CmoCh20G000050 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh20G000050
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	RING-type E3 ubiquitin transferase
Location	Cmo_Chr20: 97838 .. 98947 (+)
RNA-Seq Expression	CmoCh20G000050
Synteny	CmoCh20G000050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGAGTCAATTGCAACAAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCTTTCTGCCGTAGTGGCTTTGTTGAAGAGATGGGTAACGGTATGTCCAGCGACAATAATGGTAATAATAATAATAATAATAGTAATAATGCGCTTGAATCCGATTTTGGATCGGAGCGAGCTTTGTCCCTCTGGGCCCCGATACTGCTGGGTATGATGGGAAATCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTTGACGAAGATGATGATGATAATAATGATCAGGAGCATGGGGATGGGGAGGGGAACCGTGTGAGTAGTGAGCCGACTGAGCTTGATTCGGTCATTAGGAGGAGGAGGAGAAGTTCGGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGGTGAATCAGAGAATAGTTACGAGGGAGAGAGGAGCAGAGAAAGAGAAAGAGAACGAGAACGAGAAAGAGAGCGGGTTATCCTTATAAATCCTTTCAACCAGACCATTGTAGTTCAAGGAGGTGAGAGTCAAAATCATAACGCTATAGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCCCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTGAGAGTTGAATGCGGGGATAATCGTCTGCAATGTTCGGTGTGTTTGGATGAGTTTGAGATGGATGAAGAAGCCAAGGAGATGCCTTGTAGGCATAAGTTTCATTCAGGGTGTATTCTGCCGTGGCTGGAGCTGCATAGTTCCTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAGTCCAAGCGTGATGCGGATGGTGGGTCGGCTAGGTTTATGGGTAGTGGCAGTGGCAGTGGCAGTGGCAGTGGGGAGATTGATGGGAGAAACGCATCTGGGACTGGGAGAAGGTTTTCGTTTCCATGGCCATTCAATGGCTTGTTTTCTTCGCAAGGAAACTCCTCGGGAGGAAGTGGAAGTGAGTCTCAAAGGGATCACACATCCATTTGA

mRNA sequence

ATGGACGAGTCAATTGCAACAAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCTTTCTGCCGTAGTGGCTTTGTTGAAGAGATGGGTAACGGTATGTCCAGCGACAATAATGGTAATAATAATAATAATAATAGTAATAATGCGCTTGAATCCGATTTTGGATCGGAGCGAGCTTTGTCCCTCTGGGCCCCGATACTGCTGGGTATGATGGGAAATCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTTGACGAAGATGATGATGATAATAATGATCAGGAGCATGGGGATGGGGAGGGGAACCGTGTGAGTAGTGAGCCGACTGAGCTTGATTCGGTCATTAGGAGGAGGAGGAGAAGTTCGGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGGTGAATCAGAGAATAGTTACGAGGGAGAGAGGAGCAGAGAAAGAGAAAGAGAACGAGAACGAGAAAGAGAGCGGGTTATCCTTATAAATCCTTTCAACCAGACCATTGTAGTTCAAGGAGGTGAGAGTCAAAATCATAACGCTATAGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCCCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTGAGAGTTGAATGCGGGGATAATCGTCTGCAATGTTCGGTGTGTTTGGATGAGTTTGAGATGGATGAAGAAGCCAAGGAGATGCCTTGTAGGCATAAGTTTCATTCAGGGTGTATTCTGCCGTGGCTGGAGCTGCATAGTTCCTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAGTCCAAGCGTGATGCGGATGGTGGGTCGGCTAGGTTTATGGGTAGTGGCAGTGGCAGTGGCAGTGGCAGTGGGGAGATTGATGGGAGAAACGCATCTGGGACTGGGAGAAGGTTTTCGTTTCCATGGCCATTCAATGGCTTGTTTTCTTCGCAAGGAAACTCCTCGGGAGGAAGTGGAAGTGAGTCTCAAAGGGATCACACATCCATTTGA

Coding sequence (CDS)

ATGGACGAGTCAATTGCAACAAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCGTTGACGTGGAAATTAAATGCCCTTTCTGCCGTAGTGGCTTTGTTGAAGAGATGGGTAACGGTATGTCCAGCGACAATAATGGTAATAATAATAATAATAATAGTAATAATGCGCTTGAATCCGATTTTGGATCGGAGCGAGCTTTGTCCCTCTGGGCCCCGATACTGCTGGGTATGATGGGAAATCCCCGTGGGCGTCGTAGATTCAGGCATCTAGAGTTTGACGAAGATGATGATGATAATAATGATCAGGAGCATGGGGATGGGGAGGGGAACCGTGTGAGTAGTGAGCCGACTGAGCTTGATTCGGTCATTAGGAGGAGGAGGAGAAGTTCGGCTACCATTCTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGGTGAATCAGAGAATAGTTACGAGGGAGAGAGGAGCAGAGAAAGAGAAAGAGAACGAGAACGAGAAAGAGAGCGGGTTATCCTTATAAATCCTTTCAACCAGACCATTGTAGTTCAAGGAGGTGAGAGTCAAAATCATAACGCTATAGGGTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAGAATGATCCCAACAGATATGGCACTCCACCAGCCCAGAAGGAGGCAGTTGATGCGCTGCCCACGGTGAGAGTTGAATGCGGGGATAATCGTCTGCAATGTTCGGTGTGTTTGGATGAGTTTGAGATGGATGAAGAAGCCAAGGAGATGCCTTGTAGGCATAAGTTTCATTCAGGGTGTATTCTGCCGTGGCTGGAGCTGCATAGTTCCTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAGTCCAAGCGTGATGCGGATGGTGGGTCGGCTAGGTTTATGGGTAGTGGCAGTGGCAGTGGCAGTGGCAGTGGGGAGATTGATGGGAGAAACGCATCTGGGACTGGGAGAAGGTTTTCGTTTCCATGGCCATTCAATGGCTTGTTTTCTTCGCAAGGAAACTCCTCGGGAGGAAGTGGAAGTGAGTCTCAAAGGGATCACACATCCATTTGA

Protein sequence

MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNALESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADESKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGSESQRDHTSI

Homology

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BLAST of CmoCh20G000050 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.0e-47
Identity = 140/380 (36.84%), Postives = 193/380 (50.79%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           M E+  TRYWCH C Q +   +  EIKCP C  GFVEEM     +D       N      
Sbjct: 1   MAEAAITRYWCHECEQAIEEAMVDEIKCPSCGGGFVEEM-----TDEEIERLTNRQ---- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
                 E   S W PI                 E   +  D++D+++  G          
Sbjct: 61  -----PEPGFSQWNPI-----------------EHPGETMDSDDEDNDLGR--------- 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           E +  IRR RR+S T+ ++L  I   +A                    ++ER+  ILIN 
Sbjct: 121 EFEGFIRRHRRAS-TLRRVLDSIHDDLAD-------------------DQERDSSILINA 180

Query: 181 FNQTIVVQGG----------ESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPA 240
           FNQ + +QG           +  + N  G L +Y +G GL LLLQHLAE+DP+R GTPPA
Sbjct: 181 FNQALALQGSVLDPDEGQGDQGGSTNDDGLLEEYVLGAGLSLLLQHLAESDPSRNGTPPA 240

Query: 241 QKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCP 300
           +KEAV+ALPTV++E     + CSVCLD+ E+  +AK+MPC HKFHS CILPWLELHSSCP
Sbjct: 241 KKEAVEALPTVKIE---EVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSSCP 300

Query: 301 VCRHQLPADESKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSF-PWPFNGLFS 360
           VCR +LP++E+K   +  +   +          G   G  +  + R ++  PW  N LFS
Sbjct: 301 VCRFELPSEETKDLNEPSNIGRVEDSHEEVRADG--PGNVSESSNRPWAIVPW-LNELFS 314

Query: 361 S-QGNSSGGSGSESQRDHTS 369
           + +  ++GG  ++ Q  HTS
Sbjct: 361 TREAQNAGGVSTDQQSPHTS 314

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BLAST of CmoCh20G000050 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 5.7e-38
Identity = 103/248 (41.53%), Postives = 136/248 (54.84%), Query Frame = 0


Query: 134 ATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINPFN------QTI-- 193
           +T+L L+ G  A   +G    S  G   + + R  + +  +    +PF       QT+  
Sbjct: 80  STLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQ-QNPQSDAFDPFTFLQNHLQTLRS 139

Query: 194 -------VVQGGESQNHNAI-GSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDA 253
                  V++   S   N + G+ GDYF GPGL+ L+Q LAENDPNRYGTPPA K A+DA
Sbjct: 140 SGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDA 199

Query: 254 LPTVRVE---CGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRH 313
           LPTV+V          QC+VC+DEFE   + K+MPC+H FH  C+LPWLELH+SCPVCR 
Sbjct: 200 LPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRF 259

Query: 314 QLPADESKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFS--FPWPFNGLFSSQG 361
           +LP D+   +      R  GS  GSG G G ++G+       RFS   PWPF      Q 
Sbjct: 260 ELPTDDPDYE-----NRSQGS-QGSGDGQGSVEGQQTP----RFSIQLPWPFR----RQD 312

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BLAST of CmoCh20G000050 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.6e-35
Identity = 120/375 (32.00%), Postives = 166/375 (44.27%), Query Frame = 0


Query: 9   YWCHMCSQMVNPIV---DVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNALESDF- 68
           ++C+ C++ VN  +     +  CP C  GF+EE  N   +  +G  N N ++      F 
Sbjct: 23  FFCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFL 82

Query: 69  -GSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHG---DGEGNRVSSEP- 128
             S+   SL  P+L     +                D NN    G    G G+  + +P 
Sbjct: 83  TLSDPFASL-LPLLFPSSSSTT-------TSSSASIDPNNPSLSGPTRSGRGDPFAFDPF 142

Query: 129 TELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILIN 188
           T + + +   R S A I                                    E VI  N
Sbjct: 143 TFIQNHLNDLRSSGAQI------------------------------------EFVIQNN 202

Query: 189 PFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALP 248
           P            Q      ++GDYF+GPGL+ L+Q LAENDPNRYGTPPA K A++ALP
Sbjct: 203 P----------SDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALP 262

Query: 249 TVRV---ECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQL 308
            V +          QC+VC+D+FE   EAK+MPC+H +H  C+LPWLELH+SCPVCRH+L
Sbjct: 263 LVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHEL 322

Query: 309 PADESKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGR------RFSFPWPFNGLFSS 366
           P D+   +      R  G+   SG   G+  G+ + G  R      R S PWPF      
Sbjct: 323 PTDDPDYE-----RRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFRISLPWPFQA--RG 336

[Close]

BLAST of CmoCh20G000050 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 5.0e-26
Identity = 71/175 (40.57%), Postives = 97/175 (55.43%), Query Frame = 0


Query: 199 GSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV---ECGDNRLQCSVC 258
           G+ GDYF GPGL+ L + L+     R G PPA + A+DALPT+++       +   C VC
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 259 LDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPA---DESKRDADGGSARF 318
            DEFE+  EAK+MPC H +HS CI+PWL  H+SCPVCR +LP+     S ++    +  +
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 319 MGSGSGSGSGSGEIDGRNASGTGRRFSFPWPF--NGLFSSQGNSSGGSGSESQRD 366
             S S S S S E    N +     FS  WPF  +G  SS   + GG+ +    D
Sbjct: 255 RSSSSSSSSNSRE----NGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNSDTSD 304

[Close]

BLAST of CmoCh20G000050 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 3.3e-25
Identity = 68/167 (40.72%), Postives = 92/167 (55.09%), Query Frame = 0


Query: 202 GDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRV---ECGDNRLQCSVCLDE 261
           GDYF GPGL+ L++ L+    +R G PPA K ++DALPT+++       +   C VC DE
Sbjct: 134 GDYFFGPGLEELIEQLSSGTHHR-GPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDE 193

Query: 262 FEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADESKRDADGGSARFMGSGSG 321
           FE+  EAK+MPC H +HS CI+PWL  H+SCPVCR +LP+  S             S S 
Sbjct: 194 FELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGS-------------SSST 253

Query: 322 SGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGSESQRD 366
             S +   +GR  S     FS  WPF         SS  S ++++RD
Sbjct: 254 QSSQNRSTNGRENSRRRNIFSNLWPF--------RSSSSSSTQNRRD 278

[Close]

BLAST of CmoCh20G000050 vs. ExPASy TrEMBL
Match: A0A6J1FWF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781 PE=4 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.1e-209
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240
           FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT
Sbjct: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240

Query: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE 300
           VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE
Sbjct: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE 300

Query: 301 SKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGS 360
           SKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGS
Sbjct: 301 SKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGS 360

Query: 361 ESQRDHTSI 370
           ESQRDHTSI
Sbjct: 361 ESQRDHTSI 369

[Close]

BLAST of CmoCh20G000050 vs. ExPASy TrEMBL
Match: A0A6J1JDY5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 4.8e-197
Identity = 355/376 (94.41%), Postives = 360/376 (95.74%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG NNNNNSNNAL
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG-NNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP 
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILI 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILI
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180

Query: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL
Sbjct: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPA 300
           PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQLPA
Sbjct: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPA 300

Query: 301 DESKRDADGGSARFM-----GSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGN 360
           DESKRD DGGSARFM     G+ SGSGSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGN
Sbjct: 301 DESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGN 360

Query: 361 SSGGSGSESQRDHTSI 370
           SSGGS SESQRDHT I
Sbjct: 361 SSGGSESESQRDHTPI 375

[Close]

BLAST of CmoCh20G000050 vs. ExPASy TrEMBL
Match: A0A6J1JBZ6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.5e-195
Identity = 351/371 (94.61%), Postives = 355/371 (95.69%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG NNNNNSNNAL
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG-NNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP 
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILI 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILI
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180

Query: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL
Sbjct: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPA 300
           PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQLPA
Sbjct: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPA 300

Query: 301 DESKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGS 360
           DESKRD DGGSARFM      GSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGNSSGGS
Sbjct: 301 DESKRDTDGGSARFM------GSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNSSGGS 360

Query: 361 GSESQRDHTSI 370
            SESQRDHT I
Sbjct: 361 ESESQRDHTPI 364

[Close]

BLAST of CmoCh20G000050 vs. ExPASy TrEMBL
Match: A0A1S3BNR7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.3e-173
Identity = 321/378 (84.92%), Postives = 342/378 (90.48%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN NNNNNN+NN L
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGN VSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNHVSSETT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           ELDSVI RRRRSSATILQLLQGIRAG+AT ESENSYEGERS        RERERVILINP
Sbjct: 121 ELDSVI-RRRRSSATILQLLQGIRAGIATAESENSYEGERS--------RERERVILINP 180

Query: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           FNQTIVVQGGE  +QNHN+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+RLQCSVCLDEFE+DEEAKEMPC+HKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300

Query: 301 ADESKRDADGGS-ARFM-----GSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQ 360
            DESKRD DGGS ARFM     G+G+G+G+GSGEI+ RN+S +GRRFSFPWPFNGLF+SQ
Sbjct: 301 GDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQ 360

Query: 361 GNSSGGSGSESQRDHTSI 370
           GNSS GS S+SQRDHTSI
Sbjct: 361 GNSSAGSASDSQRDHTSI 366

[Close]

BLAST of CmoCh20G000050 vs. ExPASy TrEMBL
Match: A0A5D3DDU3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001160 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.3e-173
Identity = 321/378 (84.92%), Postives = 342/378 (90.48%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN NNNNNN+NN L
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGN VSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNHVSSETT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           ELDSVI RRRRSSATILQLLQGIRAG+AT ESENSYEGERS        RERERVILINP
Sbjct: 121 ELDSVI-RRRRSSATILQLLQGIRAGIATAESENSYEGERS--------RERERVILINP 180

Query: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           FNQTIVVQGGE  +QNHN+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVEC-GDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+RLQCSVCLDEFE+DEEAKEMPC+HKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300

Query: 301 ADESKRDADGGS-ARFM-----GSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQ 360
            DESKRD DGGS ARFM     G+G+G+G+GSGEI+ RN+S +GRRFSFPWPFNGLF+SQ
Sbjct: 301 GDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQ 360

Query: 361 GNSSGGSGSESQRDHTSI 370
           GNSS GS S+SQRDHTSI
Sbjct: 361 GNSSAGSASDSQRDHTSI 366

[Close]

BLAST of CmoCh20G000050 vs. NCBI nr
Match: XP_022944289.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata])

HSP 1 Score: 738.8 bits (1906), Expect = 2.3e-209
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240
           FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT
Sbjct: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240

Query: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE 300
           VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE
Sbjct: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE 300

Query: 301 SKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGS 360
           SKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGS
Sbjct: 301 SKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGS 360

Query: 361 ESQRDHTSI 370
           ESQRDHTSI
Sbjct: 361 ESQRDHTSI 369

[Close]

BLAST of CmoCh20G000050 vs. NCBI nr
Match: KAG7010206.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 720.3 bits (1858), Expect = 8.4e-204
Identity = 364/369 (98.64%), Postives = 365/369 (98.92%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG NNNNNSNNAL
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG-NNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240
           FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT
Sbjct: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240

Query: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE 300
           VRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQLPADE
Sbjct: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADE 300

Query: 301 SKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGS 360
           SKRDADGGSARFM  GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGS S
Sbjct: 301 SKRDADGGSARFM--GSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSES 360

Query: 361 ESQRDHTSI 370
           ESQRDHTSI
Sbjct: 361 ESQRDHTSI 366

[Close]

BLAST of CmoCh20G000050 vs. NCBI nr
Match: XP_023512553.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 702.6 bits (1812), Expect = 1.8e-198
Identity = 357/373 (95.71%), Postives = 360/373 (96.51%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDESI TRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNG+SS+NNG NNNNNSNNAL
Sbjct: 1   MDESIPTRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGVSSENNG-NNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS----RERERERERERERVI 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS    RERERERERERERVI
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERERVI 180

Query: 181 LINPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVD 240
           LINPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVD
Sbjct: 181 LINPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVD 240

Query: 241 ALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQL 300
           ALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQL
Sbjct: 241 ALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQL 300

Query: 301 PADESKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSG 360
           PADESKRDADGGSARFM      GSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSG
Sbjct: 301 PADESKRDADGGSARFM------GSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSG 360

Query: 361 GSGSESQRDHTSI 370
           GS SESQRDHTSI
Sbjct: 361 GSESESQRDHTSI 366

[Close]

BLAST of CmoCh20G000050 vs. NCBI nr
Match: XP_022985674.1 (E3 ubiquitin-protein ligase SIRP1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 696.8 bits (1797), Expect = 9.9e-197
Identity = 355/376 (94.41%), Postives = 360/376 (95.74%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG NNNNNSNNAL
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG-NNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP 
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILI 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILI
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180

Query: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL
Sbjct: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPA 300
           PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQLPA
Sbjct: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPA 300

Query: 301 DESKRDADGGSARFM-----GSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGN 360
           DESKRD DGGSARFM     G+ SGSGSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGN
Sbjct: 301 DESKRDTDGGSARFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGN 360

Query: 361 SSGGSGSESQRDHTSI 370
           SSGGS SESQRDHT I
Sbjct: 361 SSGGSESESQRDHTPI 375

[Close]

BLAST of CmoCh20G000050 vs. NCBI nr
Match: XP_022985675.1 (E3 ubiquitin-protein ligase SIRP1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 691.8 bits (1784), Expect = 3.2e-195
Identity = 351/371 (94.61%), Postives = 355/371 (95.69%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           MDESIATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG NNNNNSNNAL
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNG-NNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDD+NN+QEHGDGEGNR SSEP 
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS--RERERERERERERVILI 180
           ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERS  RERERERERERERVILI
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180

Query: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240
           NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL
Sbjct: 181 NPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPA 300
           PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPC+HKFHSGCILPWLELHSSCPVCRHQLPA
Sbjct: 241 PTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPA 300

Query: 301 DESKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGS 360
           DESKRD DGGSARFM      GSGSGEIDGR ASG GRRFSFPWPFNGLFSSQGNSSGGS
Sbjct: 301 DESKRDTDGGSARFM------GSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNSSGGS 360

Query: 361 GSESQRDHTSI 370
            SESQRDHT I
Sbjct: 361 ESESQRDHTPI 364

[Close]

BLAST of CmoCh20G000050 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.2e-104
Identity = 216/397 (54.41%), Postives = 272/397 (68.51%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEM-GNGMSSDNNGNNNNNNSNNA 60
           M+E++A RYWCHMCSQMVNP+++ EIKCPFC+SGF+EEM GNG      G  +  +S   
Sbjct: 1   MEETMAARYWCHMCSQMVNPVMESEIKCPFCQSGFIEEMSGNGGGGGGRGIRDVQDS--- 60

Query: 61  LESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQ--EHGDGEGNRVSS 120
            E+DFG++RALSLWAPILLGMM +PR RRRFR  EF E++DDN D    + DG GN  ++
Sbjct: 61  -ETDFGTDRALSLWAPILLGMMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNN 120

Query: 121 E-------------------PTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEG 180
                                 E +S++RRRRRSS  ILQLLQGIRAG+A     + YE 
Sbjct: 121 SNNNVYRHHRARRHGGEIDLDREFESILRRRRRSSGNILQLLQGIRAGIA-----SEYE- 180

Query: 181 ERSRERERERERERERVILINPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLA 240
             S +   +  RER+RVI+INP+NQ++VV   ++QNH ++ SLGDYF+GPGLDLLLQHLA
Sbjct: 181 --SSDNNWDNSRERDRVIMINPYNQSLVVPSDQNQNHPSLTSLGDYFIGPGLDLLLQHLA 240

Query: 241 ENDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGC 300
           ENDPNR GTPPA+KEAV+ALPTV++      LQCSVCLD+FE   EAKEMPC+HKFH  C
Sbjct: 241 ENDPNRQGTPPARKEAVEALPTVKIM---EPLQCSVCLDDFEKGTEAKEMPCKHKFHVRC 300

Query: 301 ILPWLELHSSCPVCRHQLPA-----DESKRDAD----GGSARFMGSGS---GSGSGSGEI 360
           I+PWLELHSSCPVCR +LP+     DE+K D++      + R   +G+     G+     
Sbjct: 301 IVPWLELHSSCPVCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRGR 360

Query: 361 DGRNASGTGRRFSFPWPFNGLFSSQGNSSGG-SGSES 363
           +    SG GRRFSFPWPF+GLFSS  +SS   SGS+S
Sbjct: 361 EDEVRSGNGRRFSFPWPFSGLFSSSSSSSSSTSGSQS 382

[Close]

BLAST of CmoCh20G000050 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.9e-85
Identity = 186/386 (48.19%), Postives = 242/386 (62.69%), Query Frame = 0


Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNALESDF 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEEM   +   N G++++       E DF
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREI---NGGSSSSLREVQDPEIDF 60

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDG-------------- 124
           G++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +  DG              
Sbjct: 61  GTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRH 120

Query: 125 ----EGNRVSSEPTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERER 184
               +G  +  +  E +S++RRRRRSSATILQLLQGIRAG+A+ E E+S           
Sbjct: 121 RHRQQGREIDLD-REFESILRRRRRSSATILQLLQGIRAGIAS-EYESS----------- 180

Query: 185 ERERERERVILINPFNQTIVVQGGESQNHN----AIGSLGDYFVGPGLDLLLQHLAENDP 244
                 +R +L    NQ+ VVQG  S N N    ++ ++GDYFVG  LD LL+HLA+ND 
Sbjct: 181 ------DRDLL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDS 240

Query: 245 NRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPW 304
            R+G+ PA+KE VD LPTV++      LQCS+CLD+F+   EAKEMPC+HKFH  CI+PW
Sbjct: 241 IRHGSLPARKEVVDNLPTVKI---SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPW 300

Query: 305 LELHSSCPVCRHQLPADESKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPW 364
           LELHSSCPVCR++LP D+  +  +    R         + + E D RN++ + RRFSFPW
Sbjct: 301 LELHSSCPVCRYELPPDDETK-VNPVRPRTRTLEINVSNENVEDDARNSNVSERRFSFPW 356

Query: 365 PFNGLFSSQGNSSGGSGSESQRDHTS 369
           PF+GLFSS  +SS  +   S     S
Sbjct: 361 PFSGLFSSSSSSSASASGSSHLGENS 356

[Close]

BLAST of CmoCh20G000050 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.9e-85
Identity = 186/386 (48.19%), Postives = 242/386 (62.69%), Query Frame = 0


Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNALESDF 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEEM   +   N G++++       E DF
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREI---NGGSSSSLREVQDPEIDF 60

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDG-------------- 124
           G++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +  DG              
Sbjct: 61  GTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRH 120

Query: 125 ----EGNRVSSEPTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERER 184
               +G  +  +  E +S++RRRRRSSATILQLLQGIRAG+A+ E E+S           
Sbjct: 121 RHRQQGREIDLD-REFESILRRRRRSSATILQLLQGIRAGIAS-EYESS----------- 180

Query: 185 ERERERERVILINPFNQTIVVQGGESQNHN----AIGSLGDYFVGPGLDLLLQHLAENDP 244
                 +R +L    NQ+ VVQG  S N N    ++ ++GDYFVG  LD LL+HLA+ND 
Sbjct: 181 ------DRDLL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDS 240

Query: 245 NRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPW 304
            R+G+ PA+KE VD LPTV++      LQCS+CLD+F+   EAKEMPC+HKFH  CI+PW
Sbjct: 241 IRHGSLPARKEVVDNLPTVKI---SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPW 300

Query: 305 LELHSSCPVCRHQLPADESKRDADGGSARFMGSGSGSGSGSGEIDGRNASGTGRRFSFPW 364
           LELHSSCPVCR++LP D+  +  +    R         + + E D RN++ + RRFSFPW
Sbjct: 301 LELHSSCPVCRYELPPDDETK-VNPVRPRTRTLEINVSNENVEDDARNSNVSERRFSFPW 356

Query: 365 PFNGLFSSQGNSSGGSGSESQRDHTS 369
           PF+GLFSS  +SS  +   S     S
Sbjct: 361 PFSGLFSSSSSSSASASGSSHLGENS 356

[Close]

BLAST of CmoCh20G000050 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 310.5 bits (794), Expect = 1.9e-84
Identity = 183/379 (48.28%), Postives = 238/379 (62.80%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60
           M+E++ TRYWCHMCSQ VNP+++ EIKCPFC+SGFVEEM +    D++   +   +N   
Sbjct: 1   MEEAVVTRYWCHMCSQTVNPVMEAEIKCPFCQSGFVEEMEDDDDHDSSDPADVRANN--- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120
                     SLWAPIL+ +M +P  RRR + +E  ED+ +    E+ + +G   +    
Sbjct: 61  ----------SLWAPILMELMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE--NDLDW 120

Query: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180
           +L  ++RRRRR SA +LQLLQGIRAG++  ESE++  G  +            RVILIN 
Sbjct: 121 QLQEILRRRRRHSAAVLQLLQGIRAGLSV-ESESTGNGGNN----------PGRVILINT 180

Query: 181 FNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPT 240
            NQTI VQ     +    GSLGDYF+GPG ++LLQ LAENDPNRYGTPPA+KEAV+AL T
Sbjct: 181 SNQTITVQNSADMDSVPAGSLGDYFIGPGFEMLLQRLAENDPNRYGTPPAKKEAVEALAT 240

Query: 241 VRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADE 300
           V++E     LQCSVCLD+FE+  EAK MPC HKFHS C+LPWLELHSSCPVCR+QLPADE
Sbjct: 241 VKIE---ETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADE 300

Query: 301 SKRDADGGSARFMGSGSGSGS---GSGEIDG----------------RNASGTGRRFSFP 360
           +K D+   ++   GS S S +   G+   DG                 N +  G  FS P
Sbjct: 301 AKTDSVTTTSDNNGSSSASATTSHGAENSDGNRRQEEEEEEEEEEEEENENNDGSGFSIP 350

[Close]

BLAST of CmoCh20G000050 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 241.5 bits (615), Expect = 1.1e-63
Identity = 155/347 (44.67%), Postives = 198/347 (57.06%), Query Frame = 0


Query: 1   MDESIATRYWCHMCSQMVNPIVDVE-IKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNA 60
           M+++  T YWCHMCS+ V P++  E IKC FC+SGFVEEM      DNN ++        
Sbjct: 1   MEDASETSYWCHMCSRSVIPLIQDEIIKCNFCQSGFVEEM------DNNDDH-------- 60

Query: 61  LESDFGSERALSLWAPILLGMMGNPRGRRRFRHLE----FDEDDDDNNDQEHGDGEGNRV 120
                  + A SLW PIL+ MM N   +      +     +++D+D +D + GD   +  
Sbjct: 61  -------QAADSLWTPILMEMMNNNHDQHSTNQEDSESILEDEDEDEDDGDDGDQNNDGE 120

Query: 121 SSEPTELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERV 180
                +L+ + R R R S  I+ LLQGIRAG+   ESEN+           +     E V
Sbjct: 121 IDITHQLEEIRRIRTRHSTAIVNLLQGIRAGLLI-ESENN----------EDNPDNSELV 180

Query: 181 ILINPFNQTI-VVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAEND-PNRYGTPPAQKE 240
           +LIN FNQ I V Q          GSLGDYF+GPG + LLQ LAEND  NRYGTPPA KE
Sbjct: 181 VLINSFNQRIRVHQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKE 240

Query: 241 AVDALPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCR 300
           AV+AL  V++E  D+ LQCSVCLD+FE+  EAKEMPC+HKFHS C+LPWLELHSSCPVCR
Sbjct: 241 AVEALAMVKIE--DSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCR 300

Query: 301 HQLPA---DESKRDA-----DGGSARFMGSGSGSGSGSGEIDGRNAS 333
           + LP    DE K DA     D  +     +   S   S +    N+S
Sbjct: 301 YLLPTGDDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNSS 313

The following BLAST results are available for this feature:

BLAST of CmoCh20G000050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6AVN2	1.0e-47	36.84	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8LPN7	5.7e-38	41.53	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	1.6e-35	32.00	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22197	5.0e-26	40.57	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	3.3e-25	40.72	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]

BLAST of CmoCh20G000050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FWF9	1.1e-209	100.00	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781...	[more]
A0A6J1JDY5	4.8e-197	94.41	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 P...	[more]
A0A6J1JBZ6	1.5e-195	94.61	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 P...	[more]
A0A1S3BNR7	6.3e-173	84.92	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 ...	[more]
A0A5D3DDU3	6.3e-173	84.92	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of CmoCh20G000050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022944289.1	2.3e-209	100.00	E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata]	[more]
KAG7010206.1	8.4e-204	98.64	E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023512553.1	1.8e-198	95.71	E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022985674.1	9.9e-197	94.41	E3 ubiquitin-protein ligase SIRP1-like isoform X1 [Cucurbita maxima]	[more]
XP_022985675.1	3.2e-195	94.61	E3 ubiquitin-protein ligase SIRP1-like isoform X2 [Cucurbita maxima]	[more]

BLAST of CmoCh20G000050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56340.1	1.2e-104	54.41	RING/U-box superfamily protein	[more]
AT4G26400.2	2.9e-85	48.19	RING/U-box superfamily protein	[more]
AT4G26400.1	2.9e-85	48.19	RING/U-box superfamily protein	[more]
AT1G55530.1	1.9e-84	48.28	RING/U-box superfamily protein	[more]
AT3G13430.1	1.1e-63	44.67	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 252..292 e-value: 8.4E-8 score: 41.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 251..293 e-value: 1.1E-12 score: 48.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 252..293 score: 12.415513
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 216..299 e-value: 9.2E-21 score: 75.3
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 7..39 e-value: 3.4E-12 score: 46.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 299..369
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 148..170
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 107..128
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 314..328
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 155..170
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 344..369
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 94..128
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 3..311
None	No IPR available	PANTHER	PTHR15710:SF22	RING/U-BOX SUPERFAMILY PROTEIN	coord: 3..311
None	No IPR available	CDD	cd16669	RING-H2_RNF181	coord: 251..296 e-value: 5.6661E-23 score: 88.5817
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 247..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh20G000050.1	CmoCh20G000050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016020	membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity