CmoCh20G003570 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G003570
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionE1 ubiquitin-activating enzyme
LocationCmo_Chr20: 1751619 .. 1759551 (+)
RNA-Seq ExpressionCmoCh20G003570
SyntenyCmoCh20G003570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTACGCTCCTAATCGGTACGTTTCCTTCTCCCTTTTTCTCTTAATTAATCTCTTTTTCTCTCTCTTTTTTGTTTTCGTTTCGCTGTTTCTTTCCACTCTCAAATCTGTGCCCATTTCCTGGATCATGTGGCCTGAGCTAGGGTTTATCGGTCTTCCTCCGATCGGTGATCTTTTAGTGTTTCGAGAGGTTTATGGCGGAGTTCTTGATGAATTATTTCTGGGTTTTGTTGTATTGCTTGTTTTCTTCATCATGCTGAGGTCTTTTGGGGTTTTCAGCAGCCTACTGCACTTTATGCTTCCTAGGAAGAGAGCTGGTGAAGAAGGTGTGGCTGTAGAAGAAAAAACTGATAACAGCAGCAGCAGTAACAACAACAACAACAACAACAACAGCGTTCGCAACGAGGGTGCGTCTCTGATCAAGAAGCAGCGGATCGATTCCGACTCCAACGCCAACAGTAAGGTAGCTGCTGTTGCCACCGGAGCTAACAACATCGTGTACGACGGTGCATCGTTGATCATGGCCTCGGCTAATTCTAATCCGCCGGATATTGATGAGGATCTGCACAGCCGACAACTTGCTGTGTATGGCCGCGAAACCATGCGGAAGCTTTTTGCGTCCAATGTCCTCATTTCGGGGATGCAAGGTCTTGGCGCTGAGATTGGTAAAAACTGACGGAACTCCTTTTTTTTTTTGCTAATTTTGCTTATACTGATCCGCTCCCTTGCATGTTTAGGGTCTGGTACCTGTGTCTTTTTGTTTTTGGCCGTGCTAGATGTTCATTAATAACTAACGAGCATCTGACGGTCGACCTTTTTGGCTTAAAATGTCCACACCTTCTATATATATGTGTGTATATATATTTACATTGAAGCGCATTCTCTTGGTCTTTCATTAGAGCTGACTATGCTTTCTGTTTTTGCTTAATATTGTTATCTTATTTGGATTTGATTTGCGAGGGGATTTTCTTCATGCTTGCTTACTTTTGGCTCTTAATGTTTAGTTCTTAAGTTTTCTATTTTAACGATGATTTATTGTATTTTGCGTCTCTTCCTGCAGCAAAGAATGTTATTCTTGCTGGGGTGAAGTCTGTGACCTTGCATGACGAAGGTATAGTGGAGCTATGGGATCTGTCTAGTAATTTTGTGTTCTCAGAGAGTGATATTGGCAAAAACAGAGCACTTGCCTCTGCTCAGAAGTTGCAAGATCTCAACAATTCTGTCATTGTACACACCTCGATGACTAAGTTAGTTATGGAACAACTTTCCGAATTTGAGGTATTCTATCTTTATGCATATTTTCTACTCATTTCTTTAAGGAGTGGTTAGATTCATCATATACTACATGTGTTCCTAAAAAAACGCATCCATTGGAATTGGAAGCCTAGCTTATTAAAACCATTTGATACATTGTATGCGAAATAAGAAGTTTAGTGACAAGGCCTACACTTCACTTCCAAATGTTCATATTTCTCTATTATTTTGTCTGGAATTTTGTATAGAACTGTAGAAGTTATGGTCATCTAATTTGAAAATCCCACATGACCTTCTGATGAAGGTCAACTGGTATTCTCCTCACTTTATTTGTTTATAATTGTGAGCTTATGTCTTAGATTCTTGTATCATGTATCATTCTTATGTTACGTAGTAGACTGTTTCTTTATATTAGCCTATTGAGCACAAGATTCATGGCAGATCATCTCCAAGGCTTCCATTTGAAATGGTGATTGATACCTTCTCATACTCCTGAACTGCCTCTTGATGTCAAAGTAAAATTTGGTTTCAGAGTTCCCACCAAATTCTTCAAGGCACAAAAACAATCCAATGAGAGAGCCTTTTGTTTTCTTGTGAATGTTACACCAATTTGGTCAGAATGTGCATTTTGGATGTATATTTTTGCAAGTGTTGAAGCCTCAATTAGAAAACTCTAAACGACCCCCGTTAACTCTTTGAGGGAGTTGCGTTATCTAAGTGTTGTTAGGAAGTCTATTGGCATTTGAAGTTTTCTTCAGAGAGGCCATGGTTGGTAATTTTGCACGTCATATCATTTAGACATACCATGAGACCGTTGCCTGAACCTTTGCTAGTAGTTGAAAGAACACCGTGATTGGTTGCCATAAGGTTTAGTCTCTCTATCTTGTAGCCATTGGAGTTCTTGTGGAAGTAAAAGAAAGGGGGTCCTTACTATGACGTTTCCAATATTTTAGAAATGTGACTATTTTTTTTATTAATATAGACGACTATGGTGGTATGTATGAGCTAGAGTAGTATCCATAAATGGTGGTGGGCAAATAGTGTTGACTTTGTTTGGAGGAGCTTTTTCCTAGATTTTGTTGGGGTCCATAGTTAAGCTTAAACCTTTATTTGGTAAGAGAGTTGGAAAAGTTTCCTAGTAGTAGCATTGGCAATGTCACAACCATACTCTCTCCGAGAGAGTAGGTTGTGACCCTCATGTCAAGACAAGCAAAGCGGCGGCTCTCAAGTGGTGTTCGTTTGGGCACGCAAGGGTCAATATAGAAAAATGTCATGATGTGACTGAGGAGGAGGGTGCATCATCCAACACACCATATAATGTGTGCATTGAAGAGGAAACGAGACATAAGAAAGATAGGGACAATGAGTGGACATGAGTGTCAAAGATAGGCTTGTTGAAGGGTCGGGTAGGGCCTTGAGCCTTAACCTTGAAAATTGGGGTTTTGGAGGGAACTTGGGCATGCGGGTCTTGAGTCAAGTCCATCCATAGGAAATATGAAAACTCATGAAATTTGGTGTCAATCGGACCCTGAACGAACGCTCCACGACACTGTATGTCCATTCACCAAAATCATGTCTACACCTGCCACTTTGGAAATGCCTCAGGATGTGCTATAGGGGGAGGCATGGTGTTACCTCTCCACCACCTCTGGGCCCGGTGACCCTAAGCGAGCTACCCTGTGGCATGTGTGTCACATTGAGGGCAAGGATTTTGAGATGAGTGTCTCGGGCGACTTCCTTTGAAATGGGTCTTTCAAGTACATTTTTTTAGCAAAAACAGGTGTGTTGGTATTGCACCCTTGCCCGAGTATCGAAGGTTGGATTTTGCATCATCGCTACTTGGTACGGTATCCGTAAATGACATGGTATGGACATAGGAGGGCCAATGCCTCCATAAGAACTTGGGTGGATGGATGTTTAGGATGTCCTGAGATTATGATTAACACGAGGGCCTGTTCTTGATGGCATGATCGAGTGAGTTAAAATGGCTGACGACACTCCATAACTTGGGTTGACGTCAAGACATATGGTTGTTGGGAACCAAGATGACAAGATGAGATGTGCATTGAGAAACCGTGGCCTTGAGTAGAGGTAAGTTGGTTGTGTGGTAGATGATTGAACATGCACTTGCAAGCAGTGTGTGGTCGAACAAGCTTGGATTCTGCTTGAGCAATGTTTGGTTGGTGAAGACAAACCTTGCCTAAGGTTTGGTGAAACCACAAAGCCGGGCGAATAATGAATGGGTCTTGAGTTCTCCTTAAGGGTGGTCGTGAGCATGTCAAGATCATGATGTTGCACAGTTAAAATAGTACGACCGTGAAACCTAGCATCTAGATATCAAAGGCCGAATTCCTGCATTTATGGAAACTACCATTTAATTGTAGTACTTGACTTTGAGAACGTGTTGAAGGTGAACGGGGATAGGGGTTTGATTATTTGAGAATTTTGAAATGAGTGATCTTTATTAGATTGCTCAAGGAAGGTTGGTTTGAGACAGATATTGGAGAGGAAAAGAACGTGTCAACAACACGAGATGTTTACAGTGATTTTTTCCTAGTGGTGGTTGGTTAAGATTTTTGACCATCAGGGTCCGCTCTAGACAAAATGACCCGCTCTGAAATGCTCCAGTGTTCTTGAAGGCATTGAAGAGTACCTAGATTGTTCAAAATGTATTCCGTTTGCAAAGCGATTTACTGATTTCCATTTTCTGCTCTTCTGGTGAGGGTGTTAGACATAAGCTCCGAGCATGAGTGCGTTCAGATCTAGACACTTGTGCCTGAAGGTTACATAATATTAATGAAGTCTATGCCAAGTGCTGCTTAAGATTTTACATATATTTAAATATAAACAGTGTCAACTGAGACTGGTTACAGGAAATTGTTCCTGATTGATTATATTGGATTGATAAATTTCAGCCAATTCACAATATTATCTTTCTACCTCTCTACCTCAGCTTCCTTCTCTCAAAATAGAAATTCCATACTAAAAATGTGGATTGGAGCTCATCAAACTACGAGGAAAACGGCTCATAAGCTCTTTCTTATGATCTAAAAATGTTCAAAAAAAGTTTCTTTGGGATAATTTTATTACAGATTTCTCCTGGAATTCTTTGCAAGAGAGAAAAAGGTGTATCTTCCAAGATAAAGTCTCATTTCGTAGTTTTTTCGAGGCTGTAGTTAATGTGGTTGTTTGTGGCTCTTTTCCATACCGATAGCTACTCTACTCTTTAATTTATGAGGCCTTTTGTAATCTTGCCCTTGGAGTTCCCCCCTTTCAATATCATGCTAAGAACGAAACTCTTTCTTATTAAAAAGAAAAAGGAAAGGCGAACTGAAAATTTAAACGAAAAATTTAGTTTTGAGATGATAAAATCACCATAGAATTTTTTTATCAAATTTTCAACTGGTTGCAAATTCATAGAAAGGATCATGGCTTCTGCTAGTTAAAATATATAATAGTTGGAGTTAACATCAATTATGTGTGCTTGTTTATCATGTATATGAGTTTCATGCGCATGATGTAACTTCTCTGTGGATGCAGGCTGTTGTTTTCACTGATACTGGCCTTGACAAGGCCATGGAATTCAATGACTTCTGTCATAACCACCAGCCTCCTATTGCATTTATCAAGACTGAAGTTAGAGGGCTCTTTGGTTCGGTATTTTGTGACTTTGGTCCCGAGTTCACTGTTTATGATGTGTATGGAGAGGACCCACACACTGGCATAATTGCGTCCATTAGCAATGACAATCCTGCACTCGTTTCCTGTGTTGATGATGAGAGGCTTGAGTTTCAGGATGGAGATCTTGTGGTGTTTTCTGAAGTTCATGGTATGACAGAGCTGAATGATGGGAAGCCGAGAAGGATTAAAAATTGCAGGGCCTATTCATTTACTCTCGAGGAGGATACTACAAACTTTGGCAGCTATGAGAAAGGTGGCATTGTCACACAGGTGAAAGAGCCCAAGATGTTGAACTTCAAGCCATTAAGAGAAGCAATCAACGATCCTGGTGACTTCCTTCTCAGTGATTTCTCCAAGTTCGATCGTCCTCCTCTCCTACACTTGGCATTCCTGGCCTTAGATAAATTCGTGACTGAGTTGGGTCGCCTACCAGTTGCTGGTTCTGAGGAGGATGCTCAAAAGCTGATTTCTGTTGCCAGTAATGTTAACGAGAGTCTAGGAGACGGGAGAGTCGAAGATATTAATCCTAAGCTTTTGAGACATTTTGCATTTGGTGCCAAGGCAGTACTGAATCCGATGGCTGCCATGTTTGGTGGTATTGTAGCTCAAGAGGTTCTCAAAGCGTGCTCCGGAAAGTTTCATCCACTTGTCCAGGTTAGTAGATTACTAATGTTTTGAGTCTGATAAATATGCCTTAGCGACCTTCGTGTTGCAACGCCTTGGACCTTTTTGTTCAATGAAGTACATACAAGTTTATCTCTCTCTGCTTATATGAAATAGATCATAATCTATGGGAACAGTGAAGTTATTGCAATAACTCTCCCTGCATGCTGGGATTGTCATTTGGTTACTGTTTCTTGCATCCTTACGGTATTTTCTTTCGAATATGCAGTTCTTCTATTTCGACTCGTTGGAGTCTCTTCCCACAGAGTCATTGGAAGCCAGTGATTTTAGACCCTTGAATAGTCGTTATGATGCACAGATTTCTGTGTTTGGGTCTAAACTTCAGAAGAAACTGGAAAATGCCAAAGTCTTTATGGTTGGATCTGGTGCTCTAGGTTGTGAGTTCTTGAAGAACCTTGCACTTATGGGAGTTTCATGTAGCAACGAAGGGAAGCTAACGATTACGGATGATGATGTAATTGAAAAAAGCAACCTTAGTCGGCAGTTCCTTTTCCGTGACTGGAACATTGGGCAGGCGAAATCTACTGTCGCTGCTTCTGCTGCTGTTGCAATTAATAAGCACCTCAACATCGAAGCTTTGCAGAATCGTGTTAGTCCCGAGACTGAAAACGTGTTCGATGATAGCTTTTGGGAGAATTTGAATGTCGTGGTTAACGCACTAGACAATGTAAATGCAAGGCTCTATGTCGATCAAAGGTGCTTATACTTCCAGAAACCACTTCTAGAATCTGGAACTCTTGGTGCTAAATGCAATACTCAAATGGTCATCCCTCACCTGACTGAAAACTATGGGGCATCAAGAGACCCCCCTGAGAAACAAGCGCCCATGTGCACTGTGCATTCGTTTCCACATAATATCGACCACTGTTTGACATGGGCTCGATCTGAGTTCGAGGGCTTGCTTGAGAAGACTCCTACTGATGTGAACGCTTATCTATCAAATCCCAGTGAATACACTTCTGCAATGATGAATGCTGGTGATGCTCAGTCTAGGGACACTTTAGAGCGCATTCTCGAGTGTCTTGATAGAGAAAGATGCGAGACGTTTGAAGACTGCATCACGTGGGCTCGCTTGAAGTACTGTTCTTCATGTTCTCAACTCTCATCTGTCTCCATTTAACTTTTGATGTTCAGTTGTTTAATGTTATTGATTCCTGTTGTGATATTATAACTTCGACAGGTTTGAAGATTATTTTTCGAACCGTGTGAAGCAGTTGATATACACATTTCCTGAAGATGCTGTAACTAGCAATGGGGCACCATTCTGGTCTGCCCCAAAGAGATTTCCCCATCCACTTCAGTTTTCAACTGCCGATCAAAGCTACCTTCATTTTGTTTTGGCGGCGGCTATACTAAGAGCAGAATCATATGCCATTCCGATTCCTGACTGGGTTAAGAACCCCACGAAATTGGCCGATGCAGTTGACCGAGTTATAGTACCAGATTTTATGCCCAAAAAAGATGCCAAGATTGTGACTGATGAGAAGGCAACCAGTCTCTCTACAGCATCAGTTGACGATGCAGCCGTTATCCACGACCTGGTGAACAAATTGGAGGATACACGCAGGAAGCTACCAGAGGGATTCAGGATGAAACCGATCCAGTTTGAGAAGGTCGTTCGTTAGAGGATTTATTGATATTGTTATGTGTGATCAAAATTATTTCTGGAGATCTTAGAATCAATGGTGTAAATCTAACTACCTTAGTTTGGATGGTGCAGGATGATGATTCGAATTTCCATATGGATCTCATAGCTGGGCTGGCTAACATGAGAGCGAGGAATTACAGCATTCCTGAAGTAGACAAGTTGAAAGCCAAGTTCATTGCTGGAAGGATCATCCCCGCCATTGCTACCTCTACTGCAATGGCTACAGGTCTCGTCTGCCTCGAACTGTACAAAGTTCTAGATGGCGGTCACAAGGTGGAGGATTACCGTAACACGTTTGCGAACCTTGCATTGCCTCTGTTCTCCATGGCCGAGCCAGTCCCGCCCAAGGTCATCAAGCACCGGGACATGAGCTGGACTGTCTGGGACAGATGGATCATCAAAGACAACCCTACACTCAGACAACTTATTGAATGGTTGAAGAACAAGGGATTGAATGCCTACAGCATCTCGTGCGGTAGCTGTCTCCTGTACAATAGTATGTTCCCTCGACACAGAGATCGAATGGACAAGAAGGTAGTTGATTTAGCTCGAGATGTTGCCAAGGTGGAACTGCCTCCATACCGTCGACATTTGGATGTTGTCGTAGCATGCGAGGATGACGAGGATAATGATATTGACATCCCTCTGGTGTCGGTTTACTTCCGTTAG

mRNA sequence

TTACGCTCCTAATCGGTACGTTTCCTTCTCCCTTTTTCTCTTAATTAATCTCTTTTTCTCTCTCTTTTTTGTTTTCGTTTCGCTGTTTCTTTCCACTCTCAAATCTGTGCCCATTTCCTGGATCATGTGGCCTGAGCTAGGGTTTATCGGTCTTCCTCCGATCGGTGATCTTTTAGTGTTTCGAGAGGTTTATGGCGGAGTTCTTGATGAATTATTTCTGGGTTTTGTTGTATTGCTTGTTTTCTTCATCATGCTGAGGTCTTTTGGGGTTTTCAGCAGCCTACTGCACTTTATGCTTCCTAGGAAGAGAGCTGGTGAAGAAGGTGTGGCTGTAGAAGAAAAAACTGATAACAGCAGCAGCAGTAACAACAACAACAACAACAACAACAGCGTTCGCAACGAGGGTGCGTCTCTGATCAAGAAGCAGCGGATCGATTCCGACTCCAACGCCAACAGTAAGGTAGCTGCTGTTGCCACCGGAGCTAACAACATCGTGTACGACGGTGCATCGTTGATCATGGCCTCGGCTAATTCTAATCCGCCGGATATTGATGAGGATCTGCACAGCCGACAACTTGCTGTGTATGGCCGCGAAACCATGCGGAAGCTTTTTGCGTCCAATGTCCTCATTTCGGGGATGCAAGGTCTTGGCGCTGAGATTGTTTTCTATTTTAACGATGATTTATTGTATTTTGCGTCTCTTCCTGCAGCAAAGAATGTTATTCTTGCTGGGGTGAAGTCTGTGACCTTGCATGACGAAGGTATAGTGGAGCTATGGGATCTGTCTAGTAATTTTGTGTTCTCAGAGAGTGATATTGGCAAAAACAGAGCACTTGCCTCTGCTCAGAAGTTGCAAGATCTCAACAATTCTGTCATTGTACACACCTCGATGACTAAGTTAGTTATGGAACAACTTTCCGAATTTGAGCCTATTGAGCACAAGATTCATGGCAGATCATCTCCAAGGCTTCCATTTGAAATGACGACTATGGTGGCTGTTGTTTTCACTGATACTGGCCTTGACAAGGCCATGGAATTCAATGACTTCTGTCATAACCACCAGCCTCCTATTGCATTTATCAAGACTGAAGTTAGAGGGCTCTTTGGTTCGGTATTTTGTGACTTTGGTCCCGAGTTCACTGTTTATGATGTGTATGGAGAGGACCCACACACTGGCATAATTGCGTCCATTAGCAATGACAATCCTGCACTCGTTTCCTGTGTTGATGATGAGAGGCTTGAGTTTCAGGATGGAGATCTTGTGGTGTTTTCTGAAGTTCATGGTATGACAGAGCTGAATGATGGGAAGCCGAGAAGGATTAAAAATTGCAGGGCCTATTCATTTACTCTCGAGGAGGATACTACAAACTTTGGCAGCTATGAGAAAGGTGGCATTGTCACACAGGTGAAAGAGCCCAAGATGTTGAACTTCAAGCCATTAAGAGAAGCAATCAACGATCCTGGTGACTTCCTTCTCAGTGATTTCTCCAAGTTCGATCGTCCTCCTCTCCTACACTTGGCATTCCTGGCCTTAGATAAATTCGTGACTGAGTTGGGTCGCCTACCAGTTGCTGGTTCTGAGGAGGATGCTCAAAAGCTGATTTCTGTTGCCAGTAATGTTAACGAGAGTCTAGGAGACGGGAGAGTCGAAGATATTAATCCTAAGCTTTTGAGACATTTTGCATTTGGTGCCAAGGCAGTACTGAATCCGATGGCTGCCATGTTTGGTGGTATTGTAGCTCAAGAGGTTCTCAAAGCGTGCTCCGGAAAGTTTCATCCACTTGTCCAGTTCTTCTATTTCGACTCGTTGGAGTCTCTTCCCACAGAGTCATTGGAAGCCAGTGATTTTAGACCCTTGAATAGTCGTTATGATGCACAGATTTCTGTGTTTGGGTCTAAACTTCAGAAGAAACTGGAAAATGCCAAAGTCTTTATGGTTGGATCTGGTGCTCTAGGTTGTGAGTTCTTGAAGAACCTTGCACTTATGGGAGTTTCATGTAGCAACGAAGGGAAGCTAACGATTACGGATGATGATGTAATTGAAAAAAGCAACCTTAGTCGGCAGTTCCTTTTCCGTGACTGGAACATTGGGCAGGCGAAATCTACTGTCGCTGCTTCTGCTGCTGTTGCAATTAATAAGCACCTCAACATCGAAGCTTTGCAGAATCGTGTTAGTCCCGAGACTGAAAACGTGTTCGATGATAGCTTTTGGGAGAATTTGAATGTCGTGGTTAACGCACTAGACAATGTAAATGCAAGGCTCTATGTCGATCAAAGGTGCTTATACTTCCAGAAACCACTTCTAGAATCTGGAACTCTTGGTGCTAAATGCAATACTCAAATGGTCATCCCTCACCTGACTGAAAACTATGGGGCATCAAGAGACCCCCCTGAGAAACAAGCGCCCATGTGCACTGTGCATTCGTTTCCACATAATATCGACCACTGTTTGACATGGGCTCGATCTGAGTTCGAGGGCTTGCTTGAGAAGACTCCTACTGATGTGAACGCTTATCTATCAAATCCCAGTGAATACACTTCTGCAATGATGAATGCTGGTGATGCTCAGTCTAGGGACACTTTAGAGCGCATTCTCGAGTGTCTTGATAGAGAAAGATGCGAGACGTTTGAAGACTGCATCACGTGGGCTCGCTTGAAGTTTGAAGATTATTTTTCGAACCGTGTGAAGCAGTTGATATACACATTTCCTGAAGATGCTGTAACTAGCAATGGGGCACCATTCTGGTCTGCCCCAAAGAGATTTCCCCATCCACTTCAGTTTTCAACTGCCGATCAAAGCTACCTTCATTTTGTTTTGGCGGCGGCTATACTAAGAGCAGAATCATATGCCATTCCGATTCCTGACTGGGTTAAGAACCCCACGAAATTGGCCGATGCAGTTGACCGAGTTATAGTACCAGATTTTATGCCCAAAAAAGATGCCAAGATTGTGACTGATGAGAAGGCAACCAGTCTCTCTACAGCATCAGTTGACGATGCAGCCGTTATCCACGACCTGGTGAACAAATTGGAGGATACACGCAGGAAGCTACCAGAGGGATTCAGGATGAAACCGATCCAGTTTGAGAAGGTCGTTCAGGATTTATTGATATTGTTATGTGTGATCAAAATTATTTCTGGAGATCTTAGAATCAATGGTGTAAATCTAACTACCTTAGTTTGGATGGTGCAGGATGATGATTCGAATTTCCATATGGATCTCATAGCTGGGCTGGCTAACATGAGAGCGAGGAATTACAGCATTCCTGAAGTAGACAAGTTGAAAGCCAAGTTCATTGCTGGAAGGATCATCCCCGCCATTGCTACCTCTACTGCAATGGCTACAGGTCTCGTCTGCCTCGAACTGTACAAAGTTCTAGATGGCGGTCACAAGGTGGAGGATTACCGTAACACGTTTGCGAACCTTGCATTGCCTCTGTTCTCCATGGCCGAGCCAGTCCCGCCCAAGGTCATCAAGCACCGGGACATGAGCTGGACTGTCTGGGACAGATGGATCATCAAAGACAACCCTACACTCAGACAACTTATTGAATGGTTGAAGAACAAGGGATTGAATGCCTACAGCATCTCGTGCGGTAGCTGTCTCCTGTACAATAGTATGTTCCCTCGACACAGAGATCGAATGGACAAGAAGGTAGTTGATTTAGCTCGAGATGTTGCCAAGGTGGAACTGCCTCCATACCGTCGACATTTGGATGTTGTCGTAGCATGCGAGGATGACGAGGATAATGATATTGACATCCCTCTGGTGTCGGTTTACTTCCGTTAG

Coding sequence (CDS)

ATGTGGCCTGAGCTAGGGTTTATCGGTCTTCCTCCGATCGGTGATCTTTTAGTGTTTCGAGAGGTTTATGGCGGAGTTCTTGATGAATTATTTCTGGGTTTTGTTGTATTGCTTGTTTTCTTCATCATGCTGAGGTCTTTTGGGGTTTTCAGCAGCCTACTGCACTTTATGCTTCCTAGGAAGAGAGCTGGTGAAGAAGGTGTGGCTGTAGAAGAAAAAACTGATAACAGCAGCAGCAGTAACAACAACAACAACAACAACAACAGCGTTCGCAACGAGGGTGCGTCTCTGATCAAGAAGCAGCGGATCGATTCCGACTCCAACGCCAACAGTAAGGTAGCTGCTGTTGCCACCGGAGCTAACAACATCGTGTACGACGGTGCATCGTTGATCATGGCCTCGGCTAATTCTAATCCGCCGGATATTGATGAGGATCTGCACAGCCGACAACTTGCTGTGTATGGCCGCGAAACCATGCGGAAGCTTTTTGCGTCCAATGTCCTCATTTCGGGGATGCAAGGTCTTGGCGCTGAGATTGTTTTCTATTTTAACGATGATTTATTGTATTTTGCGTCTCTTCCTGCAGCAAAGAATGTTATTCTTGCTGGGGTGAAGTCTGTGACCTTGCATGACGAAGGTATAGTGGAGCTATGGGATCTGTCTAGTAATTTTGTGTTCTCAGAGAGTGATATTGGCAAAAACAGAGCACTTGCCTCTGCTCAGAAGTTGCAAGATCTCAACAATTCTGTCATTGTACACACCTCGATGACTAAGTTAGTTATGGAACAACTTTCCGAATTTGAGCCTATTGAGCACAAGATTCATGGCAGATCATCTCCAAGGCTTCCATTTGAAATGACGACTATGGTGGCTGTTGTTTTCACTGATACTGGCCTTGACAAGGCCATGGAATTCAATGACTTCTGTCATAACCACCAGCCTCCTATTGCATTTATCAAGACTGAAGTTAGAGGGCTCTTTGGTTCGGTATTTTGTGACTTTGGTCCCGAGTTCACTGTTTATGATGTGTATGGAGAGGACCCACACACTGGCATAATTGCGTCCATTAGCAATGACAATCCTGCACTCGTTTCCTGTGTTGATGATGAGAGGCTTGAGTTTCAGGATGGAGATCTTGTGGTGTTTTCTGAAGTTCATGGTATGACAGAGCTGAATGATGGGAAGCCGAGAAGGATTAAAAATTGCAGGGCCTATTCATTTACTCTCGAGGAGGATACTACAAACTTTGGCAGCTATGAGAAAGGTGGCATTGTCACACAGGTGAAAGAGCCCAAGATGTTGAACTTCAAGCCATTAAGAGAAGCAATCAACGATCCTGGTGACTTCCTTCTCAGTGATTTCTCCAAGTTCGATCGTCCTCCTCTCCTACACTTGGCATTCCTGGCCTTAGATAAATTCGTGACTGAGTTGGGTCGCCTACCAGTTGCTGGTTCTGAGGAGGATGCTCAAAAGCTGATTTCTGTTGCCAGTAATGTTAACGAGAGTCTAGGAGACGGGAGAGTCGAAGATATTAATCCTAAGCTTTTGAGACATTTTGCATTTGGTGCCAAGGCAGTACTGAATCCGATGGCTGCCATGTTTGGTGGTATTGTAGCTCAAGAGGTTCTCAAAGCGTGCTCCGGAAAGTTTCATCCACTTGTCCAGTTCTTCTATTTCGACTCGTTGGAGTCTCTTCCCACAGAGTCATTGGAAGCCAGTGATTTTAGACCCTTGAATAGTCGTTATGATGCACAGATTTCTGTGTTTGGGTCTAAACTTCAGAAGAAACTGGAAAATGCCAAAGTCTTTATGGTTGGATCTGGTGCTCTAGGTTGTGAGTTCTTGAAGAACCTTGCACTTATGGGAGTTTCATGTAGCAACGAAGGGAAGCTAACGATTACGGATGATGATGTAATTGAAAAAAGCAACCTTAGTCGGCAGTTCCTTTTCCGTGACTGGAACATTGGGCAGGCGAAATCTACTGTCGCTGCTTCTGCTGCTGTTGCAATTAATAAGCACCTCAACATCGAAGCTTTGCAGAATCGTGTTAGTCCCGAGACTGAAAACGTGTTCGATGATAGCTTTTGGGAGAATTTGAATGTCGTGGTTAACGCACTAGACAATGTAAATGCAAGGCTCTATGTCGATCAAAGGTGCTTATACTTCCAGAAACCACTTCTAGAATCTGGAACTCTTGGTGCTAAATGCAATACTCAAATGGTCATCCCTCACCTGACTGAAAACTATGGGGCATCAAGAGACCCCCCTGAGAAACAAGCGCCCATGTGCACTGTGCATTCGTTTCCACATAATATCGACCACTGTTTGACATGGGCTCGATCTGAGTTCGAGGGCTTGCTTGAGAAGACTCCTACTGATGTGAACGCTTATCTATCAAATCCCAGTGAATACACTTCTGCAATGATGAATGCTGGTGATGCTCAGTCTAGGGACACTTTAGAGCGCATTCTCGAGTGTCTTGATAGAGAAAGATGCGAGACGTTTGAAGACTGCATCACGTGGGCTCGCTTGAAGTTTGAAGATTATTTTTCGAACCGTGTGAAGCAGTTGATATACACATTTCCTGAAGATGCTGTAACTAGCAATGGGGCACCATTCTGGTCTGCCCCAAAGAGATTTCCCCATCCACTTCAGTTTTCAACTGCCGATCAAAGCTACCTTCATTTTGTTTTGGCGGCGGCTATACTAAGAGCAGAATCATATGCCATTCCGATTCCTGACTGGGTTAAGAACCCCACGAAATTGGCCGATGCAGTTGACCGAGTTATAGTACCAGATTTTATGCCCAAAAAAGATGCCAAGATTGTGACTGATGAGAAGGCAACCAGTCTCTCTACAGCATCAGTTGACGATGCAGCCGTTATCCACGACCTGGTGAACAAATTGGAGGATACACGCAGGAAGCTACCAGAGGGATTCAGGATGAAACCGATCCAGTTTGAGAAGGTCGTTCAGGATTTATTGATATTGTTATGTGTGATCAAAATTATTTCTGGAGATCTTAGAATCAATGGTGTAAATCTAACTACCTTAGTTTGGATGGTGCAGGATGATGATTCGAATTTCCATATGGATCTCATAGCTGGGCTGGCTAACATGAGAGCGAGGAATTACAGCATTCCTGAAGTAGACAAGTTGAAAGCCAAGTTCATTGCTGGAAGGATCATCCCCGCCATTGCTACCTCTACTGCAATGGCTACAGGTCTCGTCTGCCTCGAACTGTACAAAGTTCTAGATGGCGGTCACAAGGTGGAGGATTACCGTAACACGTTTGCGAACCTTGCATTGCCTCTGTTCTCCATGGCCGAGCCAGTCCCGCCCAAGGTCATCAAGCACCGGGACATGAGCTGGACTGTCTGGGACAGATGGATCATCAAAGACAACCCTACACTCAGACAACTTATTGAATGGTTGAAGAACAAGGGATTGAATGCCTACAGCATCTCGTGCGGTAGCTGTCTCCTGTACAATAGTATGTTCCCTCGACACAGAGATCGAATGGACAAGAAGGTAGTTGATTTAGCTCGAGATGTTGCCAAGGTGGAACTGCCTCCATACCGTCGACATTTGGATGTTGTCGTAGCATGCGAGGATGACGAGGATAATGATATTGACATCCCTCTGGTGTCGGTTTACTTCCGTTAG

Protein sequence

MWPELGFIGLPPIGDLLVFREVYGGVLDELFLGFVVLLVFFIMLRSFGVFSSLLHFMLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR
Homology
BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match: P93028 (Ubiquitin-activating enzyme E1 1 OS=Arabidopsis thaliana OX=3702 GN=UBA1 PE=1 SV=1)

HSP 1 Score: 1692.6 bits (4382), Expect = 0.0e+00
Identity = 834/1147 (72.71%), Postives = 956/1147 (83.35%), Query Frame = 0

Query: 79   SSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGASLI----MAS 138
            +S  N+ N+N++     +  KK+RID   +++ K +++    ++  + G S++    MA 
Sbjct: 6    ASEANDKNDNTIIGSDLASSKKRRIDFTESSSDKSSSILASGSSRGFHGDSVVQQIDMAF 65

Query: 139  ANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLP 198
             NSN  +IDEDLHSRQLAVYGRETMR+LFASNVLISGM GLGAEI               
Sbjct: 66   GNSNRQEIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEI--------------- 125

Query: 199  AAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHT 258
             AKN+ILAGVKSVTLHDE +VELWDLSSNFVFSE D+GKNRA AS QKLQDLNN+V+V +
Sbjct: 126  -AKNLILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSS 185

Query: 259  SMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQP 318
                L  E LS F+                       VVF+D  +++A+EF+D+CH+HQP
Sbjct: 186  LTKSLNKEDLSGFQ----------------------VVVFSDISMERAIEFDDYCHSHQP 245

Query: 319  PIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEF 378
            PIAF+K +VRGLFGSVFCDFGPEF V DV GE+PHTGIIASISN+N A +SCVDDERLEF
Sbjct: 246  PIAFVKADVRGLFGSVFCDFGPEFAVLDVDGEEPHTGIIASISNENQAFISCVDDERLEF 305

Query: 379  QDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKML 438
            +DGDLVVFSEV GMTELNDGKPR+IK+ R YSFTL+EDTTN+G+Y KGGIVTQVK+PK+L
Sbjct: 306  EDGDLVVFSEVEGMTELNDGKPRKIKSTRPYSFTLDEDTTNYGTYVKGGIVTQVKQPKLL 365

Query: 439  NFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLIS 498
            NFKPLREA+ DPGDFL SDFSKFDRPPLLHLAF ALD F  E GR PVAGSEEDAQKLIS
Sbjct: 366  NFKPLREALKDPGDFLFSDFSKFDRPPLLHLAFQALDHFKAEAGRFPVAGSEEDAQKLIS 425

Query: 499  VASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLV 558
            +A+ +N   GD +VE+++ KLLRHF+FGAKAVLNPMAAMFGGIV QEV+KACSGKFHPL 
Sbjct: 426  IATAINTGQGDLKVENVDQKLLRHFSFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 485

Query: 559  QFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEF 618
            QFFYFDS+ESLP+E +++SDF P NSRYDAQISVFG+K QKKLE+AKVF VGSGALGCEF
Sbjct: 486  QFFYFDSVESLPSEPVDSSDFAPRNSRYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEF 545

Query: 619  LKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHL 678
            LKNLALMGVSC ++GKLT+TDDD+IEKSNLSRQFLFRDWNIGQAKSTVAASAA  IN   
Sbjct: 546  LKNLALMGVSCGSQGKLTVTDDDIIEKSNLSRQFLFRDWNIGQAKSTVAASAAAVINPRF 605

Query: 679  NIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGA 738
            NIEALQNRV  ETENVFDD+FWENL VVVNALDNVNARLYVD RCLYFQKPLLESGTLG 
Sbjct: 606  NIEALQNRVGAETENVFDDAFWENLTVVVNALDNVNARLYVDSRCLYFQKPLLESGTLGT 665

Query: 739  KCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDV 798
            KCNTQ VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +V
Sbjct: 666  KCNTQSVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEV 725

Query: 799  NAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRV 858
            NAYLS+P EYT++MM+AGDAQ+RDTLERI+ECL++E+CETF+DC+TWARL+FEDYF NRV
Sbjct: 726  NAYLSSPVEYTNSMMSAGDAQARDTLERIVECLEKEKCETFQDCLTWARLRFEDYFVNRV 785

Query: 859  KQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPD 918
            KQLIYTFPEDA TS GAPFWSAPKRFP PLQ+S++D S L+F+ A AILRAE++ IPIP+
Sbjct: 786  KQLIYTFPEDAATSTGAPFWSAPKRFPRPLQYSSSDPSLLNFITATAILRAETFGIPIPE 845

Query: 919  WVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRR 978
            W KNP + A+AVDRVIVPDF P++DAKIVTDEKAT+L+TASVDDAAVI DL+ K++  R 
Sbjct: 846  WTKNPKEAAEAVDRVIVPDFEPRQDAKIVTDEKATTLTTASVDDAAVIDDLIAKIDQCRH 905

Query: 979  KLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLI 1038
             L   FRMKPIQFEK                                  DDD+N+HMD+I
Sbjct: 906  NLSPDFRMKPIQFEK----------------------------------DDDTNYHMDVI 965

Query: 1039 AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDY 1098
            AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVE Y
Sbjct: 966  AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEAY 1025

Query: 1099 RNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYS 1158
            RNTFANLALPLFSMAEP+PPKV+KHRDM+WTVWDRW++K NPTLR++++WL++KGL+AYS
Sbjct: 1026 RNTFANLALPLFSMAEPLPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYS 1080

Query: 1159 ISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIP 1218
            ISCGSCLL+NSMF RH++RMDKKVVDLARDVAKVELPPYR HLDVVVACED++DND+DIP
Sbjct: 1086 ISCGSCLLFNSMFTRHKERMDKKVVDLARDVAKVELPPYRNHLDVVVACEDEDDNDVDIP 1080

Query: 1219 LVSVYFR 1222
            LVS+YFR
Sbjct: 1146 LVSIYFR 1080

BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match: P92974 (Ubiquitin-activating enzyme E1 2 OS=Arabidopsis thaliana OX=3702 GN=UBA2 PE=1 SV=1)

HSP 1 Score: 1656.0 bits (4287), Expect = 0.0e+00
Identity = 822/1135 (72.42%), Postives = 932/1135 (82.11%), Query Frame = 0

Query: 95   ASLIKKQRID----SDSNANSKVAAVATGANNIVYDGASLIMASA-----NSNPPDIDED 154
            +S +KK+RID    +D +A +   + + G NN +  G   +M+ A     NSN  +IDED
Sbjct: 15   SSPMKKRRIDHTESADGSAINASNSSSIGLNNSI-GGNDTVMSMAEFGNDNSNNQEIDED 74

Query: 155  LHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVK 214
            LHSRQLAVYGRETMRKLFASNVLISGMQGLG EI                AKN+ILAGVK
Sbjct: 75   LHSRQLAVYGRETMRKLFASNVLISGMQGLGVEI----------------AKNIILAGVK 134

Query: 215  SVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLS 274
            SVTLHDE +VELWDLSSNFVF+E DIGKNRALAS  KLQ+LNN+V V T   KL  EQLS
Sbjct: 135  SVTLHDENVVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLTGKLTKEQLS 194

Query: 275  EFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRG 334
            +F+                       VVF D   +KA E +D+CH+HQPPIAFIK +VRG
Sbjct: 195  DFQ----------------------VVVFVDISFEKATEIDDYCHSHQPPIAFIKADVRG 254

Query: 335  LFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEV 394
            LFGS+FCDFGP FTV DV GE+PH+GIIAS+SN+NP  VSCVDDERLEF+DG+LVVFSEV
Sbjct: 255  LFGSLFCDFGPHFTVLDVDGEEPHSGIIASVSNENPGFVSCVDDERLEFEDGNLVVFSEV 314

Query: 395  HGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAIND 454
             GMTELNDGKPR+IKN + +SFTLEEDT+++G Y KGGIVTQVK+PK+LNFKPLREA+ D
Sbjct: 315  EGMTELNDGKPRKIKNVKPFSFTLEEDTSSYGQYMKGGIVTQVKQPKVLNFKPLREALKD 374

Query: 455  PGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGD 514
            PGDFLLSDFSKFDRPPLLHLAF ALD+F ++ GR P AGSEEDAQKL+ +A ++NE LGD
Sbjct: 375  PGDFLLSDFSKFDRPPLLHLAFQALDRFSSQAGRFPFAGSEEDAQKLVEIAVDINEGLGD 434

Query: 515  GRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESL 574
             R+ED+N KLLRH AFG++AVLNPMAAMFGGIV QEV+KACSGKFHP+ QFFYFDS+ESL
Sbjct: 435  ARLEDVNSKLLRHLAFGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPIFQFFYFDSVESL 494

Query: 575  PTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSC 634
            P E L+AS+FRP NSRYDAQISVFGS LQKKLE+A+VF+VG+GALGCEFLKNLALMGVSC
Sbjct: 495  PKEPLDASEFRPQNSRYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKNLALMGVSC 554

Query: 635  SNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSP 694
              +GKLT+TDDDVIEKSNLSRQFLFRDWNIGQAKSTVAA+AA  IN  LNI+ALQNRV P
Sbjct: 555  GTQGKLTVTDDDVIEKSNLSRQFLFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGP 614

Query: 695  ETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHL 754
            ETENVFDDSFWENL VVVNALDNV ARLYVD RC+YFQKPLLESGTLGAKCNTQMVIPHL
Sbjct: 615  ETENVFDDSFWENLTVVVNALDNVTARLYVDSRCVYFQKPLLESGTLGAKCNTQMVIPHL 674

Query: 755  TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYT 814
            TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +VNAYLS+P EY 
Sbjct: 675  TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSDPVEYM 734

Query: 815  SAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDA 874
             AM  AGDAQ+RDTL R++ECL++E+C +F+DCITWARL+FEDYF+NRVKQL YTFPEDA
Sbjct: 735  KAMRTAGDAQARDTLGRVVECLEKEKCNSFQDCITWARLRFEDYFANRVKQLCYTFPEDA 794

Query: 875  VTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADA 934
             TS GAPFWSAPKRFP PLQFS+ D S+++FV+AA+ILRAE++ IP P+W K    LA+A
Sbjct: 795  ATSTGAPFWSAPKRFPRPLQFSSTDLSHINFVMAASILRAETFGIPTPEWAKTRAGLAEA 854

Query: 935  VDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPI 994
            V+RVIVPDF PKKDA IVTDEKAT+LSTASVDDAAVI +L  KL   R  L   FRMK I
Sbjct: 855  VERVIVPDFEPKKDATIVTDEKATTLSTASVDDAAVIDELNAKLVRCRMSLQPEFRMKAI 914

Query: 995  QFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNY 1054
            QFEK                                  DDD+N+HMD+IAGLANMRARNY
Sbjct: 915  QFEK----------------------------------DDDTNYHMDMIAGLANMRARNY 974

Query: 1055 SIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPL 1114
            S+PEVDKLKAKFIAGRIIPAIATSTAMATG VCLE+YKVLDG HKVEDYRNTFANLALPL
Sbjct: 975  SVPEVDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGSHKVEDYRNTFANLALPL 1034

Query: 1115 FSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNS 1174
            FSMAEPVPPKV+KH+D SWTVWDRW+++ NPTLR+L++WLK KGLNAYSISCGS LLYNS
Sbjct: 1035 FSMAEPVPPKVVKHQDQSWTVWDRWVMRGNPTLRELLDWLKEKGLNAYSISCGSSLLYNS 1076

Query: 1175 MFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1221
            MF RH++RM+++VVDLARDVA VELP YRRH+DVVVACEDD D D+DIPLVSVYF
Sbjct: 1095 MFSRHKERMNRRVVDLARDVAGVELPAYRRHVDVVVACEDDNDADVDIPLVSVYF 1076

BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match: P20973 (Ubiquitin-activating enzyme E1 1 OS=Triticum aestivum OX=4565 GN=UBA1 PE=1 SV=1)

HSP 1 Score: 1630.9 bits (4222), Expect = 0.0e+00
Identity = 792/1082 (73.20%), Postives = 910/1082 (84.10%), Query Frame = 0

Query: 141  DIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVI 200
            +IDEDLHSRQLAVYGRETM++LF SNVL+SG+QGLGAEI                AKN++
Sbjct: 42   EIDEDLHSRQLAVYGRETMKRLFGSNVLVSGLQGLGAEI----------------AKNLV 101

Query: 201  LAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLV 260
            LAGVKSVTLHD+G VELWDLSSNF  SE+D+G+NRA A  QKLQ+LNN+V+V      L 
Sbjct: 102  LAGVKSVTLHDDGNVELWDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLT 161

Query: 261  MEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIK 320
             E LS+F+                      AVVFTD  LDKA+EF+D+CH+ QPPIAFIK
Sbjct: 162  KEHLSKFQ----------------------AVVFTDISLDKAIEFDDYCHSQQPPIAFIK 221

Query: 321  TEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLV 380
            +EVRGLFGSVFCDFGPEFTV DV GE+PHTGI+ASISNDNPALVSCVDDERLEFQDGDLV
Sbjct: 222  SEVRGLFGSVFCDFGPEFTVLDVDGEEPHTGIVASISNDNPALVSCVDDERLEFQDGDLV 281

Query: 381  VFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLR 440
            VFSEVHGMTELNDGKPR++KN R YSF LEEDT++FG+Y +GGIVTQVK PK++ FKPL+
Sbjct: 282  VFSEVHGMTELNDGKPRKVKNARPYSFFLEEDTSSFGAYVRGGIVTQVKPPKVIKFKPLK 341

Query: 441  EAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVN 500
            EA+++PG+FL+SDFSKF+RPPLLHLAF ALDKF TEL R PVAGS +D Q++I  A ++N
Sbjct: 342  EAMSEPGEFLMSDFSKFERPPLLHLAFQALDKFRTELSRFPVAGSTDDVQRVIEYAISIN 401

Query: 501  ESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFD 560
            ++LGD ++E+I+ KLL HFA G++AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFYFD
Sbjct: 402  DTLGDRKLEEIDKKLLHHFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFD 461

Query: 561  SLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLAL 620
            S+ESLP + LE  D +P NSRYDAQISVFGSKLQ KLE AK+FMVGSGALGCEFLKNLAL
Sbjct: 462  SVESLPVDPLEPGDLKPKNSRYDAQISVFGSKLQNKLEEAKIFMVGSGALGCEFLKNLAL 521

Query: 621  MGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQ 680
            MG+SCS  G LT+TDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AA+ IN  L++EALQ
Sbjct: 522  MGISCSQNGNLTLTDDDVIEKSNLSRQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQ 581

Query: 681  NRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQM 740
            NR SPETENVF+D+FWENL+ VVNALDNV AR+Y+D RC+YFQKPLLESGTLGAKCNTQM
Sbjct: 582  NRASPETENVFNDAFWENLDAVVNALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQM 641

Query: 741  VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSN 800
            VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPT+VNA+LSN
Sbjct: 642  VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSN 701

Query: 801  PSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYT 860
            P+ Y SA   AGDAQ+RD LER++ECLDR++CETF+D ITWARLKFEDYFSNRVKQL +T
Sbjct: 702  PTTYISAARTAGDAQARDQLERVIECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFT 761

Query: 861  FPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPT 920
            FPED++TS+GAPFWSAPKRFP P++FS++DQS L F+LAAAILRAE++ IPIP+W K P 
Sbjct: 762  FPEDSMTSSGAPFWSAPKRFPRPVEFSSSDQSQLSFILAAAILRAETFGIPIPEWAKTPN 821

Query: 921  KL-ADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEG 980
            KL A+AVD+VIVPDF PK+  KIVT EKATSLS+ASVDDAAVI +L+ KLE+  + LP G
Sbjct: 822  KLAAEAVDKVIVPDFQPKQGVKIVTHEKATSLSSASVDDAAVIEELIAKLEEVSKTLPSG 881

Query: 981  FRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLAN 1040
            F M PIQFEK                                  DDD+NFHMD+IAG AN
Sbjct: 882  FHMNPIQFEK----------------------------------DDDTNFHMDVIAGFAN 941

Query: 1041 MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA 1100
            MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRNTFA
Sbjct: 942  MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRNTFA 1001

Query: 1101 NLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGS 1160
            NLA+PLFS+AEPVPPK IKH+++SWTVWDRW +  N TLR+L+EWLK KGLNAYSISCG+
Sbjct: 1002 NLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYSISCGT 1051

Query: 1161 CLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVY 1220
             LLYNSMFPRH++R+D+KVVD+AR+VAK+E+P YRRHLDVVVACEDD+DND+DIPLVSVY
Sbjct: 1062 SLLYNSMFPRHKERLDRKVVDVAREVAKMEVPSYRRHLDVVVACEDDDDNDVDIPLVSVY 1051

Query: 1221 FR 1222
            FR
Sbjct: 1122 FR 1051

BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match: P31251 (Ubiquitin-activating enzyme E1 2 OS=Triticum aestivum OX=4565 GN=UBA2 PE=2 SV=1)

HSP 1 Score: 1628.2 bits (4215), Expect = 0.0e+00
Identity = 791/1082 (73.11%), Postives = 908/1082 (83.92%), Query Frame = 0

Query: 141  DIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVI 200
            +IDEDLHSRQLAVYGRETM+ LF SNVL+SG+QGLGAEI                AKN++
Sbjct: 42   EIDEDLHSRQLAVYGRETMKPLFGSNVLVSGLQGLGAEI----------------AKNLV 101

Query: 201  LAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLV 260
            LAGVKSVTLHD+G VELWDLSSNF  SE+D+G+NRA A  QKLQ+LNN+V+V      L 
Sbjct: 102  LAGVKSVTLHDDGNVELWDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLT 161

Query: 261  MEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIK 320
             E LS+F+                      AVVFTD  LDKA+EF+D+CH+HQPPIAFIK
Sbjct: 162  KEHLSKFQ----------------------AVVFTDISLDKAIEFDDYCHSHQPPIAFIK 221

Query: 321  TEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLV 380
            +EVRGLFGSVFCDFGPEFTV DV GE+PHTGI+ASISNDNPALVSCVDDERLEFQDGDLV
Sbjct: 222  SEVRGLFGSVFCDFGPEFTVLDVDGEEPHTGIVASISNDNPALVSCVDDERLEFQDGDLV 281

Query: 381  VFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLR 440
            VFSEVHGMTELNDGKPR++KN R YSF LEEDT++FG+Y +GGIVTQVK PK++ FKPL+
Sbjct: 282  VFSEVHGMTELNDGKPRKVKNARPYSFFLEEDTSSFGAYVRGGIVTQVKPPKVIKFKPLK 341

Query: 441  EAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVN 500
            EA+++PG+FL+SDFSKF+RPPLLHLAF ALDKF TEL R PVAGS +D Q++I  A ++N
Sbjct: 342  EAMSEPGEFLMSDFSKFERPPLLHLAFQALDKFRTELSRFPVAGSTDDVQRVIEYAISIN 401

Query: 501  ESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFD 560
            ++LGD ++E+I+ KLL HFA G++AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFYFD
Sbjct: 402  DTLGDRKLEEIDKKLLHHFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFD 461

Query: 561  SLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLAL 620
            S+ESLP + LE  D +P NSRYDAQISVFGS LQ KLE AK+FMVGSGALGCEFLKNLAL
Sbjct: 462  SVESLPVDPLEPGDLKPKNSRYDAQISVFGSTLQNKLEEAKIFMVGSGALGCEFLKNLAL 521

Query: 621  MGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQ 680
            MG+SCS  G LT+TDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AA+ IN  L++EALQ
Sbjct: 522  MGISCSQNGNLTVTDDDVIEKSNLSRQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQ 581

Query: 681  NRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQM 740
            NR SPETENVF+D+FWENL+ VVNALDNV AR+Y+D RC+YFQKPLLESGTLGAKCNTQM
Sbjct: 582  NRASPETENVFNDAFWENLDAVVNALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQM 641

Query: 741  VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSN 800
            VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPT+VNA+LSN
Sbjct: 642  VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSN 701

Query: 801  PSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYT 860
            P+ Y SA   AGDAQ+RD LER++ECLDR++CETF+D ITWARLKFEDYFSNRVKQL +T
Sbjct: 702  PTTYISAARTAGDAQARDQLERVIECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFT 761

Query: 861  FPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPT 920
            FPED++TS+GAPFWSAPKRFP P++FS++D S L F+LAAAILRAE++ IPI +W K P 
Sbjct: 762  FPEDSMTSSGAPFWSAPKRFPRPVEFSSSDPSQLSFILAAAILRAETFGIPISEWAKTPN 821

Query: 921  KL-ADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEG 980
            KL A+AVD+VIVPDF PK+  KIVTDEKATSLS+ASVDDAAVI +L+ KLE+  + LP G
Sbjct: 822  KLAAEAVDKVIVPDFQPKQGVKIVTDEKATSLSSASVDDAAVIEELIAKLEEVSKTLPSG 881

Query: 981  FRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLAN 1040
            F M PIQFEK                                  DDD+NFHMD+IAG AN
Sbjct: 882  FHMNPIQFEK----------------------------------DDDTNFHMDVIAGFAN 941

Query: 1041 MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA 1100
            MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRNTFA
Sbjct: 942  MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRNTFA 1001

Query: 1101 NLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGS 1160
            NLA+PLFS+AEPVPPK IKH+++SWTVWDRW +  N TLR+L+EWLK KGLNAYSISCG+
Sbjct: 1002 NLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYSISCGT 1051

Query: 1161 CLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVY 1220
             LLYNSMFPRH++R+D+KVVD+AR+VAK+E+P YRRHLDVVVACEDD+DND+DIPLVSVY
Sbjct: 1062 SLLYNSMFPRHKERLDRKVVDVAREVAKMEVPSYRRHLDVVVACEDDDDNDVDIPLVSVY 1051

Query: 1221 FR 1222
            FR
Sbjct: 1122 FR 1051

BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match: P31252 (Ubiquitin-activating enzyme E1 3 OS=Triticum aestivum OX=4565 GN=UBA3 PE=1 SV=1)

HSP 1 Score: 1559.7 bits (4037), Expect = 0.0e+00
Identity = 758/1083 (69.99%), Postives = 886/1083 (81.81%), Query Frame = 0

Query: 139  PPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKN 198
            P +IDEDLHSRQLAVYGRETMR+LFAS+VL+SG+ GLGAEI                AKN
Sbjct: 43   PQEIDEDLHSRQLAVYGRETMRRLFASDVLVSGLNGLGAEI----------------AKN 102

Query: 199  VILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTK 258
            + LAGVKSVT+HD   V++WDLS NF  SE DIGKNRA A   KLQ+LNN+V++     +
Sbjct: 103  LALAGVKSVTIHDVKTVKMWDLSGNFFLSEDDIGKNRAAACVAKLQELNNAVLISALTEE 162

Query: 259  LVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAF 318
            L  E LS+F+                      AVVFTD  LDKA EF+D+CHNHQPPI+F
Sbjct: 163  LTTEHLSKFQ----------------------AVVFTDIDLDKAYEFDDYCHNHQPPISF 222

Query: 319  IKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGD 378
            IK+EV GLFGSVFCDFGP+FTV DV GEDPHTGIIASISNDNPAL+SCVDDERLEFQDGD
Sbjct: 223  IKSEVCGLFGSVFCDFGPKFTVLDVDGEDPHTGIIASISNDNPALISCVDDERLEFQDGD 282

Query: 379  LVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKP 438
            LVVFSEVHGMTELNDGKPR++KN R +SF++EEDT+NFG Y KGGIVTQVKEPK+L FK 
Sbjct: 283  LVVFSEVHGMTELNDGKPRKVKNARPFSFSIEEDTSNFGIYVKGGIVTQVKEPKVLCFKA 342

Query: 439  LREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASN 498
            LR+A+ DPG+ LLSDFSKF+RPP+LHLAF ALDKF  + GR P AG EEDA   + +A+ 
Sbjct: 343  LRDAMTDPGEVLLSDFSKFERPPVLHLAFQALDKFKKDHGRCPAAGCEEDAHSFLKIAAA 402

Query: 499  VNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFY 558
            +NE+  D +++ I+ KL R FA G++AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFY
Sbjct: 403  INEASADRKLDTIDEKLFRQFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLNQFFY 462

Query: 559  FDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNL 618
            FDS+ESLPT  LE  D +P N+RYDAQ+SVFGSKLQKK+E A  F+VGSGALGCEFLKNL
Sbjct: 463  FDSVESLPTYPLEPQDLKPSNNRYDAQVSVFGSKLQKKMEEANTFVVGSGALGCEFLKNL 522

Query: 619  ALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEA 678
            ALMGVSCS++GKLTITDDD+IEKSNLSRQFLFRDWNIGQAKSTVAA+AA AIN  L+I+A
Sbjct: 523  ALMGVSCSSKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAATAASAINPSLHIDA 582

Query: 679  LQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNT 738
            LQNR  P+TENVF D+FWE L+VV+NALDNVNAR+Y+D RCLYFQKPLLESGTLGAKCN 
Sbjct: 583  LQNRACPDTENVFHDTFWEGLDVVINALDNVNARMYMDMRCLYFQKPLLESGTLGAKCNI 642

Query: 739  QMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYL 798
            QMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +VN++L
Sbjct: 643  QMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPNEVNSFL 702

Query: 799  SNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLI 858
            SNP++Y +AM  AGDAQ+R+ LER+ ECL+++RC TF+DCI+WARLKFEDYFSNRVKQL 
Sbjct: 703  SNPAQYAAAMRKAGDAQARELLERVSECLNKDRCSTFDDCISWARLKFEDYFSNRVKQLT 762

Query: 859  YTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKN 918
            +TFPEDA TS GAPFWSAPKRFP  LQFS ADQS+L+F+++A+ILRAES+ + IP+W K+
Sbjct: 763  FTFPEDAATSMGAPFWSAPKRFPRALQFSAADQSHLNFIMSASILRAESFGVAIPEWAKD 822

Query: 919  PTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPE 978
             +KLAD V+++ VP F PK+   IVTDEKA++LS+ SVDD AVI DL+ KL++  + L  
Sbjct: 823  TSKLADVVNKIAVPTFEPKQGVNIVTDEKASNLSSTSVDDVAVIEDLLAKLQEYAKMLLP 882

Query: 979  GFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLA 1038
            GF+MKPIQFEK                                  DDD+NFHMDLI+GLA
Sbjct: 883  GFQMKPIQFEK----------------------------------DDDTNFHMDLISGLA 942

Query: 1039 NMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTF 1098
            NMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKV+ G H VEDYRNTF
Sbjct: 943  NMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVIAGEHPVEDYRNTF 1002

Query: 1099 ANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCG 1158
            ANLALPLFSMAEPVPPKV+KH++ SWTVWDRW ++ N TL +L++W  +KGL AYSISCG
Sbjct: 1003 ANLALPLFSMAEPVPPKVMKHKETSWTVWDRWSVQGNLTLAELLQWFADKGLTAYSISCG 1053

Query: 1159 SCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSV 1218
            + LLYN+MF RH+DR+ KKVVD+AR+VAKV++P YRRHLD+ VACED+++ND+DIPLVSV
Sbjct: 1063 TSLLYNNMFARHKDRLTKKVVDIAREVAKVDVPEYRRHLDIGVACEDEDENDVDIPLVSV 1053

Query: 1219 YFR 1222
            YFR
Sbjct: 1123 YFR 1053

BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match: A0A6J1FYE8 (E1 ubiquitin-activating enzyme OS=Cucurbita moschata OX=3662 GN=LOC111448562 PE=3 SV=1)

HSP 1 Score: 2152.1 bits (5575), Expect = 0.0e+00
Identity = 1093/1165 (93.82%), Postives = 1093/1165 (93.82%), Query Frame = 0

Query: 57   MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 116
            MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV
Sbjct: 1    MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 60

Query: 117  ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 176
            ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61   ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120

Query: 177  AEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA 236
            AEI                AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA
Sbjct: 121  AEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA 180

Query: 237  LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTD 296
            LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFE                      AVVFTD
Sbjct: 181  LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFE----------------------AVVFTD 240

Query: 297  TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 356
            TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI
Sbjct: 241  TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 300

Query: 357  SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF 416
            SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF
Sbjct: 301  SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF 360

Query: 417  GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE 476
            GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE
Sbjct: 361  GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE 420

Query: 477  LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 536
            LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG
Sbjct: 421  LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 480

Query: 537  IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK 596
            IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK
Sbjct: 481  IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK 540

Query: 597  LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 656
            LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG
Sbjct: 541  LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 600

Query: 657  QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD 716
            QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD
Sbjct: 601  QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD 660

Query: 717  QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 776
            QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL
Sbjct: 661  QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 720

Query: 777  TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE 836
            TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE
Sbjct: 721  TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE 780

Query: 837  DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF 896
            DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF
Sbjct: 781  DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF 840

Query: 897  VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV 956
            VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV
Sbjct: 841  VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV 900

Query: 957  DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLT 1016
            DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK                           
Sbjct: 901  DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK--------------------------- 960

Query: 1017 TLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL 1076
                   DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL
Sbjct: 961  -------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL 1020

Query: 1077 VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP 1136
            VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP
Sbjct: 1021 VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP 1080

Query: 1137 TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH 1196
            TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH
Sbjct: 1081 TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH 1093

Query: 1197 LDVVVACEDDEDNDIDIPLVSVYFR 1222
            LDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 LDVVVACEDDEDNDIDIPLVSVYFR 1093

BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match: A0A6J1JC59 (E1 ubiquitin-activating enzyme OS=Cucurbita maxima OX=3661 GN=LOC111484417 PE=3 SV=1)

HSP 1 Score: 2135.1 bits (5531), Expect = 0.0e+00
Identity = 1088/1170 (92.99%), Postives = 1088/1170 (92.99%), Query Frame = 0

Query: 57   MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN-----NNSVRNEGASLIKKQRIDSDSNANS 116
            MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN     NNSVRNEGASLIKKQRIDSDSNANS
Sbjct: 1    MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNNNSNNSVRNEGASLIKKQRIDSDSNANS 60

Query: 117  KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 176
            KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG
Sbjct: 61   KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 120

Query: 177  MQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI 236
            MQGLGAEI                AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI
Sbjct: 121  MQGLGAEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI 180

Query: 237  GKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVA 296
            GKNRALASAQKLQDLNNSVIVHT  TKLV EQLSEFE                       
Sbjct: 181  GKNRALASAQKLQDLNNSVIVHTLTTKLVKEQLSEFE----------------------V 240

Query: 297  VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG 356
            VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG
Sbjct: 241  VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG 300

Query: 357  IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE 416
            IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE
Sbjct: 301  IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE 360

Query: 417  DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD 476
            DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD
Sbjct: 361  DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD 420

Query: 477  KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA 536
            KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA
Sbjct: 421  KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA 480

Query: 537  AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS 596
            AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS
Sbjct: 481  AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS 540

Query: 597  KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR 656
            KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR
Sbjct: 541  KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR 600

Query: 657  DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA 716
            DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA
Sbjct: 601  DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA 660

Query: 717  RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN 776
            RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN
Sbjct: 661  RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN 720

Query: 777  IDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRER 836
            IDHCLTWARSEFEGLLEKTPTDVNAYL NPSEYTSAMMNAGDAQSRDTLERILECLDRER
Sbjct: 721  IDHCLTWARSEFEGLLEKTPTDVNAYLLNPSEYTSAMMNAGDAQSRDTLERILECLDRER 780

Query: 837  CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ 896
            CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ
Sbjct: 781  CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ 840

Query: 897  SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL 956
            SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL
Sbjct: 841  SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL 900

Query: 957  STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRIN 1016
            STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK                      
Sbjct: 901  STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK---------------------- 960

Query: 1017 GVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA 1076
                        DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA
Sbjct: 961  ------------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA 1020

Query: 1077 MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI 1136
            MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI
Sbjct: 1021 MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI 1080

Query: 1137 IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP 1196
            IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP
Sbjct: 1081 IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP 1098

Query: 1197 PYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
            PYRRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 PYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1098

BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match: A0A5A7VQ32 (E1 ubiquitin-activating enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold615G00120 PE=3 SV=1)

HSP 1 Score: 2108.2 bits (5461), Expect = 0.0e+00
Identity = 1070/1224 (87.42%), Postives = 1110/1224 (90.69%), Query Frame = 0

Query: 1    MWPELGFIGLPPIGDLLVFREVYGGVLDELFLGFVVLLVFFIMLRSFGVFSSLLHFMLPR 60
            M P LGF+GL PI  LL+F  VYG  ++  FLGF+V  V F++LR FG+FSSLLHFMLPR
Sbjct: 1    MLPSLGFMGLLPIALLLLFHGVYGYDMNGFFLGFLVFFVSFLILRCFGIFSSLLHFMLPR 60

Query: 61   KRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAVA--- 120
            KRAGEEGV VEE+TDN S+S   NNNN+SV+N GASLIKKQRIDSDSN+N+ VAAVA   
Sbjct: 61   KRAGEEGVVVEEETDNGSTSVVFNNNNSSVQNVGASLIKKQRIDSDSNSNTNVAAVATVP 120

Query: 121  TGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGA 180
            T ANNIV D ASLIMAS NSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGA
Sbjct: 121  TTANNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGA 180

Query: 181  EIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRAL 240
            EI                AKNVILAGVKSVTLHDEG+VELWDLSSNFVFSESD+GKNRAL
Sbjct: 181  EI----------------AKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDVGKNRAL 240

Query: 241  ASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDT 300
            ASAQKLQDLNNSVIVHT  TKLV EQLS+FE                       VVFTDT
Sbjct: 241  ASAQKLQDLNNSVIVHTLTTKLVKEQLSDFE----------------------VVVFTDT 300

Query: 301  GLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASIS 360
             LDKAMEF+DFCHNHQPPI+FIK+EVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASIS
Sbjct: 301  SLDKAMEFDDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASIS 360

Query: 361  NDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFG 420
            NDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT NFG
Sbjct: 361  NDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTANFG 420

Query: 421  SYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTEL 480
            SYEKGGIVTQVK+PK+LNFKPLREAINDPGDFLLSDFSKFDRPPL+HLAFLALDKFVTEL
Sbjct: 421  SYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTEL 480

Query: 481  GRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGI 540
            GRLP+AGSEEDAQKLISVAS +NESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGI
Sbjct: 481  GRLPIAGSEEDAQKLISVASTINESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGI 540

Query: 541  VAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKL 600
            VAQEVLKACSGKFHPLVQFFYFDS+ESLPTESL+ASDFRPLNSRYDAQISVFGSKLQKKL
Sbjct: 541  VAQEVLKACSGKFHPLVQFFYFDSVESLPTESLDASDFRPLNSRYDAQISVFGSKLQKKL 600

Query: 601  ENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQ 660
            ENAKVFMVGSGALGCEFLKNLALMGVSCS+EGKLTITDDDVIEKSNLSRQFLFRDWNIGQ
Sbjct: 601  ENAKVFMVGSGALGCEFLKNLALMGVSCSSEGKLTITDDDVIEKSNLSRQFLFRDWNIGQ 660

Query: 661  AKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQ 720
            AKSTVAA+AAVAIN+HLNIEALQNRVSPETENVFDDSFWENL+VVVNALDNVNARLYVDQ
Sbjct: 661  AKSTVAAAAAVAINRHLNIEALQNRVSPETENVFDDSFWENLSVVVNALDNVNARLYVDQ 720

Query: 721  RCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLT 780
            RCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLT
Sbjct: 721  RCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLT 780

Query: 781  WARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFED 840
            WARSEFEGLLEKTPTDVNAYLSNP+EYTSAMMNAGDAQSRDTLERILECLDRERCETFED
Sbjct: 781  WARSEFEGLLEKTPTDVNAYLSNPNEYTSAMMNAGDAQSRDTLERILECLDRERCETFED 840

Query: 841  CITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFV 900
            CITWARLKFEDYF+NRVKQLIYTFPEDA TSNGAPFWSAPKRFPHPLQFST+DQS+L FV
Sbjct: 841  CITWARLKFEDYFANRVKQLIYTFPEDAQTSNGAPFWSAPKRFPHPLQFSTSDQSHLQFV 900

Query: 901  LAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVD 960
            LAAAILRAESYAIP+PDWVKNP KLADA+DRVIVPDFMPKKDAKIVTDEKATSLSTASVD
Sbjct: 901  LAAAILRAESYAIPVPDWVKNPRKLADAIDRVIVPDFMPKKDAKIVTDEKATSLSTASVD 960

Query: 961  DAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTT 1020
            DAAVIHDL  KLE+TRRKLPEGFRMKPIQFEK                            
Sbjct: 961  DAAVIHDLAGKLEETRRKLPEGFRMKPIQFEK---------------------------- 1020

Query: 1021 LVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLV 1080
                  DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLV
Sbjct: 1021 ------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLV 1080

Query: 1081 CLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPT 1140
            CLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPT
Sbjct: 1081 CLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPT 1140

Query: 1141 LRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHL 1200
            LR+LI+WLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP YRRHL
Sbjct: 1141 LRELIDWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPLYRRHL 1152

Query: 1201 DVVVACEDDEDNDIDIPLVSVYFR 1222
            DVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1201 DVVVACEDDEDNDIDIPLVSVYFR 1152

BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match: A0A0A0KE12 (E1 ubiquitin-activating enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G181590 PE=3 SV=1)

HSP 1 Score: 2060.4 bits (5337), Expect = 0.0e+00
Identity = 1046/1186 (88.20%), Postives = 1081/1186 (91.15%), Query Frame = 0

Query: 41   FIMLRSFGVF--SSLLHFMLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLI 100
            F+   SF V   +SLLHFMLPRKRAGEEGV VEE+TDN S+S   NNNN+SV+N GAS I
Sbjct: 48   FLPSSSFTVLIGTSLLHFMLPRKRAGEEGVVVEEETDNGSTSVVINNNNSSVQNVGASFI 107

Query: 101  KKQRIDSDSNANSKVAAVA---TGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYG 160
            KKQRIDSDSN+N+ VAAVA   T A+NIV D ASLIMAS NSNPPDIDEDLHSRQLAVYG
Sbjct: 108  KKQRIDSDSNSNTNVAAVATVPTTASNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYG 167

Query: 161  RETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIV 220
            RETMRKLFASNVLISGMQGLGAEI                AKNVILAGVKSVTLHDEG+V
Sbjct: 168  RETMRKLFASNVLISGMQGLGAEI----------------AKNVILAGVKSVTLHDEGVV 227

Query: 221  ELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIH 280
            ELWDLSSNFVFSESD+GKNRALASAQKLQDLNNSVIVHT  +KLV EQLS+FE       
Sbjct: 228  ELWDLSSNFVFSESDVGKNRALASAQKLQDLNNSVIVHTLTSKLVKEQLSDFE------- 287

Query: 281  GRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFG 340
                            VVFTDT LDKAMEFNDFCHNHQPPI+FIK+EVRGLFGSVFCDFG
Sbjct: 288  ---------------VVVFTDTSLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFG 347

Query: 341  PEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGK 400
            PEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGK
Sbjct: 348  PEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGK 407

Query: 401  PRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFS 460
            PRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVK+PK+LNFKPLREAINDPGDFLLSDFS
Sbjct: 408  PRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFS 467

Query: 461  KFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKL 520
            KFDRPPL+HLAFLALDKFVTELGRLPVAGSEEDAQKLISVASN+NESLGDGRVEDINPKL
Sbjct: 468  KFDRPPLIHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNINESLGDGRVEDINPKL 527

Query: 521  LRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDF 580
            LRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDS+ESLPTESL+AS+F
Sbjct: 528  LRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTESLDASEF 587

Query: 581  RPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITD 640
            RPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCS+EGKLTITD
Sbjct: 588  RPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSSEGKLTITD 647

Query: 641  DDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSF 700
            DDVIEKSNLSRQFLFRDWNIGQAKSTVAA+AAVAIN+HLNIEALQNRVSPETENVFDDSF
Sbjct: 648  DDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAAVAINRHLNIEALQNRVSPETENVFDDSF 707

Query: 701  WENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDP 760
            WENL+VVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDP
Sbjct: 708  WENLSVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDP 767

Query: 761  PEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQ 820
            PEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQ
Sbjct: 768  PEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQ 827

Query: 821  SRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWS 880
            SRDTLERILECLDRERCETFEDCITWARLKFEDYF+NRVKQLIYTFPEDA TSNGAPFWS
Sbjct: 828  SRDTLERILECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAQTSNGAPFWS 887

Query: 881  APKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFM 940
            APKRFPHPLQFST+DQS+L FVLAAAILR+ESYAIPIPDWVKNP KLADA+DR+IVPDFM
Sbjct: 888  APKRFPHPLQFSTSDQSHLQFVLAAAILRSESYAIPIPDWVKNPRKLADAIDRIIVPDFM 947

Query: 941  PKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLL 1000
            PKKDAKIVTDEKATSLSTASVDDAAVIHDL  KLE+T RKLPEGFRMKPIQFEK      
Sbjct: 948  PKKDAKIVTDEKATSLSTASVDDAAVIHDLAGKLEETCRKLPEGFRMKPIQFEK------ 1007

Query: 1001 ILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKA 1060
                                        DDDSNFHMDLIAGLANMRARNYSIPEVDKLKA
Sbjct: 1008 ----------------------------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKA 1067

Query: 1061 KFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPK 1120
            KFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPK
Sbjct: 1068 KFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPK 1127

Query: 1121 VIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMD 1180
            VIKHRDMSWTVWDRWIIKDNPTLR+LI+WLKNKGLNAYSISCGSCLLYNSMFPRHRDRMD
Sbjct: 1128 VIKHRDMSWTVWDRWIIKDNPTLRELIDWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMD 1161

Query: 1181 KKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
            KKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1188 KKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1161

BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match: A0A1S3CNI4 (E1 ubiquitin-activating enzyme OS=Cucumis melo OX=3656 GN=LOC103502928 PE=3 SV=1)

HSP 1 Score: 2050.0 bits (5310), Expect = 0.0e+00
Identity = 1037/1168 (88.78%), Postives = 1069/1168 (91.52%), Query Frame = 0

Query: 57   MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 116
            MLPRKRAGEEGV VEE+TDN S+S   NNNN+SV+N GASLIKKQRIDSDSN+N+ VAAV
Sbjct: 1    MLPRKRAGEEGVVVEEETDNGSTSVVFNNNNSSVQNVGASLIKKQRIDSDSNSNTNVAAV 60

Query: 117  A---TGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 176
            A   T ANNIV D ASLIMAS NSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ
Sbjct: 61   ATVPTTANNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 120

Query: 177  GLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGK 236
            GLGAEI                AKNVILAGVKSVTLHDEG+VELWDLSSNFVFSESD+GK
Sbjct: 121  GLGAEI----------------AKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDVGK 180

Query: 237  NRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVV 296
            NRALASAQKLQDLNNSVIVHT  TKLV EQLS+FE                       VV
Sbjct: 181  NRALASAQKLQDLNNSVIVHTLTTKLVKEQLSDFE----------------------VVV 240

Query: 297  FTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 356
            FTDT LDKAMEF+DFCHNHQPPI+FIK+EVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII
Sbjct: 241  FTDTSLDKAMEFDDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 300

Query: 357  ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT 416
            ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT
Sbjct: 301  ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT 360

Query: 417  TNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKF 476
             NFGSYEKGGIVTQVK+PK+LNFKPLREAINDPGDFLLSDFSKFDRPPL+HLAFLALDKF
Sbjct: 361  ANFGSYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKF 420

Query: 477  VTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 536
            VTELGRLP+AGSEEDAQKLISVAS +NESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM
Sbjct: 421  VTELGRLPIAGSEEDAQKLISVASTINESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 480

Query: 537  FGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKL 596
            FGGIVAQEVLKACSGKFHPLVQFFYFDS+ESLPTESL+ASDFRPLNSRYDAQISVFGSKL
Sbjct: 481  FGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTESLDASDFRPLNSRYDAQISVFGSKL 540

Query: 597  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 656
            QKKLENAKVFMVGSGALGCEFLKNLALMGVSCS+EGKLTITDDDVIEKSNLSRQFLFRDW
Sbjct: 541  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSSEGKLTITDDDVIEKSNLSRQFLFRDW 600

Query: 657  NIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARL 716
            NIGQAKSTVAA+AAVAIN+HLNIEALQNRVSPETENVFDDSFWENL+VVVNALDNVNARL
Sbjct: 601  NIGQAKSTVAAAAAVAINRHLNIEALQNRVSPETENVFDDSFWENLSVVVNALDNVNARL 660

Query: 717  YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 776
            YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID
Sbjct: 661  YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 720

Query: 777  HCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCE 836
            HCLTWARSEFEGLLEKTPTDVNAYLSNP+EYTSAMMNAGDAQSRDTLERILECLDRERCE
Sbjct: 721  HCLTWARSEFEGLLEKTPTDVNAYLSNPNEYTSAMMNAGDAQSRDTLERILECLDRERCE 780

Query: 837  TFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSY 896
            TFEDCITWARLKFEDYF+NRVKQLIYTFPEDA TSNGAPFWSAPKRFPHPLQFST+DQS+
Sbjct: 781  TFEDCITWARLKFEDYFANRVKQLIYTFPEDAQTSNGAPFWSAPKRFPHPLQFSTSDQSH 840

Query: 897  LHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLST 956
            L FVLAAAILRAESYAIP+PDWVKNP KLADA+DRVIVPDFMPKKDAKIVTDEKATSLST
Sbjct: 841  LQFVLAAAILRAESYAIPVPDWVKNPRKLADAIDRVIVPDFMPKKDAKIVTDEKATSLST 900

Query: 957  ASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGV 1016
            ASVDDAAVIHDL  KLE+TRRKLPEGFRMKPIQFEK                        
Sbjct: 901  ASVDDAAVIHDLAGKLEETRRKLPEGFRMKPIQFEK------------------------ 960

Query: 1017 NLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA 1076
                      DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA
Sbjct: 961  ----------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA 1020

Query: 1077 TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK 1136
            TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK
Sbjct: 1021 TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK 1080

Query: 1137 DNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPY 1196
            DNPTLR+LI+WLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP Y
Sbjct: 1081 DNPTLRELIDWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPLY 1096

Query: 1197 RRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
            RRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 RRHLDVVVACEDDEDNDIDIPLVSVYFR 1096

BLAST of CmoCh20G003570 vs. NCBI nr
Match: KAG7010491.1 (Ubiquitin-activating enzyme E1 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2198.7 bits (5696), Expect = 0.0e+00
Identity = 1123/1213 (92.58%), Postives = 1129/1213 (93.08%), Query Frame = 0

Query: 12   PIGDLLVFREVYGGVLDELFLGFVVLLVFFIMLRSFGVFSSLLHFMLPRKRAGEEGVAVE 71
            P     +  EVYGGVLDELFLGFVVLLVFFIMLRSFGVFSSLLHFMLPRKRAGEEGVAVE
Sbjct: 42   PSSSFTLLIEVYGGVLDELFLGFVVLLVFFIMLRSFGVFSSLLHFMLPRKRAGEEGVAVE 101

Query: 72   EKTDNSSSSN---NNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGA 131
            EKTDNSSSS+   NNNN+NNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGA
Sbjct: 102  EKTDNSSSSSNNINNNNSNNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGA 161

Query: 132  SLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLL 191
            SLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEI         
Sbjct: 162  SLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEI--------- 221

Query: 192  YFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNN 251
                   AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNN
Sbjct: 222  -------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNN 281

Query: 252  SVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDF 311
            SVIVHT  T+LV+EQLS+FE                      AVVFTDTGLDKAMEFNDF
Sbjct: 282  SVIVHTLTTELVIEQLSKFE----------------------AVVFTDTGLDKAMEFNDF 341

Query: 312  CHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVD 371
            CHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVD
Sbjct: 342  CHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVD 401

Query: 372  DERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQV 431
            DERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQV
Sbjct: 402  DERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQV 461

Query: 432  KEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEED 491
            KEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEED
Sbjct: 462  KEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEED 521

Query: 492  AQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSG 551
            AQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSG
Sbjct: 522  AQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSG 581

Query: 552  KFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSG 611
            KFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSG
Sbjct: 582  KFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSG 641

Query: 612  ALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAV 671
            ALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAV
Sbjct: 642  ALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAV 701

Query: 672  AINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLE 731
            AINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLE
Sbjct: 702  AINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLE 761

Query: 732  SGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLE 791
            SGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLE
Sbjct: 762  SGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLE 821

Query: 792  KTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFED 851
            KTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFED
Sbjct: 822  KTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFED 881

Query: 852  YFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESY 911
            YFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESY
Sbjct: 882  YFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESY 941

Query: 912  AIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNK 971
            AIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNK
Sbjct: 942  AIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNK 1001

Query: 972  LEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSN 1031
            LEDTRRKLPEGFRMKPIQFEK                                  DDDSN
Sbjct: 1002 LEDTRRKLPEGFRMKPIQFEK----------------------------------DDDSN 1061

Query: 1032 FHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1091
            FHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG
Sbjct: 1062 FHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1121

Query: 1092 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNK 1151
            HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNK
Sbjct: 1122 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNK 1181

Query: 1152 GLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDED 1211
            GLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDED
Sbjct: 1182 GLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDED 1182

Query: 1212 NDIDIPLVSVYFR 1222
            NDIDIPLVSVYFR
Sbjct: 1242 NDIDIPLVSVYFR 1182

BLAST of CmoCh20G003570 vs. NCBI nr
Match: XP_022943999.1 (ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita moschata] >XP_022944000.1 ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 2152.1 bits (5575), Expect = 0.0e+00
Identity = 1093/1165 (93.82%), Postives = 1093/1165 (93.82%), Query Frame = 0

Query: 57   MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 116
            MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV
Sbjct: 1    MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 60

Query: 117  ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 176
            ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61   ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120

Query: 177  AEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA 236
            AEI                AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA
Sbjct: 121  AEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA 180

Query: 237  LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTD 296
            LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFE                      AVVFTD
Sbjct: 181  LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFE----------------------AVVFTD 240

Query: 297  TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 356
            TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI
Sbjct: 241  TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 300

Query: 357  SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF 416
            SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF
Sbjct: 301  SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF 360

Query: 417  GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE 476
            GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE
Sbjct: 361  GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE 420

Query: 477  LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 536
            LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG
Sbjct: 421  LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 480

Query: 537  IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK 596
            IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK
Sbjct: 481  IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK 540

Query: 597  LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 656
            LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG
Sbjct: 541  LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 600

Query: 657  QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD 716
            QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD
Sbjct: 601  QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD 660

Query: 717  QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 776
            QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL
Sbjct: 661  QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 720

Query: 777  TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE 836
            TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE
Sbjct: 721  TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE 780

Query: 837  DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF 896
            DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF
Sbjct: 781  DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF 840

Query: 897  VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV 956
            VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV
Sbjct: 841  VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV 900

Query: 957  DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLT 1016
            DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK                           
Sbjct: 901  DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK--------------------------- 960

Query: 1017 TLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL 1076
                   DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL
Sbjct: 961  -------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL 1020

Query: 1077 VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP 1136
            VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP
Sbjct: 1021 VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP 1080

Query: 1137 TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH 1196
            TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH
Sbjct: 1081 TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH 1093

Query: 1197 LDVVVACEDDEDNDIDIPLVSVYFR 1222
            LDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 LDVVVACEDDEDNDIDIPLVSVYFR 1093

BLAST of CmoCh20G003570 vs. NCBI nr
Match: KAG6570640.1 (Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2142.5 bits (5550), Expect = 0.0e+00
Identity = 1090/1173 (92.92%), Postives = 1095/1173 (93.35%), Query Frame = 0

Query: 52   SLLHFMLPRKRAGEEGVAVEEKTDNSSSS---NNNNNNNNSVRNEGASLIKKQRIDSDSN 111
            SLLHFMLPRKRAGEEGVAVEEKTDNSSSS   NNNNN+NNSVRNEGASLIKKQRIDSDSN
Sbjct: 584  SLLHFMLPRKRAGEEGVAVEEKTDNSSSSSNNNNNNNSNNSVRNEGASLIKKQRIDSDSN 643

Query: 112  ANSKVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVL 171
            ANSKVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVL
Sbjct: 644  ANSKVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVL 703

Query: 172  ISGMQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSE 231
            ISGMQGLGAEI                AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSE
Sbjct: 704  ISGMQGLGAEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSE 763

Query: 232  SDIGKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTT 291
            SDIGKNRALASAQKLQDLNNSVIVHT  T+LV+EQLS+FE                    
Sbjct: 764  SDIGKNRALASAQKLQDLNNSVIVHTLTTELVIEQLSKFE-------------------- 823

Query: 292  MVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDP 351
              AVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDP
Sbjct: 824  --AVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDP 883

Query: 352  HTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFT 411
            HTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFT
Sbjct: 884  HTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFT 943

Query: 412  LEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFL 471
            LEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFL
Sbjct: 944  LEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFL 1003

Query: 472  ALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLN 531
            ALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLN
Sbjct: 1004 ALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLN 1063

Query: 532  PMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISV 591
            PMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISV
Sbjct: 1064 PMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISV 1123

Query: 592  FGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQF 651
            FGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQF
Sbjct: 1124 FGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQF 1183

Query: 652  LFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDN 711
            LFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDN
Sbjct: 1184 LFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDN 1243

Query: 712  VNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSF 771
            VNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSF
Sbjct: 1244 VNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSF 1303

Query: 772  PHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLD 831
            PHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLD
Sbjct: 1304 PHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLD 1363

Query: 832  RERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFST 891
            RERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFST
Sbjct: 1364 RERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFST 1423

Query: 892  ADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKA 951
            ADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKA
Sbjct: 1424 ADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKA 1483

Query: 952  TSLSTASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDL 1011
            TSLSTASVDDAA+IHDLVNKLEDT RKLPEGFRMKPIQFEK                   
Sbjct: 1484 TSLSTASVDDAAIIHDLVNKLEDTSRKLPEGFRMKPIQFEK------------------- 1543

Query: 1012 RINGVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIAT 1071
                           DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIAT
Sbjct: 1544 ---------------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIAT 1603

Query: 1072 STAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWD 1131
            STAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWD
Sbjct: 1604 STAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWD 1663

Query: 1132 RWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKV 1191
            RWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKV
Sbjct: 1664 RWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKV 1684

Query: 1192 ELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
            ELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1724 ELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1684

BLAST of CmoCh20G003570 vs. NCBI nr
Match: XP_023512974.1 (ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512976.1 ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2138.2 bits (5539), Expect = 0.0e+00
Identity = 1089/1168 (93.24%), Postives = 1090/1168 (93.32%), Query Frame = 0

Query: 57   MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN---NNSVRNEGASLIKKQRIDSDSNANSKV 116
            MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN   NNSVRNEGASLIKKQRIDSDSNANSKV
Sbjct: 1    MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNSNSNNSVRNEGASLIKKQRIDSDSNANSKV 60

Query: 117  AAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 176
            AAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ
Sbjct: 61   AAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 120

Query: 177  GLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGK 236
            GLGAEI                AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGK
Sbjct: 121  GLGAEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGK 180

Query: 237  NRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVV 296
            NRALASAQKLQDLNNSVIVHTSMTKLV EQLSEFE                       VV
Sbjct: 181  NRALASAQKLQDLNNSVIVHTSMTKLVKEQLSEFE----------------------VVV 240

Query: 297  FTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 356
            FTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII
Sbjct: 241  FTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 300

Query: 357  ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT 416
            ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT
Sbjct: 301  ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT 360

Query: 417  TNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKF 476
            TNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKF
Sbjct: 361  TNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKF 420

Query: 477  VTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 536
            VTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM
Sbjct: 421  VTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 480

Query: 537  FGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKL 596
            FGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKL
Sbjct: 481  FGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKL 540

Query: 597  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 656
            QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW
Sbjct: 541  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 600

Query: 657  NIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARL 716
            NIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARL
Sbjct: 601  NIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARL 660

Query: 717  YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 776
            YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID
Sbjct: 661  YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 720

Query: 777  HCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCE 836
            HCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCE
Sbjct: 721  HCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCE 780

Query: 837  TFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSY 896
            TFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSY
Sbjct: 781  TFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSY 840

Query: 897  LHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLST 956
            LHFVLAAAILRAESYAI IPDWVKNPTKLADAVDRV+VPDFMPKKDAKIVTDEKATSLST
Sbjct: 841  LHFVLAAAILRAESYAIRIPDWVKNPTKLADAVDRVMVPDFMPKKDAKIVTDEKATSLST 900

Query: 957  ASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGV 1016
            ASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK                        
Sbjct: 901  ASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK------------------------ 960

Query: 1017 NLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA 1076
                      DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA
Sbjct: 961  ----------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA 1020

Query: 1077 TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK 1136
            TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK
Sbjct: 1021 TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK 1080

Query: 1137 DNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPY 1196
            DNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPY
Sbjct: 1081 DNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPY 1096

Query: 1197 RRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
            RRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 RRHLDVVVACEDDEDNDIDIPLVSVYFR 1096

BLAST of CmoCh20G003570 vs. NCBI nr
Match: XP_022986756.1 (ubiquitin-activating enzyme E1 1-like [Cucurbita maxima])

HSP 1 Score: 2135.1 bits (5531), Expect = 0.0e+00
Identity = 1088/1170 (92.99%), Postives = 1088/1170 (92.99%), Query Frame = 0

Query: 57   MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN-----NNSVRNEGASLIKKQRIDSDSNANS 116
            MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN     NNSVRNEGASLIKKQRIDSDSNANS
Sbjct: 1    MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNNNSNNSVRNEGASLIKKQRIDSDSNANS 60

Query: 117  KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 176
            KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG
Sbjct: 61   KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 120

Query: 177  MQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI 236
            MQGLGAEI                AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI
Sbjct: 121  MQGLGAEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI 180

Query: 237  GKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVA 296
            GKNRALASAQKLQDLNNSVIVHT  TKLV EQLSEFE                       
Sbjct: 181  GKNRALASAQKLQDLNNSVIVHTLTTKLVKEQLSEFE----------------------V 240

Query: 297  VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG 356
            VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG
Sbjct: 241  VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG 300

Query: 357  IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE 416
            IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE
Sbjct: 301  IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE 360

Query: 417  DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD 476
            DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD
Sbjct: 361  DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD 420

Query: 477  KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA 536
            KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA
Sbjct: 421  KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA 480

Query: 537  AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS 596
            AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS
Sbjct: 481  AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS 540

Query: 597  KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR 656
            KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR
Sbjct: 541  KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR 600

Query: 657  DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA 716
            DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA
Sbjct: 601  DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA 660

Query: 717  RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN 776
            RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN
Sbjct: 661  RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN 720

Query: 777  IDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRER 836
            IDHCLTWARSEFEGLLEKTPTDVNAYL NPSEYTSAMMNAGDAQSRDTLERILECLDRER
Sbjct: 721  IDHCLTWARSEFEGLLEKTPTDVNAYLLNPSEYTSAMMNAGDAQSRDTLERILECLDRER 780

Query: 837  CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ 896
            CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ
Sbjct: 781  CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ 840

Query: 897  SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL 956
            SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL
Sbjct: 841  SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL 900

Query: 957  STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRIN 1016
            STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK                      
Sbjct: 901  STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK---------------------- 960

Query: 1017 GVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA 1076
                        DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA
Sbjct: 961  ------------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA 1020

Query: 1077 MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI 1136
            MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI
Sbjct: 1021 MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI 1080

Query: 1137 IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP 1196
            IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP
Sbjct: 1081 IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP 1098

Query: 1197 PYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
            PYRRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 PYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1098

BLAST of CmoCh20G003570 vs. TAIR 10
Match: AT2G30110.1 (ubiquitin-activating enzyme 1 )

HSP 1 Score: 1692.6 bits (4382), Expect = 0.0e+00
Identity = 834/1147 (72.71%), Postives = 956/1147 (83.35%), Query Frame = 0

Query: 79   SSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGASLI----MAS 138
            +S  N+ N+N++     +  KK+RID   +++ K +++    ++  + G S++    MA 
Sbjct: 6    ASEANDKNDNTIIGSDLASSKKRRIDFTESSSDKSSSILASGSSRGFHGDSVVQQIDMAF 65

Query: 139  ANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLP 198
             NSN  +IDEDLHSRQLAVYGRETMR+LFASNVLISGM GLGAEI               
Sbjct: 66   GNSNRQEIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEI--------------- 125

Query: 199  AAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHT 258
             AKN+ILAGVKSVTLHDE +VELWDLSSNFVFSE D+GKNRA AS QKLQDLNN+V+V +
Sbjct: 126  -AKNLILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSS 185

Query: 259  SMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQP 318
                L  E LS F+                       VVF+D  +++A+EF+D+CH+HQP
Sbjct: 186  LTKSLNKEDLSGFQ----------------------VVVFSDISMERAIEFDDYCHSHQP 245

Query: 319  PIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEF 378
            PIAF+K +VRGLFGSVFCDFGPEF V DV GE+PHTGIIASISN+N A +SCVDDERLEF
Sbjct: 246  PIAFVKADVRGLFGSVFCDFGPEFAVLDVDGEEPHTGIIASISNENQAFISCVDDERLEF 305

Query: 379  QDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKML 438
            +DGDLVVFSEV GMTELNDGKPR+IK+ R YSFTL+EDTTN+G+Y KGGIVTQVK+PK+L
Sbjct: 306  EDGDLVVFSEVEGMTELNDGKPRKIKSTRPYSFTLDEDTTNYGTYVKGGIVTQVKQPKLL 365

Query: 439  NFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLIS 498
            NFKPLREA+ DPGDFL SDFSKFDRPPLLHLAF ALD F  E GR PVAGSEEDAQKLIS
Sbjct: 366  NFKPLREALKDPGDFLFSDFSKFDRPPLLHLAFQALDHFKAEAGRFPVAGSEEDAQKLIS 425

Query: 499  VASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLV 558
            +A+ +N   GD +VE+++ KLLRHF+FGAKAVLNPMAAMFGGIV QEV+KACSGKFHPL 
Sbjct: 426  IATAINTGQGDLKVENVDQKLLRHFSFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 485

Query: 559  QFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEF 618
            QFFYFDS+ESLP+E +++SDF P NSRYDAQISVFG+K QKKLE+AKVF VGSGALGCEF
Sbjct: 486  QFFYFDSVESLPSEPVDSSDFAPRNSRYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEF 545

Query: 619  LKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHL 678
            LKNLALMGVSC ++GKLT+TDDD+IEKSNLSRQFLFRDWNIGQAKSTVAASAA  IN   
Sbjct: 546  LKNLALMGVSCGSQGKLTVTDDDIIEKSNLSRQFLFRDWNIGQAKSTVAASAAAVINPRF 605

Query: 679  NIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGA 738
            NIEALQNRV  ETENVFDD+FWENL VVVNALDNVNARLYVD RCLYFQKPLLESGTLG 
Sbjct: 606  NIEALQNRVGAETENVFDDAFWENLTVVVNALDNVNARLYVDSRCLYFQKPLLESGTLGT 665

Query: 739  KCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDV 798
            KCNTQ VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +V
Sbjct: 666  KCNTQSVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEV 725

Query: 799  NAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRV 858
            NAYLS+P EYT++MM+AGDAQ+RDTLERI+ECL++E+CETF+DC+TWARL+FEDYF NRV
Sbjct: 726  NAYLSSPVEYTNSMMSAGDAQARDTLERIVECLEKEKCETFQDCLTWARLRFEDYFVNRV 785

Query: 859  KQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPD 918
            KQLIYTFPEDA TS GAPFWSAPKRFP PLQ+S++D S L+F+ A AILRAE++ IPIP+
Sbjct: 786  KQLIYTFPEDAATSTGAPFWSAPKRFPRPLQYSSSDPSLLNFITATAILRAETFGIPIPE 845

Query: 919  WVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRR 978
            W KNP + A+AVDRVIVPDF P++DAKIVTDEKAT+L+TASVDDAAVI DL+ K++  R 
Sbjct: 846  WTKNPKEAAEAVDRVIVPDFEPRQDAKIVTDEKATTLTTASVDDAAVIDDLIAKIDQCRH 905

Query: 979  KLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLI 1038
             L   FRMKPIQFEK                                  DDD+N+HMD+I
Sbjct: 906  NLSPDFRMKPIQFEK----------------------------------DDDTNYHMDVI 965

Query: 1039 AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDY 1098
            AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVE Y
Sbjct: 966  AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEAY 1025

Query: 1099 RNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYS 1158
            RNTFANLALPLFSMAEP+PPKV+KHRDM+WTVWDRW++K NPTLR++++WL++KGL+AYS
Sbjct: 1026 RNTFANLALPLFSMAEPLPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYS 1080

Query: 1159 ISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIP 1218
            ISCGSCLL+NSMF RH++RMDKKVVDLARDVAKVELPPYR HLDVVVACED++DND+DIP
Sbjct: 1086 ISCGSCLLFNSMFTRHKERMDKKVVDLARDVAKVELPPYRNHLDVVVACEDEDDNDVDIP 1080

Query: 1219 LVSVYFR 1222
            LVS+YFR
Sbjct: 1146 LVSIYFR 1080

BLAST of CmoCh20G003570 vs. TAIR 10
Match: AT5G06460.1 (ubiquitin activating enzyme 2 )

HSP 1 Score: 1656.0 bits (4287), Expect = 0.0e+00
Identity = 822/1135 (72.42%), Postives = 932/1135 (82.11%), Query Frame = 0

Query: 95   ASLIKKQRID----SDSNANSKVAAVATGANNIVYDGASLIMASA-----NSNPPDIDED 154
            +S +KK+RID    +D +A +   + + G NN +  G   +M+ A     NSN  +IDED
Sbjct: 15   SSPMKKRRIDHTESADGSAINASNSSSIGLNNSI-GGNDTVMSMAEFGNDNSNNQEIDED 74

Query: 155  LHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVK 214
            LHSRQLAVYGRETMRKLFASNVLISGMQGLG EI                AKN+ILAGVK
Sbjct: 75   LHSRQLAVYGRETMRKLFASNVLISGMQGLGVEI----------------AKNIILAGVK 134

Query: 215  SVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLS 274
            SVTLHDE +VELWDLSSNFVF+E DIGKNRALAS  KLQ+LNN+V V T   KL  EQLS
Sbjct: 135  SVTLHDENVVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLTGKLTKEQLS 194

Query: 275  EFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRG 334
            +F+                       VVF D   +KA E +D+CH+HQPPIAFIK +VRG
Sbjct: 195  DFQ----------------------VVVFVDISFEKATEIDDYCHSHQPPIAFIKADVRG 254

Query: 335  LFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEV 394
            LFGS+FCDFGP FTV DV GE+PH+GIIAS+SN+NP  VSCVDDERLEF+DG+LVVFSEV
Sbjct: 255  LFGSLFCDFGPHFTVLDVDGEEPHSGIIASVSNENPGFVSCVDDERLEFEDGNLVVFSEV 314

Query: 395  HGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAIND 454
             GMTELNDGKPR+IKN + +SFTLEEDT+++G Y KGGIVTQVK+PK+LNFKPLREA+ D
Sbjct: 315  EGMTELNDGKPRKIKNVKPFSFTLEEDTSSYGQYMKGGIVTQVKQPKVLNFKPLREALKD 374

Query: 455  PGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGD 514
            PGDFLLSDFSKFDRPPLLHLAF ALD+F ++ GR P AGSEEDAQKL+ +A ++NE LGD
Sbjct: 375  PGDFLLSDFSKFDRPPLLHLAFQALDRFSSQAGRFPFAGSEEDAQKLVEIAVDINEGLGD 434

Query: 515  GRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESL 574
             R+ED+N KLLRH AFG++AVLNPMAAMFGGIV QEV+KACSGKFHP+ QFFYFDS+ESL
Sbjct: 435  ARLEDVNSKLLRHLAFGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPIFQFFYFDSVESL 494

Query: 575  PTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSC 634
            P E L+AS+FRP NSRYDAQISVFGS LQKKLE+A+VF+VG+GALGCEFLKNLALMGVSC
Sbjct: 495  PKEPLDASEFRPQNSRYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKNLALMGVSC 554

Query: 635  SNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSP 694
              +GKLT+TDDDVIEKSNLSRQFLFRDWNIGQAKSTVAA+AA  IN  LNI+ALQNRV P
Sbjct: 555  GTQGKLTVTDDDVIEKSNLSRQFLFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGP 614

Query: 695  ETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHL 754
            ETENVFDDSFWENL VVVNALDNV ARLYVD RC+YFQKPLLESGTLGAKCNTQMVIPHL
Sbjct: 615  ETENVFDDSFWENLTVVVNALDNVTARLYVDSRCVYFQKPLLESGTLGAKCNTQMVIPHL 674

Query: 755  TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYT 814
            TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +VNAYLS+P EY 
Sbjct: 675  TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSDPVEYM 734

Query: 815  SAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDA 874
             AM  AGDAQ+RDTL R++ECL++E+C +F+DCITWARL+FEDYF+NRVKQL YTFPEDA
Sbjct: 735  KAMRTAGDAQARDTLGRVVECLEKEKCNSFQDCITWARLRFEDYFANRVKQLCYTFPEDA 794

Query: 875  VTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADA 934
             TS GAPFWSAPKRFP PLQFS+ D S+++FV+AA+ILRAE++ IP P+W K    LA+A
Sbjct: 795  ATSTGAPFWSAPKRFPRPLQFSSTDLSHINFVMAASILRAETFGIPTPEWAKTRAGLAEA 854

Query: 935  VDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPI 994
            V+RVIVPDF PKKDA IVTDEKAT+LSTASVDDAAVI +L  KL   R  L   FRMK I
Sbjct: 855  VERVIVPDFEPKKDATIVTDEKATTLSTASVDDAAVIDELNAKLVRCRMSLQPEFRMKAI 914

Query: 995  QFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNY 1054
            QFEK                                  DDD+N+HMD+IAGLANMRARNY
Sbjct: 915  QFEK----------------------------------DDDTNYHMDMIAGLANMRARNY 974

Query: 1055 SIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPL 1114
            S+PEVDKLKAKFIAGRIIPAIATSTAMATG VCLE+YKVLDG HKVEDYRNTFANLALPL
Sbjct: 975  SVPEVDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGSHKVEDYRNTFANLALPL 1034

Query: 1115 FSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNS 1174
            FSMAEPVPPKV+KH+D SWTVWDRW+++ NPTLR+L++WLK KGLNAYSISCGS LLYNS
Sbjct: 1035 FSMAEPVPPKVVKHQDQSWTVWDRWVMRGNPTLRELLDWLKEKGLNAYSISCGSSLLYNS 1076

Query: 1175 MFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1221
            MF RH++RM+++VVDLARDVA VELP YRRH+DVVVACEDD D D+DIPLVSVYF
Sbjct: 1095 MFSRHKERMNRRVVDLARDVAGVELPAYRRHVDVVVACEDDNDADVDIPLVSVYF 1076

BLAST of CmoCh20G003570 vs. TAIR 10
Match: AT2G21470.3 (SUMO-activating enzyme 2 )

HSP 1 Score: 158.3 bits (399), Expect = 3.9e-38
Identity = 156/579 (26.94%), Postives = 246/579 (42.49%), Query Frame = 0

Query: 594  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 653
            Q  ++ AKV MVG+G +GCE LK LAL G        + I D D IE SNL+RQFLFR  
Sbjct: 7    QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66

Query: 654  NIGQAKSTVAASAAVAINKHLNIEALQNRV-SPETENVFDDSFWENLNVVVNALDNVNAR 713
            ++GQ+K+ VA  A +    ++NI +    V +PE    FD  F++  +VV+N LDN++AR
Sbjct: 67   HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126

Query: 714  LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 773
             +V++ CL    PL+ESGT G      + I   TE Y     P  K  P+CT+ S P   
Sbjct: 127  RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186

Query: 774  DHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERC 833
             HC+ WA+   + L  K   D N    N     S    +   ++ D  ER  +       
Sbjct: 187  VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246

Query: 834  ETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQS 893
               ED   + R  ++  F + ++                                     
Sbjct: 247  ---EDIEQYGRKIYDHVFGSNIE------------------------------------- 306

Query: 894  YLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLS 953
                   AA+   E++        KN  +      + ++P+ + +++          S  
Sbjct: 307  -------AALSNEETW--------KNRRRPRPIYSKDVLPESLTQQNG---------STQ 366

Query: 954  TASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRING 1013
              SV D  +   +V+ +     K P+       +   + Q+ L+ +  +K+     +   
Sbjct: 367  NCSVTDGDL---MVSAMPSLGLKNPQ-------ELWGLTQNSLVFIEALKLFFAKRKKVC 426

Query: 1014 VNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAM 1073
            V +  L +   D D    ++ +   AN+RA ++ IP     +AK IAG I+ A+AT+ A+
Sbjct: 427  VEIGHLTF---DKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAI 481

Query: 1074 ATGLVCLELYKVLDGGHKVEDYRNTFA------NLALPLFSMAEPVPPKVIKHRDMSWTV 1133
              GL+ +E  KVL     V+ +R T+        L L      EP P   +     S T 
Sbjct: 487  IAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYV----CSETP 481

Query: 1134 WDRWIIKDNPTLRQLIEWL-KNK-GLNAYSISCGSCLLY 1164
                I      LR L++ + K K G+N   I  G+ LLY
Sbjct: 547  LVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLY 481

BLAST of CmoCh20G003570 vs. TAIR 10
Match: AT2G21470.1 (SUMO-activating enzyme 2 )

HSP 1 Score: 157.1 bits (396), Expect = 8.7e-38
Identity = 156/579 (26.94%), Postives = 245/579 (42.31%), Query Frame = 0

Query: 594  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 653
            Q  ++ AKV MVG+G +GCE LK LAL G        + I D D IE SNL+RQFLFR  
Sbjct: 7    QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66

Query: 654  NIGQAKSTVAASAAVAINKHLNIEALQNRV-SPETENVFDDSFWENLNVVVNALDNVNAR 713
            ++GQ+K+ VA  A +    ++NI +    V +PE    FD  F++  +VV+N LDN++AR
Sbjct: 67   HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126

Query: 714  LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 773
             +V++ CL    PL+ESGT G      + I   TE Y     P  K  P+CT+ S P   
Sbjct: 127  RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186

Query: 774  DHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERC 833
             HC+ WA+   + L  K   D N    N     S    +   ++ D  ER  +       
Sbjct: 187  VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246

Query: 834  ETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQS 893
               ED   + R  ++  F + ++                                     
Sbjct: 247  ---EDIEQYGRKIYDHVFGSNIE------------------------------------- 306

Query: 894  YLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLS 953
                   AA+   E++        KN  +      + ++P+ + +++          S  
Sbjct: 307  -------AALSNEETW--------KNRRRPRPIYSKDVLPESLTQQNG---------STQ 366

Query: 954  TASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRING 1013
              SV D  +   +V+ +     K P+       +   + Q+ L+ +  +K+     +   
Sbjct: 367  NCSVTDGDL---MVSAMPSLGLKNPQ-------ELWGLTQNSLVFIEALKLFFAKRKKEI 426

Query: 1014 VNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAM 1073
             +LT       D D    ++ +   AN+RA ++ IP     +AK IAG I+ A+AT+ A+
Sbjct: 427  GHLTF------DKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAI 478

Query: 1074 ATGLVCLELYKVLDGGHKVEDYRNTFA------NLALPLFSMAEPVPPKVIKHRDMSWTV 1133
              GL+ +E  KVL     V+ +R T+        L L      EP P   +     S T 
Sbjct: 487  IAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYV----CSETP 478

Query: 1134 WDRWIIKDNPTLRQLIEWL-KNK-GLNAYSISCGSCLLY 1164
                I      LR L++ + K K G+N   I  G+ LLY
Sbjct: 547  LVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLY 478

BLAST of CmoCh20G003570 vs. TAIR 10
Match: AT2G21470.2 (SUMO-activating enzyme 2 )

HSP 1 Score: 157.1 bits (396), Expect = 8.7e-38
Identity = 156/579 (26.94%), Postives = 245/579 (42.31%), Query Frame = 0

Query: 594  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 653
            Q  ++ AKV MVG+G +GCE LK LAL G        + I D D IE SNL+RQFLFR  
Sbjct: 7    QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66

Query: 654  NIGQAKSTVAASAAVAINKHLNIEALQNRV-SPETENVFDDSFWENLNVVVNALDNVNAR 713
            ++GQ+K+ VA  A +    ++NI +    V +PE    FD  F++  +VV+N LDN++AR
Sbjct: 67   HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126

Query: 714  LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 773
             +V++ CL    PL+ESGT G      + I   TE Y     P  K  P+CT+ S P   
Sbjct: 127  RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186

Query: 774  DHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERC 833
             HC+ WA+   + L  K   D N    N     S    +   ++ D  ER  +       
Sbjct: 187  VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246

Query: 834  ETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQS 893
               ED   + R  ++  F + ++                                     
Sbjct: 247  ---EDIEQYGRKIYDHVFGSNIE------------------------------------- 306

Query: 894  YLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLS 953
                   AA+   E++        KN  +      + ++P+ + +++          S  
Sbjct: 307  -------AALSNEETW--------KNRRRPRPIYSKDVLPESLTQQNG---------STQ 366

Query: 954  TASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRING 1013
              SV D  +   +V+ +     K P+       +   + Q+ L+ +  +K+     +   
Sbjct: 367  NCSVTDGDL---MVSAMPSLGLKNPQ-------ELWGLTQNSLVFIEALKLFFAKRKKEI 426

Query: 1014 VNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAM 1073
             +LT       D D    ++ +   AN+RA ++ IP     +AK IAG I+ A+AT+ A+
Sbjct: 427  GHLTF------DKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAI 478

Query: 1074 ATGLVCLELYKVLDGGHKVEDYRNTFA------NLALPLFSMAEPVPPKVIKHRDMSWTV 1133
              GL+ +E  KVL     V+ +R T+        L L      EP P   +     S T 
Sbjct: 487  IAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYV----CSETP 478

Query: 1134 WDRWIIKDNPTLRQLIEWL-KNK-GLNAYSISCGSCLLY 1164
                I      LR L++ + K K G+N   I  G+ LLY
Sbjct: 547  LVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLY 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P930280.0e+0072.71Ubiquitin-activating enzyme E1 1 OS=Arabidopsis thaliana OX=3702 GN=UBA1 PE=1 SV... [more]
P929740.0e+0072.42Ubiquitin-activating enzyme E1 2 OS=Arabidopsis thaliana OX=3702 GN=UBA2 PE=1 SV... [more]
P209730.0e+0073.20Ubiquitin-activating enzyme E1 1 OS=Triticum aestivum OX=4565 GN=UBA1 PE=1 SV=1[more]
P312510.0e+0073.11Ubiquitin-activating enzyme E1 2 OS=Triticum aestivum OX=4565 GN=UBA2 PE=2 SV=1[more]
P312520.0e+0069.99Ubiquitin-activating enzyme E1 3 OS=Triticum aestivum OX=4565 GN=UBA3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FYE80.0e+0093.82E1 ubiquitin-activating enzyme OS=Cucurbita moschata OX=3662 GN=LOC111448562 PE=... [more]
A0A6J1JC590.0e+0092.99E1 ubiquitin-activating enzyme OS=Cucurbita maxima OX=3661 GN=LOC111484417 PE=3 ... [more]
A0A5A7VQ320.0e+0087.42E1 ubiquitin-activating enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0KE120.0e+0088.20E1 ubiquitin-activating enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G181590 PE=3 S... [more]
A0A1S3CNI40.0e+0088.78E1 ubiquitin-activating enzyme OS=Cucumis melo OX=3656 GN=LOC103502928 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG7010491.10.0e+0092.58Ubiquitin-activating enzyme E1 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022943999.10.0e+0093.82ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita moschata] >XP_022944... [more]
KAG6570640.10.0e+0092.92Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023512974.10.0e+0093.24ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita pepo subsp. pepo] >X... [more]
XP_022986756.10.0e+0092.99ubiquitin-activating enzyme E1 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G30110.10.0e+0072.71ubiquitin-activating enzyme 1 [more]
AT5G06460.10.0e+0072.42ubiquitin activating enzyme 2 [more]
AT2G21470.33.9e-3826.94SUMO-activating enzyme 2 [more]
AT2G21470.18.7e-3826.94SUMO-activating enzyme 2 [more]
AT2G21470.28.7e-3826.94SUMO-activating enzyme 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000011Ubiquitin/SUMO-activating enzyme E1PRINTSPR01849UBIQUITINACTcoord: 830..857
score: 48.81
coord: 184..208
score: 45.17
coord: 629..652
score: 74.83
coord: 676..701
score: 52.72
IPR018965Ubiquitin-activating enzyme E1, C-terminalSMARTSM00985UBA_e1_C_a_2coord: 1094..1216
e-value: 2.7E-52
score: 189.7
IPR018965Ubiquitin-activating enzyme E1, C-terminalPFAMPF09358E1_UFDcoord: 1127..1216
e-value: 2.2E-22
score: 79.6
IPR042302Ubiquitin-activating enzyme E1, FCCH domain superfamilyGENE3D2.40.30.180coord: 347..428
e-value: 2.6E-111
score: 373.4
IPR019572Ubiquitin-activating enzyme, SCCH domainPFAMPF10585UBA_e1_thiolCyscoord: 769..989
e-value: 1.1E-63
score: 215.6
coord: 1023..1056
e-value: 1.4E-10
score: 41.5
IPR018075Ubiquitin-activating enzyme E1TIGRFAMTIGR01408TIGR01408coord: 141..989
e-value: 0.0
score: 1282.9
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 582..1115
e-value: 2.3E-74
score: 332.9
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 194..564
e-value: 1.6E-18
score: 332.9
IPR032420Ubiquitin-activating enzyme E1, four-helix bundlePFAMPF16191E1_4HBcoord: 431..500
e-value: 9.6E-22
score: 77.0
IPR042449Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1GENE3D3.50.50.80coord: 138..270
e-value: 2.5E-30
score: 107.0
IPR032418Ubiquitin-activating enzyme E1, FCCH domainPFAMPF16190E1_FCCHcoord: 359..429
e-value: 2.1E-27
score: 95.1
NoneNo IPR availableGENE3D3.40.50.12550coord: 304..592
e-value: 2.6E-111
score: 373.4
NoneNo IPR availableGENE3D3.40.50.720coord: 602..1114
e-value: 2.4E-185
score: 618.6
NoneNo IPR availablePIRSRPIRSR039133-1PIRSR039133-1coord: 584..793
e-value: 1.1E-47
score: 160.8
NoneNo IPR availablePIRSRPIRSR039133-2PIRSR039133-2coord: 154..252
e-value: 0.01
score: 12.2
coord: 584..793
e-value: 1.1E-47
score: 160.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..91
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..91
NoneNo IPR availablePANTHERPTHR10953:SF215UBIQUITIN-ACTIVATING ENZYME E1 1coord: 1021..1220
coord: 287..989
NoneNo IPR availablePANTHERPTHR10953:SF215UBIQUITIN-ACTIVATING ENZYME E1 1coord: 124..269
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 287..989
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 124..269
coord: 1021..1220
NoneNo IPR availableCDDcd01490Ube1_repeat2coord: 601..1174
e-value: 0.0
score: 706.746
NoneNo IPR availableCDDcd01491Ube1_repeat1coord: 146..569
e-value: 2.32191E-137
score: 417.054
IPR042063Ubiquitin-activating enzyme E1, SCCH domainGENE3D1.10.10.2660coord: 754..1063
e-value: 2.4E-185
score: 618.6
IPR038252Ubiquitin-activating enzyme E1, C-terminal domain superfamilyGENE3D3.10.290.60coord: 1129..1219
e-value: 4.6E-21
score: 76.9
IPR018074Ubiquitin-activating enzyme E1, conserved sitePROSITEPS00536UBIQUITIN_ACTIVAT_1coord: 544..552
IPR033127Ubiquitin-activating enzyme E1, Cys active sitePROSITEPS00865UBIQUITIN_ACTIVAT_2coord: 761..769
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 577..1111
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 141..566

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G003570.1CmoCh20G003570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006464 cellular protein modification process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004839 ubiquitin activating enzyme activity
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity