CmoCh20G000010 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G000010
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionL-ascorbate oxidase homolog
LocationCmo_Chr20: 88394 .. 90150 (+)
RNA-Seq ExpressionCmoCh20G000010
SyntenyCmoCh20G000010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAGGGTGATGTTTAGCGCGCTGCTGCAGCTTCTATGCCTCTCCAGTGTAGCAGTGTGGACGGTCCGGGGTGAAGATCCTTATTTCTTCTTCGTTTGGAACGTCTCCTACGGCACCATCTCTCCCTTGGGCGTTCCCCAGCAAGCCATTCTCATCAATGGCCAATTCCCCGGACCTAATATCAACTCCACCTCCAACAATAACGTTATCGTTAACGTCTTCAACAACCTGGACGAGCCCTTCCTTCTTCATTGGAACGGTATTCAACACAGAAAGAACTCGTGGCAAGATGGACAACTTGGAACCAATTGCCCAATCCCCCCCGGGACCAACTACACTTACCGTTTCCAAGTTAAGGACCAAATTGGTAGTTTCTTCTACTACCCATCAATTGGCCTGCACAGGGCAGCAGGAGCATTCGGCGGCCTCCGTATTAACAGTCGCTTACTCATTCCTGTCCCTTACGCTGACCCTGAAGATGACTACACTGTCCTTATTGGTGACTGGTATACCAAGAGCCACACCACTCTTAGGAAGATCTTGGATAGCGGTGGCCGCTCCATTGCTAGGCCCAACGGGGTCCTCATCAATGGCAAGATTGCGAAGGGGGACGGAACCGATGAGCCACTGTTCACCATGAAGCCTGGAAAGACCTACAAGTATCGAATTTGCAACGTGGGACTCAAGGCATCTCTCAACCTTAGATTCCAAGGCCACACCATGAAATTAGTGGAGATGGAAGGTTCTCATACCGTTCAAAACGACTACCAATCACTGGACGTCCACGTGGGGCAATGCTTTTCGGTGCTAGTCACGGCCGACCAAGAGCCCAAGGACTATTACCTAGTTGCATCCACTAGATTCATGAAGAATGTTCTGGTTGGTAAGGGCATTATTCGATACACCAATGGCAAGGGTCCCGCCTCTCCTGAAATTCCAGAGGCTCCCACTGGTTGGATTTGGTCTCTCAATCAGTTTCGTTCCTTCCGCTGGAACCTTACTGCTAGTGCTGCAAGGCCTAATCCCCAAGGCTCCTACAGGTATGGTTCCATCAACATTACGCGTACCATCAAGCTATTCAATTCAGTTAGTAAGGTGGATGGTAAGTTGCGTTATGCCATTAACGGCGTCTCTCACATTGATCCTGAAACTCCATTGAAACATGCGGAGTACTTCGGAGTTGGTGATAAGGTCTTCAAATACAATATCATTTCGGATGAGGGACCACCTGAAGGCTCGACCTCAATCACTCTTGCTCCCAATGTTGTCAATGTAACCTTCCGAAACTTCGTAGAGATCATTTTTGAGAATCGTGAGAAGAGTATACAATCATGGCATTTGAATGGCTACTCTTTCTTTGCGGTTGCGTAAGTGGTCACTTCAAATGTTTAATACAAATTTCGTAATCTCGGCTTCAACATGCCTAATAAATCCCTTATTCAATATTGTACAGCATCGAGCCTGGGAGATGGTCTCCAGACAAACGATCCAACTACAATCTTCTAGATGCAGTGAGCAGACATACAATTCAAGTGTACCCTAATTCATGGGCAGCCATTTTCCTTACATTCGATAATGCTGGAATGTGGAATTTGAGGTCTGAGTTGACAGAAGACCGTTACTTAGGACAGCAACTTTACATCAGTGTTCTTTCACCAGCTCTCTCCCTTAGAGATGAGTACAACATCCCAGATAACGTTTTGCTTTGTGGTTTGGTGAAGGGCATGCCATTGCCCAAGCCATATTCTATTTGA

mRNA sequence

ATGGGTAGGGTGATGTTTAGCGCGCTGCTGCAGCTTCTATGCCTCTCCAGTGTAGCAGTGTGGACGGTCCGGGGTGAAGATCCTTATTTCTTCTTCGTTTGGAACGTCTCCTACGGCACCATCTCTCCCTTGGGCGTTCCCCAGCAAGCCATTCTCATCAATGGCCAATTCCCCGGACCTAATATCAACTCCACCTCCAACAATAACGTTATCGTTAACGTCTTCAACAACCTGGACGAGCCCTTCCTTCTTCATTGGAACGGTATTCAACACAGAAAGAACTCGTGGCAAGATGGACAACTTGGAACCAATTGCCCAATCCCCCCCGGGACCAACTACACTTACCGTTTCCAAGTTAAGGACCAAATTGGTAGTTTCTTCTACTACCCATCAATTGGCCTGCACAGGGCAGCAGGAGCATTCGGCGGCCTCCGTATTAACAGTCGCTTACTCATTCCTGTCCCTTACGCTGACCCTGAAGATGACTACACTGTCCTTATTGGTGACTGGTATACCAAGAGCCACACCACTCTTAGGAAGATCTTGGATAGCGGTGGCCGCTCCATTGCTAGGCCCAACGGGGTCCTCATCAATGGCAAGATTGCGAAGGGGGACGGAACCGATGAGCCACTGTTCACCATGAAGCCTGGAAAGACCTACAAGTATCGAATTTGCAACGTGGGACTCAAGGCATCTCTCAACCTTAGATTCCAAGGCCACACCATGAAATTAGTGGAGATGGAAGGTTCTCATACCGTTCAAAACGACTACCAATCACTGGACGTCCACGTGGGGCAATGCTTTTCGGTGCTAGTCACGGCCGACCAAGAGCCCAAGGACTATTACCTAGTTGCATCCACTAGATTCATGAAGAATGTTCTGGTTGGTAAGGGCATTATTCGATACACCAATGGCAAGGGTCCCGCCTCTCCTGAAATTCCAGAGGCTCCCACTGGTTGGATTTGGTCTCTCAATCAGTTTCGTTCCTTCCGCTGGAACCTTACTGCTAGTGCTGCAAGGCCTAATCCCCAAGGCTCCTACAGGTATGGTTCCATCAACATTACGCGTACCATCAAGCTATTCAATTCAGTTAGTAAGGTGGATGGTAAGTTGCGTTATGCCATTAACGGCGTCTCTCACATTGATCCTGAAACTCCATTGAAACATGCGGAGTACTTCGGAGTTGGTGATAAGGTCTTCAAATACAATATCATTTCGGATGAGGGACCACCTGAAGGCTCGACCTCAATCACTCTTGCTCCCAATGTTGTCAATGTAACCTTCCGAAACTTCGTAGAGATCATTTTTGAGAATCGTGAGAAGAGTATACAATCATGGCATTTGAATGGCTACTCTTTCTTTGCGGTTGCCATCGAGCCTGGGAGATGGTCTCCAGACAAACGATCCAACTACAATCTTCTAGATGCAGTGAGCAGACATACAATTCAAGTGTACCCTAATTCATGGGCAGCCATTTTCCTTACATTCGATAATGCTGGAATGTGGAATTTGAGGTCTGAGTTGACAGAAGACCGTTACTTAGGACAGCAACTTTACATCAGTGTTCTTTCACCAGCTCTCTCCCTTAGAGATGAGTACAACATCCCAGATAACGTTTTGCTTTGTGGTTTGGTGAAGGGCATGCCATTGCCCAAGCCATATTCTATTTGA

Coding sequence (CDS)

ATGGGTAGGGTGATGTTTAGCGCGCTGCTGCAGCTTCTATGCCTCTCCAGTGTAGCAGTGTGGACGGTCCGGGGTGAAGATCCTTATTTCTTCTTCGTTTGGAACGTCTCCTACGGCACCATCTCTCCCTTGGGCGTTCCCCAGCAAGCCATTCTCATCAATGGCCAATTCCCCGGACCTAATATCAACTCCACCTCCAACAATAACGTTATCGTTAACGTCTTCAACAACCTGGACGAGCCCTTCCTTCTTCATTGGAACGGTATTCAACACAGAAAGAACTCGTGGCAAGATGGACAACTTGGAACCAATTGCCCAATCCCCCCCGGGACCAACTACACTTACCGTTTCCAAGTTAAGGACCAAATTGGTAGTTTCTTCTACTACCCATCAATTGGCCTGCACAGGGCAGCAGGAGCATTCGGCGGCCTCCGTATTAACAGTCGCTTACTCATTCCTGTCCCTTACGCTGACCCTGAAGATGACTACACTGTCCTTATTGGTGACTGGTATACCAAGAGCCACACCACTCTTAGGAAGATCTTGGATAGCGGTGGCCGCTCCATTGCTAGGCCCAACGGGGTCCTCATCAATGGCAAGATTGCGAAGGGGGACGGAACCGATGAGCCACTGTTCACCATGAAGCCTGGAAAGACCTACAAGTATCGAATTTGCAACGTGGGACTCAAGGCATCTCTCAACCTTAGATTCCAAGGCCACACCATGAAATTAGTGGAGATGGAAGGTTCTCATACCGTTCAAAACGACTACCAATCACTGGACGTCCACGTGGGGCAATGCTTTTCGGTGCTAGTCACGGCCGACCAAGAGCCCAAGGACTATTACCTAGTTGCATCCACTAGATTCATGAAGAATGTTCTGGTTGGTAAGGGCATTATTCGATACACCAATGGCAAGGGTCCCGCCTCTCCTGAAATTCCAGAGGCTCCCACTGGTTGGATTTGGTCTCTCAATCAGTTTCGTTCCTTCCGCTGGAACCTTACTGCTAGTGCTGCAAGGCCTAATCCCCAAGGCTCCTACAGGTATGGTTCCATCAACATTACGCGTACCATCAAGCTATTCAATTCAGTTAGTAAGGTGGATGGTAAGTTGCGTTATGCCATTAACGGCGTCTCTCACATTGATCCTGAAACTCCATTGAAACATGCGGAGTACTTCGGAGTTGGTGATAAGGTCTTCAAATACAATATCATTTCGGATGAGGGACCACCTGAAGGCTCGACCTCAATCACTCTTGCTCCCAATGTTGTCAATGTAACCTTCCGAAACTTCGTAGAGATCATTTTTGAGAATCGTGAGAAGAGTATACAATCATGGCATTTGAATGGCTACTCTTTCTTTGCGGTTGCCATCGAGCCTGGGAGATGGTCTCCAGACAAACGATCCAACTACAATCTTCTAGATGCAGTGAGCAGACATACAATTCAAGTGTACCCTAATTCATGGGCAGCCATTTTCCTTACATTCGATAATGCTGGAATGTGGAATTTGAGGTCTGAGTTGACAGAAGACCGTTACTTAGGACAGCAACTTTACATCAGTGTTCTTTCACCAGCTCTCTCCCTTAGAGATGAGTACAACATCCCAGATAACGTTTTGCTTTGTGGTTTGGTGAAGGGCATGCCATTGCCCAAGCCATATTCTATTTGA

Protein sequence

MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHTMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGIIRYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKLFNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLAPNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRHTIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVLLCGLVKGMPLPKPYSI
Homology
BLAST of CmoCh20G000010 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 5.6e-255
Identity = 403/548 (73.54%), Postives = 458/548 (83.58%), Query Frame = 0

Query: 9   LLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNINSTSNN 68
           L   L L++ A   V  EDPYF  VWNV+YGT SPLGVPQQ ILINGQFPGPNINSTSNN
Sbjct: 7   LAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNN 66

Query: 69  NVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQIGSFFY 128
           NVI+NVFNNLDEPFLL WNGIQHRKN WQDG  GT CPI PGTNYTY FQ KDQIGS+FY
Sbjct: 67  NVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFY 126

Query: 129 YPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKILDSGGRS 188
           YP+ G+HRAAG +GGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHT L+K LD GGR+
Sbjct: 127 YPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLD-GGRT 186

Query: 189 IARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHTMKLVEME 248
           I RP+G++INGK  KGDG+D PLFT+KPGKTY+ RICNVG+K S+N R Q H MKLVEME
Sbjct: 187 IGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEME 246

Query: 249 GSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGIIRYTNGKGP 308
           GSH +QNDY SLDVHVGQCF  +VTA+QEPKDYY+VAS+RF+K V+   G++RY  GKGP
Sbjct: 247 GSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGP 306

Query: 309 ASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKLFNSVSKVD 368
           AS ++P  P GW WSLNQFRSFRWNLTASAARPNPQGSY YG INITRTIKL N+  KVD
Sbjct: 307 ASSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVD 366

Query: 369 GKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLAPNVVNVTF 428
           GKLR+A+NGVSH +PETPLK AEYFG+ DKVFKY+ I+D+  PE   +I + PNV+N+T 
Sbjct: 367 GKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITH 426

Query: 429 RNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRHTIQVYPNS 488
           R FVE++FEN EKS+QSWHL+GYSFF+VA+EPG W+P+KR NYNLLDAVSRHT+QVYP  
Sbjct: 427 RTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKC 486

Query: 489 WAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVLLCGLVKGM 548
           WAAI LTFDN GMWN+RSE TE RYLGQQLY SVLSP  SLRDEYN+P+  L CGLVK  
Sbjct: 487 WAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNT 546

Query: 549 PLP-KPYS 556
           P P  PY+
Sbjct: 547 PKPVNPYA 553

BLAST of CmoCh20G000010 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 3.3e-239
Identity = 386/557 (69.30%), Postives = 455/557 (81.69%), Query Frame = 0

Query: 2   GRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPN 61
           G+V F AL  LLCLS   +     EDPY +F WNV+YGTI+PLGVPQQ ILINGQFPGP 
Sbjct: 4   GKVTFVAL--LLCLSVGVI----AEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPR 63

Query: 62  INSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKD 121
           IN TSNNN++VNVFNNLDEPFL  WNG+QHRKNSWQDG  GT CPI PG N+TYRFQVKD
Sbjct: 64  INCTSNNNIVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKD 123

Query: 122 QIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKI 181
           QIGS+ Y+P+  LHRAAG +G L ++SR LIPVP+ +P D+Y V +GDWY K H TL+KI
Sbjct: 124 QIGSYSYFPTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKI 183

Query: 182 LDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHT 241
           LD GGR+I RP+G++INGK AK     EPLFTM+ GKTY+YR CN+G+++S+N+RFQGH 
Sbjct: 184 LD-GGRTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHP 243

Query: 242 MKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGIIR 301
           MKLVE+EGSHTVQN Y SLD+HVGQC SVLVTADQEPKDYYLV S+RF+K  L    IIR
Sbjct: 244 MKLVELEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIR 303

Query: 302 YTNGKGPASPEIPEAP----TGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRT 361
           Y NGKGPASPE+P  P     G  WS+NQFRSFRWNLTASAARPNPQGSY YG INITRT
Sbjct: 304 YANGKGPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRT 363

Query: 362 IKLFNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSI 421
           IK+FNS+S+V GKLRY +NG+SH + ETPLK  EYFG  +K FKY++++DE P + S  +
Sbjct: 364 IKIFNSMSQVGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPS-KL 423

Query: 422 TLAPNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAV 481
           T+A NV N T+RNFVEIIFEN EK+I+++HL+GYSFFAVA+EPGRWSP+KR NYNL+D +
Sbjct: 424 TIATNVKNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGL 483

Query: 482 SRHTIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPD 541
           SR+ IQVYPNSWAAI LTFDNAGMWNLRSE+ E  YLG+QLY SVLSP+ SLRDEYNIPD
Sbjct: 484 SRNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPD 543

Query: 542 NVLLCGLVKGMPLPKPY 555
           N  LCG+VKG+ +P PY
Sbjct: 544 NHPLCGIVKGLSMPAPY 552

BLAST of CmoCh20G000010 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.4e-157
Identity = 269/545 (49.36%), Postives = 359/545 (65.87%), Query Frame = 0

Query: 27  DPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNINSTSNNNVIVNVFNNLDEPFLLHW 86
           DPY F+ + VSY T SPLGVPQQ I ING+FPGP IN T+N N++VNV N LDE  LLHW
Sbjct: 22  DPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENLVVNVRNKLDEGLLLHW 81

Query: 87  NGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQIGSFFYYPSIGLHRAAGAFGGLRI 146
           NGIQ R+ SWQDG LGTNCPIPP  N+TY FQVKDQIGSFFY+PS+   RA+G FG   +
Sbjct: 82  NGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFPSLHFQRASGGFGSFVV 141

Query: 147 NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKILDSGGRSIARPNGVLINGK------ 206
           N R +IPVP++ P+ D TV IGDWY ++HT LRK LD  G+ +  P+GVLINGK      
Sbjct: 142 NPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDD-GKDLGMPDGVLINGKGPYRYN 201

Query: 207 -IAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHTMKLVEMEGSHTVQNDYQS 266
                DG D    T+ PGKTY+ R+ NVG+  SLN R QGH + L E EGS+TVQ +Y S
Sbjct: 202 DTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLAESEGSYTVQQNYTS 261

Query: 267 LDVHVGQCFSVLVTADQE-PKDYYLVASTRFMKNVL----VGKGIIRYTNGKGPASPEIP 326
           LD+HVGQ +S LVT DQ    DYY+VAS R +   +     G GI++YTN KG A  ++P
Sbjct: 262 LDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGILKYTNSKGKAKGQLP 321

Query: 327 EAPTGWI---WSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKLFNSVS-KVDGK 386
             P       +S+NQ RS RWN++AS ARPNPQGS++YGSIN+T    L N     + GK
Sbjct: 322 PGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTDVYVLRNMPPVTISGK 381

Query: 387 LRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLAPNVVNVTFRN 446
            R  +NG+S  +P TP++ A+   V D    Y +   + P  G   +  A +++N T+R 
Sbjct: 382 RRTTLNGISFKNPSTPIRLADKLKVKD---VYKLDFPKRPLTGPAKV--ATSIINGTYRG 441

Query: 447 FVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRHTIQVYPNSWA 506
           F+E++ +N +  +QS+H++GY+FF V ++ G W+ + R  YN  D ++R TIQVYP +W+
Sbjct: 442 FMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYPGAWS 501

Query: 507 AIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVLLCGLVKGMPL 556
           AI ++ DN G WNLR+E  +  YLGQ+ Y+ V++P  + + E+  PDNVL CG +  +  
Sbjct: 502 AILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCGALSKLQK 560

BLAST of CmoCh20G000010 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.9e-150
Identity = 261/546 (47.80%), Postives = 369/546 (67.58%), Query Frame = 0

Query: 27  DPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNINSTSNNNVIVNVFNNLDEPFLLHW 86
           DPY  + + +SY T SPLGVPQQ I +NG+FPGP IN+T+N NV VNV N+LDEP LL W
Sbjct: 25  DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLLTW 84

Query: 87  NGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQIGSFFYYPSIGLHRAAGAFGGLRI 146
            G+Q R+NSWQDG LGTNCPIPP  N+TY FQ+KDQIGS+FY PS+   RA+G FG L I
Sbjct: 85  PGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGALII 144

Query: 147 NSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKILDSGGRSIARPNGVLINGK------ 206
           N+R L+P+P+ +P+ +   +IGDWYT++HT LR+ILDS G+ +  P+GVLINGK      
Sbjct: 145 NNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDS-GKELGMPDGVLINGKGPFKYN 204

Query: 207 IAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHTMKLVEMEGSHTVQNDYQSL 266
            +  DG +     + PGKTY+ R+ NVG+  SLN R Q H + L+E EG +T Q ++   
Sbjct: 205 SSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTDF 264

Query: 267 DVHVGQCFSVLVTADQE-PKDYYLVASTRFMKNV----LVGKGIIRYTNGKGPASPEIPE 326
           DVHVGQ +S LVT DQ    DYY+VAS RF+       + G GI+ Y+N KGPAS  +P 
Sbjct: 265 DVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLPV 324

Query: 327 APT--GWIWS-LNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKLFN-SVSKVDGKL 386
           + T     WS +NQ R+ + N +AS ARPNPQGS+ YG INITRT  L +   +K++GKL
Sbjct: 325 SATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGKL 384

Query: 387 RYAINGVSHIDPETPLKHAEYFGV-GDKVFKY-NIISDEGPPEGSTSITLAPNVVNVTFR 446
           R  +NG+S ++P TP++ A+   V GD +  + +   DE  P  S+SI      +N T++
Sbjct: 385 RATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPRLSSSI------INATYK 444

Query: 447 NFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRHTIQVYPNSW 506
            F+++IF+N +  IQS+H++GY+F+ VA++ G WS D+ S+YN  DAV+R T++VYP +W
Sbjct: 445 GFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAW 504

Query: 507 AAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVLLCGLVKGMP 556
            A+ ++ DN G+WN+R E  +  YLGQ+ Y+ +++P  +   E + P+NV+ CG ++ M 
Sbjct: 505 TAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAMQ 563

BLAST of CmoCh20G000010 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 261/567 (46.03%), Postives = 370/567 (65.26%), Query Frame = 0

Query: 4   VMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNIN 63
           ++ S LL    LS+V+       DP+  + + VSY T SPLGVPQQ I +NGQFPGP +N
Sbjct: 7   LLASFLLCFALLSAVSF----AADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLN 66

Query: 64  STSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQI 123
           +T+N NV+VNVFN+LDEP LL W GIQ R+NSWQDG LGTNCPIPP  N+TY+FQVKDQI
Sbjct: 67  ATTNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQI 126

Query: 124 GSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKILD 183
           GSFFY PS+   RA+G FG + IN+R +IP+P+  P+ +   +IGDWYT+ H  LR+ LD
Sbjct: 127 GSFFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALD 186

Query: 184 SGGRSIARPNGVLINGK------IAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRF 243
           S G+ +  P+GVLINGK       +  DG D   F ++PGKTY+ R+ NVG+  SLN R 
Sbjct: 187 S-GKELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRI 246

Query: 244 QGHTMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQE-PKDYYLVASTRFMKNV--- 303
           Q H++ LVE EG +T Q ++   DVHVGQ +S LVT DQ+   DYY+VAS RF+      
Sbjct: 247 QNHSLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQ 306

Query: 304 -LVGKGIIRYTNGKGPASP--EIPEAPTGWIWS-LNQFRSFRWNLTASAARPNPQGSYRY 363
            + G  I+ Y+N KGP S    +P+      WS ++Q ++ R N +AS ARPNPQGS+ Y
Sbjct: 307 RVTGVAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHY 366

Query: 364 GSINITRTIKLFN-SVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDE 423
           G INIT T  L +   + ++G LR  +NG+S ++P TP++ A+   V      Y +   +
Sbjct: 367 GQINITNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKG---AYKLDFPD 426

Query: 424 GPPEGSTSITLAPNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKR 483
            P   +  + L  +++N T++ F++++F+N +  IQS+H++GYSFF V ++ G WS DK+
Sbjct: 427 RP--FNRPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKK 486

Query: 484 SNYNLLDAVSRHTIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALS 543
            +YN  DA+SR TI+VYP  W A+ ++ DN G+WN+R E  +  YLG++ Y+ + +P   
Sbjct: 487 GSYNNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEED 546

Query: 544 LRDEYNIPDNVLLCGLVKGMPLPKPYS 556
            + E + PDNVL CG +K +   + +S
Sbjct: 547 GKTEMDPPDNVLYCGALKNLQKEQHHS 563

BLAST of CmoCh20G000010 vs. ExPASy TrEMBL
Match: A0A6J1FYK3 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111448771 PE=3 SV=1)

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP
Sbjct: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK
Sbjct: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK
Sbjct: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH
Sbjct: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII
Sbjct: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL
Sbjct: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA
Sbjct: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH
Sbjct: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL
Sbjct: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVKGMPLPKPYSI
Sbjct: 541 LCGLVKGMPLPKPYSI 556

BLAST of CmoCh20G000010 vs. ExPASy TrEMBL
Match: A0A6J1JED3 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111483763 PE=3 SV=1)

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 542/556 (97.48%), Postives = 550/556 (98.92%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           MGR+MFSALLQ LCLSSVAVWTVRGEDPY FFVWNVSYGTISPLGVPQQAILINGQFPGP
Sbjct: 1   MGRLMFSALLQFLCLSSVAVWTVRGEDPYLFFVWNVSYGTISPLGVPQQAILINGQFPGP 60

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNNV+VNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK
Sbjct: 61  NINSTSNNNVVVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDW+TKSHTTLRK
Sbjct: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWFTKSHTTLRK 180

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILDSG RSIARPNGVLINGKIAKGDGTD+PLFTMKPGKTYKYRICNVGLKASLNLRFQGH
Sbjct: 181 ILDSGVRSIARPNGVLINGKIAKGDGTDDPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           TMKLVEMEGSHTVQN+YQSLDVHVGQCFSVLVTADQEPKDYYLVASTRF+KNVLVGKGII
Sbjct: 241 TMKLVEMEGSHTVQNEYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFIKNVLVGKGII 300

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL
Sbjct: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYF + DKVFKYNIISDEGPPEGSTSITLA
Sbjct: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFRLSDKVFKYNIISDEGPPEGSTSITLA 420

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVNVTFRNFVEIIFENREKSIQSWHL+GYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH
Sbjct: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLDGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQVYPNSWAAIFLTFDNAGMWNLRSEL EDRYLGQQLYISVLSPALSLRDEYNIPDNVL
Sbjct: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELIEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVKGMPLPKPYSI
Sbjct: 541 LCGLVKGMPLPKPYSI 556

BLAST of CmoCh20G000010 vs. ExPASy TrEMBL
Match: A0A5A7TF08 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G001020 PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.4e-301
Identity = 484/556 (87.05%), Postives = 522/556 (93.88%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           M  +MF+    LLCLS+  +  VRGEDPYFFF WNV+YGTISPLGVPQQ ILINGQFPGP
Sbjct: 2   MATMMFTV---LLCLSAATMSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGP 61

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNN++VNVFNNL+EPFLLHW+GIQHRKNSWQDG LGTNCPIPPG N+TY FQVK
Sbjct: 62  NINSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVK 121

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSI +HRAAG FGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTL++
Sbjct: 122 DQIGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQ 181

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILD+G RSIARP GVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK SLN RFQGH
Sbjct: 182 ILDNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGH 241

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA+QEPKDYY+VASTRF+KNV++GKGII
Sbjct: 242 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGII 301

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIP+AP GW WSLNQFR+FRWNLTASAARPNPQGSY YGSINITRTIKL
Sbjct: 302 RYTNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKL 361

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNS +KVDGKLRYAINGVSHIDPETPLK AEYFG+ DKVFKY+IISDEGP EG+T++T+A
Sbjct: 362 FNSATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDIISDEGPTEGATAVTIA 421

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVN TFRNF+EIIFEN EKS+QSWHL+GYSFFAVAIEPGRWSP+KRSNYNLLDAVSRH
Sbjct: 422 PNVVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRH 481

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQV+PNSWAAI LTFDNAGMWNLRSELTE+RYLGQQLY+SVLSPA SLRDEYNIPD+ L
Sbjct: 482 TIQVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTL 541

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVKGMPLPKPY+I
Sbjct: 542 LCGLVKGMPLPKPYTI 554

BLAST of CmoCh20G000010 vs. ExPASy TrEMBL
Match: A0A1S3BNS2 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103491644 PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.4e-301
Identity = 484/556 (87.05%), Postives = 522/556 (93.88%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           M  +MF+    LLCLS+  +  VRGEDPYFFF WNV+YGTISPLGVPQQ ILINGQFPGP
Sbjct: 1   MATMMFTV---LLCLSAATMSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGP 60

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNN++VNVFNNL+EPFLLHW+GIQHRKNSWQDG LGTNCPIPPG N+TY FQVK
Sbjct: 61  NINSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVK 120

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSI +HRAAG FGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTL++
Sbjct: 121 DQIGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQ 180

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILD+G RSIARP GVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK SLN RFQGH
Sbjct: 181 ILDNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGH 240

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA+QEPKDYY+VASTRF+KNV++GKGII
Sbjct: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGII 300

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIP+AP GW WSLNQFR+FRWNLTASAARPNPQGSY YGSINITRTIKL
Sbjct: 301 RYTNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKL 360

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNS +KVDGKLRYAINGVSHIDPETPLK AEYFG+ DKVFKY+IISDEGP EG+T++T+A
Sbjct: 361 FNSATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDIISDEGPTEGATAVTIA 420

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVN TFRNF+EIIFEN EKS+QSWHL+GYSFFAVAIEPGRWSP+KRSNYNLLDAVSRH
Sbjct: 421 PNVVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRH 480

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQV+PNSWAAI LTFDNAGMWNLRSELTE+RYLGQQLY+SVLSPA SLRDEYNIPD+ L
Sbjct: 481 TIQVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTL 540

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVKGMPLPKPY+I
Sbjct: 541 LCGLVKGMPLPKPYTI 553

BLAST of CmoCh20G000010 vs. ExPASy TrEMBL
Match: A0A5D3DE75 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001200 PE=3 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.4e-301
Identity = 483/556 (86.87%), Postives = 522/556 (93.88%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           M  +MF+    LLCLS+  +  VRGEDPYFFF WNV+YGTISPLGVPQQ ILINGQFPGP
Sbjct: 2   MATMMFTV---LLCLSAATMSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGP 61

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNN++VNVFNNL+EPFLLHW+GIQHRKNSWQDG LGTNCPIPPG N+TY FQVK
Sbjct: 62  NINSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVK 121

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSI +HRAAG FGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTL++
Sbjct: 122 DQIGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQ 181

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILD+G RSIARP GVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK SLN RFQGH
Sbjct: 182 ILDNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGH 241

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA+QEPKDYY+VASTRF+KNV++GKGII
Sbjct: 242 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGII 301

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIP+AP GW WSLNQFR+FRWNLTASAARPNPQGSY YGSINITRTIKL
Sbjct: 302 RYTNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKL 361

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNS +KVDGKLRYAINGVSH+DPETPLK AEYFG+ DKVFKY+IISDEGP EG+T++T+A
Sbjct: 362 FNSATKVDGKLRYAINGVSHVDPETPLKLAEYFGMADKVFKYDIISDEGPTEGATAVTIA 421

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVN TFRNF+EIIFEN EKS+QSWHL+GYSFFAVAIEPGRWSP+KRSNYNLLDAVSRH
Sbjct: 422 PNVVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRH 481

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQV+PNSWAAI LTFDNAGMWNLRSELTE+RYLGQQLY+SVLSPA SLRDEYNIPD+ L
Sbjct: 482 TIQVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTL 541

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVKGMPLPKPY+I
Sbjct: 542 LCGLVKGMPLPKPYTI 554

BLAST of CmoCh20G000010 vs. NCBI nr
Match: XP_022944275.1 (L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP
Sbjct: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK
Sbjct: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK
Sbjct: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH
Sbjct: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII
Sbjct: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL
Sbjct: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA
Sbjct: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH
Sbjct: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL
Sbjct: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVKGMPLPKPYSI
Sbjct: 541 LCGLVKGMPLPKPYSI 556

BLAST of CmoCh20G000010 vs. NCBI nr
Match: KAG6570306.1 (L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 552/556 (99.28%), Postives = 555/556 (99.82%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP
Sbjct: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNNVIVN+FNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK
Sbjct: 61  NINSTSNNNVIVNIFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK
Sbjct: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH
Sbjct: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII
Sbjct: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL
Sbjct: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNSVSKVDGKLRYAINGVSH+DPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA
Sbjct: 361 FNSVSKVDGKLRYAINGVSHVDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVNVTFRNFVEIIFENREKSIQSWHL+GYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH
Sbjct: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLDGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL
Sbjct: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVK MPLPKPYSI
Sbjct: 541 LCGLVKDMPLPKPYSI 556

BLAST of CmoCh20G000010 vs. NCBI nr
Match: XP_023513087.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 549/556 (98.74%), Postives = 555/556 (99.82%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           MGRV+FSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP
Sbjct: 1   MGRVIFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK
Sbjct: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK
Sbjct: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH
Sbjct: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           +MKLVEMEGSHTVQN+YQSLDVHVGQCFSVLVTADQEPKDYYLVASTRF+KNVLVGKGII
Sbjct: 241 SMKLVEMEGSHTVQNEYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFIKNVLVGKGII 300

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL
Sbjct: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA
Sbjct: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVNVTFRNFVEIIFENREKSIQSWHL+GYSFFAVAIEPGRWSP+KRSNYNLLDAVSRH
Sbjct: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRH 480

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQVYPNSWAAIFLTFDNAGMWNLRSEL EDRYLGQQLYISVLSPALSLRDEYNIPDNVL
Sbjct: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELIEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVKGMPLPKPYSI
Sbjct: 541 LCGLVKGMPLPKPYSI 556

BLAST of CmoCh20G000010 vs. NCBI nr
Match: XP_022985834.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 542/556 (97.48%), Postives = 550/556 (98.92%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           MGR+MFSALLQ LCLSSVAVWTVRGEDPY FFVWNVSYGTISPLGVPQQAILINGQFPGP
Sbjct: 1   MGRLMFSALLQFLCLSSVAVWTVRGEDPYLFFVWNVSYGTISPLGVPQQAILINGQFPGP 60

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNNV+VNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK
Sbjct: 61  NINSTSNNNVVVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDW+TKSHTTLRK
Sbjct: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWFTKSHTTLRK 180

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILDSG RSIARPNGVLINGKIAKGDGTD+PLFTMKPGKTYKYRICNVGLKASLNLRFQGH
Sbjct: 181 ILDSGVRSIARPNGVLINGKIAKGDGTDDPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           TMKLVEMEGSHTVQN+YQSLDVHVGQCFSVLVTADQEPKDYYLVASTRF+KNVLVGKGII
Sbjct: 241 TMKLVEMEGSHTVQNEYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFIKNVLVGKGII 300

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL
Sbjct: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYF + DKVFKYNIISDEGPPEGSTSITLA
Sbjct: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFRLSDKVFKYNIISDEGPPEGSTSITLA 420

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVNVTFRNFVEIIFENREKSIQSWHL+GYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH
Sbjct: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLDGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQVYPNSWAAIFLTFDNAGMWNLRSEL EDRYLGQQLYISVLSPALSLRDEYNIPDNVL
Sbjct: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELIEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVKGMPLPKPYSI
Sbjct: 541 LCGLVKGMPLPKPYSI 556

BLAST of CmoCh20G000010 vs. NCBI nr
Match: KAA0040127.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1043.9 bits (2698), Expect = 5.0e-301
Identity = 484/556 (87.05%), Postives = 522/556 (93.88%), Query Frame = 0

Query: 1   MGRVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGP 60
           M  +MF+    LLCLS+  +  VRGEDPYFFF WNV+YGTISPLGVPQQ ILINGQFPGP
Sbjct: 2   MATMMFTV---LLCLSAATMSMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGP 61

Query: 61  NINSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVK 120
           NINSTSNNN++VNVFNNL+EPFLLHW+GIQHRKNSWQDG LGTNCPIPPG N+TY FQVK
Sbjct: 62  NINSTSNNNIVVNVFNNLNEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVK 121

Query: 121 DQIGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRK 180
           DQIGSFFYYPSI +HRAAG FGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTL++
Sbjct: 122 DQIGSFFYYPSIAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQ 181

Query: 181 ILDSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGH 240
           ILD+G RSIARP GVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK SLN RFQGH
Sbjct: 182 ILDNGHRSIARPEGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGH 241

Query: 241 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGII 300
           TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTA+QEPKDYY+VASTRF+KNV++GKGII
Sbjct: 242 TMKLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGII 301

Query: 301 RYTNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKL 360
           RYTNGKGPASPEIP+AP GW WSLNQFR+FRWNLTASAARPNPQGSY YGSINITRTIKL
Sbjct: 302 RYTNGKGPASPEIPKAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKL 361

Query: 361 FNSVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLA 420
           FNS +KVDGKLRYAINGVSHIDPETPLK AEYFG+ DKVFKY+IISDEGP EG+T++T+A
Sbjct: 362 FNSATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDIISDEGPTEGATAVTIA 421

Query: 421 PNVVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRH 480
           PNVVN TFRNF+EIIFEN EKS+QSWHL+GYSFFAVAIEPGRWSP+KRSNYNLLDAVSRH
Sbjct: 422 PNVVNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRH 481

Query: 481 TIQVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVL 540
           TIQV+PNSWAAI LTFDNAGMWNLRSELTE+RYLGQQLY+SVLSPA SLRDEYNIPD+ L
Sbjct: 482 TIQVFPNSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTL 541

Query: 541 LCGLVKGMPLPKPYSI 557
           LCGLVKGMPLPKPY+I
Sbjct: 542 LCGLVKGMPLPKPYTI 554

BLAST of CmoCh20G000010 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 888.6 bits (2295), Expect = 2.5e-258
Identity = 405/547 (74.04%), Postives = 459/547 (83.91%), Query Frame = 0

Query: 9   LLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNINSTSNN 68
           L   L L++ A   VR EDPYF  VWNV+YGT+SPLGVPQQ ILINGQFPGPN+NSTSNN
Sbjct: 7   LAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVNSTSNN 66

Query: 69  NVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQIGSFFY 128
           NVI+NVFNNLDEPFLL WNGIQHRKN WQDG  GT CPI PGTNYTY FQ KDQIGS+FY
Sbjct: 67  NVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFY 126

Query: 129 YPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKILDSGGRS 188
           YPS  +HR+AG FGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHT L+K LDS GR+
Sbjct: 127 YPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDS-GRT 186

Query: 189 IARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHTMKLVEME 248
           + RP+G+LINGK  KGDG+D PLFT+KPGKTY+ RICNVGLK SLN R Q H +KLVEME
Sbjct: 187 LGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLKLVEME 246

Query: 249 GSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGIIRYTNGKGP 308
           GSH +QNDY SLDVHVGQC+  ++TA+QE KDYY+VAS+RF+K+V+   G++RY  GKGP
Sbjct: 247 GSHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYEGGKGP 306

Query: 309 ASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKLFNSVSKVD 368
           AS ++P  P GW WSLNQFRSFRWNLTASAARPNPQGSY YG INITRTIKL N+  KVD
Sbjct: 307 ASSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVD 366

Query: 369 GKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLAPNVVNVTF 428
           GKLRYA+NGVSH DPETPLK AEYFGV DKVFKY+ I+D   PE   SI + PNV+N+T 
Sbjct: 367 GKLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNVLNITH 426

Query: 429 RNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRHTIQVYPNS 488
           R F+E++FEN EKS+QSWHL+GYSFFAVA+EPG W+P+KR NYNLLDAVSRHT+QVYP  
Sbjct: 427 RTFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKC 486

Query: 489 WAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVLLCGLVKGM 548
           WAAI LTFDN GMWN+RSE +E RYLGQQLY SVLSP  SLRDEYN+P+  L CGLVKG 
Sbjct: 487 WAAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGT 546

Query: 549 PLPKPYS 556
           P P PY+
Sbjct: 547 PKPNPYA 552

BLAST of CmoCh20G000010 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 885.6 bits (2287), Expect = 2.1e-257
Identity = 403/543 (74.22%), Postives = 456/543 (83.98%), Query Frame = 0

Query: 13  LCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNINSTSNNNVIV 72
           LC+++  V  V+ EDPYF  VWNV+YGT SPLGVPQQ ILINGQFPGPNINSTSNNNVIV
Sbjct: 12  LCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVIV 71

Query: 73  NVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQIGSFFYYPSI 132
           NVFNNLDEPFL+ W GIQHRKN WQDG  GT CPIPPG N+TY FQ KDQIGS+FYYP+ 
Sbjct: 72  NVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQIGSYFYYPTT 131

Query: 133 GLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKILDSGGRSIARP 192
            +HRAAG FGGLR+NSRLLIPVPYADPEDDYT+LI DWYTKSHT L+K LDS GR+I RP
Sbjct: 132 AMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDS-GRTIGRP 191

Query: 193 NGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHTMKLVEMEGSHT 252
           +G+LINGK  K DG+D+PLFT+KPGKTY+ RICNVGLKASLN R Q H MKLVEMEGSH 
Sbjct: 192 DGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLVEMEGSHV 251

Query: 253 VQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGIIRYTNGKGPASPE 312
           +QNDY SLDVHVGQCF V+VTADQEPKDYY++ASTRF+K  L   G++RY  GKGPAS +
Sbjct: 252 LQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGGKGPASSQ 311

Query: 313 IPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKLFNSVSKVDGKLR 372
           +P AP GW WSLNQ+RSFRWNLTASAARPNPQGSY YG INITRTIKL N+  KVDGKLR
Sbjct: 312 LPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKLR 371

Query: 373 YAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLAPNVVNVTFRNFV 432
           YA++GVSH DPETPLK AEYFGV DKVFKY+ ISD   P+   +I + PNV+N+T R F+
Sbjct: 372 YALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLNITHRTFI 431

Query: 433 EIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRHTIQVYPNSWAAI 492
           E++FEN E+S+QSWHL+GYSFFAVA+EPG W+P+KR NYNLLDAVSRHT+QVYP  WAAI
Sbjct: 432 EVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAI 491

Query: 493 FLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVLLCGLVKGMPLPK 552
            LTFDN GMWN+RSE  E RYLGQQLY SVLSP  SLRDEYN+P+  L CGLVKG P   
Sbjct: 492 LLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGKPKVN 551

Query: 553 PYS 556
           PY+
Sbjct: 552 PYA 553

BLAST of CmoCh20G000010 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 852.4 bits (2201), Expect = 2.0e-247
Identity = 394/548 (71.90%), Postives = 457/548 (83.39%), Query Frame = 0

Query: 9   LLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNINSTSNN 68
           L  L+CL+S  V  V   DPYF++ WNV+YGT +PLG+PQQ ILINGQFPGPN+NSTSNN
Sbjct: 7   LTVLVCLAS-TVALVSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNSTSNN 66

Query: 69  NVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQIGSFFY 128
           NV++NVFNNLDEPFLL W+G+QHRKNSWQDG  GT+CPIP GTN+TY FQ KDQIGS+FY
Sbjct: 67  NVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIGSYFY 126

Query: 129 YPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKILDSGGRS 188
           YPS  LHR AG FGGLR+NSRLLIPVPYADPEDD T+LI DWY KSHT L+  LDS GR+
Sbjct: 127 YPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDS-GRT 186

Query: 189 IARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHTMKLVEME 248
           +  P+GVLINGK  K  G + PLFTMKPGKTYKYRICNVG K++LN R QGH MKLVEME
Sbjct: 187 LGSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLVEME 246

Query: 249 GSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGIIRYTNGKGP 308
           GSH +QNDY SLDVHVGQCF+VLVTADQ  K+YY+VASTRF+K  +   G++ Y      
Sbjct: 247 GSHVLQNDYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGSNVQ 306

Query: 309 ASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKLFNSVSKVD 368
           AS +IP+AP GW WSLNQFRSFRWNLTASAARPNPQGSY YG INITRTIKL N+ + V+
Sbjct: 307 ASSDIPKAPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKNLVN 366

Query: 369 GKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLAPNVVNVTF 428
           GK+R+  NGVSH+D ETPLK AEYFG+ +KVFKYN+I DE P    T++T+ PNV+N+TF
Sbjct: 367 GKVRFGFNGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDE-PAAKITTLTVEPNVLNITF 426

Query: 429 RNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRHTIQVYPNS 488
           R FVE++FEN EKS+QS+HL+GYSFFAVA EPGRW+P+KR+NYNLLDAVSRHT+QVYP S
Sbjct: 427 RTFVEVVFENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYPKS 486

Query: 489 WAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVLLCGLVKGM 548
           W+AI LTFDNAGMWN+RSE  E RYLGQQLY+SVLSP  SLRDEYNIP N  LCG+VKG+
Sbjct: 487 WSAILLTFDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGL 546

Query: 549 PLPKPYSI 557
           PLP PY+I
Sbjct: 547 PLPTPYTI 551

BLAST of CmoCh20G000010 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 847.8 bits (2189), Expect = 4.9e-246
Identity = 396/548 (72.26%), Postives = 454/548 (82.85%), Query Frame = 0

Query: 9   LLQLLCL-SSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNINSTSN 68
           L  L+CL S+VA+  V   DPYFF  WNV+YGT SPLGVPQ+ ILINGQFPGPN+NSTSN
Sbjct: 6   LTVLVCLVSTVAI--VNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLNSTSN 65

Query: 69  NNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQIGSFF 128
           NNV++NVFN+LDEPFLL W+GIQHRKN WQDG  GT+CPIP G N+TY FQ KDQIGS+F
Sbjct: 66  NNVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQIGSYF 125

Query: 129 YYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKILDSGGR 188
           YYP+  LHR AG FGGLR+NSRLLIPVPYADPEDDYTVL+GDWYT  HT L+  LDS GR
Sbjct: 126 YYPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLDS-GR 185

Query: 189 SIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHTMKLVEM 248
           ++  PNGVLINGK  K  G +EPLFTMKPGKTYKYR+CNVG K++LN R Q H MKLVEM
Sbjct: 186 TLGLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKLVEM 245

Query: 249 EGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGIIRYTNGKG 308
           EGSH +QNDY SLDVHVGQCFSVLVTA+Q  KDYY+VASTRF+K  L   G+IRY     
Sbjct: 246 EGSHVIQNDYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEGSNV 305

Query: 309 PASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKLFNSVSKV 368
            AS E+P+AP GW WSLNQFRSFRWNLT++AARPNPQGSY YG INITR+IKL NS S V
Sbjct: 306 QASTELPKAPVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSKSVV 365

Query: 369 DGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLAPNVVNVT 428
           DGK+R+  NGVSH+D ETPLK AEYF + +KVFKYN+I DE P    T++T+ PNV+N+T
Sbjct: 366 DGKVRFGFNGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDE-PAAKITALTVQPNVLNIT 425

Query: 429 FRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRHTIQVYPN 488
           FR FVEIIFEN EK++QS+HL+GYSFFAVA EPGRW+P+KR NYNLLDAVSRHT+QVYP 
Sbjct: 426 FRTFVEIIFENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQVYPK 485

Query: 489 SWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVLLCGLVKG 548
           SW+AI LTFDNAGMWN+RSE  E +YLG+QLY+SVLSP  SLRDEYNIP N  LCG+VKG
Sbjct: 486 SWSAILLTFDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKG 545

Query: 549 MPLPKPYS 556
           +PLP  YS
Sbjct: 546 LPLPAHYS 549

BLAST of CmoCh20G000010 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 638.3 bits (1645), Expect = 5.9e-183
Identity = 308/541 (56.93%), Postives = 388/541 (71.72%), Query Frame = 0

Query: 3   RVMFSALLQLLCLSSVAVWTVRGEDPYFFFVWNVSYGTISPLGVPQQAILINGQFPGPNI 62
           R M + LL L+ L+         EDPY FF W+V+YG ISPLGV QQ ILING+FPGP+I
Sbjct: 9   RAMITTLLFLISLAF-------AEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDI 68

Query: 63  NSTSNNNVIVNVFNNLDEPFLLHWNGIQHRKNSWQDGQLGTNCPIPPGTNYTYRFQVKDQ 122
            S +N+N+I+NVFN+LDEPFLL WNGI++ KNS+QDG  GT CPIPPG NYTY  QVKDQ
Sbjct: 69  ISITNDNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQ 128

Query: 123 IGSFFYYPSIGLHRAAGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLRKIL 182
           IGSF+Y+PS+G H+AAG FGG+RI+SR LIPVP+  P DDYT+L+GDWY  +H  L+  L
Sbjct: 129 IGSFYYFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQL 188

Query: 183 DSGGRSIARPNGVLINGKIAKGDGTDEPLFTMKPGKTYKYRICNVGLKASLNLRFQGHTM 242
           D+GG+ +  P+G+LING+      +      ++PGKTY+ RI NVGL+ SLN R Q HTM
Sbjct: 189 DNGGK-LPLPDGILINGR------SSGATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTM 248

Query: 243 KLVEMEGSHTVQNDYQSLDVHVGQCFSVLVTADQEPKDYYLVASTRFMKNVLVGKGIIRY 302
           KLVE+EG +T+QN + SLDVHVGQ +SVL+TADQ  KDYY+V S+RF   +L   G++ Y
Sbjct: 249 KLVEVEGRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHY 308

Query: 303 TNGKGPASPEIPEAPTGWIWSLNQFRSFRWNLTASAARPNPQGSYRYGSINITRTIKLFN 362
           +N   P S  IP+ P    WS NQ R+ R NLTAS  RPNPQGSYRYG INITRTI+L N
Sbjct: 309 SNSVAPVSGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLAN 368

Query: 363 SVSKVDGKLRYAINGVSHIDPETPLKHAEYFGVGDKVFKYNIISDEGPPEGSTSITLAPN 422
           ++  ++GK RYA+N  S    +TPLK  +YF + D V+K   ISD+ P  G  +I    +
Sbjct: 369 NLGHIEGKQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQ-PTNG--AIFPTTS 428

Query: 423 VVNVTFRNFVEIIFENREKSIQSWHLNGYSFFAVAIEPGRWSPDKRSNYNLLDAVSRHTI 482
           V+   FR FVE+IFEN E  +QSWHL+GYSF+ V +E G+WSP  R  YNL DA+ R TI
Sbjct: 429 VMQADFRAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTI 488

Query: 483 QVYPNSWAAIFLTFDNAGMWNLRSELTEDRYLGQQLYISVLSPALSLRDEYNIPDNVLLC 542
           QVYP SW AI++  DN GMWN+RSE+ E +YLGQQ Y+ V + + SLRDEY IP N LLC
Sbjct: 489 QVYPRSWTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLC 531

Query: 543 G 544
           G
Sbjct: 549 G 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006245.6e-25573.54L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291623.3e-23969.30L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU401.4e-15749.36Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q9FHN64.9e-15047.80Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Q8VXX51.1e-14646.03Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1FYK30.0e+00100.00L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111448771 PE=3 S... [more]
A0A6J1JED30.0e+0097.48L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111483763 PE=3 SV=... [more]
A0A5A7TF082.4e-30187.05L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3BNS22.4e-30187.05L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103491644 PE=3 SV=1[more]
A0A5D3DE755.4e-30186.87L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
XP_022944275.10.0e+00100.00L-ascorbate oxidase homolog [Cucurbita moschata][more]
KAG6570306.10.0e+0099.28L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023513087.10.0e+0098.74L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo][more]
XP_022985834.10.0e+0097.48L-ascorbate oxidase homolog [Cucurbita maxima][more]
KAA0040127.15.0e-30187.05L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G13390.12.5e-25874.04SKU5 similar 11 [more]
AT1G55570.12.1e-25774.22SKU5 similar 12 [more]
AT3G13400.12.0e-24771.90SKU5 similar 13 [more]
AT1G55560.14.9e-24672.26SKU5 similar 14 [more]
AT1G21860.15.9e-18356.93SKU5 similar 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 406..523
e-value: 1.5E-23
score: 83.1
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 36..149
e-value: 1.9E-40
score: 137.6
IPR008972CupredoxinGENE3D2.60.40.420coord: 159..340
e-value: 7.3E-36
score: 126.1
IPR008972CupredoxinGENE3D2.60.40.420coord: 350..548
e-value: 2.4E-34
score: 120.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 31..150
e-value: 4.3E-34
score: 119.3
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 25..176
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 158..341
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 352..542
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 161..302
e-value: 4.8E-39
score: 134.0
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 7..547
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 7..547
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 161..302
e-value: 3.72419E-83
score: 253.479

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G000010.1CmoCh20G000010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity