CmoCh20G000670 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G000670
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionLipoxygenase
LocationCmo_Chr20: 367654 .. 369552 (+)
RNA-Seq ExpressionCmoCh20G000670
SyntenyCmoCh20G000670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCAGGTTGTTGTTGTGAAAAGCTCGCAGTTTTCCCACTTCGATTTGTTCTTGTTTTCGTTGTAGGCTTAGAACTCATTGTTGTGTGGAACCTTACATCATCGCATCAAACAGACAACTGAGTGCAATGCATCCAATCTATAGACTCCTGCATCCTCATTTTCGATACACCATGGAGATCAACGCACTTGCTCGCCAAGCTCTCATCAATGCAAATGGGATCATTGAGACTTCCTTTTCACCTGGAAAGTACTCCATGGAATTTAGTTCTGTTGCTTACAAAGAGCAGTGGCAGTTCAACTTAGAAGCTTTGCCCGCCGACTTGATTCACAGGTGCTTATTCTTCAAATCTCTTTCATCTTAATCAACAAACCAACCAATTTGTAATCTCTTTCTCTTACTATCTTGTGTGTTGTTGTTGTTGTTGTTCTGGGAAGGGGGTTGGCAGTTGAGGACCCAAATGCACCACATGGTCTGAAGCTTACCATTGAAGACTACCCTTTTGCCAACGATGGCCTAATCCTTTGGGATGCCATCAAAGACTGGGCAACAGAATATGTGAACTACTACTATCCAGATCCAAGTCTGGTTAAGTCAGACGAGGAACTTCAAGCATGGTGGACGGAGATTCGAACAGAAGGGCATGCAGACAAGAAGGATGAACCATGGTGGCCAGTTCTCAACACTCCAGAAGACCTTATCAGTGTTGTCACCACCATCATGTGGGTAACCTCCGGGCATCACGCGGCAGTGAACTTTGGGCAATACTCGTTCGCTGGATATTTTCCAAATCGACCAAGTATTGCTAGAATCAATGTTCCCGTGGAAGAAAAAAACAAAGAGAAATGGGAGTACTTTCTTAACAAGCCAGAGGATGTGTTATTAGAAACCTTTCCTACACAGCTTCAAGCGACCAAAGTCACGGCCGTTTTGAACATACTGTCGAGTCATTCTCCAGATGAAGAGTACATGGGAAAAGATATGGAACAAGCATGGGCTGATGAGCCTTTTATAAAAGGAGCATTTGAGAAGTTCAGTGGGAGATTGAAGGAACTAGAAGGGATAATTGATAAAAGGAATGCCGACCCAAATCTAAAGAACAGACATGGAGCCGGGGTGACGCCTTATGAACTTTTGAAGCCTGAATCAGAGCCTGGAGTGACAGGAAAAGGAGTTCCTTACAGTATTTCCATCTAAAACCCCATGCCATAATGCTTTTTGTTTATTGAAATAAAGAGGTGATCGAGTTGCAACAATGTTGAATGTATCCAAACCACTTCACATTAAAATGAATAAACGTTGTTAAGATATAGAATACATCTGAATTTGAGTTTATTATTGAATAAGCATGAATGAATTAGAAGGTTGCGCTAATGAAGGAAGATGGGTCGGGGGGGAGGGAGACTTCAACAGCCCCTTCCAGCATCTGAACCACTTTGTTCATGGTGGGCCTTAGAGATGGTTCCTCTTGGATGCACCAGATTGCAATCATCACGAACCTCCTTACTCTCTTCAGGTCCACCTTCGCTTCCTCGTCATGCTCTACCAACATCTCCATCTTCCCGTCTCTGAAGCAGTCATAACTCCAATCTGCCAACACCATTCTATCTTCGTCCTCTGCTTTTTCCTCGAAGTTCCTTCTGCAGCATATAATCTCCAGCAACAATATCCCAAAACTGTAGACGTCCACCTTCACTGTGATGGGGAGGCTTCTAAACCACTCCGGAGCTACATATCCTTTGGTTCCTCTGATTGCCGTCAGTGTTCGAGTTTGATCCCTTTTCAGAAGCTTTGCCAAACCAAAGTCTGCAATTCGCGCGGTAAAGGAGTCGTCCAGAAGGATGTTTTGAGGCTTAATATCACAATGAATGGTCTGAGTACTGCACTCTTGGTGTAG

mRNA sequence

ATGGTCAGGCTTAGAACTCATTGTTGTGTGGAACCTTACATCATCGCATCAAACAGACAACTGAGTGCAATGCATCCAATCTATAGACTCCTGCATCCTCATTTTCGATACACCATGGAGATCAACGCACTTGCTCGCCAAGCTCTCATCAATGCAAATGGGATCATTGAGACTTCCTTTTCACCTGGAAAGTACTCCATGGAATTTAGTTCTGTTGCTTACAAAGAGCAGTGGCAGTTCAACTTAGAAGCTTTGCCCGCCGACTTGATTCACAGGGGGTTGGCAGTTGAGGACCCAAATGCACCACATGGTCTGAAGCTTACCATTGAAGACTACCCTTTTGCCAACGATGGCCTAATCCTTTGGGATGCCATCAAAGACTGGGCAACAGAATATGTGAACTACTACTATCCAGATCCAAGTCTGGTTAAGTCAGACGAGGAACTTCAAGCATGGTGGACGGAGATTCGAACAGAAGGGCATGCAGACAAGAAGGATGAACCATGGTGGCCAGTTCTCAACACTCCAGAAGACCTTATCAGTGTTGTCACCACCATCATGTGGGTAACCTCCGGGCATCACGCGGCAGTGAACTTTGGGCAATACTCGTTCGCTGGATATTTTCCAAATCGACCAAGTATTGCTAGAATCAATGTTCCCGTGGAAGAAAAAAACAAAGAGAAATGGGAGTACTTTCTTAACAAGCCAGAGGATGTGTTATTAGAAACCTTTCCTACACAGCTTCAAGCGACCAAAGTCACGGCCGTTTTGAACATACTGTCGAGTCATTCTCCAGATGAAGAGTACATGGGAAAAGATATGGAACAAGCATGGGCTGATGAGCCTTTTATAAAAGGAGCATTTGAGAAGTTCAGTGGGAGATTGAAGGAACTAGAAGGGATAATTGATAAAAGGAATGCCGACCCAAATCTAAAGAACAGACATGGAGCCGGGGTGACGCCTTATGAACTTTTGAAGCCTGAATCAGAGCCTGGAGTGACAGGAAAAGGAGTTCCTTACACATATAATCTCCAGCAACAATATCCCAAAACTGTAGACGTCCACCTTCACTGTGATGGGGAGGCTTCTAAACCACTCCGGAGCTACATATCCTTTGGTTCCTCTGATTGCCGTCAGTGTTCGAGTTTGATCCCTTTTCAGAAGCTTTGCCAAACCAAAGTCTGCAATTCGCGCGGTAAAGGAGTCGTCCAGAAGGATGTTTTGAGGCTTAATATCACAATGAATGGTCTGAGTACTGCACTCTTGGTGTAG

Coding sequence (CDS)

ATGGTCAGGCTTAGAACTCATTGTTGTGTGGAACCTTACATCATCGCATCAAACAGACAACTGAGTGCAATGCATCCAATCTATAGACTCCTGCATCCTCATTTTCGATACACCATGGAGATCAACGCACTTGCTCGCCAAGCTCTCATCAATGCAAATGGGATCATTGAGACTTCCTTTTCACCTGGAAAGTACTCCATGGAATTTAGTTCTGTTGCTTACAAAGAGCAGTGGCAGTTCAACTTAGAAGCTTTGCCCGCCGACTTGATTCACAGGGGGTTGGCAGTTGAGGACCCAAATGCACCACATGGTCTGAAGCTTACCATTGAAGACTACCCTTTTGCCAACGATGGCCTAATCCTTTGGGATGCCATCAAAGACTGGGCAACAGAATATGTGAACTACTACTATCCAGATCCAAGTCTGGTTAAGTCAGACGAGGAACTTCAAGCATGGTGGACGGAGATTCGAACAGAAGGGCATGCAGACAAGAAGGATGAACCATGGTGGCCAGTTCTCAACACTCCAGAAGACCTTATCAGTGTTGTCACCACCATCATGTGGGTAACCTCCGGGCATCACGCGGCAGTGAACTTTGGGCAATACTCGTTCGCTGGATATTTTCCAAATCGACCAAGTATTGCTAGAATCAATGTTCCCGTGGAAGAAAAAAACAAAGAGAAATGGGAGTACTTTCTTAACAAGCCAGAGGATGTGTTATTAGAAACCTTTCCTACACAGCTTCAAGCGACCAAAGTCACGGCCGTTTTGAACATACTGTCGAGTCATTCTCCAGATGAAGAGTACATGGGAAAAGATATGGAACAAGCATGGGCTGATGAGCCTTTTATAAAAGGAGCATTTGAGAAGTTCAGTGGGAGATTGAAGGAACTAGAAGGGATAATTGATAAAAGGAATGCCGACCCAAATCTAAAGAACAGACATGGAGCCGGGGTGACGCCTTATGAACTTTTGAAGCCTGAATCAGAGCCTGGAGTGACAGGAAAAGGAGTTCCTTACACATATAATCTCCAGCAACAATATCCCAAAACTGTAGACGTCCACCTTCACTGTGATGGGGAGGCTTCTAAACCACTCCGGAGCTACATATCCTTTGGTTCCTCTGATTGCCGTCAGTGTTCGAGTTTGATCCCTTTTCAGAAGCTTTGCCAAACCAAAGTCTGCAATTCGCGCGGTAAAGGAGTCGTCCAGAAGGATGTTTTGAGGCTTAATATCACAATGAATGGTCTGAGTACTGCACTCTTGGTGTAG

Protein sequence

MVRLRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPGKYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWDAIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVVTTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLETFPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIIDKRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNLQQQYPKTVDVHLHCDGEASKPLRSYISFGSSDCRQCSSLIPFQKLCQTKVCNSRGKGVVQKDVLRLNITMNGLSTALLV
Homology
BLAST of CmoCh20G000670 vs. ExPASy Swiss-Prot
Match: O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.7e-155
Identity = 245/341 (71.85%), Postives = 293/341 (85.92%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCC EPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALAR+ALINANG+IE+SF PG
Sbjct: 559 LRTHCCTEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALAREALINANGVIESSFFPG 618

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KY++E SS+AY  +W+F+ EALP +LI RGLAVEDPN PHGLKL IEDYPFANDGL+LWD
Sbjct: 619 KYAIELSSIAYGAEWRFDQEALPQNLISRGLAVEDPNEPHGLKLAIEDYPFANDGLVLWD 678

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
            +K W T YVN+YYP  +L++SD+ELQAWW+EI+  GH DK+DEPWWP L TP DLI ++
Sbjct: 679 ILKQWVTNYVNHYYPQTNLIESDKELQAWWSEIKNVGHGDKRDEPWWPELKTPNDLIGII 738

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTI+WVTSGHHAAVNFGQYS+AGYFPNRP++AR  +P E+   E+WE+F+NKPE+ LL  
Sbjct: 739 TTIVWVTSGHHAAVNFGQYSYAGYFPNRPTVARSKMPTEDPTAEEWEWFMNKPEEALLRC 798

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FP+Q+QATKV A+L++LS+HSPDEEY+G+ +E  WA++P I  AFE FSG+LKELEGIID
Sbjct: 799 FPSQIQATKVMAILDVLSNHSPDEEYIGEKIEPYWAEDPVINAAFEVFSGKLKELEGIID 858

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
            RN D  L NR+GAGV PYELLKP SEPGVTGKGVPY+ ++
Sbjct: 859 ARNNDSKLSNRNGAGVMPYELLKPYSEPGVTGKGVPYSISI 899

BLAST of CmoCh20G000670 vs. ExPASy Swiss-Prot
Match: R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.0e-147
Identity = 244/341 (71.55%), Postives = 284/341 (83.28%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHC  EPYIIA+NRQLS MHPI RLL PH RYTM+IN LAR +LINANGIIE+SFSP 
Sbjct: 559 LRTHCVTEPYIIATNRQLSKMHPIQRLLCPHLRYTMQINGLARLSLINANGIIESSFSPR 618

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYSM+ SS AY ++W+F+ EALPADLI RG+AVED +APHG+KLTIEDYPFANDGL+LWD
Sbjct: 619 KYSMQLSSDAYAQKWRFDHEALPADLISRGMAVEDESAPHGIKLTIEDYPFANDGLLLWD 678

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIK WAT Y+N+YYP   LV+SDEELQAWWTEIRT GHADKKDEPWWP L T +DLI VV
Sbjct: 679 AIKQWATAYINHYYPQAKLVESDEELQAWWTEIRTVGHADKKDEPWWPQLKTQQDLIGVV 738

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           +TIMWV+SGHH+AVNFGQY F GYFPNRP+IAR  +P E+   E+WE F+ KPEDVLL  
Sbjct: 739 STIMWVSSGHHSAVNFGQYDFGGYFPNRPTIARTKMPNEDPTFEEWEAFMEKPEDVLLNC 798

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQ+QATKV A+L++LSSHSPDEEY+G  ME +W  EP IK AFE+F GRLK+L+ IID
Sbjct: 799 FPTQIQATKVMAILDVLSSHSPDEEYIGTSMEASWEAEPAIKSAFEEFCGRLKKLDDIID 858

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
            RN DP L+NR GAG+  Y+LLKP S  GVTGKGVPY+ ++
Sbjct: 859 SRNRDPILRNRTGAGLVQYQLLKPFSGHGVTGKGVPYSISI 899

BLAST of CmoCh20G000670 vs. ExPASy Swiss-Prot
Match: P38418 (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.3e-139
Identity = 228/341 (66.86%), Postives = 269/341 (78.89%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTH C EPYIIA+NRQLSAMHPIYRLLHPHFRYTMEINA ARQ+L+N  GIIET F PG
Sbjct: 556 LRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPG 615

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KY++E SS  Y + W+F+ E LPADLI RGLA ED  A HG++LTI DYPFANDGLILWD
Sbjct: 616 KYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPFANDGLILWD 675

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIK+W T+YV +YYPD  L+ SDEELQ WW+E+R  GH DKKDEPWWPVL T +DLI VV
Sbjct: 676 AIKEWVTDYVKHYYPDEELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVV 735

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTI WVTSGHHAAVNFGQY + GYFPNRP+  RI +P E+   E  + F   PE VLL+T
Sbjct: 736 TTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKT 795

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           +P+Q QAT V   L++LS+HSPDEEY+G+  E +WA+EP I  AFE+F G+L+ LEG+ID
Sbjct: 796 YPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVID 855

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           +RN +  LKNR GAGV  YELLKP SE GVTG GVPY+ ++
Sbjct: 856 ERNVNITLKNRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896

BLAST of CmoCh20G000670 vs. ExPASy Swiss-Prot
Match: Q8GSM2 (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 3.6e-137
Identity = 226/341 (66.28%), Postives = 277/341 (81.23%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTH CVEPYIIA+NRQLS MHP+YRLLHPHFRYTMEINALAR+ALINA+GIIE +F  G
Sbjct: 558 LRTHACVEPYIIATNRQLSRMHPVYRLLHPHFRYTMEINALAREALINADGIIEEAFLAG 617

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYS+E SSVAY   WQFN EALP DLI+RGLAV   +    L+L I+DYP+A+DGL++W 
Sbjct: 618 KYSIELSSVAYGAAWQFNTEALPEDLINRGLAVRRDDGE--LELAIKDYPYADDGLLIWG 677

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           +IK WA++YV++YY     V  DEEL+AWW E+RT+GHADKKDEPWWPV +T E+L+ ++
Sbjct: 678 SIKQWASDYVDFYYKSDGDVAGDEELRAWWEEVRTKGHADKKDEPWWPVCDTKENLVQIL 737

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           T IMWVTSGHHAAVNFGQY +AGYFPNRP++ R N+PVEE   ++ + F+ +PE+VLL++
Sbjct: 738 TIIMWVTSGHHAAVNFGQYHYAGYFPNRPTVVRRNIPVEENRDDEMKKFMARPEEVLLQS 797

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
            P+Q+QA KV A L+ILSSHSPDEEYMG+  E AW  EP +K AFEKFSGRLKE EG ID
Sbjct: 798 LPSQMQAIKVMATLDILSSHSPDEEYMGEYAEPAWLAEPMVKAAFEKFSGRLKEAEGTID 857

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
            RN +P  KNR GAG+ PYELLKP SEPGVTG+G+P + ++
Sbjct: 858 MRNNNPENKNRCGAGIVPYELLKPFSEPGVTGRGIPNSISI 896

BLAST of CmoCh20G000670 vs. ExPASy Swiss-Prot
Match: Q84YK8 (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 6.2e-129
Identity = 216/337 (64.09%), Postives = 261/337 (77.45%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHC VEPYIIA+NRQLS MHPIY+LLHPHFRYTM INALAR  LI+A GIIE SFSP 
Sbjct: 600 LRTHCAVEPYIIAANRQLSEMHPIYQLLHPHFRYTMRINALARSRLISAAGIIELSFSPQ 659

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYSME SSVAY + W+F++EALPADL+ RG+A EDP A HGL+L IEDYPFANDGL++WD
Sbjct: 660 KYSMELSSVAYDKLWRFDMEALPADLVRRGMAEEDPTAEHGLRLAIEDYPFANDGLLIWD 719

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIK W   YV  +YPD   V  DEELQA+WTE+RT+GH DKKD PWWP L++PE L   +
Sbjct: 720 AIKTWVQAYVARFYPDADSVAGDEELQAFWTEVRTKGHGDKKDAPWWPKLDSPESLAHTL 779

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEK-NKEKWEYFLNKPEDVLLE 243
           TTI+WV + HHAAVNFGQY F GYFPNRPSIAR  +PVEE  +    E FL+ P+  L E
Sbjct: 780 TTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGAAMERFLDNPDQALRE 839

Query: 244 TFPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGII 303
            FP+Q+QAT V AVL++LS+HS DEEY+G +  + W  +  ++ A+  F+ RLKE+EG+I
Sbjct: 840 CFPSQVQATVVMAVLDVLSTHSTDEEYLGGEQTRPWNSDAAVQAAYAGFTARLKEIEGVI 899

Query: 304 DKRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVP 340
           D RN D  LKNR GAG+ PY+L+KP S+ GVTG G+P
Sbjct: 900 DGRNKDRKLKNRCGAGILPYQLMKPFSDAGVTGMGIP 936

BLAST of CmoCh20G000670 vs. ExPASy TrEMBL
Match: A0A6J1FWY9 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448227 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.8e-201
Identity = 337/341 (98.83%), Postives = 340/341 (99.71%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG
Sbjct: 562 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 621

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD
Sbjct: 622 KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 681

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV
Sbjct: 682 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 741

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET
Sbjct: 742 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 801

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID
Sbjct: 802 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 861

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPY+ ++
Sbjct: 862 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYSISI 902

BLAST of CmoCh20G000670 vs. ExPASy TrEMBL
Match: A0A6J1JDX0 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483582 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.0e-195
Identity = 327/341 (95.89%), Postives = 337/341 (98.83%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG
Sbjct: 562 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 621

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYSMEFSSVAYKEQWQFNLEALPADLI+RGLAVEDPNAPHGLKLTIEDYPFANDGLILWD
Sbjct: 622 KYSMEFSSVAYKEQWQFNLEALPADLINRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 681

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLIS+V
Sbjct: 682 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISIV 741

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           +TIMWVTSGHHAAVNFGQYSF GYFPNRPSIARINVPVEEKNKEKWEYF+ KPE+VLLET
Sbjct: 742 STIMWVTSGHHAAVNFGQYSFGGYFPNRPSIARINVPVEEKNKEKWEYFVKKPENVLLET 801

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID
Sbjct: 802 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 861

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           KRNADP+LKNR+GAGV PYELLKPESEPGVTGKGVPY+ ++
Sbjct: 862 KRNADPSLKNRYGAGVAPYELLKPESEPGVTGKGVPYSISI 902

BLAST of CmoCh20G000670 vs. ExPASy TrEMBL
Match: A0A5D3BB51 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001440 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.8e-184
Identity = 307/341 (90.03%), Postives = 327/341 (95.89%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQ+LINA+GIIE+ FSPG
Sbjct: 449 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQSLINADGIIESCFSPG 508

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYS+EFSSVAYK QWQFNLEALPADLI+RGLAVEDPNAPHGLKL IEDYPFANDGLILWD
Sbjct: 509 KYSVEFSSVAYKAQWQFNLEALPADLINRGLAVEDPNAPHGLKLAIEDYPFANDGLILWD 568

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIK+WATEYVNYYYPDPSLVK+DEELQAWW EIRTEGHADKKDEPWWPVLNTPEDLI++V
Sbjct: 569 AIKEWATEYVNYYYPDPSLVKADEELQAWWNEIRTEGHADKKDEPWWPVLNTPEDLINIV 628

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTIMWV SGHHAAVNFGQYSFAGYFPNRPSIARINVP+E+ N+EKWE F+NKPE VLLET
Sbjct: 629 TTIMWVPSGHHAAVNFGQYSFAGYFPNRPSIARINVPLEDVNEEKWENFINKPEHVLLET 688

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKD+E AWADEPFIKGAFEKFSG+LKELEGIID
Sbjct: 689 FPTQLQATKVTAVLNILSSHSPDEEYMGKDIEAAWADEPFIKGAFEKFSGKLKELEGIID 748

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           +RNAD NLKNRHGAGV PY LLKPES PGVTG+GVPY+ ++
Sbjct: 749 ERNADRNLKNRHGAGVAPYRLLKPESGPGVTGQGVPYSISI 789

BLAST of CmoCh20G000670 vs. ExPASy TrEMBL
Match: A0A125S6K7 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=LOX10 PE=2 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.8e-184
Identity = 307/341 (90.03%), Postives = 327/341 (95.89%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQ+LINA+GIIE+ FSPG
Sbjct: 562 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQSLINADGIIESCFSPG 621

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYS+EFSSVAYK QWQFNLEALPADLI+RGLAVEDPNAPHGLKL IEDYPFANDGLILWD
Sbjct: 622 KYSVEFSSVAYKAQWQFNLEALPADLINRGLAVEDPNAPHGLKLAIEDYPFANDGLILWD 681

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIK+WATEYVNYYYPDPSLVK+DEELQAWW EIRTEGHADKKDEPWWPVLNTPEDLI++V
Sbjct: 682 AIKEWATEYVNYYYPDPSLVKADEELQAWWNEIRTEGHADKKDEPWWPVLNTPEDLINIV 741

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTIMWV SGHHAAVNFGQYSFAGYFPNRPSIARINVP+E+ N+EKWE F+NKPE VLLET
Sbjct: 742 TTIMWVPSGHHAAVNFGQYSFAGYFPNRPSIARINVPLEDVNEEKWENFINKPEHVLLET 801

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKD+E AWADEPFIKGAFEKFSG+LKELEGIID
Sbjct: 802 FPTQLQATKVTAVLNILSSHSPDEEYMGKDIEAAWADEPFIKGAFEKFSGKLKELEGIID 861

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           +RNAD NLKNRHGAGV PY LLKPES PGVTG+GVPY+ ++
Sbjct: 862 ERNADRNLKNRHGAGVAPYRLLKPESGPGVTGQGVPYSISI 902

BLAST of CmoCh20G000670 vs. ExPASy TrEMBL
Match: A0A5A7TJ36 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00240 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.8e-184
Identity = 307/341 (90.03%), Postives = 327/341 (95.89%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQ+LINA+GIIE+ FSPG
Sbjct: 449 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQSLINADGIIESCFSPG 508

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYS+EFSSVAYK QWQFNLEALPADLI+RGLAVEDPNAPHGLKL IEDYPFANDGLILWD
Sbjct: 509 KYSVEFSSVAYKAQWQFNLEALPADLINRGLAVEDPNAPHGLKLAIEDYPFANDGLILWD 568

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIK+WATEYVNYYYPDPSLVK+DEELQAWW EIRTEGHADKKDEPWWPVLNTPEDLI++V
Sbjct: 569 AIKEWATEYVNYYYPDPSLVKADEELQAWWNEIRTEGHADKKDEPWWPVLNTPEDLINIV 628

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTIMWV SGHHAAVNFGQYSFAGYFPNRPSIARINVP+E+ N+EKWE F+NKPE VLLET
Sbjct: 629 TTIMWVPSGHHAAVNFGQYSFAGYFPNRPSIARINVPLEDVNEEKWEKFINKPEHVLLET 688

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKD+E AWADEPFIKGAFEKFSG+LKELEGIID
Sbjct: 689 FPTQLQATKVTAVLNILSSHSPDEEYMGKDIEAAWADEPFIKGAFEKFSGKLKELEGIID 748

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           +RNAD NLKNRHGAGV PY LLKPES PGVTG+GVPY+ ++
Sbjct: 749 ERNADRNLKNRHGAGVAPYRLLKPESGPGVTGQGVPYSISI 789

BLAST of CmoCh20G000670 vs. NCBI nr
Match: KAG6570374.1 (Linoleate 13S-lipoxygenase 2-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 710.3 bits (1832), Expect = 1.0e-200
Identity = 337/341 (98.83%), Postives = 340/341 (99.71%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG
Sbjct: 562 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 621

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD
Sbjct: 622 KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 681

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV
Sbjct: 682 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 741

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET
Sbjct: 742 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 801

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID
Sbjct: 802 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 861

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPY+ ++
Sbjct: 862 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYSISI 902

BLAST of CmoCh20G000670 vs. NCBI nr
Match: XP_022943468.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 710.3 bits (1832), Expect = 1.0e-200
Identity = 337/341 (98.83%), Postives = 340/341 (99.71%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG
Sbjct: 562 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 621

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD
Sbjct: 622 KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 681

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV
Sbjct: 682 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 741

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET
Sbjct: 742 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 801

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID
Sbjct: 802 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 861

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPY+ ++
Sbjct: 862 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYSISI 902

BLAST of CmoCh20G000670 vs. NCBI nr
Match: XP_023511795.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 698.0 bits (1800), Expect = 5.1e-197
Identity = 331/341 (97.07%), Postives = 337/341 (98.83%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG
Sbjct: 558 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 617

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYSMEFSSVAYKEQWQFNLEALPADLI+RGLAVEDPNAPHGLKLTIEDYPFANDGLILWD
Sbjct: 618 KYSMEFSSVAYKEQWQFNLEALPADLINRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 677

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLIS+V
Sbjct: 678 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISIV 737

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
            TIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWE FLNKPE+VLLET
Sbjct: 738 ATIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWECFLNKPENVLLET 797

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID
Sbjct: 798 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 857

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           KRNADPNLKNRHGAGV PYELLKPESEPGVTGKGVPY+ ++
Sbjct: 858 KRNADPNLKNRHGAGVAPYELLKPESEPGVTGKGVPYSISI 898

BLAST of CmoCh20G000670 vs. NCBI nr
Match: XP_022985543.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 692.6 bits (1786), Expect = 2.1e-195
Identity = 327/341 (95.89%), Postives = 337/341 (98.83%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG
Sbjct: 562 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 621

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYSMEFSSVAYKEQWQFNLEALPADLI+RGLAVEDPNAPHGLKLTIEDYPFANDGLILWD
Sbjct: 622 KYSMEFSSVAYKEQWQFNLEALPADLINRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 681

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLIS+V
Sbjct: 682 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISIV 741

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           +TIMWVTSGHHAAVNFGQYSF GYFPNRPSIARINVPVEEKNKEKWEYF+ KPE+VLLET
Sbjct: 742 STIMWVTSGHHAAVNFGQYSFGGYFPNRPSIARINVPVEEKNKEKWEYFVKKPENVLLET 801

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID
Sbjct: 802 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 861

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           KRNADP+LKNR+GAGV PYELLKPESEPGVTGKGVPY+ ++
Sbjct: 862 KRNADPSLKNRYGAGVAPYELLKPESEPGVTGKGVPYSISI 902

BLAST of CmoCh20G000670 vs. NCBI nr
Match: KAA0041379.1 (linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 655.2 bits (1689), Expect = 3.8e-184
Identity = 307/341 (90.03%), Postives = 327/341 (95.89%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQ+LINA+GIIE+ FSPG
Sbjct: 449 LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQSLINADGIIESCFSPG 508

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KYS+EFSSVAYK QWQFNLEALPADLI+RGLAVEDPNAPHGLKL IEDYPFANDGLILWD
Sbjct: 509 KYSVEFSSVAYKAQWQFNLEALPADLINRGLAVEDPNAPHGLKLAIEDYPFANDGLILWD 568

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIK+WATEYVNYYYPDPSLVK+DEELQAWW EIRTEGHADKKDEPWWPVLNTPEDLI++V
Sbjct: 569 AIKEWATEYVNYYYPDPSLVKADEELQAWWNEIRTEGHADKKDEPWWPVLNTPEDLINIV 628

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTIMWV SGHHAAVNFGQYSFAGYFPNRPSIARINVP+E+ N+EKWE F+NKPE VLLET
Sbjct: 629 TTIMWVPSGHHAAVNFGQYSFAGYFPNRPSIARINVPLEDVNEEKWEKFINKPEHVLLET 688

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           FPTQLQATKVTAVLNILSSHSPDEEYMGKD+E AWADEPFIKGAFEKFSG+LKELEGIID
Sbjct: 689 FPTQLQATKVTAVLNILSSHSPDEEYMGKDIEAAWADEPFIKGAFEKFSGKLKELEGIID 748

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           +RNAD NLKNRHGAGV PY LLKPES PGVTG+GVPY+ ++
Sbjct: 749 ERNADRNLKNRHGAGVAPYRLLKPESGPGVTGQGVPYSISI 789

BLAST of CmoCh20G000670 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 496.9 bits (1278), Expect = 1.6e-140
Identity = 228/341 (66.86%), Postives = 269/341 (78.89%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTH C EPYIIA+NRQLSAMHPIYRLLHPHFRYTMEINA ARQ+L+N  GIIET F PG
Sbjct: 556 LRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPG 615

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KY++E SS  Y + W+F+ E LPADLI RGLA ED  A HG++LTI DYPFANDGLILWD
Sbjct: 616 KYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPFANDGLILWD 675

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIK+W T+YV +YYPD  L+ SDEELQ WW+E+R  GH DKKDEPWWPVL T +DLI VV
Sbjct: 676 AIKEWVTDYVKHYYPDEELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVV 735

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTI WVTSGHHAAVNFGQY + GYFPNRP+  RI +P E+   E  + F   PE VLL+T
Sbjct: 736 TTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKT 795

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
           +P+Q QAT V   L++LS+HSPDEEY+G+  E +WA+EP I  AFE+F G+L+ LEG+ID
Sbjct: 796 YPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVID 855

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           +RN +  LKNR GAGV  YELLKP SE GVTG GVPY+ ++
Sbjct: 856 ERNVNITLKNRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896

BLAST of CmoCh20G000670 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 404.8 bits (1039), Expect = 8.3e-113
Identity = 187/343 (54.52%), Postives = 249/343 (72.59%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTH  +EPYIIA+NRQLS MHP+Y+LLHPH RYT+EINA AR++LIN  GIIE+ F+PG
Sbjct: 577 LRTHASMEPYIIATNRQLSTMHPVYKLLHPHMRYTLEINARARKSLINGGGIIESCFTPG 636

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KY+ME SS AYK  W+F++E LPADL+ RG+A ED +A  G++L I+DYP+A DGL++W 
Sbjct: 637 KYAMELSSAAYKSMWRFDMEGLPADLVRRGMAEEDSSAECGVRLVIDDYPYAADGLLIWK 696

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AIKD    YV ++Y D   + SD ELQAWW EI+ +GH DKKDEPWWP LNT +DL  ++
Sbjct: 697 AIKDLVESYVKHFYSDSKSITSDLELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQIL 756

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           T ++W+ SG HAA+NFGQY F GY PNRP++ R  +P  ++    +E F+  P+   L +
Sbjct: 757 TNMIWIASGQHAAINFGQYPFGGYVPNRPTLLRKLIP--QETDPDYEMFMRNPQYSFLGS 816

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYM--GKDMEQAWADEPFIKGAFEKFSGRLKELEGI 303
            PTQLQATKV AV   LS+HSPDEEY+   +++++ W  +  +   F KFS  L ++E  
Sbjct: 817 LPTQLQATKVMAVQETLSTHSPDEEYLIELREVQRHWFQDEQVVKYFNKFSEELVKIEKT 876

Query: 304 IDKRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           I++RN D  LKNR GAG+ PYELL P S  GVTG+G+P + ++
Sbjct: 877 INERNKDKKLKNRTGAGMPPYELLLPTSPHGVTGRGIPNSISI 917

BLAST of CmoCh20G000670 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.1e-111
Identity = 183/342 (53.51%), Postives = 251/342 (73.39%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTH C+EP+I+A++RQLSAMHPI++LL PH RYT+EINA+ARQ LI+A+G+IE+ F+ G
Sbjct: 587 LRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEINAVARQTLISADGVIESCFTAG 646

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           +Y +E SS AYK +W+F++E LPADLI RG+AV DP  PHGLKL +EDYP+ANDGL+LW 
Sbjct: 647 QYGLEISSAAYKNKWRFDMEGLPADLIRRGMAVPDPTQPHGLKLLVEDYPYANDGLLLWS 706

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AI+ W   YV  YY + +L+++D ELQAW++E    GHAD +D  WWP L+T EDL+SV+
Sbjct: 707 AIQTWVRTYVERYYANSNLIQTDTELQAWYSESINVGHADHRDAEWWPKLSTVEDLVSVI 766

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TTI+W+ S  HAA+NFGQY + GY PNRP + R  +P  +++  ++  F+  P+     +
Sbjct: 767 TTIIWLASAQHAALNFGQYPYGGYVPNRPPLMRRLIP--DESDPEFTSFIEDPQKYFFSS 826

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQA-WADEPFIKGAFEKFSGRLKELEGII 303
            P+ LQ TK  AV++ LS+HSPDEEY+G+  + + W  +  I  AF  FS  +  +E  I
Sbjct: 827 MPSLLQTTKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVDAFYGFSAEIGRIEKEI 886

Query: 304 DKRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           DKRN DP+ +NR GAGV PYEL+ P SEPGVT +GVP + ++
Sbjct: 887 DKRNRDPSRRNRCGAGVLPYELMAPSSEPGVTCRGVPNSVSI 926

BLAST of CmoCh20G000670 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 397.5 bits (1020), Expect = 1.3e-110
Identity = 181/342 (52.92%), Postives = 251/342 (73.39%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           LRTH C+EP+I+A++RQLSAMHPI++LL PH RYT+EINALARQ+LI+A+G+IE  F+ G
Sbjct: 580 LRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLISADGVIEGGFTAG 639

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
            Y ME S+ AYK  W+F++E LPADLI RG+A+ D   PHGLKL IEDYP+ANDGL+LW 
Sbjct: 640 AYGMEMSAAAYKSSWRFDMEGLPADLIRRGMAIPDATQPHGLKLLIEDYPYANDGLLLWS 699

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AI+ W   YV  YYP+P+L+K+D ELQ+W++E    GHAD +D  WWP L+T +DL+S++
Sbjct: 700 AIQTWVRTYVERYYPNPNLIKTDSELQSWYSESINVGHADLRDADWWPELSTVDDLVSIL 759

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           TT++W+ S  HAA+NFGQY + GY PNRP + R  +P  +++  ++  F++ PE     +
Sbjct: 760 TTLIWLASAQHAALNFGQYPYGGYVPNRPPLMRRLIP--DESDPEYASFISHPEKYYFSS 819

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQA-WADEPFIKGAFEKFSGRLKELEGII 303
            P+  Q +K  AV++ LS+HSPDEEY+G+  + + W  +  I  AF  F+  +  +E  I
Sbjct: 820 MPSLAQTSKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVEAFYGFAAEIGRIEKEI 879

Query: 304 DKRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           +KRNADP+ +NR GAGV PYELL P SEPGVT +GVP + ++
Sbjct: 880 EKRNADPDRRNRCGAGVLPYELLVPSSEPGVTCRGVPNSVSI 919

BLAST of CmoCh20G000670 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 369.0 bits (946), Expect = 5.1e-102
Identity = 177/341 (51.91%), Postives = 231/341 (67.74%), Query Frame = 0

Query: 4   LRTHCCVEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPG 63
           ++TH  +EP++IA+NRQLS +HP+++LL PHFR TM INALARQ LIN  GI E +  P 
Sbjct: 521 MQTHASIEPFVIATNRQLSVLHPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPS 580

Query: 64  KYSMEFSSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWD 123
           KY+ME SS  YK  W F  +ALPA+L  RG+AVEDP APHGL+L I+DYP+A DGL +W 
Sbjct: 581 KYAMEMSSFIYKNHWTFPDQALPAELKKRGMAVEDPEAPHGLRLRIKDYPYAVDGLEVWY 640

Query: 124 AIKDWATEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVV 183
           AI+ W  +Y+  +Y     +++D ELQAWW E+R EGH DKK EPWWP + T E+L+   
Sbjct: 641 AIESWVRDYIFLFYKIEEDIQTDTELQAWWKEVREEGHGDKKSEPWWPKMQTREELVESC 700

Query: 184 TTIMWVTSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLET 243
           T I+WV S  HAAVNFGQY  AGY PNRP+I+R  +P E  N  ++E     P+ V L+T
Sbjct: 701 TIIIWVASALHAAVNFGQYPVAGYLPNRPTISRQYMPKE--NTPEFEELEKNPDKVFLKT 760

Query: 244 FPTQLQATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIID 303
              QLQ     +++ ILS+HS DE Y+G+   + WA E     AFEKF  ++KE+E  ID
Sbjct: 761 ITAQLQTLLGISLIEILSTHSSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNID 820

Query: 304 KRNADPNLKNRHGAGVTPYELLKPESEPGVTGKGVPYTYNL 345
           +RN D  LKNR G    PY LL P SE GVTG+G+P + ++
Sbjct: 821 ERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O243701.7e-15571.85Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO... [more]
R9WS043.0e-14771.55Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=... [more]
P384182.3e-13966.86Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1[more]
Q8GSM23.6e-13766.28Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1[more]
Q84YK86.2e-12964.09Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FWY94.8e-20198.83Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448227 PE=3 SV=1[more]
A0A6J1JDX01.0e-19595.89Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483582 PE=3 SV=1[more]
A0A5D3BB511.8e-18490.03Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001440 ... [more]
A0A125S6K71.8e-18490.03Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=LOX10 PE=2 SV=1[more]
A0A5A7TJ361.8e-18490.03Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00240 P... [more]
Match NameE-valueIdentityDescription
KAG6570374.11.0e-20098.83Linoleate 13S-lipoxygenase 2-1, chloroplastic, partial [Cucurbita argyrosperma s... [more]
XP_022943468.11.0e-20098.83linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata][more]
XP_023511795.15.1e-19797.07linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022985543.12.1e-19595.89linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita maxima][more]
KAA0041379.13.8e-18490.03linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G45140.11.6e-14066.86lipoxygenase 2 [more]
AT1G67560.18.3e-11354.52PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G72520.12.1e-11153.51PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G17420.11.3e-11052.92lipoxygenase 3 [more]
AT1G55020.15.1e-10251.91lipoxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013819Lipoxygenase, C-terminalPFAMPF00305Lipoxygenasecoord: 4..327
e-value: 2.0E-167
score: 558.7
IPR013819Lipoxygenase, C-terminalPROSITEPS51393LIPOXYGENASE_3coord: 1..344
score: 153.381439
NoneNo IPR availableGENE3D1.20.245.10coord: 2..342
e-value: 2.0E-137
score: 460.1
NoneNo IPR availablePANTHERPTHR11771:SF89LINOLEATE 13S-LIPOXYGENASE 2-1, CHLOROPLASTICcoord: 4..341
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 4..341
IPR020834Lipoxygenase, conserved sitePROSITEPS00081LIPOXYGENASE_2coord: 24..34
IPR036226Lipoxigenase, C-terminal domain superfamilySUPERFAMILY48484Lipoxigenasecoord: 4..340

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G000670.1CmoCh20G000670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0034440 lipid oxidation
biological_process GO:0031408 oxylipin biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen