmoCh20G005560 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 6.4e-96
Identity = 141/244 (57.79%), Postives = 184/244 (75.41%), Query Frame = 0

Query: 35  FGCSHYRRRCKIRTPCCDEIFDCRHCHNEAKNSLELETLDSHDVPRHQVQKVICSLCDTE 94
           FGC HY+RRC+IR PCC+E+FDCRHCHNE+ ++L     D HD+ R  V++VICS+CDTE
Sbjct: 16  FGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLR-NIYDRHDLVRQDVKQVICSVCDTE 75

Query: 95  QDVQQYCMNCGVCFGKYFCSICKFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCGCCY 154
           Q   Q C NCGV  G+YFCSIC F+DDD  K Q+HCD+CGICR GG+ENFFHCK CG CY
Sbjct: 76  QPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHCKKCGSCY 135

Query: 155 SKLMKDGHRCIERAMHHSCPVCFEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPV 214
           +  +++ HRC+E +M H CP+C+E+LFD+ KD +V+ CGHTMH++C  EM    ++ CP+
Sbjct: 136 AVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKRDKFCCPI 195

Query: 215 CSKSICDMSRLWAKLDTVIASTPMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKS 274
           CS+S+ DMS+ W +LD  I +T MP  Y++KKVWILCNDC    EV FH+I  KC  C+S
Sbjct: 196 CSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQKCGHCRS 255

Query: 275 YNTK 279
           YNT+
Sbjct: 256 YNTR 258

BLAST of CmoCh20G005560 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.5e-84
Identity = 132/243 (54.32%), Postives = 169/243 (69.55%), Query Frame = 0

Query: 35  FGCSHYRRRCKIRTPCCDEIFDCRHCHNEAKNSLELETLDSHDVPRHQVQKVICSLCDTE 94
           FGC HYRRRC+IR PCC+++F CRHCHNE+       T D H++ RH V+ VIC +CDTE
Sbjct: 16  FGCKHYRRRCRIRAPCCNDVFHCRHCHNES-------TKDGHELDRHAVESVICLVCDTE 75

Query: 95  QDVQQYCMNCGVCFGKYFCSICKFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCGCCY 154
           Q V Q C NCGVC G+YFCS CKFFDDDV +  +HC +CGICR GGK+NFFHC+ CG CY
Sbjct: 76  QPVAQVCYNCGVCMGEYFCSACKFFDDDVDREHFHCQDCGICRVGGKDNFFHCEKCGSCY 135

Query: 155 SKLMKDGHRCIERAMHHSCPVCFEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPV 214
           S  ++D H CIE +M ++CP+C+E+LFD+ ++ SVL CGHTMHL+C  EM  H ++SCP+
Sbjct: 136 SVSLRDKHCCIENSMKNNCPICYEYLFDSLRETSVLRCGHTMHLQCFHEMLKHDKFSCPI 195

Query: 215 CSKSICDMSRLWAKLDTVIASTPMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKS 274
           CS  I DM +    LD  I +  +   Y  K  WI+CNDC    +V   V  HKC  C+S
Sbjct: 196 CSMPIFDMDKFLRALDAEIEANMLHIDYMGKG-WIVCNDCRDTTQVYARVAGHKCCHCQS 250

Query: 275 YNT 278
           +NT
Sbjct: 256 HNT 250

BLAST of CmoCh20G005560 vs. ExPASy Swiss-Prot
Match: Q9CR50 (RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=10090 GN=Rchy1 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.1e-66
Identity = 115/254 (45.28%), Postives = 149/254 (58.66%), Query Frame = 0

Query: 33  GNFGCSHYRRRCKIRTPCCDEIFDCRHCHNEAKNSLELETLDSHDVPRHQVQKVICSLCD 92
           G  GC HY R C ++ PCCD+++ CR CH         +T + H + R +V++V C  C+
Sbjct: 16  GPRGCEHYDRACLLKAPCCDKLYTCRLCH---------DTNEDHQLDRFKVKEVQCINCE 75

Query: 93  TEQDVQQYCMNCGVCFGKYFCSICKFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCGC 152
             Q  QQ C +C   FG+Y+CSIC  FD D  K QYHC+ CGICR G KE+FFHC  C  
Sbjct: 76  KLQHAQQTCEDCSTLFGEYYCSICHLFDKD--KRQYHCESCGICRIGPKEDFFHCLKCNL 135

Query: 153 CYSKLMKDGHRCIERAMHHSCPVCFEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYSC 212
           C +  ++  H+CIE     +CP+C E +  +     VLPCGH +H  C +EM     Y C
Sbjct: 136 CLTTNLRGKHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKE-GYRC 195

Query: 213 PVCSKSICDMSRLWAKLDTVIASTPMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLDC 272
           P+C  S  DM+R W +LDT +A TPMP  Y+N  V ILCNDC     VQFH++  KC  C
Sbjct: 196 PLCMHSALDMTRYWRQLDTEVAQTPMPSEYQNVTVDILCNDCNGRSTVQFHILGMKCKLC 255

Query: 273 KSYNTKQTIGRGPP 287
            SYNT Q  GR  P
Sbjct: 256 DSYNTAQAGGRRVP 257

BLAST of CmoCh20G005560 vs. ExPASy TrEMBL
Match: A0A6J1FVA6 (E3 ubiquitin-protein ligase RZFP34-like OS=Cucurbita moschata OX=3662 GN=LOC111448428 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 3.2e-183
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60
           MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC
Sbjct: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60

Query: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120
           HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD
Sbjct: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120

Query: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180
           DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL
Sbjct: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180

Query: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240
           FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD
Sbjct: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240

Query: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 300
           MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR
Sbjct: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 299

BLAST of CmoCh20G005560 vs. ExPASy TrEMBL
Match: A0A6J1JG98 (E3 ubiquitin-protein ligase RZFP34-like OS=Cucurbita maxima OX=3661 GN=LOC111484118 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 5.1e-181
Identity = 295/299 (98.66%), Postives = 295/299 (98.66%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60
           MEIH QASLSSRSSSESEISASDN DAADRESGNFGCSHYRRRCKIR PCCDEIFDCRHC
Sbjct: 1   MEIHFQASLSSRSSSESEISASDNTDAADRESGNFGCSHYRRRCKIRAPCCDEIFDCRHC 60

Query: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120
           HNEAKNSLELETLD HDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD
Sbjct: 61  HNEAKNSLELETLDPHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120

Query: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180
           DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL
Sbjct: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180

Query: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240
           FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD
Sbjct: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240

Query: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 300
           MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR
Sbjct: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 299

BLAST of CmoCh20G005560 vs. ExPASy TrEMBL
Match: A0A0A0KGJ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G148230 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.3e-163
Identity = 269/298 (90.27%), Postives = 276/298 (92.62%), Query Frame = 0

Query: 4   HIQASLSSRSSS---ESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 63
           HIQASLSS +SS   E  +S S N  AADRESGNFGC HYRRRCKIR PCCDEIFDCRHC
Sbjct: 5   HIQASLSSSASSSSIEPPLSESHNTCAADRESGNFGCLHYRRRCKIRAPCCDEIFDCRHC 64

Query: 64  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 123
           HNEAKNSLELETLD HDVPRHQ+QKVICSLCDTEQDVQQYC NCGVCFGKYFCSICKFFD
Sbjct: 65  HNEAKNSLELETLDPHDVPRHQIQKVICSLCDTEQDVQQYCSNCGVCFGKYFCSICKFFD 124

Query: 124 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 183
           DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL
Sbjct: 125 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 184

Query: 184 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 243
           FDTTKDISVLPCGHT+HL+CAKEMESHFQYSCPVCSKSICDMSRLW KLD VIAST MPD
Sbjct: 185 FDTTKDISVLPCGHTIHLECAKEMESHFQYSCPVCSKSICDMSRLWEKLDKVIASTLMPD 244

Query: 244 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIV 299
           +YKNKKVWILCNDCGAEGEVQFHV+AHKCLDC SYNTKQTIGR P ASCSST V EIV
Sbjct: 245 IYKNKKVWILCNDCGAEGEVQFHVVAHKCLDCNSYNTKQTIGRRPTASCSSTTVPEIV 302

BLAST of CmoCh20G005560 vs. ExPASy TrEMBL
Match: A0A1S3CNU2 (E3 ubiquitin-protein ligase MIEL1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503036 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 4.8e-163
Identity = 268/309 (86.73%), Postives = 278/309 (89.97%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSS-----------ESEISASDNIDAADRESGNFGCSHYRRRCKIRTP 60
           M+IHIQASLSS SSS           ES +S   N  A DRESGNFGC HYRRRCKIR P
Sbjct: 1   MDIHIQASLSSSSSSSSSSSSSSSSIESTVSEPHNTYAEDRESGNFGCLHYRRRCKIRAP 60

Query: 61  CCDEIFDCRHCHNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFG 120
           CCDE+FDCRHCHNEAKNSLELETLD HDVPRHQ+QKVICSLCDTEQDVQQYC NCGVCFG
Sbjct: 61  CCDEVFDCRHCHNEAKNSLELETLDPHDVPRHQIQKVICSLCDTEQDVQQYCSNCGVCFG 120

Query: 121 KYFCSICKFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAM 180
           KYFCSIC+FFDDDVSK+QYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAM
Sbjct: 121 KYFCSICRFFDDDVSKSQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAM 180

Query: 181 HHSCPVCFEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKL 240
           HHSCPVCFEFLFDTTKDISVLPCGHT+HL+CAKEMESHFQYSCPVCSKSICDMSRLW KL
Sbjct: 181 HHSCPVCFEFLFDTTKDISVLPCGHTIHLECAKEMESHFQYSCPVCSKSICDMSRLWVKL 240

Query: 241 DTVIASTPMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASC 299
           D VIASTPMPD+YKNKKVWILCNDCGAEGEVQFHV+AHKCLDC SYNTKQTIGR   ASC
Sbjct: 241 DKVIASTPMPDIYKNKKVWILCNDCGAEGEVQFHVVAHKCLDCNSYNTKQTIGRRRTASC 300

BLAST of CmoCh20G005560 vs. ExPASy TrEMBL
Match: A0A6J1CF16 (E3 ubiquitin-protein ligase RZFP34 OS=Momordica charantia OX=3673 GN=LOC111011097 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.8e-157
Identity = 257/299 (85.95%), Postives = 269/299 (89.97%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60
           M   I+AS S  SSSES +SAS N DA +R+SGNFGCSHYRRRCKIR PCCDEIFDCRHC
Sbjct: 1   MATDIEAS-SFSSSSESRVSASQNTDAIERDSGNFGCSHYRRRCKIRAPCCDEIFDCRHC 60

Query: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120
           HNEAKNSLE +TLD HD+PRHQVQKVICSLCDTEQDVQQYC+NCGVC GKYFCSICKFFD
Sbjct: 61  HNEAKNSLETKTLDPHDIPRHQVQKVICSLCDTEQDVQQYCINCGVCLGKYFCSICKFFD 120

Query: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180
           DDVSKNQYHCDECGICR GGK+NFFHCKTC CCYSK+MKDGHRCIERAMHHSCPVCFEFL
Sbjct: 121 DDVSKNQYHCDECGICRIGGKDNFFHCKTCECCYSKVMKDGHRCIERAMHHSCPVCFEFL 180

Query: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240
           FDTTKDISVLPCGHT+HL+CAKEMESHFQYSCPVCSKSICDMSRLW KLD VIASTPMPD
Sbjct: 181 FDTTKDISVLPCGHTIHLECAKEMESHFQYSCPVCSKSICDMSRLWEKLDKVIASTPMPD 240

Query: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 300
            YKNKKVWILCNDCG E EVQFH+IAHKCL C SYNTKQT GRG  A C ST V EIVR
Sbjct: 241 TYKNKKVWILCNDCGTEDEVQFHIIAHKCLKCNSYNTKQTRGRGSTAPCPSTVVPEIVR 298

BLAST of CmoCh20G005560 vs. NCBI nr
Match: XP_022943779.1 (E3 ubiquitin-protein ligase RZFP34-like [Cucurbita moschata])

HSP 1 Score: 650.6 bits (1677), Expect = 6.6e-183
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60
           MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC
Sbjct: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60

Query: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120
           HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD
Sbjct: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120

Query: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180
           DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL
Sbjct: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180

Query: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240
           FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD
Sbjct: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240

Query: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 300
           MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR
Sbjct: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 299

BLAST of CmoCh20G005560 vs. NCBI nr
Match: KAG6570821.1 (E3 ubiquitin-protein ligase RZFP34, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 644.4 bits (1661), Expect = 4.7e-181
Identity = 296/299 (99.00%), Postives = 296/299 (99.00%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60
           MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIR PCCDEIFDCRHC
Sbjct: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRAPCCDEIFDCRHC 60

Query: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120
           HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFC ICKFFD
Sbjct: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCIICKFFD 120

Query: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180
           DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL
Sbjct: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180

Query: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240
           FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD
Sbjct: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240

Query: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 300
           MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSST VTEIVR
Sbjct: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTPVTEIVR 299

BLAST of CmoCh20G005560 vs. NCBI nr
Match: XP_022986353.1 (E3 ubiquitin-protein ligase RZFP34-like [Cucurbita maxima])

HSP 1 Score: 643.3 bits (1658), Expect = 1.1e-180
Identity = 295/299 (98.66%), Postives = 295/299 (98.66%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60
           MEIH QASLSSRSSSESEISASDN DAADRESGNFGCSHYRRRCKIR PCCDEIFDCRHC
Sbjct: 1   MEIHFQASLSSRSSSESEISASDNTDAADRESGNFGCSHYRRRCKIRAPCCDEIFDCRHC 60

Query: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120
           HNEAKNSLELETLD HDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD
Sbjct: 61  HNEAKNSLELETLDPHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120

Query: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180
           DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL
Sbjct: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180

Query: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240
           FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD
Sbjct: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240

Query: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 300
           MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR
Sbjct: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 299

BLAST of CmoCh20G005560 vs. NCBI nr
Match: XP_023513373.1 (E3 ubiquitin-protein ligase RZFP34 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 640.2 bits (1650), Expect = 8.9e-180
Identity = 294/299 (98.33%), Postives = 294/299 (98.33%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60
           MEIHIQASLSSRS SESEISASDN DAADRESGNFGCSHYRRRCKIR PCCDEIFDCRHC
Sbjct: 1   MEIHIQASLSSRSLSESEISASDNTDAADRESGNFGCSHYRRRCKIRAPCCDEIFDCRHC 60

Query: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120
           HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD
Sbjct: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120

Query: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180
           DDVSKNQYHCDECGICRTGGKENFFHCK CGCCYS LMKDGHRCIERAMHHSCPVCFEFL
Sbjct: 121 DDVSKNQYHCDECGICRTGGKENFFHCKRCGCCYSNLMKDGHRCIERAMHHSCPVCFEFL 180

Query: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240
           FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD
Sbjct: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240

Query: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 300
           MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR
Sbjct: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 299

BLAST of CmoCh20G005560 vs. NCBI nr
Match: KAG7010663.1 (E3 ubiquitin-protein ligase RZFP34 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 640.2 bits (1650), Expect = 8.9e-180
Identity = 294/299 (98.33%), Postives = 294/299 (98.33%), Query Frame = 0

Query: 1   MEIHIQASLSSRSSSESEISASDNIDAADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHC 60
           MEIHIQASLSSRS SESEISASDN DAADRESGNFGCSHYRRRCKIR PCCDEIFDCRHC
Sbjct: 1   MEIHIQASLSSRSLSESEISASDNTDAADRESGNFGCSHYRRRCKIRAPCCDEIFDCRHC 60

Query: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 120
           HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFC ICKFFD
Sbjct: 61  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCIICKFFD 120

Query: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180
           DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL
Sbjct: 121 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 180

Query: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240
           FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD
Sbjct: 181 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 240

Query: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTAVTEIVR 300
           MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSST VTEIVR
Sbjct: 241 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIGRGPPASCSSTPVTEIVR 299

BLAST of CmoCh20G005560 vs. TAIR 10
Match: AT5G22920.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 445.7 bits (1145), Expect = 3.0e-125
Identity = 189/260 (72.69%), Postives = 219/260 (84.23%), Query Frame = 0

Query: 32  SGNFGCSHYRRRCKIRTPCCDEIFDCRHCHNEAKNSLELETLDSHDVPRHQVQKVICSLC 91
           SG++GCSHYRRRCKIR PCCDEIFDCRHCHNEAK+SL +E    H++PRH+V KVICSLC
Sbjct: 22  SGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVICSLC 81

Query: 92  DTEQDVQQYCMNCGVCFGKYFCSICKFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCG 151
           +TEQDVQQ C NCGVC GKYFCS CKFFDDD+SK QYHCDECGICRTGG+ENFFHCK C 
Sbjct: 82  ETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHCKRCR 141

Query: 152 CCYSKLMKDGHRCIERAMHHSCPVCFEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYS 211
           CCYSK+M+D H+C+E AMHH+CPVCFE+LFD+T+DI+VL CGHTMHL+C K+M  H +Y+
Sbjct: 142 CCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMGLHNRYT 201

Query: 212 CPVCSKSICDMSRLWAKLDTVIASTPMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLD 271
           CPVCSKSICDMS LW KLD  +A+ PMP MY+NK VWILCNDCG+   V+FH+IAHKC  
Sbjct: 202 CPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGSNTNVRFHLIAHKCSS 261

Query: 272 CKSYNTKQTIGRGPPASCSS 292
           C SYNT+QT       SCSS
Sbjct: 262 CGSYNTRQTQRGSDSHSCSS 281

BLAST of CmoCh20G005560 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 404.4 bits (1038), Expect = 7.7e-113
Identity = 169/276 (61.23%), Postives = 212/276 (76.81%), Query Frame = 0

Query: 14  SSESEISASDNIDA-------ADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHCHNEAKN 73
           + ESE S  + + A        DR    +GC HYRRRC IR PCC+EIF C HCH EAKN
Sbjct: 33  NEESESSTLERVAAESLTNKVLDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKN 92

Query: 74  SLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFDDDVSKN 133
           ++ ++    HD+PRHQV++VIC LC TEQ+V Q C++CGVC GKYFC +CK +DDD SK 
Sbjct: 93  NINVDQKQRHDIPRHQVEQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKK 152

Query: 134 QYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFLFDTTKD 193
           QYHCD CGICR GG+ENFFHC  CGCCYS L+K+GH C+E AMHH CP+CFEFLF++  D
Sbjct: 153 QYHCDGCGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRND 212

Query: 194 ISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPDMYKNKK 253
           ++VLPCGHT+H KC +EM  H+QY+CP+CSKS+CDMS++W K D  IA+TPMP+ Y+N+ 
Sbjct: 213 VTVLPCGHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRM 272

Query: 254 VWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIG 283
           V ILCNDCG + EVQ+HV+A KC +CKSYNT+QT G
Sbjct: 273 VQILCNDCGKKAEVQYHVVAQKCPNCKSYNTRQTRG 308

BLAST of CmoCh20G005560 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 396.4 bits (1017), Expect = 2.1e-110
Identity = 169/286 (59.09%), Postives = 212/286 (74.13%), Query Frame = 0

Query: 14  SSESEISASDNIDA-------ADRESGNFGCSHYRRRCKIRTPCCDEIFDCRHCHNEAKN 73
           + ESE S  + + A        DR    +GC HYRRRC IR PCC+EIF C HCH EAKN
Sbjct: 33  NEESESSTLERVAAESLTNKVLDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKN 92

Query: 74  SLELETLDSHDVPRHQVQ----------KVICSLCDTEQDVQQYCMNCGVCFGKYFCSIC 133
           ++ ++    HD+PRHQV+          +VIC LC TEQ+V Q C++CGVC GKYFC +C
Sbjct: 93  NINVDQKQRHDIPRHQVEQLTRPLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVC 152

Query: 134 KFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVC 193
           K +DDD SK QYHCD CGICR GG+ENFFHC  CGCCYS L+K+GH C+E AMHH CP+C
Sbjct: 153 KLYDDDTSKKQYHCDGCGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPIC 212

Query: 194 FEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIAST 253
           FEFLF++  D++VLPCGHT+H KC +EM  H+QY+CP+CSKS+CDMS++W K D  IA+T
Sbjct: 213 FEFLFESRNDVTVLPCGHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAAT 272

Query: 254 PMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIG 283
           PMP+ Y+N+ V ILCNDCG + EVQ+HV+A KC +CKSYNT+QT G
Sbjct: 273 PMPEPYQNRMVQILCNDCGKKAEVQYHVVAQKCPNCKSYNTRQTRG 318

BLAST of CmoCh20G005560 vs. TAIR 10
Match: AT5G25560.4 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 390.2 bits (1001), Expect = 1.5e-108
Identity = 167/282 (59.22%), Postives = 211/282 (74.82%), Query Frame = 0

Query: 6   QASLSSRSSSESEISASDNIDAADRES-----GNFGCSHYRRRCKIRTPCCDEIFDCRHC 65
           + S  S   S +E S S  ++    ES      + G   Y RRC IR PCC+EIF C HC
Sbjct: 22  EMSRHSHPHSINEESESSTLERVAAESLTNKVLDRGLMEYGRRCCIRAPCCNEIFGCHHC 81

Query: 66  HNEAKNSLELETLDSHDVPRHQVQKVICSLCDTEQDVQQYCMNCGVCFGKYFCSICKFFD 125
           H EAKN++ ++    HD+PRHQV++VIC LC TEQ+V Q C++CGVC GKYFC +CK +D
Sbjct: 82  HYEAKNNINVDQKQRHDIPRHQVEQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYD 141

Query: 126 DDVSKNQYHCDECGICRTGGKENFFHCKTCGCCYSKLMKDGHRCIERAMHHSCPVCFEFL 185
           DD SK QYHCD CGICR GG+ENFFHC  CGCCYS L+K+GH C+E AMHH CP+CFEFL
Sbjct: 142 DDTSKKQYHCDGCGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFL 201

Query: 186 FDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPVCSKSICDMSRLWAKLDTVIASTPMPD 245
           F++  D++VLPCGHT+H KC +EM  H+QY+CP+CSKS+CDMS++W K D  IA+TPMP+
Sbjct: 202 FESRNDVTVLPCGHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPE 261

Query: 246 MYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKSYNTKQTIG 283
            Y+N+ V ILCNDCG + EVQ+HV+A KC +CKSYNT+QT G
Sbjct: 262 PYQNRMVQILCNDCGKKAEVQYHVVAQKCPNCKSYNTRQTRG 303

BLAST of CmoCh20G005560 vs. TAIR 10
Match: AT5G18650.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 352.1 bits (902), Expect = 4.5e-97
Identity = 141/244 (57.79%), Postives = 184/244 (75.41%), Query Frame = 0

Query: 35  FGCSHYRRRCKIRTPCCDEIFDCRHCHNEAKNSLELETLDSHDVPRHQVQKVICSLCDTE 94
           FGC HY+RRC+IR PCC+E+FDCRHCHNE+ ++L     D HD+ R  V++VICS+CDTE
Sbjct: 16  FGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLR-NIYDRHDLVRQDVKQVICSVCDTE 75

Query: 95  QDVQQYCMNCGVCFGKYFCSICKFFDDDVSKNQYHCDECGICRTGGKENFFHCKTCGCCY 154
           Q   Q C NCGV  G+YFCSIC F+DDD  K Q+HCD+CGICR GG+ENFFHCK CG CY
Sbjct: 76  QPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHCKKCGSCY 135

Query: 155 SKLMKDGHRCIERAMHHSCPVCFEFLFDTTKDISVLPCGHTMHLKCAKEMESHFQYSCPV 214
           +  +++ HRC+E +M H CP+C+E+LFD+ KD +V+ CGHTMH++C  EM    ++ CP+
Sbjct: 136 AVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKRDKFCCPI 195

Query: 215 CSKSICDMSRLWAKLDTVIASTPMPDMYKNKKVWILCNDCGAEGEVQFHVIAHKCLDCKS 274
           CS+S+ DMS+ W +LD  I +T MP  Y++KKVWILCNDC    EV FH+I  KC  C+S
Sbjct: 196 CSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQKCGHCRS 255

Query: 275 YNTK 279
           YNT+
Sbjct: 256 YNTR 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFB64.2e-12472.69E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=... [more]
Q5JL961.2e-11060.56Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3... [more]
Q8VZK06.4e-9657.79E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ... [more]
Q10LI11.5e-8454.32E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR... [more]
Q9CR501.1e-6645.28RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=1... [more]
Match NameE-valueIdentityDescription
A0A6J1FVA63.2e-183100.00E3 ubiquitin-protein ligase RZFP34-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JG985.1e-18198.66E3 ubiquitin-protein ligase RZFP34-like OS=Cucurbita maxima OX=3661 GN=LOC111484... [more]
A0A0A0KGJ61.3e-16390.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G148230 PE=4 SV=1[more]
A0A1S3CNU24.8e-16386.73E3 ubiquitin-protein ligase MIEL1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A6J1CF161.8e-15785.95E3 ubiquitin-protein ligase RZFP34 OS=Momordica charantia OX=3673 GN=LOC11101109... [more]
Match NameE-valueIdentityDescription
XP_022943779.16.6e-183100.00E3 ubiquitin-protein ligase RZFP34-like [Cucurbita moschata][more]
KAG6570821.14.7e-18199.00E3 ubiquitin-protein ligase RZFP34, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022986353.11.1e-18098.66E3 ubiquitin-protein ligase RZFP34-like [Cucurbita maxima][more]
XP_023513373.18.9e-18098.33E3 ubiquitin-protein ligase RZFP34 [Cucurbita pepo subsp. pepo][more]
KAG7010663.18.9e-18098.33E3 ubiquitin-protein ligase RZFP34 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G22920.13.0e-12572.69CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.17.7e-11361.23CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.32.1e-11059.09CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.41.5e-10859.22CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G18650.14.5e-9757.79CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 173..215
e-value: 4.8E-7
score: 39.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 172..215
e-value: 1.8E-6
score: 28.2
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 173..216
score: 11.633604
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 160..235
e-value: 1.1E-9
score: 39.4
IPR008913Zinc finger, CHY-typePFAMPF05495zf-CHYcoord: 37..118
e-value: 4.0E-19
score: 68.9
IPR008913Zinc finger, CHY-typePROSITEPS51266ZF_CHYcoord: 30..106
score: 25.453079
NoneNo IPR availableGENE3D2.20.28.10coord: 244..283
e-value: 1.3E-18
score: 67.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR21319RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1coord: 16..284
NoneNo IPR availablePANTHERPTHR21319:SF54E3 UBIQUITIN-PROTEIN LIGASE RZFP34coord: 16..284
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 171..222
IPR039512RCHY1, zinc-ribbonPFAMPF14599zinc_ribbon_6coord: 220..277
e-value: 3.0E-25
score: 88.0
IPR017921Zinc finger, CTCHY-typePROSITEPS51270ZF_CTCHYcoord: 108..172
score: 34.623577
IPR037275Zinc finger, CTCHY-type superfamilySUPERFAMILY161245Zinc hairpin stackcoord: 109..166
IPR037274Zinc finger, CHY-type superfamilySUPERFAMILY161219CHY zinc finger-likecoord: 32..108

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G005560.1CmoCh20G005560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding