CmoCh20G004400 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G004400
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionProtein kinase APK1A
LocationCmo_Chr20: 2053372 .. 2057025 (-)
RNA-Seq ExpressionCmoCh20G004400
SyntenyCmoCh20G004400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGGGGCTCTTGAGCCGCCGAGAAAGAGCGGTCTCTTTAATTCTGGATTCCTCCGCTCCAGCTCCATTGAAATGGGAGGATTTTGGGCGCTTGATTGGGACGCCCGCGTGGACCATGGGATTGAAAACAAGCTCCCATGATCGAGGTATTTCTTCGAGTTCAATCCAATGATAGAAAAGTCAAAAGAGTAACGCCTATCCAATCCCTTACCCATCCAATTTTCTTTCATGTTTATCATTCGGTGCCGATTGCCGTCAGCAATCTTGTTTTTCACCATTGTGATTCATTGAAGACGATGAAATCGGACGCCTCTCAGACTGCAGCGGGAGCCTTTCGGGGCCCTATCCTCTTTGTGAAATGACAGAAAAAGGGGACCAATATACTCGCCATTAACACGAAACTTCCTACCCTCCTGTCCGCTGGGGGCTGTCCAACCCTTCTCGATCTCTATATTTTAGCCAAATGAACAGGGAAATTTTAGCAGGAACTTTAGGGGTTTGCTTATTTCTTATCAATTAAATCTTCTGCTGAAGATTGCCCTAAGGAAACAGTGCTTTGGAACGAGGATCTAACTAGTTGGCTTTTCTTACGTGGGTTGCTGATACAATGAAAAGTGGTTAGTTTAGATTGTTCATCTTTGTTCATATGGGTTTGCTGTGAATGTGCTTGATTTCATTGATGGAATATTGATTTGATGCTTGTTATCTTGTTCTTCCCCTTTTTGGTGGTATCAGCATGAATGTTCTGAAGGTGGGAGTGTCTTTGACTTAATTCTGATGGGTATCTGCTTTAGTGCCAAATCTATATCAGAAAACGATGATAATACAGGTACTACAAGGCTCACTTTATTTGCTAATGAAACAGTGTGGTGGAAGCTTTTAGGCTTTCTGGTTGTCATTTCAAAGTTCTTTTAGATTTCTTATTAATTTACTGAACTTGTTGGGAATATTGTCCTTTTATATGGTTCAGTTTGTTGGTATAACGTATAAACTTGTGTCGATATTGATCATACAATGTGCACGAGTTCTTTCTTTTATTCAAGGCTTTAGTATCTTTAATTGTGAGATCTCACATCGGTAGGGGAGGAGAACGAAACACTCTTTATAAGGGTGTGGAAACTTCTCTCTATCAGACACGTTTTAAGGAAGCACAAAAGGGAAAACCCAAAAATGACAATATCTGCTAGCGGTGTGCTTGGGGCTTGAGGCTGTTACATTAATTGAACTAAAGTAGTCTCAACCGAGCATGTGCACTCATGAGGATTTGGAGTCAAGTGAATGGCTATTATTTCATTACAGGGCATGCATTTGTTTGTGCAGGGGTGAAAAATTCGAAAGATGATGATGATAGCAGTAACATAAACGATAATGGTAGTGCAAGTAGCAAGATCTCGTTGGCTTCAGCGCTGCCAGCCCCTCGGAGCGAGGGTGAAATCTCGCAGTCATCCAATTTGAAGAGCTTCTCTTTGGCAGAGCTCAAAGAGGCAACAAGGAACTTCTGTCCTGACAGTGTCCTGGGAGTACCTGCTTCTAGTTCAGTTTTCAAAGGCTGGATTGATGAGCATTCATTTACTGCTACCAAGCCTGGAACCGGATTGGGTATCGCAGTCAAAAGGCTTCACCGAAACAGTTTTCGAGGTCGCGGAGATTGGTTTGTGAGTTCTCTCTTTGAGATTAGCTGCAGGGTTGTCTCATATTTAACCATCTTCTTGGATAGAATAGAACCTGTGCACTAACATGACTTATTTGTTAAACAAATTTGTGATTCTATGCTGCATAGATCCACTCATGTCATGGCTTCTTTTATCAATTAGGCTGAAGTGAACTTCCTTGGCCAACTTGTTCATTCTCACCTTGTGAAATTGATTGGCTATTGCTTGGAAGACGAGCAACAGCTCATCGTGTCGGAACTTATGCCTCGAGGAAGTTTGGATAATCATCTGTTCCTTCGTTAGTTTTCTGTATCCTTTGATGCAACTCATAAGAACATTATGAAATATTAAGTGTTTGATACTGAAGTTGTAATGGCAGGGGGCTCATATTTTCAACCTCTCTCTTGGGCTCTCCGCTTGAAGGTTGCACTGGGTGCTGCGAAAGGGCTGGCCTTCCTCCACAGTGATGAAAGAAAAGTGATCTTCCGTGACTTGAGGACTTCAATCATTTTGCTTGATTCTGTAAGTCATTGGTGAAAATAAAAATGGCTTCATGGGATTGGGATCTAGCTCCGTTAGTTACGTTGAGGATTGTTGGGAGGGAGTTATTGGGAGGGAGTCCTACATTGGCTAATTAAAGGGATGATCGTAGGTTTATAAGTAAGGCATACATCTCCATTGGTACGAGGTCTTTCAGGGAAACCAAAAGTAAAGCCATGAGAGCTTATGCTTAAAATGGACAATATCATACCATTATGGAGGGTCGTGATTTCTAACATGGTATCAGAGTCATGCCCTTAATGCCCTTAACTTAGCTATGGTTATAGAATCCTCAGGTGTCGAACAAAGAAGTTGTGAGCCTCGAAAGTGTAGTCAAAAGTGATTCACGTGTTGAACAAAGGGTGTACTTTGTTCGAGGACTCCTGAGGAGTCGAGTCTGTTCGAGGGCTCCGTAGGCCTTAGAAGAGGCTCTATGGTGTGCTTTGTTCGAGGGGAGGATTGTAGGGTGGGAGTCCCACATTTACTAATTAAGGGGACGATCGCGAGTTTGTGAGTAAGGAATACATCTCCATTGGTATGAGGCCGCTTGGGGAAACCAAAAGTAAAGCCACGAGAGCTTATGCTCAAAGTAGACAATATCATACTATTATGGAGCGTCGTGATTTCTAACAGTTTACTCGTTCGAAAGATCGGCGCCAGGCCATAAACTTCATTTCTTTTATGTTCAGGACTACAATGCCAAACTCTCTGATCTCGGGTTTTCAAAGACACCGGAGAAACAGGCGGTACGAACAATGTCTCTACCAGGATTACTGAGTACCTCATATGCAGCTCCAGAATATATTGCGACAGGTAATATTCAACACAACCCGAAAACGTGCTCGTTGCTATCAAGTATCTTTGATTCTGCATTCTTTCTCCTGATAAATAACATCATTTTTTCATTACTAATCCGAGAAACTTAAGTCTACTTTGTTAAAACTTGTCGAGTCAACGTAGTTCGTCGTAACAGAACAACGTTTACAGTGTCTCACGTCAACCATTTTGTCTTTTCACTTGCTATAGGTCAGGCAACCACATCAAGTGATGTTTATAGTTTCGGAGTCATTCTACTCGAAATGTTATCTGGAAGAAGAGCTATTGACAAGAATCGACCATTTAGAGAACACAATCTCATCGAATGGGCAAGACCCTACCTTGCAAACGAACGGAAGAGTGTTCGAATAATCGACGACCGTCTCGAAGGCCAATATTCATTGGATGCAGCCTATAAGCTATCTAGTTTGACACTGCAATGCCTGTCCATTGACCCCAAGTCTAGACCGAACATGAACGAGGTTGTAATAGAATTAGAACAGCTACAAGATGCCACATCCATTAACAGCAACCGAACTTCGACCCGAAACCGCATGGCTCGTAGGCATAGCGCCGACGATGCCAATACTCTTCGTAGTGCACGTGCCCGTCCTTGGCCACCACCCATTTCCCCTGATTACTCATAA

mRNA sequence

AGGGGGCTCTTGAGCCGCCGAGAAAGAGCGGTCTCTTTAATTCTGGATTCCTCCGCTCCAGCTCCATTGAAATGGGAGGATTTTGGGCGCTTGATTGGGACGCCCGCGTGGACCATGGGATTGAAAACAAGCTCCCATGATCGAGGTATTTCTTCGAGTTCAATCCAATGATAGAAAAGTCAAAAGAGTAACGCCTATCCAATCCCTTACCCATCCAATTTTCTTTCATGTTTATCATTCGGTGCCGATTGCCGTCAGCAATCTTGTTTTTCACCATTGTGATTCATTGAAGACGATGAAATCGGACGCCTCTCAGACTGCAGCGGGAGCCTTTCGGGGCCCTATCCTCTTTGTGAAATGACAGAAAAAGGGGACCAATATACTCGCCATTAACACGAAACTTCCTACCCTCCTGTCCGCTGGGGGCTGTCCAACCCTTCTCGATCTCTATATTTTAGCCAAATGAACAGGGAAATTTTAGCAGGAACTTTAGGGGTTTGCTTATTTCTTATCAATTAAATCTTCTGCTGAAGATTGCCCTAAGGAAACAGTGCTTTGGAACGAGGATCTAACTAGTTGGCTTTTCTTACGTGGGTTGCTGATACAATGAAAAGTGCATGAATGTTCTGAAGGTGGGAGTGTCTTTGACTTAATTCTGATGGGTATCTGCTTTAGTGCCAAATCTATATCAGAAAACGATGATAATACAGGGGTGAAAAATTCGAAAGATGATGATGATAGCAGTAACATAAACGATAATGGTAGTGCAAGTAGCAAGATCTCGTTGGCTTCAGCGCTGCCAGCCCCTCGGAGCGAGGGTGAAATCTCGCAGTCATCCAATTTGAAGAGCTTCTCTTTGGCAGAGCTCAAAGAGGCAACAAGGAACTTCTGTCCTGACAGTGTCCTGGGAGTACCTGCTTCTAGTTCAGTTTTCAAAGGCTGGATTGATGAGCATTCATTTACTGCTACCAAGCCTGGAACCGGATTGGGTATCGCAGTCAAAAGGCTTCACCGAAACAGTTTTCGAGGTCGCGGAGATTGGTTTGCTGAAGTGAACTTCCTTGGCCAACTTGTTCATTCTCACCTTGTGAAATTGATTGGCTATTGCTTGGAAGACGAGCAACAGCTCATCGTGTCGGAACTTATGCCTCGAGGAAGTTTGGATAATCATCTGTTCCTTCGGGGCTCATATTTTCAACCTCTCTCTTGGGCTCTCCGCTTGAAGGTTGCACTGGGTGCTGCGAAAGGGCTGGCCTTCCTCCACAGTGATGAAAGAAAAGTGATCTTCCGTGACTTGAGGACTTCAATCATTTTGCTTGATTCTGACTACAATGCCAAACTCTCTGATCTCGGGTTTTCAAAGACACCGGAGAAACAGGCGGTACGAACAATGTCTCTACCAGGATTACTGAGTACCTCATATGCAGCTCCAGAATATATTGCGACAGGTCAGGCAACCACATCAAGTGATGTTTATAGTTTCGGAGTCATTCTACTCGAAATGTTATCTGGAAGAAGAGCTATTGACAAGAATCGACCATTTAGAGAACACAATCTCATCGAATGGGCAAGACCCTACCTTGCAAACGAACGGAAGAGTGTTCGAATAATCGACGACCGTCTCGAAGGCCAATATTCATTGGATGCAGCCTATAAGCTATCTAGTTTGACACTGCAATGCCTGTCCATTGACCCCAAGTCTAGACCGAACATGAACGAGGTTGTAATAGAATTAGAACAGCTACAAGATGCCACATCCATTAACAGCAACCGAACTTCGACCCGAAACCGCATGGCTCGTAGGCATAGCGCCGACGATGCCAATACTCTTCGTAGTGCACGTGCCCGTCCTTGGCCACCACCCATTTCCCCTGATTACTCATAA

Coding sequence (CDS)

ATGGGTATCTGCTTTAGTGCCAAATCTATATCAGAAAACGATGATAATACAGGGGTGAAAAATTCGAAAGATGATGATGATAGCAGTAACATAAACGATAATGGTAGTGCAAGTAGCAAGATCTCGTTGGCTTCAGCGCTGCCAGCCCCTCGGAGCGAGGGTGAAATCTCGCAGTCATCCAATTTGAAGAGCTTCTCTTTGGCAGAGCTCAAAGAGGCAACAAGGAACTTCTGTCCTGACAGTGTCCTGGGAGTACCTGCTTCTAGTTCAGTTTTCAAAGGCTGGATTGATGAGCATTCATTTACTGCTACCAAGCCTGGAACCGGATTGGGTATCGCAGTCAAAAGGCTTCACCGAAACAGTTTTCGAGGTCGCGGAGATTGGTTTGCTGAAGTGAACTTCCTTGGCCAACTTGTTCATTCTCACCTTGTGAAATTGATTGGCTATTGCTTGGAAGACGAGCAACAGCTCATCGTGTCGGAACTTATGCCTCGAGGAAGTTTGGATAATCATCTGTTCCTTCGGGGCTCATATTTTCAACCTCTCTCTTGGGCTCTCCGCTTGAAGGTTGCACTGGGTGCTGCGAAAGGGCTGGCCTTCCTCCACAGTGATGAAAGAAAAGTGATCTTCCGTGACTTGAGGACTTCAATCATTTTGCTTGATTCTGACTACAATGCCAAACTCTCTGATCTCGGGTTTTCAAAGACACCGGAGAAACAGGCGGTACGAACAATGTCTCTACCAGGATTACTGAGTACCTCATATGCAGCTCCAGAATATATTGCGACAGGTCAGGCAACCACATCAAGTGATGTTTATAGTTTCGGAGTCATTCTACTCGAAATGTTATCTGGAAGAAGAGCTATTGACAAGAATCGACCATTTAGAGAACACAATCTCATCGAATGGGCAAGACCCTACCTTGCAAACGAACGGAAGAGTGTTCGAATAATCGACGACCGTCTCGAAGGCCAATATTCATTGGATGCAGCCTATAAGCTATCTAGTTTGACACTGCAATGCCTGTCCATTGACCCCAAGTCTAGACCGAACATGAACGAGGTTGTAATAGAATTAGAACAGCTACAAGATGCCACATCCATTAACAGCAACCGAACTTCGACCCGAAACCGCATGGCTCGTAGGCATAGCGCCGACGATGCCAATACTCTTCGTAGTGCACGTGCCCGTCCTTGGCCACCACCCATTTCCCCTGATTACTCATAA

Protein sequence

MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQAVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELEQLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS
Homology
BLAST of CmoCh20G004400 vs. ExPASy Swiss-Prot
Match: Q06548 (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 9.6e-119
Identity = 231/398 (58.04%), Postives = 284/398 (71.36%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGIC SA+  +E   ++G     D        D GS  SK S  S  P+PR+EGEI QS 
Sbjct: 1   MGICLSAQVKAE---SSGASTKYD------AKDIGSLGSKASSVSVRPSPRTEGEILQSP 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSFS AELK ATRNF PDSVLG      VFKGWIDE S TA++PGTGL IAVK+L+++
Sbjct: 61  NLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQD 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
            ++G  +W AEVN+LGQ  H HLVKLIGYCLEDE +L+V E MPRGSL+NHLF RG YFQ
Sbjct: 121 GWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSW LRLKVALGAAKGLAFLHS E +VI+RD +TS ILLDS+YNAKLSD G +K     
Sbjct: 181 PLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIG 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
               +S   + +  YAAPEY+ATG  TT SDVYSFGV+LLE+LSGRRA+DKNRP  E NL
Sbjct: 241 DKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           +EWA+PYL N+RK  R+ID+RL+ QYS++ A K+++L+L+CL+ + K RPNM+EVV  LE
Sbjct: 301 VEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLE 360

Query: 361 QLQDA-TSINSNRTSTRNRMARRHSADDANTLRSARAR 398
            +Q    +I  N   T  RM RR  +  +  + +  AR
Sbjct: 361 HIQSLNAAIGGNMDKTDRRMRRRSDSVVSKKVNAGFAR 389

BLAST of CmoCh20G004400 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 409.1 bits (1050), Expect = 6.0e-113
Identity = 224/387 (57.88%), Postives = 277/387 (71.58%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGIC SA+  + +   +    S + +DS      GS SS +S+ +    PR+EGEI QS 
Sbjct: 1   MGICLSAQIKAVSPGASPKYMSSEANDSL-----GSKSSSVSIRT---NPRTEGEILQSP 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSF+ AELK ATRNF PDSVLG     SVFKGWIDE + TA+KPGTG+ IAVK+L+++
Sbjct: 61  NLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQD 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
            ++G  +W AEVN+LGQ  H +LVKLIGYCLEDE +L+V E MPRGSL+NHLF RGSYFQ
Sbjct: 121 GWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSK---TP 240
           PLSW LRLKVALGAAKGLAFLH+ E  VI+RD +TS ILLDS+YNAKLSD G +K   T 
Sbjct: 181 PLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTG 240

Query: 241 EKQAVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFRE 300
           +K  V T  +    +  YAAPEY+ATG  TT SDVYS+GV+LLE+LSGRRA+DKNRP  E
Sbjct: 241 DKSHVSTRIMG---TYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGE 300

Query: 301 HNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVI 360
             L+EWARP LAN+RK  R+ID+RL+ QYS++ A K+++L L+CL+ + K RPNMNEVV 
Sbjct: 301 QKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVS 360

Query: 361 ELEQLQDATSINSNRTSTRNRMARRHS 385
            LE +Q              R  RR S
Sbjct: 361 HLEHIQTLNEAGGRNIDMVQRRMRRRS 376

BLAST of CmoCh20G004400 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.3e-112
Identity = 212/374 (56.68%), Postives = 273/374 (72.99%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MG C+ AK  SE+         +     S      +++S +S AS  P PRSE EI +++
Sbjct: 1   MGNCWGAKISSESP-------CRSASSPSGGTSKYASNSSVSAASVPPTPRSEDEILEAA 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           N+K+F+  EL+ ATRNF PDSVLG     SVFKGWIDE +   TKPGTG+ IAVK+L++ 
Sbjct: 61  NVKAFAFNELRTATRNFRPDSVLGEGGFGSVFKGWIDEKTLAPTKPGTGMVIAVKKLNQE 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
             +G  +W AEVN+LGQL H +LV+L+GYC+EDEQ+L+V E MPRGSL+NHLF R ++FQ
Sbjct: 121 GHQGHREWLAEVNYLGQLSHPYLVRLVGYCVEDEQRLLVYEFMPRGSLENHLFRRSTHFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSK---TP 240
           PLSW LR+K+ALGAAKGLAFLHSD+ KVI+RD +TS +LLD++Y+AKLSD G +K   T 
Sbjct: 181 PLSWNLRMKIALGAAKGLAFLHSDKVKVIYRDFKTSNVLLDANYDAKLSDFGLAKDGPTG 240

Query: 241 EKQAVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFRE 300
           +K  V T  +    +  YAAPEY+ATG  TT SDVYSFGV+LLEMLSGRRA+DKNRP  E
Sbjct: 241 DKSHVSTRVMG---TYGYAAPEYLATGHLTTKSDVYSFGVVLLEMLSGRRALDKNRPTGE 300

Query: 301 HNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVI 360
           HNL+EWARPYL ++R+  RI+D RL GQYSL  A K ++L LQC+S++ K+RPNM +VV 
Sbjct: 301 HNLVEWARPYLMSKRRIFRILDARLGGQYSLAKAQKAATLALQCISVEAKNRPNMEQVVA 360

Query: 361 ELEQLQDATSINSN 372
            LEQLQD+    +N
Sbjct: 361 VLEQLQDSKETGAN 364

BLAST of CmoCh20G004400 vs. ExPASy Swiss-Prot
Match: P43293 (Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 GN=PBL11 PE=1 SV=2)

HSP 1 Score: 387.9 bits (995), Expect = 1.4e-106
Identity = 201/362 (55.52%), Postives = 265/362 (73.20%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MG CFS +  ++   +T +        S  ++ +GS  S  S AS    PR+EGEI Q++
Sbjct: 1   MGGCFSNRIKTDIASSTWL-------SSKFLSRDGSKGS--STASFSYMPRTEGEILQNA 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLK+FSL+ELK ATRNF PDSV+G      VFKGWIDE S   +KPGTG+ IAVKRL++ 
Sbjct: 61  NLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKRLNQE 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
            F+G  +W AE+N+LGQL H +LVKLIGYCLE+E +L+V E M RGSL+NHLF RG+++Q
Sbjct: 121 GFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFYQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSW  R+++ALGAA+GLAFLH+ + +VI+RD + S ILLDS+YNAKLSD G ++     
Sbjct: 181 PLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMG 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
               +S   + +  YAAPEY+ATG  +  SDVYSFGV+LLE+LSGRRAIDKN+P  EHNL
Sbjct: 241 DNSHVSTRVMGTQGYAAPEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKNQPVGEHNL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           ++WARPYL N+R+ +R++D RL+GQYSL  A K++ L L C+SID KSRP MNE+V  +E
Sbjct: 301 VDWARPYLTNKRRLLRVMDPRLQGQYSLTRALKIAVLALDCISIDAKSRPTMNEIVKTME 353

Query: 361 QL 363
           +L
Sbjct: 361 EL 353

BLAST of CmoCh20G004400 vs. ExPASy Swiss-Prot
Match: O48814 (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.8e-101
Identity = 193/364 (53.02%), Postives = 259/364 (71.15%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MG CFS++          VK     +  S+     S SS+ S ++   A ++EGEI  S+
Sbjct: 1   MGSCFSSR----------VKADIFHNGKSSDLYGLSLSSRKSSSTVAAAQKTEGEILSST 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
            +KSF+  ELK ATRNF PDSV+G      VFKGW+DE + T TKPGTGL IAVK+L++ 
Sbjct: 61  PVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPGTGLVIAVKKLNQE 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
            F+G  +W  E+N+LGQL H +LVKLIGYCLEDE +L+V E M +GSL+NHLF RG+YF+
Sbjct: 121 GFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHLFRRGAYFK 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PL W LR+ VAL AAKGLAFLHSD  KVI+RD++ S ILLD+DYNAKLSD G ++     
Sbjct: 181 PLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFGLARDGPMG 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
            +  +S   + +  YAAPEY+++G     SDVYSFGV+LLE+LSG+RA+D NRP +E NL
Sbjct: 241 DLSYVSTRVMGTYGYAAPEYMSSGHLNARSDVYSFGVLLLEILSGKRALDHNRPAKEENL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           ++WARPYL ++RK + I+D+RL+ QY  + A +++S+ +QCLS +PKSRP M++VV  L+
Sbjct: 301 VDWARPYLTSKRKVLLIVDNRLDTQYLPEEAVRMASVAVQCLSFEPKSRPTMDQVVRALQ 354

Query: 361 QLQD 365
           QLQD
Sbjct: 361 QLQD 354

BLAST of CmoCh20G004400 vs. ExPASy TrEMBL
Match: A0A6J1JF97 (probable serine/threonine-protein kinase PBL9 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111484484 PE=4 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 2.8e-222
Identity = 400/408 (98.04%), Postives = 404/408 (99.02%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGICFSAKSISENDDNTGVKNSKDD+DSSNINDNGSASSKISLASALPAPRSEGEISQSS
Sbjct: 1   MGICFSAKSISENDDNTGVKNSKDDNDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA+KRLHRN
Sbjct: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAIKRLHRN 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
           SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ
Sbjct: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ
Sbjct: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
            VR MSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL
Sbjct: 241 GVRAMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRP+MNEVVIELE
Sbjct: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPSMNEVVIELE 360

Query: 361 QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           QLQD+TSINSNRTSTRN MARRHSADDANTLRSARA PWPPPISPDYS
Sbjct: 361 QLQDSTSINSNRTSTRNHMARRHSADDANTLRSARAHPWPPPISPDYS 408

BLAST of CmoCh20G004400 vs. ExPASy TrEMBL
Match: A0A6J1JCH5 (probable serine/threonine-protein kinase PBL9 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484484 PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 3.4e-220
Identity = 400/415 (96.39%), Postives = 404/415 (97.35%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNT-------GVKNSKDDDDSSNINDNGSASSKISLASALPAPRSE 60
           MGICFSAKSISENDDNT       GVKNSKDD+DSSNINDNGSASSKISLASALPAPRSE
Sbjct: 1   MGICFSAKSISENDDNTGHAFVCAGVKNSKDDNDSSNINDNGSASSKISLASALPAPRSE 60

Query: 61  GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA 120
           GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA
Sbjct: 61  GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA 120

Query: 121 VKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF 180
           +KRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF
Sbjct: 121 IKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF 180

Query: 181 LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF 240
           LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF
Sbjct: 181 LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF 240

Query: 241 SKTPEKQAVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNR 300
           SKTPEKQ VR MSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNR
Sbjct: 241 SKTPEKQGVRAMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNR 300

Query: 301 PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMN 360
           PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRP+MN
Sbjct: 301 PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPSMN 360

Query: 361 EVVIELEQLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           EVVIELEQLQD+TSINSNRTSTRN MARRHSADDANTLRSARA PWPPPISPDYS
Sbjct: 361 EVVIELEQLQDSTSINSNRTSTRNHMARRHSADDANTLRSARAHPWPPPISPDYS 415

BLAST of CmoCh20G004400 vs. ExPASy TrEMBL
Match: A0A6J1JHB1 (probable serine/threonine-protein kinase PBL9 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484484 PE=4 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 8.4e-219
Identity = 397/415 (95.66%), Postives = 402/415 (96.87%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNT-------GVKNSKDDDDSSNINDNGSASSKISLASALPAPRSE 60
           MGICFSAKSISENDDNT       GVKNSKDD+DSSNINDNGSASSKISLASALPAPRSE
Sbjct: 1   MGICFSAKSISENDDNTGHAFVCAGVKNSKDDNDSSNINDNGSASSKISLASALPAPRSE 60

Query: 61  GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA 120
           GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA
Sbjct: 61  GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA 120

Query: 121 VKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF 180
           +KRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF
Sbjct: 121 IKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF 180

Query: 181 LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF 240
           LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF
Sbjct: 181 LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF 240

Query: 241 SKTPEKQAVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNR 300
           SKTPEKQ VR MSLPGLLSTSYAAPEY+  GQATTSSDVYSFGVILLEMLSGRRAIDKNR
Sbjct: 241 SKTPEKQGVRAMSLPGLLSTSYAAPEYLTIGQATTSSDVYSFGVILLEMLSGRRAIDKNR 300

Query: 301 PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMN 360
           PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRP+MN
Sbjct: 301 PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPSMN 360

Query: 361 EVVIELEQLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           EVVIELEQLQD+TSINSNRTSTRN MARRHSADDANTLRSARA PWPPPISPDYS
Sbjct: 361 EVVIELEQLQDSTSINSNRTSTRNHMARRHSADDANTLRSARAHPWPPPISPDYS 415

BLAST of CmoCh20G004400 vs. ExPASy TrEMBL
Match: A0A1S3CKT9 (protein kinase APK1A, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103502082 PE=4 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.2e-177
Identity = 330/408 (80.88%), Postives = 356/408 (87.25%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGIC SAK I E+DDNTGVK SKDD DS+N  DNGSASSK SLAS LPAPRSEGEISQSS
Sbjct: 1   MGICLSAKPICESDDNTGVKYSKDDSDSNNGKDNGSASSKFSLASVLPAPRSEGEISQSS 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSF+  ELKEATRNFCPDSVLG P S SVFKGWIDEHSFTATKPGTG+ IAVKRL+++
Sbjct: 61  NLKSFAYEELKEATRNFCPDSVLGEPGSGSVFKGWIDEHSFTATKPGTGMSIAVKRLNQD 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
           SF+  GDWFAEVNFLGQLVHSHLVKLIGYC++DEQ+L+VSE MPRGSL+NHLFLRGSYFQ
Sbjct: 121 SFQDHGDWFAEVNFLGQLVHSHLVKLIGYCMDDEQRLLVSEFMPRGSLENHLFLRGSYFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSW LRLKVALGAAKGLAFLHSDERKVI+RDLRTS ILLDSDYNAKLSDLGFSK     
Sbjct: 181 PLSWGLRLKVALGAAKGLAFLHSDERKVIYRDLRTSNILLDSDYNAKLSDLGFSKDTGAT 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
             ++       ST YAAPEY+A GQATTSSDVYSFGVILLEMLSGRRA+DKNRPFREHNL
Sbjct: 241 GGKSNVSTRTPSTLYAAPEYLAAGQATTSSDVYSFGVILLEMLSGRRAVDKNRPFREHNL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           IEWARP LAN+RK+ RIID+RLEGQYSLDAAYKLSSLTLQCLSI+PKSRP+MNEVV ELE
Sbjct: 301 IEWARPQLANKRKTARIIDNRLEGQYSLDAAYKLSSLTLQCLSIEPKSRPSMNEVVTELE 360

Query: 361 QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           QLQD TSIN NR S +NRMARR SADDAN  RSARA      +SPDY+
Sbjct: 361 QLQDPTSININRNSKQNRMARRRSADDANIHRSARA------VSPDYT 402

BLAST of CmoCh20G004400 vs. ExPASy TrEMBL
Match: A0A5D3DKG6 (Protein kinase APK1A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G001100 PE=4 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.2e-177
Identity = 330/408 (80.88%), Postives = 356/408 (87.25%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGIC SAK I E+DDNTGVK SKDD DS+N  DNGSASSK SLAS LPAPRSEGEISQSS
Sbjct: 24  MGICLSAKPICESDDNTGVKYSKDDSDSNNGKDNGSASSKFSLASVLPAPRSEGEISQSS 83

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSF+  ELKEATRNFCPDSVLG P S SVFKGWIDEHSFTATKPGTG+ IAVKRL+++
Sbjct: 84  NLKSFAYEELKEATRNFCPDSVLGEPGSGSVFKGWIDEHSFTATKPGTGMSIAVKRLNQD 143

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
           SF+  GDWFAEVNFLGQLVHSHLVKLIGYC++DEQ+L+VSE MPRGSL+NHLFLRGSYFQ
Sbjct: 144 SFQDHGDWFAEVNFLGQLVHSHLVKLIGYCMDDEQRLLVSEFMPRGSLENHLFLRGSYFQ 203

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSW LRLKVALGAAKGLAFLHSDERKVI+RDLRTS ILLDSDYNAKLSDLGFSK     
Sbjct: 204 PLSWGLRLKVALGAAKGLAFLHSDERKVIYRDLRTSNILLDSDYNAKLSDLGFSKDTGAT 263

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
             ++       ST YAAPEY+A GQATTSSDVYSFGVILLEMLSGRRA+DKNRPFREHNL
Sbjct: 264 GGKSNVSTRTPSTLYAAPEYLAAGQATTSSDVYSFGVILLEMLSGRRAVDKNRPFREHNL 323

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           IEWARP LAN+RK+ RIID+RLEGQYSLDAAYKLSSLTLQCLSI+PKSRP+MNEVV ELE
Sbjct: 324 IEWARPQLANKRKTARIIDNRLEGQYSLDAAYKLSSLTLQCLSIEPKSRPSMNEVVTELE 383

Query: 361 QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           QLQD TSIN NR S +NRMARR SADDAN  RSARA      +SPDY+
Sbjct: 384 QLQDPTSININRNSKQNRMARRRSADDANIHRSARA------VSPDYT 425

BLAST of CmoCh20G004400 vs. NCBI nr
Match: KAG6570703.1 (putative serine/threonine-protein kinase PBL9, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010549.1 putative serine/threonine-protein kinase PBL9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 789.6 bits (2038), Expect = 1.2e-224
Identity = 406/408 (99.51%), Postives = 407/408 (99.75%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS
Sbjct: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN
Sbjct: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
           SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ
Sbjct: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ
Sbjct: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
           AVR MSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL
Sbjct: 241 AVRAMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRP+MNEVVIELE
Sbjct: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPSMNEVVIELE 360

Query: 361 QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS
Sbjct: 361 QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 408

BLAST of CmoCh20G004400 vs. NCBI nr
Match: XP_023511977.1 (probable serine/threonine-protein kinase PBL9 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 782.3 bits (2019), Expect = 2.0e-222
Identity = 401/408 (98.28%), Postives = 404/408 (99.02%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGICFSAKSISENDDNTGVKNSKDD+DSSNINDNGSASSKISLASALP PRSEGEISQSS
Sbjct: 1   MGICFSAKSISENDDNTGVKNSKDDNDSSNINDNGSASSKISLASALPVPRSEGEISQSS 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSFSLAELKEATRNFCPDSVLGVPAS SVFKGWIDEHSFTATKPGTGLGIAVKRLHRN
Sbjct: 61  NLKSFSLAELKEATRNFCPDSVLGVPASGSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
           SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ
Sbjct: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ
Sbjct: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
           AVR MSLPGLLSTSYAAPEY+ TGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL
Sbjct: 241 AVRAMSLPGLLSTSYAAPEYLTTGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRP+MNEVVIELE
Sbjct: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPSMNEVVIELE 360

Query: 361 QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS
Sbjct: 361 QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 408

BLAST of CmoCh20G004400 vs. NCBI nr
Match: XP_022986877.1 (probable serine/threonine-protein kinase PBL9 isoform X3 [Cucurbita maxima] >XP_022986880.1 probable serine/threonine-protein kinase PBL9 isoform X3 [Cucurbita maxima])

HSP 1 Score: 780.8 bits (2015), Expect = 5.8e-222
Identity = 400/408 (98.04%), Postives = 404/408 (99.02%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGICFSAKSISENDDNTGVKNSKDD+DSSNINDNGSASSKISLASALPAPRSEGEISQSS
Sbjct: 1   MGICFSAKSISENDDNTGVKNSKDDNDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA+KRLHRN
Sbjct: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAIKRLHRN 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
           SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ
Sbjct: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ
Sbjct: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
            VR MSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL
Sbjct: 241 GVRAMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRP+MNEVVIELE
Sbjct: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPSMNEVVIELE 360

Query: 361 QLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           QLQD+TSINSNRTSTRN MARRHSADDANTLRSARA PWPPPISPDYS
Sbjct: 361 QLQDSTSINSNRTSTRNHMARRHSADDANTLRSARAHPWPPPISPDYS 408

BLAST of CmoCh20G004400 vs. NCBI nr
Match: XP_023511976.1 (probable serine/threonine-protein kinase PBL9 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 775.4 bits (2001), Expect = 2.4e-220
Identity = 401/415 (96.63%), Postives = 404/415 (97.35%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNT-------GVKNSKDDDDSSNINDNGSASSKISLASALPAPRSE 60
           MGICFSAKSISENDDNT       GVKNSKDD+DSSNINDNGSASSKISLASALP PRSE
Sbjct: 1   MGICFSAKSISENDDNTGHAFVCAGVKNSKDDNDSSNINDNGSASSKISLASALPVPRSE 60

Query: 61  GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA 120
           GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPAS SVFKGWIDEHSFTATKPGTGLGIA
Sbjct: 61  GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASGSVFKGWIDEHSFTATKPGTGLGIA 120

Query: 121 VKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF 180
           VKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF
Sbjct: 121 VKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF 180

Query: 181 LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF 240
           LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF
Sbjct: 181 LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF 240

Query: 241 SKTPEKQAVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNR 300
           SKTPEKQAVR MSLPGLLSTSYAAPEY+ TGQATTSSDVYSFGVILLEMLSGRRAIDKNR
Sbjct: 241 SKTPEKQAVRAMSLPGLLSTSYAAPEYLTTGQATTSSDVYSFGVILLEMLSGRRAIDKNR 300

Query: 301 PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMN 360
           PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRP+MN
Sbjct: 301 PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPSMN 360

Query: 361 EVVIELEQLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           EVVIELEQLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS
Sbjct: 361 EVVIELEQLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 415

BLAST of CmoCh20G004400 vs. NCBI nr
Match: XP_022986875.1 (probable serine/threonine-protein kinase PBL9 isoform X2 [Cucurbita maxima] >XP_022986876.1 probable serine/threonine-protein kinase PBL9 isoform X2 [Cucurbita maxima])

HSP 1 Score: 773.9 bits (1997), Expect = 7.1e-220
Identity = 400/415 (96.39%), Postives = 404/415 (97.35%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNT-------GVKNSKDDDDSSNINDNGSASSKISLASALPAPRSE 60
           MGICFSAKSISENDDNT       GVKNSKDD+DSSNINDNGSASSKISLASALPAPRSE
Sbjct: 1   MGICFSAKSISENDDNTGHAFVCAGVKNSKDDNDSSNINDNGSASSKISLASALPAPRSE 60

Query: 61  GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA 120
           GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA
Sbjct: 61  GEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIA 120

Query: 121 VKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF 180
           +KRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF
Sbjct: 121 IKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLF 180

Query: 181 LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF 240
           LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF
Sbjct: 181 LRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGF 240

Query: 241 SKTPEKQAVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNR 300
           SKTPEKQ VR MSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNR
Sbjct: 241 SKTPEKQGVRAMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNR 300

Query: 301 PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMN 360
           PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRP+MN
Sbjct: 301 PFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPSMN 360

Query: 361 EVVIELEQLQDATSINSNRTSTRNRMARRHSADDANTLRSARARPWPPPISPDYS 409
           EVVIELEQLQD+TSINSNRTSTRN MARRHSADDANTLRSARA PWPPPISPDYS
Sbjct: 361 EVVIELEQLQDSTSINSNRTSTRNHMARRHSADDANTLRSARAHPWPPPISPDYS 415

BLAST of CmoCh20G004400 vs. TAIR 10
Match: AT1G07570.2 (Protein kinase superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 6.8e-120
Identity = 231/398 (58.04%), Postives = 284/398 (71.36%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGIC SA+  +E   ++G     D        D GS  SK S  S  P+PR+EGEI QS 
Sbjct: 1   MGICLSAQVKAE---SSGASTKYD------AKDIGSLGSKASSVSVRPSPRTEGEILQSP 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSFS AELK ATRNF PDSVLG      VFKGWIDE S TA++PGTGL IAVK+L+++
Sbjct: 61  NLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQD 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
            ++G  +W AEVN+LGQ  H HLVKLIGYCLEDE +L+V E MPRGSL+NHLF RG YFQ
Sbjct: 121 GWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSW LRLKVALGAAKGLAFLHS E +VI+RD +TS ILLDS+YNAKLSD G +K     
Sbjct: 181 PLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIG 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
               +S   + +  YAAPEY+ATG  TT SDVYSFGV+LLE+LSGRRA+DKNRP  E NL
Sbjct: 241 DKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           +EWA+PYL N+RK  R+ID+RL+ QYS++ A K+++L+L+CL+ + K RPNM+EVV  LE
Sbjct: 301 VEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLE 360

Query: 361 QLQDA-TSINSNRTSTRNRMARRHSADDANTLRSARAR 398
            +Q    +I  N   T  RM RR  +  +  + +  AR
Sbjct: 361 HIQSLNAAIGGNMDKTDRRMRRRSDSVVSKKVNAGFAR 389

BLAST of CmoCh20G004400 vs. TAIR 10
Match: AT1G07570.1 (Protein kinase superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 6.8e-120
Identity = 231/398 (58.04%), Postives = 284/398 (71.36%), Query Frame = 0

Query: 1   MGICFSAKSISENDDNTGVKNSKDDDDSSNINDNGSASSKISLASALPAPRSEGEISQSS 60
           MGIC SA+  +E   ++G     D        D GS  SK S  S  P+PR+EGEI QS 
Sbjct: 1   MGICLSAQVKAE---SSGASTKYD------AKDIGSLGSKASSVSVRPSPRTEGEILQSP 60

Query: 61  NLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRN 120
           NLKSFS AELK ATRNF PDSVLG      VFKGWIDE S TA++PGTGL IAVK+L+++
Sbjct: 61  NLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQD 120

Query: 121 SFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQ 180
            ++G  +W AEVN+LGQ  H HLVKLIGYCLEDE +L+V E MPRGSL+NHLF RG YFQ
Sbjct: 121 GWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQ 180

Query: 181 PLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSKTPEKQ 240
           PLSW LRLKVALGAAKGLAFLHS E +VI+RD +TS ILLDS+YNAKLSD G +K     
Sbjct: 181 PLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIG 240

Query: 241 AVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNL 300
               +S   + +  YAAPEY+ATG  TT SDVYSFGV+LLE+LSGRRA+DKNRP  E NL
Sbjct: 241 DKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNL 300

Query: 301 IEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELE 360
           +EWA+PYL N+RK  R+ID+RL+ QYS++ A K+++L+L+CL+ + K RPNM+EVV  LE
Sbjct: 301 VEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLE 360

Query: 361 QLQDA-TSINSNRTSTRNRMARRHSADDANTLRSARAR 398
            +Q    +I  N   T  RM RR  +  +  + +  AR
Sbjct: 361 HIQSLNAAIGGNMDKTDRRMRRRSDSVVSKKVNAGFAR 389

BLAST of CmoCh20G004400 vs. TAIR 10
Match: AT1G07570.3 (Protein kinase superfamily protein )

HSP 1 Score: 424.9 bits (1091), Expect = 7.5e-119
Identity = 222/366 (60.66%), Postives = 271/366 (74.04%), Query Frame = 0

Query: 33  DNGSASSKISLASALPAPRSEGEISQSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVF 92
           D GS  SK S  S  P+PR+EGEI QS NLKSFS AELK ATRNF PDSVLG      VF
Sbjct: 38  DIGSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVF 97

Query: 93  KGWIDEHSFTATKPGTGLGIAVKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLE 152
           KGWIDE S TA++PGTGL IAVK+L+++ ++G  +W AEVN+LGQ  H HLVKLIGYCLE
Sbjct: 98  KGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLE 157

Query: 153 DEQQLIVSELMPRGSLDNHLFLRGSYFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRD 212
           DE +L+V E MPRGSL+NHLF RG YFQPLSW LRLKVALGAAKGLAFLHS E +VI+RD
Sbjct: 158 DEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRD 217

Query: 213 LRTSIILLDSDYNAKLSDLGFSKTPEKQAVRTMSLPGLLSTSYAAPEYIATGQATTSSDV 272
            +TS ILLDS+YNAKLSD G +K         +S   + +  YAAPEY+ATG  TT SDV
Sbjct: 218 FKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDV 277

Query: 273 YSFGVILLEMLSGRRAIDKNRPFREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAY 332
           YSFGV+LLE+LSGRRA+DKNRP  E NL+EWA+PYL N+RK  R+ID+RL+ QYS++ A 
Sbjct: 278 YSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEAC 337

Query: 333 KLSSLTLQCLSIDPKSRPNMNEVVIELEQLQDA-TSINSNRTSTRNRMARRHSADDANTL 392
           K+++L+L+CL+ + K RPNM+EVV  LE +Q    +I  N   T  RM RR  +  +  +
Sbjct: 338 KVATLSLRCLTTEIKLRPNMSEVVSHLEHIQSLNAAIGGNMDKTDRRMRRRSDSVVSKKV 397

Query: 393 RSARAR 398
            +  AR
Sbjct: 398 NAGFAR 403

BLAST of CmoCh20G004400 vs. TAIR 10
Match: AT2G28930.3 (protein kinase 1B )

HSP 1 Score: 412.5 bits (1059), Expect = 3.9e-115
Identity = 228/390 (58.46%), Postives = 278/390 (71.28%), Query Frame = 0

Query: 1   MGICFSA--KSISENDDNTGVKNSKDDDDSSNINDN-GSASSKISLASALPAPRSEGEIS 60
           MGIC SA  K++S        K       SS  ND+ GS SS +S+ +    PR+EGEI 
Sbjct: 1   MGICLSAQIKAVSPGKPGASPKYM-----SSEANDSLGSKSSSVSIRT---NPRTEGEIL 60

Query: 61  QSSNLKSFSLAELKEATRNFCPDSVLGVPASSSVFKGWIDEHSFTATKPGTGLGIAVKRL 120
           QS NLKSF+ AELK ATRNF PDSVLG     SVFKGWIDE + TA+KPGTG+ IAVK+L
Sbjct: 61  QSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKL 120

Query: 121 HRNSFRGRGDWFAEVNFLGQLVHSHLVKLIGYCLEDEQQLIVSELMPRGSLDNHLFLRGS 180
           +++ ++G  +W AEVN+LGQ  H +LVKLIGYCLEDE +L+V E MPRGSL+NHLF RGS
Sbjct: 121 NQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGS 180

Query: 181 YFQPLSWALRLKVALGAAKGLAFLHSDERKVIFRDLRTSIILLDSDYNAKLSDLGFSK-- 240
           YFQPLSW LRLKVALGAAKGLAFLH+ E  VI+RD +TS ILLDS+YNAKLSD G +K  
Sbjct: 181 YFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDG 240

Query: 241 -TPEKQAVRTMSLPGLLSTSYAAPEYIATGQATTSSDVYSFGVILLEMLSGRRAIDKNRP 300
            T +K  V T  +    +  YAAPEY+ATG  TT SDVYS+GV+LLE+LSGRRA+DKNRP
Sbjct: 241 PTGDKSHVSTRIMG---TYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRP 300

Query: 301 FREHNLIEWARPYLANERKSVRIIDDRLEGQYSLDAAYKLSSLTLQCLSIDPKSRPNMNE 360
             E  L+EWARP LAN+RK  R+ID+RL+ QYS++ A K+++L L+CL+ + K RPNMNE
Sbjct: 301 PGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNE 360

Query: 361 VVIELEQLQDATSINSNRTSTRNRMARRHS 385
           VV  LE +Q              R  RR S
Sbjct: 361 VVSHLEHIQTLNEAGGRNIDMVQRRMRRRS 379

BLAST of CmoCh20G004400 vs. TAIR 10
Match: AT2G28930.1 (protein kinase 1B )

HSP 1 Score: 410.6 bits (1054), Expect = 1.5e-114
Identity = 219/361 (60.66%), Postives = 267/361 (73.96%), Query Frame = 0

Query: 28  SSNINDN-GSASSKISLASALPAPRSEGEISQSSNLKSFSLAELKEATRNFCPDSVLGVP 87
           SS  ND+ GS SS +S+ +    PR+EGEI QS NLKSF+ AELK ATRNF PDSVLG  
Sbjct: 33  SSEANDSLGSKSSSVSIRT---NPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEG 92

Query: 88  ASSSVFKGWIDEHSFTATKPGTGLGIAVKRLHRNSFRGRGDWFAEVNFLGQLVHSHLVKL 147
              SVFKGWIDE + TA+KPGTG+ IAVK+L+++ ++G  +W AEVN+LGQ  H +LVKL
Sbjct: 93  GFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKL 152

Query: 148 IGYCLEDEQQLIVSELMPRGSLDNHLFLRGSYFQPLSWALRLKVALGAAKGLAFLHSDER 207
           IGYCLEDE +L+V E MPRGSL+NHLF RGSYFQPLSW LRLKVALGAAKGLAFLH+ E 
Sbjct: 153 IGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAET 212

Query: 208 KVIFRDLRTSIILLDSDYNAKLSDLGFSK---TPEKQAVRTMSLPGLLSTSYAAPEYIAT 267
            VI+RD +TS ILLDS+YNAKLSD G +K   T +K  V T  +    +  YAAPEY+AT
Sbjct: 213 SVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMG---TYGYAAPEYLAT 272

Query: 268 GQATTSSDVYSFGVILLEMLSGRRAIDKNRPFREHNLIEWARPYLANERKSVRIIDDRLE 327
           G  TT SDVYS+GV+LLE+LSGRRA+DKNRP  E  L+EWARP LAN+RK  R+ID+RL+
Sbjct: 273 GHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQ 332

Query: 328 GQYSLDAAYKLSSLTLQCLSIDPKSRPNMNEVVIELEQLQDATSINSNRTSTRNRMARRH 385
            QYS++ A K+++L L+CL+ + K RPNMNEVV  LE +Q              R  RR 
Sbjct: 333 DQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRR 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q065489.6e-11958.04Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN... [more]
P465736.0e-11357.88Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q65XV82.3e-11256.68Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P432931.4e-10655.52Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 G... [more]
O488141.8e-10153.02Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1JF972.8e-22298.04probable serine/threonine-protein kinase PBL9 isoform X3 OS=Cucurbita maxima OX=... [more]
A0A6J1JCH53.4e-22096.39probable serine/threonine-protein kinase PBL9 isoform X2 OS=Cucurbita maxima OX=... [more]
A0A6J1JHB18.4e-21995.66probable serine/threonine-protein kinase PBL9 isoform X1 OS=Cucurbita maxima OX=... [more]
A0A1S3CKT91.2e-17780.88protein kinase APK1A, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103502082... [more]
A0A5D3DKG61.2e-17780.88Protein kinase APK1A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250... [more]
Match NameE-valueIdentityDescription
KAG6570703.11.2e-22499.51putative serine/threonine-protein kinase PBL9, partial [Cucurbita argyrosperma s... [more]
XP_023511977.12.0e-22298.28probable serine/threonine-protein kinase PBL9 isoform X2 [Cucurbita pepo subsp. ... [more]
XP_022986877.15.8e-22298.04probable serine/threonine-protein kinase PBL9 isoform X3 [Cucurbita maxima] >XP_... [more]
XP_023511976.12.4e-22096.63probable serine/threonine-protein kinase PBL9 isoform X1 [Cucurbita pepo subsp. ... [more]
XP_022986875.17.1e-22096.39probable serine/threonine-protein kinase PBL9 isoform X2 [Cucurbita maxima] >XP_... [more]
Match NameE-valueIdentityDescription
AT1G07570.26.8e-12058.04Protein kinase superfamily protein [more]
AT1G07570.16.8e-12058.04Protein kinase superfamily protein [more]
AT1G07570.37.5e-11960.66Protein kinase superfamily protein [more]
AT2G28930.33.9e-11558.46protein kinase 1B [more]
AT2G28930.11.5e-11460.66protein kinase 1B [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 52..162
e-value: 4.3E-24
score: 86.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 163..388
e-value: 5.5E-52
score: 178.1
NoneNo IPR availablePIRSRPIRSR038189-2PIRSR038189-2coord: 36..239
e-value: 5.5E-24
score: 82.7
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 141..286
e-value: 1.0E-4
score: 17.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..408
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..58
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 27..387
NoneNo IPR availablePANTHERPTHR45621:SF181SERINE/THREONINE-PROTEIN KINASE PBL11-RELATEDcoord: 27..387
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 82..358
e-value: 4.2E-38
score: 131.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 77..362
score: 29.983173
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 55..364

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G004400.1CmoCh20G004400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity