CmoCh20G003220 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G003220
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionCalcium uniporter protein
LocationCmo_Chr20: 1573197 .. 1576568 (+)
RNA-Seq ExpressionCmoCh20G003220
SyntenyCmoCh20G003220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGTGGAGGAGATGGTGTTCTAGTGCTTGTTTATTGAGTCGGAGATTGCTGGTGAATGGAGTTCGCAGGCCTTCTTCTCCGGTATCTTCTTATGGAGATTTCGGACTTGGAGGAGTTTCTTTGATTCCATCGACGGCGGTACGGTCGTCGGCCTGGTTTTGTAACGGAGTGGTTTCGTTTTCGTCATCTGTGGATTCGGAAAAATCTGCAGGAGTTGGTAGCGATGGGGGAAAACGTGCGAGCGGTGGTCCTGGGCTTGGGCTTGGGCTTGGCTCTGTGAATGAAGAATCTGGTAATGAAATTAGTGGAGAGACGATATCGTTTGCGGAAGCCAAAAGGCTGATGAGATTGGTAAATGTTGAGGCGCTGAAGATGAAATTGGGGACTGATGGGAAGGAGGCCATTGGATACTCGGAGCTTATCGAAGCTTGCAAAAGCATGGGCATCGCTAGGTCTCAGGACGAAGCTGCTGCGTTTGCTCGAGTCCTCGATGAGGCTGGTGTGGTTCTTCTGTTTCGCGATAAGGTCTATCTTCATCCTGACAAGGTTCGTCAAATCGGTGGGACCTTATTCAATTAATGCACTGCATTGCATCATGTGGCGTGGCTGTTTTATCTATTCATAAATCATCTCATTCAATTTTTGGTTAATTTAGAGTCAGAAATACGGGTGGTTGGTAGAAATTTTCCTAAAAGGGTCAAGACTTTTATATAATGGTTTGATTTAGATGTATAGGTTTTTCAATTTTCTAAACAGTTAAGGAACAGAGTAGGGAAAATTTTGAAGTTTGAATATATTTCTTCTTTTGAATCTAAGACGAATCAAGGGTGGGTGAGCTCTAACAAAGAGCTTAGAGATAATAAGTTAGATCACTGGTGGGTCTAAGAATTTTCTTGTCGCCCAAATATTGTAGGGTCATGTGGGTTCCCTCATGAGATCAACTGTTGAGTGCAAGTTGGGTCCGCTAGGAACTACGACTCTCACAATGATAATCATTGTCTACTTTGAGAATAAGCTCTCATATTTCTCCGTTTACATAAGATATCTCATAGATTTATTTCTGTACTTATAAACCCATGAACAATCCTTTAATTAGCGGACCCCGGACTTGTTTTTCAACAATCCTTTAACACATTCATGGATAGATATTGAAAGATGAAAGATAAATCAAAAGGGACATGCTTGGAAGTTCTGATTTGTCTGTATGGTTTTTGGCTTTTGCGTTTGTTTTTTTCGCTGAAAAAGAACATGTCATTCGGCCAATAAGGTTTGGGGGTCCAGCTAGGTCGGTAGGAGCTGGCGTTTAGAAGTCTTTGAACGGATTTTCCTCCCTTTATGGAGAAAAAACCTAGCTTTTCATTAAGATGAAGGAAGGTCAAATCAAAAGGCAAGGTCGACCAAAAGGTGAAACGCGCAGAAAACACGGCTCCGGGAAGGTAACCAGTTGTTGGTTACCAAAATGGGCAGTTTTCAGAATTTGCACGTAAACATTGAAAAGGTAGAGAAGAAAAGCAATGCTGATTAAGAGATTACCGTAGTGGGAAGGGAAGTTTTTGAGGACTTGCGATTCTCAACTACGTCCCCTTAAACTGCCAAGAGCCAATAGTTTGGGCCAGGATTTTATTTGTTTCTTTATTTAGTTTTGGAGAACGAGCCAAACTTCCCATTTGATTACTTGAAAGTTTCATATACTTTAGAAGAATACCTACAAAAAGGCTCCTAAATTGGTGTTTAAGTTTACAAGTAAAAGGAAAGAACACCAAGTGGAACATAGCAATCTAGGAAAGTCAAAAGTTTCGTCCCATATTTTCCAAGACGGGAGCGGTTCACCTCATTTAACCCATTACTTTTATGCTCTCCCTTTGAATCTATGTCCACACAAAACTTTAGAAATGTTGTCTTTTGCTTCCCTGTCATGAACTTGGGATATATGACACTGTCTGTAACATCAAGGACGTCTAAAATTCCTTGCAAATGGTCGGTGAAAATGTACATGACAAACATCTTTCATTGCTTCTGTACTCAGTATAACATATTTAGTTGAACTAGAATTGATCAGAGCTGCCTCTCTTGTTTTACTTGTTTTTAATCTATAACGTGAGCGTTCCAGTCTTGCCTTCCAGTTTTCTGGGTTTTGAATTCTTAGAAACTTGATTAGCAGATTTTAAGTGTTGTATTTCTTTGTTCTGAGGATCTGATTCCGATGTCAAAACTGCAAGTGTGCCTAATCCTCAGCCTTTTTCTGGGATTTTGAGTTTTCTCAGAAGTGTTCTTCCTGTAAGAACCTAGCTTTACCAAACCATATCTATCTGATTCGGGTTAAACGATCTGTTAGTTCTCGAAACCGGTATCTCTTTTGATGAAGAAATACTGTGGATTTGAGTGAGCATCAATCATTTTATCTGAGTAGTCATTATATTCACATTCTTATTAGTGCATGCTGGAAGCTTCAATCTTGTTGCTCAGTAGGTCATATCCTGGATGCTTGTTTAGCATTTTGTTGTCACATCTTCTTTTCATGAGATCCCACGTTAATTGGAGAGAGGAAAACGAAGCATTGCATAGTAGGGTGTGATAACCTCTCTCTAGCAGACGTGTTTTAAACCCTTAAGGGGAAGCCTGGAAGGGAGGGAGGGAAGGTCCCTGGTTCTTTTAATTTTCATCACATATAAACTTAATCTCGATGTTGTAGGTGGTGGATCTCGTGAGACGAGCAATTCCCATTGCTTTGATGCCCGAAGACGACCCGACAAGGAGCGAGCTAAAGCAGCTACAAGAAAAGAAGGAAGAAATTGACATATTGGCACATAAGCAGGTACGACGAATACTGTGGACTGGTCTAGGCTTAGCCATCCTGCAAGTTGGGCTGTTCTTCAGGCTGACATTCTGGGAATTTTCATGGGATGTAATGGAACCAATAGCATTTTTCACCACAACAACTGGATTAGTCATAGGCTATGCTTATTTCCTGATCACTTCAAGAGATCCCACTTACCAAGACCTGTTGAAGAGGCTTTTCCTCTCAAGGCAAAGGAAGCTGTTCAAGAAGCATTCTTTTGATGTTTGCAGATTCAAGGAGTTGCAGAAAAAATGTAAGACGCCTCTCGACGCCACCGCGAATATTAAGAACCGGGTGGGCGTAGATCTGGACCTTGAGGATTGTTTGAGTAGAGATTAACACTATTGTAACCCTTCCTTTATTAGACAGGTAAGGGGGTTAACTTGCTTTCATTCAATTTTTGAAGTTTAGAGTTATAAAGTAGTAAAGAATACTTCTTTGTTCTTGCCTTGTTCAGTCAAATATGGAATGGAATGCTCTGAAATAATCATGGCGTTTTCAAATCTTGAATGTGGCTACTCTTTAATAAT

mRNA sequence

ATGATGTGGAGGAGATGGTGTTCTAGTGCTTGTTTATTGAGTCGGAGATTGCTGGTGAATGGAGTTCGCAGGCCTTCTTCTCCGGTATCTTCTTATGGAGATTTCGGACTTGGAGGAGTTTCTTTGATTCCATCGACGGCGGTACGGTCGTCGGCCTGGTTTTGTAACGGAGTGGTTTCGTTTTCGTCATCTGTGGATTCGGAAAAATCTGCAGGAGTTGGTAGCGATGGGGGAAAACGTGCGAGCGGTGGTCCTGGGCTTGGGCTTGGGCTTGGCTCTGTGAATGAAGAATCTGGTAATGAAATTAGTGGAGAGACGATATCGTTTGCGGAAGCCAAAAGGCTGATGAGATTGGTAAATGTTGAGGCGCTGAAGATGAAATTGGGGACTGATGGGAAGGAGGCCATTGGATACTCGGAGCTTATCGAAGCTTGCAAAAGCATGGGCATCGCTAGGTCTCAGGACGAAGCTGCTGCGTTTGCTCGAGTCCTCGATGAGGCTGGTGTGGTTCTTCTGTTTCGCGATAAGGTCTATCTTCATCCTGACAAGGTGGTGGATCTCGTGAGACGAGCAATTCCCATTGCTTTGATGCCCGAAGACGACCCGACAAGGAGCGAGCTAAAGCAGCTACAAGAAAAGAAGGAAGAAATTGACATATTGGCACATAAGCAGGTACGACGAATACTGTGGACTGGTCTAGGCTTAGCCATCCTGCAAGTTGGGCTGTTCTTCAGGCTGACATTCTGGGAATTTTCATGGGATGTAATGGAACCAATAGCATTTTTCACCACAACAACTGGATTAGTCATAGGCTATGCTTATTTCCTGATCACTTCAAGAGATCCCACTTACCAAGACCTGTTGAAGAGGCTTTTCCTCTCAAGGCAAAGGAAGCTGTTCAAGAAGCATTCTTTTGATGTTTGCAGATTCAAGGAGTTGCAGAAAAAATGTAAGACGCCTCTCGACGCCACCGCGAATATTAAGAACCGGGTGGGCGTAGATCTGGACCTTGAGGATTGTTTGAGTAGAGATTAACACTATTGTAACCCTTCCTTTATTAGACAGGTAAGGGGGTTAACTTGCTTTCATTCAATTTTTGAAGTTTAGAGTTATAAAGTAGTAAAGAATACTTCTTTGTTCTTGCCTTGTTCAGTCAAATATGGAATGGAATGCTCTGAAATAATCATGGCGTTTTCAAATCTTGAATGTGGCTACTCTTTAATAAT

Coding sequence (CDS)

ATGATGTGGAGGAGATGGTGTTCTAGTGCTTGTTTATTGAGTCGGAGATTGCTGGTGAATGGAGTTCGCAGGCCTTCTTCTCCGGTATCTTCTTATGGAGATTTCGGACTTGGAGGAGTTTCTTTGATTCCATCGACGGCGGTACGGTCGTCGGCCTGGTTTTGTAACGGAGTGGTTTCGTTTTCGTCATCTGTGGATTCGGAAAAATCTGCAGGAGTTGGTAGCGATGGGGGAAAACGTGCGAGCGGTGGTCCTGGGCTTGGGCTTGGGCTTGGCTCTGTGAATGAAGAATCTGGTAATGAAATTAGTGGAGAGACGATATCGTTTGCGGAAGCCAAAAGGCTGATGAGATTGGTAAATGTTGAGGCGCTGAAGATGAAATTGGGGACTGATGGGAAGGAGGCCATTGGATACTCGGAGCTTATCGAAGCTTGCAAAAGCATGGGCATCGCTAGGTCTCAGGACGAAGCTGCTGCGTTTGCTCGAGTCCTCGATGAGGCTGGTGTGGTTCTTCTGTTTCGCGATAAGGTCTATCTTCATCCTGACAAGGTGGTGGATCTCGTGAGACGAGCAATTCCCATTGCTTTGATGCCCGAAGACGACCCGACAAGGAGCGAGCTAAAGCAGCTACAAGAAAAGAAGGAAGAAATTGACATATTGGCACATAAGCAGGTACGACGAATACTGTGGACTGGTCTAGGCTTAGCCATCCTGCAAGTTGGGCTGTTCTTCAGGCTGACATTCTGGGAATTTTCATGGGATGTAATGGAACCAATAGCATTTTTCACCACAACAACTGGATTAGTCATAGGCTATGCTTATTTCCTGATCACTTCAAGAGATCCCACTTACCAAGACCTGTTGAAGAGGCTTTTCCTCTCAAGGCAAAGGAAGCTGTTCAAGAAGCATTCTTTTGATGTTTGCAGATTCAAGGAGTTGCAGAAAAAATGTAAGACGCCTCTCGACGCCACCGCGAATATTAAGAACCGGGTGGGCGTAGATCTGGACCTTGAGGATTGTTTGAGTAGAGATTAA

Protein sequence

MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVSFSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVNVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD
Homology
BLAST of CmoCh20G003220 vs. ExPASy Swiss-Prot
Match: Q8VYR0 (Calcium uniporter protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g57610 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.2e-84
Identity = 161/259 (62.16%), Postives = 200/259 (77.22%), Query Frame = 0

Query: 90  GLGSVNEESGNEISGETISFAEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELIEACKSM 149
           G   +N ES  E   + ++  EAK+LMRLVNVE +K KL G   KE + Y+ LIEA + +
Sbjct: 29  GFRCLNVESKEEDEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIEASQGL 88

Query: 150 GIARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELK 209
           GIARS DEA AFARVLD+AGV+L+FRDKVYLHP KVVDL+R+A+P+ L P+D+  R E  
Sbjct: 89  GIARSLDEAHAFARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVPLGLNPDDELIREEFD 148

Query: 210 QLQEKKEEIDILAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGL 269
           +++  KEEID+LAH+QVR+ILW GLG +++Q+G+F RLTFWEFSWDVMEPI FFTT TG+
Sbjct: 149 KMRSMKEEIDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGI 208

Query: 270 VIGYAYFLITSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCK------TPLD 329
           ++GYAYFL+TSRDPTYQD +KRLFLSRQRKL K H FD  RFKEL+ K K      +   
Sbjct: 209 IVGYAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDAERFKELENKWKITSCSSSSCH 268

Query: 330 ATANIKNRVGVDLDLEDCL 342
           A A+I+NRVGVDLDLED L
Sbjct: 269 ANASIRNRVGVDLDLEDSL 287

BLAST of CmoCh20G003220 vs. ExPASy Swiss-Prot
Match: F4I111 (Calcium uniporter protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g09575 PE=2 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.4e-83
Identity = 156/238 (65.55%), Postives = 192/238 (80.67%), Query Frame = 0

Query: 107 ISFAEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLD 166
           I+ AEAK+LMRLVNVE +K KL G   ++ + YS L+EA + MGIARS DEA  FARVLD
Sbjct: 50  ITIAEAKKLMRLVNVEDMKKKLVGVADRDVVSYSTLLEASQGMGIARSPDEAHVFARVLD 109

Query: 167 EAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQV 226
           +AGVVL+FRDKVYLHPDKVVDL+RRA+P+   PE+D  + E  +L+  KEEID+LAH+QV
Sbjct: 110 DAGVVLIFRDKVYLHPDKVVDLIRRAMPLDQNPEEDQIKEEFNKLRIMKEEIDVLAHRQV 169

Query: 227 RRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQ 286
           R+ILW GL  +++Q+GLFFRLTFWEFSWDVMEPI FF T TG+++GYAYFL+TSRDPTYQ
Sbjct: 170 RKILWCGLATSMVQIGLFFRLTFWEFSWDVMEPITFFATATGIIVGYAYFLMTSRDPTYQ 229

Query: 287 DLLKRLFLSRQRKLFKKHSFDVCRFKELQK--KCKTPLDATANIKNRVGVDLDLEDCL 342
           D +KRLFLSRQRKL K H FD  RFKEL++  K  +   A A+I+NRVG++LDLED L
Sbjct: 230 DFMKRLFLSRQRKLLKSHKFDCERFKELERLFKMTSSCHAAASIRNRVGLELDLEDAL 287

BLAST of CmoCh20G003220 vs. ExPASy Swiss-Prot
Match: O64823 (Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g23790 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 9.1e-38
Identity = 87/225 (38.67%), Postives = 135/225 (60.00%), Query Frame = 0

Query: 106 TISFAEAKRLMRLVNVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQ-DEAAAFARVL 165
           +++ A+ K+L+R   +E +K KL   G+  + Y E +  C   G A S  D  +  A++L
Sbjct: 109 SLTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVC---GEASSDPDLGSKIAKML 168

Query: 166 DEAGVVLLFRDKVYLHPDKVVDLVRRAIPI-ALMPEDDPTRSELKQLQEKKEEIDILAHK 225
           D++  V++  D V + PD+V   +   +P+  +   +DP R E K+L+ +K  ID+ AH 
Sbjct: 169 DDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVIDVKAHT 228

Query: 226 QVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPT 285
            VR+ LW GLG  ILQ   F RLTFWE SWDVMEPI F+ T+   + GYA+FL TS++P+
Sbjct: 229 LVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRTSKEPS 288

Query: 286 YQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKK-CKTPLDATANI 328
           ++   +  F ++QRKL  ++ FD+ R+ EL+K  C  P D  + I
Sbjct: 289 FEGFYQSRFEAKQRKLMNEYEFDLERYNELKKLFCSKPSDHVSKI 330

BLAST of CmoCh20G003220 vs. ExPASy Swiss-Prot
Match: Q9FJV7 (Calcium uniporter protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g42610 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.9e-35
Identity = 85/228 (37.28%), Postives = 135/228 (59.21%), Query Frame = 0

Query: 87  LGLGLGSVNEESGNEISGETISFAEAKRLMRLVNVEALKMKLGTDGKEAIGYSELIE-AC 146
           +G+ + SV+     E     +S +E K+L+RL  +E +K +L    K ++ Y E I+  C
Sbjct: 54  VGIRIESVSPPRREETPVMGLSVSETKKLLRLYQMEKVKERLREIPKSSVSYWEFIQICC 113

Query: 147 KSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRS 206
           +S G   + ++ +  A+ LD +G V++  D V+LHP ++   +   I    +  +DP + 
Sbjct: 114 ESCG---NDEQGSQMAKSLDHSGCVVVLGDIVFLHPHQIAKSMEAMIKQTSVLPNDPRKE 173

Query: 207 ELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTT 266
           EL QL   K+ IDI A + V+  L+ GLG   +Q   F RLTFWE SWDVMEPI FF TT
Sbjct: 174 ELVQLATTKKSIDIEARRIVQAELYCGLGFLAVQTIGFMRLTFWELSWDVMEPICFFVTT 233

Query: 267 TGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKEL 314
              ++GY +FL TS +P+++ L ++ F ++Q+KL ++H FD  R+ EL
Sbjct: 234 IHFILGYIFFLRTSTEPSFEGLFRQRFKTKQKKLMERHGFDFLRYNEL 278

BLAST of CmoCh20G003220 vs. ExPASy Swiss-Prot
Match: Q1PE15 (Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g36820 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.2e-35
Identity = 81/210 (38.57%), Postives = 125/210 (59.52%), Query Frame = 0

Query: 107 ISFAEAKRLMRLVNVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDE 166
           ++  + K+L+R   +E +K KL   GK  I YS+ +  C    +  SQ   A  A++LD+
Sbjct: 112 LTVQDVKKLLRAAEIEVIKTKLMETGKIWIRYSDFLGVCSDSSLDPSQ--GALIAKMLDD 171

Query: 167 AGVVLLFRDKVYLHPDKVVDLVRRAIPIA-LMPEDDPTRSELKQLQEKKEEIDILAHKQV 226
           +G V++  + V L P ++   +   +P++ +   +DP R EL +L+  K  ID  AH  V
Sbjct: 172 SGNVIVMGNSVCLRPHQLTKSIEGLLPLSQIHNPNDPRRKELNELEAIKTVIDQKAHSLV 231

Query: 227 RRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQ 286
           RR LW GLG  I+Q   F RLTFW+ +WDVMEPI F+ ++   + GY +FL TSR+P++Q
Sbjct: 232 RRELWAGLGYLIIQTAGFMRLTFWDLTWDVMEPICFYVSSVYFMAGYTFFLKTSREPSFQ 291

Query: 287 DLLKRLFLSRQRKLFKKHSFDVCRFKELQK 316
              +  F ++QRKL +   FDV R+ EL+K
Sbjct: 292 GFYQSRFEAKQRKLMQSEDFDVGRYDELKK 319

BLAST of CmoCh20G003220 vs. ExPASy TrEMBL
Match: A0A6J1FV50 (Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111448718 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 8.5e-188
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60
           MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS
Sbjct: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60

Query: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN 120
           FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN
Sbjct: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN 120

Query: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180
           VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH
Sbjct: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180

Query: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240
           PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV
Sbjct: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240

Query: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300
           GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF
Sbjct: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300

Query: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 344

BLAST of CmoCh20G003220 vs. ExPASy TrEMBL
Match: A0A6J1JHV8 (Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111484499 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.2e-179
Identity = 333/344 (96.80%), Postives = 334/344 (97.09%), Query Frame = 0

Query: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60
           MMWRRWCSS  LLSR LLVNGVRRPSSPVSSYG FGLGGVSLI STAVRSSAWFC  VVS
Sbjct: 1   MMWRRWCSSVSLLSRSLLVNGVRRPSSPVSSYGAFGLGGVSLISSTAVRSSAWFCTRVVS 60

Query: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN 120
           FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGN ISGETISFAEAKRLMRLVN
Sbjct: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNGISGETISFAEAKRLMRLVN 120

Query: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180
           VEALKMKLGTDGKEAIGY ELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH
Sbjct: 121 VEALKMKLGTDGKEAIGYLELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180

Query: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240
           PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV
Sbjct: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240

Query: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300
            LFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF
Sbjct: 241 ALFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300

Query: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           KKHSFDVCRFKELQKKCKTPL+ATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 KKHSFDVCRFKELQKKCKTPLNATANIKNRVGVDLDLEDCLSRD 344

BLAST of CmoCh20G003220 vs. ExPASy TrEMBL
Match: A0A6J1KZX2 (Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111499768 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 4.1e-150
Identity = 286/344 (83.14%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60
           MMWRRWCS ACL SRRLL+NG     SPV S G FGLGG+SLIPST  RSSAWF   +VS
Sbjct: 1   MMWRRWCSCACLSSRRLLMNGAPGVFSPVPSNGTFGLGGISLIPSTTERSSAWFYTRMVS 60

Query: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN 120
           FSSS DSE SAGV S+ G                  +SG  I GET+SFAEAKRLMRLVN
Sbjct: 61  FSSSGDSENSAGVKSESG------------------DSG--IGGETVSFAEAKRLMRLVN 120

Query: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180
           VEALKMKLGT+GKEAIGYSEL+EACKSMG+ARS DEAAAFARVLDEAGVVLLFRDKVYLH
Sbjct: 121 VEALKMKLGTEGKEAIGYSELLEACKSMGMARSNDEAAAFARVLDEAGVVLLFRDKVYLH 180

Query: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240
           PDKVVDLVRRAIPIALMPEDDPTRSEL QLQEKKE+ID+LAHKQVRRILWTGLGLAI+QV
Sbjct: 181 PDKVVDLVRRAIPIALMPEDDPTRSELMQLQEKKEDIDVLAHKQVRRILWTGLGLAIVQV 240

Query: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300
           GLFFRLTFWEFSWDVMEPIAFFTTTTGLV+GYAYFL TSRDPTYQDLLKRLFLSRQRKLF
Sbjct: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVMGYAYFLFTSRDPTYQDLLKRLFLSRQRKLF 300

Query: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           KKHSFDVCRFKELQKKCK PLDAT NIKNRVGVDLDLEDCLSRD
Sbjct: 301 KKHSFDVCRFKELQKKCKMPLDATTNIKNRVGVDLDLEDCLSRD 324

BLAST of CmoCh20G003220 vs. ExPASy TrEMBL
Match: A0A6J1G5P5 (Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111451075 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 4.1e-150
Identity = 288/344 (83.72%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60
           MMWRRWCS ACL SRRLL+NG     SPV S G FGLGG+SLIPST VRSSAWF   VVS
Sbjct: 1   MMWRRWCSCACLSSRRLLMNGAPGVFSPVPSNGTFGLGGISLIPSTTVRSSAWFYTRVVS 60

Query: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN 120
           FSSS DSE SA     G K  SG  G+G               GET+S AEAKRLMRLVN
Sbjct: 61  FSSSGDSENSA-----GDKSESGDFGIG---------------GETVSSAEAKRLMRLVN 120

Query: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180
           VEALKMKLGT+GKEAIGYSEL+EACKSMG+ARS DEAAAFARVLDEAGVVLLFRDKVYLH
Sbjct: 121 VEALKMKLGTEGKEAIGYSELLEACKSMGMARSNDEAAAFARVLDEAGVVLLFRDKVYLH 180

Query: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240
           PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKE+ID+LAHKQVRRILWTGLGLAI+QV
Sbjct: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEDIDVLAHKQVRRILWTGLGLAIVQV 240

Query: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300
           GLFFRLTFWEFSWDVMEPIAFFTTTTGLV+GYAYFL TSRDPTYQDLLKRLFLSRQRKLF
Sbjct: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVMGYAYFLFTSRDPTYQDLLKRLFLSRQRKLF 300

Query: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           KKHSFDVCRFKELQKKCK PLDATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 KKHSFDVCRFKELQKKCKMPLDATANIKNRVGVDLDLEDCLSRD 324

BLAST of CmoCh20G003220 vs. ExPASy TrEMBL
Match: A0A0A0KD48 (Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_6G133830 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.7e-149
Identity = 289/345 (83.77%), Postives = 302/345 (87.54%), Query Frame = 0

Query: 2   MWRRWCSSA-CLLSRRLLVNGVRRPSSPVSSYGDFGL-GGVSLIPSTAVRSSAWFCNGVV 61
           MWR+ CSSA  L  RRL VNG+   SSPV  YG  GL  GVS IPS  VRS+ WFC G+ 
Sbjct: 1   MWRKCCSSATSLFIRRLFVNGLSGSSSPV-YYGTLGLSAGVSFIPSPPVRSNGWFCTGLA 60

Query: 62  SFSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLV 121
            FSSSVDS    GV  D  K  SGG   G G+GSVN ESGN I+GE ISF EAKRLMRLV
Sbjct: 61  LFSSSVDSGNFTGVRRDVDKSESGG---GPGIGSVNGESGNGITGEAISFGEAKRLMRLV 120

Query: 122 NVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYL 181
           NVEALK KLGTDGKEAIGY ELIEACKSMG+ARS+DEAAAFARVLDEAGVVLLFRDKVYL
Sbjct: 121 NVEALKTKLGTDGKEAIGYLELIEACKSMGVARSEDEAAAFARVLDEAGVVLLFRDKVYL 180

Query: 182 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQ 241
           HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEID+LAHKQVRRILWTGLGLA+LQ
Sbjct: 181 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAVLQ 240

Query: 242 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKL 301
           VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFL TSRDPTYQDLLKRLFLSRQRKL
Sbjct: 241 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKL 300

Query: 302 FKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           FKKHSFDVCRFKELQKKCK+PLDATANIKNRVG+DL+LEDCLSRD
Sbjct: 301 FKKHSFDVCRFKELQKKCKSPLDATANIKNRVGIDLELEDCLSRD 341

BLAST of CmoCh20G003220 vs. NCBI nr
Match: XP_022944197.1 (calcium uniporter protein 5, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 666.0 bits (1717), Expect = 1.8e-187
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60
           MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS
Sbjct: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60

Query: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN 120
           FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN
Sbjct: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN 120

Query: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180
           VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH
Sbjct: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180

Query: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240
           PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV
Sbjct: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240

Query: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300
           GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF
Sbjct: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300

Query: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 344

BLAST of CmoCh20G003220 vs. NCBI nr
Match: KAG6570611.1 (Calcium uniporter protein 5, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 660.2 bits (1702), Expect = 9.6e-186
Identity = 342/344 (99.42%), Postives = 342/344 (99.42%), Query Frame = 0

Query: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60
           MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS
Sbjct: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60

Query: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN 120
           FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESG  ISGETISFAEAKRLMRLVN
Sbjct: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGYGISGETISFAEAKRLMRLVN 120

Query: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180
           VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH
Sbjct: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180

Query: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240
           PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV
Sbjct: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240

Query: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300
           GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF
Sbjct: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300

Query: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 344

BLAST of CmoCh20G003220 vs. NCBI nr
Match: XP_023512709.1 (calcium uniporter protein 5, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 653.7 bits (1685), Expect = 9.0e-184
Identity = 341/346 (98.55%), Postives = 341/346 (98.55%), Query Frame = 0

Query: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60
           MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFC  VVS
Sbjct: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCTRVVS 60

Query: 61  FSSSVDSEKSAGVGSDGGKRASGGP--GLGLGLGSVNEESGNEISGETISFAEAKRLMRL 120
           FSSSVDSEKSAGVGSDGGKRASGGP  GLGLGLGSVNEESGNEISGETISFAEAKRLMRL
Sbjct: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGLGSVNEESGNEISGETISFAEAKRLMRL 120

Query: 121 VNVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVY 180
           VNVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVY
Sbjct: 121 VNVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVY 180

Query: 181 LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAIL 240
           LHPDKVVDLVRRAIPIALMPEDDPTRSELK LQEKKEEIDILAHKQVRRILWTGLGLAIL
Sbjct: 181 LHPDKVVDLVRRAIPIALMPEDDPTRSELKHLQEKKEEIDILAHKQVRRILWTGLGLAIL 240

Query: 241 QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRK 300
           QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRK
Sbjct: 241 QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRK 300

Query: 301 LFKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           LFKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 LFKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 346

BLAST of CmoCh20G003220 vs. NCBI nr
Match: XP_022986898.1 (calcium uniporter protein 5, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 637.5 bits (1643), Expect = 6.7e-179
Identity = 333/344 (96.80%), Postives = 334/344 (97.09%), Query Frame = 0

Query: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLGGVSLIPSTAVRSSAWFCNGVVS 60
           MMWRRWCSS  LLSR LLVNGVRRPSSPVSSYG FGLGGVSLI STAVRSSAWFC  VVS
Sbjct: 1   MMWRRWCSSVSLLSRSLLVNGVRRPSSPVSSYGAFGLGGVSLISSTAVRSSAWFCTRVVS 60

Query: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLVN 120
           FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGN ISGETISFAEAKRLMRLVN
Sbjct: 61  FSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNGISGETISFAEAKRLMRLVN 120

Query: 121 VEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180
           VEALKMKLGTDGKEAIGY ELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH
Sbjct: 121 VEALKMKLGTDGKEAIGYLELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYLH 180

Query: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240
           PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV
Sbjct: 181 PDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQV 240

Query: 241 GLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300
            LFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF
Sbjct: 241 ALFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKLF 300

Query: 301 KKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           KKHSFDVCRFKELQKKCKTPL+ATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 KKHSFDVCRFKELQKKCKTPLNATANIKNRVGVDLDLEDCLSRD 344

BLAST of CmoCh20G003220 vs. NCBI nr
Match: XP_038902671.1 (calcium uniporter protein 5, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 566.6 bits (1459), Expect = 1.4e-157
Identity = 301/345 (87.25%), Postives = 310/345 (89.86%), Query Frame = 0

Query: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPVSSYGDFGLG-GVSLIPSTAVRSSAWFCNGVV 60
           MMWR+WCSS  LLSRRLLVNGV   S   S YG  GLG GVSL PS AVRSS WFC  V 
Sbjct: 30  MMWRKWCSSVSLLSRRLLVNGVPGNS---SYYGTLGLGRGVSLNPSPAVRSSTWFCTRVA 89

Query: 61  SFSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLV 120
           SFSSSVDS  S G+  D  K  SGGP    GLGSVNEESGN I GETISF EAKRLMRLV
Sbjct: 90  SFSSSVDSGNSTGIRRDAEKSESGGP----GLGSVNEESGNGIGGETISFGEAKRLMRLV 149

Query: 121 NVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYL 180
           NVEALKMKLGTDGKEAIGY+EL+EACKSMG+ARSQDEAAAFARVLDEAGV+LLFRDKVYL
Sbjct: 150 NVEALKMKLGTDGKEAIGYTELLEACKSMGVARSQDEAAAFARVLDEAGVILLFRDKVYL 209

Query: 181 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQ 240
           HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEID+LAHKQVRRILWTGLGLAILQ
Sbjct: 210 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQ 269

Query: 241 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKL 300
           VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFL TSRDPTYQDLLKRLFLSRQRKL
Sbjct: 270 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKL 329

Query: 301 FKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 345
           FKKHSFDVCRFKELQKKCK+PLDATANIKNRVGVDL+LEDCLSRD
Sbjct: 330 FKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLELEDCLSRD 367

BLAST of CmoCh20G003220 vs. TAIR 10
Match: AT1G57610.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 313.9 bits (803), Expect = 1.6e-85
Identity = 161/259 (62.16%), Postives = 200/259 (77.22%), Query Frame = 0

Query: 90  GLGSVNEESGNEISGETISFAEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELIEACKSM 149
           G   +N ES  E   + ++  EAK+LMRLVNVE +K KL G   KE + Y+ LIEA + +
Sbjct: 29  GFRCLNVESKEEDEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIEASQGL 88

Query: 150 GIARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELK 209
           GIARS DEA AFARVLD+AGV+L+FRDKVYLHP KVVDL+R+A+P+ L P+D+  R E  
Sbjct: 89  GIARSLDEAHAFARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVPLGLNPDDELIREEFD 148

Query: 210 QLQEKKEEIDILAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGL 269
           +++  KEEID+LAH+QVR+ILW GLG +++Q+G+F RLTFWEFSWDVMEPI FFTT TG+
Sbjct: 149 KMRSMKEEIDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGI 208

Query: 270 VIGYAYFLITSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCK------TPLD 329
           ++GYAYFL+TSRDPTYQD +KRLFLSRQRKL K H FD  RFKEL+ K K      +   
Sbjct: 209 IVGYAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDAERFKELENKWKITSCSSSSCH 268

Query: 330 ATANIKNRVGVDLDLEDCL 342
           A A+I+NRVGVDLDLED L
Sbjct: 269 ANASIRNRVGVDLDLEDSL 287

BLAST of CmoCh20G003220 vs. TAIR 10
Match: AT1G57610.2 (Protein of unknown function (DUF607) )

HSP 1 Score: 313.9 bits (803), Expect = 1.6e-85
Identity = 161/259 (62.16%), Postives = 200/259 (77.22%), Query Frame = 0

Query: 90  GLGSVNEESGNEISGETISFAEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELIEACKSM 149
           G   +N ES  E   + ++  EAK+LMRLVNVE +K KL G   KE + Y+ LIEA + +
Sbjct: 29  GFRCLNVESKEEDEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIEASQGL 88

Query: 150 GIARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELK 209
           GIARS DEA AFARVLD+AGV+L+FRDKVYLHP KVVDL+R+A+P+ L P+D+  R E  
Sbjct: 89  GIARSLDEAHAFARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVPLGLNPDDELIREEFD 148

Query: 210 QLQEKKEEIDILAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGL 269
           +++  KEEID+LAH+QVR+ILW GLG +++Q+G+F RLTFWEFSWDVMEPI FFTT TG+
Sbjct: 149 KMRSMKEEIDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGI 208

Query: 270 VIGYAYFLITSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCK------TPLD 329
           ++GYAYFL+TSRDPTYQD +KRLFLSRQRKL K H FD  RFKEL+ K K      +   
Sbjct: 209 IVGYAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDAERFKELENKWKITSCSSSSCH 268

Query: 330 ATANIKNRVGVDLDLEDCL 342
           A A+I+NRVGVDLDLED L
Sbjct: 269 ANASIRNRVGVDLDLEDSL 287

BLAST of CmoCh20G003220 vs. TAIR 10
Match: AT1G09575.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 310.5 bits (794), Expect = 1.7e-84
Identity = 156/238 (65.55%), Postives = 192/238 (80.67%), Query Frame = 0

Query: 107 ISFAEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLD 166
           I+ AEAK+LMRLVNVE +K KL G   ++ + YS L+EA + MGIARS DEA  FARVLD
Sbjct: 50  ITIAEAKKLMRLVNVEDMKKKLVGVADRDVVSYSTLLEASQGMGIARSPDEAHVFARVLD 109

Query: 167 EAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQV 226
           +AGVVL+FRDKVYLHPDKVVDL+RRA+P+   PE+D  + E  +L+  KEEID+LAH+QV
Sbjct: 110 DAGVVLIFRDKVYLHPDKVVDLIRRAMPLDQNPEEDQIKEEFNKLRIMKEEIDVLAHRQV 169

Query: 227 RRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQ 286
           R+ILW GL  +++Q+GLFFRLTFWEFSWDVMEPI FF T TG+++GYAYFL+TSRDPTYQ
Sbjct: 170 RKILWCGLATSMVQIGLFFRLTFWEFSWDVMEPITFFATATGIIVGYAYFLMTSRDPTYQ 229

Query: 287 DLLKRLFLSRQRKLFKKHSFDVCRFKELQK--KCKTPLDATANIKNRVGVDLDLEDCL 342
           D +KRLFLSRQRKL K H FD  RFKEL++  K  +   A A+I+NRVG++LDLED L
Sbjct: 230 DFMKRLFLSRQRKLLKSHKFDCERFKELERLFKMTSSCHAAASIRNRVGLELDLEDAL 287

BLAST of CmoCh20G003220 vs. TAIR 10
Match: AT1G57610.3 (Protein of unknown function (DUF607) )

HSP 1 Score: 218.4 bits (555), Expect = 9.0e-57
Identity = 109/181 (60.22%), Postives = 141/181 (77.90%), Query Frame = 0

Query: 90  GLGSVNEESGNEISGETISFAEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELIEACKSM 149
           G   +N ES  E   + ++  EAK+LMRLVNVE +K KL G   KE + Y+ LIEA + +
Sbjct: 29  GFRCLNVESKEEDEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIEASQGL 88

Query: 150 GIARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELK 209
           GIARS DEA AFARVLD+AGV+L+FRDKVYLHP KVVDL+R+A+P+ L P+D+  R E  
Sbjct: 89  GIARSLDEAHAFARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVPLGLNPDDELIREEFD 148

Query: 210 QLQEKKEEIDILAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGL 269
           +++  KEEID+LAH+QVR+ILW GLG +++Q+G+F RLTFWEFSWDVMEPI FFTT TG+
Sbjct: 149 KMRSMKEEIDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGI 208

BLAST of CmoCh20G003220 vs. TAIR 10
Match: AT2G23790.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 159.1 bits (401), Expect = 6.5e-39
Identity = 87/225 (38.67%), Postives = 135/225 (60.00%), Query Frame = 0

Query: 106 TISFAEAKRLMRLVNVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQ-DEAAAFARVL 165
           +++ A+ K+L+R   +E +K KL   G+  + Y E +  C   G A S  D  +  A++L
Sbjct: 109 SLTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVC---GEASSDPDLGSKIAKML 168

Query: 166 DEAGVVLLFRDKVYLHPDKVVDLVRRAIPI-ALMPEDDPTRSELKQLQEKKEEIDILAHK 225
           D++  V++  D V + PD+V   +   +P+  +   +DP R E K+L+ +K  ID+ AH 
Sbjct: 169 DDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVIDVKAHT 228

Query: 226 QVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPT 285
            VR+ LW GLG  ILQ   F RLTFWE SWDVMEPI F+ T+   + GYA+FL TS++P+
Sbjct: 229 LVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRTSKEPS 288

Query: 286 YQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKK-CKTPLDATANI 328
           ++   +  F ++QRKL  ++ FD+ R+ EL+K  C  P D  + I
Sbjct: 289 FEGFYQSRFEAKQRKLMNEYEFDLERYNELKKLFCSKPSDHVSKI 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYR02.2e-8462.16Calcium uniporter protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
F4I1112.4e-8365.55Calcium uniporter protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
O648239.1e-3838.67Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9FJV71.9e-3537.28Calcium uniporter protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q1PE154.2e-3538.57Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Match NameE-valueIdentityDescription
A0A6J1FV508.5e-188100.00Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111448718 PE=3 SV=... [more]
A0A6J1JHV83.2e-17996.80Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111484499 PE=3 SV=1[more]
A0A6J1KZX24.1e-15083.14Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111499768 PE=3 SV=1[more]
A0A6J1G5P54.1e-15083.72Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111451075 PE=3 SV=... [more]
A0A0A0KD482.7e-14983.77Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_6G133830 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022944197.11.8e-187100.00calcium uniporter protein 5, mitochondrial-like [Cucurbita moschata][more]
KAG6570611.19.6e-18699.42Calcium uniporter protein 5, mitochondrial, partial [Cucurbita argyrosperma subs... [more]
XP_023512709.19.0e-18498.55calcium uniporter protein 5, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_022986898.16.7e-17996.80calcium uniporter protein 5, mitochondrial-like [Cucurbita maxima][more]
XP_038902671.11.4e-15787.25calcium uniporter protein 5, mitochondrial-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G57610.11.6e-8562.16Protein of unknown function (DUF607) [more]
AT1G57610.21.6e-8562.16Protein of unknown function (DUF607) [more]
AT1G09575.11.7e-8465.55Protein of unknown function (DUF607) [more]
AT1G57610.39.0e-5760.22Protein of unknown function (DUF607) [more]
AT2G23790.16.5e-3938.67Protein of unknown function (DUF607) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006769Calcium uniporter protein, C-terminalPFAMPF04678MCUcoord: 154..313
e-value: 2.5E-50
score: 171.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 66..86
NoneNo IPR availablePANTHERPTHR13462:SF41CALCIUM UNIPORTER PROTEIN 6, MITOCHONDRIALcoord: 101..327
IPR039055MCU familyPANTHERPTHR13462CALCIUM UNIPORTER PROTEIN, MITOCHONDRIALcoord: 101..327

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G003220.1CmoCh20G003220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036444 calcium import into the mitochondrion
biological_process GO:0051560 mitochondrial calcium ion homeostasis
cellular_component GO:0031305 integral component of mitochondrial inner membrane
cellular_component GO:1990246 uniplex complex
molecular_function GO:0005262 calcium channel activity
molecular_function GO:0015292 uniporter activity