Homology
BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match:
P93028 (Ubiquitin-activating enzyme E1 1 OS=Arabidopsis thaliana OX=3702 GN=UBA1 PE=1 SV=1)
HSP 1 Score: 1692.6 bits (4382), Expect = 0.0e+00
Identity = 834/1147 (72.71%), Postives = 956/1147 (83.35%), Query Frame = 0
Query: 79 SSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGASLI----MAS 138
+S N+ N+N++ + KK+RID +++ K +++ ++ + G S++ MA
Sbjct: 6 ASEANDKNDNTIIGSDLASSKKRRIDFTESSSDKSSSILASGSSRGFHGDSVVQQIDMAF 65
Query: 139 ANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLP 198
NSN +IDEDLHSRQLAVYGRETMR+LFASNVLISGM GLGAEI
Sbjct: 66 GNSNRQEIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEI--------------- 125
Query: 199 AAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHT 258
AKN+ILAGVKSVTLHDE +VELWDLSSNFVFSE D+GKNRA AS QKLQDLNN+V+V +
Sbjct: 126 -AKNLILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSS 185
Query: 259 SMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQP 318
L E LS F+ VVF+D +++A+EF+D+CH+HQP
Sbjct: 186 LTKSLNKEDLSGFQ----------------------VVVFSDISMERAIEFDDYCHSHQP 245
Query: 319 PIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEF 378
PIAF+K +VRGLFGSVFCDFGPEF V DV GE+PHTGIIASISN+N A +SCVDDERLEF
Sbjct: 246 PIAFVKADVRGLFGSVFCDFGPEFAVLDVDGEEPHTGIIASISNENQAFISCVDDERLEF 305
Query: 379 QDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKML 438
+DGDLVVFSEV GMTELNDGKPR+IK+ R YSFTL+EDTTN+G+Y KGGIVTQVK+PK+L
Sbjct: 306 EDGDLVVFSEVEGMTELNDGKPRKIKSTRPYSFTLDEDTTNYGTYVKGGIVTQVKQPKLL 365
Query: 439 NFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLIS 498
NFKPLREA+ DPGDFL SDFSKFDRPPLLHLAF ALD F E GR PVAGSEEDAQKLIS
Sbjct: 366 NFKPLREALKDPGDFLFSDFSKFDRPPLLHLAFQALDHFKAEAGRFPVAGSEEDAQKLIS 425
Query: 499 VASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLV 558
+A+ +N GD +VE+++ KLLRHF+FGAKAVLNPMAAMFGGIV QEV+KACSGKFHPL
Sbjct: 426 IATAINTGQGDLKVENVDQKLLRHFSFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 485
Query: 559 QFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEF 618
QFFYFDS+ESLP+E +++SDF P NSRYDAQISVFG+K QKKLE+AKVF VGSGALGCEF
Sbjct: 486 QFFYFDSVESLPSEPVDSSDFAPRNSRYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEF 545
Query: 619 LKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHL 678
LKNLALMGVSC ++GKLT+TDDD+IEKSNLSRQFLFRDWNIGQAKSTVAASAA IN
Sbjct: 546 LKNLALMGVSCGSQGKLTVTDDDIIEKSNLSRQFLFRDWNIGQAKSTVAASAAAVINPRF 605
Query: 679 NIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGA 738
NIEALQNRV ETENVFDD+FWENL VVVNALDNVNARLYVD RCLYFQKPLLESGTLG
Sbjct: 606 NIEALQNRVGAETENVFDDAFWENLTVVVNALDNVNARLYVDSRCLYFQKPLLESGTLGT 665
Query: 739 KCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDV 798
KCNTQ VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +V
Sbjct: 666 KCNTQSVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEV 725
Query: 799 NAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRV 858
NAYLS+P EYT++MM+AGDAQ+RDTLERI+ECL++E+CETF+DC+TWARL+FEDYF NRV
Sbjct: 726 NAYLSSPVEYTNSMMSAGDAQARDTLERIVECLEKEKCETFQDCLTWARLRFEDYFVNRV 785
Query: 859 KQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPD 918
KQLIYTFPEDA TS GAPFWSAPKRFP PLQ+S++D S L+F+ A AILRAE++ IPIP+
Sbjct: 786 KQLIYTFPEDAATSTGAPFWSAPKRFPRPLQYSSSDPSLLNFITATAILRAETFGIPIPE 845
Query: 919 WVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRR 978
W KNP + A+AVDRVIVPDF P++DAKIVTDEKAT+L+TASVDDAAVI DL+ K++ R
Sbjct: 846 WTKNPKEAAEAVDRVIVPDFEPRQDAKIVTDEKATTLTTASVDDAAVIDDLIAKIDQCRH 905
Query: 979 KLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLI 1038
L FRMKPIQFEK DDD+N+HMD+I
Sbjct: 906 NLSPDFRMKPIQFEK----------------------------------DDDTNYHMDVI 965
Query: 1039 AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDY 1098
AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVE Y
Sbjct: 966 AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEAY 1025
Query: 1099 RNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYS 1158
RNTFANLALPLFSMAEP+PPKV+KHRDM+WTVWDRW++K NPTLR++++WL++KGL+AYS
Sbjct: 1026 RNTFANLALPLFSMAEPLPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYS 1080
Query: 1159 ISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIP 1218
ISCGSCLL+NSMF RH++RMDKKVVDLARDVAKVELPPYR HLDVVVACED++DND+DIP
Sbjct: 1086 ISCGSCLLFNSMFTRHKERMDKKVVDLARDVAKVELPPYRNHLDVVVACEDEDDNDVDIP 1080
Query: 1219 LVSVYFR 1222
LVS+YFR
Sbjct: 1146 LVSIYFR 1080
BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match:
P92974 (Ubiquitin-activating enzyme E1 2 OS=Arabidopsis thaliana OX=3702 GN=UBA2 PE=1 SV=1)
HSP 1 Score: 1656.0 bits (4287), Expect = 0.0e+00
Identity = 822/1135 (72.42%), Postives = 932/1135 (82.11%), Query Frame = 0
Query: 95 ASLIKKQRID----SDSNANSKVAAVATGANNIVYDGASLIMASA-----NSNPPDIDED 154
+S +KK+RID +D +A + + + G NN + G +M+ A NSN +IDED
Sbjct: 15 SSPMKKRRIDHTESADGSAINASNSSSIGLNNSI-GGNDTVMSMAEFGNDNSNNQEIDED 74
Query: 155 LHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVK 214
LHSRQLAVYGRETMRKLFASNVLISGMQGLG EI AKN+ILAGVK
Sbjct: 75 LHSRQLAVYGRETMRKLFASNVLISGMQGLGVEI----------------AKNIILAGVK 134
Query: 215 SVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLS 274
SVTLHDE +VELWDLSSNFVF+E DIGKNRALAS KLQ+LNN+V V T KL EQLS
Sbjct: 135 SVTLHDENVVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLTGKLTKEQLS 194
Query: 275 EFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRG 334
+F+ VVF D +KA E +D+CH+HQPPIAFIK +VRG
Sbjct: 195 DFQ----------------------VVVFVDISFEKATEIDDYCHSHQPPIAFIKADVRG 254
Query: 335 LFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEV 394
LFGS+FCDFGP FTV DV GE+PH+GIIAS+SN+NP VSCVDDERLEF+DG+LVVFSEV
Sbjct: 255 LFGSLFCDFGPHFTVLDVDGEEPHSGIIASVSNENPGFVSCVDDERLEFEDGNLVVFSEV 314
Query: 395 HGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAIND 454
GMTELNDGKPR+IKN + +SFTLEEDT+++G Y KGGIVTQVK+PK+LNFKPLREA+ D
Sbjct: 315 EGMTELNDGKPRKIKNVKPFSFTLEEDTSSYGQYMKGGIVTQVKQPKVLNFKPLREALKD 374
Query: 455 PGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGD 514
PGDFLLSDFSKFDRPPLLHLAF ALD+F ++ GR P AGSEEDAQKL+ +A ++NE LGD
Sbjct: 375 PGDFLLSDFSKFDRPPLLHLAFQALDRFSSQAGRFPFAGSEEDAQKLVEIAVDINEGLGD 434
Query: 515 GRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESL 574
R+ED+N KLLRH AFG++AVLNPMAAMFGGIV QEV+KACSGKFHP+ QFFYFDS+ESL
Sbjct: 435 ARLEDVNSKLLRHLAFGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPIFQFFYFDSVESL 494
Query: 575 PTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSC 634
P E L+AS+FRP NSRYDAQISVFGS LQKKLE+A+VF+VG+GALGCEFLKNLALMGVSC
Sbjct: 495 PKEPLDASEFRPQNSRYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKNLALMGVSC 554
Query: 635 SNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSP 694
+GKLT+TDDDVIEKSNLSRQFLFRDWNIGQAKSTVAA+AA IN LNI+ALQNRV P
Sbjct: 555 GTQGKLTVTDDDVIEKSNLSRQFLFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGP 614
Query: 695 ETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHL 754
ETENVFDDSFWENL VVVNALDNV ARLYVD RC+YFQKPLLESGTLGAKCNTQMVIPHL
Sbjct: 615 ETENVFDDSFWENLTVVVNALDNVTARLYVDSRCVYFQKPLLESGTLGAKCNTQMVIPHL 674
Query: 755 TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYT 814
TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +VNAYLS+P EY
Sbjct: 675 TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSDPVEYM 734
Query: 815 SAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDA 874
AM AGDAQ+RDTL R++ECL++E+C +F+DCITWARL+FEDYF+NRVKQL YTFPEDA
Sbjct: 735 KAMRTAGDAQARDTLGRVVECLEKEKCNSFQDCITWARLRFEDYFANRVKQLCYTFPEDA 794
Query: 875 VTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADA 934
TS GAPFWSAPKRFP PLQFS+ D S+++FV+AA+ILRAE++ IP P+W K LA+A
Sbjct: 795 ATSTGAPFWSAPKRFPRPLQFSSTDLSHINFVMAASILRAETFGIPTPEWAKTRAGLAEA 854
Query: 935 VDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPI 994
V+RVIVPDF PKKDA IVTDEKAT+LSTASVDDAAVI +L KL R L FRMK I
Sbjct: 855 VERVIVPDFEPKKDATIVTDEKATTLSTASVDDAAVIDELNAKLVRCRMSLQPEFRMKAI 914
Query: 995 QFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNY 1054
QFEK DDD+N+HMD+IAGLANMRARNY
Sbjct: 915 QFEK----------------------------------DDDTNYHMDMIAGLANMRARNY 974
Query: 1055 SIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPL 1114
S+PEVDKLKAKFIAGRIIPAIATSTAMATG VCLE+YKVLDG HKVEDYRNTFANLALPL
Sbjct: 975 SVPEVDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGSHKVEDYRNTFANLALPL 1034
Query: 1115 FSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNS 1174
FSMAEPVPPKV+KH+D SWTVWDRW+++ NPTLR+L++WLK KGLNAYSISCGS LLYNS
Sbjct: 1035 FSMAEPVPPKVVKHQDQSWTVWDRWVMRGNPTLRELLDWLKEKGLNAYSISCGSSLLYNS 1076
Query: 1175 MFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1221
MF RH++RM+++VVDLARDVA VELP YRRH+DVVVACEDD D D+DIPLVSVYF
Sbjct: 1095 MFSRHKERMNRRVVDLARDVAGVELPAYRRHVDVVVACEDDNDADVDIPLVSVYF 1076
BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match:
P20973 (Ubiquitin-activating enzyme E1 1 OS=Triticum aestivum OX=4565 GN=UBA1 PE=1 SV=1)
HSP 1 Score: 1630.9 bits (4222), Expect = 0.0e+00
Identity = 792/1082 (73.20%), Postives = 910/1082 (84.10%), Query Frame = 0
Query: 141 DIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVI 200
+IDEDLHSRQLAVYGRETM++LF SNVL+SG+QGLGAEI AKN++
Sbjct: 42 EIDEDLHSRQLAVYGRETMKRLFGSNVLVSGLQGLGAEI----------------AKNLV 101
Query: 201 LAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLV 260
LAGVKSVTLHD+G VELWDLSSNF SE+D+G+NRA A QKLQ+LNN+V+V L
Sbjct: 102 LAGVKSVTLHDDGNVELWDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLT 161
Query: 261 MEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIK 320
E LS+F+ AVVFTD LDKA+EF+D+CH+ QPPIAFIK
Sbjct: 162 KEHLSKFQ----------------------AVVFTDISLDKAIEFDDYCHSQQPPIAFIK 221
Query: 321 TEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLV 380
+EVRGLFGSVFCDFGPEFTV DV GE+PHTGI+ASISNDNPALVSCVDDERLEFQDGDLV
Sbjct: 222 SEVRGLFGSVFCDFGPEFTVLDVDGEEPHTGIVASISNDNPALVSCVDDERLEFQDGDLV 281
Query: 381 VFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLR 440
VFSEVHGMTELNDGKPR++KN R YSF LEEDT++FG+Y +GGIVTQVK PK++ FKPL+
Sbjct: 282 VFSEVHGMTELNDGKPRKVKNARPYSFFLEEDTSSFGAYVRGGIVTQVKPPKVIKFKPLK 341
Query: 441 EAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVN 500
EA+++PG+FL+SDFSKF+RPPLLHLAF ALDKF TEL R PVAGS +D Q++I A ++N
Sbjct: 342 EAMSEPGEFLMSDFSKFERPPLLHLAFQALDKFRTELSRFPVAGSTDDVQRVIEYAISIN 401
Query: 501 ESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFD 560
++LGD ++E+I+ KLL HFA G++AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFYFD
Sbjct: 402 DTLGDRKLEEIDKKLLHHFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFD 461
Query: 561 SLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLAL 620
S+ESLP + LE D +P NSRYDAQISVFGSKLQ KLE AK+FMVGSGALGCEFLKNLAL
Sbjct: 462 SVESLPVDPLEPGDLKPKNSRYDAQISVFGSKLQNKLEEAKIFMVGSGALGCEFLKNLAL 521
Query: 621 MGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQ 680
MG+SCS G LT+TDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AA+ IN L++EALQ
Sbjct: 522 MGISCSQNGNLTLTDDDVIEKSNLSRQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQ 581
Query: 681 NRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQM 740
NR SPETENVF+D+FWENL+ VVNALDNV AR+Y+D RC+YFQKPLLESGTLGAKCNTQM
Sbjct: 582 NRASPETENVFNDAFWENLDAVVNALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQM 641
Query: 741 VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSN 800
VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPT+VNA+LSN
Sbjct: 642 VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSN 701
Query: 801 PSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYT 860
P+ Y SA AGDAQ+RD LER++ECLDR++CETF+D ITWARLKFEDYFSNRVKQL +T
Sbjct: 702 PTTYISAARTAGDAQARDQLERVIECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFT 761
Query: 861 FPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPT 920
FPED++TS+GAPFWSAPKRFP P++FS++DQS L F+LAAAILRAE++ IPIP+W K P
Sbjct: 762 FPEDSMTSSGAPFWSAPKRFPRPVEFSSSDQSQLSFILAAAILRAETFGIPIPEWAKTPN 821
Query: 921 KL-ADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEG 980
KL A+AVD+VIVPDF PK+ KIVT EKATSLS+ASVDDAAVI +L+ KLE+ + LP G
Sbjct: 822 KLAAEAVDKVIVPDFQPKQGVKIVTHEKATSLSSASVDDAAVIEELIAKLEEVSKTLPSG 881
Query: 981 FRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLAN 1040
F M PIQFEK DDD+NFHMD+IAG AN
Sbjct: 882 FHMNPIQFEK----------------------------------DDDTNFHMDVIAGFAN 941
Query: 1041 MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA 1100
MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRNTFA
Sbjct: 942 MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRNTFA 1001
Query: 1101 NLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGS 1160
NLA+PLFS+AEPVPPK IKH+++SWTVWDRW + N TLR+L+EWLK KGLNAYSISCG+
Sbjct: 1002 NLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYSISCGT 1051
Query: 1161 CLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVY 1220
LLYNSMFPRH++R+D+KVVD+AR+VAK+E+P YRRHLDVVVACEDD+DND+DIPLVSVY
Sbjct: 1062 SLLYNSMFPRHKERLDRKVVDVAREVAKMEVPSYRRHLDVVVACEDDDDNDVDIPLVSVY 1051
Query: 1221 FR 1222
FR
Sbjct: 1122 FR 1051
BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match:
P31251 (Ubiquitin-activating enzyme E1 2 OS=Triticum aestivum OX=4565 GN=UBA2 PE=2 SV=1)
HSP 1 Score: 1628.2 bits (4215), Expect = 0.0e+00
Identity = 791/1082 (73.11%), Postives = 908/1082 (83.92%), Query Frame = 0
Query: 141 DIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVI 200
+IDEDLHSRQLAVYGRETM+ LF SNVL+SG+QGLGAEI AKN++
Sbjct: 42 EIDEDLHSRQLAVYGRETMKPLFGSNVLVSGLQGLGAEI----------------AKNLV 101
Query: 201 LAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLV 260
LAGVKSVTLHD+G VELWDLSSNF SE+D+G+NRA A QKLQ+LNN+V+V L
Sbjct: 102 LAGVKSVTLHDDGNVELWDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLT 161
Query: 261 MEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIK 320
E LS+F+ AVVFTD LDKA+EF+D+CH+HQPPIAFIK
Sbjct: 162 KEHLSKFQ----------------------AVVFTDISLDKAIEFDDYCHSHQPPIAFIK 221
Query: 321 TEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLV 380
+EVRGLFGSVFCDFGPEFTV DV GE+PHTGI+ASISNDNPALVSCVDDERLEFQDGDLV
Sbjct: 222 SEVRGLFGSVFCDFGPEFTVLDVDGEEPHTGIVASISNDNPALVSCVDDERLEFQDGDLV 281
Query: 381 VFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLR 440
VFSEVHGMTELNDGKPR++KN R YSF LEEDT++FG+Y +GGIVTQVK PK++ FKPL+
Sbjct: 282 VFSEVHGMTELNDGKPRKVKNARPYSFFLEEDTSSFGAYVRGGIVTQVKPPKVIKFKPLK 341
Query: 441 EAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVN 500
EA+++PG+FL+SDFSKF+RPPLLHLAF ALDKF TEL R PVAGS +D Q++I A ++N
Sbjct: 342 EAMSEPGEFLMSDFSKFERPPLLHLAFQALDKFRTELSRFPVAGSTDDVQRVIEYAISIN 401
Query: 501 ESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFD 560
++LGD ++E+I+ KLL HFA G++AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFYFD
Sbjct: 402 DTLGDRKLEEIDKKLLHHFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFD 461
Query: 561 SLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLAL 620
S+ESLP + LE D +P NSRYDAQISVFGS LQ KLE AK+FMVGSGALGCEFLKNLAL
Sbjct: 462 SVESLPVDPLEPGDLKPKNSRYDAQISVFGSTLQNKLEEAKIFMVGSGALGCEFLKNLAL 521
Query: 621 MGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQ 680
MG+SCS G LT+TDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AA+ IN L++EALQ
Sbjct: 522 MGISCSQNGNLTVTDDDVIEKSNLSRQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQ 581
Query: 681 NRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQM 740
NR SPETENVF+D+FWENL+ VVNALDNV AR+Y+D RC+YFQKPLLESGTLGAKCNTQM
Sbjct: 582 NRASPETENVFNDAFWENLDAVVNALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQM 641
Query: 741 VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSN 800
VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPT+VNA+LSN
Sbjct: 642 VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSN 701
Query: 801 PSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYT 860
P+ Y SA AGDAQ+RD LER++ECLDR++CETF+D ITWARLKFEDYFSNRVKQL +T
Sbjct: 702 PTTYISAARTAGDAQARDQLERVIECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFT 761
Query: 861 FPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPT 920
FPED++TS+GAPFWSAPKRFP P++FS++D S L F+LAAAILRAE++ IPI +W K P
Sbjct: 762 FPEDSMTSSGAPFWSAPKRFPRPVEFSSSDPSQLSFILAAAILRAETFGIPISEWAKTPN 821
Query: 921 KL-ADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEG 980
KL A+AVD+VIVPDF PK+ KIVTDEKATSLS+ASVDDAAVI +L+ KLE+ + LP G
Sbjct: 822 KLAAEAVDKVIVPDFQPKQGVKIVTDEKATSLSSASVDDAAVIEELIAKLEEVSKTLPSG 881
Query: 981 FRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLAN 1040
F M PIQFEK DDD+NFHMD+IAG AN
Sbjct: 882 FHMNPIQFEK----------------------------------DDDTNFHMDVIAGFAN 941
Query: 1041 MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA 1100
MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRNTFA
Sbjct: 942 MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRNTFA 1001
Query: 1101 NLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGS 1160
NLA+PLFS+AEPVPPK IKH+++SWTVWDRW + N TLR+L+EWLK KGLNAYSISCG+
Sbjct: 1002 NLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYSISCGT 1051
Query: 1161 CLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVY 1220
LLYNSMFPRH++R+D+KVVD+AR+VAK+E+P YRRHLDVVVACEDD+DND+DIPLVSVY
Sbjct: 1062 SLLYNSMFPRHKERLDRKVVDVAREVAKMEVPSYRRHLDVVVACEDDDDNDVDIPLVSVY 1051
Query: 1221 FR 1222
FR
Sbjct: 1122 FR 1051
BLAST of CmoCh20G003570 vs. ExPASy Swiss-Prot
Match:
P31252 (Ubiquitin-activating enzyme E1 3 OS=Triticum aestivum OX=4565 GN=UBA3 PE=1 SV=1)
HSP 1 Score: 1559.7 bits (4037), Expect = 0.0e+00
Identity = 758/1083 (69.99%), Postives = 886/1083 (81.81%), Query Frame = 0
Query: 139 PPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKN 198
P +IDEDLHSRQLAVYGRETMR+LFAS+VL+SG+ GLGAEI AKN
Sbjct: 43 PQEIDEDLHSRQLAVYGRETMRRLFASDVLVSGLNGLGAEI----------------AKN 102
Query: 199 VILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTK 258
+ LAGVKSVT+HD V++WDLS NF SE DIGKNRA A KLQ+LNN+V++ +
Sbjct: 103 LALAGVKSVTIHDVKTVKMWDLSGNFFLSEDDIGKNRAAACVAKLQELNNAVLISALTEE 162
Query: 259 LVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAF 318
L E LS+F+ AVVFTD LDKA EF+D+CHNHQPPI+F
Sbjct: 163 LTTEHLSKFQ----------------------AVVFTDIDLDKAYEFDDYCHNHQPPISF 222
Query: 319 IKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGD 378
IK+EV GLFGSVFCDFGP+FTV DV GEDPHTGIIASISNDNPAL+SCVDDERLEFQDGD
Sbjct: 223 IKSEVCGLFGSVFCDFGPKFTVLDVDGEDPHTGIIASISNDNPALISCVDDERLEFQDGD 282
Query: 379 LVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKP 438
LVVFSEVHGMTELNDGKPR++KN R +SF++EEDT+NFG Y KGGIVTQVKEPK+L FK
Sbjct: 283 LVVFSEVHGMTELNDGKPRKVKNARPFSFSIEEDTSNFGIYVKGGIVTQVKEPKVLCFKA 342
Query: 439 LREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASN 498
LR+A+ DPG+ LLSDFSKF+RPP+LHLAF ALDKF + GR P AG EEDA + +A+
Sbjct: 343 LRDAMTDPGEVLLSDFSKFERPPVLHLAFQALDKFKKDHGRCPAAGCEEDAHSFLKIAAA 402
Query: 499 VNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFY 558
+NE+ D +++ I+ KL R FA G++AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFY
Sbjct: 403 INEASADRKLDTIDEKLFRQFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLNQFFY 462
Query: 559 FDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNL 618
FDS+ESLPT LE D +P N+RYDAQ+SVFGSKLQKK+E A F+VGSGALGCEFLKNL
Sbjct: 463 FDSVESLPTYPLEPQDLKPSNNRYDAQVSVFGSKLQKKMEEANTFVVGSGALGCEFLKNL 522
Query: 619 ALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEA 678
ALMGVSCS++GKLTITDDD+IEKSNLSRQFLFRDWNIGQAKSTVAA+AA AIN L+I+A
Sbjct: 523 ALMGVSCSSKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAATAASAINPSLHIDA 582
Query: 679 LQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNT 738
LQNR P+TENVF D+FWE L+VV+NALDNVNAR+Y+D RCLYFQKPLLESGTLGAKCN
Sbjct: 583 LQNRACPDTENVFHDTFWEGLDVVINALDNVNARMYMDMRCLYFQKPLLESGTLGAKCNI 642
Query: 739 QMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYL 798
QMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +VN++L
Sbjct: 643 QMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPNEVNSFL 702
Query: 799 SNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLI 858
SNP++Y +AM AGDAQ+R+ LER+ ECL+++RC TF+DCI+WARLKFEDYFSNRVKQL
Sbjct: 703 SNPAQYAAAMRKAGDAQARELLERVSECLNKDRCSTFDDCISWARLKFEDYFSNRVKQLT 762
Query: 859 YTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKN 918
+TFPEDA TS GAPFWSAPKRFP LQFS ADQS+L+F+++A+ILRAES+ + IP+W K+
Sbjct: 763 FTFPEDAATSMGAPFWSAPKRFPRALQFSAADQSHLNFIMSASILRAESFGVAIPEWAKD 822
Query: 919 PTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPE 978
+KLAD V+++ VP F PK+ IVTDEKA++LS+ SVDD AVI DL+ KL++ + L
Sbjct: 823 TSKLADVVNKIAVPTFEPKQGVNIVTDEKASNLSSTSVDDVAVIEDLLAKLQEYAKMLLP 882
Query: 979 GFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLA 1038
GF+MKPIQFEK DDD+NFHMDLI+GLA
Sbjct: 883 GFQMKPIQFEK----------------------------------DDDTNFHMDLISGLA 942
Query: 1039 NMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTF 1098
NMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKV+ G H VEDYRNTF
Sbjct: 943 NMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVIAGEHPVEDYRNTF 1002
Query: 1099 ANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCG 1158
ANLALPLFSMAEPVPPKV+KH++ SWTVWDRW ++ N TL +L++W +KGL AYSISCG
Sbjct: 1003 ANLALPLFSMAEPVPPKVMKHKETSWTVWDRWSVQGNLTLAELLQWFADKGLTAYSISCG 1053
Query: 1159 SCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSV 1218
+ LLYN+MF RH+DR+ KKVVD+AR+VAKV++P YRRHLD+ VACED+++ND+DIPLVSV
Sbjct: 1063 TSLLYNNMFARHKDRLTKKVVDIAREVAKVDVPEYRRHLDIGVACEDEDENDVDIPLVSV 1053
Query: 1219 YFR 1222
YFR
Sbjct: 1123 YFR 1053
BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match:
A0A6J1FYE8 (E1 ubiquitin-activating enzyme OS=Cucurbita moschata OX=3662 GN=LOC111448562 PE=3 SV=1)
HSP 1 Score: 2152.1 bits (5575), Expect = 0.0e+00
Identity = 1093/1165 (93.82%), Postives = 1093/1165 (93.82%), Query Frame = 0
Query: 57 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 116
MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV
Sbjct: 1 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 60
Query: 117 ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 176
ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61 ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120
Query: 177 AEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA 236
AEI AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA
Sbjct: 121 AEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA 180
Query: 237 LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTD 296
LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFE AVVFTD
Sbjct: 181 LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFE----------------------AVVFTD 240
Query: 297 TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 356
TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI
Sbjct: 241 TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 300
Query: 357 SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF 416
SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF
Sbjct: 301 SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF 360
Query: 417 GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE 476
GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE
Sbjct: 361 GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE 420
Query: 477 LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 536
LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG
Sbjct: 421 LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 480
Query: 537 IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK 596
IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK
Sbjct: 481 IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK 540
Query: 597 LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 656
LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG
Sbjct: 541 LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 600
Query: 657 QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD 716
QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD
Sbjct: 601 QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD 660
Query: 717 QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 776
QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL
Sbjct: 661 QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 720
Query: 777 TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE 836
TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE
Sbjct: 721 TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE 780
Query: 837 DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF 896
DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF
Sbjct: 781 DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF 840
Query: 897 VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV 956
VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV
Sbjct: 841 VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV 900
Query: 957 DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLT 1016
DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK
Sbjct: 901 DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK--------------------------- 960
Query: 1017 TLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL 1076
DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL
Sbjct: 961 -------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL 1020
Query: 1077 VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP 1136
VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP
Sbjct: 1021 VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP 1080
Query: 1137 TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH 1196
TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH
Sbjct: 1081 TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH 1093
Query: 1197 LDVVVACEDDEDNDIDIPLVSVYFR 1222
LDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 LDVVVACEDDEDNDIDIPLVSVYFR 1093
BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match:
A0A6J1JC59 (E1 ubiquitin-activating enzyme OS=Cucurbita maxima OX=3661 GN=LOC111484417 PE=3 SV=1)
HSP 1 Score: 2135.1 bits (5531), Expect = 0.0e+00
Identity = 1088/1170 (92.99%), Postives = 1088/1170 (92.99%), Query Frame = 0
Query: 57 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN-----NNSVRNEGASLIKKQRIDSDSNANS 116
MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN NNSVRNEGASLIKKQRIDSDSNANS
Sbjct: 1 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNNNSNNSVRNEGASLIKKQRIDSDSNANS 60
Query: 117 KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 176
KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG
Sbjct: 61 KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 120
Query: 177 MQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI 236
MQGLGAEI AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI
Sbjct: 121 MQGLGAEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI 180
Query: 237 GKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVA 296
GKNRALASAQKLQDLNNSVIVHT TKLV EQLSEFE
Sbjct: 181 GKNRALASAQKLQDLNNSVIVHTLTTKLVKEQLSEFE----------------------V 240
Query: 297 VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG 356
VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG
Sbjct: 241 VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG 300
Query: 357 IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE 416
IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE
Sbjct: 301 IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE 360
Query: 417 DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD 476
DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD
Sbjct: 361 DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD 420
Query: 477 KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA 536
KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA
Sbjct: 421 KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA 480
Query: 537 AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS 596
AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS
Sbjct: 481 AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS 540
Query: 597 KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR 656
KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR
Sbjct: 541 KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR 600
Query: 657 DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA 716
DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA
Sbjct: 601 DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA 660
Query: 717 RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN 776
RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN
Sbjct: 661 RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN 720
Query: 777 IDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRER 836
IDHCLTWARSEFEGLLEKTPTDVNAYL NPSEYTSAMMNAGDAQSRDTLERILECLDRER
Sbjct: 721 IDHCLTWARSEFEGLLEKTPTDVNAYLLNPSEYTSAMMNAGDAQSRDTLERILECLDRER 780
Query: 837 CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ 896
CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ
Sbjct: 781 CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ 840
Query: 897 SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL 956
SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL
Sbjct: 841 SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL 900
Query: 957 STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRIN 1016
STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK
Sbjct: 901 STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK---------------------- 960
Query: 1017 GVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA 1076
DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA
Sbjct: 961 ------------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA 1020
Query: 1077 MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI 1136
MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI
Sbjct: 1021 MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI 1080
Query: 1137 IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP 1196
IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP
Sbjct: 1081 IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP 1098
Query: 1197 PYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
PYRRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 PYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1098
BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match:
A0A5A7VQ32 (E1 ubiquitin-activating enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold615G00120 PE=3 SV=1)
HSP 1 Score: 2108.2 bits (5461), Expect = 0.0e+00
Identity = 1070/1224 (87.42%), Postives = 1110/1224 (90.69%), Query Frame = 0
Query: 1 MWPELGFIGLPPIGDLLVFREVYGGVLDELFLGFVVLLVFFIMLRSFGVFSSLLHFMLPR 60
M P LGF+GL PI LL+F VYG ++ FLGF+V V F++LR FG+FSSLLHFMLPR
Sbjct: 1 MLPSLGFMGLLPIALLLLFHGVYGYDMNGFFLGFLVFFVSFLILRCFGIFSSLLHFMLPR 60
Query: 61 KRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAVA--- 120
KRAGEEGV VEE+TDN S+S NNNN+SV+N GASLIKKQRIDSDSN+N+ VAAVA
Sbjct: 61 KRAGEEGVVVEEETDNGSTSVVFNNNNSSVQNVGASLIKKQRIDSDSNSNTNVAAVATVP 120
Query: 121 TGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGA 180
T ANNIV D ASLIMAS NSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGA
Sbjct: 121 TTANNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGA 180
Query: 181 EIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRAL 240
EI AKNVILAGVKSVTLHDEG+VELWDLSSNFVFSESD+GKNRAL
Sbjct: 181 EI----------------AKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDVGKNRAL 240
Query: 241 ASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDT 300
ASAQKLQDLNNSVIVHT TKLV EQLS+FE VVFTDT
Sbjct: 241 ASAQKLQDLNNSVIVHTLTTKLVKEQLSDFE----------------------VVVFTDT 300
Query: 301 GLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASIS 360
LDKAMEF+DFCHNHQPPI+FIK+EVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASIS
Sbjct: 301 SLDKAMEFDDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASIS 360
Query: 361 NDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFG 420
NDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT NFG
Sbjct: 361 NDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTANFG 420
Query: 421 SYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTEL 480
SYEKGGIVTQVK+PK+LNFKPLREAINDPGDFLLSDFSKFDRPPL+HLAFLALDKFVTEL
Sbjct: 421 SYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTEL 480
Query: 481 GRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGI 540
GRLP+AGSEEDAQKLISVAS +NESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGI
Sbjct: 481 GRLPIAGSEEDAQKLISVASTINESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGI 540
Query: 541 VAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKL 600
VAQEVLKACSGKFHPLVQFFYFDS+ESLPTESL+ASDFRPLNSRYDAQISVFGSKLQKKL
Sbjct: 541 VAQEVLKACSGKFHPLVQFFYFDSVESLPTESLDASDFRPLNSRYDAQISVFGSKLQKKL 600
Query: 601 ENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQ 660
ENAKVFMVGSGALGCEFLKNLALMGVSCS+EGKLTITDDDVIEKSNLSRQFLFRDWNIGQ
Sbjct: 601 ENAKVFMVGSGALGCEFLKNLALMGVSCSSEGKLTITDDDVIEKSNLSRQFLFRDWNIGQ 660
Query: 661 AKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQ 720
AKSTVAA+AAVAIN+HLNIEALQNRVSPETENVFDDSFWENL+VVVNALDNVNARLYVDQ
Sbjct: 661 AKSTVAAAAAVAINRHLNIEALQNRVSPETENVFDDSFWENLSVVVNALDNVNARLYVDQ 720
Query: 721 RCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLT 780
RCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLT
Sbjct: 721 RCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLT 780
Query: 781 WARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFED 840
WARSEFEGLLEKTPTDVNAYLSNP+EYTSAMMNAGDAQSRDTLERILECLDRERCETFED
Sbjct: 781 WARSEFEGLLEKTPTDVNAYLSNPNEYTSAMMNAGDAQSRDTLERILECLDRERCETFED 840
Query: 841 CITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFV 900
CITWARLKFEDYF+NRVKQLIYTFPEDA TSNGAPFWSAPKRFPHPLQFST+DQS+L FV
Sbjct: 841 CITWARLKFEDYFANRVKQLIYTFPEDAQTSNGAPFWSAPKRFPHPLQFSTSDQSHLQFV 900
Query: 901 LAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVD 960
LAAAILRAESYAIP+PDWVKNP KLADA+DRVIVPDFMPKKDAKIVTDEKATSLSTASVD
Sbjct: 901 LAAAILRAESYAIPVPDWVKNPRKLADAIDRVIVPDFMPKKDAKIVTDEKATSLSTASVD 960
Query: 961 DAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTT 1020
DAAVIHDL KLE+TRRKLPEGFRMKPIQFEK
Sbjct: 961 DAAVIHDLAGKLEETRRKLPEGFRMKPIQFEK---------------------------- 1020
Query: 1021 LVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLV 1080
DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLV
Sbjct: 1021 ------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLV 1080
Query: 1081 CLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPT 1140
CLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPT
Sbjct: 1081 CLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPT 1140
Query: 1141 LRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHL 1200
LR+LI+WLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP YRRHL
Sbjct: 1141 LRELIDWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPLYRRHL 1152
Query: 1201 DVVVACEDDEDNDIDIPLVSVYFR 1222
DVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1201 DVVVACEDDEDNDIDIPLVSVYFR 1152
BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match:
A0A0A0KE12 (E1 ubiquitin-activating enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G181590 PE=3 SV=1)
HSP 1 Score: 2060.4 bits (5337), Expect = 0.0e+00
Identity = 1046/1186 (88.20%), Postives = 1081/1186 (91.15%), Query Frame = 0
Query: 41 FIMLRSFGVF--SSLLHFMLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLI 100
F+ SF V +SLLHFMLPRKRAGEEGV VEE+TDN S+S NNNN+SV+N GAS I
Sbjct: 48 FLPSSSFTVLIGTSLLHFMLPRKRAGEEGVVVEEETDNGSTSVVINNNNSSVQNVGASFI 107
Query: 101 KKQRIDSDSNANSKVAAVA---TGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYG 160
KKQRIDSDSN+N+ VAAVA T A+NIV D ASLIMAS NSNPPDIDEDLHSRQLAVYG
Sbjct: 108 KKQRIDSDSNSNTNVAAVATVPTTASNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYG 167
Query: 161 RETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIV 220
RETMRKLFASNVLISGMQGLGAEI AKNVILAGVKSVTLHDEG+V
Sbjct: 168 RETMRKLFASNVLISGMQGLGAEI----------------AKNVILAGVKSVTLHDEGVV 227
Query: 221 ELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIH 280
ELWDLSSNFVFSESD+GKNRALASAQKLQDLNNSVIVHT +KLV EQLS+FE
Sbjct: 228 ELWDLSSNFVFSESDVGKNRALASAQKLQDLNNSVIVHTLTSKLVKEQLSDFE------- 287
Query: 281 GRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFG 340
VVFTDT LDKAMEFNDFCHNHQPPI+FIK+EVRGLFGSVFCDFG
Sbjct: 288 ---------------VVVFTDTSLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFG 347
Query: 341 PEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGK 400
PEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGK
Sbjct: 348 PEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGK 407
Query: 401 PRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFS 460
PRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVK+PK+LNFKPLREAINDPGDFLLSDFS
Sbjct: 408 PRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFS 467
Query: 461 KFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKL 520
KFDRPPL+HLAFLALDKFVTELGRLPVAGSEEDAQKLISVASN+NESLGDGRVEDINPKL
Sbjct: 468 KFDRPPLIHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNINESLGDGRVEDINPKL 527
Query: 521 LRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDF 580
LRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDS+ESLPTESL+AS+F
Sbjct: 528 LRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTESLDASEF 587
Query: 581 RPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITD 640
RPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCS+EGKLTITD
Sbjct: 588 RPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSSEGKLTITD 647
Query: 641 DDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSF 700
DDVIEKSNLSRQFLFRDWNIGQAKSTVAA+AAVAIN+HLNIEALQNRVSPETENVFDDSF
Sbjct: 648 DDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAAVAINRHLNIEALQNRVSPETENVFDDSF 707
Query: 701 WENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDP 760
WENL+VVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDP
Sbjct: 708 WENLSVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDP 767
Query: 761 PEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQ 820
PEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQ
Sbjct: 768 PEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQ 827
Query: 821 SRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWS 880
SRDTLERILECLDRERCETFEDCITWARLKFEDYF+NRVKQLIYTFPEDA TSNGAPFWS
Sbjct: 828 SRDTLERILECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAQTSNGAPFWS 887
Query: 881 APKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFM 940
APKRFPHPLQFST+DQS+L FVLAAAILR+ESYAIPIPDWVKNP KLADA+DR+IVPDFM
Sbjct: 888 APKRFPHPLQFSTSDQSHLQFVLAAAILRSESYAIPIPDWVKNPRKLADAIDRIIVPDFM 947
Query: 941 PKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLL 1000
PKKDAKIVTDEKATSLSTASVDDAAVIHDL KLE+T RKLPEGFRMKPIQFEK
Sbjct: 948 PKKDAKIVTDEKATSLSTASVDDAAVIHDLAGKLEETCRKLPEGFRMKPIQFEK------ 1007
Query: 1001 ILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKA 1060
DDDSNFHMDLIAGLANMRARNYSIPEVDKLKA
Sbjct: 1008 ----------------------------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKA 1067
Query: 1061 KFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPK 1120
KFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPK
Sbjct: 1068 KFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPK 1127
Query: 1121 VIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMD 1180
VIKHRDMSWTVWDRWIIKDNPTLR+LI+WLKNKGLNAYSISCGSCLLYNSMFPRHRDRMD
Sbjct: 1128 VIKHRDMSWTVWDRWIIKDNPTLRELIDWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMD 1161
Query: 1181 KKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
KKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1188 KKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1161
BLAST of CmoCh20G003570 vs. ExPASy TrEMBL
Match:
A0A1S3CNI4 (E1 ubiquitin-activating enzyme OS=Cucumis melo OX=3656 GN=LOC103502928 PE=3 SV=1)
HSP 1 Score: 2050.0 bits (5310), Expect = 0.0e+00
Identity = 1037/1168 (88.78%), Postives = 1069/1168 (91.52%), Query Frame = 0
Query: 57 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 116
MLPRKRAGEEGV VEE+TDN S+S NNNN+SV+N GASLIKKQRIDSDSN+N+ VAAV
Sbjct: 1 MLPRKRAGEEGVVVEEETDNGSTSVVFNNNNSSVQNVGASLIKKQRIDSDSNSNTNVAAV 60
Query: 117 A---TGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 176
A T ANNIV D ASLIMAS NSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ
Sbjct: 61 ATVPTTANNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 120
Query: 177 GLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGK 236
GLGAEI AKNVILAGVKSVTLHDEG+VELWDLSSNFVFSESD+GK
Sbjct: 121 GLGAEI----------------AKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDVGK 180
Query: 237 NRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVV 296
NRALASAQKLQDLNNSVIVHT TKLV EQLS+FE VV
Sbjct: 181 NRALASAQKLQDLNNSVIVHTLTTKLVKEQLSDFE----------------------VVV 240
Query: 297 FTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 356
FTDT LDKAMEF+DFCHNHQPPI+FIK+EVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII
Sbjct: 241 FTDTSLDKAMEFDDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 300
Query: 357 ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT 416
ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT
Sbjct: 301 ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT 360
Query: 417 TNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKF 476
NFGSYEKGGIVTQVK+PK+LNFKPLREAINDPGDFLLSDFSKFDRPPL+HLAFLALDKF
Sbjct: 361 ANFGSYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKF 420
Query: 477 VTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 536
VTELGRLP+AGSEEDAQKLISVAS +NESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM
Sbjct: 421 VTELGRLPIAGSEEDAQKLISVASTINESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 480
Query: 537 FGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKL 596
FGGIVAQEVLKACSGKFHPLVQFFYFDS+ESLPTESL+ASDFRPLNSRYDAQISVFGSKL
Sbjct: 481 FGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTESLDASDFRPLNSRYDAQISVFGSKL 540
Query: 597 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 656
QKKLENAKVFMVGSGALGCEFLKNLALMGVSCS+EGKLTITDDDVIEKSNLSRQFLFRDW
Sbjct: 541 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSSEGKLTITDDDVIEKSNLSRQFLFRDW 600
Query: 657 NIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARL 716
NIGQAKSTVAA+AAVAIN+HLNIEALQNRVSPETENVFDDSFWENL+VVVNALDNVNARL
Sbjct: 601 NIGQAKSTVAAAAAVAINRHLNIEALQNRVSPETENVFDDSFWENLSVVVNALDNVNARL 660
Query: 717 YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 776
YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID
Sbjct: 661 YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 720
Query: 777 HCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCE 836
HCLTWARSEFEGLLEKTPTDVNAYLSNP+EYTSAMMNAGDAQSRDTLERILECLDRERCE
Sbjct: 721 HCLTWARSEFEGLLEKTPTDVNAYLSNPNEYTSAMMNAGDAQSRDTLERILECLDRERCE 780
Query: 837 TFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSY 896
TFEDCITWARLKFEDYF+NRVKQLIYTFPEDA TSNGAPFWSAPKRFPHPLQFST+DQS+
Sbjct: 781 TFEDCITWARLKFEDYFANRVKQLIYTFPEDAQTSNGAPFWSAPKRFPHPLQFSTSDQSH 840
Query: 897 LHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLST 956
L FVLAAAILRAESYAIP+PDWVKNP KLADA+DRVIVPDFMPKKDAKIVTDEKATSLST
Sbjct: 841 LQFVLAAAILRAESYAIPVPDWVKNPRKLADAIDRVIVPDFMPKKDAKIVTDEKATSLST 900
Query: 957 ASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGV 1016
ASVDDAAVIHDL KLE+TRRKLPEGFRMKPIQFEK
Sbjct: 901 ASVDDAAVIHDLAGKLEETRRKLPEGFRMKPIQFEK------------------------ 960
Query: 1017 NLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA 1076
DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA
Sbjct: 961 ----------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA 1020
Query: 1077 TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK 1136
TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK
Sbjct: 1021 TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK 1080
Query: 1137 DNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPY 1196
DNPTLR+LI+WLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP Y
Sbjct: 1081 DNPTLRELIDWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPLY 1096
Query: 1197 RRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
RRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 RRHLDVVVACEDDEDNDIDIPLVSVYFR 1096
BLAST of CmoCh20G003570 vs. NCBI nr
Match:
KAG7010491.1 (Ubiquitin-activating enzyme E1 1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2198.7 bits (5696), Expect = 0.0e+00
Identity = 1123/1213 (92.58%), Postives = 1129/1213 (93.08%), Query Frame = 0
Query: 12 PIGDLLVFREVYGGVLDELFLGFVVLLVFFIMLRSFGVFSSLLHFMLPRKRAGEEGVAVE 71
P + EVYGGVLDELFLGFVVLLVFFIMLRSFGVFSSLLHFMLPRKRAGEEGVAVE
Sbjct: 42 PSSSFTLLIEVYGGVLDELFLGFVVLLVFFIMLRSFGVFSSLLHFMLPRKRAGEEGVAVE 101
Query: 72 EKTDNSSSSN---NNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGA 131
EKTDNSSSS+ NNNN+NNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGA
Sbjct: 102 EKTDNSSSSSNNINNNNSNNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGA 161
Query: 132 SLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLL 191
SLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEI
Sbjct: 162 SLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEI--------- 221
Query: 192 YFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNN 251
AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNN
Sbjct: 222 -------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNN 281
Query: 252 SVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDF 311
SVIVHT T+LV+EQLS+FE AVVFTDTGLDKAMEFNDF
Sbjct: 282 SVIVHTLTTELVIEQLSKFE----------------------AVVFTDTGLDKAMEFNDF 341
Query: 312 CHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVD 371
CHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVD
Sbjct: 342 CHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVD 401
Query: 372 DERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQV 431
DERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQV
Sbjct: 402 DERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQV 461
Query: 432 KEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEED 491
KEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEED
Sbjct: 462 KEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEED 521
Query: 492 AQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSG 551
AQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSG
Sbjct: 522 AQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSG 581
Query: 552 KFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSG 611
KFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSG
Sbjct: 582 KFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSG 641
Query: 612 ALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAV 671
ALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAV
Sbjct: 642 ALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAV 701
Query: 672 AINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLE 731
AINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLE
Sbjct: 702 AINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLE 761
Query: 732 SGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLE 791
SGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLE
Sbjct: 762 SGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLE 821
Query: 792 KTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFED 851
KTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFED
Sbjct: 822 KTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFED 881
Query: 852 YFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESY 911
YFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESY
Sbjct: 882 YFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESY 941
Query: 912 AIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNK 971
AIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNK
Sbjct: 942 AIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNK 1001
Query: 972 LEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSN 1031
LEDTRRKLPEGFRMKPIQFEK DDDSN
Sbjct: 1002 LEDTRRKLPEGFRMKPIQFEK----------------------------------DDDSN 1061
Query: 1032 FHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1091
FHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG
Sbjct: 1062 FHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1121
Query: 1092 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNK 1151
HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNK
Sbjct: 1122 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNK 1181
Query: 1152 GLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDED 1211
GLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDED
Sbjct: 1182 GLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDED 1182
Query: 1212 NDIDIPLVSVYFR 1222
NDIDIPLVSVYFR
Sbjct: 1242 NDIDIPLVSVYFR 1182
BLAST of CmoCh20G003570 vs. NCBI nr
Match:
XP_022943999.1 (ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita moschata] >XP_022944000.1 ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 2152.1 bits (5575), Expect = 0.0e+00
Identity = 1093/1165 (93.82%), Postives = 1093/1165 (93.82%), Query Frame = 0
Query: 57 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 116
MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV
Sbjct: 1 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAV 60
Query: 117 ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 176
ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61 ATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120
Query: 177 AEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA 236
AEI AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA
Sbjct: 121 AEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRA 180
Query: 237 LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTD 296
LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFE AVVFTD
Sbjct: 181 LASAQKLQDLNNSVIVHTSMTKLVMEQLSEFE----------------------AVVFTD 240
Query: 297 TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 356
TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI
Sbjct: 241 TGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 300
Query: 357 SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF 416
SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF
Sbjct: 301 SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNF 360
Query: 417 GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE 476
GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE
Sbjct: 361 GSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTE 420
Query: 477 LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 536
LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG
Sbjct: 421 LGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 480
Query: 537 IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK 596
IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK
Sbjct: 481 IVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKK 540
Query: 597 LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 656
LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG
Sbjct: 541 LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 600
Query: 657 QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD 716
QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD
Sbjct: 601 QAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVD 660
Query: 717 QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 776
QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL
Sbjct: 661 QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 720
Query: 777 TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE 836
TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE
Sbjct: 721 TWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFE 780
Query: 837 DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF 896
DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF
Sbjct: 781 DCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHF 840
Query: 897 VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV 956
VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV
Sbjct: 841 VLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASV 900
Query: 957 DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLT 1016
DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK
Sbjct: 901 DDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK--------------------------- 960
Query: 1017 TLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL 1076
DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL
Sbjct: 961 -------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGL 1020
Query: 1077 VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP 1136
VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP
Sbjct: 1021 VCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNP 1080
Query: 1137 TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH 1196
TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH
Sbjct: 1081 TLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRH 1093
Query: 1197 LDVVVACEDDEDNDIDIPLVSVYFR 1222
LDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 LDVVVACEDDEDNDIDIPLVSVYFR 1093
BLAST of CmoCh20G003570 vs. NCBI nr
Match:
KAG6570640.1 (Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2142.5 bits (5550), Expect = 0.0e+00
Identity = 1090/1173 (92.92%), Postives = 1095/1173 (93.35%), Query Frame = 0
Query: 52 SLLHFMLPRKRAGEEGVAVEEKTDNSSSS---NNNNNNNNSVRNEGASLIKKQRIDSDSN 111
SLLHFMLPRKRAGEEGVAVEEKTDNSSSS NNNNN+NNSVRNEGASLIKKQRIDSDSN
Sbjct: 584 SLLHFMLPRKRAGEEGVAVEEKTDNSSSSSNNNNNNNSNNSVRNEGASLIKKQRIDSDSN 643
Query: 112 ANSKVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVL 171
ANSKVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVL
Sbjct: 644 ANSKVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVL 703
Query: 172 ISGMQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSE 231
ISGMQGLGAEI AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSE
Sbjct: 704 ISGMQGLGAEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSE 763
Query: 232 SDIGKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTT 291
SDIGKNRALASAQKLQDLNNSVIVHT T+LV+EQLS+FE
Sbjct: 764 SDIGKNRALASAQKLQDLNNSVIVHTLTTELVIEQLSKFE-------------------- 823
Query: 292 MVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDP 351
AVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDP
Sbjct: 824 --AVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDP 883
Query: 352 HTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFT 411
HTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFT
Sbjct: 884 HTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFT 943
Query: 412 LEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFL 471
LEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFL
Sbjct: 944 LEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFL 1003
Query: 472 ALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLN 531
ALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLN
Sbjct: 1004 ALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLN 1063
Query: 532 PMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISV 591
PMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISV
Sbjct: 1064 PMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISV 1123
Query: 592 FGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQF 651
FGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQF
Sbjct: 1124 FGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQF 1183
Query: 652 LFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDN 711
LFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDN
Sbjct: 1184 LFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDN 1243
Query: 712 VNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSF 771
VNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSF
Sbjct: 1244 VNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSF 1303
Query: 772 PHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLD 831
PHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLD
Sbjct: 1304 PHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLD 1363
Query: 832 RERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFST 891
RERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFST
Sbjct: 1364 RERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFST 1423
Query: 892 ADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKA 951
ADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKA
Sbjct: 1424 ADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKA 1483
Query: 952 TSLSTASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDL 1011
TSLSTASVDDAA+IHDLVNKLEDT RKLPEGFRMKPIQFEK
Sbjct: 1484 TSLSTASVDDAAIIHDLVNKLEDTSRKLPEGFRMKPIQFEK------------------- 1543
Query: 1012 RINGVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIAT 1071
DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIAT
Sbjct: 1544 ---------------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIAT 1603
Query: 1072 STAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWD 1131
STAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWD
Sbjct: 1604 STAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWD 1663
Query: 1132 RWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKV 1191
RWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKV
Sbjct: 1664 RWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKV 1684
Query: 1192 ELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
ELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1724 ELPPYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1684
BLAST of CmoCh20G003570 vs. NCBI nr
Match:
XP_023512974.1 (ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512976.1 ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2138.2 bits (5539), Expect = 0.0e+00
Identity = 1089/1168 (93.24%), Postives = 1090/1168 (93.32%), Query Frame = 0
Query: 57 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN---NNSVRNEGASLIKKQRIDSDSNANSKV 116
MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN NNSVRNEGASLIKKQRIDSDSNANSKV
Sbjct: 1 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNSNSNNSVRNEGASLIKKQRIDSDSNANSKV 60
Query: 117 AAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 176
AAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ
Sbjct: 61 AAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 120
Query: 177 GLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGK 236
GLGAEI AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGK
Sbjct: 121 GLGAEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGK 180
Query: 237 NRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVV 296
NRALASAQKLQDLNNSVIVHTSMTKLV EQLSEFE VV
Sbjct: 181 NRALASAQKLQDLNNSVIVHTSMTKLVKEQLSEFE----------------------VVV 240
Query: 297 FTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 356
FTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII
Sbjct: 241 FTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 300
Query: 357 ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT 416
ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT
Sbjct: 301 ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDT 360
Query: 417 TNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKF 476
TNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKF
Sbjct: 361 TNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKF 420
Query: 477 VTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 536
VTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM
Sbjct: 421 VTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 480
Query: 537 FGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKL 596
FGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKL
Sbjct: 481 FGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKL 540
Query: 597 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 656
QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW
Sbjct: 541 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 600
Query: 657 NIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARL 716
NIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARL
Sbjct: 601 NIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARL 660
Query: 717 YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 776
YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID
Sbjct: 661 YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 720
Query: 777 HCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCE 836
HCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCE
Sbjct: 721 HCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCE 780
Query: 837 TFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSY 896
TFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSY
Sbjct: 781 TFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSY 840
Query: 897 LHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLST 956
LHFVLAAAILRAESYAI IPDWVKNPTKLADAVDRV+VPDFMPKKDAKIVTDEKATSLST
Sbjct: 841 LHFVLAAAILRAESYAIRIPDWVKNPTKLADAVDRVMVPDFMPKKDAKIVTDEKATSLST 900
Query: 957 ASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGV 1016
ASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK
Sbjct: 901 ASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK------------------------ 960
Query: 1017 NLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA 1076
DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA
Sbjct: 961 ----------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMA 1020
Query: 1077 TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK 1136
TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK
Sbjct: 1021 TGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIK 1080
Query: 1137 DNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPY 1196
DNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPY
Sbjct: 1081 DNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPY 1096
Query: 1197 RRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
RRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 RRHLDVVVACEDDEDNDIDIPLVSVYFR 1096
BLAST of CmoCh20G003570 vs. NCBI nr
Match:
XP_022986756.1 (ubiquitin-activating enzyme E1 1-like [Cucurbita maxima])
HSP 1 Score: 2135.1 bits (5531), Expect = 0.0e+00
Identity = 1088/1170 (92.99%), Postives = 1088/1170 (92.99%), Query Frame = 0
Query: 57 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN-----NNSVRNEGASLIKKQRIDSDSNANS 116
MLPRKRAGEEGVAVEEKTDNSSSSNNNNNN NNSVRNEGASLIKKQRIDSDSNANS
Sbjct: 1 MLPRKRAGEEGVAVEEKTDNSSSSNNNNNNNNNNSNNSVRNEGASLIKKQRIDSDSNANS 60
Query: 117 KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 176
KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG
Sbjct: 61 KVAAVATGANNIVYDGASLIMASANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 120
Query: 177 MQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI 236
MQGLGAEI AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI
Sbjct: 121 MQGLGAEI----------------AKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDI 180
Query: 237 GKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVA 296
GKNRALASAQKLQDLNNSVIVHT TKLV EQLSEFE
Sbjct: 181 GKNRALASAQKLQDLNNSVIVHTLTTKLVKEQLSEFE----------------------V 240
Query: 297 VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG 356
VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG
Sbjct: 241 VVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTG 300
Query: 357 IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE 416
IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE
Sbjct: 301 IIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEE 360
Query: 417 DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD 476
DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD
Sbjct: 361 DTTNFGSYEKGGIVTQVKEPKMLNFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALD 420
Query: 477 KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA 536
KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA
Sbjct: 421 KFVTELGRLPVAGSEEDAQKLISVASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMA 480
Query: 537 AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS 596
AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS
Sbjct: 481 AMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGS 540
Query: 597 KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR 656
KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR
Sbjct: 541 KLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFR 600
Query: 657 DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA 716
DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA
Sbjct: 601 DWNIGQAKSTVAASAAVAINKHLNIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNA 660
Query: 717 RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN 776
RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN
Sbjct: 661 RLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHN 720
Query: 777 IDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRER 836
IDHCLTWARSEFEGLLEKTPTDVNAYL NPSEYTSAMMNAGDAQSRDTLERILECLDRER
Sbjct: 721 IDHCLTWARSEFEGLLEKTPTDVNAYLLNPSEYTSAMMNAGDAQSRDTLERILECLDRER 780
Query: 837 CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ 896
CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ
Sbjct: 781 CETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQ 840
Query: 897 SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL 956
SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL
Sbjct: 841 SYLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSL 900
Query: 957 STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRIN 1016
STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK
Sbjct: 901 STASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEK---------------------- 960
Query: 1017 GVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA 1076
DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA
Sbjct: 961 ------------DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTA 1020
Query: 1077 MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI 1136
MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI
Sbjct: 1021 MATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWI 1080
Query: 1137 IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP 1196
IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP
Sbjct: 1081 IKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELP 1098
Query: 1197 PYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1222
PYRRHLDVVVACEDDEDNDIDIPLVSVYFR
Sbjct: 1141 PYRRHLDVVVACEDDEDNDIDIPLVSVYFR 1098
BLAST of CmoCh20G003570 vs. TAIR 10
Match:
AT2G30110.1 (ubiquitin-activating enzyme 1 )
HSP 1 Score: 1692.6 bits (4382), Expect = 0.0e+00
Identity = 834/1147 (72.71%), Postives = 956/1147 (83.35%), Query Frame = 0
Query: 79 SSNNNNNNNNSVRNEGASLIKKQRIDSDSNANSKVAAVATGANNIVYDGASLI----MAS 138
+S N+ N+N++ + KK+RID +++ K +++ ++ + G S++ MA
Sbjct: 6 ASEANDKNDNTIIGSDLASSKKRRIDFTESSSDKSSSILASGSSRGFHGDSVVQQIDMAF 65
Query: 139 ANSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLP 198
NSN +IDEDLHSRQLAVYGRETMR+LFASNVLISGM GLGAEI
Sbjct: 66 GNSNRQEIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEI--------------- 125
Query: 199 AAKNVILAGVKSVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHT 258
AKN+ILAGVKSVTLHDE +VELWDLSSNFVFSE D+GKNRA AS QKLQDLNN+V+V +
Sbjct: 126 -AKNLILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSS 185
Query: 259 SMTKLVMEQLSEFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQP 318
L E LS F+ VVF+D +++A+EF+D+CH+HQP
Sbjct: 186 LTKSLNKEDLSGFQ----------------------VVVFSDISMERAIEFDDYCHSHQP 245
Query: 319 PIAFIKTEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEF 378
PIAF+K +VRGLFGSVFCDFGPEF V DV GE+PHTGIIASISN+N A +SCVDDERLEF
Sbjct: 246 PIAFVKADVRGLFGSVFCDFGPEFAVLDVDGEEPHTGIIASISNENQAFISCVDDERLEF 305
Query: 379 QDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKML 438
+DGDLVVFSEV GMTELNDGKPR+IK+ R YSFTL+EDTTN+G+Y KGGIVTQVK+PK+L
Sbjct: 306 EDGDLVVFSEVEGMTELNDGKPRKIKSTRPYSFTLDEDTTNYGTYVKGGIVTQVKQPKLL 365
Query: 439 NFKPLREAINDPGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLIS 498
NFKPLREA+ DPGDFL SDFSKFDRPPLLHLAF ALD F E GR PVAGSEEDAQKLIS
Sbjct: 366 NFKPLREALKDPGDFLFSDFSKFDRPPLLHLAFQALDHFKAEAGRFPVAGSEEDAQKLIS 425
Query: 499 VASNVNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLV 558
+A+ +N GD +VE+++ KLLRHF+FGAKAVLNPMAAMFGGIV QEV+KACSGKFHPL
Sbjct: 426 IATAINTGQGDLKVENVDQKLLRHFSFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 485
Query: 559 QFFYFDSLESLPTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEF 618
QFFYFDS+ESLP+E +++SDF P NSRYDAQISVFG+K QKKLE+AKVF VGSGALGCEF
Sbjct: 486 QFFYFDSVESLPSEPVDSSDFAPRNSRYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEF 545
Query: 619 LKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHL 678
LKNLALMGVSC ++GKLT+TDDD+IEKSNLSRQFLFRDWNIGQAKSTVAASAA IN
Sbjct: 546 LKNLALMGVSCGSQGKLTVTDDDIIEKSNLSRQFLFRDWNIGQAKSTVAASAAAVINPRF 605
Query: 679 NIEALQNRVSPETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGA 738
NIEALQNRV ETENVFDD+FWENL VVVNALDNVNARLYVD RCLYFQKPLLESGTLG
Sbjct: 606 NIEALQNRVGAETENVFDDAFWENLTVVVNALDNVNARLYVDSRCLYFQKPLLESGTLGT 665
Query: 739 KCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDV 798
KCNTQ VIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +V
Sbjct: 666 KCNTQSVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEV 725
Query: 799 NAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRV 858
NAYLS+P EYT++MM+AGDAQ+RDTLERI+ECL++E+CETF+DC+TWARL+FEDYF NRV
Sbjct: 726 NAYLSSPVEYTNSMMSAGDAQARDTLERIVECLEKEKCETFQDCLTWARLRFEDYFVNRV 785
Query: 859 KQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPD 918
KQLIYTFPEDA TS GAPFWSAPKRFP PLQ+S++D S L+F+ A AILRAE++ IPIP+
Sbjct: 786 KQLIYTFPEDAATSTGAPFWSAPKRFPRPLQYSSSDPSLLNFITATAILRAETFGIPIPE 845
Query: 919 WVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRR 978
W KNP + A+AVDRVIVPDF P++DAKIVTDEKAT+L+TASVDDAAVI DL+ K++ R
Sbjct: 846 WTKNPKEAAEAVDRVIVPDFEPRQDAKIVTDEKATTLTTASVDDAAVIDDLIAKIDQCRH 905
Query: 979 KLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLI 1038
L FRMKPIQFEK DDD+N+HMD+I
Sbjct: 906 NLSPDFRMKPIQFEK----------------------------------DDDTNYHMDVI 965
Query: 1039 AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDY 1098
AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVE Y
Sbjct: 966 AGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEAY 1025
Query: 1099 RNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYS 1158
RNTFANLALPLFSMAEP+PPKV+KHRDM+WTVWDRW++K NPTLR++++WL++KGL+AYS
Sbjct: 1026 RNTFANLALPLFSMAEPLPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYS 1080
Query: 1159 ISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIP 1218
ISCGSCLL+NSMF RH++RMDKKVVDLARDVAKVELPPYR HLDVVVACED++DND+DIP
Sbjct: 1086 ISCGSCLLFNSMFTRHKERMDKKVVDLARDVAKVELPPYRNHLDVVVACEDEDDNDVDIP 1080
Query: 1219 LVSVYFR 1222
LVS+YFR
Sbjct: 1146 LVSIYFR 1080
BLAST of CmoCh20G003570 vs. TAIR 10
Match:
AT5G06460.1 (ubiquitin activating enzyme 2 )
HSP 1 Score: 1656.0 bits (4287), Expect = 0.0e+00
Identity = 822/1135 (72.42%), Postives = 932/1135 (82.11%), Query Frame = 0
Query: 95 ASLIKKQRID----SDSNANSKVAAVATGANNIVYDGASLIMASA-----NSNPPDIDED 154
+S +KK+RID +D +A + + + G NN + G +M+ A NSN +IDED
Sbjct: 15 SSPMKKRRIDHTESADGSAINASNSSSIGLNNSI-GGNDTVMSMAEFGNDNSNNQEIDED 74
Query: 155 LHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIVFYFNDDLLYFASLPAAKNVILAGVK 214
LHSRQLAVYGRETMRKLFASNVLISGMQGLG EI AKN+ILAGVK
Sbjct: 75 LHSRQLAVYGRETMRKLFASNVLISGMQGLGVEI----------------AKNIILAGVK 134
Query: 215 SVTLHDEGIVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTSMTKLVMEQLS 274
SVTLHDE +VELWDLSSNFVF+E DIGKNRALAS KLQ+LNN+V V T KL EQLS
Sbjct: 135 SVTLHDENVVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLTGKLTKEQLS 194
Query: 275 EFEPIEHKIHGRSSPRLPFEMTTMVAVVFTDTGLDKAMEFNDFCHNHQPPIAFIKTEVRG 334
+F+ VVF D +KA E +D+CH+HQPPIAFIK +VRG
Sbjct: 195 DFQ----------------------VVVFVDISFEKATEIDDYCHSHQPPIAFIKADVRG 254
Query: 335 LFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEV 394
LFGS+FCDFGP FTV DV GE+PH+GIIAS+SN+NP VSCVDDERLEF+DG+LVVFSEV
Sbjct: 255 LFGSLFCDFGPHFTVLDVDGEEPHSGIIASVSNENPGFVSCVDDERLEFEDGNLVVFSEV 314
Query: 395 HGMTELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKEPKMLNFKPLREAIND 454
GMTELNDGKPR+IKN + +SFTLEEDT+++G Y KGGIVTQVK+PK+LNFKPLREA+ D
Sbjct: 315 EGMTELNDGKPRKIKNVKPFSFTLEEDTSSYGQYMKGGIVTQVKQPKVLNFKPLREALKD 374
Query: 455 PGDFLLSDFSKFDRPPLLHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNVNESLGD 514
PGDFLLSDFSKFDRPPLLHLAF ALD+F ++ GR P AGSEEDAQKL+ +A ++NE LGD
Sbjct: 375 PGDFLLSDFSKFDRPPLLHLAFQALDRFSSQAGRFPFAGSEEDAQKLVEIAVDINEGLGD 434
Query: 515 GRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSLESL 574
R+ED+N KLLRH AFG++AVLNPMAAMFGGIV QEV+KACSGKFHP+ QFFYFDS+ESL
Sbjct: 435 ARLEDVNSKLLRHLAFGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPIFQFFYFDSVESL 494
Query: 575 PTESLEASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSC 634
P E L+AS+FRP NSRYDAQISVFGS LQKKLE+A+VF+VG+GALGCEFLKNLALMGVSC
Sbjct: 495 PKEPLDASEFRPQNSRYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKNLALMGVSC 554
Query: 635 SNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAVAINKHLNIEALQNRVSP 694
+GKLT+TDDDVIEKSNLSRQFLFRDWNIGQAKSTVAA+AA IN LNI+ALQNRV P
Sbjct: 555 GTQGKLTVTDDDVIEKSNLSRQFLFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGP 614
Query: 695 ETENVFDDSFWENLNVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHL 754
ETENVFDDSFWENL VVVNALDNV ARLYVD RC+YFQKPLLESGTLGAKCNTQMVIPHL
Sbjct: 615 ETENVFDDSFWENLTVVVNALDNVTARLYVDSRCVYFQKPLLESGTLGAKCNTQMVIPHL 674
Query: 755 TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYT 814
TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP +VNAYLS+P EY
Sbjct: 675 TENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSDPVEYM 734
Query: 815 SAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFSNRVKQLIYTFPEDA 874
AM AGDAQ+RDTL R++ECL++E+C +F+DCITWARL+FEDYF+NRVKQL YTFPEDA
Sbjct: 735 KAMRTAGDAQARDTLGRVVECLEKEKCNSFQDCITWARLRFEDYFANRVKQLCYTFPEDA 794
Query: 875 VTSNGAPFWSAPKRFPHPLQFSTADQSYLHFVLAAAILRAESYAIPIPDWVKNPTKLADA 934
TS GAPFWSAPKRFP PLQFS+ D S+++FV+AA+ILRAE++ IP P+W K LA+A
Sbjct: 795 ATSTGAPFWSAPKRFPRPLQFSSTDLSHINFVMAASILRAETFGIPTPEWAKTRAGLAEA 854
Query: 935 VDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPI 994
V+RVIVPDF PKKDA IVTDEKAT+LSTASVDDAAVI +L KL R L FRMK I
Sbjct: 855 VERVIVPDFEPKKDATIVTDEKATTLSTASVDDAAVIDELNAKLVRCRMSLQPEFRMKAI 914
Query: 995 QFEKVVQDLLILLCVIKIISGDLRINGVNLTTLVWMVQDDDSNFHMDLIAGLANMRARNY 1054
QFEK DDD+N+HMD+IAGLANMRARNY
Sbjct: 915 QFEK----------------------------------DDDTNYHMDMIAGLANMRARNY 974
Query: 1055 SIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPL 1114
S+PEVDKLKAKFIAGRIIPAIATSTAMATG VCLE+YKVLDG HKVEDYRNTFANLALPL
Sbjct: 975 SVPEVDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGSHKVEDYRNTFANLALPL 1034
Query: 1115 FSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRQLIEWLKNKGLNAYSISCGSCLLYNS 1174
FSMAEPVPPKV+KH+D SWTVWDRW+++ NPTLR+L++WLK KGLNAYSISCGS LLYNS
Sbjct: 1035 FSMAEPVPPKVVKHQDQSWTVWDRWVMRGNPTLRELLDWLKEKGLNAYSISCGSSLLYNS 1076
Query: 1175 MFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1221
MF RH++RM+++VVDLARDVA VELP YRRH+DVVVACEDD D D+DIPLVSVYF
Sbjct: 1095 MFSRHKERMNRRVVDLARDVAGVELPAYRRHVDVVVACEDDNDADVDIPLVSVYF 1076
BLAST of CmoCh20G003570 vs. TAIR 10
Match:
AT2G21470.3 (SUMO-activating enzyme 2 )
HSP 1 Score: 158.3 bits (399), Expect = 3.9e-38
Identity = 156/579 (26.94%), Postives = 246/579 (42.49%), Query Frame = 0
Query: 594 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 653
Q ++ AKV MVG+G +GCE LK LAL G + I D D IE SNL+RQFLFR
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66
Query: 654 NIGQAKSTVAASAAVAINKHLNIEALQNRV-SPETENVFDDSFWENLNVVVNALDNVNAR 713
++GQ+K+ VA A + ++NI + V +PE FD F++ +VV+N LDN++AR
Sbjct: 67 HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126
Query: 714 LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 773
+V++ CL PL+ESGT G + I TE Y P K P+CT+ S P
Sbjct: 127 RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186
Query: 774 DHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERC 833
HC+ WA+ + L K D N N S + ++ D ER +
Sbjct: 187 VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246
Query: 834 ETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQS 893
ED + R ++ F + ++
Sbjct: 247 ---EDIEQYGRKIYDHVFGSNIE------------------------------------- 306
Query: 894 YLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLS 953
AA+ E++ KN + + ++P+ + +++ S
Sbjct: 307 -------AALSNEETW--------KNRRRPRPIYSKDVLPESLTQQNG---------STQ 366
Query: 954 TASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRING 1013
SV D + +V+ + K P+ + + Q+ L+ + +K+ +
Sbjct: 367 NCSVTDGDL---MVSAMPSLGLKNPQ-------ELWGLTQNSLVFIEALKLFFAKRKKVC 426
Query: 1014 VNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAM 1073
V + L + D D ++ + AN+RA ++ IP +AK IAG I+ A+AT+ A+
Sbjct: 427 VEIGHLTF---DKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAI 481
Query: 1074 ATGLVCLELYKVLDGGHKVEDYRNTFA------NLALPLFSMAEPVPPKVIKHRDMSWTV 1133
GL+ +E KVL V+ +R T+ L L EP P + S T
Sbjct: 487 IAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYV----CSETP 481
Query: 1134 WDRWIIKDNPTLRQLIEWL-KNK-GLNAYSISCGSCLLY 1164
I LR L++ + K K G+N I G+ LLY
Sbjct: 547 LVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLY 481
BLAST of CmoCh20G003570 vs. TAIR 10
Match:
AT2G21470.1 (SUMO-activating enzyme 2 )
HSP 1 Score: 157.1 bits (396), Expect = 8.7e-38
Identity = 156/579 (26.94%), Postives = 245/579 (42.31%), Query Frame = 0
Query: 594 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 653
Q ++ AKV MVG+G +GCE LK LAL G + I D D IE SNL+RQFLFR
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66
Query: 654 NIGQAKSTVAASAAVAINKHLNIEALQNRV-SPETENVFDDSFWENLNVVVNALDNVNAR 713
++GQ+K+ VA A + ++NI + V +PE FD F++ +VV+N LDN++AR
Sbjct: 67 HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126
Query: 714 LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 773
+V++ CL PL+ESGT G + I TE Y P K P+CT+ S P
Sbjct: 127 RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186
Query: 774 DHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERC 833
HC+ WA+ + L K D N N S + ++ D ER +
Sbjct: 187 VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246
Query: 834 ETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQS 893
ED + R ++ F + ++
Sbjct: 247 ---EDIEQYGRKIYDHVFGSNIE------------------------------------- 306
Query: 894 YLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLS 953
AA+ E++ KN + + ++P+ + +++ S
Sbjct: 307 -------AALSNEETW--------KNRRRPRPIYSKDVLPESLTQQNG---------STQ 366
Query: 954 TASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRING 1013
SV D + +V+ + K P+ + + Q+ L+ + +K+ +
Sbjct: 367 NCSVTDGDL---MVSAMPSLGLKNPQ-------ELWGLTQNSLVFIEALKLFFAKRKKEI 426
Query: 1014 VNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAM 1073
+LT D D ++ + AN+RA ++ IP +AK IAG I+ A+AT+ A+
Sbjct: 427 GHLTF------DKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAI 478
Query: 1074 ATGLVCLELYKVLDGGHKVEDYRNTFA------NLALPLFSMAEPVPPKVIKHRDMSWTV 1133
GL+ +E KVL V+ +R T+ L L EP P + S T
Sbjct: 487 IAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYV----CSETP 478
Query: 1134 WDRWIIKDNPTLRQLIEWL-KNK-GLNAYSISCGSCLLY 1164
I LR L++ + K K G+N I G+ LLY
Sbjct: 547 LVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLY 478
BLAST of CmoCh20G003570 vs. TAIR 10
Match:
AT2G21470.2 (SUMO-activating enzyme 2 )
HSP 1 Score: 157.1 bits (396), Expect = 8.7e-38
Identity = 156/579 (26.94%), Postives = 245/579 (42.31%), Query Frame = 0
Query: 594 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 653
Q ++ AKV MVG+G +GCE LK LAL G + I D D IE SNL+RQFLFR
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66
Query: 654 NIGQAKSTVAASAAVAINKHLNIEALQNRV-SPETENVFDDSFWENLNVVVNALDNVNAR 713
++GQ+K+ VA A + ++NI + V +PE FD F++ +VV+N LDN++AR
Sbjct: 67 HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126
Query: 714 LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 773
+V++ CL PL+ESGT G + I TE Y P K P+CT+ S P
Sbjct: 127 RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186
Query: 774 DHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMMNAGDAQSRDTLERILECLDRERC 833
HC+ WA+ + L K D N N S + ++ D ER +
Sbjct: 187 VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246
Query: 834 ETFEDCITWARLKFEDYFSNRVKQLIYTFPEDAVTSNGAPFWSAPKRFPHPLQFSTADQS 893
ED + R ++ F + ++
Sbjct: 247 ---EDIEQYGRKIYDHVFGSNIE------------------------------------- 306
Query: 894 YLHFVLAAAILRAESYAIPIPDWVKNPTKLADAVDRVIVPDFMPKKDAKIVTDEKATSLS 953
AA+ E++ KN + + ++P+ + +++ S
Sbjct: 307 -------AALSNEETW--------KNRRRPRPIYSKDVLPESLTQQNG---------STQ 366
Query: 954 TASVDDAAVIHDLVNKLEDTRRKLPEGFRMKPIQFEKVVQDLLILLCVIKIISGDLRING 1013
SV D + +V+ + K P+ + + Q+ L+ + +K+ +
Sbjct: 367 NCSVTDGDL---MVSAMPSLGLKNPQ-------ELWGLTQNSLVFIEALKLFFAKRKKEI 426
Query: 1014 VNLTTLVWMVQDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAM 1073
+LT D D ++ + AN+RA ++ IP +AK IAG I+ A+AT+ A+
Sbjct: 427 GHLTF------DKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAI 478
Query: 1074 ATGLVCLELYKVLDGGHKVEDYRNTFA------NLALPLFSMAEPVPPKVIKHRDMSWTV 1133
GL+ +E KVL V+ +R T+ L L EP P + S T
Sbjct: 487 IAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYV----CSETP 478
Query: 1134 WDRWIIKDNPTLRQLIEWL-KNK-GLNAYSISCGSCLLY 1164
I LR L++ + K K G+N I G+ LLY
Sbjct: 547 LVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLY 478
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
P93028 | 0.0e+00 | 72.71 | Ubiquitin-activating enzyme E1 1 OS=Arabidopsis thaliana OX=3702 GN=UBA1 PE=1 SV... | [more] |
P92974 | 0.0e+00 | 72.42 | Ubiquitin-activating enzyme E1 2 OS=Arabidopsis thaliana OX=3702 GN=UBA2 PE=1 SV... | [more] |
P20973 | 0.0e+00 | 73.20 | Ubiquitin-activating enzyme E1 1 OS=Triticum aestivum OX=4565 GN=UBA1 PE=1 SV=1 | [more] |
P31251 | 0.0e+00 | 73.11 | Ubiquitin-activating enzyme E1 2 OS=Triticum aestivum OX=4565 GN=UBA2 PE=2 SV=1 | [more] |
P31252 | 0.0e+00 | 69.99 | Ubiquitin-activating enzyme E1 3 OS=Triticum aestivum OX=4565 GN=UBA3 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FYE8 | 0.0e+00 | 93.82 | E1 ubiquitin-activating enzyme OS=Cucurbita moschata OX=3662 GN=LOC111448562 PE=... | [more] |
A0A6J1JC59 | 0.0e+00 | 92.99 | E1 ubiquitin-activating enzyme OS=Cucurbita maxima OX=3661 GN=LOC111484417 PE=3 ... | [more] |
A0A5A7VQ32 | 0.0e+00 | 87.42 | E1 ubiquitin-activating enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |
A0A0A0KE12 | 0.0e+00 | 88.20 | E1 ubiquitin-activating enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G181590 PE=3 S... | [more] |
A0A1S3CNI4 | 0.0e+00 | 88.78 | E1 ubiquitin-activating enzyme OS=Cucumis melo OX=3656 GN=LOC103502928 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
KAG7010491.1 | 0.0e+00 | 92.58 | Ubiquitin-activating enzyme E1 1 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022943999.1 | 0.0e+00 | 93.82 | ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita moschata] >XP_022944... | [more] |
KAG6570640.1 | 0.0e+00 | 92.92 | Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_023512974.1 | 0.0e+00 | 93.24 | ubiquitin-activating enzyme E1 1-like isoform X1 [Cucurbita pepo subsp. pepo] >X... | [more] |
XP_022986756.1 | 0.0e+00 | 92.99 | ubiquitin-activating enzyme E1 1-like [Cucurbita maxima] | [more] |