CmoCh19G005070 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh19G005070
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMethylmalonate-semialdehyde dehydrogenase (CoA acylating)
LocationCmo_Chr19: 6005476 .. 6006510 (-)
RNA-Seq ExpressionCmoCh19G005070
SyntenyCmoCh19G005070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAACTTTGTTTGCATTGTTTGGTTTCTGAGAAAACGTCAGGACAGGACGTGGAAAGTATGAGCTATGCTATCCTTTCTACGGTCCCAAATTTGCTTCGTTTTTGTGTTTGTAATTGTTTCTTGTAGAGACTGGCCTTCACTGCGTTAATTTCTGATTTGAAATAGTCTTCTTAACTTCTTGAAGTTTAGGAATTACTCTGTAGGTTGAAAGAAAGGGGAAATTTCCTGAAATTTTTTTTAGCTTGATCACCAATGTAGTTTGTCTTTCAATTCATGACCATGATATCATATATAATGTCAGCGCATACTTGTTTCATCTAGCGTTGTTACGAAAGGCTGATATATACTTCATTTACGCAAGCCTTCTCAATCCTCAGCATTCATAGATGTCATAAACCTAGTGAGTTACCAGCTCATTTTTACGCACTCTTTTCTATTTCAGATTTGAGAAAGTTAAGTTCCTTTAGGTACGGGTACGAAAATGTGCTTTTGTTTATAGGCAAGGAAGTTGTTTCTCAAGTTCCATTGACCACAAATGAAGAATTTAAAGCTGCAGTATCTGCTGCAATGCAAGCTTTTCCTGCTTGGCGTAATACTCCAGTTACAACTCGTCAACGAATTATGCTCAAGCTTCAAGAACTCGTTAGGCGAGATATCGTAAGTTCGTTTTTCAGTAAAAGGAATATTCCAAATTCACTTCAAAAGTGGATTTCCCCCCCTCTCATTTCAGGACAAACTTTCTCTAAATTTTACAACAGAACAAGGAAAAACATTAAAGGATGCACATGGAGATGTATCCCATGGGATAGGTTATAATCCTTTAGTTCCTTCCTCTCCCTGTGTATGCTTATGATTATGTGATGGAAAAATTCGTTTTATCATATGGCCTGAGCTGAATGTTCGATAGAAAATTGAAATGATTTATCTTATGTAGAGGTTGTGGAACATGCATGTGGAATGGCATCGCTGCAAATGGGGTAGTATGTCTCTCATGTGTCACATGGAATTGTTGATACCTGCAGCATCATAG

mRNA sequence

ATGAGGAACTTTGTTTGCATTGTTTGGTTTCTGAGAAAACATTTGAGAAAGTTAAGTTCCTTTAGGTACGGGTACGAAAATGTGCTTTTGTTTATAGGCAAGGAAGTTGTTTCTCAAGTTCCATTGACCACAAATGAAGAATTTAAAGCTGCAGTATCTGCTGCAATGCAAGCTTTTCCTGCTTGGCGTAATACTCCAGTTACAACTCGTCAACGAATTATGCTCAAGCTTCAAGAACTCGTTAGGCGAGATATCAGGTTGTGGAACATGCATGTGGAATGGCATCGCTGCAAATGGGGTAGTATGTCTCTCATGTGTCACATGGAATTGTTGATACCTGCAGCATCATAG

Coding sequence (CDS)

ATGAGGAACTTTGTTTGCATTGTTTGGTTTCTGAGAAAACATTTGAGAAAGTTAAGTTCCTTTAGGTACGGGTACGAAAATGTGCTTTTGTTTATAGGCAAGGAAGTTGTTTCTCAAGTTCCATTGACCACAAATGAAGAATTTAAAGCTGCAGTATCTGCTGCAATGCAAGCTTTTCCTGCTTGGCGTAATACTCCAGTTACAACTCGTCAACGAATTATGCTCAAGCTTCAAGAACTCGTTAGGCGAGATATCAGGTTGTGGAACATGCATGTGGAATGGCATCGCTGCAAATGGGGTAGTATGTCTCTCATGTGTCACATGGAATTGTTGATACCTGCAGCATCATAG

Protein sequence

MRNFVCIVWFLRKHLRKLSSFRYGYENVLLFIGKEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDIRLWNMHVEWHRCKWGSMSLMCHMELLIPAAS
Homology
BLAST of CmoCh19G005070 vs. ExPASy Swiss-Prot
Match: Q0WM29 (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ALDH6B2 PE=1 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 6.7e-17
Identity = 42/59 (71.19%), Postives = 52/59 (88.14%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDIRLWNMHV 93
           +EVVS+VPLTTNEEFKAAVSAA QAFP WRNTP+TTRQR+MLK QEL+R+++    M++
Sbjct: 139 QEVVSKVPLTTNEEFKAAVSAAKQAFPLWRNTPITTRQRVMLKFQELIRKNMDKLAMNI 197

BLAST of CmoCh19G005070 vs. ExPASy Swiss-Prot
Match: Q54I10 (Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Dictyostelium discoideum OX=44689 GN=mmsdh PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 8.2e-07
Identity = 24/52 (46.15%), Postives = 39/52 (75.00%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDI 86
           +E+V++VP++T EE +AAV AA  AFPAWR+T V+ R RI+   + L+ +++
Sbjct: 55  QELVTKVPVSTKEEMEAAVKAASDAFPAWRDTSVSNRSRIISNYKNLINKNM 106

BLAST of CmoCh19G005070 vs. ExPASy Swiss-Prot
Match: Q02253 (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Rattus norvegicus OX=10116 GN=Aldh6a1 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.1e-06
Identity = 22/52 (42.31%), Postives = 40/52 (76.92%), Query Frame = 0

Query: 35  EVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDIR 87
           EVV +VP +T  E +AAV+A  +AFPAW +T + +RQ+++L+ Q+L++ +++
Sbjct: 66  EVVGRVPQSTKAEMEAAVAACKRAFPAWADTSILSRQQVLLRYQQLIKENLK 117

BLAST of CmoCh19G005070 vs. ExPASy Swiss-Prot
Match: P52713 (Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Caenorhabditis elegans OX=6239 GN=alh-8 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.4e-06
Identity = 25/52 (48.08%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 35  EVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDIR 87
           EV++ VP  T  E +AAV +A  AF  W+NT   TRQ+ M KLQ L++RD++
Sbjct: 56  EVIAMVPNATQAEMQAAVDSAKNAFNTWKNTSPLTRQQCMFKLQALIKRDMK 107

BLAST of CmoCh19G005070 vs. ExPASy Swiss-Prot
Match: Q02252 (Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Homo sapiens OX=9606 GN=ALDH6A1 PE=1 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 3.1e-06
Identity = 28/87 (32.18%), Postives = 46/87 (52.87%), Query Frame = 0

Query: 19  SSFRYGYENVLLFIG-------------------KEVVSQVPLTTNEEFKAAVSAAMQAF 78
           SSF      V LFIG                    EV+ +VP  T  E  AA+++  +AF
Sbjct: 31  SSFSSSVPTVKLFIGGKFVESKSDKWIDIHNPATNEVIGRVPQATKAEMDAAIASCKRAF 90

Query: 79  PAWRNTPVTTRQRIMLKLQELVRRDIR 87
           PAW +T V +RQ+++L+ Q+L++ +++
Sbjct: 91  PAWADTSVLSRQQVLLRYQQLIKENLK 117

BLAST of CmoCh19G005070 vs. ExPASy TrEMBL
Match: A0A6J1JI32 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Cucurbita maxima OX=3661 GN=LOC111485986 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.4e-17
Identity = 48/52 (92.31%), Postives = 51/52 (98.08%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDI 86
           +EVVSQVPLTTNEEFKAAVSAA QAFPAWRNTPVTTRQR+MLKLQEL+RRDI
Sbjct: 67  QEVVSQVPLTTNEEFKAAVSAAKQAFPAWRNTPVTTRQRVMLKLQELIRRDI 118

BLAST of CmoCh19G005070 vs. ExPASy TrEMBL
Match: A0A6J1C484 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Momordica charantia OX=3673 GN=LOC111008344 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.4e-17
Identity = 48/52 (92.31%), Postives = 51/52 (98.08%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDI 86
           +EVVSQVPLTTNEEFKAAVSAA QAFPAWRNTPVTTRQR+MLKLQEL+RRDI
Sbjct: 67  QEVVSQVPLTTNEEFKAAVSAAKQAFPAWRNTPVTTRQRVMLKLQELIRRDI 118

BLAST of CmoCh19G005070 vs. ExPASy TrEMBL
Match: A0A6J1EIR0 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Cucurbita moschata OX=3662 GN=LOC111434633 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.4e-17
Identity = 48/52 (92.31%), Postives = 51/52 (98.08%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDI 86
           +EVVSQVPLTTNEEFKAAVSAA QAFPAWRNTPVTTRQR+MLKLQEL+RRDI
Sbjct: 67  QEVVSQVPLTTNEEFKAAVSAAKQAFPAWRNTPVTTRQRVMLKLQELIRRDI 118

BLAST of CmoCh19G005070 vs. ExPASy TrEMBL
Match: A0A7J6XDT9 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Thalictrum thalictroides OX=46969 GN=FRX31_002439 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.2e-16
Identity = 49/58 (84.48%), Postives = 52/58 (89.66%), Query Frame = 0

Query: 27  NVLLFIGKEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRD 85
           NVL    +EVVSQVPLTTNEEFKAAVSAA QAFP+WRNTPVTTRQRIM KLQEL+RRD
Sbjct: 59  NVLNPATQEVVSQVPLTTNEEFKAAVSAAKQAFPSWRNTPVTTRQRIMFKLQELIRRD 116

BLAST of CmoCh19G005070 vs. ExPASy TrEMBL
Match: A0A067E000 (Methylmalonate-semialdehyde dehydrogenase (CoA acylating) (Fragment) OS=Citrus sinensis OX=2711 GN=CISIN_1g0094071mg PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.5e-16
Identity = 48/68 (70.59%), Postives = 58/68 (85.29%), Query Frame = 0

Query: 25  YENVLLFIGKEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRD 84
           Y +V+    +EVVSQ+PLTTNEEFKAAVSAA QAFP+WRNTP+TTRQR+MLKLQEL+RRD
Sbjct: 57  YIDVINPATQEVVSQLPLTTNEEFKAAVSAAKQAFPSWRNTPITTRQRVMLKLQELIRRD 116

Query: 85  IRLWNMHV 93
           I    M++
Sbjct: 117 IDKLAMNI 124

BLAST of CmoCh19G005070 vs. NCBI nr
Match: XP_022927866.1 (methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Cucurbita moschata])

HSP 1 Score: 98.2 bits (243), Expect = 4.9e-17
Identity = 48/52 (92.31%), Postives = 51/52 (98.08%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDI 86
           +EVVSQVPLTTNEEFKAAVSAA QAFPAWRNTPVTTRQR+MLKLQEL+RRDI
Sbjct: 67  QEVVSQVPLTTNEEFKAAVSAAKQAFPAWRNTPVTTRQRVMLKLQELIRRDI 118

BLAST of CmoCh19G005070 vs. NCBI nr
Match: XP_023530795.1 (methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 98.2 bits (243), Expect = 4.9e-17
Identity = 48/52 (92.31%), Postives = 51/52 (98.08%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDI 86
           +EVVSQVPLTTNEEFKAAVSAA QAFPAWRNTPVTTRQR+MLKLQEL+RRDI
Sbjct: 67  QEVVSQVPLTTNEEFKAAVSAAKQAFPAWRNTPVTTRQRVMLKLQELIRRDI 118

BLAST of CmoCh19G005070 vs. NCBI nr
Match: KAG6588740.1 (Dynamin-related protein 3A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 98.2 bits (243), Expect = 4.9e-17
Identity = 48/52 (92.31%), Postives = 51/52 (98.08%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDI 86
           +EVVSQVPLTTNEEFKAAVSAA QAFPAWRNTPVTTRQR+MLKLQEL+RRDI
Sbjct: 67  QEVVSQVPLTTNEEFKAAVSAAKQAFPAWRNTPVTTRQRVMLKLQELIRRDI 118

BLAST of CmoCh19G005070 vs. NCBI nr
Match: XP_022988736.1 (methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Cucurbita maxima])

HSP 1 Score: 98.2 bits (243), Expect = 4.9e-17
Identity = 48/52 (92.31%), Postives = 51/52 (98.08%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDI 86
           +EVVSQVPLTTNEEFKAAVSAA QAFPAWRNTPVTTRQR+MLKLQEL+RRDI
Sbjct: 67  QEVVSQVPLTTNEEFKAAVSAAKQAFPAWRNTPVTTRQRVMLKLQELIRRDI 118

BLAST of CmoCh19G005070 vs. NCBI nr
Match: XP_022136696.1 (methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Momordica charantia])

HSP 1 Score: 98.2 bits (243), Expect = 4.9e-17
Identity = 48/52 (92.31%), Postives = 51/52 (98.08%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDI 86
           +EVVSQVPLTTNEEFKAAVSAA QAFPAWRNTPVTTRQR+MLKLQEL+RRDI
Sbjct: 67  QEVVSQVPLTTNEEFKAAVSAAKQAFPAWRNTPVTTRQRVMLKLQELIRRDI 118

BLAST of CmoCh19G005070 vs. TAIR 10
Match: AT2G14170.1 (aldehyde dehydrogenase 6B2 )

HSP 1 Score: 88.2 bits (217), Expect = 4.7e-18
Identity = 42/59 (71.19%), Postives = 52/59 (88.14%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDIRLWNMHV 93
           +EVVS+VPLTTNEEFKAAVSAA QAFP WRNTP+TTRQR+MLK QEL+R+++    M++
Sbjct: 139 QEVVSKVPLTTNEEFKAAVSAAKQAFPLWRNTPITTRQRVMLKFQELIRKNMDKLAMNI 197

BLAST of CmoCh19G005070 vs. TAIR 10
Match: AT2G14170.2 (aldehyde dehydrogenase 6B2 )

HSP 1 Score: 88.2 bits (217), Expect = 4.7e-18
Identity = 42/59 (71.19%), Postives = 52/59 (88.14%), Query Frame = 0

Query: 34 KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDIRLWNMHV 93
          +EVVS+VPLTTNEEFKAAVSAA QAFP WRNTP+TTRQR+MLK QEL+R+++    M++
Sbjct: 30 QEVVSKVPLTTNEEFKAAVSAAKQAFPLWRNTPITTRQRVMLKFQELIRKNMDKLAMNI 88

BLAST of CmoCh19G005070 vs. TAIR 10
Match: AT2G14170.3 (aldehyde dehydrogenase 6B2 )

HSP 1 Score: 88.2 bits (217), Expect = 4.7e-18
Identity = 42/59 (71.19%), Postives = 52/59 (88.14%), Query Frame = 0

Query: 34  KEVVSQVPLTTNEEFKAAVSAAMQAFPAWRNTPVTTRQRIMLKLQELVRRDIRLWNMHV 93
           +EVVS+VPLTTNEEFKAAVSAA QAFP WRNTP+TTRQR+MLK QEL+R+++    M++
Sbjct: 139 QEVVSKVPLTTNEEFKAAVSAAKQAFPLWRNTPITTRQRVMLKFQELIRKNMDKLAMNI 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WM296.7e-1771.19Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Arabidop... [more]
Q54I108.2e-0746.15Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS... [more]
Q022531.1e-0642.31Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Rattus n... [more]
P527131.4e-0648.08Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS... [more]
Q022523.1e-0632.18Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial OS=Homo sap... [more]
Match NameE-valueIdentityDescription
A0A6J1JI322.4e-1792.31Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Cucurbita maxima OX... [more]
A0A6J1C4842.4e-1792.31Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Momordica charantia... [more]
A0A6J1EIR02.4e-1792.31Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Cucurbita moschata ... [more]
A0A7J6XDT91.2e-1684.48Methylmalonate-semialdehyde dehydrogenase (CoA acylating) OS=Thalictrum thalictr... [more]
A0A067E0001.5e-1670.59Methylmalonate-semialdehyde dehydrogenase (CoA acylating) (Fragment) OS=Citrus s... [more]
Match NameE-valueIdentityDescription
XP_022927866.14.9e-1792.31methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Cucurbita ... [more]
XP_023530795.14.9e-1792.31methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Cucurbita ... [more]
KAG6588740.14.9e-1792.31Dynamin-related protein 3A, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022988736.14.9e-1792.31methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Cucurbita ... [more]
XP_022136696.14.9e-1792.31methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial [Momordica ... [more]
Match NameE-valueIdentityDescription
AT2G14170.14.7e-1871.19aldehyde dehydrogenase 6B2 [more]
AT2G14170.24.7e-1871.19aldehyde dehydrogenase 6B2 [more]
AT2G14170.34.7e-1871.19aldehyde dehydrogenase 6B2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015590Aldehyde dehydrogenase domainPFAMPF00171Aldedhcoord: 34..84
e-value: 8.2E-10
score: 37.8
IPR016162Aldehyde dehydrogenase, N-terminalGENE3D3.40.605.10Aldehyde Dehydrogenase; Chain A, domain 1coord: 26..94
e-value: 4.1E-12
score: 47.8
NoneNo IPR availablePANTHERPTHR43866:SF1METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL-LIKEcoord: 33..92
IPR010061Methylmalonate-semialdehyde dehydrogenasePANTHERPTHR43866MALONATE-SEMIALDEHYDE DEHYDROGENASEcoord: 33..92
IPR016161Aldehyde/histidinol dehydrogenaseSUPERFAMILY53720ALDH-likecoord: 33..86

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh19G005070.1CmoCh19G005070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006210 thymine catabolic process
biological_process GO:0006574 valine catabolic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0018478 malonate-semialdehyde dehydrogenase (acetylating) activity
molecular_function GO:0004491 methylmalonate-semialdehyde dehydrogenase (acylating) activity
molecular_function GO:0016491 oxidoreductase activity