CmoCh19G009320 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh19G009320
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRNase III domain-containing protein
LocationCmo_Chr19: 8609819 .. 8611737 (+)
RNA-Seq ExpressionCmoCh19G009320
SyntenyCmoCh19G009320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACACTTACAGAAAAAGAATAGACGGAATTAAATTAAAATTCGTTCCAGAATCCCTAAATTTCCTTCATGGAAGGAGGGGGAGACGGCGGCGATTTGTTGAAGCAGGAAGGCAGAGGCGGCGAGTCAACGGCGGCGAGTCTGGACGGAATGGAAGCTATCCTTAAGTACGAATTTCGAGACAAGAGATTGCTGGAGGAGGCTTTCACAGACGCGTCGTATTCGCCGGAAAATTGCTCGTCGTTCGAGCGATTGGAGTACGTAGGCGATTCGGTGTTGAATTTTCTAATAACCAGAGAGCAATACTTCTCGAATCCTAATCTGTCGCCCGGTACACTGACTCGTCTCCGAGCGGCGAATGTGGACACCGAAAAGCTGGCTCGTGTTGCGATTACACATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTGATCAGAAAGTAAGTACCGATTGTTTTGATTTTTCATCGTTTTCATCGATTTCTCTGGATTTCCTCTGTTTTCTTCTTTCATAATTATGATCATTAACTGTTAACCAACCCACTAATTACGATATTTCTTTTCTCCTTCTCCTTCTTCAATTTTATATCTTTTTAAAAATTTTCAATTCTTAAATTTTTGTTTCAAAATCTTAAATCTTAAATATATTTTTTATCGCCTAAATTTAATTCAAACAATTTGAGTATTTGATTAATAATTTATGATTTATTAAAATTATATGAACTAAAATTTAGATATTTGAAAATATAATTAATTCGATTTTAATCATAAATTTTTTCCATAACCATTATTTTTTAAATATAATTTCGTTCTTTTTTCTAAGGTTTTTTTTTTCCTTTTCCAAAATCTTTATTTTATATATATATATTTTTGTGCAATTTTAGTAGTAAAATCGGAGAGAAATATATTCCATCTCTTTCCTAAAATTGCTTTTGAATTTTGTTCTTTTTGGAAAAACCTCTTATGTTTGATTTAAATTAGTCAATATTCGTTTAATTCTTTTTATATAAAGGTAAACAATTTTTTTAGTTGATTCAACGAAATATAAAGTGACAGTAATAGTAATTAATTAATTAAAAATGCGTGATAATTCATGAAAATTAATTGCGGTTTAGGGCATGCTCTTACATTTATTCTTTTGAAATATTTGAATCTTATTAATTAACGTGAAAATTAAATGTAGCGTTAATTGTTTTGAAACCCTATTTCTTCTATTATTTTATTAAAATTAAATTTTAAAAATCATTTGTTAAAAAAAAACTATTATAGGTTAAAATAGAATAATTTATTTTTTCCCTTTTTGTCTTTGTCTTTTTTTTTTTTGGAATAACATGTAAATCCAATTTTTAATTTAAATTTTGTGTGTGCGACGACAGATAGAAGAGTTTAGTAAGAATATAGAGGAGTACCCATTGCATTCGAACGGACTGATTAAAGCTCCAAAGATTCTAGCGGACATAGTTGAATCGTGCATCGGAGCAGTTTTCACAGATAGTAACTCGATCGACACAGTTTGGACGGTAAATACATTTAAAGAAGAAGAAGGATGAAAATTTTGAGTTATAATGGTAAGTTTTGATTGAAGTATAGGATGGTTTGATTTGTGTAGATATTCAAGGGTCTAATGGAGCCATTGATAACATTGAAGACCATGAAAAGGCACCCGATGACTGAGCTGACTGAAATGTGTCAAAAAAGGAACCTGAAGTGGGAATTCGTGGACTTGTGGGAAGAAAGAAAGGAGATCCACGTTTTGATAGACAAGCAGCTGGTTGGAAAAGGAAGCTATGGGAAGAAGCTCATTGCACAAAATCGAGCTGCAAAAGATGCTTTGGATAACTTCCACACAATTCTTGGCCAATTTAATGAACCTTGTCAACCTATGTAATCTATTCCTAATTATAATTACTCCCCC

mRNA sequence

ACACTTACAGAAAAAGAATAGACGGAATTAAATTAAAATTCGTTCCAGAATCCCTAAATTTCCTTCATGGAAGGAGGGGGAGACGGCGGCGATTTGTTGAAGCAGGAAGGCAGAGGCGGCGAGTCAACGGCGGCGAGTCTGGACGGAATGGAAGCTATCCTTAAGTACGAATTTCGAGACAAGAGATTGCTGGAGGAGGCTTTCACAGACGCGTCGTATTCGCCGGAAAATTGCTCGTCGTTCGAGCGATTGGAGTACGTAGGCGATTCGGTGTTGAATTTTCTAATAACCAGAGAGCAATACTTCTCGAATCCTAATCTGTCGCCCGGTACACTGACTCGTCTCCGAGCGGCGAATGTGGACACCGAAAAGCTGGCTCGTGTTGCGATTACACATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTGATCAGAAAATAGAAGAGTTTAGTAAGAATATAGAGGAGTACCCATTGCATTCGAACGGACTGATTAAAGCTCCAAAGATTCTAGCGGACATAGTTGAATCGTGCATCGGAGCAGTTTTCACAGATAGTAACTCGATCGACACAGTTTGGACGATATTCAAGGGTCTAATGGAGCCATTGATAACATTGAAGACCATGAAAAGGCACCCGATGACTGAGCTGACTGAAATGTGTCAAAAAAGGAACCTGAAGTGGGAATTCGTGGACTTGTGGGAAGAAAGAAAGGAGATCCACGTTTTGATAGACAAGCAGCTGGTTGGAAAAGGAAGCTATGGGAAGAAGCTCATTGCACAAAATCGAGCTGCAAAAGATGCTTTGGATAACTTCCACACAATTCTTGGCCAATTTAATGAACCTTGTCAACCTATGTAATCTATTCCTAATTATAATTACTCCCCC

Coding sequence (CDS)

ATGGAAGGAGGGGGAGACGGCGGCGATTTGTTGAAGCAGGAAGGCAGAGGCGGCGAGTCAACGGCGGCGAGTCTGGACGGAATGGAAGCTATCCTTAAGTACGAATTTCGAGACAAGAGATTGCTGGAGGAGGCTTTCACAGACGCGTCGTATTCGCCGGAAAATTGCTCGTCGTTCGAGCGATTGGAGTACGTAGGCGATTCGGTGTTGAATTTTCTAATAACCAGAGAGCAATACTTCTCGAATCCTAATCTGTCGCCCGGTACACTGACTCGTCTCCGAGCGGCGAATGTGGACACCGAAAAGCTGGCTCGTGTTGCGATTACACATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTGATCAGAAAATAGAAGAGTTTAGTAAGAATATAGAGGAGTACCCATTGCATTCGAACGGACTGATTAAAGCTCCAAAGATTCTAGCGGACATAGTTGAATCGTGCATCGGAGCAGTTTTCACAGATAGTAACTCGATCGACACAGTTTGGACGATATTCAAGGGTCTAATGGAGCCATTGATAACATTGAAGACCATGAAAAGGCACCCGATGACTGAGCTGACTGAAATGTGTCAAAAAAGGAACCTGAAGTGGGAATTCGTGGACTTGTGGGAAGAAAGAAAGGAGATCCACGTTTTGATAGACAAGCAGCTGGTTGGAAAAGGAAGCTATGGGAAGAAGCTCATTGCACAAAATCGAGCTGCAAAAGATGCTTTGGATAACTTCCACACAATTCTTGGCCAATTTAATGAACCTTGTCAACCTATGTAA

Protein sequence

MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQFNEPCQPM
Homology
BLAST of CmoCh19G009320 vs. ExPASy Swiss-Prot
Match: Q69KJ0 (Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0358800 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 7.2e-59
Identity = 119/236 (50.42%), Postives = 164/236 (69.49%), Query Frame = 0

Query: 28  MEAILKYEFRDKRLLEEAFTDAS--YSPENCSSFERLEYVGDSVLNFLITREQYFSNPNL 87
           +E++L YEFRDK L+EEA T  S  Y      ++ERLEY+GD+VL  +++RE + +   L
Sbjct: 42  VESLLGYEFRDKALVEEALTHGSFYYPYRPGVTYERLEYLGDAVLTCVVSREVFLTYGQL 101

Query: 88  SPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNGLI 147
            PG LTRLRAANVD EKLARVA+ HG H +LRH   +LD +I +F + +  YP+HSNGL+
Sbjct: 102 QPGPLTRLRAANVDKEKLARVAVVHGLHHFLRHKAPNLDGQITDFIEELSMYPIHSNGLL 161

Query: 148 KAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQKR 207
             PK+L D+VES IGA++ DSN + + VW +F+ L +PLI+L+T+ +HP++EL E CQK 
Sbjct: 162 DPPKVLCDVVESLIGAIYCDSNFNQEIVWQVFQKLADPLISLETLGKHPVSELFEFCQKT 221

Query: 208 NLKWEFV-DLWEERKEIHVLIDKQLVGKGSYG-KKLIAQNRAAKDALDNFHTILGQ 259
               + V D W++   + VLID ++VG+ +Y  KK IAQNRAAK ALD     LGQ
Sbjct: 222 RRGVKIVKDEWDKNLTVEVLIDGEMVGRATYAQKKEIAQNRAAKAALDKLKETLGQ 277

BLAST of CmoCh19G009320 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 172.9 bits (437), Expect = 4.7e-42
Identity = 104/241 (43.15%), Postives = 145/241 (60.17%), Query Frame = 0

Query: 19   ESTAASLD--GMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITR 78
            ES   S+D  G+E  LKYEF++K LL EA T AS      S ++RLE+VGD+VL+ LITR
Sbjct: 1551 ESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITR 1610

Query: 79   EQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIE- 138
              +F+  +L PG LT LRAA V+ E  ARVA+ H  H YLRH  + L+++I EF K ++ 
Sbjct: 1611 HLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQT 1670

Query: 139  ---EYPLHSNGL--IKAPKILADIVESCIGAVFTDSNSIDT-VWTIFKGLMEPLITLKTM 198
               +   +S GL   KAPK+L DIVES  GA+F DS    T  W +F+ L++P++T +T+
Sbjct: 1671 ESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETL 1730

Query: 199  KRHPMTELTEMCQKRNLKWEF-VDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDA 250
              HP+ EL E CQ++    E+          + V ID   VG     +K +AQ  AA++A
Sbjct: 1731 PMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNA 1790

BLAST of CmoCh19G009320 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.2e-40
Identity = 94/237 (39.66%), Postives = 149/237 (62.87%), Query Frame = 0

Query: 20  STAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQY 79
           S   S++ +E IL Y+F +K LL+EA T  S +  +  S+ERLE++GDS +   I+   Y
Sbjct: 55  SEMESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAISNYLY 114

Query: 80  FSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNI---EE 139
            + P+L P  L+ LRAANV TEKLARV++ HG + +LR N   LD+K++EFS+ +   ++
Sbjct: 115 LTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDD 174

Query: 140 YPLHSNGLIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKR--HP 199
             +   GL+KAPK+LAD+ ES  GAV+ D N  +  +W IF+GL+EP++TL  +++   P
Sbjct: 175 LSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQP 234

Query: 200 MTELTEMCQKRNLKWEFVDLWEERKEIHVL-IDKQLVGKGSYGKKLIAQNRAAKDAL 250
           ++ L ++C K   + +  +  +    I V+ +D +L+  G    K IA+  AAK+AL
Sbjct: 235 VSMLFKLCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEAL 289

BLAST of CmoCh19G009320 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 2.0e-40
Identity = 93/231 (40.26%), Postives = 143/231 (61.90%), Query Frame = 0

Query: 28  MEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSP 87
           +E +L+Y+FRD RLLEEA T  S++ ++  S++RLE+VGDS L    +   Y +NP L P
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFA-DDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGP 99

Query: 88  GTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYP-------LH 147
           G L+ LRAAN+ TEKLARVA+ H  +  LR N   LD  + +F + +++ P        +
Sbjct: 100 GPLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPY 159

Query: 148 SNGLIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTELTE 207
              ++KAPK+LADIVE+   AV+ D    ++ +W + + L EP+IT +T+   P+T L E
Sbjct: 160 GGSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAETIDEQPVTMLHE 219

Query: 208 MCQKRNLKWEFVDLWEE--RKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDA 249
           +CQK     +F   W++     ++V +  +LVG GS  +K+IA+  AA+DA
Sbjct: 220 LCQKHGKMAQF-KTWQKGGMTVVNVFVAGELVGIGSSEQKVIAKLNAARDA 268

BLAST of CmoCh19G009320 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 6.4e-39
Identity = 96/234 (41.03%), Postives = 140/234 (59.83%), Query Frame = 0

Query: 24   SLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83
            + D ++ +L  EF++K LL EA T AS      S ++RLE+VGD+VL+ LITR  +F+  
Sbjct: 1537 NFDTLKGVLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYT 1596

Query: 84   NLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPL---- 143
            +L PG LT LRAA V+ E  ARVA+ H  H +LRH  + L+ +I EF K+++E  L    
Sbjct: 1597 DLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQEELLKPGF 1656

Query: 144  HSNGL--IKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTE 203
            +S GL   KAPK+L DIVES  GA+F DS      VW +F+ L+ P++T +T+  HP+ E
Sbjct: 1657 NSFGLGDCKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPETLPMHPVRE 1716

Query: 204  LTEMCQKRNLKWEF-VDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDAL 250
            L E CQ++    E+          + V +D   +G     +K +AQ  AA++AL
Sbjct: 1717 LQERCQQQAEGLEYKASRAGNIATVEVFVDGVQIGVAQNPQKKMAQKLAARNAL 1770

BLAST of CmoCh19G009320 vs. ExPASy TrEMBL
Match: A0A6J1GKQ4 (ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 3.5e-149
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 267
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

BLAST of CmoCh19G009320 vs. ExPASy TrEMBL
Match: A0A6J1I0C1 (ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 8.1e-146
Identity = 261/266 (98.12%), Postives = 263/266 (98.87%), Query Frame = 0

Query: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDG DLLKQEGR GESTAASLDGMEAIL YEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGVDLLKQEGRVGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNG+IKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGMIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 267
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

BLAST of CmoCh19G009320 vs. ExPASy TrEMBL
Match: A0A0A0K1J8 (RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.3e-116
Identity = 217/263 (82.51%), Postives = 231/263 (87.83%), Query Frame = 0

Query: 4   GGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLE 63
           G DG      E   G  + ASLDG+EAIL YEFRDKRLLEEAFTDASYSPENCSSFERLE
Sbjct: 13  GKDGSRGGGGEAVNGSDSPASLDGIEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLE 72

Query: 64  YVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADL 123
           YVGDSVLNFLITREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNKADL
Sbjct: 73  YVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADL 132

Query: 124 DQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPL 183
            QKIEEF ++IEEYPLHSNG+IKAPK+LADIVES IGAVF DSN IDTVW IFK LMEPL
Sbjct: 133 HQKIEEFGRSIEEYPLHSNGMIKAPKVLADIVESTIGAVFADSNFIDTVWKIFKELMEPL 192

Query: 184 ITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNR 243
           ITLKTMK+HPMTELTEMCQKR+LK EF D WEE KEI VLI+KQ+VGKGSY KKLIAQNR
Sbjct: 193 ITLKTMKKHPMTELTEMCQKRSLKLEFRDRWEETKEIEVLIEKQVVGKGSYRKKLIAQNR 252

Query: 244 AAKDALDNFHTILGQFNEPCQPM 267
           AAK+AL+N  T   QFN+PCQPM
Sbjct: 253 AAKNALENLDTFFPQFNQPCQPM 275

BLAST of CmoCh19G009320 vs. ExPASy TrEMBL
Match: A0A6J1C375 (ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.5e-115
Identity = 208/243 (85.60%), Postives = 228/243 (93.83%), Query Frame = 0

Query: 24  SLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83
           SL+ +EAIL YEFRDKRLLEEAFTD+SYSPENC SFERLEYVGDSVLNFLITREQYFSNP
Sbjct: 38  SLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNP 97

Query: 84  NLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143
           +LSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK+DLD+KIEEF++ ++EYPLHSNG
Sbjct: 98  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNG 157

Query: 144 LIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203
           LIKAPKILADIVES IGAVFTDSNS+DTVWTIF+GLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 158 LIKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQK 217

Query: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQFNEPC 263
           RNLK EFVD WE+ KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N  T+L Q N+PC
Sbjct: 218 RNLKLEFVDSWEKTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPC 277

Query: 264 QPM 267
           QPM
Sbjct: 278 QPM 280

BLAST of CmoCh19G009320 vs. ExPASy TrEMBL
Match: A0A1Q3BNT8 (Ribonuclease_3 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_13208 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 7.0e-81
Identity = 158/237 (66.67%), Postives = 192/237 (81.01%), Query Frame = 0

Query: 25  LDGMEAILKYEFRDKRLLEEAFTDASY-SPENCSSFERLEYVGDSVLNFLITREQYFSNP 84
           L+G+E IL Y+F +K LLEEAFT ASY SP+ C+S+ERLE+VGDSVLN L T+EQYF  P
Sbjct: 1   LEGVEEILGYKFGNKSLLEEAFTHASYTSPDQCASYERLEFVGDSVLNLLFTKEQYFLYP 60

Query: 85  NLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 144
           NL PG LTRLRAANVDTEKLARV+I HG HRYL H K  L+Q+I+EF++ I +YPLHSNG
Sbjct: 61  NLQPGDLTRLRAANVDTEKLARVSIRHGLHRYLCHKKPLLEQQIQEFTQAILDYPLHSNG 120

Query: 145 LIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQ 204
           L+  PK+LADIVES IGAVF DSN SID VW +FKGL+EP+I+++T+KRHP+TEL E+CQ
Sbjct: 121 LVDPPKVLADIVESTIGAVFIDSNLSIDIVWKVFKGLLEPIISVETLKRHPVTELYEVCQ 180

Query: 205 KRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYG-KKLIAQNRAAKDALDNFHTILGQ 259
           K+NLK +FVDLW+E     V ID QLVG+ +YG KK IA NRAAKDAL+N   IL +
Sbjct: 181 KKNLKVKFVDLWKESTAFEVFIDDQLVGRDTYGTKKEIAHNRAAKDALNNMWKILNE 237

BLAST of CmoCh19G009320 vs. NCBI nr
Match: XP_022952611.1 (ribonuclease 3-like protein 3 [Cucurbita moschata])

HSP 1 Score: 537.3 bits (1383), Expect = 7.3e-149
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 267
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

BLAST of CmoCh19G009320 vs. NCBI nr
Match: KAG7011897.1 (Ribonuclease 3-like protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 532.3 bits (1370), Expect = 2.3e-147
Identity = 264/266 (99.25%), Postives = 264/266 (99.25%), Query Frame = 0

Query: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEG GDGGDLLKQEGRGGESTAASLDGMEAIL YEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGRGDGGDLLKQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 267
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

BLAST of CmoCh19G009320 vs. NCBI nr
Match: KAG6572281.1 (Endoribonuclease Dicer-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 529.3 bits (1362), Expect = 2.0e-146
Identity = 263/266 (98.87%), Postives = 263/266 (98.87%), Query Frame = 0

Query: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEG GD GDLLKQEGRGGESTAASLDGMEAIL YEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGRGDRGDLLKQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 267
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

BLAST of CmoCh19G009320 vs. NCBI nr
Match: XP_023554575.1 (ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528.9 bits (1361), Expect = 2.6e-146
Identity = 262/266 (98.50%), Postives = 264/266 (99.25%), Query Frame = 0

Query: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDG DLL+QEGRGGESTAASLDGMEAIL YEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 267
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

BLAST of CmoCh19G009320 vs. NCBI nr
Match: XP_022969213.1 (ribonuclease 3-like protein 3 [Cucurbita maxima])

HSP 1 Score: 526.2 bits (1354), Expect = 1.7e-145
Identity = 261/266 (98.12%), Postives = 263/266 (98.87%), Query Frame = 0

Query: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDG DLLKQEGR GESTAASLDGMEAIL YEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGVDLLKQEGRVGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNG+IKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGMIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 267
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

BLAST of CmoCh19G009320 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 247.7 bits (631), Expect = 1.1e-65
Identity = 132/253 (52.17%), Postives = 167/253 (66.01%), Query Frame = 0

Query: 6   DGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYV 65
           DG D  K  G         ++ +E IL Y+F+DK LL +AFTDASY  +   S+E LE +
Sbjct: 10  DGKDNTKTIGSADPDQLMEIESLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELL 69

Query: 66  GDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQ 125
           GDS+LN  I  +     P  +PG LT+LRA NVDTEKLARVA+ H  + YLRH K  L++
Sbjct: 70  GDSILNMGIIYDFIKLYPKEAPGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEE 129

Query: 126 KIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLIT 185
           +I EF + +E+YPLHSNGL+K PK+LADIVES IGA+F D NS +TVW + K L+EP+I 
Sbjct: 130 QILEFVEAMEKYPLHSNGLLKVPKVLADIVESTIGAIFMDCNSTETVWKVIKPLLEPIIH 189

Query: 186 LKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYG-KKLIAQNRA 245
           L  MK HPMTEL EMCQKRNLK    D WEE +     I+ + VG G +  KK  A+N A
Sbjct: 190 LDKMKSHPMTELNEMCQKRNLKLTSKDTWEENQTYCFHIEDKFVGCGHHPVKKETARNFA 249

Query: 246 AKDALDNFHTILG 258
           AK+A+DNF    G
Sbjct: 250 AKNAIDNFAKFFG 262

BLAST of CmoCh19G009320 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 172.9 bits (437), Expect = 3.4e-43
Identity = 104/241 (43.15%), Postives = 145/241 (60.17%), Query Frame = 0

Query: 19   ESTAASLD--GMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITR 78
            ES   S+D  G+E  LKYEF++K LL EA T AS      S ++RLE+VGD+VL+ LITR
Sbjct: 1551 ESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITR 1610

Query: 79   EQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIE- 138
              +F+  +L PG LT LRAA V+ E  ARVA+ H  H YLRH  + L+++I EF K ++ 
Sbjct: 1611 HLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQT 1670

Query: 139  ---EYPLHSNGL--IKAPKILADIVESCIGAVFTDSNSIDT-VWTIFKGLMEPLITLKTM 198
               +   +S GL   KAPK+L DIVES  GA+F DS    T  W +F+ L++P++T +T+
Sbjct: 1671 ESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETL 1730

Query: 199  KRHPMTELTEMCQKRNLKWEF-VDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDA 250
              HP+ EL E CQ++    E+          + V ID   VG     +K +AQ  AA++A
Sbjct: 1731 PMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNA 1790

BLAST of CmoCh19G009320 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 172.9 bits (437), Expect = 3.4e-43
Identity = 104/241 (43.15%), Postives = 145/241 (60.17%), Query Frame = 0

Query: 19   ESTAASLD--GMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITR 78
            ES   S+D  G+E  LKYEF++K LL EA T AS      S ++RLE+VGD+VL+ LITR
Sbjct: 1552 ESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITR 1611

Query: 79   EQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIE- 138
              +F+  +L PG LT LRAA V+ E  ARVA+ H  H YLRH  + L+++I EF K ++ 
Sbjct: 1612 HLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQT 1671

Query: 139  ---EYPLHSNGL--IKAPKILADIVESCIGAVFTDSNSIDT-VWTIFKGLMEPLITLKTM 198
               +   +S GL   KAPK+L DIVES  GA+F DS    T  W +F+ L++P++T +T+
Sbjct: 1672 ESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETL 1731

Query: 199  KRHPMTELTEMCQKRNLKWEF-VDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDA 250
              HP+ EL E CQ++    E+          + V ID   VG     +K +AQ  AA++A
Sbjct: 1732 PMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNA 1791

BLAST of CmoCh19G009320 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 168.3 bits (425), Expect = 8.3e-42
Identity = 94/237 (39.66%), Postives = 149/237 (62.87%), Query Frame = 0

Query: 20  STAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQY 79
           S   S++ +E IL Y+F +K LL+EA T  S +  +  S+ERLE++GDS +   I+   Y
Sbjct: 55  SEMESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAISNYLY 114

Query: 80  FSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNI---EE 139
            + P+L P  L+ LRAANV TEKLARV++ HG + +LR N   LD+K++EFS+ +   ++
Sbjct: 115 LTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDD 174

Query: 140 YPLHSNGLIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKR--HP 199
             +   GL+KAPK+LAD+ ES  GAV+ D N  +  +W IF+GL+EP++TL  +++   P
Sbjct: 175 LSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQP 234

Query: 200 MTELTEMCQKRNLKWEFVDLWEERKEIHVL-IDKQLVGKGSYGKKLIAQNRAAKDAL 250
           ++ L ++C K   + +  +  +    I V+ +D +L+  G    K IA+  AAK+AL
Sbjct: 235 VSMLFKLCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEAL 289

BLAST of CmoCh19G009320 vs. TAIR 10
Match: AT3G03300.1 (dicer-like 2 )

HSP 1 Score: 132.9 bits (333), Expect = 3.8e-31
Identity = 78/189 (41.27%), Postives = 112/189 (59.26%), Query Frame = 0

Query: 28   MEAILKYEFRDKRLLEEAFTDASY-SPENCSSFERLEYVGDSVLNFLITREQYFSNPNLS 87
            ME++L Y F DK LL EA T  SY  PE    ++RLE++GDSVL++LIT+  Y   P LS
Sbjct: 1152 MESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLS 1211

Query: 88   PGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHS----N 147
            PG LT +R+A+V+ E  A VA+    H+++ +    L + I       E+  L S     
Sbjct: 1212 PGLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWE 1271

Query: 148  GLIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMC 207
              I  PK+L D++ES  GA+F DS  + + V+   K L+  +IT +T+K HP+ ELTE+C
Sbjct: 1272 SDISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELC 1331

Query: 208  QKRNLKWEF 211
            Q    KW+F
Sbjct: 1332 Q----KWQF 1336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q69KJ07.2e-5950.42Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0... [more]
Q9SP324.7e-4243.15Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV... [more]
Q9LTQ01.2e-4039.66Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1[more]
Q6ATG62.0e-4040.26Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0... [more]
Q8LMR26.4e-3941.03Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL... [more]
Match NameE-valueIdentityDescription
A0A6J1GKQ43.5e-149100.00ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4... [more]
A0A6J1I0C18.1e-14698.12ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 S... [more]
A0A0A0K1J81.3e-11682.51RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 P... [more]
A0A6J1C3752.5e-11585.60ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=... [more]
A0A1Q3BNT87.0e-8166.67Ribonuclease_3 domain-containing protein (Fragment) OS=Cephalotus follicularis O... [more]
Match NameE-valueIdentityDescription
XP_022952611.17.3e-149100.00ribonuclease 3-like protein 3 [Cucurbita moschata][more]
KAG7011897.12.3e-14799.25Ribonuclease 3-like protein 3, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
KAG6572281.12.0e-14698.87Endoribonuclease Dicer-like 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023554575.12.6e-14698.50ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo][more]
XP_022969213.11.7e-14598.12ribonuclease 3-like protein 3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G15417.11.1e-6552.17RNAse II-like 1 [more]
AT1G01040.13.4e-4343.15dicer-like 1 [more]
AT1G01040.23.4e-4343.15dicer-like 1 [more]
AT3G20420.18.3e-4239.66RNAse THREE-like protein 2 [more]
AT3G03300.13.8e-3141.27dicer-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000999Ribonuclease III domainSMARTSM00535riboneu5coord: 40..189
e-value: 3.9E-33
score: 126.1
IPR000999Ribonuclease III domainPFAMPF00636Ribonuclease_3coord: 60..166
e-value: 5.2E-22
score: 78.5
IPR000999Ribonuclease III domainPROSITEPS00517RNASE_3_1coord: 60..68
IPR000999Ribonuclease III domainPROSITEPS50142RNASE_3_2coord: 25..167
score: 33.284996
IPR000999Ribonuclease III domainCDDcd00593RIBOccoord: 40..185
e-value: 1.59497E-39
score: 132.351
NoneNo IPR availableGENE3D3.30.160.20coord: 193..261
e-value: 4.4E-7
score: 31.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR14950DICER-RELATEDcoord: 22..257
NoneNo IPR availablePANTHERPTHR14950:SF54RNASE II-LIKE 1coord: 22..257
NoneNo IPR availableSUPERFAMILY54768dsRNA-binding domain-likecoord: 149..252
IPR036389Ribonuclease III, endonuclease domain superfamilyGENE3D1.10.1520.10Ribonuclease III domaincoord: 20..192
e-value: 3.0E-48
score: 166.0
IPR036389Ribonuclease III, endonuclease domain superfamilySUPERFAMILY69065RNase III domain-likecoord: 25..186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh19G009320.1CmoCh19G009320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030422 production of siRNA involved in RNA interference
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0006396 RNA processing
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0004525 ribonuclease III activity
molecular_function GO:0003723 RNA binding