CmoCh19G000650 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh19G000650
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	inhibitor of trypsin and hageman factor-like
Location	Cmo_Chr19: 374825 .. 375281 (-)
RNA-Seq Expression	CmoCh19G000650
Synteny	CmoCh19G000650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGATTTTTGTCCGGGTGGGTTTCTCACCATTTCTCGTTTTAGTACTTCTATCCTCGAGATTTGTTTTGTGATGAAACTATACGATTTTTAGAATAGTTTCAATTAAGTTTTGAAAATCAAGTTGATTTTACGATCATTGATTTGTTTAGTTGATTTGGTTTTTCAGGAAAGAATAGGTGGCCCGAACTTGTTGGAATAGAAGCAAGGATTGCAGAGAAAATCATCCAAAAAGAAAATCCCAACGTAGATATTGTTCAGATTTTCTTATTTGGAAGTAAAAGTGGTATAACGATGGAATTTTGTTGCAGTCGAGTTCGATTGCTTGTTAATATAAATGGTGTAATTGTTGGTATACCATCGATTGGGTAGAAAAAACTATCAAAAAAATGGTGATGGATGCACATGTATTGTAACAACCCTTTTCTTACTTCAATAAAGTTTTTCTTACTTCA

mRNA sequence

ATGGGAAAGAATAGGTGGCCCGAACTTGTTGGAATAGAAGCAAGGATTGCAGAGAAAATCATCCAAAAAGAAAATCCCAACGTAGATATTGTTCAGATTTTCTTATTTGGAAGTAAAAGTGGTATAACGATGGAATTTTGTTGCAGTCGAGTTCGATTGCTTGTTAATATAAATGGTGTAATTGTTGGTATACCATCGATTGGGTAGAAAAAACTATCAAAAAAATGGTGATGGATGCACATGTATTGTAACAACCCTTTTCTTACTTCAATAAAGTTTTTCTTACTTCA

Coding sequence (CDS)

ATGGGAAAGAATAGGTGGCCCGAACTTGTTGGAATAGAAGCAAGGATTGCAGAGAAAATCATCCAAAAAGAAAATCCCAACGTAGATATTGTTCAGATTTTCTTATTTGGAAGTAAAAGTGGTATAACGATGGAATTTTGTTGCAGTCGAGTTCGATTGCTTGTTAATATAAATGGTGTAATTGTTGGTATACCATCGATTGGGTAG

Protein sequence

MGKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVIVGIPSIG

Homology

[Close]

BLAST of CmoCh19G000650 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.2e-10
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 0


Query: 2   GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
           GK  WPEL+G+  ++A++II+KENP++  + I L GS   IT+++ C RVRL  NI G +
Sbjct: 47  GKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSP--ITLDYLCDRVRLFDNILGFV 106

Query: 62  VGIPSI 68
           V +P +
Sbjct: 107 VQMPVV 110

[Close]

BLAST of CmoCh19G000650 vs. ExPASy Swiss-Prot
Match: P08454 (Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 1.2e-10
Identity = 33/66 (50.00%), Postives = 46/66 (69.70%), Query Frame = 0


Query: 2   GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
           GK RWPEL+G+  ++A+ II+KEN  +  VQI L GS   +TM++ C+RVRL  NI G +
Sbjct: 43  GKQRWPELIGVPTKLAKGIIEKENSLITNVQILLNGSP--VTMDYRCNRVRLFDNILGDV 102

Query: 62  VGIPSI 68
           V IP +
Sbjct: 103 VQIPRV 106

[Close]

BLAST of CmoCh19G000650 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.7e-10
Identity = 33/66 (50.00%), Postives = 44/66 (66.67%), Query Frame = 0


Query: 2   GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
           GK  WPEL+G+ A  A+ II+KENP++  + I L GS   +T +F C RVRL VNI G +
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSP--VTKDFRCDRVRLFVNILGDV 106

Query: 62  VGIPSI 68
           V IP +
Sbjct: 107 VQIPRV 110

[Close]

BLAST of CmoCh19G000650 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 6.0e-10
Identity = 32/67 (47.76%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 2   GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
           GK RWPEL+G+  ++A+ II+KEN  +  V I L GS   +T++  C RVRL  NI G +
Sbjct: 43  GKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSP--VTLDIRCDRVRLFDNILGYV 102

Query: 62  VGIPSIG 69
           V IP +G
Sbjct: 103 VDIPVVG 107

[Close]

BLAST of CmoCh19G000650 vs. ExPASy Swiss-Prot
Match: P01052 (Chymotrypsin inhibitor I, A, B and C subunits OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 3.0e-09
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 0


Query: 2  GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
          GK +WPEL+G+  ++A++II+K+N  +  V I L GS   +TM+F C+RVRL  +I G +
Sbjct: 7  GKLQWPELIGVPTKLAKEIIEKQNSLISNVHILLNGSP--VTMDFRCNRVRLFDDILGSV 66

Query: 62 VGIPSI 68
          V IP +
Sbjct: 67 VQIPRV 70

[Close]

BLAST of CmoCh19G000650 vs. ExPASy TrEMBL
Match: A0A6J1HFK9 (inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LOC111463862 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.1e-29
Identity = 67/67 (100.00%), Postives = 67/67 (100.00%), Query Frame = 0


Query: 2  GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
          GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI
Sbjct: 8  GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 67

Query: 62 VGIPSIG 69
          VGIPSIG
Sbjct: 68 VGIPSIG 74

[Close]

BLAST of CmoCh19G000650 vs. ExPASy TrEMBL
Match: E9JGS6 (Serine proteinase inhibitor 1a (Fragment) OS=Solanum nigrum OX=4112 GN=SPI1a PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-09
Identity = 34/66 (51.52%), Postives = 47/66 (71.21%), Query Frame = 0


Query: 3  KNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVIV 62
          K RWPELVG+ A++A+ II+KENP +  V I L GS   +T +F C+RVR+ +NI G +V
Sbjct: 1  KQRWPELVGVPAKLAKGIIEKENPFITHVPILLIGSP--VTFDFSCNRVRIFINIFGNVV 60

Query: 63 GIPSIG 69
           IP +G
Sbjct: 61 DIPIVG 64

[Close]

BLAST of CmoCh19G000650 vs. ExPASy TrEMBL
Match: W9R3U3 (Glu S.griseus protease inhibitor OS=Morus notabilis OX=981085 GN=L484_017423 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.8e-09
Identity = 32/67 (47.76%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 2  GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
          GKN WPELVG++  +AEKII+KENP VD +   +    S +T ++ C RVR+ VN +G++
Sbjct: 7  GKNSWPELVGVQGEVAEKIIEKENPAVDAI---IVTEGSVVTADYRCDRVRVWVNNHGIV 66

Query: 62 VGIPSIG 69
            +P+IG
Sbjct: 67 YKVPTIG 70

[Close]

BLAST of CmoCh19G000650 vs. ExPASy TrEMBL
Match: A0A3Q7J2Q4 (Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247257 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.4e-09
Identity = 33/66 (50.00%), Postives = 48/66 (72.73%), Query Frame = 0


Query: 2   GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
           GK  WPEL+G+ A++A++II+KENP++  + I L GS   +TM+F C RVRL  NI G +
Sbjct: 47  GKQFWPELIGVPAKLAKEIIEKENPSISDIPILLNGSP--VTMDFRCDRVRLFDNILGYV 106

Query: 62  VGIPSI 68
           V IP++
Sbjct: 107 VQIPTV 110

[Close]

BLAST of CmoCh19G000650 vs. ExPASy TrEMBL
Match: A0A6N2AU19 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023457 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.4e-09
Identity = 34/67 (50.75%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 2   GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
           GK  WPELVG+  ++A+KII+KENP++  + I   GS   +TM+F C+RVRL  NI G +
Sbjct: 47  GKQFWPELVGVPTKLAKKIIEKENPSISDIPILFNGSP--LTMDFRCNRVRLFDNILGYV 106

Query: 62  VGIPSIG 69
           V IP +G
Sbjct: 107 VQIPVVG 111

[Close]

BLAST of CmoCh19G000650 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 53.9 bits (128), Expect = 5.8e-08
Identity = 27/66 (40.91%), Postives = 39/66 (59.09%), Query Frame = 0


Query: 3  KNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVIV 62
          K RWPELVG   ++A+  I++ENPNV +  +  +G K      FCC+RV + +  NG + 
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNV-LAIVLRYGEKR--IENFCCNRVFVYLGSNGQVA 82

Query: 63 GIPSIG 69
            P IG
Sbjct: 83 DAPMIG 85

[Close]

BLAST of CmoCh19G000650 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.4 bits (124), Expect = 1.7e-07
Identity = 29/67 (43.28%), Postives = 43/67 (64.18%), Query Frame = 0


Query: 2  GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 61
          GK+ WPEL+G +   A+++I++ENP +  V I L G+   +   F CSRV + VN  G++
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAV-IILDGTV--VPEIFICSRVYVWVNDCGIV 95

Query: 62 VGIPSIG 69
          V IP IG
Sbjct: 96 VQIPIIG 99

[Close]

BLAST of CmoCh19G000650 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 51.6 bits (122), Expect = 2.9e-07
Identity = 26/66 (39.39%), Postives = 36/66 (54.55%), Query Frame = 0


Query: 3  KNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVIV 62
          KN WPEL G     A  +I++ENP V+   I      S +T +F C RVR+ V+ N ++V
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVIL---DGSPVTADFRCDRVRVFVDGNRIVV 67

Query: 63 GIPSIG 69
            P  G
Sbjct: 68 KTPKSG 70

[Close]

BLAST of CmoCh19G000650 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 44.7 bits (104), Expect = 3.5e-05
Identity = 22/68 (32.35%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 1  MGKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGV 60
          +GKN WPEL+G     A  +I+ EN ++++V +      + +T +  C RVR+ V+   +
Sbjct: 9  IGKNSWPELLGTNGDYAASVIKGENSSLNVVVV---SDGNYVTEDLSCYRVRVWVDEIRI 68

Query: 61 IVGIPSIG 69
          +V  P+ G
Sbjct: 69 VVRNPTAG 73

[Close]

BLAST of CmoCh19G000650 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 44.7 bits (104), Expect = 3.5e-05
Identity = 22/68 (32.35%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 1  MGKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGV 60
          +GKN WPEL+G     A  +I+ EN ++++V +      + +T +  C RVR+ V+   +
Sbjct: 24 IGKNSWPELLGTNGDYAASVIKGENSSLNVVVV---SDGNYVTEDLSCYRVRVWVDEIRI 83

Query: 61 IVGIPSIG 69
          +V  P+ G
Sbjct: 84 VVRNPTAG 88

The following BLAST results are available for this feature:

BLAST of CmoCh19G000650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05118	1.2e-10	45.45	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]
P08454	1.2e-10	50.00	Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2	[more]
P16231	2.7e-10	50.00	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
Q00783	6.0e-10	47.76	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
P01052	3.0e-09	45.45	Chymotrypsin inhibitor I, A, B and C subunits OS=Solanum tuberosum OX=4113 PE=1 ...	[more]

BLAST of CmoCh19G000650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HFK9	2.1e-29	100.00	inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
E9JGS6	1.1e-09	51.52	Serine proteinase inhibitor 1a (Fragment) OS=Solanum nigrum OX=4112 GN=SPI1a PE=...	[more]
W9R3U3	1.8e-09	47.76	Glu S.griseus protease inhibitor OS=Morus notabilis OX=981085 GN=L484_017423 PE=...	[more]
A0A3Q7J2Q4	2.4e-09	50.00	Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247257 PE=3 SV=1	[more]
A0A6N2AU19	2.4e-09	50.75	Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023457 PE=3 SV=1	[more]

BLAST of CmoCh19G000650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46860.1	5.8e-08	40.91	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	1.7e-07	43.28	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38870.1	2.9e-07	39.39	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.1	3.5e-05	32.35	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.2	3.5e-05	32.35	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..68 e-value: 7.3E-22 score: 79.3
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 2..68
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 3..68 e-value: 5.8E-20 score: 71.7
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 2..68
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 6..17
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 2..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh19G000650.1	CmoCh19G000650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity