CmoCh19G000130 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh19G000130
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionbeta-carotene isomerase D27, chloroplastic-like isoform X3
LocationCmo_Chr19: 86956 .. 90875 (+)
RNA-Seq ExpressionCmoCh19G000130
SyntenyCmoCh19G000130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCCTGAAATCTCCCTCCCTCCCTCCCTCCAAAAATACAAGAAAAACGCCCTTGAGTCCCAAGAGAAAGAAATGGAAGTAAGAGTTGGTGAGGTAAGGAGAGTGATTTTGGGAAGTGGGACAAACGTGAGGAGAAGCAACAAAACGAAGAAGATGAGAAGAAAAAGCATTGCCTGTGTAGCAGTGCTTACAAGAGCAGCAGAGAGTGGAGCAACAGAAGAAACAAGAAAACCAAACACTGTTTATACAGACAACTGGTTTGAGAAAATAGCCATCCACCATGTATCTCAGGCAGTACAAGCAACCTCAGGTGATCTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTGTTTTGAAGATTTGGAAAATAGAATTAATTATGATGTATATTCAGGTTGGAGAAGCAATAAGAGTGGATATGAAAGCTTGGTGGAAGTGGCAACAATGGCGTCAAGAAACTACAACCAGATCAAACAAAGAGAGGTTATCATTCAGGCGCTTGGAAGAGCATTTCCAGCTCCAATCCTCTCTTTGGTAAATTTCATTCATTCATTCATTCAATATCATTTACTACTCTTTTGACTTTCAATGTATTTATGATATAAATGCATATATCATGCATATATGTAAAATACTATCACCACACATATCATTAAAGATTTTACCAATACCAATTTCAAGACTACAATCATTTTACTCTCACCACTCCCTCAAATATTATTGCTAAATGTAATTTCAAAAGTCATATATTTATTTTTCTAGGCGGCTCTAAGTTATTTATTTACCTATGATATTAGGTGATAAAAGCAATAAATGTTTCCACATCAATAGAAGTTCATTATTAAATAATTTGATGAATTTCATTTAGGCCAAATTTCAATACTTTGTCTTCCTTATTTGGTCGGTCTCCCTTCTTCTTCCCATTTGTTTGTTTAAGTATTGACATAATTAAGTTTTTTTACCATAACCTATAAGCTAGAAGTTTCAAGTTGATTGTGGTATCAGAGCATGAGGTTATGTGTCCTGTGTTTGAACCCTATAATATAATTTTCTGATTTCGAACTTAAATTCGTAGGATCCAACACATTTTGGGGGATATAGCTAGGTTTCATTGCCTAGGTTAGAACCTACCTGGAACTATACCCTTTTCTAATTTTTCTTTCCATTAATCATGGGGTATAATAGTTATTTGATTGGCATGTCATTATATGGAAATAGTATCAGCAAATGATCACATTTTATATTATATTTCTAATTATCAGCAATTATTCGATTAAAGAAGTATTAAGACTGTACTTTTACTATTCTCGATCCATTTCCAATTTCGCCTTTTACTATTAAAACCCCACCCCTCTCAATCGTATGTCCCTAAAAAATATAGTGGTGAACAAAGTGCAGATAAGAACATTGCTACCTCAATCCAAATTCGCAAGGGAATATTTTGCTGCATTCACCACTGTCTTCTTTGCTTGGTTAGTTGGACCCTGCGAGGTAAGATGTTGTAAATAAATTTAGCCAAGGACATAAATTGGGAATTAACATCAATCCATGGACATAATTGGGAATTAACGGCTGCTTGTATACATACACATTTGGGTAAAGCACATATTATATTATCGACCAAAAAGTCAAAAGTTTGAATTCCTACTCCAAAGAGAGAGATGGTTCTGGGATCTGTCACAGGGCTTAGGATCGATGTCAAAAAAAATTTCAGAATATAGAGTATTTAAACATTCAACAAACAAGGTCAGAATAGAGATAGATTCCAGCTAATGGTGGACACAAGAACTTCAACTTTAGCACTAAATTCAATATAATAATATGATTTTCAATGGCCAAGTTGATAAACCTGCAGTGTCTGAGGCACTAGTCCCTTCTAGACTTGTCCTGTCCCTACAAATGAAAGAAAGGTTTTGTGACGTAAGATAGATAAACCTATTTTTTAAGAAACTATCTTCTCTTCATGCTTACTCTCTTTGGCCTTTCACTATCTAGGCATTCCTTAAGAACTCAGATTAAACACAATTGATATGTCTAGTTTGTTGGGGAGGGAGTGATTGAAAAAGATTTTTAAGAGAAACGACTAAACAATGCAGGAGCCATTCCCTACGCACACACAAATGGAAGAAGCAAGTCTCCTTCTCAATTACTAATTTAGTAACATATGATTCTTACAGGTGAAGGAATCGGACTTCAAAGGAAAAAGAGAAAAAAATGTGGTTCAAATCCACAAGTGCAGGTAATTCTTTCTCGCATCAGAGTTCTATAATTAAGGCAATGTTCGACCTACTTGAGACAATTTCCACGCCCACAGAACCTTCTGTCTGTAATTATTATGCTCATTCTAAACTCTCAGTACTTAATCACAGTAAGTCATGTCCATCATCCACATCATTGAAATTTTGAAATTATCGTTGTCAAAATAACACTCAGCAAATATATATTGTGCCTAAACATATCCCATTTATTTGCAGGTTCCTAGAGCAAACTAACTGTGCTGGAATGTGCATTAATCTATGCAAGTTTCCATGTCAGGATTTCATCAAGGATTCTTTGGGAATGCCAGTCAGCATGGTCCCCAGTAAGTCTCCTCTCACTAAGCTATAGAACCACCGAACAATTGCGGCAACCATATACAGTTTTCCCAAAAGAAAAACAAAATTTCAAATTCACATAGACTGCAGCACAAGGCAATTCTAGACACTAGTTCAAGCTGTCTAACTATAACTGAAGGCCATATTTCCTTTTTCTCGTATCCATAGTGTTCAGGTTTGTTTGCACACACCACTACTATTCTTACAAGATAATTGTCTGACTCTATTTAGTTTGGTTGCCAAAAAAAAAAAAAAAGAAATCATAGGATATTAAACAATTAAGCAGGTGAGTACAATGGCTAGAGCTCGTTCTCTCTAAGTTTTTTCCTATTTTCATGGGCTCTTTTTTATCACAAAACTAAATCATGGTGGTTAACTCAAAAGGCCATTATAAAAACTTCATATCCACATATAGAACATTAGAATTAAATTATTTCAAGACCTCTATATGGTTTAGATGAGAGGACACTAAATAAAGTAAATCTGCTATAGCACAATGACTGGTAGATCCATGAGTAAAAGTGAAATTACTATTAAAGCAACATTACCAGCACCAAATGGTGGAAAGGATCATGGTTGTCAATGTGAATGGAGGGTCCCATGCATGAACCATTTTGGGTACAAAAGAACTGCAGATAGGCCCATCCAAGCTAAGTGGGATTTCTAACCTGCAGCTTAATTTTGCAATATGGTTATAATCCAAGAGTTAGAAGCTGAATTCCCATCGTTAATTAATCATCAAGCTGGATTTCCCACTGAGCCAGGTATCGCTCTTGGTCATCATATACATCAATCATCACAGGTGTAAAAAATGCAACCAAGCAAAGGCATTTTTCTTAGGCGTTAATTATGCACCATGATGTAACTAATAGCTGGGAACCACTAGTATTAATGTCAAAGACCAAGAAACACGTCAATAAGCAAGGGGAATCTGGGAGCAAATGGGGGCGGCAAGAAGTATTTAAAAATATGATTAAAATAAGTGTCTTTCTTTGCAGATTTTGATGACATGAGCTGTGAGATGATATTTGGAAAGGATCCACCGGCTGCAGTCGATGATCCAGCGCTTAAGCAACCATGTTACAAACTATGTATTAACCTTACACGAGTCTTCTTCTTCTTTTCTTATTATTACCCTTTACTTCTTTACCTGCAAGTGGAGAAGTCGTATGTTATAATTCACTGGGCAAGGTAGCAGGACTACAGCAGGTCCAAACAAGATGTTTAGGGGGATGGTAATATAAGTTAAAAATGAATTCTAATCTTATTCGTTTACTTTTTGCAGGCAAAACAAAAGAAAAACATACGACAAACTGCTTCATTTAG

mRNA sequence

AAGCCTGAAATCTCCCTCCCTCCCTCCCTCCAAAAATACAAGAAAAACGCCCTTGAGTCCCAAGAGAAAGAAATGGAAGTAAGAGTTGGTGAGGTAAGGAGAGTGATTTTGGGAAGTGGGACAAACGTGAGGAGAAGCAACAAAACGAAGAAGATGAGAAGAAAAAGCATTGCCTGTGTAGCAGTGCTTACAAGAGCAGCAGAGAGTGGAGCAACAGAAGAAACAAGAAAACCAAACACTGTTTATACAGACAACTGGTTTGAGAAAATAGCCATCCACCATGTATCTCAGGCAGTACAAGCAACCTCAGGTTGGAGAAGCAATAAGAGTGGATATGAAAGCTTGGTGGAAGTGGCAACAATGGCGTCAAGAAACTACAACCAGATCAAACAAAGAGAGGTTATCATTCAGGCGCTTGGAAGAGCATTTCCAGCTCCAATCCTCTCTTTGATAAGAACATTGCTACCTCAATCCAAATTCGCAAGGGAATATTTTGCTGCATTCACCACTGTCTTCTTTGCTTGGTTAGTTGGACCCTGCGAGGTGAAGGAATCGGACTTCAAAGGAAAAAGAGAAAAAAATGTGGTTCAAATCCACAAGTGCAGGTTCCTAGAGCAAACTAACTGTGCTGGAATGTGCATTAATCTATGCAAGTTTCCATGTCAGGATTTCATCAAGGATTCTTTGGGAATGCCAGTCAGCATGGTCCCCAATTTTGATGACATGAGCTGTGAGATGATATTTGGAAAGGATCCACCGGCTGCAGTCGATGATCCAGCGCTTAAGCAACCATGTTACAAACTATGCAAAACAAAAGAAAAACATACGACAAACTGCTTCATTTAG

Coding sequence (CDS)

ATGGAAGTAAGAGTTGGTGAGGTAAGGAGAGTGATTTTGGGAAGTGGGACAAACGTGAGGAGAAGCAACAAAACGAAGAAGATGAGAAGAAAAAGCATTGCCTGTGTAGCAGTGCTTACAAGAGCAGCAGAGAGTGGAGCAACAGAAGAAACAAGAAAACCAAACACTGTTTATACAGACAACTGGTTTGAGAAAATAGCCATCCACCATGTATCTCAGGCAGTACAAGCAACCTCAGGTTGGAGAAGCAATAAGAGTGGATATGAAAGCTTGGTGGAAGTGGCAACAATGGCGTCAAGAAACTACAACCAGATCAAACAAAGAGAGGTTATCATTCAGGCGCTTGGAAGAGCATTTCCAGCTCCAATCCTCTCTTTGATAAGAACATTGCTACCTCAATCCAAATTCGCAAGGGAATATTTTGCTGCATTCACCACTGTCTTCTTTGCTTGGTTAGTTGGACCCTGCGAGGTGAAGGAATCGGACTTCAAAGGAAAAAGAGAAAAAAATGTGGTTCAAATCCACAAGTGCAGGTTCCTAGAGCAAACTAACTGTGCTGGAATGTGCATTAATCTATGCAAGTTTCCATGTCAGGATTTCATCAAGGATTCTTTGGGAATGCCAGTCAGCATGGTCCCCAATTTTGATGACATGAGCTGTGAGATGATATTTGGAAAGGATCCACCGGCTGCAGTCGATGATCCAGCGCTTAAGCAACCATGTTACAAACTATGCAAAACAAAAGAAAAACATACGACAAACTGCTTCATTTAG

Protein sequence

MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQPCYKLCKTKEKHTTNCFI
Homology
BLAST of CmoCh19G000130 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 6.3e-84
Identity = 145/232 (62.50%), Postives = 183/232 (78.88%), Query Frame = 0

Query: 24  KTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRS 83
           K  +++ K    V VL R  +   +EET +   VY DNWF+K+AI+H+S++VQA +G  +
Sbjct: 20  KKLRLKPKHTFVVGVLARPTDD-ISEETLRKTNVYKDNWFDKLAINHLSKSVQAATGISN 79

Query: 84  NKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAA 143
           NKSG++SLVE AT+AS+ +N  +Q+ +I+ AL RAFP PILS+IR ++P SK AREYFA 
Sbjct: 80  NKSGFDSLVEAATVASQKFNTTQQQGIILDALDRAFPKPILSVIRRVMPPSKLAREYFAV 139

Query: 144 FTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKD 203
           FTT+FFAWL+GP EV+ES+  G+REKN+V I KCRFLE+TNC GMCINLCK P Q FIKD
Sbjct: 140 FTTIFFAWLLGPSEVRESEINGRREKNIVHIKKCRFLEETNCVGMCINLCKMPSQLFIKD 199

Query: 204 SLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQPCYKLCKTKEKHTTNC 256
           S GMPV+MVPNFDDMSCEMIFG++PPA+ DDPALKQPCYKLCK K+ H T C
Sbjct: 200 SFGMPVNMVPNFDDMSCEMIFGQEPPASTDDPALKQPCYKLCKAKKNHATQC 250

BLAST of CmoCh19G000130 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.5e-69
Identity = 132/249 (53.01%), Postives = 170/249 (68.27%), Query Frame = 0

Query: 28  MRRKSIACVAVLTRAAESGATEETRK------------------PNTVYTDNWFEKIAIH 87
           MRR      AV+ R  E+ A+   +K                  P TVY D+WF+K+AI 
Sbjct: 32  MRRCCSTVRAVMARPQEAPASAPAKKTETAAMMSTVQTETAAAPPATVYRDSWFDKLAIG 91

Query: 88  HVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFPAPILSLIRT 147
           ++S+ +Q  SG ++ K GYESL++ A   SR ++  KQ E++ QAL RA P+ IL++I+ 
Sbjct: 92  YLSRNLQEASGLKNEKDGYESLIDAALAISRIFSLDKQSEIVTQALERALPSYILTMIKV 151

Query: 148 LLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFLEQTNCAGMC 207
           ++P S+F+REYFAAFTT+FF WLVGPCEV ES+ +G++EKNVV I KCRFLE TNC GMC
Sbjct: 152 MMPPSRFSREYFAAFTTIFFPWLVGPCEVMESEVEGRKEKNVVYIPKCRFLESTNCVGMC 211

Query: 208 INLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQPCYKL-CKTK 258
            NLCK PCQ FI+DSLGM V M PNF+DMSCEMIFG+ PP   DDPALKQPC++  C  K
Sbjct: 212 TNLCKIPCQKFIQDSLGMKVYMSPNFEDMSCEMIFGQQPPE--DDPALKQPCFRTKCVAK 271

BLAST of CmoCh19G000130 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.0e-57
Identity = 104/215 (48.37%), Postives = 151/215 (70.23%), Query Frame = 0

Query: 39  LTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRSN--KSGYESLVEVAT 98
           L  A E+   E +   N    D++F KIAI+++S+ +Q  +G  S+   + Y+ LV+ AT
Sbjct: 46  LKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTAT 105

Query: 99  MASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPC 158
             SRN++  +Q E ++ +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP 
Sbjct: 106 RVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPS 165

Query: 159 EVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFD 218
           EV+E++  G++EK+VV I KCRFLEQ+NC GMC ++CK P Q FIK+SLGMP+ M P+F+
Sbjct: 166 EVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFN 225

Query: 219 DMSCEMIFGKDPPAAVDDPALKQPCYKLCKTKEKH 252
           D+SC+M+FG++PP   DDPA+KQPC++ CK+ + +
Sbjct: 226 DLSCKMMFGREPPEIEDDPAMKQPCFEFCKSNKSY 260

BLAST of CmoCh19G000130 vs. ExPASy TrEMBL
Match: A0A6J1HHC5 (beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111464069 PE=4 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.1e-145
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0

Query: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCKTKEKHTTNCFI 258
           CYKLCKTKEKHTTNCFI
Sbjct: 241 CYKLCKTKEKHTTNCFI 257

BLAST of CmoCh19G000130 vs. ExPASy TrEMBL
Match: A0A6J1HUP9 (beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111466998 PE=4 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 2.8e-143
Identity = 254/257 (98.83%), Postives = 255/257 (99.22%), Query Frame = 0

Query: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEVRVGE RRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEERRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRS KSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSKKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGK+EKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKKEKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLCKTKEKHTTNCFI 258
           CYKLCKTKEKHTTNCFI
Sbjct: 241 CYKLCKTKEKHTTNCFI 257

BLAST of CmoCh19G000130 vs. ExPASy TrEMBL
Match: A0A6J1HLI8 (beta-carotene isomerase D27, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464069 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 3.9e-137
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0

Query: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLC 246
           CYKLC
Sbjct: 241 CYKLC 245

BLAST of CmoCh19G000130 vs. ExPASy TrEMBL
Match: A0A6J1HJA1 (beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464069 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 3.9e-137
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0

Query: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLC 246
           CYKLC
Sbjct: 241 CYKLC 245

BLAST of CmoCh19G000130 vs. ExPASy TrEMBL
Match: A0A6J1HWX8 (beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466998 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.1e-135
Identity = 242/245 (98.78%), Postives = 243/245 (99.18%), Query Frame = 0

Query: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60
           MEVRVGE RRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD
Sbjct: 1   MEVRVGEERRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120
           NWFEKIAIHHVSQAVQATSGWRS KSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSKKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRFL 180
           APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGK+EKNVVQIHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKKEKNVVQIHKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240
           EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYKLC 246
           CYKLC
Sbjct: 241 CYKLC 245

BLAST of CmoCh19G000130 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 222.6 bits (566), Expect = 3.6e-58
Identity = 104/215 (48.37%), Postives = 151/215 (70.23%), Query Frame = 0

Query: 39  LTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRSN--KSGYESLVEVAT 98
           L  A E+   E +   N    D++F KIAI+++S+ +Q  +G  S+   + Y+ LV+ AT
Sbjct: 46  LKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTAT 105

Query: 99  MASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPC 158
             SRN++  +Q E ++ +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP 
Sbjct: 106 RVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPS 165

Query: 159 EVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFD 218
           EV+E++  G++EK+VV I KCRFLEQ+NC GMC ++CK P Q FIK+SLGMP+ M P+F+
Sbjct: 166 EVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFN 225

Query: 219 DMSCEMIFGKDPPAAVDDPALKQPCYKLCKTKEKH 252
           D+SC+M+FG++PP   DDPA+KQPC++ CK+ + +
Sbjct: 226 DLSCKMMFGREPPEIEDDPAMKQPCFEFCKSNKSY 260

BLAST of CmoCh19G000130 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 154.1 bits (388), Expect = 1.6e-37
Identity = 69/154 (44.81%), Postives = 101/154 (65.58%), Query Frame = 0

Query: 88  YESLVEVATMASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTV 147
           YES VEV+    +  ++++Q+E + + L    P       R L P +K+A E+ AA T  
Sbjct: 77  YESFVEVSKRVMQGRSRVQQQEAVREVLLSMLPPGAPEQFRKLFPPTKWAAEFNAALTVP 136

Query: 148 FFAWLVGPCEVKESDFKGKREKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGM 207
           FF WLVGP +V E +  G ++++ V+I KCR+LE + C GMC+N+CK P QDF  +  G+
Sbjct: 137 FFHWLVGPSQVIEVEVNGVKQRSGVRIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGL 196

Query: 208 PVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQPC 242
           P++M PN++DMSCEMI+G+ PPA  +D A KQPC
Sbjct: 197 PLTMNPNYEDMSCEMIYGQAPPAFEEDVATKQPC 230

BLAST of CmoCh19G000130 vs. TAIR 10
Match: AT1G03055.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 118.6 bits (296), Expect = 7.3e-27
Identity = 62/142 (43.66%), Postives = 91/142 (64.08%), Query Frame = 0

Query: 39  LTRAAESGATEETRKPNTVYTDNWFEKIAIHHVSQAVQATSGWRSN--KSGYESLVEVAT 98
           L  A E+   E +   N    D++F KIAI+++S+ +Q  +G  S+   + Y+ LV+ AT
Sbjct: 46  LKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTAT 105

Query: 99  MASRNYNQIKQREVIIQALGRAFPAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPC 158
             SRN++  +Q E ++ +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP 
Sbjct: 106 RVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPS 165

Query: 159 EVKESDFKGKREKNVVQIHKCR 179
           EV+E++  G++EK+VV I KCR
Sbjct: 166 EVRETEVNGRKEKSVVYIEKCR 187

BLAST of CmoCh19G000130 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 109.0 bits (271), Expect = 5.7e-24
Identity = 78/245 (31.84%), Postives = 123/245 (50.20%), Query Frame = 0

Query: 10  RVILGSGTNVRRSN--KTKKMRRKSIACVAVLTRAAES--GATEETRKPNTVYTDNWFEK 69
           +V+L   T +  SN   TK +  + + C    +   +S  GA +   KP  +  D++F +
Sbjct: 6   QVLLPPPTTIVFSNSFSTKLILTRRLRCRISNSSEVKSDEGAPKLEYKPGPL--DDFFMQ 65

Query: 70  IAIHHVSQAVQATSGWRSNKSGYESLVE-VATMASRNYNQIKQREVIIQALGRAFPAPIL 129
              + + + V    G  S K GY  L+E V  +  +   + +  +  ++ L   FP  IL
Sbjct: 66  SFRNKLVEEV----GSDSEKPGYVGLIELVKLLLLKGRTRSETSDAAVRILKSLFPPLIL 125

Query: 130 SLIRTL---LPQSKFAREYFAAFTTVFFAWLVGPCEVKESDF-KGKREKNVVQIHKCRFL 189
            L + L   + Q K A    A  T +   WL+GP +V   D   G+   + V + KC++L
Sbjct: 126 ELYKLLIAPIAQGKLAALMVARVTVLTCQWLMGPSKVNIIDLPNGESWDSGVFVEKCQYL 185

Query: 190 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 246
           E++ C G+CIN CK P Q F KD +G+P+ M PNF D SC+  FG  PP   DD  + +P
Sbjct: 186 EESKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKDYSCQFKFGVAPPE--DDGNVNEP 242

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
R4HZ966.3e-8462.50Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27... [more]
C7AU211.5e-6953.01Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39... [more]
Q7XA785.0e-5748.37Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D2... [more]
Match NameE-valueIdentityDescription
A0A6J1HHC55.1e-145100.00beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita moschata... [more]
A0A6J1HUP92.8e-14398.83beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita maxima O... [more]
A0A6J1HLI83.9e-137100.00beta-carotene isomerase D27, chloroplastic-like isoform X1 OS=Cucurbita moschata... [more]
A0A6J1HJA13.9e-137100.00beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita moschata... [more]
A0A6J1HWX82.1e-13598.78beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita maxima O... [more]
Match NameE-valueIdentityDescription
AT1G03055.13.6e-5848.37unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G64680.11.6e-3744.81unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G03055.27.3e-2743.66unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT4G01995.15.7e-2431.84unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025114Beta-carotene isomerase D27-like, C-terminalPFAMPF13225DUF4033coord: 151..230
e-value: 3.8E-33
score: 113.3
NoneNo IPR availablePANTHERPTHR33591:SF1BETA-CAROTENE ISOMERASE D27, CHLOROPLASTICcoord: 13..253
IPR038938Beta-carotene isomerase D27-likePANTHERPTHR33591BETA-CAROTENE ISOMERASE D27coord: 13..253

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh19G000130.1CmoCh19G000130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010223 secondary shoot formation
biological_process GO:1901601 strigolactone biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0016859 cis-trans isomerase activity
molecular_function GO:0005506 iron ion binding