CmoCh20G003140 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh20G003140
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Ferrochelatase
Location	Cmo_Chr20: 1542031 .. 1543597 (-)
RNA-Seq Expression	CmoCh20G003140
Synteny	CmoCh20G003140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCATTTTAAGAGACTCGTAATATGGTTGAGTGGGAGTTCATATGATATAATACGGTTGCCACGATTGTTCCGATTCCTCCAAAGACCATTGGCACACTTTATTTCTGTTCTAAGATCGCCCAAGAGTAGAGAAGGCTATGCCTCAATGGGGCGGTGGTTCTCCTCTGCCAAAGATAACTGACGCACAGGCAAGAAATTCTTCTTCTTCTTCTTGCCATTTATTTTAATGCACACCATTATTGCTGTTTCACAATCGAGATCACAAAAAAAGATTAGAGGAATACTGAGAATAATGTCTGCATTTTCACTCTCTTGTTCAATGCATGGTTTGTGAGTGCAATGGGGGATGCATGATTGCTTGAAGATATGTTGCCTTCTGTTTATGCGATTTTTTTTTTTTAGGAAATAGGAACTACTTCATTCATTCAATCAATCAAAAGAAATCATATTGGCCTGATGAAGAAAGGGACAAGGTGTTCCATTCCCAAAAATTAGTAAGACTACATAATAGCCCTGCAATCTAAGTTATGATGATTGCTCCTATTGGAATCTATGACTTGTACAAGAATTCTAGTTGACAACAGTAATCTGAACATGAGCGTAGCTTAACCGACAAAGTATCTACCTTCAACTAAGAGGTTAAATATTCGAATCTCTCCCCCTGCATAGTAGTCTAAAGAAGTGTACTTATCTAAAAACACTTCCGCATTTCATTAAGGGGTTCGAGGGCTCCGTAGACCTCAAGGTAGGCTCTATAGTGTACTTTGTTCGAGGAAGGATTGTTGGGAGAGGGGTCCCACATCGGCTAATAGTGTACATGTTGTTGGTACGAGGCCTTATGGGAGTACCAAAAGCAAAACCATACTACTGTGGAGATTCATGGTTCCTAACGGGAACAACAATGTCTTATGGTAAGCTTACAAACTCTAAATTTTGGTACACGAACAAAATCATTGTGTATGTTTATTGATGCATCTATCAAATCTTTTCTTTTTCCTCGTAGATAAAAAAAGACAGGATATCAAAGCTTGTTGTTATTCCTCTTTATCCCCAATTTTCAATATGCACAAGTGGTTCGAACCTTGGGCTGTTGGAGGGTATCTTCCGGTAAAGGCTAGATCCTTTATGTTCATACAATATTCTGACATCCTGGATTGGTTTAAATGACAATAGAACTTTCTTTTTGGTTCATAAGTGTTACTATTCATGCCTTGGCATCAAAATGTAACTTGAACAAATACATAACCATCACTTATGAGTTGATGATACTATTTGCATATTGACTGCGTGCAGATTACCTAGTTTAGAAATCAAAATCATATTCAGTCAATTAAATTTTTATGCTATACTTCATTTGTAGCTTTGTAAGTGAGAACATAGAAACTTTGGAAGAAATAGACGTGGAATATAAAGAACTGGCTTTAAAATCTAGGATAAGAAAATGGGGACGTGTTCCAGCGTTAGGATGTGAACCTACTTTCATAACAGATTTGGCTGATGCTGTAATTGAGAGTCTTCCATATGTCAGAGCAATGGCTGTCTCAAATCTTGAAGCTCGCCAG

mRNA sequence

ATGGGCCATTTTAAGAGACTCGTAATATGGTTGAGTGGGAGTTCATATGATATAATACGGTTGCCACGATTGTTCCGATTCCTCCAAAGACCATTGGCACACTTTATTTCTGTTCTAAGATCGCCCAAGAGTAGAGAAGGCTATGCCTCAATGGGGCGGAACTACTTCATTCATTCAATCAATCAAAAGAAATCATATTGGCCTGATGAAGAAAGGGACAAGGTGTTCCATTCCCAAAAATTAATAAAAAAAGACAGGATATCAAAGCTTGTTGTTATTCCTCTTTATCCCCAATTTTCAATATGCACAAGTGGTTCGAACCTTGGGCTGTTGGAGGGTATCTTCCGCTTTGTAAGTGAGAACATAGAAACTTTGGAAGAAATAGACGTGGAATATAAAGAACTGGCTTTAAAATCTAGGATAAGAAAATGGGGACGTGTTCCAGCGTTAGGATGTGAACCTACTTTCATAACAGATTTGGCTGATGCTGTAATTGAGAGTCTTCCATATGTCAGAGCAATGGCTGTCTCAAATCTTGAAGCTCGCCAG

Coding sequence (CDS)

ATGGGCCATTTTAAGAGACTCGTAATATGGTTGAGTGGGAGTTCATATGATATAATACGGTTGCCACGATTGTTCCGATTCCTCCAAAGACCATTGGCACACTTTATTTCTGTTCTAAGATCGCCCAAGAGTAGAGAAGGCTATGCCTCAATGGGGCGGAACTACTTCATTCATTCAATCAATCAAAAGAAATCATATTGGCCTGATGAAGAAAGGGACAAGGTGTTCCATTCCCAAAAATTAATAAAAAAAGACAGGATATCAAAGCTTGTTGTTATTCCTCTTTATCCCCAATTTTCAATATGCACAAGTGGTTCGAACCTTGGGCTGTTGGAGGGTATCTTCCGCTTTGTAAGTGAGAACATAGAAACTTTGGAAGAAATAGACGTGGAATATAAAGAACTGGCTTTAAAATCTAGGATAAGAAAATGGGGACGTGTTCCAGCGTTAGGATGTGAACCTACTTTCATAACAGATTTGGCTGATGCTGTAATTGAGAGTCTTCCATATGTCAGAGCAATGGCTGTCTCAAATCTTGAAGCTCGCCAG

Protein sequence

MGHFKRLVIWLSGSSYDIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQKKSYWPDEERDKVFHSQKLIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFRFVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVRAMAVSNLEARQ

Homology

[Close]

BLAST of CmoCh20G003140 vs. ExPASy Swiss-Prot
Match: Q0DIV0 (Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0361200 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 3.6e-41
Identity = 123/311 (39.55%), Postives = 141/311 (45.34%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQ+PLA FISV+R+PKS+EGYAS+G    +  I        +K+    +
Sbjct: 142 DIIRLPRLFRFLQKPLAQFISVVRAPKSKEGYASIGGGSPLRQITDAQAEALRKALCDKD 201

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              KV+   +            IK+D I+KLVV+PLYPQFSI TSGS+L LLEGIFR   
Sbjct: 202 IPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLEGIFREDE 261

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 262 YLVNMQHTVIPSWYQREGYIKAMATLIEKELRTFSEPQKVMIFFSAHGVPLAYVEEAGDP 321

[Close]

BLAST of CmoCh20G003140 vs. ExPASy Swiss-Prot
Match: A2Y3Q5 (Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=HEMH PE=2 SV=2)

HSP 1 Score: 169.5 bits (428), Expect = 3.6e-41
Identity = 123/311 (39.55%), Postives = 141/311 (45.34%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQ+PLA FISV+R+PKS+EGYAS+G    +  I        +K+    +
Sbjct: 142 DIIRLPRLFRFLQKPLAQFISVVRAPKSKEGYASIGGGSPLRQITDAQAEALRKALCDKD 201

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              KV+   +            IK+D I+KLVV+PLYPQFSI TSGS+L LLEGIFR   
Sbjct: 202 IPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLEGIFREDE 261

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 262 YLVNMQHTVIPSWYQREGYIKAMATLIEKELRTFSEPQKVMIFFSAHGVPLAYVEEAGDP 321

[Close]

BLAST of CmoCh20G003140 vs. ExPASy Swiss-Prot
Match: O04921 (Ferrochelatase-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FC2 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.4e-37
Identity = 117/311 (37.62%), Postives = 135/311 (43.41%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLP +F+FLQ+PLA FISV R+PKS+EGYAS+G    +  I        +K  W   
Sbjct: 129 DIIRLPPVFQFLQKPLAQFISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKN 188

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              KV+   +            IK+D I+KLVV+PLYPQFSI TSGS+L LLE IFR   
Sbjct: 189 VPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDE 248

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 249 YLVNMQHTVIPSWYQREGYIKAMANLIQSELGKFGSPNQVVIFFSAHGVPLAYVEEAGDP 308

[Close]

BLAST of CmoCh20G003140 vs. ExPASy Swiss-Prot
Match: Q69TB1 (Ferrochelatase-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0297000 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 4.6e-28
Identity = 96/312 (30.77%), Postives = 124/312 (39.74%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQKKS----------- 76
           DIIRLPRLFRFLQRPLA  IS  R+PKS+EGYAS+G    +  I  +++           
Sbjct: 135 DIIRLPRLFRFLQRPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQANALKVALKKKN 194

Query: 77  ----------YWPDEERDKVFHSQKLIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIF 136
                     YW     + +      IKKD+I+KLVV+PLYPQ+SI TSGS++ +L+ I 
Sbjct: 195 LNANIYVGMRYWYPFTEEAI----DQIKKDKITKLVVLPLYPQYSISTSGSSIRVLQNIV 254

Query: 137 R----------------------------------------------------------- 181
           +                                                           
Sbjct: 255 KEDSYFAGLPISIIESWYQRDGYVKSMADLIEKELSIFSNPEEVMIFFSAHGVPLTYVTD 314

[Close]

BLAST of CmoCh20G003140 vs. ExPASy Swiss-Prot
Match: P42045 (Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 4.6e-28
Identity = 97/312 (31.09%), Postives = 124/312 (39.74%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQKKS----------- 76
           DIIRLPRLFRFLQRPLA  IS  R+PKS EGYAS+G    +  I  +++           
Sbjct: 137 DIIRLPRLFRFLQRPLAKLISTFRAPKSNEGYASIGGGSPLRKITDEQANALKVALKSKN 196

Query: 77  ----------YWPDEERDKVFHSQKLIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIF 136
                     YW     + +      IKKD+I+KLVV+PLYPQ+SI TSGS++ +L+ I 
Sbjct: 197 LEADIYVGMRYWYPFTEEAI----DQIKKDKITKLVVLPLYPQYSISTSGSSIRVLQNIV 256

Query: 137 R----------------------------------------------------------- 181
           +                                                           
Sbjct: 257 KEDPYFAGLPISIIESWYQREGYVKSMADLIEKELSVFSNPEEVMIFFSAHGVPLTYVKD 316

[Close]

BLAST of CmoCh20G003140 vs. ExPASy TrEMBL
Match: A0A061DNX9 (Ferrochelatase 2 isoform 3 OS=Theobroma cacao OX=3641 GN=TCM_003334 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 5.4e-48
Identity = 123/228 (53.95%), Postives = 137/228 (60.09%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQ+PLA FISVLR+PKS+EGYAS+G    +  I        +KS W   
Sbjct: 133 DIIRLPRLFRFLQKPLAQFISVLRAPKSKEGYASIGGGSPLRRITDAQAEELRKSLWVKN 192

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              KV+   +            IKKD I+KLVV+PLYPQFSI TSGS+L LLE IFR   
Sbjct: 193 VPAKVYVGMRYWHPFTEEAIEQIKKDGITKLVVLPLYPQFSISTSGSSLRLLESIFRDDE 252

Query: 137 -----------------------------------------FVSENIETLEEIDVEYKEL 184
                                                    FVSE+IETLEEIDVEYKEL
Sbjct: 253 YLVNMRHTVIPSWYQREGYIKSMANLIEKELQKFDLPEKVSFVSEHIETLEEIDVEYKEL 312

[Close]

BLAST of CmoCh20G003140 vs. ExPASy TrEMBL
Match: A0A6J1L275 (Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111499733 PE=3 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 2.1e-44
Identity = 132/311 (42.44%), Postives = 144/311 (46.30%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYAS+G    +  I        KK+ W  +
Sbjct: 143 DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASIGGGSPLRKITDAQAEELKKALWKKD 202

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              +V+   +            IKKDRI+KLVV+PLYPQFSI TSGS+L LLEGIFR   
Sbjct: 203 VPAEVYVGMRYWHPFTEEAIEQIKKDRITKLVVLPLYPQFSISTSGSSLRLLEGIFREDE 262

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 263 YLVNMQHTVIPSWYQREGYIKAMADLIEKELKSFDFPEQVMVFFSAHGVPLAYVEEAGDP 322

[Close]

BLAST of CmoCh20G003140 vs. ExPASy TrEMBL
Match: A0A6J1G6H7 (Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111451226 PE=3 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 2.1e-44
Identity = 132/311 (42.44%), Postives = 144/311 (46.30%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYAS+G    +  I        KK+ W  +
Sbjct: 143 DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASIGGGSPLRKITDAQAEELKKALWQKD 202

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              +V+   +            IKKDRI+KLVV+PLYPQFSI TSGS+L LLEGIFR   
Sbjct: 203 VPAEVYVGMRYWHPFTEEAIEQIKKDRITKLVVLPLYPQFSISTSGSSLRLLEGIFREDE 262

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 263 YLVNMQHTVIPSWYQREGYIKAMADLIEKELKSFDFPEQVMVFFSAHGVPLAYVEEAGDP 322

[Close]

BLAST of CmoCh20G003140 vs. ExPASy TrEMBL
Match: A0A061DPW8 (Ferrochelatase 2 isoform 2 OS=Theobroma cacao OX=3641 GN=TCM_003334 PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.8e-44
Identity = 123/260 (47.31%), Postives = 137/260 (52.69%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQ+PLA FISVLR+PKS+EGYAS+G    +  I        +KS W   
Sbjct: 133 DIIRLPRLFRFLQKPLAQFISVLRAPKSKEGYASIGGGSPLRRITDAQAEELRKSLWVKN 192

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              KV+   +            IKKD I+KLVV+PLYPQFSI TSGS+L LLE IFR   
Sbjct: 193 VPAKVYVGMRYWHPFTEEAIEQIKKDGITKLVVLPLYPQFSISTSGSSLRLLESIFRDDE 252

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 253 YLVNMRHTVIPSWYQREGYIKSMANLIEKELQKFDLPEKSRVGPVEWLKPYTDETIVELG 312

[Close]

BLAST of CmoCh20G003140 vs. ExPASy TrEMBL
Match: A0A1S4E153 (Ferrochelatase OS=Cucumis melo OX=3656 GN=LOC103496275 PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 8.0e-44
Identity = 132/311 (42.44%), Postives = 143/311 (45.98%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQRPLA FISVLRSPKSREGYAS+G    +  I        KK+ W  +
Sbjct: 138 DIIRLPRLFRFLQRPLARFISVLRSPKSREGYASIGGGSPLRKITDAQAEELKKALWQKD 197

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              +V+   +            IKKDRISKLVV+PLYPQFSI TSGS+L LLEGIFR   
Sbjct: 198 VPAEVYVGMRYWHPFTEEAIEQIKKDRISKLVVLPLYPQFSISTSGSSLRLLEGIFREDE 257

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 258 YLVNMQHTVIPSWYQREGYIKAMADLIEKELRTFDFPEQVMVFFSAHGVPLAYVEEAGDP 317

[Close]

BLAST of CmoCh20G003140 vs. NCBI nr
Match: KAG7010455.1 (Ferrochelatase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 243.4 bits (620), Expect = 1.5e-60
Identity = 124/131 (94.66%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 53  RNYFIHSINQKKSYWPDEERDKVFHSQKLIKKDRISKLVVIPLYPQFSICTSGSNLGLLE 112
           RNYFIHSINQKKSYWPDEERDK       IKKDRISKLVVIPLYPQFSICTSGSNLGLLE
Sbjct: 14  RNYFIHSINQKKSYWPDEERDK-------IKKDRISKLVVIPLYPQFSICTSGSNLGLLE 73

Query: 113 GIFRFVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVR 172
           GIFRFVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVR
Sbjct: 74  GIFRFVSENIETLEEIDVEYKELALKSRIRKWGRVPALGCEPTFITDLADAVIESLPYVR 133

Query: 173 AMAVSNLEARQ 184
           AMAVSNLEARQ
Sbjct: 134 AMAVSNLEARQ 137

[Close]

BLAST of CmoCh20G003140 vs. NCBI nr
Match: EOX94122.1 (Ferrochelatase 2 isoform 3 [Theobroma cacao])

HSP 1 Score: 200.7 bits (509), Expect = 1.1e-47
Identity = 123/228 (53.95%), Postives = 137/228 (60.09%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQ+PLA FISVLR+PKS+EGYAS+G    +  I        +KS W   
Sbjct: 133 DIIRLPRLFRFLQKPLAQFISVLRAPKSKEGYASIGGGSPLRRITDAQAEELRKSLWVKN 192

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              KV+   +            IKKD I+KLVV+PLYPQFSI TSGS+L LLE IFR   
Sbjct: 193 VPAKVYVGMRYWHPFTEEAIEQIKKDGITKLVVLPLYPQFSISTSGSSLRLLESIFRDDE 252

Query: 137 -----------------------------------------FVSENIETLEEIDVEYKEL 184
                                                    FVSE+IETLEEIDVEYKEL
Sbjct: 253 YLVNMRHTVIPSWYQREGYIKSMANLIEKELQKFDLPEKVSFVSEHIETLEEIDVEYKEL 312

[Close]

BLAST of CmoCh20G003140 vs. NCBI nr
Match: XP_011011650.1 (PREDICTED: ferrochelatase-2, chloroplastic-like isoform X5 [Populus euphratica])

HSP 1 Score: 193.7 bits (491), Expect = 1.4e-45
Identity = 124/260 (47.69%), Postives = 141/260 (54.23%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQ+PLA FISV R+PKS+EGYAS+G    +  I        +KS W  +
Sbjct: 136 DIIRLPRLFRFLQKPLAQFISVTRAPKSKEGYASIGGGSPLRHITDAQAEELRKSLWEKQ 195

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              +V+   +            IK+DRI+KLVV+PLYPQFSI TSGS+L LLE IFR   
Sbjct: 196 VPARVYVGMRYWHPFTEEAIQQIKRDRITKLVVLPLYPQFSISTSGSSLRLLERIFREDE 255

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 256 YLVSMQHTVIPSWYQREGYIKALANLIGKELETFDHPEQSRVGPVEWLKPYTDETIIELG 315

[Close]

BLAST of CmoCh20G003140 vs. NCBI nr
Match: XP_022947333.1 (ferrochelatase-2, chloroplastic [Cucurbita moschata] >XP_022947334.1 ferrochelatase-2, chloroplastic [Cucurbita moschata] >XP_022947335.1 ferrochelatase-2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 188.7 bits (478), Expect = 4.4e-44
Identity = 132/311 (42.44%), Postives = 144/311 (46.30%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYAS+G    +  I        KK+ W  +
Sbjct: 143 DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASIGGGSPLRKITDAQAEELKKALWQKD 202

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              +V+   +            IKKDRI+KLVV+PLYPQFSI TSGS+L LLEGIFR   
Sbjct: 203 VPAEVYVGMRYWHPFTEEAIEQIKKDRITKLVVLPLYPQFSISTSGSSLRLLEGIFREDE 262

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 263 YLVNMQHTVIPSWYQREGYIKAMADLIEKELKSFDFPEQVMVFFSAHGVPLAYVEEAGDP 322

[Close]

BLAST of CmoCh20G003140 vs. NCBI nr
Match: XP_023007154.1 (ferrochelatase-2, chloroplastic [Cucurbita maxima] >XP_023007155.1 ferrochelatase-2, chloroplastic [Cucurbita maxima] >XP_023007156.1 ferrochelatase-2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 188.7 bits (478), Expect = 4.4e-44
Identity = 132/311 (42.44%), Postives = 144/311 (46.30%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYAS+G    +  I        KK+ W  +
Sbjct: 143 DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASIGGGSPLRKITDAQAEELKKALWKKD 202

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              +V+   +            IKKDRI+KLVV+PLYPQFSI TSGS+L LLEGIFR   
Sbjct: 203 VPAEVYVGMRYWHPFTEEAIEQIKKDRITKLVVLPLYPQFSISTSGSSLRLLEGIFREDE 262

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 263 YLVNMQHTVIPSWYQREGYIKAMADLIEKELKSFDFPEQVMVFFSAHGVPLAYVEEAGDP 322

[Close]

BLAST of CmoCh20G003140 vs. TAIR 10
Match: AT2G30390.1 (ferrochelatase 2 )

HSP 1 Score: 157.5 bits (397), Expect = 1.0e-38
Identity = 117/311 (37.62%), Postives = 135/311 (43.41%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLP +F+FLQ+PLA FISV R+PKS+EGYAS+G    +  I        +K  W   
Sbjct: 129 DIIRLPPVFQFLQKPLAQFISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKN 188

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              KV+   +            IK+D I+KLVV+PLYPQFSI TSGS+L LLE IFR   
Sbjct: 189 VPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDE 248

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 249 YLVNMQHTVIPSWYQREGYIKAMANLIQSELGKFGSPNQVVIFFSAHGVPLAYVEEAGDP 308

[Close]

BLAST of CmoCh20G003140 vs. TAIR 10
Match: AT2G30390.2 (ferrochelatase 2 )

HSP 1 Score: 153.7 bits (387), Expect = 1.4e-37
Identity = 117/321 (36.45%), Postives = 135/321 (42.06%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQ------KKSYWPDE 76
           DIIRLP +F+FLQ+PLA FISV R+PKS+EGYAS+G    +  I        +K  W   
Sbjct: 129 DIIRLPPVFQFLQKPLAQFISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKN 188

Query: 77  ERDKVFHSQKL-----------IKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIFR--- 136
              KV+   +            IK+D I+KLVV+PLYPQFSI TSGS+L LLE IFR   
Sbjct: 189 VPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDE 248

Query: 137 ------------------------------------------------------------ 184
                                                                       
Sbjct: 249 YLVNMQHTVIPSWYQREGYIKAMANLIQSELGKFGSPNQTCFKPKYMLQVVIFFSAHGVP 308

[Close]

BLAST of CmoCh20G003140 vs. TAIR 10
Match: AT5G26030.1 (ferrochelatase 1 )

HSP 1 Score: 122.1 bits (305), Expect = 4.7e-28
Identity = 95/310 (30.65%), Postives = 126/310 (40.65%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQKKS----------- 76
           DIIRLPR F+FLQ  +A FISV+R+PKS+EGYA++G    +  I  +++           
Sbjct: 118 DIIRLPRPFQFLQGTIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKN 177

Query: 77  ----------YWPDEERDKVFHSQKLIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIF 136
                     YW     + V    + IKKD+I++LVV+PLYPQ+SI T+GS++ +L+ +F
Sbjct: 178 IAANVYVGMRYWYPFTEEAV----QQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLF 237

Query: 137 R----------------------------------------------------------- 179
           R                                                           
Sbjct: 238 RKDPYLAGVPVAIIKSWYQRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVEN 297

[Close]

BLAST of CmoCh20G003140 vs. TAIR 10
Match: AT5G26030.2 (ferrochelatase 1 )

HSP 1 Score: 122.1 bits (305), Expect = 4.7e-28
Identity = 95/310 (30.65%), Postives = 126/310 (40.65%), Query Frame = 0


Query: 17  DIIRLPRLFRFLQRPLAHFISVLRSPKSREGYASMGRNYFIHSINQKKS----------- 76
           DIIRLPR F+FLQ  +A FISV+R+PKS+EGYA++G    +  I  +++           
Sbjct: 118 DIIRLPRPFQFLQGTIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKN 177

Query: 77  ----------YWPDEERDKVFHSQKLIKKDRISKLVVIPLYPQFSICTSGSNLGLLEGIF 136
                     YW     + V    + IKKD+I++LVV+PLYPQ+SI T+GS++ +L+ +F
Sbjct: 178 IAANVYVGMRYWYPFTEEAV----QQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLF 237

Query: 137 R----------------------------------------------------------- 179
           R                                                           
Sbjct: 238 RKDPYLAGVPVAIIKSWYQRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVEN 297

The following BLAST results are available for this feature:

BLAST of CmoCh20G003140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0DIV0	3.6e-41	39.55	Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05...	[more]
A2Y3Q5	3.6e-41	39.55	Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=HEMH P...	[more]
O04921	1.4e-37	37.62	Ferrochelatase-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FC2 PE=1 SV=1	[more]
Q69TB1	4.6e-28	30.77	Ferrochelatase-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os09...	[more]
P42045	4.6e-28	31.09	Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1	[more]

BLAST of CmoCh20G003140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A061DNX9	5.4e-48	53.95	Ferrochelatase 2 isoform 3 OS=Theobroma cacao OX=3641 GN=TCM_003334 PE=3 SV=1	[more]
A0A6J1L275	2.1e-44	42.44	Ferrochelatase OS=Cucurbita maxima OX=3661 GN=LOC111499733 PE=3 SV=1	[more]
A0A6J1G6H7	2.1e-44	42.44	Ferrochelatase OS=Cucurbita moschata OX=3662 GN=LOC111451226 PE=3 SV=1	[more]
A0A061DPW8	2.8e-44	47.31	Ferrochelatase 2 isoform 2 OS=Theobroma cacao OX=3641 GN=TCM_003334 PE=3 SV=1	[more]
A0A1S4E153	8.0e-44	42.44	Ferrochelatase OS=Cucumis melo OX=3656 GN=LOC103496275 PE=3 SV=1	[more]

BLAST of CmoCh20G003140 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010455.1	1.5e-60	94.66	Ferrochelatase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
EOX94122.1	1.1e-47	53.95	Ferrochelatase 2 isoform 3 [Theobroma cacao]	[more]
XP_011011650.1	1.4e-45	47.69	PREDICTED: ferrochelatase-2, chloroplastic-like isoform X5 [Populus euphratica]	[more]
XP_022947333.1	4.4e-44	42.44	ferrochelatase-2, chloroplastic [Cucurbita moschata] >XP_022947334.1 ferrochelat...	[more]
XP_023007154.1	4.4e-44	42.44	ferrochelatase-2, chloroplastic [Cucurbita maxima] >XP_023007155.1 ferrochelatas...	[more]

BLAST of CmoCh20G003140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30390.1	1.0e-38	37.62	ferrochelatase 2	[more]
AT2G30390.2	1.4e-37	36.45	ferrochelatase 2	[more]
AT5G26030.1	4.7e-28	30.65	ferrochelatase 1	[more]
AT5G26030.2	4.7e-28	30.65	ferrochelatase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.1400		coord: 12..65 e-value: 1.6E-5 score: 26.5 coord: 77..180 e-value: 8.7E-7 score: 30.6
None	No IPR available	PANTHER	PTHR11108:SF4	FERROCHELATASE-1, CHLOROPLASTIC/MITOCHONDRIAL	coord: 117..177
None	No IPR available	PANTHER	PTHR11108:SF4	FERROCHELATASE-1, CHLOROPLASTIC/MITOCHONDRIAL	coord: 16..56 coord: 81..116
None	No IPR available	SUPERFAMILY	53800	Chelatase	coord: 117..174
None	No IPR available	SUPERFAMILY	53800	Chelatase	coord: 16..112
IPR001015	Ferrochelatase	PFAM	PF00762	Ferrochelatase	coord: 117..167 e-value: 1.1E-10 score: 41.2
IPR001015	Ferrochelatase	PANTHER	PTHR11108	FERROCHELATASE	coord: 16..56 coord: 81..116
IPR001015	Ferrochelatase	PANTHER	PTHR11108	FERROCHELATASE	coord: 117..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh20G003140.1	CmoCh20G003140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006783	heme biosynthetic process
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0004325	ferrochelatase activity