CmoCh19G000770 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh19G000770
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptioninhibitor of trypsin and hageman factor-like
LocationCmo_Chr19: 423656 .. 424198 (-)
RNA-Seq ExpressionCmoCh19G000770
SyntenyCmoCh19G000770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTACCCTTCAGCAAATACATACACATCCATCAACTTCATCCAAAAATGAATTATTTTTGTCCGCATGGTGGGTTTCTCACCATTTCTCGTTTTAGTACTTCTATCCTATCCTCGAGATTCGTTTTGTGATGAAACTATACGATTTTTAGAATAGTTTCAATTAAGTTTTGAAAATCAAGTTGTTTATACGATCATTGATTTGTTTATTTGATTTGGTTTATCAGGAAAGAATAGGTGGCCCGAACTTGTTGGAGTAGAAGCAAAGATTGCAGAGAAAATCATCAAGAAAGAAAATCCCGCTGTAAGGATGGTTCAGATTTTCTTAGCGGGAAGTTCTGTGACGTACAATCTTAGCTACGGTCGAGTTCGATTGTTTGTTAATATAAATGGTGTAGTTGTTAGCATACCAGGGATTGGTTAAAAAAAAAATATATCCAAAAGATGTTGATGGATGCATATGTATTGTATCGTCTCTATCTTTTTTATTATAATAAAATTGCTACAACATGACTTAATTACGAAATGCGATTTTTTTTTTAAAA

mRNA sequence

ACTACCCTTCAGCAAATACATACACATCCATCAACTTCATCCAAAAATGAATTATTTTTGTCCGCATGGAAAGAATAGGTGGCCCGAACTTGTTGGAGTAGAAGCAAAGATTGCAGAGAAAATCATCAAGAAAGAAAATCCCGCTGTAAGGATGGTTCAGATTTTCTTAGCGGGAAGTTCTGTGACGTACAATCTTAGCTACGGTCGAGTTCGATTGTTTGTTAATATAAATGGTGTAGTTGTTAGCATACCAGGGATTGGTTAAAAAAAAAATATATCCAAAAGATGTTGATGGATGCATATGTATTGTATCGTCTCTATCTTTTTTATTATAATAAAATTGCTACAACATGACTTAATTACGAAATGCGATTTTTTTTTTAAAA

Coding sequence (CDS)

ATGAATTATTTTTGTCCGCATGGAAAGAATAGGTGGCCCGAACTTGTTGGAGTAGAAGCAAAGATTGCAGAGAAAATCATCAAGAAAGAAAATCCCGCTGTAAGGATGGTTCAGATTTTCTTAGCGGGAAGTTCTGTGACGTACAATCTTAGCTACGGTCGAGTTCGATTGTTTGTTAATATAAATGGTGTAGTTGTTAGCATACCAGGGATTGGTTAA

Protein sequence

MNYFCPHGKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNINGVVVSIPGIG
Homology
BLAST of CmoCh19G000770 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 2.0e-11
Identity = 34/68 (50.00%), Postives = 46/68 (67.65%), Query Frame = 0

Query: 2   NYFCPHGKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNI 61
           N  C  GK  WPEL+GV  K+A++II+KENP++  + I L+GS +T +    RVRLF NI
Sbjct: 42  NLMC-EGKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNI 101

Query: 62  NGVVVSIP 70
            G VV +P
Sbjct: 102 LGFVVQMP 108

BLAST of CmoCh19G000770 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 2.0e-11
Identity = 36/65 (55.38%), Postives = 44/65 (67.69%), Query Frame = 0

Query: 8   GKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNINGVVVS 67
           GK RWPEL+GV  K+A+ II+KEN  +  V I L GS VT ++   RVRLF NI G VV 
Sbjct: 43  GKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVD 102

Query: 68  IPGIG 73
           IP +G
Sbjct: 103 IPVVG 107

BLAST of CmoCh19G000770 vs. ExPASy Swiss-Prot
Match: P08454 (Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 2.0e-11
Identity = 36/65 (55.38%), Postives = 44/65 (67.69%), Query Frame = 0

Query: 7   HGKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNINGVVV 66
           +GK RWPEL+GV  K+A+ II+KEN  +  VQI L GS VT +    RVRLF NI G VV
Sbjct: 42  NGKQRWPELIGVPTKLAKGIIEKENSLITNVQILLNGSPVTMDYRCNRVRLFDNILGDVV 101

Query: 67  SIPGI 72
            IP +
Sbjct: 102 QIPRV 106

BLAST of CmoCh19G000770 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.4e-11
Identity = 35/70 (50.00%), Postives = 46/70 (65.71%), Query Frame = 0

Query: 4   FCPH-GKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNIN 63
           FCP   K  WPEL+G  AK A++II+KENP +  V+  L GS+ T +L   RVRLFVN+ 
Sbjct: 50  FCPGVTKESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLL 109

Query: 64  GVVVSIPGIG 73
            +VV  P +G
Sbjct: 110 DIVVQTPKVG 119

BLAST of CmoCh19G000770 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 9.9e-11
Identity = 35/64 (54.69%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 8   GKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNINGVVVS 67
           GK  WPEL+GV A  A+ II+KENP++  + I L GS VT +    RVRLFVNI G VV 
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 68  IPGI 72
           IP +
Sbjct: 107 IPRV 110

BLAST of CmoCh19G000770 vs. ExPASy TrEMBL
Match: A0A6J1HFK9 (inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LOC111463862 PE=3 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.9e-17
Identity = 49/71 (69.01%), Postives = 55/71 (77.46%), Query Frame = 0

Query: 4  FCPHGKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAG--SSVTYNLSYGRVRLFVNI 63
          FCP GKNRWPELVG+EA+IAEKII+KENP V +VQIFL G  S +T      RVRL VNI
Sbjct: 4  FCPPGKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNI 63

Query: 64 NGVVVSIPGIG 73
          NGV+V IP IG
Sbjct: 64 NGVIVGIPSIG 74

BLAST of CmoCh19G000770 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.1e-11
Identity = 37/68 (54.41%), Postives = 49/68 (72.06%), Query Frame = 0

Query: 5  CPHGKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNINGV 64
          CP GKN+WPELVGV+A  A+ IIKK+NP V  V + LAGS  T ++   RV +FVNI+ +
Sbjct: 5  CPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHDL 64

Query: 65 VVSIPGIG 73
          VV +P +G
Sbjct: 65 VVEVPKVG 72

BLAST of CmoCh19G000770 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.7e-11
Identity = 37/68 (54.41%), Postives = 48/68 (70.59%), Query Frame = 0

Query: 5  CPHGKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNINGV 64
          CP GKN+WPELVG++A  AE II+K+NP+V  V + LAG+  T ++   RV LFVNI   
Sbjct: 5  CPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVNIEEK 64

Query: 65 VVSIPGIG 73
          VV IP +G
Sbjct: 65 VVEIPKVG 72

BLAST of CmoCh19G000770 vs. ExPASy TrEMBL
Match: E9JGS6 (Serine proteinase inhibitor 1a (Fragment) OS=Solanum nigrum OX=4112 GN=SPI1a PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.8e-11
Identity = 38/64 (59.38%), Postives = 47/64 (73.44%), Query Frame = 0

Query: 9  KNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNINGVVVSI 68
          K RWPELVGV AK+A+ II+KENP +  V I L GS VT++ S  RVR+F+NI G VV I
Sbjct: 1  KQRWPELVGVPAKLAKGIIEKENPFITHVPILLIGSPVTFDFSCNRVRIFINIFGNVVDI 60

Query: 69 PGIG 73
          P +G
Sbjct: 61 PIVG 64

BLAST of CmoCh19G000770 vs. ExPASy TrEMBL
Match: Q4FE26 (Proteinase inhibitor 1 PPI3B2 OS=Solanum tuberosum OX=4113 GN=PPI3B2 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.1e-10
Identity = 37/65 (56.92%), Postives = 47/65 (72.31%), Query Frame = 0

Query: 8   GKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNINGVVVS 67
           GK +WPEL+GV  K+A++II+KEN  +  VQI L GS VT + S  RVRLF NI GVVV 
Sbjct: 43  GKLQWPELIGVPTKLAKEIIEKENSLITNVQILLNGSPVTLDFSCNRVRLFDNILGVVVD 102

Query: 68  IPGIG 73
           +P +G
Sbjct: 103 MPVVG 107

BLAST of CmoCh19G000770 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 54.3 bits (129), Expect = 4.7e-08
Identity = 31/72 (43.06%), Postives = 40/72 (55.56%), Query Frame = 0

Query: 1  MNYFCPHGKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVN 60
          M+  CP  KN WPEL G     A  +I++ENP V    + L GS VT +    RVR+FV+
Sbjct: 1  MSTECPR-KNSWPELTGTNGDYAAVVIERENPTVN-AAVILDGSPVTADFRCDRVRVFVD 60

Query: 61 INGVVVSIPGIG 73
           N +VV  P  G
Sbjct: 61 GNRIVVKTPKSG 70

BLAST of CmoCh19G000770 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 51.2 bits (121), Expect = 4.0e-07
Identity = 28/65 (43.08%), Postives = 43/65 (66.15%), Query Frame = 0

Query: 8  GKNRWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNINGVVVS 67
          GK+ WPEL+G + + A+++I++ENP ++ V I L G+ V       RV ++VN  G+VV 
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWVNDCGIVVQ 95

Query: 68 IPGIG 73
          IP IG
Sbjct: 96 IPIIG 99

BLAST of CmoCh19G000770 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 43.9 bits (102), Expect = 6.4e-05
Identity = 26/69 (37.68%), Postives = 36/69 (52.17%), Query Frame = 0

Query: 5  CPHGKN-RWPELVGVEAKIAEKIIKKENPAVRMVQIFLAGSSVTYNLSYGRVRLFVNING 64
          C  GK  RWPELVG   ++A+  I++ENP V  + +   G     N    RV +++  NG
Sbjct: 18 CAIGKKYRWPELVGKNGQLAKMTIERENPNVLAI-VLRYGEKRIENFCCNRVFVYLGSNG 77

Query: 65 VVVSIPGIG 73
           V   P IG
Sbjct: 78 QVADAPMIG 85

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P051182.0e-1150.00Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=... [more]
Q007832.0e-1155.38Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1[more]
P084542.0e-1155.38Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2[more]
P200764.4e-1150.00Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ... [more]
P162319.9e-1154.69Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HFK91.9e-1769.01inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0KME72.1e-1154.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1[more]
A0A0M3SAG82.7e-1154.41Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1[more]
E9JGS67.8e-1159.38Serine proteinase inhibitor 1a (Fragment) OS=Solanum nigrum OX=4112 GN=SPI1a PE=... [more]
Q4FE265.1e-1056.92Proteinase inhibitor 1 PPI3B2 OS=Solanum tuberosum OX=4113 GN=PPI3B2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38870.14.7e-0843.06Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT5G43580.14.0e-0743.08Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT3G46860.16.4e-0537.68Serine protease inhibitor, potato inhibitor I-type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 11..24
score: 55.59
coord: 49..59
score: 47.43
coord: 37..48
score: 41.67
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 9..72
e-value: 3.1E-20
score: 72.6
IPR000864Proteinase inhibitor I13, potato inhibitor IPANTHERPTHR33091PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 5..72
IPR000864Proteinase inhibitor I13, potato inhibitor IPROSITEPS00285POTATO_INHIBITORcoord: 12..23
NoneNo IPR availableGENE3D3.30.10.10Trypsin Inhibitor V, subunit Acoord: 5..72
e-value: 3.0E-21
score: 77.4
NoneNo IPR availablePANTHERPTHR33091:SF83ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1coord: 5..72
IPR036354Proteinase inhibitor I13, potato inhibitor I superfamilySUPERFAMILY54654CI-2 family of serine protease inhibitorscoord: 5..72

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh19G000770.1CmoCh19G000770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010466 negative regulation of peptidase activity
biological_process GO:0009611 response to wounding
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity