CmoCh19G003400 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh19G003400
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Cyclopropane-fatty-acyl-phospholipid synthase
Location	Cmo_Chr19: 2857355 .. 2859405 (+)
RNA-Seq Expression	CmoCh19G003400
Synteny	CmoCh19G003400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGCACGTAATGTTTTGTCTCTAATAGAAAAAGGAACACGCCTTTTTATTACAAGATTTCTTGGTCAATACATATCCACCGGATCGTTAACGTGAGCCAACACGATACAATATTTATGTGTTTGTTTTAATGAGAATTTATATTGCTTATAATAAATTTAATGACAGTAATATGAAGATGGGGGAGAAATATTTACCTTTGAGGGAACTGTAAGCAACAGCTTCTAACGTAAGGTTGTTTTAAAAGTTCACATCCCTCGTTTTATTGGAAGGCAATTTAATTATTTATTTAATTATTATTATTTTTTTAAAAAGTTGTTGAAAAAAAAAAGATAAAATATAATTTATTTTTACGATTACGAGAGAAGCTAGACTTGGGGCTTGCAGGTGCATATATCAATGGAGAATTTTCATTAATGGATAAAGAAGAGGGTCTTCTTAACCTTATTTTGGTGAGCTTAATTACTTCATTTCTTCCTAATCCTAATTAAGGTTCTTAATCATCGCCCTCGTTCATGCGCACCTTCTCTTTTGCACCTTCTCATTGCTAATAAGGATACAAATTCTTCTAATACAACACCAAACAAGACAATAAGGTAAATATGAATTATAAGACATTTTAATTACGAGTTTCATTTTTGAAGGAACTTAATTGTAACACTCCACCACTAACAAATATTATCAATTTTAGCGTGTTACGTATCGTCTTGTTACATATCGTCGTTAGACTCACAGTTTTAAAATTTATTTGTTACGGAGAGGTTTTCACTCCTTTATTAAAAAAAAAATGTATGTGTGTATCCTATCCACTCCCTTAGGGTCCAGCATCCTCGCTAGCACACCGTTCGGTGTCTTGCTCTGATACTATTTGTAATGGCCCATGACCACCACTAACAAATATTGTCTGTTTTAGCCCGGTTATGTATCGCCGTTCGCTTCATGGTTTTGAAACGTGTCTGCTTAGGAGAAATTTTCACACCCATATAAGAAATGTTTCGTTTTCCTATCCAATGGATGTGGATCTCACATTAAAAGTTTTTAATAGGTTCTTGAATTTACATTTTTGTGTTTCTAATATATTCATGAACTTTAACAAGTGTTTAATAAGTTTTGGAAATGACCCACTATCCAAATCTGTCAAAACAAATAAAGAGAGCATTAGCCTTCAATTCTCTCTGTTGTTATTATGAAAAAACATACCTCTCATGAGTATATATAGTAGAGAAAGTACTAACTAGCTTCGTAAACTTTCTCCTCAAGTTAAGAATTACTGAACGACGATCTTCATCGTTAAAGCATAAAACTTGTCTATCTACTACCAATTCTCCTATAGTTACTCCATAGGTTAGTAACTTATTCTATTATGTCTACTAACAATACCGTTAAAAGTGAGTGACTTACTTAAAAGCTCGTAATAAGTCTCTAAACATTCCATCTTTTATTTTCTTCAAACTTTTGATTTTATATTAATTCATCTATAAACTAGAAAATTTGTTAAACTTCATATGAATGGAAAGTTACAATAAATAAGTCATATACTTATTCAATATTTTTAAAATTGAATTTGTTAAACACGAACATAAAAATTAATTTAGGACAACAATAATAACATTGAACTGATAATTTTTTAATGTTGGTAATGGTGATCTACTTTTTGTAGGGGATGGAGGTCTCCTCCACTGTTTACCGCAACTATNTAAATTGGTAATTTATAATTGGCAGCGAGATGATAGAAGCAGTTGGCACGAATTCATAGAAGATTTCTTTGGTTGTTGTGAATCAGTGTTAGAGGAAAATGGTCTTCTTGTTCGGGGTTCAATTAATTTTTTTTTTTTAACTTTTTAGTGATAATTAATTTTTTTAATTACTCTTTATTTATTATTATTATTATTATTATTATTATTATTTTGCATTAGTTCATATTAGTGCCAGACGAGGAGCATCGGCTAAGTTCTGGTTTTATAAAAGAATATATATTTCCAGGAGAGTGCGTACCGTTATTAAGTAGAATAACAACGGCCATGGCTAGTGCATCAAGGCTTTGGTAA

mRNA sequence

ATGCAGGCACGTAATGTTTTGTCTCTAATAGAAAAAGGAACACGCCTTTTTATTACAAGATTTCTTGTTGTTGAAAAAAAAAAGATAAAATATAATTTATTTTTACGATTACGAGAGAAGCTAGACTTGGGGCTTGCAGGTGCATATATCAATGGAGAATTTTCATTAATGGATAAAGAAGAGGGTCTTCTTAACCTTATTTTGCGAGATGATAGAAGCAGTTGGCACGAATTCATAGAAGATTTCTTTGGTTGTTGTGAATCAGTGTTAGAGGAAAATGGTCTTCTTTTCATATTAGTGCCAGACGAGGAGCATCGGCTAAGTTCTGGTTTTATAAAAGAATATATATTTCCAGGAGAGTGCGTACCGTTATTAAGTAGAATAACAACGGCCATGGCTAGTGCATCAAGGCTTTGGTAA

Coding sequence (CDS)

ATGCAGGCACGTAATGTTTTGTCTCTAATAGAAAAAGGAACACGCCTTTTTATTACAAGATTTCTTGTTGTTGAAAAAAAAAAGATAAAATATAATTTATTTTTACGATTACGAGAGAAGCTAGACTTGGGGCTTGCAGGTGCATATATCAATGGAGAATTTTCATTAATGGATAAAGAAGAGGGTCTTCTTAACCTTATTTTGCGAGATGATAGAAGCAGTTGGCACGAATTCATAGAAGATTTCTTTGGTTGTTGTGAATCAGTGTTAGAGGAAAATGGTCTTCTTTTCATATTAGTGCCAGACGAGGAGCATCGGCTAAGTTCTGGTTTTATAAAAGAATATATATTTCCAGGAGAGTGCGTACCGTTATTAAGTAGAATAACAACGGCCATGGCTAGTGCATCAAGGCTTTGGTAA

Protein sequence

MQARNVLSLIEKGTRLFITRFLVVEKKKIKYNLFLRLREKLDLGLAGAYINGEFSLMDKEEGLLNLILRDDRSSWHEFIEDFFGCCESVLEENGLLFILVPDEEHRLSSGFIKEYIFPGECVPLLSRITTAMASASRLW

Homology

[Close]

BLAST of CmoCh19G003400 vs. ExPASy TrEMBL
Match: A0A397YI84 (Amino_oxidase domain-containing protein OS=Brassica campestris OX=3711 GN=BRARA_G00593 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 4.0e-19
Identity = 72/194 (37.11%), Postives = 98/194 (50.52%), Query Frame = 0


Query: 8   SLIEKGTRLFITRFL-------------------VVEKKKIKYNL-----------FLRL 67
           S+ EKG R   TRFL                     +   ++ NL           + ++
Sbjct: 368 SITEKGARFLFTRFLRHFISTGCVTVDEEGGTMFTFKGNDLRCNLESVIKIHSPHFYWKV 427

Query: 68  REKLDLGLAGAYINGEFSLMDKEEGLLNLILRDDRSSW---------------------- 127
             + DLGLA AYING+FS  DK+ GLLNLI+  DR ++                      
Sbjct: 428 MTQADLGLADAYINGDFSFADKDSGLLNLIM--DRITFELCDYRQLSDARKYDRIISCEM 487

Query: 128 -----HEFIEDFFGCCESVLEENG---LLFILVPDE---EHRLSSGFIKEYIFPGECVPL 139
                HEF++ FF  CE+ L E+G   L FI +P+E   E+RL+S FIKEYIFPG C+P 
Sbjct: 488 IEHVGHEFMDTFFSHCEAALAEDGIFVLQFISIPEERYNEYRLNSDFIKEYIFPGGCLPS 547

[Close]

BLAST of CmoCh19G003400 vs. ExPASy TrEMBL
Match: A0A6J1IB60 (uncharacterized protein LOC111473008 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111473008 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.5e-18
Identity = 67/131 (51.15%), Postives = 83/131 (63.36%), Query Frame = 0


Query: 22  LVVEKKKIKYNLFLRLREKLDLGLAGAYINGEFSLMDKEEGLLNLILRDDR--------S 81
           + + K+++KY      +   DLGL     + EF L D  E  L+   + DR        +
Sbjct: 238 ITLSKQQLKY----AQKRVQDLGLQD---HIEFLLCDYRE--LSSTTKYDRILSCGMIEA 297

Query: 82  SWHEFIEDFFGCCESVLEENGLL---FILVPDE---EHRLSSGFIKEYIFPGECVPLLSR 139
             HEF+EDFFG CESVLE+NGLL   FI +PDE   E+RLS+GFIKEYIF G C+P LSR
Sbjct: 298 VGHEFMEDFFGSCESVLEDNGLLVLQFISMPDESYKEYRLSAGFIKEYIFQGGCIPSLSR 357

[Close]

BLAST of CmoCh19G003400 vs. ExPASy TrEMBL
Match: A0A6J1IFX4 (uncharacterized protein LOC111472470 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472470 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.5e-18
Identity = 67/131 (51.15%), Postives = 83/131 (63.36%), Query Frame = 0


Query: 22  LVVEKKKIKYNLFLRLREKLDLGLAGAYINGEFSLMDKEEGLLNLILRDDR--------S 81
           + + K+++KY      +   DLGL     + EF L D  E  L+   + DR        +
Sbjct: 217 ITLSKQQLKY----AQKRVQDLGLQD---HIEFLLCDYRE--LSSTTKYDRILSCGMIEA 276

Query: 82  SWHEFIEDFFGCCESVLEENGLL---FILVPDE---EHRLSSGFIKEYIFPGECVPLLSR 139
             HEF+EDFFG CESVLE+NGLL   FI +PDE   E+RLS+GFIKEYIF G C+P LSR
Sbjct: 277 VGHEFMEDFFGSCESVLEDNGLLVLQFISMPDESYKEYRLSAGFIKEYIFQGGCIPSLSR 336

[Close]

BLAST of CmoCh19G003400 vs. ExPASy TrEMBL
Match: A0A6J1IA38 (uncharacterized protein LOC111473008 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111473008 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.5e-18
Identity = 67/131 (51.15%), Postives = 83/131 (63.36%), Query Frame = 0


Query: 22  LVVEKKKIKYNLFLRLREKLDLGLAGAYINGEFSLMDKEEGLLNLILRDDR--------S 81
           + + K+++KY      +   DLGL     + EF L D  E  L+   + DR        +
Sbjct: 217 ITLSKQQLKY----AQKRVQDLGLQD---HIEFLLCDYRE--LSSTTKYDRILSCGMIEA 276

Query: 82  SWHEFIEDFFGCCESVLEENGLL---FILVPDE---EHRLSSGFIKEYIFPGECVPLLSR 139
             HEF+EDFFG CESVLE+NGLL   FI +PDE   E+RLS+GFIKEYIF G C+P LSR
Sbjct: 277 VGHEFMEDFFGSCESVLEDNGLLVLQFISMPDESYKEYRLSAGFIKEYIFQGGCIPSLSR 336

[Close]

BLAST of CmoCh19G003400 vs. ExPASy TrEMBL
Match: A0A6J1ICH0 (uncharacterized protein LOC111472470 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472470 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.5e-18
Identity = 67/131 (51.15%), Postives = 83/131 (63.36%), Query Frame = 0


Query: 22  LVVEKKKIKYNLFLRLREKLDLGLAGAYINGEFSLMDKEEGLLNLILRDDR--------S 81
           + + K+++KY      +   DLGL     + EF L D  E  L+   + DR        +
Sbjct: 238 ITLSKQQLKY----AQKRVQDLGLQD---HIEFLLCDYRE--LSSTTKYDRILSCGMIEA 297

Query: 82  SWHEFIEDFFGCCESVLEENGLL---FILVPDE---EHRLSSGFIKEYIFPGECVPLLSR 139
             HEF+EDFFG CESVLE+NGLL   FI +PDE   E+RLS+GFIKEYIF G C+P LSR
Sbjct: 298 VGHEFMEDFFGSCESVLEDNGLLVLQFISMPDESYKEYRLSAGFIKEYIFQGGCIPSLSR 357

[Close]

BLAST of CmoCh19G003400 vs. TAIR 10
Match: AT3G23530.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 86.7 bits (213), Expect = 1.7e-17
Identity = 43/69 (62.32%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 76  HEFIEDFFGCCESVLEENGLL---FILVPDE---EHRLSSGFIKEYIFPGECVPLLSRIT 135
           HEF+E FF  CE+ L ENGL+   FI +P+E   E+RLSS FIKEYIFPG C+P L+R+T
Sbjct: 710 HEFMEMFFSRCEAALAENGLIVLQFISIPEERYNEYRLSSDFIKEYIFPGGCLPSLARVT 769

Query: 136 TAMASASRL 139
           TAM+S+SRL
Sbjct: 770 TAMSSSSRL 778

HSP 2 Score: 50.8 bits (120), Expect = 1.0e-06
Identity = 37/104 (35.58%), Postives = 51/104 (49.04%), Query Frame = 0


Query: 6   VLSLIEKGTRLFITRFL----------VVEKKKIKY--------------------NLFL 65
           V SL E G RLF+TRFL          ++E+    +                      + 
Sbjct: 433 VPSLTETGARLFVTRFLGQFISTGSVTILEEGGTMFTFGGKDSTCPLKSILKIHSPQFYW 492

Query: 66  RLREKLDLGLAGAYINGEFSLMDKEEGLLNLIL-----RDDRSS 75
           ++  + DLGLA AYING+FS +DKE GLLNLI+     RD +S+
Sbjct: 493 KVMTQADLGLADAYINGDFSFVDKESGLLNLIMILIANRDTKSN 536

[Close]

BLAST of CmoCh19G003400 vs. TAIR 10
Match: AT3G23470.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 84.0 bits (206), Expect = 1.1e-16
Identity = 40/69 (57.97%), Postives = 51/69 (73.91%), Query Frame = 0


Query: 76  HEFIEDFFGCCESVLEENG---LLFILVPD---EEHRLSSGFIKEYIFPGECVPLLSRIT 135
           HEF++ FF CCE  L ENG   L F  +P+   +E RL+SGFI EYIFPG C+P L+R+T
Sbjct: 302 HEFMDKFFSCCEDALAENGIFVLQFTAIPEALYDESRLTSGFITEYIFPGGCLPSLARVT 361

Query: 136 TAMASASRL 139
           +AMAS+SRL
Sbjct: 362 SAMASSSRL 370

HSP 2 Score: 52.0 bits (123), Expect = 4.5e-07
Identity = 37/103 (35.92%), Postives = 51/103 (49.51%), Query Frame = 0


Query: 6   VLSLIEKGTRLFITRFL----------VVEKKKIKY--------------------NLFL 65
           VLSL E G RLF+TRFL          ++E+    +                      + 
Sbjct: 22  VLSLTESGARLFVTRFLEQFISIGCVTILEEGGTMFIFGEKDSTCPLKSILKIHSPQFYW 81

Query: 66  RLREKLDLGLAGAYINGEFSLMDKEEGLLNLIL-----RDDRS 74
           ++  + DLGLA AYI+G+FS +DK+ GLLNLI+     RD RS
Sbjct: 82  KVMTQADLGLADAYISGDFSFVDKDSGLLNLIMILIANRDQRS 124

[Close]

BLAST of CmoCh19G003400 vs. TAIR 10
Match: AT3G23510.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 82.4 bits (202), Expect = 3.1e-16
Identity = 41/69 (59.42%), Postives = 53/69 (76.81%), Query Frame = 0


Query: 76  HEFIEDFFGCCESVLEENGLL---FILVPDE---EHRLSSGFIKEYIFPGECVPLLSRIT 135
           HEF+E FF  CE+ L E+GL+   FI  P+E   E+RLSS FIKEYIFPG CVP L+++T
Sbjct: 710 HEFMEMFFSRCEAALAEDGLMVLQFISTPEERYNEYRLSSDFIKEYIFPGACVPSLAKVT 769

Query: 136 TAMASASRL 139
           +AM+S+SRL
Sbjct: 770 SAMSSSSRL 778

HSP 2 Score: 50.8 bits (120), Expect = 1.0e-06
Identity = 37/104 (35.58%), Postives = 51/104 (49.04%), Query Frame = 0


Query: 6   VLSLIEKGTRLFITRFL----------VVEKKKIKY--------------------NLFL 65
           V SL E G RLF+TRFL          ++E+    +                      + 
Sbjct: 433 VPSLTETGARLFVTRFLGQFISTGSVTILEEGGTMFTFGGKDSTCPLKSILKIHSPQFYW 492

Query: 66  RLREKLDLGLAGAYINGEFSLMDKEEGLLNLIL-----RDDRSS 75
           ++  + DLGLA AYING+FS +DKE GLLNLI+     RD +S+
Sbjct: 493 KVMTQADLGLADAYINGDFSFVDKESGLLNLIMILIANRDTKSN 536

[Close]

BLAST of CmoCh19G003400 vs. TAIR 10
Match: AT3G23480.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 78.6 bits (192), Expect = 4.5e-15
Identity = 39/69 (56.52%), Postives = 51/69 (73.91%), Query Frame = 0


Query: 76  HEFIEDFFGCCESVLEENG---LLFILVPDE---EHRLSSGFIKEYIFPGECVPLLSRIT 135
           H+F+E FF  CE+ L E+G   L F  +P+E   E RL+SGFI EYIFPG C+P L+R+T
Sbjct: 300 HKFMETFFSHCEAALAEDGIFVLQFTAIPEELYDESRLTSGFITEYIFPGGCLPSLARVT 359

Query: 136 TAMASASRL 139
           +AMAS+SRL
Sbjct: 360 SAMASSSRL 368

HSP 2 Score: 47.8 bits (112), Expect = 8.5e-06
Identity = 34/92 (36.96%), Postives = 46/92 (50.00%), Query Frame = 0


Query: 6   VLSLIEKGTRLFITRFL----------VVE----------------KKKIKYN---LFLR 65
           V SL E G RLF+TRF           ++E                K  +K +    + +
Sbjct: 22  VFSLTEAGARLFVTRFFRQFISIGCVTILEGDTIFIFGENESTCPLKSILKIHSPQFYWK 81

Query: 66  LREKLDLGLAGAYINGEFSLMDKEEGLLNLIL 69
           +    DLGLA AYING+FS +DK+ GLLNLI+
Sbjct: 82  VMTLADLGLADAYINGDFSFVDKDSGLLNLIM 113

[Close]

BLAST of CmoCh19G003400 vs. TAIR 10
Match: AT3G23480.2 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 78.6 bits (192), Expect = 4.5e-15
Identity = 39/69 (56.52%), Postives = 51/69 (73.91%), Query Frame = 0


Query: 76  HEFIEDFFGCCESVLEENG---LLFILVPDE---EHRLSSGFIKEYIFPGECVPLLSRIT 135
           H+F+E FF  CE+ L E+G   L F  +P+E   E RL+SGFI EYIFPG C+P L+R+T
Sbjct: 279 HKFMETFFSHCEAALAEDGIFVLQFTAIPEELYDESRLTSGFITEYIFPGGCLPSLARVT 338

Query: 136 TAMASASRL 139
           +AMAS+SRL
Sbjct: 339 SAMASSSRL 347

HSP 2 Score: 47.8 bits (112), Expect = 8.5e-06
Identity = 34/92 (36.96%), Postives = 46/92 (50.00%), Query Frame = 0


Query: 6   VLSLIEKGTRLFITRFL----------VVE----------------KKKIKYN---LFLR 65
           V SL E G RLF+TRF           ++E                K  +K +    + +
Sbjct: 22  VFSLTEAGARLFVTRFFRQFISIGCVTILEGDTIFIFGENESTCPLKSILKIHSPQFYWK 81

Query: 66  LREKLDLGLAGAYINGEFSLMDKEEGLLNLIL 69
           +    DLGLA AYING+FS +DK+ GLLNLI+
Sbjct: 82  VMTLADLGLADAYINGDFSFVDKDSGLLNLIM 113

The following BLAST results are available for this feature:

BLAST of CmoCh19G003400 vs.


Match Name	E-value	Identity	Description

BLAST of CmoCh19G003400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A397YI84	4.0e-19	37.11	Amino_oxidase domain-containing protein OS=Brassica campestris OX=3711 GN=BRARA_...	[more]
A0A6J1IB60	1.5e-18	51.15	uncharacterized protein LOC111473008 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1IFX4	1.5e-18	51.15	uncharacterized protein LOC111472470 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1IA38	1.5e-18	51.15	uncharacterized protein LOC111473008 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1ICH0	1.5e-18	51.15	uncharacterized protein LOC111472470 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of CmoCh19G003400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23530.1	1.7e-17	62.32	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23470.1	1.1e-16	57.97	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23510.1	3.1e-16	59.42	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23480.1	4.5e-15	56.52	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23480.2	4.5e-15	56.52	Cyclopropane-fatty-acyl-phospholipid synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 63..138 e-value: 5.0E-8 score: 34.4
None	No IPR available	PANTHER	PTHR43675:SF20	METHYLTRANSFERASE, PUTATIVE-RELATED	coord: 33..68 coord: 75..138
IPR026669	Arsenite methyltransferase-like	PANTHER	PTHR43675	ARSENITE METHYLTRANSFERASE	coord: 75..138
IPR026669	Arsenite methyltransferase-like	PANTHER	PTHR43675	ARSENITE METHYLTRANSFERASE	coord: 33..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh19G003400.1	CmoCh19G003400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008168	methyltransferase activity