CmoCh20G005030 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G005030
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRING/U-box superfamily protein
LocationCmo_Chr20: 2404322 .. 2407981 (+)
RNA-Seq ExpressionCmoCh20G005030
SyntenyCmoCh20G005030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTAAACCCTCTCCAAATCCGTCGGCTAAGACTTCATAGTTTCCGAGCAGCTTCGTGATTCCATGGACGCCTTAGGATTCGAATCGGACTTCGAATCGGACCTCGACCTTGCCTACAACCTCCAGCTCGAGGAAGCTCTCGCCGCATCACTCGCTTCATCCTCCTCCTCCGCCTCAATTCCTCGGCCGGGATTTCAAGAATTCGAACGCGATGATATCACTAGAACCGCCACACTCCACTCCAGGGACATCGAGAATGACGATCAGATATTCCAAGATTGGTTGCAATCGGAGTTTGAGATGCGCAGGACTGTCGGGGAAGTTGATCGTCGGGTTCGTAATCACGGTTTCGAGAGGGAGATTCTAAACATTTCCGATGATGACTGTGACGAGTGGGGTGATACTTCTCAGAATCACTTCTTCGGCGAGGGATGCTCGAAGGGCGTTGAGAATCAGGGCGTTTTTAAGCTCTATTTCAAGGGTTTGGTGAGTGAGGAAGGGATTGGAATTGAGAGACGTTTGGTAGCTGGAATCGGTGTTGCTATTTGCAACCCGGAGGGCGAATTAGTGTTCGAGGTGAAAAAACACCTTGTAGGGAATGAAAGGAGTAAAATCGTGGCTGAGTTTAAGGCTTTGATCACAGGACTGGACGCAGCTATGGATTTGAAATTGAAGCGCCTTTGTTTTTACTGCGACTACTACCCTCTTTATCAATTTGTAAGCCTTCTCTGTGCTTTTGTTTGTTGTTTTAATCTTGAACAAATTTCTTGCAATTACTTGTTTATCCACAACCTCATGATGTTTTAACTTTGAAGTGCTCCCAAGATCATTCTGTATTATAAAACTTTAGCTGTCTTCAATAATTATCTGGTTTCTTGAGCACCTACTAAGGATGTAGGACTTCAATGGCGAAGATGACTGTAAAACTTGTATTGACAATGTTGGTTAAAACCCATTTTAAATCAATTCTTAGGCTACTTGAGGAACTAGATCTGTTTCTATTTTTTACACGAGTGGATGGAATTGCAAAATGATGCAATGTGAAGGTTCCTTGTTTTAGTTCCAAACTTTAAGGATAGACCCCATAATTTTCAAGCAACTTTGATAAAGAAATGGTGACGATTTTCATGTCGAGCATCAAGTTGATAGAAAGGAATCCTAAACTCATTCTCCACTAGCAGAAATATCATATTGAGCTTGACAAACCTTGAAGAGGATGGATGTGCTTTAAAGTGATATTGAAAATGGTAAGATATACTAACAGCTGATGTTGCAGATGGCAGTTATTTGACTATATTGGGCTAGTATTATTATTGGGGCGTCTTACCATTTTTAGTTATGGGTCCTCAAGGCTGTAGCTTGTAGTCATCCTTTCCAAATAATGGTTTAAGGTCTGCTTTTAACAAAAGCAATTTTCCGAAAAAGGTTATTTGGATGTGTTCCTAAGCTTCTACGTTTTTGTTTGAAATGGTTGTTCTCAGGTTAAGTTTTTGATTTGCAATTTATTTCTAGAACTACAACAAACAATCTTATTATTTAAGCCGTGAACAGGTTAGTGGCAAATGGCCACCGAAGCAGCGCAAGATTGTCGCTCTACTCGATCGGGTAACTCATCTGCAACAAGCACATTTTGACTCTTGCTATCCCAGGCTTGTGGCTAGGCATGATATCAAATTTGCGTTCAAACTAGCGAGAGAGGCAATAGTTCCTCAGATCACACGACCTGTTGATCCTGCCCCAAAGAAGAAGCTACATGAGACTTGTATTATTTGTTTGGAGGAGTGTGATTTTAATAGGATGTTTGCAGTCGATGGCTGCTCGCATCGGTACTGCTCTTCTTGTATGAAACAACATGTGGAAGTGAAATTACTTCAAGGATTGGTGCCTAAGTGTCCGCATGATGGGTGTAAGTTTGACCTTAATGTCGATAGCTGTGCAAAATTCTTGACACCTAAAGATATGGTTACGATGCGCCAACGTATTAAAGAAGCTTCCATACCCGTTTCTGAGAAAGTTTATTGTCCATATCCAAGATGTTCGGCTCTAATGACAAAAGTCGAGGTCTTGGAATACACTAGAGACATCTTTAGTGGTGCCAACCAATCTGGAATGCGGAAATGCATGAAGTGTCATGGTCTTTTCTGTGTCAACTGCAAGGTTCCTTGGCATAATCGTGTTTCCTGCAATGACTACAAAAGGTCAAATAATCTTCCCACTGATGATGTGAAATTGAATTACCTGGCTTCAACATGTCTGTGGCGCCAATGTGTGAAGTGTAATCACATGATTGAACTTGCTGAAGGTTGCTATCACATGACTTGCAGGTAACCTTATTTCTATCGAAACATAACTCTGTTAATAACTCTCTTTAACCTGAATTTACACGTTATTGTGGAATTTAGAATTTTCCATTATAATTACTCTTGGCCTGGTCCAAACTCAGGTGTTTCCATCTGACTTTAAATTCTTGTTTGGCATGTAATTCTGACAGTCTTTACACTTTTCTGCATAACATGTTGAATTTGATTGAAGGGCAGGAGTCAAAATAGTACTATACATGAAAAATGGAATGAAACAAGCTTTATTTGTTTGATTTCGTATACATTAGGTTTATCGATTGTGGAACTGAAACAGCCCAAACCCACTGCTAGCAGATATTGTCTATTTTTAGGCTTTCCTTTCAAGGTTTTAAAACGCGTTTACTAGGGATAGGTTTCCACACCCTTATAAGGAATGCTTCATTCCCCTCTCTAACCGATTTGGGATCTCACCATCCACTCCCCTTGGGAGCCTAACATCCTCATTGGCACACCGCCCGGTGTCTGGCTTTGATACCATTTGTAACAGTCCAAATTCACCGCTAGCATATATTGTTCTCTTTGGTCTCTCCCTTTTGGGCTTCCCCTCAAAGTTTTTAAAACGTGTCTGTTAGGGAAAGGTTTCCAAACACTCGTAAGAAATGCCTCATTCCCCTCTCCAACCAATGTGGGATTTCACAATCCACCCCCTTAGGGGCCCAACGTCTTTGTTGGCACACCGCCTAGTGTCTAGGTCTGAGTATCATATTTGCTTTCTGCATGTACACCATAAATGTCTAGAATATCCTATTTGTGAGTAATTTAAACTTTGTACCTGCAAGTTTTCTAAACCAAATGTAGTATGCACTCATTTATCCTTTGCATCTAGCTTTGACTCTACCTCAAATAAACTTTTTCTCATCTTTTTGTTGCAGATGTGGTCATGAATTTTGCTATAAATGTGGAGGAGAATGGAAAGATAAAAAGGCGACATGTTCTTGTCCGCTATGGGCTGAAAATCATATTTTGTACAACGATGATAATAGAGAATTCAATCATGACGGTCGATTCTACGAAGAAGAAGATGAAGAAGAAGAGCTTTATGATTCAGATGACGAGTTTTACTATATCTGATAAACAACAAAACATCTTCCTTCTAGCTCACTTTCAATATCACTCAAATAATCCATCTTGGGATTTGATGTTTTGCGTGTAAATTGCCTGTTCGTAGTTTTAACATATTTGAACTTAGAAATGATTCAAATGTTCAGAAGTAACTTTTGTGTTTGAACTTCTCTTTTTGGATCTCCCCTTTTGTAGCAGGTTTTAGTCAATGAAGTTTGCTCTTTCTAGTTCTACAGCCC

mRNA sequence

ACTAAACCCTCTCCAAATCCGTCGGCTAAGACTTCATAGTTTCCGAGCAGCTTCGTGATTCCATGGACGCCTTAGGATTCGAATCGGACTTCGAATCGGACCTCGACCTTGCCTACAACCTCCAGCTCGAGGAAGCTCTCGCCGCATCACTCGCTTCATCCTCCTCCTCCGCCTCAATTCCTCGGCCGGGATTTCAAGAATTCGAACGCGATGATATCACTAGAACCGCCACACTCCACTCCAGGGACATCGAGAATGACGATCAGATATTCCAAGATTGGTTGCAATCGGAGTTTGAGATGCGCAGGACTGTCGGGGAAGTTGATCGTCGGGTTCGTAATCACGGTTTCGAGAGGGAGATTCTAAACATTTCCGATGATGACTGTGACGAGTGGGGTGATACTTCTCAGAATCACTTCTTCGGCGAGGGATGCTCGAAGGGCGTTGAGAATCAGGGCGTTTTTAAGCTCTATTTCAAGGGTTTGGTGAGTGAGGAAGGGATTGGAATTGAGAGACGTTTGGTAGCTGGAATCGGTGTTGCTATTTGCAACCCGGAGGGCGAATTAGTGTTCGAGGTGAAAAAACACCTTGTAGGGAATGAAAGGAGTAAAATCGTGGCTGAGTTTAAGGCTTTGATCACAGGACTGGACGCAGCTATGGATTTGAAATTGAAGCGCCTTTGTTTTTACTGCGACTACTACCCTCTTTATCAATTTGTTAGTGGCAAATGGCCACCGAAGCAGCGCAAGATTGTCGCTCTACTCGATCGGGTAACTCATCTGCAACAAGCACATTTTGACTCTTGCTATCCCAGGCTTGTGGCTAGGCATGATATCAAATTTGCGTTCAAACTAGCGAGAGAGGCAATAGTTCCTCAGATCACACGACCTGTTGATCCTGCCCCAAAGAAGAAGCTACATGAGACTTGTATTATTTGTTTGGAGGAGTGTGATTTTAATAGGATGTTTGCAGTCGATGGCTGCTCGCATCGGTACTGCTCTTCTTGTATGAAACAACATGTGGAAGTGAAATTACTTCAAGGATTGGTGCCTAAGTGTCCGCATGATGGGTGTAAGTTTGACCTTAATGTCGATAGCTGTGCAAAATTCTTGACACCTAAAGATATGGTTACGATGCGCCAACGTATTAAAGAAGCTTCCATACCCGTTTCTGAGAAAGTTTATTGTCCATATCCAAGATGTTCGGCTCTAATGACAAAAGTCGAGGTCTTGGAATACACTAGAGACATCTTTAGTGGTGCCAACCAATCTGGAATGCGGAAATGCATGAAGTGTCATGGTCTTTTCTGTGTCAACTGCAAGGTTCCTTGGCATAATCGTGTTTCCTGCAATGACTACAAAAGGTCAAATAATCTTCCCACTGATGATGTGAAATTGAATTACCTGGCTTCAACATGTCTGTGGCGCCAATGTGTGAAGTGTAATCACATGATTGAACTTGCTGAAGGTTGCTATCACATGACTTGCAGATGTGGTCATGAATTTTGCTATAAATGTGGAGGAGAATGGAAAGATAAAAAGGCGACATGTTCTTGTCCGCTATGGGCTGAAAATCATATTTTGTACAACGATGATAATAGAGAATTCAATCATGACGGTCGATTCTACGAAGAAGAAGATGAAGAAGAAGAGCTTTATGATTCAGATGACGAGTTTTACTATATCTGATAAACAACAAAACATCTTCCTTCTAGCTCACTTTCAATATCACTCAAATAATCCATCTTGGGATTTGATGTTTTGCGTGTAAATTGCCTGTTCGTAGTTTTAACATATTTGAACTTAGAAATGATTCAAATGTTCAGAAGTAACTTTTGTGTTTGAACTTCTCTTTTTGGATCTCCCCTTTTGTAGCAGGTTTTAGTCAATGAAGTTTGCTCTTTCTAGTTCTACAGCCC

Coding sequence (CDS)

ATGGACGCCTTAGGATTCGAATCGGACTTCGAATCGGACCTCGACCTTGCCTACAACCTCCAGCTCGAGGAAGCTCTCGCCGCATCACTCGCTTCATCCTCCTCCTCCGCCTCAATTCCTCGGCCGGGATTTCAAGAATTCGAACGCGATGATATCACTAGAACCGCCACACTCCACTCCAGGGACATCGAGAATGACGATCAGATATTCCAAGATTGGTTGCAATCGGAGTTTGAGATGCGCAGGACTGTCGGGGAAGTTGATCGTCGGGTTCGTAATCACGGTTTCGAGAGGGAGATTCTAAACATTTCCGATGATGACTGTGACGAGTGGGGTGATACTTCTCAGAATCACTTCTTCGGCGAGGGATGCTCGAAGGGCGTTGAGAATCAGGGCGTTTTTAAGCTCTATTTCAAGGGTTTGGTGAGTGAGGAAGGGATTGGAATTGAGAGACGTTTGGTAGCTGGAATCGGTGTTGCTATTTGCAACCCGGAGGGCGAATTAGTGTTCGAGGTGAAAAAACACCTTGTAGGGAATGAAAGGAGTAAAATCGTGGCTGAGTTTAAGGCTTTGATCACAGGACTGGACGCAGCTATGGATTTGAAATTGAAGCGCCTTTGTTTTTACTGCGACTACTACCCTCTTTATCAATTTGTTAGTGGCAAATGGCCACCGAAGCAGCGCAAGATTGTCGCTCTACTCGATCGGGTAACTCATCTGCAACAAGCACATTTTGACTCTTGCTATCCCAGGCTTGTGGCTAGGCATGATATCAAATTTGCGTTCAAACTAGCGAGAGAGGCAATAGTTCCTCAGATCACACGACCTGTTGATCCTGCCCCAAAGAAGAAGCTACATGAGACTTGTATTATTTGTTTGGAGGAGTGTGATTTTAATAGGATGTTTGCAGTCGATGGCTGCTCGCATCGGTACTGCTCTTCTTGTATGAAACAACATGTGGAAGTGAAATTACTTCAAGGATTGGTGCCTAAGTGTCCGCATGATGGGTGTAAGTTTGACCTTAATGTCGATAGCTGTGCAAAATTCTTGACACCTAAAGATATGGTTACGATGCGCCAACGTATTAAAGAAGCTTCCATACCCGTTTCTGAGAAAGTTTATTGTCCATATCCAAGATGTTCGGCTCTAATGACAAAAGTCGAGGTCTTGGAATACACTAGAGACATCTTTAGTGGTGCCAACCAATCTGGAATGCGGAAATGCATGAAGTGTCATGGTCTTTTCTGTGTCAACTGCAAGGTTCCTTGGCATAATCGTGTTTCCTGCAATGACTACAAAAGGTCAAATAATCTTCCCACTGATGATGTGAAATTGAATTACCTGGCTTCAACATGTCTGTGGCGCCAATGTGTGAAGTGTAATCACATGATTGAACTTGCTGAAGGTTGCTATCACATGACTTGCAGATGTGGTCATGAATTTTGCTATAAATGTGGAGGAGAATGGAAAGATAAAAAGGCGACATGTTCTTGTCCGCTATGGGCTGAAAATCATATTTTGTACAACGATGATAATAGAGAATTCAATCATGACGGTCGATTCTACGAAGAAGAAGATGAAGAAGAAGAGCTTTATGATTCAGATGACGAGTTTTACTATATCTGA

Protein sequence

MDALGFESDFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHSRDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFFGEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNERSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCKVPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYYI
Homology
BLAST of CmoCh20G005030 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.1e-20
Identity = 69/254 (27.17%), Postives = 114/254 (44.88%), Query Frame = 0

Query: 263 KLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNRMFAVDGCSHRYCSSCMKQHVEV 322
           KL  +A VP + +      +K  + +C+ICLE+    + FA+  C+HRYC  C K ++E+
Sbjct: 113 KLCIDAGVPNVMKLNATIVEKSGNVSCLICLEDYPPTQTFALI-CNHRYCLPCYKNYLEI 172

Query: 323 KLLQG---LVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQRIKEASIPVSEKV-YCPYP 382
           K+ +G   +   CP   CK  ++ D+  + ++P+        I ++ +  + +V +CP P
Sbjct: 173 KVSEGPECIYTPCPAPKCKVIVHQDAFKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAP 232

Query: 383 RCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCKVPWHNRVSCNDYKRSNNL 442
            C               I+S        +C +      VNCK  +    +CNDY+  +++
Sbjct: 233 GC---------------IYS-------IRCDRKERKEAVNCKCGFQYCFNCNDYEIGDHM 292

Query: 443 P------------TDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCR-----CGHEF 496
           P              D   N        ++C +C   IE   GC HMTCR     CG EF
Sbjct: 293 PCPCSQVDKWLQKASDESENVTWMLANTKKCPECRSPIEKNGGCMHMTCRKNAGGCGFEF 343

BLAST of CmoCh20G005030 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 87.0 bits (214), Expect = 6.9e-16
Identity = 59/209 (28.23%), Postives = 90/209 (43.06%), Query Frame = 0

Query: 289 CIICLEECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVP-KCPHDGCKFDLNVDSCA 348
           C +CLE+     +  +  C    C  C+K ++  ++  G V  KCP   C   L   +  
Sbjct: 263 CRVCLED---KPIKPLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFLEETTVV 322

Query: 349 KFLTPKDMVTMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRK 408
             LT +D +  +  ++   I  S K   P P+C    T  +         S +      +
Sbjct: 323 YNLTHEDSIKYKYFLELGRIDSSTK---PCPQCKHFTTFKKKGHIPTP--SRSESKYKIQ 382

Query: 409 CMKCHGLFCVNCKVPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQ-----CVKCNH 468
           C  C  ++C  C  PWH  V+C +YK+ + L      L + AS     Q     C KC  
Sbjct: 383 CPTCQFVWCFKCHSPWHEGVNCKEYKKGDKL------LRHWASEIEHGQRNAQKCPKCKI 442

Query: 469 MIELAEGCYHMTC-RCGHEFCYKCGGEWK 491
            I+  EGC HMTC +C   FCY+CG  ++
Sbjct: 443 HIQRTEGCDHMTCSQCNTNFCYRCGERYR 457

BLAST of CmoCh20G005030 vs. ExPASy Swiss-Prot
Match: A2VEA3 (E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.5e-15
Identity = 75/303 (24.75%), Postives = 128/303 (42.24%), Query Frame = 0

Query: 237 VTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHE--------- 296
           +T +  +HF+    +L+ R+      KL  E  V      ++P+ K +  +         
Sbjct: 128 ITRILLSHFNWDKEKLMERYFDGNLEKLFAECHV------INPSKKSRTRQMNTRSSAQD 187

Query: 297 -TCIICLEECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQ---GLVPKCPHDGCKFDLNV 356
             C IC      N  F    C H++C  C  +++  K+++   G    CP  GC   ++ 
Sbjct: 188 MPCQICYLNYP-NSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDD 247

Query: 357 DSCAKFLTPKDMVTMRQRIKEASIPVSEKV--YCPYPRCSALMTKVEVLEYTRDIFSGAN 416
           ++  + +T   +    Q +   S     ++  +CP P C  ++ KV+  +          
Sbjct: 248 NTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVV-KVQYPD---------- 307

Query: 417 QSGMRKCMKCHGLFCVNCKVPWHNRVSCNDYKRSNNLPTDDVKL-NYLASTCLWRQCVKC 476
            +   +C KC   FC NC   WH+ V C   K+      DD +  N++A+    ++C KC
Sbjct: 308 -AKPVRC-KCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANT--KECPKC 367

Query: 477 NHMIELAEGCYHMTCR---CGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNH 521
           +  IE   GC HM CR   C  EFC+ C G W+   +      W  N   YN+D+ +   
Sbjct: 368 HVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSA-----W-YNCNRYNEDDAKAAR 402

BLAST of CmoCh20G005030 vs. ExPASy Swiss-Prot
Match: Q9Y4X5 (E3 ubiquitin-protein ligase ARIH1 OS=Homo sapiens OX=9606 GN=ARIH1 PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 1.5e-15
Identity = 75/303 (24.75%), Postives = 128/303 (42.24%), Query Frame = 0

Query: 237 VTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHE--------- 296
           +T +  +HF+    +L+ R+      KL  E  V      ++P+ K +  +         
Sbjct: 130 ITRILLSHFNWDKEKLMERYFDGNLEKLFAECHV------INPSKKSRTRQMNTRSSAQD 189

Query: 297 -TCIICLEECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQ---GLVPKCPHDGCKFDLNV 356
             C IC      N  F    C H++C  C  +++  K+++   G    CP  GC   ++ 
Sbjct: 190 MPCQICYLNYP-NSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDD 249

Query: 357 DSCAKFLTPKDMVTMRQRIKEASIPVSEKV--YCPYPRCSALMTKVEVLEYTRDIFSGAN 416
           ++  + +T   +    Q +   S     ++  +CP P C  ++ KV+  +          
Sbjct: 250 NTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVV-KVQYPD---------- 309

Query: 417 QSGMRKCMKCHGLFCVNCKVPWHNRVSCNDYKRSNNLPTDDVKL-NYLASTCLWRQCVKC 476
            +   +C KC   FC NC   WH+ V C   K+      DD +  N++A+    ++C KC
Sbjct: 310 -AKPVRC-KCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANT--KECPKC 369

Query: 477 NHMIELAEGCYHMTCR---CGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNH 521
           +  IE   GC HM CR   C  EFC+ C G W+   +      W  N   YN+D+ +   
Sbjct: 370 HVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSA-----W-YNCNRYNEDDAKAAR 404

BLAST of CmoCh20G005030 vs. ExPASy Swiss-Prot
Match: Q9Z1K5 (E3 ubiquitin-protein ligase ARIH1 OS=Mus musculus OX=10090 GN=Arih1 PE=1 SV=3)

HSP 1 Score: 85.9 bits (211), Expect = 1.5e-15
Identity = 75/303 (24.75%), Postives = 128/303 (42.24%), Query Frame = 0

Query: 237 VTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHE--------- 296
           +T +  +HF+    +L+ R+      KL  E  V      ++P+ K +  +         
Sbjct: 128 ITRILLSHFNWDKEKLMERYFDGNLEKLFAECHV------INPSKKSRTRQMNTRSSAQD 187

Query: 297 -TCIICLEECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQ---GLVPKCPHDGCKFDLNV 356
             C IC      N  F    C H++C  C  +++  K+++   G    CP  GC   ++ 
Sbjct: 188 MPCQICYLNYP-NSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDD 247

Query: 357 DSCAKFLTPKDMVTMRQRIKEASIPVSEKV--YCPYPRCSALMTKVEVLEYTRDIFSGAN 416
           ++  + +T   +    Q +   S     ++  +CP P C  ++ KV+  +          
Sbjct: 248 NTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVV-KVQYPD---------- 307

Query: 417 QSGMRKCMKCHGLFCVNCKVPWHNRVSCNDYKRSNNLPTDDVKL-NYLASTCLWRQCVKC 476
            +   +C KC   FC NC   WH+ V C   K+      DD +  N++A+    ++C KC
Sbjct: 308 -AKPVRC-KCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANT--KECPKC 367

Query: 477 NHMIELAEGCYHMTCR---CGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNH 521
           +  IE   GC HM CR   C  EFC+ C G W+   +      W  N   YN+D+ +   
Sbjct: 368 HVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSA-----W-YNCNRYNEDDAKAAR 402

BLAST of CmoCh20G005030 vs. ExPASy TrEMBL
Match: A0A6J1FUK7 (uncharacterized protein LOC111448273 OS=Cucurbita moschata OX=3662 GN=LOC111448273 PE=3 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 541/541 (100.00%), Postives = 541/541 (100.00%), Query Frame = 0

Query: 1   MDALGFESDFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS 60
           MDALGFESDFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS
Sbjct: 1   MDALGFESDFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS 60

Query: 61  RDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFF 120
           RDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFF
Sbjct: 61  RDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFF 120

Query: 121 GEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNE 180
           GEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNE
Sbjct: 121 GEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNE 180

Query: 181 RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHL 240
           RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHL
Sbjct: 181 RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHL 240

Query: 241 QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNR 300
           QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNR
Sbjct: 241 QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNR 300

Query: 301 MFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQ 360
           MFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQ
Sbjct: 301 MFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQ 360

Query: 361 RIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCK 420
           RIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCK
Sbjct: 361 RIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCK 420

Query: 421 VPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHE 480
           VPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHE
Sbjct: 421 VPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHE 480

Query: 481 FCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYY 540
           FCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYY
Sbjct: 481 FCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYY 540

Query: 541 I 542
           I
Sbjct: 541 I 541

BLAST of CmoCh20G005030 vs. ExPASy TrEMBL
Match: A0A6J1J8H4 (uncharacterized protein LOC111483577 OS=Cucurbita maxima OX=3661 GN=LOC111483577 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 515/531 (96.99%), Postives = 521/531 (98.12%), Query Frame = 0

Query: 11  ESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHSRDIENDDQIF 70
           ESDLDLAYNLQLEEALAASLASSSSSAS PRPGFQEFERDDI+RTATLHSRDIENDDQIF
Sbjct: 7   ESDLDLAYNLQLEEALAASLASSSSSASNPRPGFQEFERDDISRTATLHSRDIENDDQIF 66

Query: 71  QDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFFGEGCSKGVEN 130
           QDWLQSEFEMRRTVGEVDRRVRNHGF REILNISDDDCDEWGDTSQNHFFGEGCSKGVEN
Sbjct: 67  QDWLQSEFEMRRTVGEVDRRVRNHGFAREILNISDDDCDEWGDTSQNHFFGEGCSKGVEN 126

Query: 131 QGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNERSKIVAEFKA 190
           QGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPE ELVFEVKKHL GNERSKIVAEFKA
Sbjct: 127 QGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEDELVFEVKKHLAGNERSKIVAEFKA 186

Query: 191 LITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHLQQAHFDSCYP 250
           LITGLDAAMDL LKRLCFYCDYYPLYQFVSGKWPPKQRKIV LLDRVTHLQQAHFDSCYP
Sbjct: 187 LITGLDAAMDLNLKRLCFYCDYYPLYQFVSGKWPPKQRKIVTLLDRVTHLQQAHFDSCYP 246

Query: 251 RLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNRMFAVDGCSHR 310
           RLVARHDIKFAFKLAREAIVPQ TRPVDPAPKKKLHETC+ICLEECDFNRMFAVDGCSHR
Sbjct: 247 RLVARHDIKFAFKLAREAIVPQTTRPVDPAPKKKLHETCVICLEECDFNRMFAVDGCSHR 306

Query: 311 YCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQRIKEASIPVS 370
           YCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDM TMRQRIKEASIPVS
Sbjct: 307 YCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMTTMRQRIKEASIPVS 366

Query: 371 EKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCKVPWHNRVSCN 430
           EKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFC+NCKVPWHNRVSCN
Sbjct: 367 EKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCINCKVPWHNRVSCN 426

Query: 431 DYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWK 490
           DYKRSNNLPTDDVKL  LASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWK
Sbjct: 427 DYKRSNNLPTDDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWK 486

Query: 491 DKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYYI 542
           DKKATCSCPLWAENHILYNDDNREF+HDG+FY+EEDEEEELYDSDDEFYYI
Sbjct: 487 DKKATCSCPLWAENHILYNDDNREFDHDGQFYDEEDEEEELYDSDDEFYYI 537

BLAST of CmoCh20G005030 vs. ExPASy TrEMBL
Match: A0A1S3CK99 (uncharacterized protein LOC103501921 OS=Cucumis melo OX=3656 GN=LOC103501921 PE=3 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.8e-248
Identity = 413/532 (77.63%), Postives = 470/532 (88.35%), Query Frame = 0

Query: 11  ESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHSRDIENDDQIF 70
           ESDL+LAYNLQLEEAL ASLASSSSS+SIPRP  Q+FER  I R  TLHSRDIE  D+IF
Sbjct: 32  ESDLELAYNLQLEEALVASLASSSSSSSIPRPEVQDFERVGIPRIGTLHSRDIEKCDRIF 91

Query: 71  QDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFFGEGCSKGVEN 130
           QDWLQ+EF+MRRT GE  R+ +NHGF REILNI DD+  +  DTS+   FGEGCS GVE+
Sbjct: 92  QDWLQTEFDMRRTGGERHRQGQNHGFAREILNIHDDNWRDQSDTSRKP-FGEGCSNGVED 151

Query: 131 QGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNERSKIVAEFKA 190
           + VFKLYFKGLVSEE IG E+R+VAGIGVAICNPE +LV EVK+ L GNERSKIVAE KA
Sbjct: 152 REVFKLYFKGLVSEEEIGNEKRVVAGIGVAICNPEDKLVVEVKRRLAGNERSKIVAELKA 211

Query: 191 LITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHLQQAHFDSCYP 250
           LI GL+ AMDLKLKRLCFYCDYYPL+QF++G+WPPKQRK+ ALL ++ HL +  FDSC  
Sbjct: 212 LIAGLNVAMDLKLKRLCFYCDYYPLFQFITGRWPPKQRKVAALLSQLAHL-RVRFDSCSH 271

Query: 251 RLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNRMFAVDGCSHR 310
             VAR+DIK+AFKLAR+AI PQ+T+   PAPKKKL+ETC+ICLE+CD +RMFAVDGCSHR
Sbjct: 272 VHVARNDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMFAVDGCSHR 331

Query: 311 YCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQRIKEASIPVS 370
           YC SCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDM T+RQRIKEASIPVS
Sbjct: 332 YCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRIKEASIPVS 391

Query: 371 EKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCKVPWHNRVSCN 430
           EKVYCPYPRCSALMTKVEVL YT+D+FS ANQSG+RKCMKCHGLFC+NCKVPWHNR++CN
Sbjct: 392 EKVYCPYPRCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCINCKVPWHNRITCN 451

Query: 431 DYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWK 490
           DYKRSNNLPT+DVKL  LASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCG EWK
Sbjct: 452 DYKRSNNLPTEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGAEWK 511

Query: 491 DKKATCSCPLWAENHILY-NDDNREFNHDGRFYEEEDEEEELYDSDDEFYYI 542
           +KKATCSCPLWAENHI + +DD+R+ +HD ++Y++ED EEELYDS+DE++ +
Sbjct: 512 NKKATCSCPLWAENHIWHDDDDDRDSDHDDQYYDDED-EEELYDSEDEYFIL 560

BLAST of CmoCh20G005030 vs. ExPASy TrEMBL
Match: A0A0A0KEP0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290800 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.1e-247
Identity = 409/533 (76.74%), Postives = 463/533 (86.87%), Query Frame = 0

Query: 11  ESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHSRDIENDDQIF 70
           ESDLDLAYNLQLEEAL ASLASSSSS+SI RP  Q FE  D  R  TLHSRD+E  D+IF
Sbjct: 32  ESDLDLAYNLQLEEALVASLASSSSSSSIHRPEVQNFEHVDRPRIGTLHSRDVEECDRIF 91

Query: 71  QDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHF--FGEGCSKGV 130
           QDWLQ+EF+MRRT GE  R+V NHG  R ILNI DDD   W D S+N    FGEGCS GV
Sbjct: 92  QDWLQTEFDMRRTGGERHRQVHNHGCARGILNIRDDD---WRDQSENSRKPFGEGCSNGV 151

Query: 131 ENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNERSKIVAEF 190
           ++QGVFKLYFKGLV EE IG E+R+VAGIGVAICNPE +LV EVK+ L GNERSKIVAE 
Sbjct: 152 DDQGVFKLYFKGLVGEEEIGNEKRVVAGIGVAICNPEDKLVVEVKRRLPGNERSKIVAEL 211

Query: 191 KALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHLQQAHFDSC 250
           KALI G + A+DLKLKRLCFY DYYPL+QF++G+WPPKQRK+ ALL ++ HL +  FDSC
Sbjct: 212 KALIAGFNVALDLKLKRLCFYSDYYPLFQFITGRWPPKQRKVAALLSQLAHL-RVRFDSC 271

Query: 251 YPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNRMFAVDGCS 310
               VARHDIK+AFKLAR+AI PQ+T+   PAPKKKL+ETC+ICLE+CD +RMFAVDGCS
Sbjct: 272 THVHVARHDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMFAVDGCS 331

Query: 311 HRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQRIKEASIP 370
           HRYC SCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDM TMRQRIKEASIP
Sbjct: 332 HRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATMRQRIKEASIP 391

Query: 371 VSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCKVPWHNRVS 430
           VSEKVYCPYPRCSALM KVEVL YT+D+F  ANQSG+RKCMKCHGLFC++CKVPWHNR++
Sbjct: 392 VSEKVYCPYPRCSALMRKVEVLAYTKDVFGTANQSGVRKCMKCHGLFCIDCKVPWHNRIT 451

Query: 431 CNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGE 490
           CNDYKRSNNLPT+DVKL  LASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCG E
Sbjct: 452 CNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGAE 511

Query: 491 WKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYYI 542
           WK+KKATCSCPLWAENHI ++DD+R+F+HD R+Y++E++EEELYDS+DE++ +
Sbjct: 512 WKNKKATCSCPLWAENHIWHDDDDRDFDHDDRYYDDEEDEEELYDSEDEYFIL 560

BLAST of CmoCh20G005030 vs. ExPASy TrEMBL
Match: A0A6J1CGV8 (uncharacterized protein LOC111011310 OS=Momordica charantia OX=3673 GN=LOC111011310 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 4.1e-245
Identity = 423/545 (77.61%), Postives = 461/545 (84.59%), Query Frame = 0

Query: 1   MDALGFESDFESDLDLAYNLQLEEALAASLAS---SSSSASIPRPGFQEFER-DDITRTA 60
           MDAL      +SDLD AY+LQLEEALAASLAS   +SSS+SI R   QEFER DD +   
Sbjct: 29  MDALA----LDSDLDFAYHLQLEEALAASLASQAPASSSSSILRSELQEFERDDDFSGIG 88

Query: 61  TLHSRDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQ 120
           +LH++D+   DQIFQDWLQ +FEMRRT GE+ RR+ +HGF REI NI DDD  E GDT Q
Sbjct: 89  SLHAQDVAKSDQIFQDWLQCQFEMRRTGGELHRRIHDHGFAREIFNIRDDDWSECGDTFQ 148

Query: 121 NHFFGEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHL 180
              FGEG SKGVENQG   LYFKGLVSEEGIG ERR V GIGVAIC+PE +LVFE+KK L
Sbjct: 149 KP-FGEGSSKGVENQGFSSLYFKGLVSEEGIGNERRAVVGIGVAICDPEDKLVFEMKKPL 208

Query: 181 VGNERSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDR 240
           +GNERSKIVAE KALI GL+AAM LKLKRL FYCDYYPLYQFV+GKWPPKQRKI  LL +
Sbjct: 209 IGNERSKIVAEVKALIEGLNAAMGLKLKRLRFYCDYYPLYQFVTGKWPPKQRKIAVLLSQ 268

Query: 241 VTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEEC 300
           VTHLQ   FDSC P+LVARHDIKFAFKLAR+AIV +ITR   PA KKKL+E C+ICLE+C
Sbjct: 269 VTHLQIC-FDSCKPKLVARHDIKFAFKLARDAIVTEITRTEGPALKKKLNEACVICLEDC 328

Query: 301 DFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMV 360
           D NRMFAVDGC HRYC SCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDM 
Sbjct: 329 DVNRMFAVDGCLHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMA 388

Query: 361 TMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFC 420
           TMRQRIKEASIPVSEKVYCPYP+CSALMTK EVLEYTRD+  GANQSG+RKCMKCHGLFC
Sbjct: 389 TMRQRIKEASIPVSEKVYCPYPKCSALMTKDEVLEYTRDVLGGANQSGVRKCMKCHGLFC 448

Query: 421 VNCKVPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCR 480
           +NCKVPWHNRVSCNDYKRSNN  ++DVKL  LAST LWRQCVKCNHMIELAEGCYHMTCR
Sbjct: 449 INCKVPWHNRVSCNDYKRSNNPASEDVKLKSLASTNLWRQCVKCNHMIELAEGCYHMTCR 508

Query: 481 CGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDD 540
           CGHEFCYKCGGEWKDKKATCSCPLWAE+ I YND+NR+F+ D  + +E  EEEE YDSDD
Sbjct: 509 CGHEFCYKCGGEWKDKKATCSCPLWAEDRIWYNDNNRDFDDDDIYTDE--EEEEFYDSDD 565

Query: 541 EFYYI 542
           E YYI
Sbjct: 569 ECYYI 565

BLAST of CmoCh20G005030 vs. NCBI nr
Match: XP_022943524.1 (uncharacterized protein LOC111448273 [Cucurbita moschata])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 541/541 (100.00%), Postives = 541/541 (100.00%), Query Frame = 0

Query: 1   MDALGFESDFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS 60
           MDALGFESDFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS
Sbjct: 1   MDALGFESDFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS 60

Query: 61  RDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFF 120
           RDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFF
Sbjct: 61  RDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFF 120

Query: 121 GEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNE 180
           GEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNE
Sbjct: 121 GEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNE 180

Query: 181 RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHL 240
           RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHL
Sbjct: 181 RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHL 240

Query: 241 QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNR 300
           QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNR
Sbjct: 241 QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNR 300

Query: 301 MFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQ 360
           MFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQ
Sbjct: 301 MFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQ 360

Query: 361 RIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCK 420
           RIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCK
Sbjct: 361 RIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCK 420

Query: 421 VPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHE 480
           VPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHE
Sbjct: 421 VPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHE 480

Query: 481 FCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYY 540
           FCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYY
Sbjct: 481 FCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYY 540

Query: 541 I 542
           I
Sbjct: 541 I 541

BLAST of CmoCh20G005030 vs. NCBI nr
Match: XP_023511953.1 (uncharacterized protein LOC111776808 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 528/547 (96.53%), Postives = 536/547 (97.99%), Query Frame = 0

Query: 1   MDALGFESD------FESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITR 60
           MDALGFESD      FESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDI+R
Sbjct: 1   MDALGFESDPMDALGFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDISR 60

Query: 61  TATLHSRDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDT 120
           TA++HSRDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGF REILNISDDDCDEWGDT
Sbjct: 61  TASIHSRDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFAREILNISDDDCDEWGDT 120

Query: 121 SQNHFFGEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKK 180
           SQNHFFGEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPE ELVFEVKK
Sbjct: 121 SQNHFFGEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEDELVFEVKK 180

Query: 181 HLVGNERSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALL 240
           HLVGNERSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALL
Sbjct: 181 HLVGNERSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALL 240

Query: 241 DRVTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLE 300
           DRVTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETC+ICLE
Sbjct: 241 DRVTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCVICLE 300

Query: 301 ECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKD 360
           ECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKD
Sbjct: 301 ECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKD 360

Query: 361 MVTMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGL 420
           M TMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGL
Sbjct: 361 MATMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGL 420

Query: 421 FCVNCKVPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMT 480
           FC+NCKVPWHNRVSCNDYKRSNNLPTDDVKL  LASTCLWRQCVKCNHMIELAEGCYHMT
Sbjct: 421 FCINCKVPWHNRVSCNDYKRSNNLPTDDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMT 480

Query: 481 CRCGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDS 540
           CRCGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREF+HDGRFY+EEDEE+ELYDS
Sbjct: 481 CRCGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFHHDGRFYDEEDEEDELYDS 540

Query: 541 DDEFYYI 542
           DDEFYYI
Sbjct: 541 DDEFYYI 547

BLAST of CmoCh20G005030 vs. NCBI nr
Match: KAG6570763.1 (putative E3 ubiquitin-protein ligase rbrA, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 526/541 (97.23%), Postives = 530/541 (97.97%), Query Frame = 0

Query: 1   MDALGFESDFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS 60
           MDA GFESD ESD DLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS
Sbjct: 1   MDASGFESDLESDHDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS 60

Query: 61  RDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFF 120
            DIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGF REI NISDDDCDEWGDTSQNHFF
Sbjct: 61  WDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFAREIPNISDDDCDEWGDTSQNHFF 120

Query: 121 GEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNE 180
           GEGCSKGV NQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPE ELVFEVKKHLVGNE
Sbjct: 121 GEGCSKGVANQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEDELVFEVKKHLVGNE 180

Query: 181 RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHL 240
           RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHL
Sbjct: 181 RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHL 240

Query: 241 QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNR 300
           QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETC+ICLEECDFNR
Sbjct: 241 QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCVICLEECDFNR 300

Query: 301 MFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQ 360
           MFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDM TMRQ
Sbjct: 301 MFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATMRQ 360

Query: 361 RIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCK 420
           RIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFC+NCK
Sbjct: 361 RIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCINCK 420

Query: 421 VPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHE 480
           VPWHNRVSCNDYKRSNNLPTDDVKL  LASTCLWRQCVKCNHMIELAEGCYHMTCRCGHE
Sbjct: 421 VPWHNRVSCNDYKRSNNLPTDDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHE 480

Query: 481 FCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYY 540
           FCYKCGGEWKDKKATCSCPLWAENHILYNDDNREF+HDGRFY+EEDEEEELYDSDDEFYY
Sbjct: 481 FCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFDHDGRFYDEEDEEEELYDSDDEFYY 540

Query: 541 I 542
           I
Sbjct: 541 I 541

BLAST of CmoCh20G005030 vs. NCBI nr
Match: KAG7010609.1 (putative E3 ubiquitin-protein ligase rbrA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 526/554 (94.95%), Postives = 530/554 (95.67%), Query Frame = 0

Query: 1   MDALGFESDFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS 60
           MDA GFESD ESD DLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS
Sbjct: 1   MDASGFESDLESDHDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHS 60

Query: 61  RDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFF 120
            DIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGF REI NISDDDCDEWGDTSQNHFF
Sbjct: 61  WDIENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFAREIPNISDDDCDEWGDTSQNHFF 120

Query: 121 GEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNE 180
           GEGCSKGV NQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPE ELVFEVKKHLVGNE
Sbjct: 121 GEGCSKGVANQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEDELVFEVKKHLVGNE 180

Query: 181 RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQF-------------VSGKWPPKQ 240
           RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQF             VSGKWPPKQ
Sbjct: 181 RSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFNYNKQSYYLSREQVSGKWPPKQ 240

Query: 241 RKIVALLDRVTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHE 300
           RKIVALLDRVTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHE
Sbjct: 241 RKIVALLDRVTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHE 300

Query: 301 TCIICLEECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCA 360
           TC+ICLEECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCA
Sbjct: 301 TCVICLEECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCA 360

Query: 361 KFLTPKDMVTMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRK 420
           KFLTPKDM TMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRK
Sbjct: 361 KFLTPKDMATMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRK 420

Query: 421 CMKCHGLFCVNCKVPWHNRVSCNDYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELA 480
           CMKCHGLFC+NCKVPWHNRVSCNDYKRSNNLPTDDVKL  LASTCLWRQCVKCNHMIELA
Sbjct: 421 CMKCHGLFCINCKVPWHNRVSCNDYKRSNNLPTDDVKLKSLASTCLWRQCVKCNHMIELA 480

Query: 481 EGCYHMTCRCGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDE 540
           EGCYHMTCRCGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREF+HDGRFY+EEDE
Sbjct: 481 EGCYHMTCRCGHEFCYKCGGEWKDKKATCSCPLWAENHILYNDDNREFDHDGRFYDEEDE 540

Query: 541 EEELYDSDDEFYYI 542
           EEELYDSDDEFYYI
Sbjct: 541 EEELYDSDDEFYYI 554

BLAST of CmoCh20G005030 vs. NCBI nr
Match: XP_022985536.1 (uncharacterized protein LOC111483577 [Cucurbita maxima])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 515/531 (96.99%), Postives = 521/531 (98.12%), Query Frame = 0

Query: 11  ESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHSRDIENDDQIF 70
           ESDLDLAYNLQLEEALAASLASSSSSAS PRPGFQEFERDDI+RTATLHSRDIENDDQIF
Sbjct: 7   ESDLDLAYNLQLEEALAASLASSSSSASNPRPGFQEFERDDISRTATLHSRDIENDDQIF 66

Query: 71  QDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFFGEGCSKGVEN 130
           QDWLQSEFEMRRTVGEVDRRVRNHGF REILNISDDDCDEWGDTSQNHFFGEGCSKGVEN
Sbjct: 67  QDWLQSEFEMRRTVGEVDRRVRNHGFAREILNISDDDCDEWGDTSQNHFFGEGCSKGVEN 126

Query: 131 QGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNERSKIVAEFKA 190
           QGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPE ELVFEVKKHL GNERSKIVAEFKA
Sbjct: 127 QGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEDELVFEVKKHLAGNERSKIVAEFKA 186

Query: 191 LITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHLQQAHFDSCYP 250
           LITGLDAAMDL LKRLCFYCDYYPLYQFVSGKWPPKQRKIV LLDRVTHLQQAHFDSCYP
Sbjct: 187 LITGLDAAMDLNLKRLCFYCDYYPLYQFVSGKWPPKQRKIVTLLDRVTHLQQAHFDSCYP 246

Query: 251 RLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNRMFAVDGCSHR 310
           RLVARHDIKFAFKLAREAIVPQ TRPVDPAPKKKLHETC+ICLEECDFNRMFAVDGCSHR
Sbjct: 247 RLVARHDIKFAFKLAREAIVPQTTRPVDPAPKKKLHETCVICLEECDFNRMFAVDGCSHR 306

Query: 311 YCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQRIKEASIPVS 370
           YCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDM TMRQRIKEASIPVS
Sbjct: 307 YCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMTTMRQRIKEASIPVS 366

Query: 371 EKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCKVPWHNRVSCN 430
           EKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFC+NCKVPWHNRVSCN
Sbjct: 367 EKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCINCKVPWHNRVSCN 426

Query: 431 DYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWK 490
           DYKRSNNLPTDDVKL  LASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWK
Sbjct: 427 DYKRSNNLPTDDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWK 486

Query: 491 DKKATCSCPLWAENHILYNDDNREFNHDGRFYEEEDEEEELYDSDDEFYYI 542
           DKKATCSCPLWAENHILYNDDNREF+HDG+FY+EEDEEEELYDSDDEFYYI
Sbjct: 487 DKKATCSCPLWAENHILYNDDNREFDHDGQFYDEEDEEEELYDSDDEFYYI 537

BLAST of CmoCh20G005030 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 440.3 bits (1131), Expect = 2.3e-123
Identity = 244/564 (43.26%), Postives = 334/564 (59.22%), Query Frame = 0

Query: 9   DFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHSRDIENDDQ 68
           DF+S  + A+ LQ+EEALAASL+S S +   P           +     L   +  ND +
Sbjct: 2   DFDS--EYAFRLQMEEALAASLSSQSRTPQRPP------SPPIVATCGVLIVENNPNDSK 61

Query: 69  IFQDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWG-----DTSQNH----- 128
             +      F+ +R VG  + + +    E      +D+     G      TS  H     
Sbjct: 62  TAKTSSGYGFDFKRAVGGGNSKAKGKTHESVTGVRTDERNPNIGLGNSRSTSTGHGNTKP 121

Query: 129 ----------FFGEGCSK------GVENQGVFKLYFKGLVSEE-GIGIERRLVAGIGVAI 188
                       GEG S+       +    +++LYFKGLVS+E G G    +V+G GVAI
Sbjct: 122 LPQETVRPAQLVGEGSSRVNTAAGKINGNLLYRLYFKGLVSDENGKGKMTDVVSGFGVAI 181

Query: 189 CNPEGELVFEVKKHLVGNERSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSG 248
           C+    L+FE+K  L+ N  S+  AE KALI GL  A+ L +K + F+CD YP++Q+V+G
Sbjct: 182 CDQRDNLLFEMKGPLIDNGMSRQGAELKALIRGLTEALKLGIKHIVFFCDSYPIFQYVTG 241

Query: 249 KWPPKQRKIVALLDRVTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAP 308
           KW  KQ+KI  LLD +  + Q HF S    LVAR+D+KFA+KLARE+I+  +T   DP  
Sbjct: 242 KWMAKQKKISLLLDDLQSIMQ-HFSSYQHVLVARNDVKFAYKLARESILSMVTPHEDPRQ 301

Query: 309 KKK-LHETCIICLEECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFD 368
            K  L E C IC  +     MF+VD C HR+C  C+KQHVEVKLL G+ PKCPHDGCK +
Sbjct: 302 AKAVLKEECAICFNDIVAEGMFSVDKCRHRFCFQCVKQHVEVKLLHGMAPKCPHDGCKSE 361

Query: 369 LNVDSCAKFLTPKDMVTMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGA 428
           L +D+C K LTPK     +QR++E +IPV+E+VYCPYPRCSALM+K ++ E  + + S  
Sbjct: 362 LVIDACGKLLTPKLSKLWQQRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLY 421

Query: 429 NQSGMRKCMKCHGLFCVNCKVPWHNRVSCNDYKRSN-NLPTDDVKLNYLASTCLWRQCVK 488
            +SG+R+C++C GLFCV+CKVPWH  +SC +YK+ +   P DDVKL  LA+  +WRQC K
Sbjct: 422 PKSGVRRCVECRGLFCVDCKVPWHGNLSCTEYKKLHPEPPADDVKLKSLANNKMWRQCGK 481

Query: 489 CNHMIELAEGCYHMTCRCGHEFCYKCGGEWKDKKATC--SCPLWAENHILYNDDNREFNH 540
           C HMIEL++GC H+TCRCGHEFCY CGG W     TC   CP W E +I   D  R    
Sbjct: 482 CQHMIELSQGCNHITCRCGHEFCYNCGGGWNKIMGTCLNRCPTWNEEYITRQDPARANVA 541

BLAST of CmoCh20G005030 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 2.2e-86
Identity = 171/379 (45.12%), Postives = 238/379 (62.80%), Query Frame = 0

Query: 121 GEGCSKGVE--NQGVFKLYFKGLVSEEGIG--IERRLVAGIGVAICNPEGELVFEVKKHL 180
           GEG S   +  +  V++L FKGLV+ E +   +E+ + AG GVAIC+ +  L+ E+K+ L
Sbjct: 139 GEGSSPAAKFMDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIKESL 198

Query: 181 VGNERSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDR 240
              E S+   E  AL+ GL  + DL ++ +  YCD   +YQ + G+   K +KI  L++ 
Sbjct: 199 RDIEISRRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSK-KKIDHLVEE 258

Query: 241 VTHL--QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLE 300
           V  +  + A  D+    LVAR+D+KFAF+LAREAI       VD   ++   ETC I  E
Sbjct: 259 VQGILEKMACIDAV---LVARNDVKFAFRLAREAIG---RNSVDVNAEQ--GETCGIFFE 318

Query: 301 ECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKD 360
           E D   MF  + C HR+C  C+KQHV+VKL  G  P C   GCKF L ++ C+K LT K 
Sbjct: 319 ETDVEHMFVTEKCLHRHCFPCVKQHVKVKLRSGTEPTCLEYGCKFKLTLERCSKVLTLKL 378

Query: 361 MVTMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGL 420
           +   +Q++KE SIP +E++YCPYP CS LM+K E       + S A+ S +R C+KC GL
Sbjct: 379 IEMWKQKMKEDSIPAAERIYCPYPNCSMLMSKTE-------LSSEADLSNVRTCVKCCGL 438

Query: 421 FCVNCKVPWHNRVSCNDYKRSNNLP-TDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHM 480
           FC++CKVP H  +S +DYK+ +  P  DD+KL  LA+  +WRQCVKC HMIEL+ GC HM
Sbjct: 439 FCIDCKVPSHTDLSYDDYKKLHPDPLVDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHM 498

Query: 481 TCRCGHEFCYKCGGEWKDK 493
           TCRCG+EFCY+CG EW+ +
Sbjct: 499 TCRCGYEFCYECGIEWQKR 501

BLAST of CmoCh20G005030 vs. TAIR 10
Match: AT3G45580.1 (RING/U-box protein with C6HC-type zinc finger )

HSP 1 Score: 313.9 bits (803), Expect = 2.5e-85
Identity = 155/366 (42.35%), Postives = 224/366 (61.20%), Query Frame = 0

Query: 134 FKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNERSKIVAEFKALIT 193
           ++LYFKGLVSEE +     L+AG GVAIC+ +  L+F++K+ +  +  + +  E  AL  
Sbjct: 28  YRLYFKGLVSEETV----ELLAGFGVAICDKDDNLLFQMKEQVHDSRVTVLEVEIMALKR 87

Query: 194 GLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHLQQAHFDSCYPRLV 253
           GL  A+ L +  +  Y D+Y +++ V  K    +     L+D V H++Q    S +P LV
Sbjct: 88  GLTEAVGLGIDNISIYSDHYRIFELVMEKSASAEENFALLMDNVQHIRQ-RLTSSFPVLV 147

Query: 254 ARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEE-CDFNRMFAVDGCSHRYC 313
            R+ IKF ++LA E IV +I+  +    K     TC IC ++  +   MF+V  C H +C
Sbjct: 148 TRNQIKFVYELAMETIVSEISIHIPDHDK-----TCSICSDDNFEPELMFSVALCGHEFC 207

Query: 314 SSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQRIKEASIPVSEK 373
             C+K+H+EV+LL G VP+C H  C+  L + +CA  LT K       RI+E SIPV E+
Sbjct: 208 VECVKRHIEVRLLAGGVPRCLHYQCESKLTLANCANLLTSKLKAMWELRIEEESIPVEER 267

Query: 374 VYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCKVPWHNRVSCNDY 433
           VYCP PRCS+LM+  ++   TR+  +      MR C+KC   FC+NCK+PWH+ +SCNDY
Sbjct: 268 VYCPNPRCSSLMSVTKLSNSTREDVT------MRSCVKCGEPFCINCKLPWHSNLSCNDY 327

Query: 434 KRSNNLPT-DDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWKD 493
           K     PT DD+KL  LA+  +WRQC  C ++IEL+EGC H+TCRCGH+FCYKCG +W  
Sbjct: 328 KSLGPNPTADDIKLKALANQKMWRQCENCKNVIELSEGCMHITCRCGHQFCYKCGAKWIT 377

Query: 494 KKATCS 498
            +  C+
Sbjct: 388 GRVFCT 377

BLAST of CmoCh20G005030 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 2.0e-79
Identity = 177/431 (41.07%), Postives = 250/431 (58.00%), Query Frame = 0

Query: 116 QNHFFGEGCSKGVENQGVFKLYFKGLVSEEGIGIERRLV-AGIGVAICNPEGELVFEVKK 175
           Q+ F  + C   V    V+KLYFKGLVS E    ++ +V  G GVAIC+    L + + K
Sbjct: 22  QSTFETQSCVDNV----VYKLYFKGLVSGETATDKKAIVKVGFGVAICDEADNLFYGMNK 81

Query: 176 HLVGNERSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALL 235
            L     ++  A+  ALITGL  ++   +K +   CD   +YQ + G+  P Q+K V LL
Sbjct: 82  SLYEAVINREEADILALITGLYESIHRGVKNVVICCDDRQIYQTIIGREKP-QQKNVHLL 141

Query: 236 DRVTHLQQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLE 295
           + V  L +    S    LVA  D  FA +LA +A+       V    +K L  TC IC +
Sbjct: 142 EEVQRL-RGRLASTGTVLVATRDDNFALRLAIDAL-------VKATQEKPL--TCSICSD 201

Query: 296 ECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKD 355
           + D   M   D C HR+C SC+KQ V+VKL  G+VP C  DGCK +L ++SC+  LTPK 
Sbjct: 202 KTDAEHMLLNDKCLHRHCFSCVKQQVKVKLRSGIVPPCLEDGCKSELTLESCSMVLTPKL 261

Query: 356 MVTMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGL 415
           +   +++++E  IP +EK+YCPY  CS LM+K E+   +R+    A QS +R C+KC  L
Sbjct: 262 IEMWKRKMEEDLIPDAEKIYCPYRSCSMLMSKTEL---SRE----AEQSNVRACIKCSEL 321

Query: 416 FCVNCKVPWHNRVSCNDYKR-SNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHM 475
           FC++CKVPWH+ +SC DYKR  +    +D+ L  LA+  +WRQC +C HMIEL EGC H+
Sbjct: 322 FCIDCKVPWHSDLSCADYKRIHSERLVNDMMLKVLANDQMWRQCSECKHMIELTEGCNHI 381

Query: 476 TCRCGHEFCYKCGGEWKDKKATCSCPLW-----AENHILYNDD--NREFNHDGRFYEEED 535
           TCRCG+EFCY+CG +W  K    SC L       E++ L+ D   N + + D  +  +ED
Sbjct: 382 TCRCGYEFCYRCGHKW-TKYHHYSCQLMDNREDEEDYNLHVDAEVNNDDDDDEDYVFDED 429

Query: 536 EEEELYDSDDE 538
            EE+  D DD+
Sbjct: 442 YEEDDDDDDDD 429

BLAST of CmoCh20G005030 vs. TAIR 10
Match: AT2G25370.2 (RING/U-box superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.1e-77
Identity = 164/379 (43.27%), Postives = 224/379 (59.10%), Query Frame = 0

Query: 121 GEGCSKGVE--NQGVFKLYFKGLVSEEGIG--IERRLVAGIGVAICNPEGELVFEVKKHL 180
           GEG S   +  +  V++L FKGLV+ E +   +E+ + AG GVAIC+ +  L+ E+K+ L
Sbjct: 109 GEGSSPAAKFMDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIKESL 168

Query: 181 VGNERSKIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDR 240
              E S+   E  ALI G                          GK    ++KI  L++ 
Sbjct: 169 RDIEISRRGVEIMALIIG-------------------------RGK---SKKKIDHLVEE 228

Query: 241 VTHL--QQAHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLE 300
           V  +  + A  D+    LVAR+D+KFAF+LAREAI       VD   ++   ETC I  E
Sbjct: 229 VQGILEKMACIDAV---LVARNDVKFAFRLAREAIG---RNSVDVNAEQ--GETCGIFFE 288

Query: 301 ECDFNRMFAVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKD 360
           E D   MF  + C HR+C  C+KQHV+VKL  G  P C   GCKF L ++ C+K LT K 
Sbjct: 289 ETDVEHMFVTEKCLHRHCFPCVKQHVKVKLRSGTEPTCLEYGCKFKLTLERCSKVLTLKL 348

Query: 361 MVTMRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGL 420
           +   +Q++KE SIP +E++YCPYP CS LM+K E       + S A+ S +R C+KC GL
Sbjct: 349 IEMWKQKMKEDSIPAAERIYCPYPNCSMLMSKTE-------LSSEADLSNVRTCVKCCGL 408

Query: 421 FCVNCKVPWHNRVSCNDYKRSNNLP-TDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHM 480
           FC++CKVP H  +S +DYK+ +  P  DD+KL  LA+  +WRQCVKC HMIEL+ GC HM
Sbjct: 409 FCIDCKVPSHTDLSYDDYKKLHPDPLVDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHM 444

Query: 481 TCRCGHEFCYKCGGEWKDK 493
           TCRCG+EFCY+CG EW+ +
Sbjct: 469 TCRCGYEFCYECGIEWQKR 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6T4862.1e-2027.17Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G... [more]
Q8TC416.9e-1628.23Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE... [more]
A2VEA31.5e-1524.75E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1[more]
Q9Y4X51.5e-1524.75E3 ubiquitin-protein ligase ARIH1 OS=Homo sapiens OX=9606 GN=ARIH1 PE=1 SV=2[more]
Q9Z1K51.5e-1524.75E3 ubiquitin-protein ligase ARIH1 OS=Mus musculus OX=10090 GN=Arih1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1FUK70.0e+00100.00uncharacterized protein LOC111448273 OS=Cucurbita moschata OX=3662 GN=LOC1114482... [more]
A0A6J1J8H40.0e+0096.99uncharacterized protein LOC111483577 OS=Cucurbita maxima OX=3661 GN=LOC111483577... [more]
A0A1S3CK991.8e-24877.63uncharacterized protein LOC103501921 OS=Cucumis melo OX=3656 GN=LOC103501921 PE=... [more]
A0A0A0KEP01.1e-24776.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290800 PE=3 SV=1[more]
A0A6J1CGV84.1e-24577.61uncharacterized protein LOC111011310 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
Match NameE-valueIdentityDescription
XP_022943524.10.0e+00100.00uncharacterized protein LOC111448273 [Cucurbita moschata][more]
XP_023511953.10.0e+0096.53uncharacterized protein LOC111776808 [Cucurbita pepo subsp. pepo][more]
KAG6570763.10.0e+0097.23putative E3 ubiquitin-protein ligase rbrA, partial [Cucurbita argyrosperma subsp... [more]
KAG7010609.10.0e+0094.95putative E3 ubiquitin-protein ligase rbrA [Cucurbita argyrosperma subsp. argyros... [more]
XP_022985536.10.0e+0096.99uncharacterized protein LOC111483577 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G60250.12.3e-12343.26zinc finger (C3HC4-type RING finger) family protein [more]
AT2G25370.12.2e-8645.12RING/U-box superfamily protein [more]
AT3G45580.12.5e-8542.35RING/U-box protein with C6HC-type zinc finger [more]
AT5G37560.12.0e-7941.07RING/U-box superfamily protein [more]
AT2G25370.21.1e-7743.27RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 356..429
e-value: 1.3E-13
score: 61.3
coord: 436..498
e-value: 0.01
score: 23.9
IPR002867IBR domainPFAMPF01485IBRcoord: 448..496
e-value: 2.2E-6
score: 27.8
coord: 358..429
e-value: 4.4E-13
score: 49.2
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 284..367
e-value: 1.0E-13
score: 53.0
NoneNo IPR availableGENE3D1.20.120.1750coord: 426..538
e-value: 2.7E-17
score: 65.5
NoneNo IPR availablePANTHERPTHR11685:SF252RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 17..506
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 287..365
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 441..505
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 134..272
e-value: 2.4E-13
score: 52.2
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 152..268
e-value: 2.2E-23
score: 82.4
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 17..506
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 307..316
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 457..500
score: 8.933805
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 289..335
score: 9.509172
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 285..502
score: 25.080683
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 154..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G005030.1CmoCh20G005030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity