CmoCh19G009180 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh19G009180
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionO-methyltransferase
LocationCmo_Chr19: 8557587 .. 8559004 (+)
RNA-Seq ExpressionCmoCh19G009180
SyntenyCmoCh19G009180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGCAGTGGAGGGCGACGAGCTCCTGGAAGCTCAATCTCACATATGGAATCACATCTTGGCGATTAACAAGTCCATGGCTCTCAAATATGCGGTTCAAGTTGGCATACCAGACATCATCCACCGCTATGGCCCCAACCCCATGCCGCTCTCTAAACTTGTCTCCGCCCTACAGCTCCACCCGCAAAACACCCCTCACATGCATCGCTTAATGCGAGTATTGGTTCACTCTGGCTTCTTTCGAACGCAAAAACTGAGTGAACCAAACGGTGAAGAAGACGAGGGGTATGTGCTCACCAATTCATCTCGTCTTCTTGTTACAACCAACCCTTTGACTCTAGCTCCTTTTGTGTTTTGTGTTCTTGAGTCGGCCTTCTTCCAATCATGGCGCTCCCTCCCTGCCTGGCTCCAAACTGAGGACCGAACACCCTTTGACACAGCCAATGGAGTCTCGTTTTGGGAGTGTCTTGCGAATGACTCGACGGATGATGGCGTCATTTTTAACAAGTCCATGGCGAGTGATGCCAAGTTTGTGATGCGTATTTTGTTGGAGAAATATAAAGATGTGTTCGATGGGTTTGGGTCGTTGGTTGATGTGGGAGGTGGGACTGGAAATGTGGTCAAAGCCATTGCTCAAGCCTTTCCTAAAATGGAATGCACTGTGTTTGATCTCCCACAGGTTGTGGGTGATTTGAAAGGCGAAGCCAACTTCAAATATGTTCAAGGGGACATGTTTCATGCCATTCCTCCAGCTGATGCAATTCTATTGAAGGTAAAAACTTCATTTCCTTTTTTATATATACGTACCGATACCTTATTCTTATTAGATGAACACGACTCTCCACAATGATACCGATACCTTATTTTTATTAGATGAACACGACTCTCCACAATGGTACCGATACCTTATTCTTATTAGATCAACACGACTCTCCACAATGGTATGATATTGTCCACTTTGAGCATGAGCTCTCGTGGCTCTATTTTGAGGTTCCAAAAGGTCTCACACCAATGGAGATAGTATTCATTGATTATAAACTCATGATCATTTTCTATATTAGCCGATGTGGGACTTTTATCATCTAATAATTCTAATTTGTGAACGTCTGAAAATAGTGGATACTACATGATTGGAACGACGAAGAATGTGTGAAGATTTTGAAGAACTGCAAAGAAGCAATTACGGGCAATAAAAAGAATGGGAAAGTGATAGTAATTGATATGGTGGTAGGTAACGACACAAGAGATGAGTGTACTGAAACTCAACTGTTTTTCGATATGATGATGATGCTGATGCTTGGTGGAAAAGAGAGAGATGAAAAAGAATGGGCTCAGCTTATCAAAGAAGCTGGATATAGTGGCTACAAGATATTTCCAATTCTGGGTTTGAGGTCTCTTGTCGAAATTTATCCTTGA

mRNA sequence

ATGGGAGCAGTGGAGGGCGACGAGCTCCTGGAAGCTCAATCTCACATATGGAATCACATCTTGGCGATTAACAAGTCCATGGCTCTCAAATATGCGGTTCAAGTTGGCATACCAGACATCATCCACCGCTATGGCCCCAACCCCATGCCGCTCTCTAAACTTGTCTCCGCCCTACAGCTCCACCCGCAAAACACCCCTCACATGCATCGCTTAATGCGAGTATTGGTTCACTCTGGCTTCTTTCGAACGCAAAAACTGAGTGAACCAAACGGTGAAGAAGACGAGGGGTATGTGCTCACCAATTCATCTCGTCTTCTTGTTACAACCAACCCTTTGACTCTAGCTCCTTTTGTGTTTTGTGTTCTTGAGTCGGCCTTCTTCCAATCATGGCGCTCCCTCCCTGCCTGGCTCCAAACTGAGGACCGAACACCCTTTGACACAGCCAATGGAGTCTCGTTTTGGGAGTGTCTTGCGAATGACTCGACGGATGATGGCGTCATTTTTAACAAGTCCATGGCGAGTGATGCCAAGTTTGTGATGCGTATTTTGTTGGAGAAATATAAAGATGTGTTCGATGGGTTTGGGTCGTTGGTTGATGTGGGAGGTGGGACTGGAAATGTGGTCAAAGCCATTGCTCAAGCCTTTCCTAAAATGGAATGCACTGTGTTTGATCTCCCACAGGTTGTGGGTGATTTGAAAGGCGAAGCCAACTTCAAATATGTTCAAGGGGACATGTTTCATGCCATTCCTCCAGCTGATGCAATTCTATTGAAGTGGATACTACATGATTGGAACGACGAAGAATGTGTGAAGATTTTGAAGAACTGCAAAGAAGCAATTACGGGCAATAAAAAGAATGGGAAAGTGATAGTAATTGATATGGTGGTAGGTAACGACACAAGAGATGAGTGTACTGAAACTCAACTGTTTTTCGATATGATGATGATGCTGATGCTTGGTGGAAAAGAGAGAGATGAAAAAGAATGGGCTCAGCTTATCAAAGAAGCTGGATATAGTGGCTACAAGATATTTCCAATTCTGGGTTTGAGGTCTCTTGTCGAAATTTATCCTTGA

Coding sequence (CDS)

ATGGGAGCAGTGGAGGGCGACGAGCTCCTGGAAGCTCAATCTCACATATGGAATCACATCTTGGCGATTAACAAGTCCATGGCTCTCAAATATGCGGTTCAAGTTGGCATACCAGACATCATCCACCGCTATGGCCCCAACCCCATGCCGCTCTCTAAACTTGTCTCCGCCCTACAGCTCCACCCGCAAAACACCCCTCACATGCATCGCTTAATGCGAGTATTGGTTCACTCTGGCTTCTTTCGAACGCAAAAACTGAGTGAACCAAACGGTGAAGAAGACGAGGGGTATGTGCTCACCAATTCATCTCGTCTTCTTGTTACAACCAACCCTTTGACTCTAGCTCCTTTTGTGTTTTGTGTTCTTGAGTCGGCCTTCTTCCAATCATGGCGCTCCCTCCCTGCCTGGCTCCAAACTGAGGACCGAACACCCTTTGACACAGCCAATGGAGTCTCGTTTTGGGAGTGTCTTGCGAATGACTCGACGGATGATGGCGTCATTTTTAACAAGTCCATGGCGAGTGATGCCAAGTTTGTGATGCGTATTTTGTTGGAGAAATATAAAGATGTGTTCGATGGGTTTGGGTCGTTGGTTGATGTGGGAGGTGGGACTGGAAATGTGGTCAAAGCCATTGCTCAAGCCTTTCCTAAAATGGAATGCACTGTGTTTGATCTCCCACAGGTTGTGGGTGATTTGAAAGGCGAAGCCAACTTCAAATATGTTCAAGGGGACATGTTTCATGCCATTCCTCCAGCTGATGCAATTCTATTGAAGTGGATACTACATGATTGGAACGACGAAGAATGTGTGAAGATTTTGAAGAACTGCAAAGAAGCAATTACGGGCAATAAAAAGAATGGGAAAGTGATAGTAATTGATATGGTGGTAGGTAACGACACAAGAGATGAGTGTACTGAAACTCAACTGTTTTTCGATATGATGATGATGCTGATGCTTGGTGGAAAAGAGAGAGATGAAAAAGAATGGGCTCAGCTTATCAAAGAAGCTGGATATAGTGGCTACAAGATATTTCCAATTCTGGGTTTGAGGTCTCTTGTCGAAATTTATCCTTGA

Protein sequence

MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQLHPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFCVLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVMRILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKYVQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDTRDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP
Homology
BLAST of CmoCh19G009180 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.5e-109
Identity = 196/355 (55.21%), Postives = 257/355 (72.39%), Query Frame = 0

Query: 8   ELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQLHPQNTPH 67
           E+L AQ+HIWN I     SM+LK A+Q+GIPDII+ +G  PM +S+L  AL ++ + +P 
Sbjct: 25  EILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHG-KPMTISQLTLALPINRKKSPC 84

Query: 68  MHRLMRVLVHSGFFRTQK--LSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFCVLESA 127
           ++RLMR+L+HSGFF  QK  + E  G E+EGYV+T++S+LL+  NP+++ PF+  +L+  
Sbjct: 85  VYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLDPV 144

Query: 128 FFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVMRILLE 187
             + W  L  W Q  D TPFDTANG++FW+   +        FN +MASDA+ V  +++E
Sbjct: 145 MTKPWDFLSNWFQNGDPTPFDTANGMAFWD-YGSHEPKLARFFNDAMASDARLVTSVVIE 204

Query: 188 KYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKYVQGDM 247
           K K VF+G  SLVDVGGGTG V  +IA AFP ++CTVFDLP VV DL+G  N  +V GDM
Sbjct: 205 KCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGGNNLNFVGGDM 264

Query: 248 FHAIPPADAILLKWILHDWNDEECVKILKNCKEAIT-GNKKNGKVIVIDMVVGN--DTRD 307
           F  +P  + +LLKWILHDWNDEE VKILK CKEAI+  NKK GKVI+IDM V N  D  D
Sbjct: 265 FVDVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDMKVENEKDEDD 324

Query: 308 ECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           E  ETQLFFDM+MM ++ G+ER+EKEWA+L K+AG+S YKI PILGLRSL+E+YP
Sbjct: 325 ESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGLRSLIEVYP 377

BLAST of CmoCh19G009180 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 389.8 bits (1000), Expect = 3.3e-107
Identity = 192/351 (54.70%), Postives = 251/351 (71.51%), Query Frame = 0

Query: 8   ELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQLHPQNTPH 67
           ELL AQ+H+WNHI    KSM+LK A+Q+GIPDIIH +G  PM L +LV+ L +HP+ +  
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPELVAKLPVHPKRSQC 70

Query: 68  MHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFCVLESAFF 127
           ++RLMR+LVHSGF   Q++ +  G+E+EGYVLT++SRLL+  + L++ P V  +L+    
Sbjct: 71  VYRLMRILVHSGFLAAQRVQQ--GKEEEGYVLTDASRLLLMDDSLSIRPLVLAMLDPILT 130

Query: 128 QSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVMRILLEKY 187
           + W  L AW Q +D TPF TA   SFW+   ++   +   FN++MASDA+ +  +LL++ 
Sbjct: 131 KPWHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNN-SFNEAMASDARLLTSVLLKEG 190

Query: 188 KDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKYVQGDMFH 247
           + VF G  SLVDVGGGTG V KAIA AFP + CTV DL  VV  L+G  N  Y  GDMF 
Sbjct: 191 QGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSKNLNYFAGDMFE 250

Query: 248 AIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDTRD-ECTE 307
           AIPPADAILLKWILHDW++EECVKILK C+EAI   +  GKVI+IDM++  +  D + TE
Sbjct: 251 AIPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQGDYKSTE 310

Query: 308 TQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           TQLFFDM MM+   G+ERDE EW +L  +AG+S YKI PILGLRSL+E+YP
Sbjct: 311 TQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

BLAST of CmoCh19G009180 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.4e-97
Identity = 180/364 (49.45%), Postives = 248/364 (68.13%), Query Frame = 0

Query: 2   GAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQLH 61
           G     ELLEAQ+H+WNHI +   SM+LK A+Q+GIPD IH++G NP+ LS+L  AL ++
Sbjct: 6   GISSKQELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHG-NPITLSQLADALNIN 65

Query: 62  PQNTPHMHRLMRVLVHSGFFRTQKL-----SEPNGEEDEGYVLTNSSRLLVTTNPLTLAP 121
              +  + RLMR+LVHSGFF   K+      E   EE++ Y LT +SRLL+ + PL++AP
Sbjct: 66  KAKSHGLFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAP 125

Query: 122 FVFCVLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWE-CLANDSTDDGVIFNKSMASD 181
           F   + +  + ++W  L  W + +    FDT  G++F E  +A+D  +  V+FN++MA D
Sbjct: 126 FALAMSDPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLN--VLFNEAMACD 185

Query: 182 AKFVMRILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGE 241
           A FV  IL  + +++FDG  S+VDVGGGTG   K IA AFP MECTV DLP VVG LKG 
Sbjct: 186 AGFVNSILTTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGS 245

Query: 242 ANFKYVQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMV 301
            N  +V GDMF  IP ADAI +K+ILHDWNDEECVKILK CKEAI+ +  + + I++  +
Sbjct: 246 ENLSFVSGDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNSCRKIILVEI 305

Query: 302 VGNDTRD--ECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLV 358
           V  D ++  E TET+LFFDM M+ ++ GKER EKEW +L  +AG++ YKI  +LGLRS++
Sbjct: 306 VMEDEKETHEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITRVLGLRSVI 365

BLAST of CmoCh19G009180 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 6.4e-95
Identity = 167/355 (47.04%), Postives = 242/355 (68.17%), Query Frame = 0

Query: 6   GDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQLHPQNT 65
           G+ELL AQ+HI+NHI     SMALK AV++ IP+I+H + P  + LS+LV ALQ+    +
Sbjct: 21  GEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTLSELVQALQIPQAKS 80

Query: 66  PHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFCVLESA 125
             ++RL+R+LVHSGFF   K+      ++EGY+ T SS+LL+  +P++++P +  ++   
Sbjct: 81  ACLYRLLRILVHSGFFAITKIQSEG--DEEGYLPTLSSKLLLKNHPMSMSPCLLGLVNPT 140

Query: 126 FFQSWRSLPAWL-QTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVMRILL 185
                     W  +++D TPF+  +G S W+    ++     IFN++M  + +  M ++L
Sbjct: 141 MVAPMHFFSDWFKRSDDMTPFEATHGASLWKYF-GETPHMAEIFNEAMGCETRLAMSVVL 200

Query: 186 EKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKYVQGD 245
           ++ K   +G  SLVDVGGGTGNV +AIA+AFP ++CTV DLPQVVG+LKG  N ++V GD
Sbjct: 201 KECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGSNNLEFVSGD 260

Query: 246 MFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDTRD-- 305
           MF  IPPAD + LKWILHDWNDEEC+KILK CKEAI   ++ GK+I+IDMVV +  +   
Sbjct: 261 MFQFIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDMVVNDHNKGSY 320

Query: 306 ECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           E TETQLF+D+ +M +L G ER E EW +L   AG++ Y I P+LGL+S++E++P
Sbjct: 321 ESTETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLGLKSIIEVFP 372

BLAST of CmoCh19G009180 vs. ExPASy Swiss-Prot
Match: Q84KK4 (Isoflavone 4'-O-methyltransferase OS=Lotus japonicus OX=34305 GN=HI4'OMT PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 8.7e-92
Identity = 171/358 (47.77%), Postives = 239/358 (66.76%), Query Frame = 0

Query: 5   EGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQLHPQN 64
           E  EL +AQ H++ H+     SMALK A+++GI D+IH +G  P+ L +L +AL L P  
Sbjct: 10  EDTELSQAQIHLYKHVYNFVSSMALKSAMELGIADVIHSHG-KPITLPELATALNLRPSK 69

Query: 65  TPHMHRLMRVLVHSGFFRTQKLSEPNG-EEDEGYVLTNSSRLLVTTNPLTLAPFVFCVLE 124
              +HR +R+L H+GFF    +S   G EE+  Y LT  S+LLV +N   LAP V   L 
Sbjct: 70  IGVLHRFLRLLTHNGFFAKTTVSRGEGAEEETAYGLTPPSKLLVKSNSTCLAPIVKGALH 129

Query: 125 SAFFQSWRSLPAWL--QTEDRTPFDTANGVSFWECLANDSTDDGV-IFNKSMASDAKFVM 184
            +    WRS   W     E+ T F++A G SFWE L  ++  D + +F ++MA+D+  + 
Sbjct: 130 PSSLDMWRSSKKWFLEDNEELTLFESATGESFWEFLNKETESDTLSMFQEAMAADS-HMF 189

Query: 185 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 244
           ++ L++ K VF+G GSLVDV GG G V K I +AFP ++CTVFD PQVV +L G+ N  +
Sbjct: 190 KLALKECKHVFEGLGSLVDVAGGRGGVTKLIREAFPHVKCTVFDQPQVVANLTGDENLNF 249

Query: 245 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 304
           V GDMF ++PPADA+LLKW+LHDWNDE  +KILKNCKEAI+G  K GKVI+ID+ +   +
Sbjct: 250 VGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKEGKVIIIDISIDETS 309

Query: 305 RD-ECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
            D E TE +L +D++M+ M  GKER++KEW +LI +AG+S YKI PI G +SL+E++P
Sbjct: 310 DDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYDAGFSSYKITPICGFKSLIEVFP 365

BLAST of CmoCh19G009180 vs. ExPASy TrEMBL
Match: A0A6J1GLH7 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455049 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 1.8e-209
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 60
           MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL
Sbjct: 8   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 67

Query: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 120
           HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC
Sbjct: 68  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 127

Query: 121 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM 180
           VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM
Sbjct: 128 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM 187

Query: 181 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 240
           RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY
Sbjct: 188 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 247

Query: 241 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 300
           VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT
Sbjct: 248 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 307

Query: 301 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP
Sbjct: 308 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 364

BLAST of CmoCh19G009180 vs. ExPASy TrEMBL
Match: A0A6J1GLE5 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455048 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 9.1e-193
Identity = 328/357 (91.88%), Postives = 340/357 (95.24%), Query Frame = 0

Query: 1   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 60
           MG VEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL
Sbjct: 8   MGEVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 67

Query: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 120
           HPQ TPHMHRLMRVLVHSGFFRTQKLSE NGEEDEGYVLTNSSRLLVTTNPLTLAP+VF 
Sbjct: 68  HPQKTPHMHRLMRVLVHSGFFRTQKLSEQNGEEDEGYVLTNSSRLLVTTNPLTLAPYVFF 127

Query: 121 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM 180
           V ES  FQSWRSLP WLQTEDR+PFDTANGVSFWECL NDSTDDG IFNKSMASDAKFV+
Sbjct: 128 VFESPLFQSWRSLPTWLQTEDRSPFDTANGVSFWECLGNDSTDDGDIFNKSMASDAKFVI 187

Query: 181 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 240
           RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGE NFKY
Sbjct: 188 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGECNFKY 247

Query: 241 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 300
           V+GDMFHAIPPADAILLKWI+HDWNDEECVKILKNCKEAI  NKKNGKVIVIDMVVGN+ 
Sbjct: 248 VEGDMFHAIPPADAILLKWIMHDWNDEECVKILKNCKEAIASNKKNGKVIVIDMVVGNNK 307

Query: 301 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           RD+  ETQL FDM+MMLMLGGKERDEK+WAQLIK+AG+SGYKIFPILGLRSL+EIYP
Sbjct: 308 RDDLIETQLSFDMVMMLMLGGKERDEKQWAQLIKDAGFSGYKIFPILGLRSLIEIYP 364

BLAST of CmoCh19G009180 vs. ExPASy TrEMBL
Match: A0A5A7UZZ2 (Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G00160 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 2.1e-144
Identity = 257/365 (70.41%), Postives = 298/365 (81.64%), Query Frame = 0

Query: 1   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 60
           M  +E  +LLEAQSH+WN I    KSMALK AVQ+GIPD I R+GPNPMPLS+LVSAL+L
Sbjct: 1   MEVLEAKDLLEAQSHVWNQIFIFTKSMALKCAVQLGIPDAIDRHGPNPMPLSRLVSALEL 60

Query: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNG------EEDEGYVLTNSSRLLVTTNPLTL 120
             + TP+++RLMRVLVHSGFF TQKLSE N       EED GYVLTNSSRLLV TNPLTL
Sbjct: 61  PLEKTPYIYRLMRVLVHSGFFLTQKLSEQNDEDQEEEEEDHGYVLTNSSRLLVKTNPLTL 120

Query: 121 APFVFCVLESAFFQSWRSLPAWLQTEDRT-PFDTANGVSFWECL-ANDSTDDGVIFNKSM 180
           APF+F  L      SW SLPAWLQ+ED+T PF++A+GVSFWE + +N S++   +F+ S+
Sbjct: 121 APFLFSTLTPPLLDSWSSLPAWLQSEDQTPPFESAHGVSFWEYMGSNKSSEGAAVFDASL 180

Query: 181 ASDAKFVMRILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDL 240
           ASDAKFV+ +L+EK K VFDG GSLVDVGGGTGNV K IAQAFP+MECTVFDLP+VV DL
Sbjct: 181 ASDAKFVVSVLMEKCKGVFDGVGSLVDVGGGTGNVTKCIAQAFPEMECTVFDLPKVVADL 240

Query: 241 KGEANFKYVQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVI 300
           K E N K+V GDMF +IPPADA+LLKW+LHDW+DEECVKILK CKEAI  N+K GKV+VI
Sbjct: 241 KAEGNLKFVGGDMFKSIPPADALLLKWVLHDWSDEECVKILKKCKEAINRNEKKGKVMVI 300

Query: 301 DMVVGNDTRDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSL 358
           DMVVGN+  D   ETQLFFDMM+MLM+GGKERDEKEWA LI EAG+SGY IFPILGLRSL
Sbjct: 301 DMVVGNNKTDASIETQLFFDMMVMLMVGGKERDEKEWAHLIIEAGFSGYNIFPILGLRSL 360

BLAST of CmoCh19G009180 vs. ExPASy TrEMBL
Match: A0A5A7UTA4 (Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2933G00010 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.7e-144
Identity = 257/366 (70.22%), Postives = 298/366 (81.42%), Query Frame = 0

Query: 1   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 60
           M  +E  +LLEAQSH+WN I    KSMALK AVQ+GIPD I R+GPNPMPLS+LVSAL+L
Sbjct: 1   MEVLEAKDLLEAQSHVWNQIFIFTKSMALKCAVQLGIPDAIDRHGPNPMPLSRLVSALEL 60

Query: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNG-------EEDEGYVLTNSSRLLVTTNPLT 120
             + TP+++RLMRVLVHSGFF TQKLSE N        EED GYVLTNSSRLLV TNPLT
Sbjct: 61  PLEKTPYIYRLMRVLVHSGFFLTQKLSEQNDEDQEEEEEEDHGYVLTNSSRLLVKTNPLT 120

Query: 121 LAPFVFCVLESAFFQSWRSLPAWLQTEDRT-PFDTANGVSFWECL-ANDSTDDGVIFNKS 180
           LAPF+F  L      SW SLPAWLQ+ED+T PF++A+GVSFWE + +N S++   +F+ S
Sbjct: 121 LAPFLFSTLTPPLLDSWSSLPAWLQSEDQTPPFESAHGVSFWEYMGSNKSSEGAAVFDAS 180

Query: 181 MASDAKFVMRILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGD 240
           +ASDAKFV+ +L+EK K VFDG GSLVDVGGGTGNV K IAQAFP+MECTVFDLP+VV D
Sbjct: 181 LASDAKFVVSVLMEKCKGVFDGVGSLVDVGGGTGNVTKCIAQAFPEMECTVFDLPKVVAD 240

Query: 241 LKGEANFKYVQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIV 300
           LK E N K+V GDMF +IPPADA+LLKW+LHDW+DEECVKILK CKEAI  N+K GKV+V
Sbjct: 241 LKAEGNLKFVGGDMFKSIPPADALLLKWVLHDWSDEECVKILKKCKEAINRNEKKGKVMV 300

Query: 301 IDMVVGNDTRDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRS 358
           IDMVVGN+  D   ETQLFFDMM+MLM+GGKERDEKEWA LI EAG+SGY IFPILGLRS
Sbjct: 301 IDMVVGNNKTDASIETQLFFDMMVMLMVGGKERDEKEWAHLIIEAGFSGYNIFPILGLRS 360

BLAST of CmoCh19G009180 vs. ExPASy TrEMBL
Match: A0A6J1C1F3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE=3 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 4.9e-138
Identity = 245/355 (69.01%), Postives = 285/355 (80.28%), Query Frame = 0

Query: 3   AVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQLHP 62
           AV   ELLEAQ+HIWNHI     SMALK AVQ+GIPD IHR+GPNPMPLS+LVSALQL P
Sbjct: 10  AVPSHELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGPNPMPLSQLVSALQLSP 69

Query: 63  QNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFCVL 122
           Q T +++RLMR+L HS FF T K+   NGEE E YVLTNSSRLL+  NP  + PF+  +L
Sbjct: 70  QKTQYIYRLMRLLTHSSFFVTHKMMTKNGEE-EAYVLTNSSRLLLNDNPSAVTPFLLSML 129

Query: 123 ESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVMRI 182
           E A  + W+ L AWLQ+EDRTPF T +G+ FWE   ++   DG +FN +MASDA+ VM I
Sbjct: 130 EPALVEPWQLLAAWLQSEDRTPFHTTHGMPFWE-FFSEKPKDGDVFNVAMASDARLVMSI 189

Query: 183 LLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKYVQ 242
           LL+KYK VF+G GSLVDVGGGTG V KAIA+AFP++EC VFDL QVVG+LKGE N KYV+
Sbjct: 190 LLDKYKGVFEGVGSLVDVGGGTGTVAKAIAKAFPQIECIVFDLHQVVGNLKGEDNLKYVE 249

Query: 243 GDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDTRD 302
           G+MF A+P ADA+LLKWILHDWNDEECV+ILK CKEAIT N K GK+IVIDMVVGN   D
Sbjct: 250 GNMFEAVPSADALLLKWILHDWNDEECVEILKKCKEAITNNGKKGKMIVIDMVVGNKQGD 309

Query: 303 ECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           +  ETQLFFDM+MM + GGKERDEKEWAQLIKEAG SGYKIFPI+GLRSL+EIYP
Sbjct: 310 DSIETQLFFDMLMMNLAGGKERDEKEWAQLIKEAGLSGYKIFPIMGLRSLIEIYP 362

BLAST of CmoCh19G009180 vs. NCBI nr
Match: XP_022952340.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 738.0 bits (1904), Expect = 3.8e-209
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 60
           MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL
Sbjct: 8   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 67

Query: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 120
           HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC
Sbjct: 68  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 127

Query: 121 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM 180
           VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM
Sbjct: 128 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM 187

Query: 181 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 240
           RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY
Sbjct: 188 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 247

Query: 241 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 300
           VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT
Sbjct: 248 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 307

Query: 301 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP
Sbjct: 308 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 364

BLAST of CmoCh19G009180 vs. NCBI nr
Match: XP_023554531.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724.5 bits (1869), Expect = 4.3e-205
Identity = 350/357 (98.04%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 60
           MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVS+LQL
Sbjct: 8   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSSLQL 67

Query: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 120
           HPQNTPHMHRLMRVLVHSGFFRTQKLSEPN EEDEGYVLTNSSRLLVTTNPLTLAPFVFC
Sbjct: 68  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNVEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 127

Query: 121 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM 180
           VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECL NDSTDDGVIFNKSMASDAKFVM
Sbjct: 128 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLGNDSTDDGVIFNKSMASDAKFVM 187

Query: 181 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 240
           RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGE NFKY
Sbjct: 188 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEGNFKY 247

Query: 241 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 300
           VQGDMFHAIP ADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT
Sbjct: 248 VQGDMFHAIPSADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 307

Query: 301 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           RDECTETQLFFDMMMMLMLGGKERDEK+WAQLIKEAG+SGYKIFPILGLRSLVEIYP
Sbjct: 308 RDECTETQLFFDMMMMLMLGGKERDEKQWAQLIKEAGFSGYKIFPILGLRSLVEIYP 364

BLAST of CmoCh19G009180 vs. NCBI nr
Match: KAG6572270.1 (Trans-resveratrol di-O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 721.1 bits (1860), Expect = 4.8e-204
Identity = 345/357 (96.64%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 60
           MG V+G+ELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLS+LVSALQL
Sbjct: 1   MGEVDGEELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSQLVSALQL 60

Query: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 120
           HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC
Sbjct: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 120

Query: 121 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM 180
           VLESAFFQSWRSLPAWLQT+DRTPFDTANGVSFWECL NDSTDDGVIFNKSMASDAKFVM
Sbjct: 121 VLESAFFQSWRSLPAWLQTQDRTPFDTANGVSFWECLGNDSTDDGVIFNKSMASDAKFVM 180

Query: 181 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 240
           RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVV DLKGE NFKY
Sbjct: 181 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVADLKGEGNFKY 240

Query: 241 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 300
           V+GDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT
Sbjct: 241 VEGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 300

Query: 301 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAG++GYKIFPILGLRSL+EIYP
Sbjct: 301 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGFNGYKIFPILGLRSLIEIYP 357

BLAST of CmoCh19G009180 vs. NCBI nr
Match: XP_022952339.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 682.6 bits (1760), Expect = 1.9e-192
Identity = 328/357 (91.88%), Postives = 340/357 (95.24%), Query Frame = 0

Query: 1   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 60
           MG VEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL
Sbjct: 8   MGEVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 67

Query: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 120
           HPQ TPHMHRLMRVLVHSGFFRTQKLSE NGEEDEGYVLTNSSRLLVTTNPLTLAP+VF 
Sbjct: 68  HPQKTPHMHRLMRVLVHSGFFRTQKLSEQNGEEDEGYVLTNSSRLLVTTNPLTLAPYVFF 127

Query: 121 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM 180
           V ES  FQSWRSLP WLQTEDR+PFDTANGVSFWECL NDSTDDG IFNKSMASDAKFV+
Sbjct: 128 VFESPLFQSWRSLPTWLQTEDRSPFDTANGVSFWECLGNDSTDDGDIFNKSMASDAKFVI 187

Query: 181 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 240
           RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGE NFKY
Sbjct: 188 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGECNFKY 247

Query: 241 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 300
           V+GDMFHAIPPADAILLKWI+HDWNDEECVKILKNCKEAI  NKKNGKVIVIDMVVGN+ 
Sbjct: 248 VEGDMFHAIPPADAILLKWIMHDWNDEECVKILKNCKEAIASNKKNGKVIVIDMVVGNNK 307

Query: 301 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           RD+  ETQL FDM+MMLMLGGKERDEK+WAQLIK+AG+SGYKIFPILGLRSL+EIYP
Sbjct: 308 RDDLIETQLSFDMVMMLMLGGKERDEKQWAQLIKDAGFSGYKIFPILGLRSLIEIYP 364

BLAST of CmoCh19G009180 vs. NCBI nr
Match: XP_023554527.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 681.4 bits (1757), Expect = 4.2e-192
Identity = 328/357 (91.88%), Postives = 340/357 (95.24%), Query Frame = 0

Query: 1   MGAVEGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 60
           MG V+GDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL
Sbjct: 8   MGEVDGDELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL 67

Query: 61  HPQNTPHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFC 120
           HPQ TPHMHRLMRVLVHSGFFRTQKLSE NGEEDEGYVLTNSSRLLVTTNPLTLAP+VF 
Sbjct: 68  HPQKTPHMHRLMRVLVHSGFFRTQKLSEQNGEEDEGYVLTNSSRLLVTTNPLTLAPYVFF 127

Query: 121 VLESAFFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVM 180
           V ES  FQSWRSLP WLQTEDR+PFDTANGVSFWECL NDSTDDG IFNKSMASDAKFV+
Sbjct: 128 VFESPLFQSWRSLPTWLQTEDRSPFDTANGVSFWECLGNDSTDDGDIFNKSMASDAKFVI 187

Query: 181 RILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKY 240
           RILLEKYKDVFDGFGSLVDVGGGTGNVVK+IAQAFPKMECTVFDLPQVV DLKGE NFKY
Sbjct: 188 RILLEKYKDVFDGFGSLVDVGGGTGNVVKSIAQAFPKMECTVFDLPQVVADLKGEGNFKY 247

Query: 241 VQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT 300
           VQGDMFHAIPPADAILLKWILHDWND+ECVKILKNCKEAI GNKKNGKVIVIDMVVGN+ 
Sbjct: 248 VQGDMFHAIPPADAILLKWILHDWNDDECVKILKNCKEAIMGNKKNGKVIVIDMVVGNNK 307

Query: 301 RDECTETQLFFDMMMMLMLGGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIYP 358
           RD+  ETQL FDM+MMLMLGGKERDEK+WAQLIKEAG+SGYKIFPILGLRSL+EIYP
Sbjct: 308 RDDFIETQLSFDMVMMLMLGGKERDEKQWAQLIKEAGFSGYKIFPILGLRSLIEIYP 364

BLAST of CmoCh19G009180 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 208.4 bits (529), Expect = 9.7e-54
Identity = 132/366 (36.07%), Postives = 198/366 (54.10%), Query Frame = 0

Query: 7   DELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRY-GPNPMPLSKLVSALQLHPQNT 66
           DE  +A   IW ++       A K A+ + IP+ I  +    P+ L++L SA+   P   
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPS-- 83

Query: 67  PHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTT--NPLTLAPFVFCVLE 126
            H+ R+MR LVH G F+  ++   +G    GYV T  SR L+ T  +  +LAPFV     
Sbjct: 84  -HLRRIMRFLVHQGIFK--EIPTKDGLA-TGYVNTPLSRRLMITRRDGKSLAPFVLFETT 143

Query: 127 SAFFQSWRSLPAWLQT----EDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFV 186
                 W  L + + +        PFD  +G   W   A D+     + N++MA DA+ V
Sbjct: 144 PEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWS-FAQDNPFLSDMINEAMACDARRV 203

Query: 187 MRILLEKYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFK 246
           +  +      +FDG  ++VDVGGGTG  +  + + FP ++   FDLP V+   +     +
Sbjct: 204 VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVE 263

Query: 247 YVQGDMFHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGN- 306
            V+GDMF +IP  DAI +KW+LHDW D++C+KILKNCKEA+  N   GKV++++ V+G  
Sbjct: 264 NVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPN--IGKVLIVESVIGEN 323

Query: 307 ------DTRDECTE-TQLFFDMMMMLMLG-GKERDEKEWAQLIKEAGYSGYKIFPILGLR 357
                 D RDE  E  +L  DM+MM     GKER  KEW  ++KEAG++ Y++  I  ++
Sbjct: 324 KKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQ 380

BLAST of CmoCh19G009180 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 181.4 bits (459), Expect = 1.3e-45
Identity = 113/360 (31.39%), Postives = 179/360 (49.72%), Query Frame = 0

Query: 7   DELLEAQSHIWNHILAINKSMALKYAVQVGIPDIIHRY-GPNPMPLSKLVSALQLHPQNT 66
           DE  +A   IW ++       A K A+ + IP+ I  +    P+ LS+L SA+   P   
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPS-- 69

Query: 67  PHMHRLMRVLVHSGFFRTQKLSEPNGEEDEGYVLTNSSRLLVTTNPLTLAPFVFCVLESA 126
            H+ R+MR LVH G F+     +       GY  T  SR ++ T                
Sbjct: 70  -HLRRIMRFLVHQGLFKEVPTKDGLA---TGYTNTPLSRRMMITK--------------- 129

Query: 127 FFQSWRSLPAWLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVMRILLE 186
                                  +G   W   A D+     + N++MA DA+ V+  +  
Sbjct: 130 ----------------------LHGKDLW-AFAQDNLCHSQLINEAMACDARRVVPRVAG 189

Query: 187 KYKDVFDGFGSLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKYVQGDM 246
             + +FDG  ++VDVGGGTG  +  + + FP ++   FDLP V+   +     + V+GDM
Sbjct: 190 ACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDM 249

Query: 247 FHAIPPADAILLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVG-------- 306
           F +IP +DA+++KW+LHDW D++C+KILKNCKEA+  N   GKV++++ V+G        
Sbjct: 250 FDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPN--IGKVLIVECVIGEKKNTMIA 309

Query: 307 NDTRDECTETQLFFDMMMMLMLG-GKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIY 357
            +  D+    +L  DM+MM+    GKER  KEW  ++ EAG++ Y++     ++SL+  Y
Sbjct: 310 EERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIAY 323

BLAST of CmoCh19G009180 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 169.9 bits (429), Expect = 3.8e-42
Identity = 116/339 (34.22%), Postives = 179/339 (52.80%), Query Frame = 0

Query: 29  LKYAVQVGIPDIIHRYGPNPMPLSKLVSALQLHPQNTPH----MHRLMRVLVHSGFFRTQ 88
           +K A ++ + +I+ +  P    LS +  A    P+N PH    + RL+R LV       +
Sbjct: 32  VKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKN-PHAPMMIDRLLRFLVAYSVCTCK 91

Query: 89  KLSEPNGEEDEGYVLTN-SSRLLVTTNPLTLAPFVFCVLESAFFQSWRSLPAWLQTEDRT 148
            + +  G E   Y L     +L+   +  ++AP+V      A    W  L   +Q    +
Sbjct: 92  LVKDEEGRESRAYGLGKVGKKLIKDEDGFSIAPYVLAGCTKAKGGVWSYLTEAIQEGGAS 151

Query: 149 PFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVMRILLEKYKDVFDGFGSLVDVGGG 208
            ++ AN    +E +  +  +   IFN+SM +    VM+ +LE Y   F+G    VDVGG 
Sbjct: 152 AWERANEALIFEYMKKNE-NLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGS 211

Query: 209 TGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKYVQGDMFHAIPPADAILLKWILHD 268
            G+ +  I   +P ++   FDLP +V +       +++ GDMF  IP  + IL+KWILHD
Sbjct: 212 LGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHD 271

Query: 269 WNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT--RDECTETQLFFDM-MMMLMLG 328
           WNDE+CV+ILKNCK+A+    + G++IVI+M+V  +    D  T+  L  D+ MM L  G
Sbjct: 272 WNDEKCVEILKNCKKAL---PETGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSG 331

Query: 329 GKERDEKEWAQLIKEAGYSGYKIFPILGLRS--LVEIYP 358
           GKER +KE+  L KEAG+   KI  I G  S  ++E+YP
Sbjct: 332 GKERTKKEFEDLAKEAGFKLPKI--IYGAYSYWIIELYP 362

BLAST of CmoCh19G009180 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 169.1 bits (427), Expect = 6.5e-42
Identity = 115/339 (33.92%), Postives = 180/339 (53.10%), Query Frame = 0

Query: 29  LKYAVQVGIPDIIHRYGPNPMPLSKLVSALQLHPQNTPH----MHRLMRVLVHSGFFRTQ 88
           +K A ++ + +I+ +  P    LS +  A    P+N PH    + RL+R LV       +
Sbjct: 32  VKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKN-PHAPMMIDRLLRFLVAYSVCTCK 91

Query: 89  KLSEPNGEEDEGYVLTN-SSRLLVTTNPLTLAPFVFCVLESAFFQSWRSLPAWLQTEDRT 148
            + +  G E   Y L     +L+   +  ++AP+V      A    W ++   +Q    +
Sbjct: 92  LVKDEEGRESRAYGLGKVGKKLIKDEDGFSIAPYVLAGCTKAKGGVWYNVQHAIQEGGAS 151

Query: 149 PFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVMRILLEKYKDVFDGFGSLVDVGGG 208
            ++ AN    +E +  +  +   IFN+SM +    VM+ +LE Y   F+G    VDVGG 
Sbjct: 152 AWERANEALIFEYMKKNE-NLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGS 211

Query: 209 TGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKYVQGDMFHAIPPADAILLKWILHD 268
            G+ +  I   +P ++   FDLP +V +       +++ GDMF  IP  + IL+KWILHD
Sbjct: 212 LGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHD 271

Query: 269 WNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDT--RDECTETQLFFDM-MMMLMLG 328
           WNDE+CV+ILKNCK+A+    + G++IVI+M+V  +    D  T+  L  D+ MM L  G
Sbjct: 272 WNDEKCVEILKNCKKAL---PETGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSG 331

Query: 329 GKERDEKEWAQLIKEAGYSGYKIFPILGLRS--LVEIYP 358
           GKER +KE+  L KEAG+   KI  I G  S  ++E+YP
Sbjct: 332 GKERTKKEFEDLAKEAGFKLPKI--IYGAYSYWIIELYP 362

BLAST of CmoCh19G009180 vs. TAIR 10
Match: AT5G53810.1 (O-methyltransferase family protein )

HSP 1 Score: 156.0 bits (393), Expect = 5.7e-38
Identity = 111/344 (32.27%), Postives = 172/344 (50.00%), Query Frame = 0

Query: 27  MALKYAVQVGIPDIIHRYGPNPMPLSKLVSALQL--HPQNTPH---MHRLMRVLVHSGFF 86
           M LK A+++G+ D I   G   + LS    AL+L   P N      + R++R LV     
Sbjct: 40  MVLKAALELGVIDTITTVGGGDLWLSPSEIALRLPTKPCNLEAPALLDRMLRFLVSHSVL 99

Query: 87  RTQKLSEPNGEE---DEGYVLTNSSRLLVTTN---PLTLAPFVFCVLESAFFQSWRSLPA 146
           + + + E NG+    +  Y      + L+  +     + A      L   F ++W  L  
Sbjct: 100 KCRTVIEENGQTGKVERVYAAEPVCKYLLNKSDDVSGSFASLFMLDLSDVFIKTWTHLED 159

Query: 147 WLQTEDRTPFDTANGVSFWECLANDSTDDGVIFNKSMASDAKFVMRILLEKYKDVFDGFG 206
            +  E R  F +A+G+  +E +  D    G +FN++M   +  V   +L K+ + F    
Sbjct: 160 VI-LEGRDAFSSAHGMKLFEYIQADER-FGKVFNRAMLESSTMVTEKVL-KFYEGFKDVK 219

Query: 207 SLVDVGGGTGNVVKAIAQAFPKMECTVFDLPQVVGDLKGEANFKYVQGDMFHAIPPADAI 266
           +LVDVGGG GN +  I   +P +    FDL  V+ +        +V GDMF  IP  DAI
Sbjct: 220 TLVDVGGGLGNTLGLITSKYPHLIGINFDLAPVLANAHSYPGVNHVAGDMFIKIPKGDAI 279

Query: 267 LLKWILHDWNDEECVKILKNCKEAITGNKKNGKVIVIDMVVGNDTR--DECTETQLFFDM 326
            +KWILHDW DE+CV ILKNC +++   ++NGK+I+++MV   + +  D C+      DM
Sbjct: 280 FMKWILHDWTDEQCVAILKNCWKSL---EENGKLIIVEMVTPVEAKSGDICSNIVFGMDM 339

Query: 327 MMMLML-GGKERDEKEWAQLIKEAGYSGYKIFPILGLRSLVEIY 357
            M+    GGKERD  E+  L   +G+S   I   +   S++EIY
Sbjct: 340 TMLTQCSGGKERDLYEFENLAYASGFSRCAIVCAVYPFSVIEIY 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B0ZB573.5e-10955.21Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1[more]
B6VJS43.3e-10754.70Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1... [more]
Q6VMW01.4e-9749.458-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=... [more]
A0A0N9HMN66.4e-9547.04Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P... [more]
Q84KK48.7e-9247.77Isoflavone 4'-O-methyltransferase OS=Lotus japonicus OX=34305 GN=HI4'OMT PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1GLH71.8e-209100.00trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1GLE59.1e-19391.88probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455048 PE=... [more]
A0A5A7UZZ22.1e-14470.41Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7UTA42.7e-14470.22Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1C1F34.9e-13869.01probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE... [more]
Match NameE-valueIdentityDescription
XP_022952340.13.8e-209100.00trans-resveratrol di-O-methyltransferase-like [Cucurbita moschata][more]
XP_023554531.14.3e-20598.04probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo][more]
KAG6572270.14.8e-20496.64Trans-resveratrol di-O-methyltransferase, partial [Cucurbita argyrosperma subsp.... [more]
XP_022952339.11.9e-19291.88probable O-methyltransferase 3 [Cucurbita moschata][more]
XP_023554527.14.2e-19291.88probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G35160.19.7e-5436.07O-methyltransferase family protein [more]
AT4G35150.11.3e-4531.39O-methyltransferase family protein [more]
AT1G51990.13.8e-4234.22O-methyltransferase family protein [more]
AT1G51990.26.5e-4233.92O-methyltransferase family protein [more]
AT5G53810.15.7e-3832.27O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 108..357
e-value: 5.2E-83
score: 279.9
NoneNo IPR availablePIRSRPIRSR016958-1PIRSR016958-1coord: 183..303
e-value: 7.2E-5
score: 20.2
NoneNo IPR availablePIRSRPIRSR005739-1PIRSR005739-1coord: 26..356
e-value: 3.6E-89
score: 296.5
NoneNo IPR availablePANTHERPTHR11746:SF250O-METHYLTRANSFERASEcoord: 5..357
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 197..296
e-value: 6.44851E-4
score: 37.0243
IPR016461O-methyltransferase COMT-typePIRSFPIRSF005739O-mtasecoord: 2..357
e-value: 1.3E-125
score: 417.1
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 5..357
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 17..357
score: 79.130089
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 130..339
e-value: 1.2E-60
score: 204.4
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 3..106
e-value: 7.6E-35
score: 120.6
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 27..76
e-value: 3.6E-12
score: 46.1
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 7..110
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 111..354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh19G009180.1CmoCh19G009180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity