CmoCh19G004670 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh19G004670
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	beta-amyrin 11-oxidase-like
Location	Cmo_Chr19: 5611135 .. 5616379 (+)
RNA-Seq Expression	CmoCh19G004670
Synteny	CmoCh19G004670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAACGATTAATGTTTGTGTTATTATTGGATTTGTTTTAGGGGTTTATATGTTTGTGAGGAAACTCAATGAAATATGGTATTTGGTTAAGTTGGGAAGAAAGGCTTACAAATCTCTTCCTCCTGGTGATATGGGATGGCCTCTTCTTGGCTCTTCCTTCTCCTTTTACAATGCATTTACGGTCGGAGGTGATCCCGACTCTTTTATTTATCACCTTCTTTCCAGGTATATCTCTTCATTTCTCTTTATCGGGTCATGTGTATCGTTATTATATGCATGTCATGAAAGACTTCTCAAAACTAATTTGAATAGAGGTATATGAAAACTTAAACGTTTTAAAACTCCTTATATTTATGTTTGAAATTCTAGAAAAAATCTTAAAATGATAAATGATATGCTTTAAACATCATAATCAAAATTTTAGTATTCATCAAAACATTTAAAATATTATTGTCAAATACCGTAACTAAAATCTAAAAATAAAAATAAAAAGGTGAACCATGACCACCAAAAACGTCCTTGTCCTTGTCCTTGTCTAAAAAAAATTAAGTTTTGAAAATGGTGGTTGGATCAAAAAATTCAAACATATACAAATATGACCATCCTTACTTGTGGGACCTAAACCAACTCAACTTATTATGTTGATAAAAAGTATATGTGCATTACTTATATATACATGATTCATATACATGCACATGAACTTGCTAAGTAAGCATTACCTTCATTCTTTTGAGGCATGACACGGCCGAAATGATCAAAAGAAAAGAATTTATTTTTTTCTGGACAAATTACTATTTATTTCTTACAAACACCTCATTGATCTTTTTTAATAAGTTTGATGAAATCGAGAATTATTGTAATAGTTCAAGTCCACTACTAGTAAATATTTTCCACTTTTGCCTGTTACATTATCATAGGGATAAGTTTCCCTACTCTTATAAAGAATGGTTCCCTTCATTCCATCAACTTCACGATATTCAAAACGCGTCCACTAAGGAAAGATTTTCACACCCTTTTAAGGAATGGTTCCTTTTTTTGTTTCCCTCTCCAACCAATGTGAGATCTCACAATCTACCCCTTGGGGGCCAACGTCCTCATTGACACACCGTTCGGTATCATCCTCATGATTTTTAAAACCTCACAATCCACCCTCTTGGAAGCCAACGTCATCGCTGGCACACCGCCTGGTAAAATCTCACGATTTTTAAAACGCATCTGCTAGAAAAAAATTTCTACACACTTGGGTGTTTGGTTCCCCTCTCCAATCGAAACGAGATCTCATTTAGAAAAGATTATTGCATTCTTGCACAGGAATGGAAGAGTCGGGATGTATAAAAGTCACTTCTATGGAAGGCCAACAATCATCGTAACAGATCCAGAGATATGTCGACGCATATATTTAGATGATGAAATCTTCAAACCAAATTATCCAAAATCGGTGAAGATATTAGAAGTAAATGGTTTTTTCACAAGGGCTGATCACAAATCTGCATATAGAATCATGGCAGCTCCCATGAATGGCTATGAGGTATTGTCTCATTATGTGGACTTCATTGATCAAGTAATGGCAAAAGGCTTGGAGGAATGGAGCAGCATGAAAGAGCCCATTGAGTTGTTGGGTGAGATTGGAAGCCTCTTTTTTAAAGTAATTTCACGTATTTTCTTGGGGACTGACATTGGAGCATCTCGCATGGCTGAGCTTGAGACATTATATAAAGATTTGGGTCCTGCAATCTTGTCCATTCTTCCTTATGACTTGCCTGGACTCACTTTTCATGGCGCACTTAAGGTACCATTATTAGTGTTGTAAGCTGTGGTAAAGAATAAGTTCATGATCTTCTATGTATATATTGACATTTATTTGGTTCGTAATTCCCATTTATTTGGATCATCTCACAGGTTCATAACTCTTTTGGTTCACATCTCTCATTTATTTGGTTAATCTCTCATTCATTTGATTATCTTCCATTTTAATACATGAATCATCTTAATTCATGTATGATAATAATACTTTTATGTCTATATAAAGAGTTGTCTAGGACTTCTATGTATATATTGACATTTATTTGGTTCGTAATTCCCATTTATTTAGATCACCTCTCATTTATTTGGTTCATAACCCTTTTGGTTCACATCTCTGATTTATTTAGTTAATCTCTCATTCATTTGATTATCTTCCATTTTAATACATGAATCATTTTAATTCATGTATGATAATAATACTTTTCTGTCTATATAAAGAGTTGTCTAGGATGATTGAGATATGTATAAAGTTTGCTTGAATTTGAGTGTCATTTTGTATTTGTTGAATGAAATTTGAGAAGAACTTTATTGGGAGACTTTCTGTGTGAGATATAGACTTTGTAAATATTTTGATTATTAAGGTGGGTTGCTACCCATGGATGTAGGGGAATTTCTTCTCTCCAAACCACAAATTTTTGTATCTCTTCGTTTAATTTCTGTTTGTGGGCATGTGAGGGTGACCGATCTTACTTCTGCTTCCGTCTTCCGTCCAACAATTACTTCATATTGAATACGTTTTTTGGGGCATTATTTATTTACTTATGCTAATGGATGTGATGGGAAGATATTAAATGTTATTTTGTATACAACATATTGGCAGGCAAGAAAAAAGATAGAAAATATTCTACAATGTGTGATAGAGGAGAAAAGAAGAGCCCTAGAAAAGGAAGAAAGAGAAGTGGAGAGCCAAATGGACAAATTGATTATGGCAAGGGATGAGAATGGGATGAAATTATATGATAACAATGCCATAATAGACCTGCTTCTTGGACTGCTACATGCTGGCCATCATACCCCAGCTTATGCTGCTATGTGGGCTCTTGTCCACATCTCTGAGAAACCTCATGTCTTTCACAAGGCTAAGGTTAGCTCATTCTTTTAAAACCACTTTTAATTTCTTTTGTAATGGTTTGAGTTACGTTTTACAGGAAGAGCAAGAGTCGATTATAAAACAAAGACCATCTACTCAAAAGGGATTAACTTTTCAAGAAATCAAGCAGATGAAATATCTTATGAAGGTATGTCTTTTTTCTAAAAAAAAAAATTCGTAGAAAGTAAACCAATGAAATCCAAAGAAGCTATAAGAACCTGGATCAGTTTTAAAGGACATTTTTCACCAGTGAAATGCAAAGAAGCTATAAGAACTTGGATCAGTTTTAACTCACACAAGTCGTTCGAGACATGTAAAGTGCTCTAACTAGGTTGTAGGAAAATGTCGAGGCTGTGTATCGAATCTGGATTCTAAATCTAGGGCTTAGGAGCCTTACCTCCTACTAAAAAAATCTTCTACCAAGTAGAAAAATTAGGTTAATGAACACATTTTAACAAGAACGTAGAGTCAGTCATCACATTTCAAGTAACACCTCGTGTCCAAGATTCATCTTTTTTTTCATACTTGTGTGCTTTGACATGCTCCTGCAACATGGTTACATTACTTACTTCTTGGTTCTAAGAGTGAAAAGTATCCCAATAGATCAACACGAATTTTGTCGTCGTGTTTTATTCTCACTCCCATGCATTTTAGGAAAATTTTCAAGAGATAACCAAACATTTAATTACTCACAGTTTCTATGATTGAGTCACAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATTGAGTCACAAAAAAAAAAAAAAAAAAAGTAAACATATAATTACTCAAAGGTATTAACTTTCCATTTTTTTAAAAGTTTTTCATAGTCATTTTATCCTCAATATCACTCTCATTCAAATATAGTATCGGTTCATTTATGCATCCCTCCTTTATTCGGGTATCACATTTCATCGATATTAGAGATAAAATAGAGAATATAAATTTTGAGTTTGATTAATTTATCGTTTCAGTTTATTAATGAGCTATTGAGAAGAAACACCATCGCCGTTGCAAGTTTCCGCAAAGCAAAAAAAGATGTAAACATCAATGGTAAGTTATTCTATTAATGTTTGATATATAATTACATGACATTTATAATTTGTTTGTTGATGTTTTCGTATTTGTTTTAGACTACATCATACCAAAGGGATGGAACGTACTAATTTGGACAAGAGCTATTCATATGGACCCTCAATTATATTCAAATCCACAAGAGTTTGATCCTTCGAGATGGGAGGTATGATTTATGATCCCAATCAATTCATCAATTCAAATGGTTGGATGAAAGTCTCATATCAATTAATTTAGGAAATTATCAAAGAATACTATTTCTATTAGTACGAGGCTTTTTGGGAAGCTCAAAGCAAAGCTATGAGAGCTTATACTCAAAGTGGACAATATCACGTCATTATGGAGAGTCGTGTCCATCTAATATGGTATCAGACCCATGCCCTAGATTTAGCCATGTCAATAAAATCCTCAAATGTCAAACAAAGGACTCCAGAAGAAAAAGAGTCGAGCCTCAATTAAAGGGAGGTGTACTTTGTTCGAGAGGAGGTGTAATTTAGGAAATGATCATGAATTTATAAAAGAATATTATACTCAAAGTAGACAATATTATACTATTGTGGAGAGTCGTGTTCATTTAACAAAGATGTTTATCATTAAAATATTAACGTGGGTGGTGTTTATAAGCAGAATTATTCACCAAAGCCAGGAACATTCCTCCCTTTTGGAATCGGAACTAGGTTTTGTCCTGGAAGTGAGCTTACCAAGCTTGAGATTACAATTTTACTTCATCATTTTATCCTGAATTACAAGTAAATACCCATCCAAATCTATCTTTTTACTGTTTAATCAAAATAAAAATGCATGTGATGGAGTTGTTTTGTTTGTGTTGTTGTAGAATGGAACGAGTCAATCCAAAATGCTGCATCACTCACTTACCTGCCCCCAAGCTCGTAGACAACTGCCTTTGCACAATTACCAAGCTCCCATAG

mRNA sequence

ATGGAAACGATTAATGTTTGTGTTATTATTGGATTTGTTTTAGGGGTTTATATGTTTGTGAGGAAACTCAATGAAATATGGTATTTGGTTAAGTTGGGAAGAAAGGCTTACAAATCTCTTCCTCCTGGTGATATGGGATGGCCTCTTCTTGGCTCTTCCTTCTCCTTTTACAATGCATTTACGGTCGGAGGTGATCCCGACTCTTTTATTTATCACCTTCTTTCCAGGCCAACAATCATCGTAACAGATCCAGAGATATGTCGACGCATATATTTAGATGATGAAATCTTCAAACCAAATTATCCAAAATCGGTGAAGATATTAGAAGTAAATGGTTTTTTCACAAGGGCTGATCACAAATCTGCATATAGAATCATGGCAGCTCCCATGAATGGCTATGAGGTATTGTCTCATTATGTGGACTTCATTGATCAAGTAATGGCAAAAGGCTTGGAGGAATGGAGCAGCATGAAAGAGCCCATTGAGTTGTTGGGTGAGATTGGAAGCCTCTTTTTTAAAGTAATTTCACGTATTTTCTTGGGGACTGACATTGGAGCATCTCGCATGGCTGAGCTTGAGACATTATATAAAGATTTGGGTCCTGCAATCTTGTCCATTCTTCCTTATGACTTGCCTGGACTCACTTTTCATGGCGCACTTAAGGCAAGAAAAAAGATAGAAAATATTCTACAATGTGTGATAGAGGAGAAAAGAAGAGCCCTAGAAAAGGAAGAAAGAGAAGTGGAGAGCCAAATGGACAAATTGATTATGGCAAGGGATGAGAATGGGATGAAATTATATGATAACAATGCCATAATAGACCTGCTTCTTGGACTGCTACATGCTGGCCATCATACCCCAGCTTATGCTGCTATGTGGGCTCTTGTCCACATCTCTGAGAAACCTCATGTCTTTCACAAGGCTAAGGAAGAGCAAGAGTCGATTATAAAACAAAGACCATCTACTCAAAAGGGATTAACTTTTCAAGAAATCAAGCAGATGAAATATCTTATGAAGACTACATCATACCAAAGGGATGGAACGTTTTGTCCTGGAAGTGAGCTTACCAAGCTTGAGATTACAATTTTACTTCATCATTTTATCCTGAATTACAAAATGGAACGAGTCAATCCAAAATGCTGCATCACTCACTTACCTGCCCCCAAGCTCGTAGACAACTGCCTTTGCACAATTACCAAGCTCCCATAG

Coding sequence (CDS)

ATGGAAACGATTAATGTTTGTGTTATTATTGGATTTGTTTTAGGGGTTTATATGTTTGTGAGGAAACTCAATGAAATATGGTATTTGGTTAAGTTGGGAAGAAAGGCTTACAAATCTCTTCCTCCTGGTGATATGGGATGGCCTCTTCTTGGCTCTTCCTTCTCCTTTTACAATGCATTTACGGTCGGAGGTGATCCCGACTCTTTTATTTATCACCTTCTTTCCAGGCCAACAATCATCGTAACAGATCCAGAGATATGTCGACGCATATATTTAGATGATGAAATCTTCAAACCAAATTATCCAAAATCGGTGAAGATATTAGAAGTAAATGGTTTTTTCACAAGGGCTGATCACAAATCTGCATATAGAATCATGGCAGCTCCCATGAATGGCTATGAGGTATTGTCTCATTATGTGGACTTCATTGATCAAGTAATGGCAAAAGGCTTGGAGGAATGGAGCAGCATGAAAGAGCCCATTGAGTTGTTGGGTGAGATTGGAAGCCTCTTTTTTAAAGTAATTTCACGTATTTTCTTGGGGACTGACATTGGAGCATCTCGCATGGCTGAGCTTGAGACATTATATAAAGATTTGGGTCCTGCAATCTTGTCCATTCTTCCTTATGACTTGCCTGGACTCACTTTTCATGGCGCACTTAAGGCAAGAAAAAAGATAGAAAATATTCTACAATGTGTGATAGAGGAGAAAAGAAGAGCCCTAGAAAAGGAAGAAAGAGAAGTGGAGAGCCAAATGGACAAATTGATTATGGCAAGGGATGAGAATGGGATGAAATTATATGATAACAATGCCATAATAGACCTGCTTCTTGGACTGCTACATGCTGGCCATCATACCCCAGCTTATGCTGCTATGTGGGCTCTTGTCCACATCTCTGAGAAACCTCATGTCTTTCACAAGGCTAAGGAAGAGCAAGAGTCGATTATAAAACAAAGACCATCTACTCAAAAGGGATTAACTTTTCAAGAAATCAAGCAGATGAAATATCTTATGAAGACTACATCATACCAAAGGGATGGAACGTTTTGTCCTGGAAGTGAGCTTACCAAGCTTGAGATTACAATTTTACTTCATCATTTTATCCTGAATTACAAAATGGAACGAGTCAATCCAAAATGCTGCATCACTCACTTACCTGCCCCCAAGCTCGTAGACAACTGCCTTTGCACAATTACCAAGCTCCCATAG

Protein sequence

METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDSFIYHLLSRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKTTSYQRDGTFCPGSELTKLEITILLHHFILNYKMERVNPKCCITHLPAPKLVDNCLCTITKLP

Homology

[Close]

BLAST of CmoCh19G004670 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 5.4e-74
Identity = 180/493 (36.51%), Postives = 267/493 (54.16%), Query Frame = 0


Query: 3   TINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTV 62
           T+ VC I G       FVR LN  +Y VKL RK +  LPPGDMGWPL+G   SF   F+ 
Sbjct: 12  TLLVCYIFG-----SKFVRNLNGWYYDVKLRRKEH-PLPPGDMGWPLIGDLLSFIKDFS- 71

Query: 63  GGDPDSFI--------------YHLLSRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK-- 122
            G PDSFI               HL   P+IIV +P++CRR+  DD  FK  YPKS+K  
Sbjct: 72  SGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKEL 131

Query: 123 -----ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPI 182
                +++V    + A+H+   R++ +P+ G++ L+ Y++ +++++   LEE SSMK P+
Sbjct: 132 ARCRPMIDV----SNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPV 191

Query: 183 ELLGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALK 242
           ELL E+  + FK I  +F+G+      + ++ + + DL   + SI P ++PG TFH AL+
Sbjct: 192 ELLKEMKKVSFKAIVHVFMGSS-NQDIIKKIGSSFTDLYNGMFSI-PINVPGFTFHKALE 251

Query: 243 ARKKIENILQCVIEEKRRALE---KEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLG 302
           ARKK+  I+Q V++E+R  +E   +E  + +  +D L+  +DENG KL D + I DLL+G
Sbjct: 252 ARKKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDED-ISDLLIG 311

Query: 303 LLHAGHHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYL- 362
           LL AGH + A + MW++ ++++ PH+  KAKEEQE I + R S+QK L+ +EIKQM YL 
Sbjct: 312 LLFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLS 371

Query: 363 ---------------------------------------------MKTTSY--------- 402
                                                        M +  Y         
Sbjct: 372 QVIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPS 431

[Close]

BLAST of CmoCh19G004670 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 5.4e-66
Identity = 163/485 (33.61%), Postives = 250/485 (51.55%), Query Frame = 0


Query: 8   VIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPD 67
           V+ G VL V   VR+ ++   +  LG +    LPPG+MGWP++GS ++F  AF   G+PD
Sbjct: 24  VVAGVVL-VDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFK-SGNPD 83

Query: 68  SFIYHLLSR--------------PTIIVTDPEICRRIYLDDEIFKPNYPK-SVKILEVNG 127
           +FI   + R              PTI+   PE C+++ +DDE F   +PK +V ++    
Sbjct: 84  AFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKS 143

Query: 128 FFTRA--DHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKE-PIELLGEIGS 187
           F   +  DH+   ++ AAP+NG++ L+ Y+ FIDQ +   L  WSS +   +E L E+  
Sbjct: 144 FVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRR 203

Query: 188 LFFKVISRIFL-GTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIEN 247
           + FK+I +IF+ G D   + M  LE  Y DL    +  +  +LPG  ++ AL+AR+K+ +
Sbjct: 204 MTFKIIVQIFMSGAD--DATMEALERSYTDLNYG-MRAMAINLPGFAYYRALRARRKLVS 263

Query: 248 ILQCVIEEKRRALEK--EEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 307
           +LQ V++ +R A  K  +       MD+LI A DE G +L D+  I+D+L+  L+AGH +
Sbjct: 264 VLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDE-IVDVLIMYLNAGHES 323

Query: 308 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKTTSY--- 367
             +  MWA V + E P +F +AK EQE I++  P+TQ GLT ++ K+M +L +       
Sbjct: 324 SGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLR 383

Query: 368 ----------------------------------------------------------QR 402
                                                                      +
Sbjct: 384 CVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPK 443

[Close]

BLAST of CmoCh19G004670 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.1e-62
Identity = 154/464 (33.19%), Postives = 238/464 (51.29%), Query Frame = 0


Query: 27  WYL-VKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDSFI--------------Y 86
           WY    LG      LPPG+MGWPL+G  ++F  AF   G PD+FI               
Sbjct: 58  WYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFK-SGKPDAFIASFVRRFGRTGVYRS 117

Query: 87  HLLSRPTIIVTDPEICRRIYLDDEIFKPNYPK-SVKILEVNGFFTRA--DHKSAYRIMAA 146
            + S PT++VT  E C+++ +DD+ F   +PK +V ++    F      +H+   ++ AA
Sbjct: 118 FMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRIRKLTAA 177

Query: 147 PMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKVISRIFLGTDIGASR 206
           P+NG++ L+ Y+ FID+ +   L  W+     +E L E+  + FK+I +IFLG    A+ 
Sbjct: 178 PINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGGADQATT 237

Query: 207 MAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENILQCVIEEKRRALEK-EERE 266
            A LE  Y +L    +  +  +LPG  + GAL+AR+++  +LQ V++E+R A  K     
Sbjct: 238 RA-LERSYTELNYG-MRAMAINLPGFAYRGALRARRRLVAVLQGVLDERRAARAKGVSGG 297

Query: 267 VESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAYAAMWALVHISEKPHVFHK 326
               MD+LI A+DE G  L D++ IID+L+  L+AGH +  +  MWA V + E P +F +
Sbjct: 298 GVDMMDRLIEAQDERGRHL-DDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPDMFAR 357

Query: 327 AKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKT--------------------------- 386
           AK EQE+I++  PS+Q+GLT ++ ++M+YL +                            
Sbjct: 358 AKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQATRDVFVNGY 417

Query: 387 -------------------------TSYQ---------RDGTF---------CPGSELTK 402
                                    T +          R GTF         CPG++L K
Sbjct: 418 LIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGARLCPGNDLAK 477

[Close]

BLAST of CmoCh19G004670 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 241.1 bits (614), Expect = 2.1e-62
Identity = 168/482 (34.85%), Postives = 245/482 (50.83%), Query Frame = 0


Query: 8   VIIGFVLGVYMFVRKLNEIW-YLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDP 67
           +I+G  L V  +V K   +W Y+ KLG K +  LPPGD+GWP++G+ +SF  AF    DP
Sbjct: 14  IILG--LFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 68  DSFIY--------------HLLSRPTIIVTDPEICRRIYLDDEIFKPNYPKS-VKILEVN 127
           +SFI               H+   P ++VT PE CRR+  DD+ F   +PKS +K++   
Sbjct: 74  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 133

Query: 128 GF--FTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGS 187
            F   +  +HK   R+ +AP+NG E LS Y+ FI++ +   LE+WS M E IE L  +  
Sbjct: 134 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 193

Query: 188 LFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENI 247
           L FKVI  IFL ++     M  LE  Y +L   + + +  +LPG  +H ALKARKK+   
Sbjct: 194 LTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALKARKKLVAA 253

Query: 248 LQCVIEEKRRALEKE-EREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPA 307
            Q ++  +R   ++      +  +D LI  +DENG ++ D+  IIDLLL  L+AGH +  
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 313

Query: 308 YAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKT-------- 367
           +  MWA + + E P +  KAKEEQE I+K+R   QK LT +E ++M YL +         
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373

Query: 368 ----TSYQ-------------------------------------------------RDG 401
               T+++                                                 + G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433

[Close]

BLAST of CmoCh19G004670 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 5.3e-61
Identity = 156/476 (32.77%), Postives = 230/476 (48.32%), Query Frame = 0


Query: 20  VRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDSFI--------- 79
           VR  +  ++   LG      LPPGDMGWPL+G  ++F  AF   G PDSFI         
Sbjct: 25  VRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFK-SGRPDSFIDSFARRFGR 84

Query: 80  -----YHLLSRPTIIVTDPEICRRIYLDDEIFKPNYPK-SVKILEVNGFFTRA--DHKSA 139
                  + S PTI+ T PE C+++ +DD+ F   +PK +V ++    F      +H+  
Sbjct: 85  AGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRL 144

Query: 140 YRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM--KEPIELLGEIGSLFFKVISRIFL 199
            ++ AAP+NG++ L+ Y+ FID  +   L  WS        E L E+  + F++I +IF+
Sbjct: 145 RKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFM 204

Query: 200 GTDIGASR--MAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENILQCVIEEKR 259
           G   GA     AELE  Y +L    +  +  DLPG  +H A++AR+++   LQ V++E+R
Sbjct: 205 G---GADERTAAELERTYTELNYG-MRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERR 264

Query: 260 RALEKEEREVES---QMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAYAAMWAL 319
               K      +    MD+LI   DE G +L D+  IID+L+  L+AGH +  +  MWA 
Sbjct: 265 ARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDE-IIDVLVMYLNAGHESSGHITMWAT 324

Query: 320 VHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKTTSY------------ 379
           V + E P +  KAK EQE+I++  P  QKGLT ++ ++M YL +                
Sbjct: 325 VFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVVDETLRFVNISFVSF 384

Query: 380 -------------------------------------------------QRDGTF----- 402
                                                             R GTF     
Sbjct: 385 RQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAGTFLPFGL 444

[Close]

BLAST of CmoCh19G004670 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 1.8e-213
Identity = 397/486 (81.69%), Postives = 398/486 (81.89%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60

Query: 61  TVGGDPDSFIYHLLS--------------RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
           TVGGDPDSFIYHLLS              RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE
Sbjct: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240

Query: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300
           ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT
Sbjct: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300

Query: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------- 360
           PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK       
Sbjct: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINELLR 360

Query: 361 --------------------------------TTSYQRD--------------------- 403
                                           T +   D                     
Sbjct: 361 RNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWENYSPK 420

[Close]

BLAST of CmoCh19G004670 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.5e-204
Identity = 379/487 (77.82%), Postives = 389/487 (79.88%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           ME INVC IIG VLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF
Sbjct: 1   MEMINVCTIIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60

Query: 61  TVGGDPDSFIYHLLS--------------RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
           TVGGDPDSFIYHLLS              RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE
Sbjct: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYD+PGL +H ALKARKKI
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKI 240

Query: 241 ENILQCVIEEKRRALE-KEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHH 300
           EN+L+CVIEEKRRALE KE REV SQMDKLIMARDENGMKLYDNN IIDLLLGLLHAGHH
Sbjct: 241 ENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAGHH 300

Query: 301 TPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------ 360
           TPAYAAMWALVHISEKPH+FHKAKEEQESI+KQRPSTQKGLTFQEIKQMKYLMK      
Sbjct: 301 TPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELL 360

Query: 361 ------TTSYQRDGT--------------------------------------------- 403
                   S+++  T                                             
Sbjct: 361 RRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWENHSP 420

[Close]

BLAST of CmoCh19G004670 vs. ExPASy TrEMBL
Match: A0A6J1GKS7 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454913 PE=3 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 3.7e-203
Identity = 370/459 (80.61%), Postives = 387/459 (84.31%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFY AF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60

Query: 61  TVGGDPDSFIYHLLSR--------------PTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
           +VGGD DSFIYHLL+R              PTIIVTDPEICRRIYLDDEIFKPNYP SVK
Sbjct: 61  SVGGDSDSFIYHLLTRNGRVGMYKSHLYGKPTIIVTDPEICRRIYLDDEIFKPNYPNSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNG F+R DHK AYRIMAAPMNGYEVLSHYVDFIDQ+MA+GLE+WS+ KEPIELLGE
Sbjct: 121 ILEVNGLFSRMDHKCAYRIMAAPMNGYEVLSHYVDFIDQIMAEGLEKWSTKKEPIELLGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IG LFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPG TFH ALKARKKI
Sbjct: 181 IGRLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGFTFHRALKARKKI 240

Query: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300
           ENILQCVIEEKR+ALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT
Sbjct: 241 ENILQCVIEEKRKALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300

Query: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------- 360
           P+YAAMWALVHIS+KPH+FHKAKEEQESI++QR STQKGL FQEIKQMKYL+K       
Sbjct: 301 PSYAAMWALVHISQKPHIFHKAKEEQESILQQRLSTQKGLNFQEIKQMKYLIKLINEVLR 360

Query: 361 -----TTSYQ----------------------RDGT---------FCPGSELTKLEITIL 403
                  S++                      + GT         FCPGSELTKLEITIL
Sbjct: 361 RNTIAVASFRKAKKDVNINDYTIPKGWNNYSPKPGTFIPFGIGTRFCPGSELTKLEITIL 420

[Close]

BLAST of CmoCh19G004670 vs. ExPASy TrEMBL
Match: A0A6J1GJP8 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.9e-202
Identity = 375/486 (77.16%), Postives = 386/486 (79.42%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           METINVC+IIGF+LGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFY AF
Sbjct: 1   METINVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60

Query: 61  TVGGDPDSFIYHLLSR--------------PTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
            VGGDPDSFIYHLLSR              PTIIVTDPEICRRIYLDDEIFKPNYPKSVK
Sbjct: 61  IVGGDPDSFIYHLLSRNGRVGMYKSHLYGSPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNG F+R DHK AYRIMAAP+NGY VLSHYVDFIDQ+MAKGLEEWS+ KEPIEL GE
Sbjct: 121 ILEVNGLFSRMDHKCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEEWSTKKEPIELFGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IGSLFF+VISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFH ALKARKKI
Sbjct: 181 IGSLFFRVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHRALKARKKI 240

Query: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300
           ENILQCVIEEKRRALEK+EREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLH GHHT
Sbjct: 241 ENILQCVIEEKRRALEKQEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHGGHHT 300

Query: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------- 360
           PAYAAMWALVHISE PHVFHKAKEEQESI+KQR STQKGLTFQEIKQMKYLMK       
Sbjct: 301 PAYAAMWALVHISETPHVFHKAKEEQESILKQRSSTQKGLTFQEIKQMKYLMKFINELLR 360

Query: 361 --------------------------------TTSYQRD--------------------- 403
                                           T +   D                     
Sbjct: 361 RNTIAVASFREAKTEININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDPSRWENHSPK 420

[Close]

BLAST of CmoCh19G004670 vs. ExPASy TrEMBL
Match: A0A6J1I8I5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 7.8e-201
Identity = 375/495 (75.76%), Postives = 386/495 (77.98%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFY AF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60

Query: 61  TVGGDPDSFIYHLLSR--------------PTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
           TVGGDPDSFIYHLLSR              PTIIVTDPEICRRIYLDDEIF+PNYPKSVK
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHLYGKPTIIVTDPEICRRIYLDDEIFQPNYPKSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNG F+R DH  AYRIMAAP+NGY VLSHYVDFIDQ+MAKGLE+WS+ KEPIELLGE
Sbjct: 121 ILEVNGLFSRMDHNCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEKWSTKKEPIELLGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYD+PGLTFH ALKARKKI
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLTFHRALKARKKI 240

Query: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300
           ENILQCVIEEKRRALEK+EREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLH GHHT
Sbjct: 241 ENILQCVIEEKRRALEKQEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHGGHHT 300

Query: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------- 360
           PAYAAMWALV I E PHVFHKAKEEQESI+KQRPSTQKGLTFQEIKQMKYLMK       
Sbjct: 301 PAYAAMWALVQIFETPHVFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELLR 360

Query: 361 -----------------------------------------TTSYQRD------------ 403
                                                    T +   D            
Sbjct: 361 RNTIAVASFREAKTDININGELFYYIVLDYTIPKGWTVLIWTRAIHMDPQLYSNPKEFDP 420

[Close]

BLAST of CmoCh19G004670 vs. NCBI nr
Match: XP_022952843.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 751.5 bits (1939), Expect = 3.7e-213
Identity = 397/486 (81.69%), Postives = 398/486 (81.89%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60

Query: 61  TVGGDPDSFIYHLLS--------------RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
           TVGGDPDSFIYHLLS              RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE
Sbjct: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240

Query: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300
           ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT
Sbjct: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300

Query: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------- 360
           PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK       
Sbjct: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINELLR 360

Query: 361 --------------------------------TTSYQRD--------------------- 403
                                           T +   D                     
Sbjct: 361 RNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWENYSPK 420

[Close]

BLAST of CmoCh19G004670 vs. NCBI nr
Match: XP_023511634.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 737.6 bits (1903), Expect = 5.5e-209
Identity = 385/459 (83.88%), Postives = 391/459 (85.19%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFY AF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60

Query: 61  TVGGDPDSFIYHLLS--------------RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
           TVGGDPDSFIYHLL+              RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK
Sbjct: 61  TVGGDPDSFIYHLLTRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNG F+R DHKSAYRIM APMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE
Sbjct: 121 ILEVNGLFSRIDHKSAYRIMVAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFH ALKARKKI
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHRALKARKKI 240

Query: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300
           ENIL+CVIEEKRRALEKEEREVESQMDKLIMARDE  MKLYDNNAIIDLLLGLLHAGHHT
Sbjct: 241 ENILECVIEEKRRALEKEEREVESQMDKLIMARDEKWMKLYDNNAIIDLLLGLLHAGHHT 300

Query: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------- 360
           PAYAAMWALVHISEK HVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK       
Sbjct: 301 PAYAAMWALVHISEKLHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINELLR 360

Query: 361 -----TTSYQ----------------------RDGT---------FCPGSELTKLEITIL 403
                  S++                      + GT         FCPGSELTKLEITIL
Sbjct: 361 RNTIAVASFRKAKKDVNINDYTIPKGWNNYSPKPGTFLPFGIGTRFCPGSELTKLEITIL 420

[Close]

BLAST of CmoCh19G004670 vs. NCBI nr
Match: XP_022952147.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 721.8 bits (1862), Expect = 3.1e-204
Identity = 379/487 (77.82%), Postives = 389/487 (79.88%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           ME INVC IIG VLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF
Sbjct: 1   MEMINVCTIIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60

Query: 61  TVGGDPDSFIYHLLS--------------RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
           TVGGDPDSFIYHLLS              RPTIIVTDPEICRRIYLDDEIFKPNYPKSVK
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE
Sbjct: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYD+PGL +H ALKARKKI
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKI 240

Query: 241 ENILQCVIEEKRRALE-KEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHH 300
           EN+L+CVIEEKRRALE KE REV SQMDKLIMARDENGMKLYDNN IIDLLLGLLHAGHH
Sbjct: 241 ENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAGHH 300

Query: 301 TPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------ 360
           TPAYAAMWALVHISEKPH+FHKAKEEQESI+KQRPSTQKGLTFQEIKQMKYLMK      
Sbjct: 301 TPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELL 360

Query: 361 ------TTSYQRDGT--------------------------------------------- 403
                   S+++  T                                             
Sbjct: 361 RRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWENHSP 420

[Close]

BLAST of CmoCh19G004670 vs. NCBI nr
Match: XP_022952149.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 717.2 bits (1850), Expect = 7.7e-203
Identity = 370/459 (80.61%), Postives = 387/459 (84.31%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFY AF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60

Query: 61  TVGGDPDSFIYHLLSR--------------PTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
           +VGGD DSFIYHLL+R              PTIIVTDPEICRRIYLDDEIFKPNYP SVK
Sbjct: 61  SVGGDSDSFIYHLLTRNGRVGMYKSHLYGKPTIIVTDPEICRRIYLDDEIFKPNYPNSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNG F+R DHK AYRIMAAPMNGYEVLSHYVDFIDQ+MA+GLE+WS+ KEPIELLGE
Sbjct: 121 ILEVNGLFSRMDHKCAYRIMAAPMNGYEVLSHYVDFIDQIMAEGLEKWSTKKEPIELLGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IG LFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPG TFH ALKARKKI
Sbjct: 181 IGRLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGFTFHRALKARKKI 240

Query: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300
           ENILQCVIEEKR+ALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT
Sbjct: 241 ENILQCVIEEKRKALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300

Query: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------- 360
           P+YAAMWALVHIS+KPH+FHKAKEEQESI++QR STQKGL FQEIKQMKYL+K       
Sbjct: 301 PSYAAMWALVHISQKPHIFHKAKEEQESILQQRLSTQKGLNFQEIKQMKYLIKLINEVLR 360

Query: 361 -----TTSYQ----------------------RDGT---------FCPGSELTKLEITIL 403
                  S++                      + GT         FCPGSELTKLEITIL
Sbjct: 361 RNTIAVASFRKAKKDVNINDYTIPKGWNNYSPKPGTFIPFGIGTRFCPGSELTKLEITIL 420

[Close]

BLAST of CmoCh19G004670 vs. NCBI nr
Match: XP_022952148.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 714.9 bits (1844), Expect = 3.8e-202
Identity = 375/486 (77.16%), Postives = 386/486 (79.42%), Query Frame = 0


Query: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
           METINVC+IIGF+LGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFY AF
Sbjct: 1   METINVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60

Query: 61  TVGGDPDSFIYHLLSR--------------PTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
            VGGDPDSFIYHLLSR              PTIIVTDPEICRRIYLDDEIFKPNYPKSVK
Sbjct: 61  IVGGDPDSFIYHLLSRNGRVGMYKSHLYGSPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180
           ILEVNG F+R DHK AYRIMAAP+NGY VLSHYVDFIDQ+MAKGLEEWS+ KEPIEL GE
Sbjct: 121 ILEVNGLFSRMDHKCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEEWSTKKEPIELFGE 180

Query: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240
           IGSLFF+VISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFH ALKARKKI
Sbjct: 181 IGSLFFRVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHRALKARKKI 240

Query: 241 ENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHT 300
           ENILQCVIEEKRRALEK+EREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLH GHHT
Sbjct: 241 ENILQCVIEEKRRALEKQEREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHGGHHT 300

Query: 301 PAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMK------- 360
           PAYAAMWALVHISE PHVFHKAKEEQESI+KQR STQKGLTFQEIKQMKYLMK       
Sbjct: 301 PAYAAMWALVHISETPHVFHKAKEEQESILKQRSSTQKGLTFQEIKQMKYLMKFINELLR 360

Query: 361 --------------------------------TTSYQRD--------------------- 403
                                           T +   D                     
Sbjct: 361 RNTIAVASFREAKTEININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDPSRWENHSPK 420

[Close]

BLAST of CmoCh19G004670 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 241.1 bits (614), Expect = 1.5e-63
Identity = 168/482 (34.85%), Postives = 245/482 (50.83%), Query Frame = 0


Query: 8   VIIGFVLGVYMFVRKLNEIW-YLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDP 67
           +I+G  L V  +V K   +W Y+ KLG K +  LPPGD+GWP++G+ +SF  AF    DP
Sbjct: 14  IILG--LFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 68  DSFIY--------------HLLSRPTIIVTDPEICRRIYLDDEIFKPNYPKS-VKILEVN 127
           +SFI               H+   P ++VT PE CRR+  DD+ F   +PKS +K++   
Sbjct: 74  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 133

Query: 128 GF--FTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGS 187
            F   +  +HK   R+ +AP+NG E LS Y+ FI++ +   LE+WS M E IE L  +  
Sbjct: 134 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 193

Query: 188 LFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENI 247
           L FKVI  IFL ++     M  LE  Y +L   + + +  +LPG  +H ALKARKK+   
Sbjct: 194 LTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALKARKKLVAA 253

Query: 248 LQCVIEEKRRALEKE-EREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPA 307
            Q ++  +R   ++      +  +D LI  +DENG ++ D+  IIDLLL  L+AGH +  
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 313

Query: 308 YAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKT-------- 367
           +  MWA + + E P +  KAKEEQE I+K+R   QK LT +E ++M YL +         
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373

Query: 368 ----TSYQ-------------------------------------------------RDG 401
               T+++                                                 + G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433

[Close]

BLAST of CmoCh19G004670 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 241.1 bits (614), Expect = 1.5e-63
Identity = 168/482 (34.85%), Postives = 245/482 (50.83%), Query Frame = 0


Query: 8   VIIGFVLGVYMFVRKLNEIW-YLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDP 67
           +I+G  L V  +V K   +W Y+ KLG K +  LPPGD+GWP++G+ +SF  AF    DP
Sbjct: 14  IILG--LFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 68  DSFIY--------------HLLSRPTIIVTDPEICRRIYLDDEIFKPNYPKS-VKILEVN 127
           +SFI               H+   P ++VT PE CRR+  DD+ F   +PKS +K++   
Sbjct: 74  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 133

Query: 128 GF--FTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGS 187
            F   +  +HK   R+ +AP+NG E LS Y+ FI++ +   LE+WS M E IE L  +  
Sbjct: 134 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 193

Query: 188 LFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENI 247
           L FKVI  IFL ++     M  LE  Y +L   + + +  +LPG  +H ALKARKK+   
Sbjct: 194 LTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRA-MGINLPGFAYHRALKARKKLVAA 253

Query: 248 LQCVIEEKRRALEKE-EREVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPA 307
            Q ++  +R   ++      +  +D LI  +DENG ++ D+  IIDLLL  L+AGH +  
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 313

Query: 308 YAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKT-------- 367
           +  MWA + + E P +  KAKEEQE I+K+R   QK LT +E ++M YL +         
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373

Query: 368 ----TSYQ-------------------------------------------------RDG 401
               T+++                                                 + G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433

[Close]

BLAST of CmoCh19G004670 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 234.6 bits (597), Expect = 1.4e-61
Identity = 158/474 (33.33%), Postives = 237/474 (50.00%), Query Frame = 0


Query: 15  GVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDSF----- 74
           G+   VRK+N   Y   LG   +  LPPGD+GWP +G+  SF  AF    DPDSF     
Sbjct: 21  GLNWLVRKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLI 80

Query: 75  -------IY--HLLSRPTIIVTDPEICRRIYLDDEIFKPNYPKS-VKILEVNGF--FTRA 134
                  IY  H+   P+IIVT  + CRR+  DD+ FKP +P S ++++    F   +  
Sbjct: 81  KRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFE 140

Query: 135 DHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKVISR 194
           +HK   R+ AAP+NG+E LS Y+ +I++ +   L++W+ M E  E L  +  L F++I  
Sbjct: 141 EHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMY 200

Query: 195 IFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKIENILQCVIEEK 254
           IFL ++   + M  LE  Y  L   + + +  ++PG  +H ALKARK +    Q ++ E+
Sbjct: 201 IFLSSE-SENVMDALEREYTALNYGVRA-MAVNIPGFAYHRALKARKTLVAAFQSIVTER 260

Query: 255 RRALEKEEREVESQM-DKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAYAAMWALV 314
           R   ++     +  M D L+  +DE+G K  D+  IID+LL  L+AGH +  +  MWA V
Sbjct: 261 RNQRKQNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMWATV 320

Query: 315 HISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYL------------------- 374
            + E P V  +AK EQE I+K RP  QKGL+ +E ++M++L                   
Sbjct: 321 FLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFR 380

Query: 375 ----------------MKTTSYQRD---------------------------GTF----- 400
                            K  ++ RD                           G F     
Sbjct: 381 EAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGA 440

[Close]

BLAST of CmoCh19G004670 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 103.6 bits (257), Expect = 3.8e-22
Identity = 110/468 (23.50%), Postives = 186/468 (39.74%), Query Frame = 0


Query: 25  EIWYLV----------------KLGRKAYKSLPPGDMGWPLLGSSFSFY----NAFTVGG 84
           EIW+LV                K  + +   LPPG MGWP LG +   Y    N F    
Sbjct: 3   EIWFLVVPILILCLLLVRVIVSKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSK 62

Query: 85  DP---DSFIYHLLSRPTIIVTDPEICRRIYLDD-EIFKPNYPKS-VKILEVNG-FFTRAD 144
                + F   +L  P +++  PE  R + +    +FKP YP+S  K++  +  FF + D
Sbjct: 63  QKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGD 122

Query: 145 HKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKV-ISR 204
           + S  R +       E +   +  I+ +    L+ W++M   +    E+    F V I  
Sbjct: 123 YHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKFAFDVGILA 182

Query: 205 IFLGTDIGASRMAELETLYKDLGPAILSIL-------PYDLPGLTFHGALKARKKIENIL 264
           IF            LE+ YK++     +I+       P  LPG ++H AL ARK+++ I+
Sbjct: 183 IF----------GHLESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIV 242

Query: 265 QCVIEEKRRALEKEEREVESQ-MDKLIMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAY 324
             +I E+R     E+R +++  +  L+  ++E G ++     I D ++G+L A   T A 
Sbjct: 243 SEIICERR-----EKRALQTDFLGHLLNFKNEKG-RVLTQEQIADNIIGVLFAAQDTTAS 302

Query: 325 AAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQM--------------- 384
              W L ++ +   +    K EQ++I ++    +K LT+++ + M               
Sbjct: 303 CLTWILKYLHDDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMAS 362

Query: 385 -------------------------------------KYLMKTTSYQ--------RDGTF 389
                                                KY      +         +  TF
Sbjct: 363 IISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTF 422

[Close]

BLAST of CmoCh19G004670 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 98.6 bits (244), Expect = 1.2e-20
Identity = 103/430 (23.95%), Postives = 171/430 (39.77%), Query Frame = 0


Query: 40  LPPGDMGWPLLGSSFSFYNAFTVGGDPDSFI------------YHLLSRPTIIVTDPEIC 99
           LPPG MG+P +G +F  Y+      DP+ F              H+L  P ++++ PE  
Sbjct: 36  LPPGTMGYPYVGETFQLYSQ-----DPNVFFAAKQRRYGSVFKTHVLGCPCVMISSPEAA 95

Query: 100 RRIYL-DDEIFKPNYPKSVKIL--EVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFID 159
           + + +    +FKP +P S + +  +   FF + D+ S  R +       + + + V  I+
Sbjct: 96  KFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIE 155

Query: 160 QVMAKGLEEWSSMKEPIELLGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAIL 219
            +  + L  W   +  +    E+ +  F V     LG D    R  +L+  Y  L     
Sbjct: 156 SIAQESLNSWDGTQ--LNTYQEMKTYTFNVALISILGKDEVYYR-EDLKRCYYILEKGYN 215

Query: 220 SILPYDLPGLTFHGALKARKKIENILQCVIEEKRRALEKEEREVESQMDKLIMARDENGM 279
           S +P +LPG  FH A+KARK++  IL  ++ ++R+        + S M+      DE   
Sbjct: 216 S-MPINLPGTLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGSFMEDKAGLTDEQ-- 275

Query: 280 KLYDNNAIIDLLLGLLHAGHHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQ------ 339
                  I D ++G++ A   T A    W L ++++ P V     EEQ +I K       
Sbjct: 276 -------IADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGES 335

Query: 340 --------------------RPSTQKGLTFQE-IKQMKY-------------LMKTTSYQ 387
                               R +T    TF+E ++ ++Y             L +   + 
Sbjct: 336 LTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHN 395

The following BLAST results are available for this feature:

BLAST of CmoCh19G004670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	5.4e-74	36.51	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q5VRM7	5.4e-66	33.61	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q43246	2.1e-62	33.19	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]
Q9C5Y2	2.1e-62	34.85	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q9AXH9	5.3e-61	32.77	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]

BLAST of CmoCh19G004670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GLR1	1.8e-213	81.69	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S...	[more]
A0A6J1GJM1	1.5e-204	77.82	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S...	[more]
A0A6J1GKS7	3.7e-203	80.61	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454913 PE=3 S...	[more]
A0A6J1GJP8	1.9e-202	77.16	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 S...	[more]
A0A6J1I8I5	7.8e-201	75.76	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=...	[more]

BLAST of CmoCh19G004670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022952843.1	3.7e-213	81.69	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]
XP_023511634.1	5.5e-209	83.88	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022952147.1	3.1e-204	77.82	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]
XP_022952149.1	7.7e-203	80.61	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]
XP_022952148.1	3.8e-202	77.16	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]

BLAST of CmoCh19G004670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	1.5e-63	34.85	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	1.5e-63	34.85	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	1.4e-61	33.33	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT3G19270.1	3.8e-22	23.50	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]
AT5G45340.1	1.2e-20	23.95	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 227..261
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 12..339 coord: 348..390
None	No IPR available	PANTHER	PTHR24286:SF289	ENT-KAURENOIC ACID OXIDASE 2	coord: 12..339 coord: 348..390
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 70..339 e-value: 2.2E-22 score: 79.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 32..341 e-value: 4.1E-43 score: 149.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 39..380

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh19G004670.1	CmoCh19G004670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0005783	endoplasmic reticulum
molecular_function	GO:0051777	ent-kaurenoate oxidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen