CmoCh20G001920 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G001920
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionFatty acyl-CoA reductase
LocationCmo_Chr20: 946823 .. 949996 (+)
RNA-Seq ExpressionCmoCh20G001920
SyntenyCmoCh20G001920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGGTAAGTCTTGCCTAAATCAGGGAGTAGTTAATTTAAATTGATTAGAGATGAGGTGATAACCTATCATTCTATAATTTCTCTAAATGCTTAATTAGGCAATAAAAGGAAAGTTAATTACTTGAGTTAGGTGCATTACTACTCCTTTTAATCTATTTTGGTATATAATGCAAACTTCATGTTGATTTCTTCACTTTAATGTATATAATCTTCATGGACTTTTTAGAGAATAAGACCATTTTTGTGCTGGGTGCCAAAGGGTTTCTTGGAAAGAGTATGGGAATTTTTTTCATTAATTTGTGTTATTATGTTAAATATATAATTTATATTATATGGTTTGAGTGTGCAGTTATGGTGGAGAAACTATTAAGAACTCATCCCAACCTGAAGAAACTTTATTTACTTTTACGGAACGTGGATCAAATCACAGCTTCAAAACATTTCTGTGATGAGGTATATGACTATCAAAACACTAACTAGCTAGTTTATGTTAATACTAATCATAAAAGGTTTGAATCCTTCACCCATAATCGTTATTTCGGTAAAAAGTTATAATATTACCTTATTGATGGTTTGATTTATAGGTTGTCGAGAAGGAATTGTTTAAAGTGTTAAGCGAAAAATGGGGTGGAGATCTAAAAACCCTTGTCTCGGAGAAGATTTGTTTGGTGTCTGGAGATATTTCATCCCCAAACATGGGATTAAAGGACTCAAATTTATTGGAAGAGATGAAGAATCGAGTGGAAATAATCATTAATTTTGCTGCATCAACGAATTTTGATGAAAGGTACCGACTCTCTCGAGTTGTGTTGATCAGAAAGTATTCCAAATTGATCCTAAGTTTGTTTACTTTCTTGCAGATATGATGTAGCATTTGGTACCAATGTATTGGGAGCTAAACATGTTTTAAACTTTGCAAAGCAATGTTCTAATTTCGAAATTCTTGTCCACGTATCCACTGGTTCGTATCCAATCATATTATTTTTTTATTTAAGTAATTTAGTTAATTACCCACAAATTGATTGAGACAGATGTTTTGTCAGCTTTTGTTTCGAATCAAAGGGAAGGAGTTATCTTGGAAACTCCGTGCAAATTGGACGAGTCTGTTGATGGCACTTCGAAGTTAAATATTGAATCAGAACGGAAAATTATTGAAGAGAGTTTAAGAGAATTGAGAAATAATCGAGATATAAATAAAATTATGAGGAGTTTGGTCATGAAAGATTTGGGCACAAAAAGGTATGCAAGATCAATAGATATCATCCTCTTCGTCAACGTAACAATTTTATTCTTTTATTGTTATTAGGGCAAAAATGTACGGATATCCAAACACGTACGCATTTACGAAAGCAATGGGCGAGATGTTGATGAATGATAGAATTGACAATCTTCCTTTGATCATCATGCGACCAACCATAGTCACAAGCACTTACAAAGAACCTTTTCCCGGTTGGATCGAAGGTTTGAGGACCATTGATGGCTTTATTGTTGGGTATGGGAAAGGAAAAGTAACACGCTTTCCTTCCGATGTTAGCTCAATCTTAGACTTGGTGAGATGAATATAATTTCTTCCAAATTTTAATCTCTCCTTGTAAATCTAAGAAGTTCGATGTTCTAAAGAAATGCAGGTTCCAGCCGACATGGTAGTAAATGCTATCATTATGGCAATACTGACACATAAACTTCAACCATCTGGACGTACAATTATATATCACATAGGTTCTTCAATGAGAAATTCAATGAGGATCGACACTTTGTTCAGTTATATTCACCAATACTTTACGAAAAAACCATGGATGAATGAAGATGGAAAGGTCATCAAGGTTAAGAAAATTACCTCATTCAACAACATTGCTAGCTTCCATCGATACATGACCATTCACTACGTCTTTCTCAAGGTATGTTCCAAAATCTATAACTAATACTTACACGAAACTATATTTCAATTTATATTTTCTAATCGGTATTGTTGTTACTGTAAAGGGTTTTGAGTTTGTGAACAAAGCATTTTGCTACTCTTTCCAAGACAAGTATAATGATCTTCAAAGAAAGTTCAACTGGGTGATGCGACAAGTTGAGCTTTACAACTCGTTTATATTCTTCAAAGCCAAGTAATTTCTAAACTAATTTTATACGAAACATTGAACTTAGTATATATACCACGTTCTAAAATAATCAGATTTGTGTGATTTTTATATCTACCGAACATATGAATACATGTCAAAGGTTAATAAGTCAGAATTTATATAATCTGAGTTTACGAGACTTTGTATAACTTTATTTTTGTTTGTACAGATTTGACGACACCAACTTAAAAAAACTGAGAATTGTTGCACGAGACAACGGCATAAATCCAAATACATCGCTTTTGGATCCAAAGGATATAAACTGGGAAGATTACTTTCTGAATATCCACGTTCCTGGACTTGTTAAATATGTAATGAAATGATTAAAATATCATTTCTTTATGTGTACTCAAAAGTAGCCTAAAATAATTACAATTTCAAGAACTTCAATCCCAAAAACATATCAGCTCAACATATCAGCTCTTTGACATCCGTGATTCAAGGAAATATCTTAATTAGCTGCCTATGATATAGTCCAAAGCTGAACCAGTATTGCAAGAAATGTTAATGATAATAGGTCCTTAATTTCAACACACTGCACATGGATATCTTTCTATTGTCAAACGGAAGTGAATCTTTCCTTTTAAAGTGTAACATCCCCACGTTCTAGGAATTCTTATGCAACAGTTTAAGCCTACCGCTAGCAGATATTGTCTACTTTAGCTCATTACCTATCACTGTCAGCCTCACAATTTTAAAATGCATCTATGAGGAATTTTTATGAGAACATTTTATGCAAAATATTTTTTCTATATTCACATGAAAGTGATGTTCGCACATCACAAATTTTATTTTTCGGGGTCCTAAACAAAGCTTAAACGTTTTTATGTTCTAACTATTTCGAGACAATCAAGTTCGGAAAGAAACTTTTGGAGCATCTTAGAAGTTCATTAATTGGAGCAGCAACCAAAAAGTTTGACGATGAGCTGCTTGCTGTAGTGAACCTCCAATTTATTACATCCTTCAATTTTTCATACGAGTATGTCAACAGTTAGTTGTAAGTTTTAATGGTTTTCAGTCTTCAACTACAGTTCCGTAATCTAA

mRNA sequence

ATGGCAGTTATGGTGGAGAAACTATTAAGAACTCATCCCAACCTGAAGAAACTTTATTTACTTTTACGGAACGTGGATCAAATCACAGCTTCAAAACATTTCTGTGATGAGGTTGTCGAGAAGGAATTGTTTAAAGTGTTAAGCGAAAAATGGGGTGGAGATCTAAAAACCCTTGTCTCGGAGAAGATTTGTTTGGTGTCTGGAGATATTTCATCCCCAAACATGGGATTAAAGGACTCAAATTTATTGGAAGAGATGAAGAATCGAGTGGAAATAATCATTAATTTTGCTGCATCAACGAATTTTGATGAAAGGTACCGACTCTCTCGAGTTGTGTTGATCAGAAAATATGATGTAGCATTTGGTACCAATGTATTGGGAGCTAAACATGTTTTAAACTTTGCAAAGCAATGTTCTAATTTCGAAATTCTTGTCCACTTAATTACCCACAAATTGATTGAGACAGATGTTTTGTCAGCTTTTGTTTCGAATCAAAGGGAAGGAGTTATCTTGGAAACTCCGTGCAAATTGGACGAGTCTGTTGATGGCACTTCGAAGTTAAATATTGAATCAGAACGGAAAATTATTGAAGAGAGTTTAAGAGAATTGAGAAATAATCGAGATATAAATAAAATTATGAGGAGTTTGGTCATGAAAGATTTGGGCACAAAAAGGGCAAAAATGTACGGATATCCAAACACGTACGCATTTACGAAAGCAATGGGCGAGATGTTGATGAATGATAGAATTGACAATCTTCCTTTGATCATCATGCGACCAACCATAGTCACAAGCACTTACAAAGAACCTTTTCCCGGTTGGATCGAAGGTTTGAGGACCATTGATGGCTTTATTGTTGGGTATGGGAAAGGAAAAGTAACACGCTTTCCTTCCGATGTTCCAGCCGACATGGTAGTAAATGCTATCATTATGGCAATACTGACACATAAACTTCAACCATCTGGACGTACAATTATATATCACATAGGTTCTTCAATGAGAAATTCAATGAGGATCGACACTTTGTTCAGTTATATTCACCAATACTTTACGAAAAAACCATGGATGAATGAAGATGGAAAGGTCATCAAGGTTAAGAAAATTACCTCATTCAACAACATTGCTAGCTTCCATCGATACATGACCATTCACTACGTCTTTCTCAAGGGTTTTGAGTTTGTGAACAAAGCATTTTGCTACTCTTTCCAAGACAAGTATAATGATCTTCAAAGAAAGTTCAACTGGGTGATGCGACAAGTTGAGCTTTACAACTCGTTTATATTCTTCAAAGCCAAATTTGACGACACCAACTTAAAAAAACTGAGAATTGTTGCACGAGACAACGGCATAAATCCAAATACATCGCTTTTGGATCCAAAGGATATAAACTGGGAAGATTACTTTCTGAATATCCACGTTCCTGGACTTGTTAAATATACAATCAAGTTCGGAAAGAAACTTTTGGAGCATCTTAGAAGTTCATTAATTGGAGCAGCAACCAAAAAGTTTGACGATGAGCTGCTTGCTGTAGTGAACCTCCAATTTATTACATCCTTCAATTTTTCATACGAGTATGTCAACATCTTCAACTACAGTTCCGTAATCTAA

Coding sequence (CDS)

ATGGCAGTTATGGTGGAGAAACTATTAAGAACTCATCCCAACCTGAAGAAACTTTATTTACTTTTACGGAACGTGGATCAAATCACAGCTTCAAAACATTTCTGTGATGAGGTTGTCGAGAAGGAATTGTTTAAAGTGTTAAGCGAAAAATGGGGTGGAGATCTAAAAACCCTTGTCTCGGAGAAGATTTGTTTGGTGTCTGGAGATATTTCATCCCCAAACATGGGATTAAAGGACTCAAATTTATTGGAAGAGATGAAGAATCGAGTGGAAATAATCATTAATTTTGCTGCATCAACGAATTTTGATGAAAGGTACCGACTCTCTCGAGTTGTGTTGATCAGAAAATATGATGTAGCATTTGGTACCAATGTATTGGGAGCTAAACATGTTTTAAACTTTGCAAAGCAATGTTCTAATTTCGAAATTCTTGTCCACTTAATTACCCACAAATTGATTGAGACAGATGTTTTGTCAGCTTTTGTTTCGAATCAAAGGGAAGGAGTTATCTTGGAAACTCCGTGCAAATTGGACGAGTCTGTTGATGGCACTTCGAAGTTAAATATTGAATCAGAACGGAAAATTATTGAAGAGAGTTTAAGAGAATTGAGAAATAATCGAGATATAAATAAAATTATGAGGAGTTTGGTCATGAAAGATTTGGGCACAAAAAGGGCAAAAATGTACGGATATCCAAACACGTACGCATTTACGAAAGCAATGGGCGAGATGTTGATGAATGATAGAATTGACAATCTTCCTTTGATCATCATGCGACCAACCATAGTCACAAGCACTTACAAAGAACCTTTTCCCGGTTGGATCGAAGGTTTGAGGACCATTGATGGCTTTATTGTTGGGTATGGGAAAGGAAAAGTAACACGCTTTCCTTCCGATGTTCCAGCCGACATGGTAGTAAATGCTATCATTATGGCAATACTGACACATAAACTTCAACCATCTGGACGTACAATTATATATCACATAGGTTCTTCAATGAGAAATTCAATGAGGATCGACACTTTGTTCAGTTATATTCACCAATACTTTACGAAAAAACCATGGATGAATGAAGATGGAAAGGTCATCAAGGTTAAGAAAATTACCTCATTCAACAACATTGCTAGCTTCCATCGATACATGACCATTCACTACGTCTTTCTCAAGGGTTTTGAGTTTGTGAACAAAGCATTTTGCTACTCTTTCCAAGACAAGTATAATGATCTTCAAAGAAAGTTCAACTGGGTGATGCGACAAGTTGAGCTTTACAACTCGTTTATATTCTTCAAAGCCAAATTTGACGACACCAACTTAAAAAAACTGAGAATTGTTGCACGAGACAACGGCATAAATCCAAATACATCGCTTTTGGATCCAAAGGATATAAACTGGGAAGATTACTTTCTGAATATCCACGTTCCTGGACTTGTTAAATATACAATCAAGTTCGGAAAGAAACTTTTGGAGCATCTTAGAAGTTCATTAATTGGAGCAGCAACCAAAAAGTTTGACGATGAGCTGCTTGCTGTAGTGAACCTCCAATTTATTACATCCTTCAATTTTTCATACGAGTATGTCAACATCTTCAACTACAGTTCCGTAATCTAA

Protein sequence

MAVMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFGTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSDVPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFLKGFEFVNKAFCYSFQDKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVPGLVKYTIKFGKKLLEHLRSSLIGAATKKFDDELLAVVNLQFITSFNFSYEYVNIFNYSSVI
Homology
BLAST of CmoCh20G001920 vs. ExPASy Swiss-Prot
Match: Q93ZB9 (Fatty acyl-CoA reductase 3 OS=Arabidopsis thaliana OX=3702 GN=FAR3 PE=1 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.1e-119
Identity = 226/485 (46.60%), Postives = 322/485 (66.39%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           + VEK+LR  PN+KKLYLLLR     +A++ F DE+++K+LFKVL EK+G +L  L SEK
Sbjct: 31  IFVEKILRVAPNVKKLYLLLRASKGKSATQRFNDEILKKDLFKVLKEKYGPNLNQLTSEK 90

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I +V GDI   ++GL+D +L  EM ++V+ I+N AA+T FDER           YDVA G
Sbjct: 91  ITIVDGDICLEDLGLQDFDLAHEMIHQVDAIVNLAATTKFDER-----------YDVALG 150

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVD 182
            N LGA +VLNFAK+C+  +ILVH+ T          A+V  ++ G+I+ETP ++ E+++
Sbjct: 151 INTLGALNVLNFAKRCAKVKILVHVST----------AYVCGEKSGLIMETPYRMGETLN 210

Query: 183 GTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMG 242
           GT+ L+I  E+K+++E L +LR      + +    MKDLG +RAKMYG+PNTY FTKAMG
Sbjct: 211 GTTGLDINYEKKLVQEKLDQLRVIGAAPETITE-TMKDLGLRRAKMYGWPNTYVFTKAMG 270

Query: 243 EMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD--- 302
           EM++  + +NL L+++RP+I+TST+KEPFPGW EG+RTID   VGYGKGK+T F  D   
Sbjct: 271 EMMVGTKRENLSLVLLRPSIITSTFKEPFPGWTEGIRTIDSLAVGYGKGKLTCFLCDLDA 330

Query: 303 ----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPW 362
               +PADMVVN+I++++           IIYH+GSS+RN M+        ++YF+ KPW
Sbjct: 331 VSDVMPADMVVNSILVSMAAQ--AGKQEEIIYHVGSSLRNPMKNSKFPELAYRYFSIKPW 390

Query: 363 MNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQDKYNDLQRKFN 422
            N++GKV+KV  I   +++ SFHRYMTI Y + LKG E VN   C  F+ ++    +K N
Sbjct: 391 TNKEGKVVKVGAIEILSSMRSFHRYMTIRYLIALKGLELVNIILCKLFEKEFQYFNKKIN 450

Query: 423 WVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVP 480
           ++ R V+LY  ++FF   FDD+N +KLR +    G+       DPK ++W+DYFLN HV 
Sbjct: 451 FIFRLVDLYQPYLFFYGIFDDSNTEKLRKMVSKTGVENEMFYFDPKVLDWDDYFLNTHVI 491

BLAST of CmoCh20G001920 vs. ExPASy Swiss-Prot
Match: Q9XGY7 (Alcohol-forming fatty acyl-CoA reductase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 4.3e-111
Identity = 212/487 (43.53%), Postives = 311/487 (63.86%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           + VEK+LR+ PN+KKLYLLLR  D  TA+    +EV  KELFKVL +  G +  + VSEK
Sbjct: 28  IFVEKVLRSQPNVKKLYLLLRATDDETAALRLQNEVFGKELFKVLKQNLGANFYSFVSEK 87

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           + +V GDI+  ++ LKD NL EEM   +++++N AA+ NF ER           YDV+  
Sbjct: 88  VTVVPGDITGEDLCLKDVNLKEEMWREIDVVVNLAATINFIER-----------YDVSLL 147

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVD 182
            N  GAK+VL+FAK+C+  +I VH+ T          A+VS ++ G+ILE P  + ES++
Sbjct: 148 INTYGAKYVLDFAKKCNKLKIFVHVST----------AYVSGEKNGLILEKPYYMGESLN 207

Query: 183 GTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMG 242
           G   L+I  E+K++E  + EL+      K ++S  MKD+G +RA+ +G+PN Y FTKA+G
Sbjct: 208 GRLGLDINVEKKLVEAKINELQAAGATEKSIKS-TMKDMGIERARHWGWPNVYVFTKALG 267

Query: 243 EMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRF---PSD 302
           EML+     ++PL I+RPTI+TST+KEPFPGW+EG+RTID   V YGKG++      PS 
Sbjct: 268 EMLLMQYKGDIPLTIIRPTIITSTFKEPFPGWVEGVRTIDNVPVYYGKGRLRCMLCGPST 327

Query: 303 ----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPW 362
               +PADMVVNA I+A++ H  Q     + YH+GSS  N M++  L    H+YFTK PW
Sbjct: 328 IIDLIPADMVVNATIVAMVAHANQRYVEPVTYHVGSSAANPMKLSALPEMAHRYFTKNPW 387

Query: 363 MNEDGKVIKVKKITSFNNIASFHRYMTIHYVF-LKGFEFVNKAFCYSFQDKYNDLQRKFN 422
           +N D   + V +   F++ ++FH Y+T++++  LK  E  N  FC  F+ KY DL+RK  
Sbjct: 388 INPDRNPVHVGRAMVFSSFSTFHLYLTLNFLLPLKVLEIANTIFCQWFKGKYMDLKRKTR 447

Query: 423 WVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVP 482
            ++R V++Y  ++FF+  FDD N +KLRI A+++ +  +    DP+ INWEDYFL  H P
Sbjct: 448 LLLRLVDIYKPYLFFQGIFDDMNTEKLRIAAKESIVEADMFYFDPRAINWEDYFLKTHFP 492

BLAST of CmoCh20G001920 vs. ExPASy Swiss-Prot
Match: Q39152 (Fatty acyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=FAR1 PE=1 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 5.5e-106
Identity = 213/490 (43.47%), Postives = 305/490 (62.24%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGD-LKTLVSE 62
           V+VEK+LR  PN+KK+YLLLR  D+ +A +    EV+E +LFKVL    G D L  L+ E
Sbjct: 26  VLVEKILRLQPNVKKIYLLLRAPDEKSAMQRLRSEVMEIDLFKVLRNNLGEDNLNALMRE 85

Query: 63  KICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAF 122
           KI  V GDIS  N+GLKD++L++ M + ++IIIN AA+TNFDER           YD+  
Sbjct: 86  KIVPVPGDISIDNLGLKDTDLIQRMWSEIDIIINIAATTNFDER-----------YDIGL 145

Query: 123 GTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESV 182
           G N  GA +VLNFAK+C   ++L+H+ T          A++S ++ G++LE P K+ E++
Sbjct: 146 GINTFGALNVLNFAKKCVKGQLLLHVST----------AYISGEQPGLLLEKPFKMGETL 205

Query: 183 DGTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAM 242
            G  +L+I  E  ++++ L+EL   +D +    S  MKD G  RAK++G+PNTY FTKAM
Sbjct: 206 SGDRELDINIEHDLMKQKLKEL---QDCSDEEISQTMKDFGMARAKLHGWPNTYVFTKAM 265

Query: 243 GEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD-- 302
           GEMLM    +NLPL+I+RPT++TST  EPFPGWIEGL+T+D  IV YGKG++  F +D  
Sbjct: 266 GEMLMGKYRENLPLVIIRPTMITSTIAEPFPGWIEGLKTLDSVIVAYGKGRLKCFLADSN 325

Query: 303 -----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKP 362
                +PADMVVNA++ A   H    +G   IYH+GSS +N +    L  +  +YF K+P
Sbjct: 326 SVFDLIPADMVVNAMVAAATAHS-GDTGIQAIYHVGSSCKNPVTFGQLHDFTARYFAKRP 385

Query: 363 WMNEDGKVIKVKKITSFNNIASFHRYMTIHYVF-LKGFEFVNKAFCYSFQDKYNDLQRKF 422
            +  +G  I V K T  + +A F  YMT+ Y   L+    +N  + +S  D Y+DL RK 
Sbjct: 386 LIGRNGSPIIVVKGTILSTMAQFSLYMTLRYKLPLQILRLINIVYPWSHGDNYSDLSRKI 445

Query: 423 NWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSL-LDPKDINWEDYFLNIH 482
              MR VELY  ++ FK  FDD N ++LR+  ++N    + S   DPK I+W++Y  N H
Sbjct: 446 KLAMRLVELYQPYLLFKGIFDDLNTERLRMKRKENIKELDGSFEFDPKSIDWDNYITNTH 490

BLAST of CmoCh20G001920 vs. ExPASy Swiss-Prot
Match: Q9LXN3 (Probable fatty acyl-CoA reductase 4 OS=Arabidopsis thaliana OX=3702 GN=FAR4 PE=1 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 9.6e-103
Identity = 209/493 (42.39%), Postives = 303/493 (61.46%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGD-LKTLVSE 62
           V VEK+LR  P +KKL+LLLR  D  +A + F  EV+EK+LF+VL    G + LK  ++E
Sbjct: 26  VFVEKILRIQPKVKKLFLLLRAADNESAMQRFHSEVLEKDLFRVLKNALGDENLKAFITE 85

Query: 63  KICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAF 122
           K+  + GDIS  N+G+K S+LL+ M N ++II+N AA+TNFDER           YDV  
Sbjct: 86  KVVPIPGDISVDNLGVKGSDLLQHMWNEIDIIVNVAATTNFDER-----------YDVGL 145

Query: 123 GTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESV 182
             N  G  +VLNFAK+C   ++L+H+ T          A+V  ++ G++ E    + E++
Sbjct: 146 SVNTFGPLNVLNFAKKCVKGQLLLHVST----------AYVRGEKSGLLHEKTFHMGETL 205

Query: 183 DGTSKLNIESERKIIEESLREL-RNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKA 242
           +G  KL IE+E +++++ L+EL + N    +I +S  MKDLG  RAK++G+PNTY FTK+
Sbjct: 206 NGHRKLVIETEMELMKQKLKELQKQNCSEEEISQS--MKDLGMSRAKLHGWPNTYVFTKS 265

Query: 243 MGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD- 302
           MGEML+ +  +NLP++I+RPT++TST+ EPFPGWIEGLRTID  IV YGKG++  F +D 
Sbjct: 266 MGEMLLGNYRENLPIVIIRPTMITSTFSEPFPGWIEGLRTIDSVIVAYGKGRLKCFLADP 325

Query: 303 ------VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKK 362
                 +P DMV NA++ A   H  +   +T +YH+GSS +N +  + +      YFTK 
Sbjct: 326 NSVLDLIPVDMVANAMVTAAAIHAGKLGSQT-VYHVGSSCKNPITFEQIHDLAASYFTKN 385

Query: 363 PWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVF-LKGFEFVNKAFCYSFQDKYNDLQRK 422
           P +  DG  I V K T  + +A F  YMT+ Y   L+    +   + +   +KY D+ RK
Sbjct: 386 PLVRRDGSSILVSKGTILSTMAQFSFYMTLRYKLPLQMLRLIYVIYPWWNGNKYKDIDRK 445

Query: 423 FNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLL---DPKDINWEDYFL 482
               MR V+LY  ++ FK  FDDTN +KLR+  ++  IN     L   DPK I+WEDY  
Sbjct: 446 IKLAMRLVDLYRPYVLFKGIFDDTNTEKLRLKRKE--INKEMYGLFEFDPKSIDWEDYMT 492

BLAST of CmoCh20G001920 vs. ExPASy Swiss-Prot
Match: Q0WRB0 (Probable fatty acyl-CoA reductase 5 OS=Arabidopsis thaliana OX=3702 GN=FAR5 PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 6.9e-101
Identity = 212/493 (43.00%), Postives = 298/493 (60.45%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGD-LKTLVSE 62
           V VEK+LR  PN+KKLYLL+R  D   A+K    EV EKELFKVL +  G + L TL+ E
Sbjct: 26  VFVEKILRVQPNVKKLYLLVRASDNEAATKRLRTEVFEKELFKVLRQNLGDEKLNTLLYE 85

Query: 63  KICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAF 122
           K+  V GDI++  +G+ DS+L E M+  ++I++N AA+TNFDER           YDV  
Sbjct: 86  KVVSVPGDIATDQLGINDSHLRERMQKEIDIVVNVAATTNFDER-----------YDVGL 145

Query: 123 GTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDE-S 182
           G N  GA +VLNFAK+C   ++L+H+ T          A+V  ++ G+I E P  ++E  
Sbjct: 146 GINTFGALNVLNFAKKCVKVQLLLHVST----------AYVCGEKPGLIPEKPFIMEEIR 205

Query: 183 VDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKA 242
            +   +L+I  ER+++++ L+EL N +D ++   +L MK+LG +RAK++G+PNTY FTK+
Sbjct: 206 NENGLQLDINLERELMKQRLKEL-NEQDCSEEDITLSMKELGMERAKLHGWPNTYVFTKS 265

Query: 243 MGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSDV 302
           MGEML+    +NLPL+I+RPT++TST  EPFPGWIEGLRT+D  I+ YGKG +  F  DV
Sbjct: 266 MGEMLLGKHKENLPLVIIRPTMITSTLSEPFPGWIEGLRTVDSVIIAYGKGVLKCFLVDV 325

Query: 303 -------PADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKK 362
                  P DMV NA+I A   H    SG  ++YH+GSS +N +    +     +YFTK 
Sbjct: 326 NSVCDMIPVDMVANAMITAAAKH-AGGSGVHMVYHVGSSHQNPVTFGEIHEIAVRYFTKN 385

Query: 363 PWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVF-LKGFEFVNKAFCYSFQDKYNDLQRK 422
           P  + +G +I V K+     +A F  YMT+ Y   L+  + V+  + +   DKY D  RK
Sbjct: 386 PLRSRNGSLITVSKVRFIPTMALFSLYMTLRYKLPLQLLKLVDIIYPWRNGDKYGDKNRK 445

Query: 423 FNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLL---DPKDINWEDYFL 482
              VMR VELY  ++ FK  FDD N K L    ++  I     L+   DPK INW DY  
Sbjct: 446 IELVMRLVELYEPYVLFKGIFDDRNTKSLCANQKEEEIKNTEKLMFDFDPKGINWGDYLT 495

BLAST of CmoCh20G001920 vs. ExPASy TrEMBL
Match: A0A6J1FYH0 (Fatty acyl-CoA reductase OS=Cucurbita moschata OX=3662 GN=LOC111448658 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.3e-234
Identity = 420/454 (92.51%), Postives = 422/454 (92.95%), Query Frame = 0

Query: 38  VVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFA 97
           VVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFA
Sbjct: 485 VVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFA 544

Query: 98  ASTNFDERYRLSRVVLIRKYDVAFGTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDV 157
           ASTNFDER           YDVAFGTNVLGAKHVLNFAKQCSNFEILVH+ T        
Sbjct: 545 ASTNFDER-----------YDVAFGTNVLGAKHVLNFAKQCSNFEILVHVST-------- 604

Query: 158 LSAFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLV 217
             AFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLV
Sbjct: 605 --AFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLV 664

Query: 218 MKDLGTK--RAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWI 277
           MKDLGTK  RAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWI
Sbjct: 665 MKDLGTKSIRAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWI 724

Query: 278 EGLRTIDGFIVGYGKGKVTRFPSD-------VPADMVVNAIIMAILTHKLQPSGRTIIYH 337
           EGLRTIDGFIVGYGKGKVTRFPSD       VPADMVVNAIIMAILTHKLQPSGRTIIYH
Sbjct: 725 EGLRTIDGFIVGYGKGKVTRFPSDVSSILDLVPADMVVNAIIMAILTHKLQPSGRTIIYH 784

Query: 338 IGSSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFL 397
           IGSSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFL
Sbjct: 785 IGSSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFL 844

Query: 398 KGFEFVNKAFCYSFQDKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDN 457
           KGFEFVNKAFCYSFQDKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDN
Sbjct: 845 KGFEFVNKAFCYSFQDKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDN 904

Query: 458 GINPNTSLLDPKDINWEDYFLNIHVPGLVKYTIK 483
           GINPNTSLLDPKDINWEDYFLNIHVPGLVKY +K
Sbjct: 905 GINPNTSLLDPKDINWEDYFLNIHVPGLVKYVMK 917

BLAST of CmoCh20G001920 vs. ExPASy TrEMBL
Match: A0A6J1FYH0 (Fatty acyl-CoA reductase OS=Cucurbita moschata OX=3662 GN=LOC111448658 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 2.7e-132
Identity = 283/529 (53.50%), Postives = 343/529 (64.84%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           +MVEK+LR HPN+KKLY+LLR+VDQITA KHF DEVVEKELF+VL EKWGGDLKTLVSEK
Sbjct: 21  IMVEKILRVHPNVKKLYILLRSVDQITAFKHFYDEVVEKELFRVLKEKWGGDLKTLVSEK 80

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I LV GDIS PNMGL DS+LLEEMKN+VEIIINFAA+TNFDER           YDVAFG
Sbjct: 81  IYLVPGDISYPNMGLNDSDLLEEMKNQVEIIINFAATTNFDER-----------YDVAFG 140

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITH---------------KLIETDVLSAFVSNQRE 182
           TN LGAKHV+NFAK CSN EILVH+ T                KL+E+   ++ +  + E
Sbjct: 141 TNTLGAKHVVNFAKGCSNLEILVHVSTAYVSNQREGVILETPCKLVESVDGTSKLDIESE 200

Query: 183 GVILE---TPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTK 242
             I+E      + +  ++ T++     +       +    N     K M  +++ D    
Sbjct: 201 RKIIEDSLRELRNNRDINETTRSLAMKDLGTKRSKMYGYPNTYTFTKAMGEMLVNDRTDN 260

Query: 243 RAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGF 302
              +   P+    T    +      I+ L  I                G+I G       
Sbjct: 261 LPLIIMRPSIVTSTY---KEPFPGWIEGLRTI---------------DGFIVGYAKGKVT 320

Query: 303 IVGYGKGKVTRFPSDVPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFS 362
               G G +      +PADMVVNAIIMAI  HKL+PS  TIIYH+ SSMRNS+      +
Sbjct: 321 HFPSGAGSILDL---IPADMVVNAIIMAISAHKLRPSKHTIIYHVSSSMRNSISNINSLN 380

Query: 363 YIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQ 422
           YI +YFT+KPW+N+DGK IK+KK+T FN++ASF RYMT+ Y VFLKGFEFVNKAF YSFQ
Sbjct: 381 YILKYFTEKPWINKDGKAIKIKKLTLFNDMASFRRYMTLRYLVFLKGFEFVNKAFFYSFQ 440

Query: 423 DKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDIN 482
           DKYNDLQRKFNWVMRQVELY+SF+FFKA+FDDTNL+KL+IVARDN INPNT LLDPKDIN
Sbjct: 441 DKYNDLQRKFNWVMRQVELYDSFLFFKARFDDTNLEKLQIVARDNNINPNTLLLDPKDIN 500

Query: 483 WEDYFLNIHVPGLVKYTIKFGKKLLEHLRSSLIGAATKKFDDELLAVVN 513
           WEDYFLNIHVPGLVKY +   +K L  + S   G   K    E + +V+
Sbjct: 501 WEDYFLNIHVPGLVKYVV---EKELFKVLSEKWGGDLKTLVSEKICLVS 514


HSP 2 Score: 740.7 bits (1911), Expect = 4.2e-210
Identity = 371/488 (76.02%), Postives = 421/488 (86.27%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           +MVEK+LR HPN+KKLYLLLRNVDQITASK F DEVVEKELF+VL EKWGGDLK LVSEK
Sbjct: 21  IMVEKILRAHPNVKKLYLLLRNVDQITASKRFYDEVVEKELFRVLKEKWGGDLKPLVSEK 80

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I LV GDISSPNMGLKDSNLLEEMKN+VEII+NFAA+TNFDER           YD+AF 
Sbjct: 81  IYLVPGDISSPNMGLKDSNLLEEMKNQVEIIVNFAATTNFDER-----------YDIAFS 140

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVD 182
           TN LGA+HVLNFA+QCS+ +ILVH+ T          A+VSNQREGVILETPCKL ESVD
Sbjct: 141 TNTLGARHVLNFAEQCSSLKILVHVST----------AYVSNQREGVILETPCKLVESVD 200

Query: 183 GTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMG 242
           GTSKL+IE+ERKIIE+SLRELRNNRDI++I RSL MKDLGTKRAKMYGYPNTY FTKAMG
Sbjct: 201 GTSKLDIETERKIIEDSLRELRNNRDIDEITRSLTMKDLGTKRAKMYGYPNTYVFTKAMG 260

Query: 243 EMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD--- 302
           EML++D+ DNL LII+RPTIVTSTYKEPFPGWIEGLRTIDG I GYGKGK+  FPS+   
Sbjct: 261 EMLISDKSDNLRLIIVRPTIVTSTYKEPFPGWIEGLRTIDGLIDGYGKGKLAFFPSNASS 320

Query: 303 ----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPW 362
               +PADMVVN IIMAI+ HKLQP G T+IYHIGSSMRN+M    L SYI QYFT+KPW
Sbjct: 321 ILDVIPADMVVNGIIMAIVAHKLQPFGHTLIYHIGSSMRNAMSNIDLHSYIFQYFTEKPW 380

Query: 363 MNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQDKYNDLQRKFN 422
           +++DGK +KVK +T FN++ASFHR+MTIHY +FLKGFEFVNKAFCY+FQDKYNDL+RKFN
Sbjct: 381 IDKDGKALKVKNVTLFNDMASFHRHMTIHYLIFLKGFEFVNKAFCYAFQDKYNDLRRKFN 440

Query: 423 WVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVP 482
           WVMRQVELY+SF+FFKAKFDDTNL++LRI ARDN INPNT LLDPKDI+WEDYFLNIH+P
Sbjct: 441 WVMRQVELYDSFLFFKAKFDDTNLERLRITARDNNINPNTLLLDPKDISWEDYFLNIHIP 487

BLAST of CmoCh20G001920 vs. ExPASy TrEMBL
Match: A0A6J1FTJ1 (Fatty acyl-CoA reductase OS=Cucurbita moschata OX=3662 GN=LOC111448659 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 2.3e-208
Identity = 369/488 (75.61%), Postives = 419/488 (85.86%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           +MVEK+LR H N+KKLYLLLRNVDQITASKHF DEVVEKELF+VL EKWGGDLKTL+SEK
Sbjct: 21  IMVEKILRAHHNVKKLYLLLRNVDQITASKHFYDEVVEKELFRVLKEKWGGDLKTLISEK 80

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I LV GDISSPNMGLKDSNLLEEMKN VEII+NFAA+TNFDER           YDVAF 
Sbjct: 81  IYLVPGDISSPNMGLKDSNLLEEMKNEVEIIVNFAATTNFDER-----------YDVAFS 140

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVD 182
           TN LGA+HVLNFA+QCSN +ILVH+ T          A+VSNQREGVILETPCKL ESVD
Sbjct: 141 TNTLGARHVLNFAEQCSNLKILVHVST----------AYVSNQREGVILETPCKLVESVD 200

Query: 183 GTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMG 242
           GTSKL+ E+ERKIIE+SLRELRNN+DI++I RSL MKDLGTKRAKMYGYPNTY FTKAMG
Sbjct: 201 GTSKLDFETERKIIEDSLRELRNNKDIDEITRSLTMKDLGTKRAKMYGYPNTYTFTKAMG 260

Query: 243 EMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD--- 302
           EML+ND+ DNL LII+RPTIVTSTYKEPFPGWIEGLRTIDG I GYGKGK+  FPS+   
Sbjct: 261 EMLINDKSDNLRLIIVRPTIVTSTYKEPFPGWIEGLRTIDGLIDGYGKGKLAFFPSNASS 320

Query: 303 ----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPW 362
               +PADMVVN IIMAIL HKLQP G T+IYHIGSSMRN+M    L SYI QYFT+KPW
Sbjct: 321 ILDVIPADMVVNGIIMAILAHKLQPFGHTLIYHIGSSMRNAMSNIDLCSYILQYFTEKPW 380

Query: 363 MNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQDKYNDLQRKFN 422
           +++DGK IK+KK+T FN++ASFHR+MTI Y +FLKGFEFVN+AFCY+FQDK NDL+ KF+
Sbjct: 381 IDKDGKTIKIKKLTLFNDMASFHRHMTIRYLIFLKGFEFVNRAFCYAFQDKCNDLRGKFD 440

Query: 423 WVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVP 482
           WVMRQVELY+SF+FFKA+FDDTNL+KLRI ARDN INPNTSLLDP+DINWEDYFLNIH+P
Sbjct: 441 WVMRQVELYDSFLFFKARFDDTNLEKLRIAARDNNINPNTSLLDPEDINWEDYFLNIHIP 487

BLAST of CmoCh20G001920 vs. ExPASy TrEMBL
Match: A0A6J1J966 (Fatty acyl-CoA reductase OS=Cucurbita maxima OX=3661 GN=LOC111484588 PE=3 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 7.4e-207
Identity = 371/488 (76.02%), Postives = 413/488 (84.63%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           +MVEK+LR HPN+KKLYLLLR+VDQITASKHF +EVVEKELF+VL EKWGGDLKTL+SEK
Sbjct: 21  LMVEKILRVHPNVKKLYLLLRSVDQITASKHFYNEVVEKELFRVLKEKWGGDLKTLISEK 80

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I LV GDIS PNMGL DSNLLEEMK++VEIIINFAA+TNFDER           YDVAFG
Sbjct: 81  IYLVPGDISFPNMGLNDSNLLEEMKSQVEIIINFAATTNFDER-----------YDVAFG 140

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVD 182
           TN LGA+HV+NFAK CSN EILVH+ T          A+VSNQREGVILE PCKL ESVD
Sbjct: 141 TNTLGAEHVVNFAKGCSNLEILVHVST----------AYVSNQREGVILEIPCKLVESVD 200

Query: 183 GTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMG 242
           GTSKL+IESERKIIE+SLRELRNNRDIN+  RS+ MKDLGTKR+KMYGYPNTY FTKAMG
Sbjct: 201 GTSKLDIESERKIIEDSLRELRNNRDINETTRSVAMKDLGTKRSKMYGYPNTYTFTKAMG 260

Query: 243 EMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD--- 302
           EML+ND+ DNLPLIIMRP IVTSTYKEPFPGWIEGLRTID FIVGY KGKVT FPS    
Sbjct: 261 EMLVNDKTDNLPLIIMRPAIVTSTYKEPFPGWIEGLRTIDSFIVGYAKGKVTHFPSAASS 320

Query: 303 ----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPW 362
               +PADMVVNAIIMAIL HKL+PS   IIYH+ SSM+NS+      +YI +YFT+KPW
Sbjct: 321 ILDLIPADMVVNAIIMAILAHKLRPSRHKIIYHVSSSMKNSISNINFQNYILRYFTEKPW 380

Query: 363 MNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQDKYNDLQRKFN 422
           +N+DGK IK+KK+T FN++ SFHRYMTI Y VFLKGFE VNK F YSFQDKYNDLQRKFN
Sbjct: 381 INKDGKAIKIKKLTLFNDMTSFHRYMTIRYMVFLKGFELVNKTFFYSFQDKYNDLQRKFN 440

Query: 423 WVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVP 482
           WVMRQVELY+SF+FFKA+FDDTNL+KL++VARDN IN NT LLDPKDINWEDYFLNIHVP
Sbjct: 441 WVMRQVELYDSFLFFKARFDDTNLEKLQMVARDNNINQNTLLLDPKDINWEDYFLNIHVP 487

BLAST of CmoCh20G001920 vs. ExPASy TrEMBL
Match: A0A6J1DGK9 (Fatty acyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC111020672 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 2.0e-175
Identity = 312/490 (63.67%), Postives = 386/490 (78.78%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           +MVEK+LR  PN+KKLYLLLR+VD+ITA+K F DEVVEK+LF++L EKW G+L T +SE+
Sbjct: 21  IMVEKILRVQPNVKKLYLLLRSVDEITATKRFYDEVVEKDLFRLLKEKWSGNLDTFISER 80

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I LV GD++ PNMGLKDSNLL+EMKN+VEIIIN AA+TNFDER           YDVAFG
Sbjct: 81  ISLVHGDVACPNMGLKDSNLLKEMKNQVEIIINLAATTNFDER-----------YDVAFG 140

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQRE-GVILETPCKLDESV 182
           TN LGAKHVL+FAK+CSN ++L+H+ T          A+VS +R+ G+ILETPCKLDES 
Sbjct: 141 TNTLGAKHVLSFAKKCSNLKVLIHIST----------AYVSGERKGGLILETPCKLDESS 200

Query: 183 DGTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAM 242
           +   KL+IE E+K+IEE+L E RN +   + + +L MKDLG KRA +YG PNTY FTKAM
Sbjct: 201 NKAIKLDIEIEQKVIEETLNEFRNKKSTEETI-TLAMKDLGLKRAMIYGCPNTYTFTKAM 260

Query: 243 GEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD-- 302
           GEM+++D  D +PLII+RPTIVTSTYKEPFPGWIEGLRTID FI GY KGKVT FP D  
Sbjct: 261 GEMMVSDEKDGIPLIIIRPTIVTSTYKEPFPGWIEGLRTIDSFIAGYAKGKVTCFPCDDK 320

Query: 303 -----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKP 362
                +PADMV+NAIIMAI+TH  QPS +T+IYH+GSS RNS+R     +Y+ +YFTKKP
Sbjct: 321 SILDLIPADMVINAIIMAIVTHMFQPSNQTLIYHVGSSTRNSIRHMDFHNYLLRYFTKKP 380

Query: 363 WMNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQDKYNDLQRKF 422
           W+N+DG +I+ KKIT FN++ SFHRYMT+ Y +FLKG EFVNKA CYSFQD Y DLQRKF
Sbjct: 381 WINKDGSIIQSKKITLFNDMESFHRYMTLRYLIFLKGLEFVNKALCYSFQDTYIDLQRKF 440

Query: 423 NWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINP-NTSLLDPKDINWEDYFLNIH 482
           NWVMRQ+ELY  ++FFK+KFDDTN +KLRI  ++   N  NT  +DPKDI+WEDYF+NIH
Sbjct: 441 NWVMRQIELYRPYLFFKSKFDDTNFEKLRIATQECDRNAINTLFMDPKDISWEDYFVNIH 488

BLAST of CmoCh20G001920 vs. NCBI nr
Match: KAG6570487.1 (Fatty acyl-CoA reductase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 830.9 bits (2145), Expect = 6.4e-237
Identity = 421/452 (93.14%), Postives = 426/452 (94.25%), Query Frame = 0

Query: 38   VVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFA 97
            VVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFA
Sbjct: 1322 VVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFA 1381

Query: 98   ASTNFDERYRLSRVVLIRKYDVAFGTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDV 157
            ASTNFDER           YDVAFGTNVLGAKHVLNFAKQCSNFEILVH      + TDV
Sbjct: 1382 ASTNFDER-----------YDVAFGTNVLGAKHVLNFAKQCSNFEILVH------VSTDV 1441

Query: 158  LSAFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLV 217
            LSAFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDIN+IMRSLV
Sbjct: 1442 LSAFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINEIMRSLV 1501

Query: 218  MKDLGTKRAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEG 277
            MKDLGTKRAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTI+TSTYKEPFPGWIEG
Sbjct: 1502 MKDLGTKRAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIITSTYKEPFPGWIEG 1561

Query: 278  LRTIDGFIVGYGKGKVTRFPSD-------VPADMVVNAIIMAILTHKLQPSGRTIIYHIG 337
            LRTIDGFIVGYGKGKVTRFPSD       VPADMVVNAIIMAILTHKLQPSGRTIIYHIG
Sbjct: 1562 LRTIDGFIVGYGKGKVTRFPSDVSSILDLVPADMVVNAIIMAILTHKLQPSGRTIIYHIG 1621

Query: 338  SSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFLKG 397
            SSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFLKG
Sbjct: 1622 SSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFLKG 1681

Query: 398  FEFVNKAFCYSFQDKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGI 457
            FEFVNKAFCYSFQDKYNDL+RKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGI
Sbjct: 1682 FEFVNKAFCYSFQDKYNDLRRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGI 1741

Query: 458  NPNTSLLDPKDINWEDYFLNIHVPGLVKYTIK 483
            NPNTSLLDPKDINWEDYFLNIHVPGLVKY +K
Sbjct: 1742 NPNTSLLDPKDINWEDYFLNIHVPGLVKYVMK 1756

BLAST of CmoCh20G001920 vs. NCBI nr
Match: KAG6570487.1 (Fatty acyl-CoA reductase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 481.1 bits (1237), Expect = 1.2e-131
Identity = 271/498 (54.42%), Postives = 331/498 (66.47%), Query Frame = 0

Query: 4   MVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEKI 63
           MVEK+LR H N+KKLYLLLRNVDQIT SKHF DEVVEKELF+VL EKWGGDLKTL+SEKI
Sbjct: 1   MVEKILRAHHNVKKLYLLLRNVDQITVSKHFYDEVVEKELFRVLKEKWGGDLKTLISEKI 60

Query: 64  CLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFGT 123
            LV GDISSPNMGLKDSNLLEEMKN VEII+NFAA+TNFDER           YDVAF T
Sbjct: 61  YLVPGDISSPNMGLKDSNLLEEMKNEVEIIVNFAATTNFDER-----------YDVAFST 120

Query: 124 NVLGAKHVLNFAKQCSNFEILVHLIT--------HKLIETDVLSAFVSNQREGVILETPC 183
           N LGA+HVLNFA+QCSN +ILVH+ T          ++ET        +    + +ET  
Sbjct: 121 NTLGARHVLNFAEQCSNLKILVHVSTAYVSNQREGVILETPCTLVESVDGTSKLDIETER 180

Query: 184 KLDESVDGTSKLNIESERKIIEESLRELRNNR----------DINKIMRSLVMKDLGTKR 243
           K+ E      + N + +      ++++L   R             K M  +++ D     
Sbjct: 181 KIIEDSLRELRNNRDIDEITRSLTMKDLGTKRAKMYGYPNTYTFTKAMGEMLINDKSDNL 240

Query: 244 AKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFI 303
             +   P     T    +      I+ L  I                G I+G        
Sbjct: 241 RLIIVRPTIVTSTY---KEPFPGWIEGLRTI---------------DGLIDGYGKGK--- 300

Query: 304 VGYGKGKVTRFPSDVPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSY 363
           + +     +     +PADMVVN IIMAIL HKLQP G T+IYHIGSSMRN+M    L SY
Sbjct: 301 LAFFPSNASSILDVIPADMVVNGIIMAILAHKLQPFGHTLIYHIGSSMRNAMSNIDLCSY 360

Query: 364 IHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQD 423
           I QYFT+KPW+++DGK IK+KK+T FN++ASFHR+MTI Y +FLKGFEFVN+AFCY+FQD
Sbjct: 361 ILQYFTEKPWIDKDGKTIKIKKLTLFNDMASFHRHMTIRYLIFLKGFEFVNRAFCYAFQD 420

Query: 424 KYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINW 483
           K NDL+ KF+WVMRQVELY+SF+FFKA+FDDTNL+KLRI ARDN INPNTSLLDP+DINW
Sbjct: 421 KCNDLRGKFDWVMRQVELYDSFLFFKARFDDTNLEKLRIAARDNNINPNTSLLDPEDINW 466


HSP 2 Score: 819.3 bits (2115), Expect = 1.9e-233
Identity = 420/454 (92.51%), Postives = 422/454 (92.95%), Query Frame = 0

Query: 38  VVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFA 97
           VVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFA
Sbjct: 485 VVEKELFKVLSEKWGGDLKTLVSEKICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFA 544

Query: 98  ASTNFDERYRLSRVVLIRKYDVAFGTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDV 157
           ASTNFDER           YDVAFGTNVLGAKHVLNFAKQCSNFEILVH+ T        
Sbjct: 545 ASTNFDER-----------YDVAFGTNVLGAKHVLNFAKQCSNFEILVHVST-------- 604

Query: 158 LSAFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLV 217
             AFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLV
Sbjct: 605 --AFVSNQREGVILETPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLV 664

Query: 218 MKDLGTK--RAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWI 277
           MKDLGTK  RAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWI
Sbjct: 665 MKDLGTKSIRAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWI 724

Query: 278 EGLRTIDGFIVGYGKGKVTRFPSD-------VPADMVVNAIIMAILTHKLQPSGRTIIYH 337
           EGLRTIDGFIVGYGKGKVTRFPSD       VPADMVVNAIIMAILTHKLQPSGRTIIYH
Sbjct: 725 EGLRTIDGFIVGYGKGKVTRFPSDVSSILDLVPADMVVNAIIMAILTHKLQPSGRTIIYH 784

Query: 338 IGSSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFL 397
           IGSSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFL
Sbjct: 785 IGSSMRNSMRIDTLFSYIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVFL 844

Query: 398 KGFEFVNKAFCYSFQDKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDN 457
           KGFEFVNKAFCYSFQDKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDN
Sbjct: 845 KGFEFVNKAFCYSFQDKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDN 904

Query: 458 GINPNTSLLDPKDINWEDYFLNIHVPGLVKYTIK 483
           GINPNTSLLDPKDINWEDYFLNIHVPGLVKY +K
Sbjct: 905 GINPNTSLLDPKDINWEDYFLNIHVPGLVKYVMK 917

BLAST of CmoCh20G001920 vs. NCBI nr
Match: XP_022944110.1 (fatty acyl-CoA reductase 3-like [Cucurbita moschata])

HSP 1 Score: 482.3 bits (1240), Expect = 5.6e-132
Identity = 283/529 (53.50%), Postives = 343/529 (64.84%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           +MVEK+LR HPN+KKLY+LLR+VDQITA KHF DEVVEKELF+VL EKWGGDLKTLVSEK
Sbjct: 21  IMVEKILRVHPNVKKLYILLRSVDQITAFKHFYDEVVEKELFRVLKEKWGGDLKTLVSEK 80

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I LV GDIS PNMGL DS+LLEEMKN+VEIIINFAA+TNFDER           YDVAFG
Sbjct: 81  IYLVPGDISYPNMGLNDSDLLEEMKNQVEIIINFAATTNFDER-----------YDVAFG 140

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITH---------------KLIETDVLSAFVSNQRE 182
           TN LGAKHV+NFAK CSN EILVH+ T                KL+E+   ++ +  + E
Sbjct: 141 TNTLGAKHVVNFAKGCSNLEILVHVSTAYVSNQREGVILETPCKLVESVDGTSKLDIESE 200

Query: 183 GVILE---TPCKLDESVDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTK 242
             I+E      + +  ++ T++     +       +    N     K M  +++ D    
Sbjct: 201 RKIIEDSLRELRNNRDINETTRSLAMKDLGTKRSKMYGYPNTYTFTKAMGEMLVNDRTDN 260

Query: 243 RAKMYGYPNTYAFTKAMGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGF 302
              +   P+    T    +      I+ L  I                G+I G       
Sbjct: 261 LPLIIMRPSIVTSTY---KEPFPGWIEGLRTI---------------DGFIVGYAKGKVT 320

Query: 303 IVGYGKGKVTRFPSDVPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFS 362
               G G +      +PADMVVNAIIMAI  HKL+PS  TIIYH+ SSMRNS+      +
Sbjct: 321 HFPSGAGSILDL---IPADMVVNAIIMAISAHKLRPSKHTIIYHVSSSMRNSISNINSLN 380

Query: 363 YIHQYFTKKPWMNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQ 422
           YI +YFT+KPW+N+DGK IK+KK+T FN++ASF RYMT+ Y VFLKGFEFVNKAF YSFQ
Sbjct: 381 YILKYFTEKPWINKDGKAIKIKKLTLFNDMASFRRYMTLRYLVFLKGFEFVNKAFFYSFQ 440

Query: 423 DKYNDLQRKFNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDIN 482
           DKYNDLQRKFNWVMRQVELY+SF+FFKA+FDDTNL+KL+IVARDN INPNT LLDPKDIN
Sbjct: 441 DKYNDLQRKFNWVMRQVELYDSFLFFKARFDDTNLEKLQIVARDNNINPNTLLLDPKDIN 500

Query: 483 WEDYFLNIHVPGLVKYTIKFGKKLLEHLRSSLIGAATKKFDDELLAVVN 513
           WEDYFLNIHVPGLVKY +   +K L  + S   G   K    E + +V+
Sbjct: 501 WEDYFLNIHVPGLVKYVV---EKELFKVLSEKWGGDLKTLVSEKICLVS 514


HSP 2 Score: 747.3 bits (1928), Expect = 9.3e-212
Identity = 383/488 (78.48%), Postives = 417/488 (85.45%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           +MVEK+LR HPN+KKLYLLLR+VD ITASKHF +EVVEKELF+VL EKWGGDLK L+SEK
Sbjct: 21  LMVEKILRVHPNVKKLYLLLRSVDHITASKHFYNEVVEKELFRVLKEKWGGDLKPLISEK 80

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I LV GDISSPNMGL DS LLEEMKN+VEIIINFAA+TNFDER           YDVAFG
Sbjct: 81  IYLVPGDISSPNMGLNDSGLLEEMKNQVEIIINFAATTNFDER-----------YDVAFG 140

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVD 182
           TN LGAKHVLNFAK CSN EILVH+ T          A+VSNQREGVILETPCKL ESVD
Sbjct: 141 TNTLGAKHVLNFAKGCSNLEILVHVST----------AYVSNQREGVILETPCKLVESVD 200

Query: 183 GTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMG 242
           GTSKL+IESERKIIE+SLRELRNNRDIN+  RSL MKDLGTKR+KMYGYPNTY FTKAMG
Sbjct: 201 GTSKLDIESERKIIEDSLRELRNNRDINETTRSLAMKDLGTKRSKMYGYPNTYTFTKAMG 260

Query: 243 EMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD--- 302
           EML+NDR DNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGY KGKVT FPS    
Sbjct: 261 EMLVNDRTDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYAKGKVTHFPSGAGS 320

Query: 303 ----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPW 362
               +PADMVVNAIIMAI  HKLQPS  TIIYH+ SSM+NS+      +YI QYFT+KPW
Sbjct: 321 IFDLIPADMVVNAIIMAISAHKLQPSRHTIIYHVSSSMKNSISNINFLNYILQYFTEKPW 380

Query: 363 MNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQDKYNDLQRKFN 422
           +N+DGK IK+KK+T FN++ASFHRYMTI Y VFLKGFEFVNKAF YSFQDKYNDLQRKFN
Sbjct: 381 INKDGKAIKIKKLTLFNDMASFHRYMTIRYLVFLKGFEFVNKAFFYSFQDKYNDLQRKFN 440

Query: 423 WVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVP 482
            VMRQVELY+SF+FFKA+FDDTNL+KL+IVARDN INPNTSLLDPK INWEDYFLNIHVP
Sbjct: 441 RVMRQVELYDSFLFFKARFDDTNLEKLQIVARDNNINPNTSLLDPKAINWEDYFLNIHVP 487

BLAST of CmoCh20G001920 vs. NCBI nr
Match: XP_022985825.1 (fatty acyl-CoA reductase 3-like [Cucurbita maxima])

HSP 1 Score: 740.7 bits (1911), Expect = 8.7e-210
Identity = 371/488 (76.02%), Postives = 421/488 (86.27%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           +MVEK+LR HPN+KKLYLLLRNVDQITASK F DEVVEKELF+VL EKWGGDLK LVSEK
Sbjct: 21  IMVEKILRAHPNVKKLYLLLRNVDQITASKRFYDEVVEKELFRVLKEKWGGDLKPLVSEK 80

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I LV GDISSPNMGLKDSNLLEEMKN+VEII+NFAA+TNFDER           YD+AF 
Sbjct: 81  IYLVPGDISSPNMGLKDSNLLEEMKNQVEIIVNFAATTNFDER-----------YDIAFS 140

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVD 182
           TN LGA+HVLNFA+QCS+ +ILVH+ T          A+VSNQREGVILETPCKL ESVD
Sbjct: 141 TNTLGARHVLNFAEQCSSLKILVHVST----------AYVSNQREGVILETPCKLVESVD 200

Query: 183 GTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMG 242
           GTSKL+IE+ERKIIE+SLRELRNNRDI++I RSL MKDLGTKRAKMYGYPNTY FTKAMG
Sbjct: 201 GTSKLDIETERKIIEDSLRELRNNRDIDEITRSLTMKDLGTKRAKMYGYPNTYVFTKAMG 260

Query: 243 EMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD--- 302
           EML++D+ DNL LII+RPTIVTSTYKEPFPGWIEGLRTIDG I GYGKGK+  FPS+   
Sbjct: 261 EMLISDKSDNLRLIIVRPTIVTSTYKEPFPGWIEGLRTIDGLIDGYGKGKLAFFPSNASS 320

Query: 303 ----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPW 362
               +PADMVVN IIMAI+ HKLQP G T+IYHIGSSMRN+M    L SYI QYFT+KPW
Sbjct: 321 ILDVIPADMVVNGIIMAIVAHKLQPFGHTLIYHIGSSMRNAMSNIDLHSYIFQYFTEKPW 380

Query: 363 MNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQDKYNDLQRKFN 422
           +++DGK +KVK +T FN++ASFHR+MTIHY +FLKGFEFVNKAFCY+FQDKYNDL+RKFN
Sbjct: 381 IDKDGKALKVKNVTLFNDMASFHRHMTIHYLIFLKGFEFVNKAFCYAFQDKYNDLRRKFN 440

Query: 423 WVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVP 482
           WVMRQVELY+SF+FFKAKFDDTNL++LRI ARDN INPNT LLDPKDI+WEDYFLNIH+P
Sbjct: 441 WVMRQVELYDSFLFFKAKFDDTNLERLRITARDNNINPNTLLLDPKDISWEDYFLNIHIP 487

BLAST of CmoCh20G001920 vs. NCBI nr
Match: XP_022944111.1 (fatty acyl-CoA reductase 3-like [Cucurbita moschata])

HSP 1 Score: 734.9 bits (1896), Expect = 4.8e-208
Identity = 369/488 (75.61%), Postives = 419/488 (85.86%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           +MVEK+LR H N+KKLYLLLRNVDQITASKHF DEVVEKELF+VL EKWGGDLKTL+SEK
Sbjct: 21  IMVEKILRAHHNVKKLYLLLRNVDQITASKHFYDEVVEKELFRVLKEKWGGDLKTLISEK 80

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I LV GDISSPNMGLKDSNLLEEMKN VEII+NFAA+TNFDER           YDVAF 
Sbjct: 81  IYLVPGDISSPNMGLKDSNLLEEMKNEVEIIVNFAATTNFDER-----------YDVAFS 140

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVD 182
           TN LGA+HVLNFA+QCSN +ILVH+ T          A+VSNQREGVILETPCKL ESVD
Sbjct: 141 TNTLGARHVLNFAEQCSNLKILVHVST----------AYVSNQREGVILETPCKLVESVD 200

Query: 183 GTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMG 242
           GTSKL+ E+ERKIIE+SLRELRNN+DI++I RSL MKDLGTKRAKMYGYPNTY FTKAMG
Sbjct: 201 GTSKLDFETERKIIEDSLRELRNNKDIDEITRSLTMKDLGTKRAKMYGYPNTYTFTKAMG 260

Query: 243 EMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD--- 302
           EML+ND+ DNL LII+RPTIVTSTYKEPFPGWIEGLRTIDG I GYGKGK+  FPS+   
Sbjct: 261 EMLINDKSDNLRLIIVRPTIVTSTYKEPFPGWIEGLRTIDGLIDGYGKGKLAFFPSNASS 320

Query: 303 ----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPW 362
               +PADMVVN IIMAIL HKLQP G T+IYHIGSSMRN+M    L SYI QYFT+KPW
Sbjct: 321 ILDVIPADMVVNGIIMAILAHKLQPFGHTLIYHIGSSMRNAMSNIDLCSYILQYFTEKPW 380

Query: 363 MNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQDKYNDLQRKFN 422
           +++DGK IK+KK+T FN++ASFHR+MTI Y +FLKGFEFVN+AFCY+FQDK NDL+ KF+
Sbjct: 381 IDKDGKTIKIKKLTLFNDMASFHRHMTIRYLIFLKGFEFVNRAFCYAFQDKCNDLRGKFD 440

Query: 423 WVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVP 482
           WVMRQVELY+SF+FFKA+FDDTNL+KLRI ARDN INPNTSLLDP+DINWEDYFLNIH+P
Sbjct: 441 WVMRQVELYDSFLFFKARFDDTNLEKLRIAARDNNINPNTSLLDPEDINWEDYFLNIHIP 487

BLAST of CmoCh20G001920 vs. TAIR 10
Match: AT4G33790.1 (Jojoba acyl CoA reductase-related male sterility protein )

HSP 1 Score: 431.8 bits (1109), Expect = 8.1e-121
Identity = 226/485 (46.60%), Postives = 322/485 (66.39%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGDLKTLVSEK 62
           + VEK+LR  PN+KKLYLLLR     +A++ F DE+++K+LFKVL EK+G +L  L SEK
Sbjct: 31  IFVEKILRVAPNVKKLYLLLRASKGKSATQRFNDEILKKDLFKVLKEKYGPNLNQLTSEK 90

Query: 63  ICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAFG 122
           I +V GDI   ++GL+D +L  EM ++V+ I+N AA+T FDER           YDVA G
Sbjct: 91  ITIVDGDICLEDLGLQDFDLAHEMIHQVDAIVNLAATTKFDER-----------YDVALG 150

Query: 123 TNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESVD 182
            N LGA +VLNFAK+C+  +ILVH+ T          A+V  ++ G+I+ETP ++ E+++
Sbjct: 151 INTLGALNVLNFAKRCAKVKILVHVST----------AYVCGEKSGLIMETPYRMGETLN 210

Query: 183 GTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAMG 242
           GT+ L+I  E+K+++E L +LR      + +    MKDLG +RAKMYG+PNTY FTKAMG
Sbjct: 211 GTTGLDINYEKKLVQEKLDQLRVIGAAPETITE-TMKDLGLRRAKMYGWPNTYVFTKAMG 270

Query: 243 EMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD--- 302
           EM++  + +NL L+++RP+I+TST+KEPFPGW EG+RTID   VGYGKGK+T F  D   
Sbjct: 271 EMMVGTKRENLSLVLLRPSIITSTFKEPFPGWTEGIRTIDSLAVGYGKGKLTCFLCDLDA 330

Query: 303 ----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKPW 362
               +PADMVVN+I++++           IIYH+GSS+RN M+        ++YF+ KPW
Sbjct: 331 VSDVMPADMVVNSILVSMAAQ--AGKQEEIIYHVGSSLRNPMKNSKFPELAYRYFSIKPW 390

Query: 363 MNEDGKVIKVKKITSFNNIASFHRYMTIHY-VFLKGFEFVNKAFCYSFQDKYNDLQRKFN 422
            N++GKV+KV  I   +++ SFHRYMTI Y + LKG E VN   C  F+ ++    +K N
Sbjct: 391 TNKEGKVVKVGAIEILSSMRSFHRYMTIRYLIALKGLELVNIILCKLFEKEFQYFNKKIN 450

Query: 423 WVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLLDPKDINWEDYFLNIHVP 480
           ++ R V+LY  ++FF   FDD+N +KLR +    G+       DPK ++W+DYFLN HV 
Sbjct: 451 FIFRLVDLYQPYLFFYGIFDDSNTEKLRKMVSKTGVENEMFYFDPKVLDWDDYFLNTHVI 491

BLAST of CmoCh20G001920 vs. TAIR 10
Match: AT5G22500.1 (fatty acid reductase 1 )

HSP 1 Score: 386.3 bits (991), Expect = 3.9e-107
Identity = 213/490 (43.47%), Postives = 305/490 (62.24%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGD-LKTLVSE 62
           V+VEK+LR  PN+KK+YLLLR  D+ +A +    EV+E +LFKVL    G D L  L+ E
Sbjct: 26  VLVEKILRLQPNVKKIYLLLRAPDEKSAMQRLRSEVMEIDLFKVLRNNLGEDNLNALMRE 85

Query: 63  KICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAF 122
           KI  V GDIS  N+GLKD++L++ M + ++IIIN AA+TNFDER           YD+  
Sbjct: 86  KIVPVPGDISIDNLGLKDTDLIQRMWSEIDIIINIAATTNFDER-----------YDIGL 145

Query: 123 GTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESV 182
           G N  GA +VLNFAK+C   ++L+H+ T          A++S ++ G++LE P K+ E++
Sbjct: 146 GINTFGALNVLNFAKKCVKGQLLLHVST----------AYISGEQPGLLLEKPFKMGETL 205

Query: 183 DGTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKAM 242
            G  +L+I  E  ++++ L+EL   +D +    S  MKD G  RAK++G+PNTY FTKAM
Sbjct: 206 SGDRELDINIEHDLMKQKLKEL---QDCSDEEISQTMKDFGMARAKLHGWPNTYVFTKAM 265

Query: 243 GEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD-- 302
           GEMLM    +NLPL+I+RPT++TST  EPFPGWIEGL+T+D  IV YGKG++  F +D  
Sbjct: 266 GEMLMGKYRENLPLVIIRPTMITSTIAEPFPGWIEGLKTLDSVIVAYGKGRLKCFLADSN 325

Query: 303 -----VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKKP 362
                +PADMVVNA++ A   H    +G   IYH+GSS +N +    L  +  +YF K+P
Sbjct: 326 SVFDLIPADMVVNAMVAAATAHS-GDTGIQAIYHVGSSCKNPVTFGQLHDFTARYFAKRP 385

Query: 363 WMNEDGKVIKVKKITSFNNIASFHRYMTIHYVF-LKGFEFVNKAFCYSFQDKYNDLQRKF 422
            +  +G  I V K T  + +A F  YMT+ Y   L+    +N  + +S  D Y+DL RK 
Sbjct: 386 LIGRNGSPIIVVKGTILSTMAQFSLYMTLRYKLPLQILRLINIVYPWSHGDNYSDLSRKI 445

Query: 423 NWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSL-LDPKDINWEDYFLNIH 482
              MR VELY  ++ FK  FDD N ++LR+  ++N    + S   DPK I+W++Y  N H
Sbjct: 446 KLAMRLVELYQPYLLFKGIFDDLNTERLRMKRKENIKELDGSFEFDPKSIDWDNYITNTH 490

BLAST of CmoCh20G001920 vs. TAIR 10
Match: AT3G44540.1 (fatty acid reductase 4 )

HSP 1 Score: 375.6 bits (963), Expect = 6.9e-104
Identity = 209/493 (42.39%), Postives = 303/493 (61.46%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGD-LKTLVSE 62
           V VEK+LR  P +KKL+LLLR  D  +A + F  EV+EK+LF+VL    G + LK  ++E
Sbjct: 26  VFVEKILRIQPKVKKLFLLLRAADNESAMQRFHSEVLEKDLFRVLKNALGDENLKAFITE 85

Query: 63  KICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAF 122
           K+  + GDIS  N+G+K S+LL+ M N ++II+N AA+TNFDER           YDV  
Sbjct: 86  KVVPIPGDISVDNLGVKGSDLLQHMWNEIDIIVNVAATTNFDER-----------YDVGL 145

Query: 123 GTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESV 182
             N  G  +VLNFAK+C   ++L+H+ T          A+V  ++ G++ E    + E++
Sbjct: 146 SVNTFGPLNVLNFAKKCVKGQLLLHVST----------AYVRGEKSGLLHEKTFHMGETL 205

Query: 183 DGTSKLNIESERKIIEESLREL-RNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKA 242
           +G  KL IE+E +++++ L+EL + N    +I +S  MKDLG  RAK++G+PNTY FTK+
Sbjct: 206 NGHRKLVIETEMELMKQKLKELQKQNCSEEEISQS--MKDLGMSRAKLHGWPNTYVFTKS 265

Query: 243 MGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSD- 302
           MGEML+ +  +NLP++I+RPT++TST+ EPFPGWIEGLRTID  IV YGKG++  F +D 
Sbjct: 266 MGEMLLGNYRENLPIVIIRPTMITSTFSEPFPGWIEGLRTIDSVIVAYGKGRLKCFLADP 325

Query: 303 ------VPADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKK 362
                 +P DMV NA++ A   H  +   +T +YH+GSS +N +  + +      YFTK 
Sbjct: 326 NSVLDLIPVDMVANAMVTAAAIHAGKLGSQT-VYHVGSSCKNPITFEQIHDLAASYFTKN 385

Query: 363 PWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVF-LKGFEFVNKAFCYSFQDKYNDLQRK 422
           P +  DG  I V K T  + +A F  YMT+ Y   L+    +   + +   +KY D+ RK
Sbjct: 386 PLVRRDGSSILVSKGTILSTMAQFSFYMTLRYKLPLQMLRLIYVIYPWWNGNKYKDIDRK 445

Query: 423 FNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLL---DPKDINWEDYFL 482
               MR V+LY  ++ FK  FDDTN +KLR+  ++  IN     L   DPK I+WEDY  
Sbjct: 446 IKLAMRLVDLYRPYVLFKGIFDDTNTEKLRLKRKE--INKEMYGLFEFDPKSIDWEDYMT 492

BLAST of CmoCh20G001920 vs. TAIR 10
Match: AT3G44550.1 (fatty acid reductase 5 )

HSP 1 Score: 369.4 bits (947), Expect = 4.9e-102
Identity = 212/493 (43.00%), Postives = 298/493 (60.45%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGD-LKTLVSE 62
           V VEK+LR  PN+KKLYLL+R  D   A+K    EV EKELFKVL +  G + L TL+ E
Sbjct: 26  VFVEKILRVQPNVKKLYLLVRASDNEAATKRLRTEVFEKELFKVLRQNLGDEKLNTLLYE 85

Query: 63  KICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAF 122
           K+  V GDI++  +G+ DS+L E M+  ++I++N AA+TNFDER           YDV  
Sbjct: 86  KVVSVPGDIATDQLGINDSHLRERMQKEIDIVVNVAATTNFDER-----------YDVGL 145

Query: 123 GTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDE-S 182
           G N  GA +VLNFAK+C   ++L+H+ T          A+V  ++ G+I E P  ++E  
Sbjct: 146 GINTFGALNVLNFAKKCVKVQLLLHVST----------AYVCGEKPGLIPEKPFIMEEIR 205

Query: 183 VDGTSKLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKA 242
            +   +L+I  ER+++++ L+EL N +D ++   +L MK+LG +RAK++G+PNTY FTK+
Sbjct: 206 NENGLQLDINLERELMKQRLKEL-NEQDCSEEDITLSMKELGMERAKLHGWPNTYVFTKS 265

Query: 243 MGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSDV 302
           MGEML+    +NLPL+I+RPT++TST  EPFPGWIEGLRT+D  I+ YGKG +  F  DV
Sbjct: 266 MGEMLLGKHKENLPLVIIRPTMITSTLSEPFPGWIEGLRTVDSVIIAYGKGVLKCFLVDV 325

Query: 303 -------PADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKK 362
                  P DMV NA+I A   H    SG  ++YH+GSS +N +    +     +YFTK 
Sbjct: 326 NSVCDMIPVDMVANAMITAAAKH-AGGSGVHMVYHVGSSHQNPVTFGEIHEIAVRYFTKN 385

Query: 363 PWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVF-LKGFEFVNKAFCYSFQDKYNDLQRK 422
           P  + +G +I V K+     +A F  YMT+ Y   L+  + V+  + +   DKY D  RK
Sbjct: 386 PLRSRNGSLITVSKVRFIPTMALFSLYMTLRYKLPLQLLKLVDIIYPWRNGDKYGDKNRK 445

Query: 423 FNWVMRQVELYNSFIFFKAKFDDTNLKKLRIVARDNGINPNTSLL---DPKDINWEDYFL 482
              VMR VELY  ++ FK  FDD N K L    ++  I     L+   DPK INW DY  
Sbjct: 446 IELVMRLVELYEPYVLFKGIFDDRNTKSLCANQKEEEIKNTEKLMFDFDPKGINWGDYLT 495

BLAST of CmoCh20G001920 vs. TAIR 10
Match: AT3G44560.1 (fatty acid reductase 8 )

HSP 1 Score: 351.7 bits (901), Expect = 1.1e-96
Identity = 202/493 (40.97%), Postives = 299/493 (60.65%), Query Frame = 0

Query: 3   VMVEKLLRTHPNLKKLYLLLRNVDQITASKHFCDEVVEKELFKVLSEKWGGD-LKTLVSE 62
           V VEK+LR  PN+ KLYL++R  D   A+K    E  EK+LFKVL +  G + L TL+SE
Sbjct: 26  VFVEKILRVQPNVNKLYLVVRASDNEAATKRLRTEAFEKDLFKVLRDNLGDEKLNTLLSE 85

Query: 63  KICLVSGDISSPNMGLKDSNLLEEMKNRVEIIINFAASTNFDERYRLSRVVLIRKYDVAF 122
           K+  V+GDI+  ++G+KDSNL E M+  ++I++N AA+TNFDER           YD+  
Sbjct: 86  KVVPVAGDIAMDHLGMKDSNLRERMQKEIDIVVNVAATTNFDER-----------YDIGL 145

Query: 123 GTNVLGAKHVLNFAKQCSNFEILVHLITHKLIETDVLSAFVSNQREGVILETPCKLDESV 182
           G N  GA +VLNFAK+C   ++L+H+ T          A+V  ++ G++ E P  ++E  
Sbjct: 146 GINTFGALNVLNFAKKCVKAQLLLHVST----------AYVCGEKPGLLPEKPFVMEEIC 205

Query: 183 DGTS-KLNIESERKIIEESLRELRNNRDINKIMRSLVMKDLGTKRAKMYGYPNTYAFTKA 242
           +    +L+I  ER+++++ L+EL N +  ++   +  MK+LG +RAK++G+PNTY FTK+
Sbjct: 206 NENGLQLDINLERELMKQRLKEL-NEQGCSEEGTTFYMKELGMERAKLHGWPNTYVFTKS 265

Query: 243 MGEMLMNDRIDNLPLIIMRPTIVTSTYKEPFPGWIEGLRTIDGFIVGYGKGKVTRFPSDV 302
           MGEML+ +  +NLPL+I+RPT++TST  EPFPGWIEGLRT+D  I+ YGKG +  F  DV
Sbjct: 266 MGEMLLGNHKENLPLVIIRPTMITSTLFEPFPGWIEGLRTVDSVIIAYGKGVLKCFLVDV 325

Query: 303 -------PADMVVNAIIMAILTHKLQPSGRTIIYHIGSSMRNSMRIDTLFSYIHQYFTKK 362
                  PADMV NA+I A  TH    S   ++Y +GSS +N +    +   +  YFTK 
Sbjct: 326 NSVCDMIPADMVANAMIAAAATH-AGGSKVHMVYQVGSSHQNPIIYGEIREILFCYFTKN 385

Query: 363 PWMNEDGKVIKVKKITSFNNIASFHRYMTIHYVF-LKGFEFVNKAFCYSFQDKYNDLQRK 422
              + +G +I V K+     +A F  YMTI Y   ++  + V+  +     D+Y +  RK
Sbjct: 386 SLRSRNGSMITVSKMKLIPTLALFSLYMTIRYKLPVQLLKLVDIIYPSREGDEYKNKNRK 445

Query: 423 FNWVMRQVELYNSFIFFKAKFDDTNLKKL--RIVARDNGINPNTSL-LDPKDINWEDYFL 482
            + VMR V+LY  ++ FK  FDD N K L  +    DN  + N     DPK I W+DY +
Sbjct: 446 IDMVMRLVKLYEPYVLFKGIFDDRNTKNLCAKQKEEDNRNSENFMFDFDPKIIKWKDYLI 495

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93ZB91.1e-11946.60Fatty acyl-CoA reductase 3 OS=Arabidopsis thaliana OX=3702 GN=FAR3 PE=1 SV=1[more]
Q9XGY74.3e-11143.53Alcohol-forming fatty acyl-CoA reductase OS=Simmondsia chinensis OX=3999 PE=1 SV... [more]
Q391525.5e-10643.47Fatty acyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=FAR1 PE=1 SV=1[more]
Q9LXN39.6e-10342.39Probable fatty acyl-CoA reductase 4 OS=Arabidopsis thaliana OX=3702 GN=FAR4 PE=1... [more]
Q0WRB06.9e-10143.00Probable fatty acyl-CoA reductase 5 OS=Arabidopsis thaliana OX=3702 GN=FAR5 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1FYH09.3e-23492.51Fatty acyl-CoA reductase OS=Cucurbita moschata OX=3662 GN=LOC111448658 PE=3 SV=1[more]
A0A6J1FYH02.7e-13253.50Fatty acyl-CoA reductase OS=Cucurbita moschata OX=3662 GN=LOC111448658 PE=3 SV=1[more]
A0A6J1FTJ12.3e-20875.61Fatty acyl-CoA reductase OS=Cucurbita moschata OX=3662 GN=LOC111448659 PE=3 SV=1[more]
A0A6J1J9667.4e-20776.02Fatty acyl-CoA reductase OS=Cucurbita maxima OX=3661 GN=LOC111484588 PE=3 SV=1[more]
A0A6J1DGK92.0e-17563.67Fatty acyl-CoA reductase OS=Momordica charantia OX=3673 GN=LOC111020672 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
KAG6570487.16.4e-23793.14Fatty acyl-CoA reductase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6570487.11.2e-13154.42Fatty acyl-CoA reductase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022944110.15.6e-13253.50fatty acyl-CoA reductase 3-like [Cucurbita moschata][more]
XP_022985825.18.7e-21076.02fatty acyl-CoA reductase 3-like [Cucurbita maxima][more]
XP_022944111.14.8e-20875.61fatty acyl-CoA reductase 3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G33790.18.1e-12146.60Jojoba acyl CoA reductase-related male sterility protein [more]
AT5G22500.13.9e-10743.47fatty acid reductase 1 [more]
AT3G44540.16.9e-10442.39fatty acid reductase 4 [more]
AT3G44550.14.9e-10243.00fatty acid reductase 5 [more]
AT3G44560.11.1e-9640.97fatty acid reductase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 186..206
NoneNo IPR availableGENE3D3.40.50.720coord: 2..380
e-value: 3.1E-48
score: 166.8
NoneNo IPR availablePANTHERPTHR11011:SF99FATTY ACYL-COA REDUCTASE 3coord: 3..479
NoneNo IPR availableCDDcd05236FAR-N_SDR_ecoord: 3..354
e-value: 3.37793E-88
score: 272.634
IPR013120Fatty acyl-coenzyme A reductase, NAD-binding domainPFAMPF07993NAD_binding_4coord: 3..309
e-value: 1.2E-50
score: 172.1
IPR033640Fatty acyl-CoA reductase, C-terminalPFAMPF03015Sterilecoord: 384..482
e-value: 1.6E-11
score: 44.3
IPR026055Fatty acyl-CoA reductasePANTHERPTHR11011MALE STERILITY PROTEIN 2-RELATEDcoord: 3..479
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 4..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G001920.1CmoCh20G001920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0035336 long-chain fatty-acyl-CoA metabolic process
biological_process GO:0010345 suberin biosynthetic process
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0102965 alcohol-forming fatty acyl-CoA reductase activity
molecular_function GO:0080019 fatty-acyl-CoA reductase (alcohol-forming) activity