CmoCh20G002900 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh20G002900
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	RING-type domain-containing protein
Location	Cmo_Chr20: 1398696 .. 1400407 (+)
RNA-Seq Expression	CmoCh20G002900
Synteny	CmoCh20G002900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATACGGAGCGCCATTAACAGGTTTCAGTGCTAATACTGCAGCGGCGGAGACGACTCTGCTTCCGCCGTACAATTCTGTAGCGATTGATTCGTTTCCTAAGGCGGTGGGGGCGATGCCGGCTTCGGCATCGGCGTCGACGGGTGTTGTTATGAAGTCCGATAGCGCTATATCCTATAATCTTCCGATTCCGAGAAAGCGCGGTAGGGATCATTCCACCATAAACCGATTCCTCTCATATCCTTCCTCGCATGCTCATTCTCAGAAGAATTGTGGACGTAATTTATATTTTCTTGGCGAGGATATCTCCATCCTAGTTCAACAGCAGCAATTAGATCTGGAACGATTGATATCGCAACATGTACGGTACCGAACGCTGTTTTTAATTTATTGATGTATATAAATAGGCAAAGTAGTGGTTGAGAAACGTTGTGGTGGGTTAATTACTGACGTATATACTTGTATGGAAGCAGATGGAGAAGGTGAGGATGGAAATGGAAGAAAAACGGAAGAGAGAAGCGAGAAGGATTATGGAGGCGATAGAAGTTGGAATGATGGGGATTTTAAGGAGCAAAGAGGAAGAGATCGAGAAGATGGGGAAACTAAATTGGGAACTAGAAGAACGAGTGAATAGTCTATGCATGGAGAATCAAATATGGCGTGATGTGGCTGAGACGAACGAAGCCACCGCAAATGCTCTCCGGAGCGACCTGGAACTTGTTCTGTTAGACGTGAAAAAGGAGGAGGAGGTGGAGGAGGAGGAGAAGGCAGAGTCGTGCTGCGGAAGCAACGGCGGAGATATGGAGGAGGAGGAGGAGGAGGAAGTTGAAGAGGAGAGGAGAAAAGGGAGGAAGACGAGAAGGAAGGAGAAGGAGGAGGAGGACAGACGGTGCAAGAACTGTTGGAAGGAAGAATCATGCGTGCTGCTATTGCCATGCAGGCATCTGTGTTTGTGTACTGTTTGTGGTTCATCTCTACACCATTGTCCCATCTGCAACTCTACCAATAACGCCAGTGTTCGTGTAATCATGCCTTAGATCAACAAAAAACACACACACATATTTCATTCTTCTCTTCAAATTCTTCAAATTCTCCACCCTTTTCTACTCATTTCTAATCTCTGATCAGCCTCTGTTTATCCTCTCTCATATTTGTGTCATTTTCTTACACAAATTTCTCTAATCTCTTTATGCTTTGATTGAATCCGATGATGGGTATAGTGGAGATTATGCTTTGATTGAATCCGATGATGATGGGTATAGTGGAGACATGGGGGGAGCGGACGTAGCGTATTTTAGCGAAATTCAGGCGTATGTTAGTATTAAAATTATAAAGGAAAAAAGGAACGGAGGAGGAGGTTTAAAAGCTGACACGTCGGACTCCCGGAAATATCGGTGTTGGAGTGCGGCAGTGAGGTAGGGCCCACATCCTTTTTACCTTACTCTTTTCACACGGATTCCAATCCCTAATGCTTCCAAAATGATTTATTTATTTATTTATGTATTTGACAATTTTATGGGTTACTCTAGTAATTATGGGAATCTAATTCTTAAAATAAATAAAAAGTAATGGGTATTGGTTGAGGAGTCGCGTTGGGTCAATTTTATAATTTTGTCTTTTTGAGGGGAGCGAAATGTAAATGAAAACGGAGAGTTTTTAAGTGGGGTCCATGGGAGTGGGGGATTTTTGTGTAAAAAGAGAGAGTCAGGG

mRNA sequence

ATGGGATACGGAGCGCCATTAACAGGTTTCAGTGCTAATACTGCAGCGGCGGAGACGACTCTGCTTCCGCCGTACAATTCTGTAGCGATTGATTCGTTTCCTAAGGCGGTGGGGGCGATGCCGGCTTCGGCATCGGCGTCGACGGGTGTTGTTATGAAGTCCGATAGCGCTATATCCTATAATCTTCCGATTCCGAGAAAGCGCGGTAGGGATCATTCCACCATAAACCGATTCCTCTCATATCCTTCCTCGCATGCTCATTCTCAGAAGAATTGTGGACGTAATTTATATTTTCTTGGCGAGGATATCTCCATCCTAGTTCAACAGCAGCAATTAGATCTGGAACGATTGATATCGCAACATATGGAGAAGGTGAGGATGGAAATGGAAGAAAAACGGAAGAGAGAAGCGAGAAGGATTATGGAGGCGATAGAAGTTGGAATGATGGGGATTTTAAGGAGCAAAGAGGAAGAGATCGAGAAGATGGGGAAACTAAATTGGGAACTAGAAGAACGAGTGAATAGTCTATGCATGGAGAATCAAATATGGCGTGATGTGGCTGAGACGAACGAAGCCACCGCAAATGCTCTCCGGAGCGACCTGGAACTTGTTCTGTTAGACGTGAAAAAGGAGGAGGAGGTGGAGGAGGAGGAGAAGGCAGAGTCGTGCTGCGGAAGCAACGGCGGAGATATGGAGGAGGAGGAGGAGGAGGAAGTTGAAGAGGAGAGGAGAAAAGGGAGGAAGACGAGAAGGAAGGAGAAGGAGGAGGAGGACAGACGGTGCAAGAACTGTTGGAAGGAAGAATCATGCGTGCTGCTATTGCCATGCAGGCATCTGTGTTTGTGTACTGTTTGTGGTTCATCTCTACACCATTGTCCCATCTGCAACTCTACCAATAACGCCAGTGTTCGTGTAATCATGCCTTAGATCAACAAAAAACACACACACATATTTCATTCTTCTCTTCAAATTCTTCAAATTCTCCACCCTTTTCTACTCATTTCTAATCTCTGATCAGCCTCTGTTTATCCTCTCTCATATTTGTGTCATTTTCTTACACAAATTTCTCTAATCTCTTTATGCTTTGATTGAATCCGATGATGGGTATAGTGGAGATTATGCTTTGATTGAATCCGATGATGATGGGTATAGTGGAGACATGGGGGGAGCGGACGTAGCGTATTTTAGCGAAATTCAGGCGTATGTTAGTATTAAAATTATAAAGGAAAAAAGGAACGGAGGAGGAGGTTTAAAAGCTGACACGTCGGACTCCCGGAAATATCGGTGTTGGAGTGCGGCAGTGAGGTAGGGCCCACATCCTTTTTACCTTACTCTTTTCACACGGATTCCAATCCCTAATGCTTCCAAAATGATTTATTTATTTATTTATGTATTTGACAATTTTATGGGTTACTCTAGTAATTATGGGAATCTAATTCTTAAAATAAATAAAAAGTAATGGGTATTGGTTGAGGAGTCGCGTTGGGTCAATTTTATAATTTTGTCTTTTTGAGGGGAGCGAAATGTAAATGAAAACGGAGAGTTTTTAAGTGGGGTCCATGGGAGTGGGGGATTTTTGTGTAAAAAGAGAGAGTCAGGG

Coding sequence (CDS)

ATGGGATACGGAGCGCCATTAACAGGTTTCAGTGCTAATACTGCAGCGGCGGAGACGACTCTGCTTCCGCCGTACAATTCTGTAGCGATTGATTCGTTTCCTAAGGCGGTGGGGGCGATGCCGGCTTCGGCATCGGCGTCGACGGGTGTTGTTATGAAGTCCGATAGCGCTATATCCTATAATCTTCCGATTCCGAGAAAGCGCGGTAGGGATCATTCCACCATAAACCGATTCCTCTCATATCCTTCCTCGCATGCTCATTCTCAGAAGAATTGTGGACGTAATTTATATTTTCTTGGCGAGGATATCTCCATCCTAGTTCAACAGCAGCAATTAGATCTGGAACGATTGATATCGCAACATATGGAGAAGGTGAGGATGGAAATGGAAGAAAAACGGAAGAGAGAAGCGAGAAGGATTATGGAGGCGATAGAAGTTGGAATGATGGGGATTTTAAGGAGCAAAGAGGAAGAGATCGAGAAGATGGGGAAACTAAATTGGGAACTAGAAGAACGAGTGAATAGTCTATGCATGGAGAATCAAATATGGCGTGATGTGGCTGAGACGAACGAAGCCACCGCAAATGCTCTCCGGAGCGACCTGGAACTTGTTCTGTTAGACGTGAAAAAGGAGGAGGAGGTGGAGGAGGAGGAGAAGGCAGAGTCGTGCTGCGGAAGCAACGGCGGAGATATGGAGGAGGAGGAGGAGGAGGAAGTTGAAGAGGAGAGGAGAAAAGGGAGGAAGACGAGAAGGAAGGAGAAGGAGGAGGAGGACAGACGGTGCAAGAACTGTTGGAAGGAAGAATCATGCGTGCTGCTATTGCCATGCAGGCATCTGTGTTTGTGTACTGTTTGTGGTTCATCTCTACACCATTGTCCCATCTGCAACTCTACCAATAACGCCAGTGTTCGTGTAATCATGCCTTAG

Protein sequence

MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISYNLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQHMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMENQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNNASVRVIMP

Homology

[Close]

BLAST of CmoCh20G002900 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 5.8e-52
Identity = 136/297 (45.79%), Postives = 186/297 (62.63%), Query Frame = 0


Query: 14  TAAAETTLLPP-YNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISYNLPI-PRKRGRD 73
           T   +T+L  P YN   +D         P   S  + +   SD+ + +  P+  RKR R+
Sbjct: 44  TVECQTSLFNPIYNISPVDRLVHQ-SMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSRE 103

Query: 74  HSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQHMEKVRMEMEE 133
            S +      PS++   QKN    L FLG+D+S  VQQ   D++RLIS H+E++RME+EE
Sbjct: 104 ESVVLN----PSAYMQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEE 163

Query: 134 KRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMENQIWRDVAETNE 193
           KRK + RRI+EA+E G+M  LR+K++EI  +GKLN  LEE+V SLC+ENQIWRDVA++NE
Sbjct: 164 KRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNE 223

Query: 194 ATANALRSDLELVLLDVKK---EEEVEEEEKAESCCGSNGGDMEEEEEEEVEEERRKGRK 253
           AT NALRS+L+ VL  V++   EE     + A+SCCGSN     EEE  ++  E +  +K
Sbjct: 224 ATVNALRSNLQQVLAAVERNRWEEPPTVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKK 283

Query: 254 TRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNNASVRV 306
             R         C++C K E+ VLLLPCRH+CLC+VCGSSL+ CPIC S   AS+ V
Sbjct: 284 MCRVGMS----MCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHV 331

[Close]

BLAST of CmoCh20G002900 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.2e-50
Identity = 154/329 (46.81%), Postives = 191/329 (58.05%), Query Frame = 0


Query: 2   GYGAPLTGFSANTAAAETTLLPPYNSV--AIDSFPKAVGAMPASASASTGVVMKSDSAIS 61
           GYG        N  +  TT  PP   V    D+ P   G     A  +T +  +      
Sbjct: 42  GYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYY---ADGATNLDCEF----- 101

Query: 62  YNLPIP-RKRGRDHSTIN---RFLSYPSS----HAHSQKNCGRNLYFLGEDISIL--VQQ 121
              P+P RKR RD S  N     L  P S    +A +         FLG+DI I   + Q
Sbjct: 102 --FPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQ 161

Query: 122 QQLDLERLISQH---MEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLN 181
           QQ +++R +S H   ME+V+ E+EEKRKR+AR IMEAIE G++  LR KEEE E++GK+N
Sbjct: 162 QQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVN 221

Query: 182 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVLLDVKK-------EEEVEEEEK 241
             LEERV SL +ENQIWRD+A+TNEATAN LR++LE VL  VK        E+ + EE+ 
Sbjct: 222 HALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDD 281

Query: 242 AESCCGSN-GGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRH 301
           AESCCGS+ GG  EE     V  ER    K  R+ +    R C+NC +EESCVLLLPCRH
Sbjct: 282 AESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRR----RMCRNCGEEESCVLLLPCRH 341

Query: 302 LCLCTVCGSSLHHCPICNSTNNASVRVIM 308
           LCLC VCGSS+H CPIC S  NASV V M
Sbjct: 342 LCLCGVCGSSVHTCPICTSPKNASVHVNM 356

[Close]

BLAST of CmoCh20G002900 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 5.5e-34
Identity = 92/209 (44.02%), Postives = 130/209 (62.20%), Query Frame = 0


Query: 107 VQQQQLDLERLISQHMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLN 166
           +QQQ  +++R ++Q  E +R+E+E +++ + R +  A++  ++  L++K+EEI +MGKLN
Sbjct: 106 IQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLN 165

Query: 167 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVL-----LDVKKEEEVEEEEKAE 226
           W L+ERV +L +ENQIWRD+A+TNEATAN LRS+LE VL     LD  +   VEE + AE
Sbjct: 166 WVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAE 225

Query: 227 SCCGS-NGGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLC 286
           S CGS +GGD+                          +  CK C +  + VL+LPCRHLC
Sbjct: 226 SSCGSCDGGDV----------------------TAVVNGGCKRCGELTASVLVLPCRHLC 285

Query: 287 LCTVCGSS--LHHCPICNSTNNASVRVIM 308
           LCTVCGSS  L  CP+C+    ASV V M
Sbjct: 286 LCTVCGSSALLRTCPVCDMVMTASVHVNM 292

[Close]

BLAST of CmoCh20G002900 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.5e-31
Identity = 101/264 (38.26%), Postives = 145/264 (54.92%), Query Frame = 0


Query: 53  KSDSAISYNLPIP-----RKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILV 112
           K++S +SYN  +P     R+R    S  N  +   S    S      N+    E +S + 
Sbjct: 61  KAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINV----ELVSQIQ 120

Query: 113 QQQQLDLERLISQHMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNW 172
            QQQ +++R ++Q  EK+R+E+E +++ + R +  A++  +   L+ K++EI ++  LNW
Sbjct: 121 NQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNW 180

Query: 173 ELEERVNSLCMENQIWRDVAETNEATANALRSDLELVL--LDVKKEEEVEEEEKAESCCG 232
            L+ERV SL +ENQIWRD+A+TNEA AN LR++L+ VL  L+         E+ AES CG
Sbjct: 181 VLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSCG 240

Query: 233 SNGGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVC 292
           S  GD   E    V                     CK C + E+ VL+LPCRHLCLCTVC
Sbjct: 241 SCCGDGGGEAVTAV------------------GGGCKRCGEREASVLVLPCRHLCLCTVC 300

Query: 293 GSS--LHHCPICNSTNNASVRVIM 308
           G S  L  CP+C+   NASV V M
Sbjct: 301 GGSALLRTCPVCDMVMNASVHVNM 302

[Close]

BLAST of CmoCh20G002900 vs. ExPASy TrEMBL
Match: A0A6J1FUW1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448331 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.3e-163
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240

Query: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300
           EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN
Sbjct: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300

Query: 301 ASVRVIMP 309
           ASVRVIMP
Sbjct: 301 ASVRVIMP 308

[Close]

BLAST of CmoCh20G002900 vs. ExPASy TrEMBL
Match: A0A6J1JCV5 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.5e-162
Identity = 305/308 (99.03%), Postives = 306/308 (99.35%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKA+GAMPASASASTGVVMKSDS ISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKALGAMPASASASTGVVMKSDSGISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRN YFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240

Query: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300
           EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN
Sbjct: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300

Query: 301 ASVRVIMP 309
           ASVRVIMP
Sbjct: 301 ASVRVIMP 308

[Close]

BLAST of CmoCh20G002900 vs. ExPASy TrEMBL
Match: A0A6J1FS66 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448331 PE=4 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.2e-162
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 H-MEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180
           H MEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME
Sbjct: 121 HQMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180

Query: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEV 240
           NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEV
Sbjct: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEV 240

Query: 241 EEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTN 300
           EEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTN
Sbjct: 241 EEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTN 300

Query: 301 NASVRVIMP 309
           NASVRVIMP
Sbjct: 301 NASVRVIMP 309

[Close]

BLAST of CmoCh20G002900 vs. ExPASy TrEMBL
Match: A0A6J1JF86 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.1e-161
Identity = 305/309 (98.71%), Postives = 306/309 (99.03%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKA+GAMPASASASTGVVMKSDS ISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKALGAMPASASASTGVVMKSDSGISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRN YFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 H-MEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180
           H MEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME
Sbjct: 121 HQMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180

Query: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEV 240
           NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEV
Sbjct: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEV 240

Query: 241 EEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTN 300
           EEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTN
Sbjct: 241 EEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTN 300

Query: 301 NASVRVIMP 309
           NASVRVIMP
Sbjct: 301 NASVRVIMP 309

[Close]

BLAST of CmoCh20G002900 vs. ExPASy TrEMBL
Match: A0A6J1G785 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451502 PE=4 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 8.9e-112
Identity = 239/333 (71.77%), Postives = 257/333 (77.18%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSAN AAAETTLLPPYN +AIDSFPKA  AM  +A+AST   +KSDS I+Y
Sbjct: 1   MGYGAPLTGFSANAAAAETTLLPPYNYIAIDSFPKAAAAM-TTATASTAAGLKSDSGITY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NL IPRKR RDHSTINRF SYPS  AH QKNCG N  FLGEDIS    QQQ DL+RLISQ
Sbjct: 61  NLRIPRKRARDHSTINRFASYPSP-AHFQKNCGCNHDFLGEDISDQFHQQQFDLDRLISQ 120

Query: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKR+ARRI+EAIE+GMM ILRSKEEEIEK+GKLNWELEERVNSLCME 
Sbjct: 121 HMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCMET 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEE------------VEEEEKAESCCGSNG 240
           QIWRDVA+TNEATANALRS+LE VL  V+  +                 + AESCCGSN 
Sbjct: 181 QIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAVDDAESCCGSNY 240

Query: 241 GDMEE-------------EEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLP 300
            D ++             EE EE EE  R+GRKTR   K  EDR CKNCWKEESCVLLLP
Sbjct: 241 DDDDDRKQANHGRWRNLNEEVEESEERNRRGRKTR---KGNEDRWCKNCWKEESCVLLLP 300

Query: 301 CRHLCLCTVCGSSLHHCPICNSTNNASVRVIMP 309
           CRHLCLCTVCGSSLH CPICNSTNNASVRVIMP
Sbjct: 301 CRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 328

[Close]

BLAST of CmoCh20G002900 vs. NCBI nr
Match: XP_022943619.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 585.5 bits (1508), Expect = 2.7e-163
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240

Query: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300
           EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN
Sbjct: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300

Query: 301 ASVRVIMP 309
           ASVRVIMP
Sbjct: 301 ASVRVIMP 308

[Close]

BLAST of CmoCh20G002900 vs. NCBI nr
Match: XP_022986010.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita maxima])

HSP 1 Score: 581.3 bits (1497), Expect = 5.1e-162
Identity = 305/308 (99.03%), Postives = 306/308 (99.35%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKA+GAMPASASASTGVVMKSDS ISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKALGAMPASASASTGVVMKSDSGISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRN YFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240

Query: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300
           EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN
Sbjct: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300

Query: 301 ASVRVIMP 309
           ASVRVIMP
Sbjct: 301 ASVRVIMP 308

[Close]

BLAST of CmoCh20G002900 vs. NCBI nr
Match: XP_022943616.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucurbita moschata] >XP_022943617.1 probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 580.9 bits (1496), Expect = 6.6e-162
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 H-MEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180
           H MEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME
Sbjct: 121 HQMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180

Query: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEV 240
           NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEV
Sbjct: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEV 240

Query: 241 EEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTN 300
           EEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTN
Sbjct: 241 EEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTN 300

Query: 301 NASVRVIMP 309
           NASVRVIMP
Sbjct: 301 NASVRVIMP 309

[Close]

BLAST of CmoCh20G002900 vs. NCBI nr
Match: KAG7010432.1 (putative BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 579.7 bits (1493), Expect = 1.5e-161
Identity = 305/308 (99.03%), Postives = 306/308 (99.35%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSV IDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVPIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSH HSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHDHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240

Query: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300
           EERRKGRKTRRKE+EEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN
Sbjct: 241 EERRKGRKTRRKEEEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300

Query: 301 ASVRVIMP 309
           ASVRVIMP
Sbjct: 301 ASVRVIMP 308

[Close]

BLAST of CmoCh20G002900 vs. NCBI nr
Match: KAG6570578.1 (putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 579.7 bits (1493), Expect = 1.5e-161
Identity = 305/308 (99.03%), Postives = 306/308 (99.35%), Query Frame = 0


Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSV IDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 251 MGYGAPLTGFSANTAAAETTLLPPYNSVPIDSFPKAVGAMPASASASTGVVMKSDSAISY 310

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSH HSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 311 NLPIPRKRGRDHSTINRFLSYPSSHDHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 370

Query: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 371 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 430

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE
Sbjct: 431 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVE 490

Query: 241 EERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 300
           EERRKGRKTRRKE+EEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN
Sbjct: 491 EERRKGRKTRRKEEEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNN 550

Query: 301 ASVRVIMP 309
           ASVRVIMP
Sbjct: 551 ASVRVIMP 558

[Close]

BLAST of CmoCh20G002900 vs. TAIR 10
Match: AT3G12920.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 206.1 bits (523), Expect = 4.1e-53
Identity = 136/297 (45.79%), Postives = 186/297 (62.63%), Query Frame = 0


Query: 14  TAAAETTLLPP-YNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISYNLPI-PRKRGRD 73
           T   +T+L  P YN   +D         P   S  + +   SD+ + +  P+  RKR R+
Sbjct: 44  TVECQTSLFNPIYNISPVDRLVHQ-SMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSRE 103

Query: 74  HSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQHMEKVRMEMEE 133
            S +      PS++   QKN    L FLG+D+S  VQQ   D++RLIS H+E++RME+EE
Sbjct: 104 ESVVLN----PSAYMQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEE 163

Query: 134 KRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMENQIWRDVAETNE 193
           KRK + RRI+EA+E G+M  LR+K++EI  +GKLN  LEE+V SLC+ENQIWRDVA++NE
Sbjct: 164 KRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNE 223

Query: 194 ATANALRSDLELVLLDVKK---EEEVEEEEKAESCCGSNGGDMEEEEEEEVEEERRKGRK 253
           AT NALRS+L+ VL  V++   EE     + A+SCCGSN     EEE  ++  E +  +K
Sbjct: 224 ATVNALRSNLQQVLAAVERNRWEEPPTVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKK 283

Query: 254 TRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNSTNNASVRV 306
             R         C++C K E+ VLLLPCRH+CLC+VCGSSL+ CPIC S   AS+ V
Sbjct: 284 MCRVGMS----MCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHV 331

[Close]

BLAST of CmoCh20G002900 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 205.7 bits (522), Expect = 5.4e-53
Identity = 154/326 (47.24%), Postives = 191/326 (58.59%), Query Frame = 0


Query: 2   GYGAPLTGFSANTAAAETTLLPPYNSV--AIDSFPKAVGAMPASASASTGVVMKSDSAIS 61
           GYG        N  +  TT  PP   V    D+ P   G     A  +T +  +      
Sbjct: 42  GYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYY---ADGATNLDCEF----- 101

Query: 62  YNLPIP-RKRGRDHSTIN---RFLSYPSS----HAHSQKNCGRNLYFLGEDISIL--VQQ 121
              P+P RKR RD S  N     L  P S    +A +         FLG+DI I   + Q
Sbjct: 102 --FPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQ 161

Query: 122 QQLDLERLISQHMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWEL 181
           QQ +++R +S HME+V+ E+EEKRKR+AR IMEAIE G++  LR KEEE E++GK+N  L
Sbjct: 162 QQHEIDRFVSLHMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHAL 221

Query: 182 EERVNSLCMENQIWRDVAETNEATANALRSDLELVLLDVKK-------EEEVEEEEKAES 241
           EERV SL +ENQIWRD+A+TNEATAN LR++LE VL  VK        E+ + EE+ AES
Sbjct: 222 EERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAES 281

Query: 242 CCGSN-GGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCL 301
           CCGS+ GG  EE     V  ER    K  R+ +    R C+NC +EESCVLLLPCRHLCL
Sbjct: 282 CCGSSCGGGGEETVRRRVGLEREAQDKAERRRR----RMCRNCGEEESCVLLLPCRHLCL 341

Query: 302 CTVCGSSLHHCPICNSTNNASVRVIM 308
           C VCGSS+H CPIC S  NASV V M
Sbjct: 342 CGVCGSSVHTCPICTSPKNASVHVNM 353

[Close]

BLAST of CmoCh20G002900 vs. TAIR 10
Match: AT1G79110.1 (zinc ion binding )

HSP 1 Score: 200.3 bits (508), Expect = 2.3e-51
Identity = 154/329 (46.81%), Postives = 191/329 (58.05%), Query Frame = 0


Query: 2   GYGAPLTGFSANTAAAETTLLPPYNSV--AIDSFPKAVGAMPASASASTGVVMKSDSAIS 61
           GYG        N  +  TT  PP   V    D+ P   G     A  +T +  +      
Sbjct: 42  GYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYY---ADGATNLDCEF----- 101

Query: 62  YNLPIP-RKRGRDHSTIN---RFLSYPSS----HAHSQKNCGRNLYFLGEDISIL--VQQ 121
              P+P RKR RD S  N     L  P S    +A +         FLG+DI I   + Q
Sbjct: 102 --FPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQ 161

Query: 122 QQLDLERLISQH---MEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLN 181
           QQ +++R +S H   ME+V+ E+EEKRKR+AR IMEAIE G++  LR KEEE E++GK+N
Sbjct: 162 QQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVN 221

Query: 182 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVLLDVKK-------EEEVEEEEK 241
             LEERV SL +ENQIWRD+A+TNEATAN LR++LE VL  VK        E+ + EE+ 
Sbjct: 222 HALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDD 281

Query: 242 AESCCGSN-GGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRH 301
           AESCCGS+ GG  EE     V  ER    K  R+ +    R C+NC +EESCVLLLPCRH
Sbjct: 282 AESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRR----RMCRNCGEEESCVLLLPCRH 341

Query: 302 LCLCTVCGSSLHHCPICNSTNNASVRVIM 308
           LCLC VCGSS+H CPIC S  NASV V M
Sbjct: 342 LCLCGVCGSSVHTCPICTSPKNASVHVNM 356

[Close]

BLAST of CmoCh20G002900 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 146.4 bits (368), Expect = 3.9e-35
Identity = 92/209 (44.02%), Postives = 130/209 (62.20%), Query Frame = 0


Query: 107 VQQQQLDLERLISQHMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLN 166
           +QQQ  +++R ++Q  E +R+E+E +++ + R +  A++  ++  L++K+EEI +MGKLN
Sbjct: 106 IQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLN 165

Query: 167 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVL-----LDVKKEEEVEEEEKAE 226
           W L+ERV +L +ENQIWRD+A+TNEATAN LRS+LE VL     LD  +   VEE + AE
Sbjct: 166 WVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAE 225

Query: 227 SCCGS-NGGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLC 286
           S CGS +GGD+                          +  CK C +  + VL+LPCRHLC
Sbjct: 226 SSCGSCDGGDV----------------------TAVVNGGCKRCGELTASVLVLPCRHLC 285

Query: 287 LCTVCGSS--LHHCPICNSTNNASVRVIM 308
           LCTVCGSS  L  CP+C+    ASV V M
Sbjct: 286 LCTVCGSSALLRTCPVCDMVMTASVHVNM 292

[Close]

BLAST of CmoCh20G002900 vs. TAIR 10
Match: AT5G45100.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 146.4 bits (368), Expect = 3.9e-35
Identity = 92/209 (44.02%), Postives = 130/209 (62.20%), Query Frame = 0


Query: 107 VQQQQLDLERLISQHMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLN 166
           +QQQ  +++R ++Q  E +R+E+E +++ + R +  A++  ++  L++K+EEI +MGKLN
Sbjct: 79  IQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLN 138

Query: 167 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVL-----LDVKKEEEVEEEEKAE 226
           W L+ERV +L +ENQIWRD+A+TNEATAN LRS+LE VL     LD  +   VEE + AE
Sbjct: 139 WVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAE 198

Query: 227 SCCGS-NGGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLC 286
           S CGS +GGD+                          +  CK C +  + VL+LPCRHLC
Sbjct: 199 SSCGSCDGGDV----------------------TAVVNGGCKRCGELTASVLVLPCRHLC 258

Query: 287 LCTVCGSS--LHHCPICNSTNNASVRVIM 308
           LCTVCGSS  L  CP+C+    ASV V M
Sbjct: 259 LCTVCGSSALLRTCPVCDMVMTASVHVNM 265

The following BLAST results are available for this feature:

BLAST of CmoCh20G002900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDD1	5.8e-52	45.79	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]
F4IDI6	3.2e-50	46.81	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
Q9FHE4	5.5e-34	44.02	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
O81851	2.5e-31	38.26	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]

BLAST of CmoCh20G002900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FUW1	1.3e-163	100.00	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita mosch...	[more]
A0A6J1JCV5	2.5e-162	99.03	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxim...	[more]
A0A6J1FS66	3.2e-162	99.68	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 OS=Cucurbita mosch...	[more]
A0A6J1JF86	6.1e-161	98.71	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxim...	[more]
A0A6J1G785	8.9e-112	71.77	probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662...	[more]

BLAST of CmoCh20G002900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943619.1	2.7e-163	100.00	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita moschat...	[more]
XP_022986010.1	5.1e-162	99.03	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita maxima]	[more]
XP_022943616.1	6.6e-162	99.68	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucurbita moschat...	[more]
KAG7010432.1	1.5e-161	99.03	putative BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita argyrosperma subsp...	[more]
KAG6570578.1	1.5e-161	99.03	putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrospe...	[more]

BLAST of CmoCh20G002900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12920.1	4.1e-53	45.79	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G79110.2	5.4e-53	47.24	zinc ion binding	[more]
AT1G79110.1	2.3e-51	46.81	zinc ion binding	[more]
AT5G45100.1	3.9e-35	44.02	SBP (S-ribonuclease binding protein) family protein	[more]
AT5G45100.2	3.9e-35	44.02	SBP (S-ribonuclease binding protein) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 231..256
None	No IPR available	COILS	Coil	Coil	coord: 152..172
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 257..297 e-value: 8.0E-8 score: 32.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 214..250
None	No IPR available	PANTHER	PTHR42647:SF12	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATED	coord: 15..307
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 230..308 e-value: 7.6E-13 score: 49.7
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 15..307
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 261..296 score: 8.919052

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh20G002900.1	CmoCh20G002900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0043067	regulation of programmed cell death
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity