CmoCh18G013790 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh18G013790
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionHeavy metal-associated domain containing protein
LocationCmo_Chr18: 12854805 .. 12855318 (-)
RNA-Seq ExpressionCmoCh18G013790
SyntenyCmoCh18G013790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAATTTGCAAATAGTTCCGGCTCACACCCCATTTCATGTGGAGCCTCAGCTGGTGGAGATGATGGTTCCCCTTTATTCTCATGGTTGCGCCAACAAAATCCACAAGGCTCTCTCCCATCTCAAAGGTCTTATTTTGATTTTTAGTACAACACCCATCTATATATTCTCCAAAACCTCACACTAACAACATGCATGGTATGATTGCAGGGATATATTCAGTTAACGTAGATTACGAGGAGCAGAAAGTGACGGTGTGGGGAATATGCAACAAAAACCAAGTGTTGAGCACTGTGAGAAGCAAGAGAAAAGCAGCTCGTTTTTGGAACAATGATGATGATGATAACAATAATAATGATCCACAAGCAGAAGAAGAAGGTCTAAACTCAAGAAGGCCTTCTTTTGGTGTTAACAAGCTTCGTTCCCTCAGTTTCAAATTAGCCTGCAAGAAGGTTTTCACCACTACAAGATCATATTCCTTGCGTATCTTTGGGGCTCCTACTGTTTCCTAA

mRNA sequence

ATGGCTAATTTGCAAATAGTTCCGGCTCACACCCCATTTCATGTGGAGCCTCAGCTGGTGGAGATGATGGTTCCCCTTTATTCTCATGGTTGCGCCAACAAAATCCACAAGGCTCTCTCCCATCTCAAAGTACAACACCCATCTATATATTCTCCAAAACCTCACACTAACAACATGCATGGTATGATTGCAGGGATATATTCAGTTAACGTAGATTACGAGGAGCAGAAAGTGACGGTGTGGGGAATATGCAACAAAAACCAAGTGTTGAGCACTGTGAGAAGCAAGAGAAAAGCAGCTCGTTTTTGGAACAATGATGATGATGATAACAATAATAATGATCCACAAGCAGAAGAAGAAGGTCTAAACTCAAGAAGGCCTTCTTTTGGTGTTAACAAGCTTCGTTCCCTCAGTTTCAAATTAGCCTGCAAGAAGGTTTTCACCACTACAAGATCATATTCCTTGCGTATCTTTGGGGCTCCTACTGTTTCCTAA

Coding sequence (CDS)

ATGGCTAATTTGCAAATAGTTCCGGCTCACACCCCATTTCATGTGGAGCCTCAGCTGGTGGAGATGATGGTTCCCCTTTATTCTCATGGTTGCGCCAACAAAATCCACAAGGCTCTCTCCCATCTCAAAGTACAACACCCATCTATATATTCTCCAAAACCTCACACTAACAACATGCATGGTATGATTGCAGGGATATATTCAGTTAACGTAGATTACGAGGAGCAGAAAGTGACGGTGTGGGGAATATGCAACAAAAACCAAGTGTTGAGCACTGTGAGAAGCAAGAGAAAAGCAGCTCGTTTTTGGAACAATGATGATGATGATAACAATAATAATGATCCACAAGCAGAAGAAGAAGGTCTAAACTCAAGAAGGCCTTCTTTTGGTGTTAACAAGCTTCGTTCCCTCAGTTTCAAATTAGCCTGCAAGAAGGTTTTCACCACTACAAGATCATATTCCTTGCGTATCTTTGGGGCTCCTACTGTTTCCTAA

Protein sequence

MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLKVQHPSIYSPKPHTNNMHGMIAGIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNNDDDDNNNNDPQAEEEGLNSRRPSFGVNKLRSLSFKLACKKVFTTTRSYSLRIFGAPTVS
Homology
BLAST of CmoCh18G013790 vs. ExPASy TrEMBL
Match: A0A6J1G1N8 (protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Cucurbita moschata OX=3662 GN=LOC111449858 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 4.0e-71
Identity = 143/164 (87.20%), Postives = 143/164 (87.20%), Query Frame = 0

Query: 1   MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLKVQHPSIYSPKPHTNNMH 60
           MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLK                 
Sbjct: 1   MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLK----------------- 60

Query: 61  GMIAGIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNNDDDDNNNNDPQAEEE 120
               GIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNNDDDDNNNNDPQAEEE
Sbjct: 61  ----GIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNNDDDDNNNNDPQAEEE 120

Query: 121 GLNSRRPSFGVNKLRSLSFKLACKKVFTTTRSYSLRIFGAPTVS 165
           GLNSRRPSFGVNKLRSLSFKLACKKVFTTTRSYSLRIFGAPTVS
Sbjct: 121 GLNSRRPSFGVNKLRSLSFKLACKKVFTTTRSYSLRIFGAPTVS 143

BLAST of CmoCh18G013790 vs. ExPASy TrEMBL
Match: A0A6J1HXU2 (heavy metal-associated isoprenylated plant protein 28 OS=Cucurbita maxima OX=3661 GN=LOC111467638 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.5e-68
Identity = 141/168 (83.93%), Postives = 142/168 (84.52%), Query Frame = 0

Query: 1   MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLKVQHPSIYSPKPHTNNMH 60
           MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLK                 
Sbjct: 1   MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLK----------------- 60

Query: 61  GMIAGIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNNDDDD----NNNNDPQ 120
               GIYSVNVDY+EQKVTVWGICNKNQVLSTVRSKRKAARFWN DDDD    NNNNDPQ
Sbjct: 61  ----GIYSVNVDYDEQKVTVWGICNKNQVLSTVRSKRKAARFWNKDDDDNNNNNNNNDPQ 120

Query: 121 AEEEGLNSRRPSFGVNKLRSLSFKLACKKVFTTTRSYSLRIFGAPTVS 165
           AEEEGLNSRRPSFGVNKLRSLSFKLACKKVFTTTRSYSLRIFGAPTVS
Sbjct: 121 AEEEGLNSRRPSFGVNKLRSLSFKLACKKVFTTTRSYSLRIFGAPTVS 147

BLAST of CmoCh18G013790 vs. ExPASy TrEMBL
Match: A0A6J1CHX5 (uncharacterized protein LOC111011661 OS=Momordica charantia OX=3673 GN=LOC111011661 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 6.7e-34
Identity = 97/162 (59.88%), Postives = 107/162 (66.05%), Query Frame = 0

Query: 1   MANLQIVPAHT--PFHVEPQLVEMMVPLYSHGCANKIHKALSHLKVQHPSIYSPKPHTNN 60
           MA L IVPA T   ++VEPQLVEMMVPLYSHGCANK+HKALSHLK               
Sbjct: 3   MAKLHIVPADTRYSYNVEPQLVEMMVPLYSHGCANKVHKALSHLK--------------- 62

Query: 61  MHGMIAGIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNND-DDDNNNNDPQA 120
                 GIYSVNVD  +QKVTVWGICNK QVLSTVRSKRKAARFWN++ + + +N+ P  
Sbjct: 63  ------GIYSVNVDCNQQKVTVWGICNKYQVLSTVRSKRKAARFWNHETEQEADNSGPVV 122

Query: 121 EEEGLN-SRRPSFGVNKLRSLSFKLACKKV--FTTTRSYSLR 157
                  S RP F V   RSLS KLACKKV   TT RSYS R
Sbjct: 123 PPPAPPLSFRPRFAV---RSLSLKLACKKVLNLTTPRSYSFR 140

BLAST of CmoCh18G013790 vs. ExPASy TrEMBL
Match: M0ZIL1 (HMA domain-containing protein OS=Solanum tuberosum OX=4113 GN=102593133 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 1.2e-30
Identity = 83/154 (53.90%), Postives = 99/154 (64.29%), Query Frame = 0

Query: 1   MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLKVQHPSIYSPKPHTNNMH 60
           MAN+QIVPA    +VEPQ VEMMVPLYS+GC  KI+ ALSH+K                 
Sbjct: 1   MANMQIVPAGRNVNVEPQYVEMMVPLYSYGCERKINNALSHIK----------------- 60

Query: 61  GMIAGIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNNDDDDNNNNDPQAEEE 120
               GIYSVNVD+E+QKVTVWGICNK  VL+TVRSKRKAARFWN +D+    ++  +   
Sbjct: 61  ----GIYSVNVDFEQQKVTVWGICNKYDVLATVRSKRKAARFWNTEDNMEEYSETDSSAT 120

Query: 121 GLNSRRPSFGVNKLRSLSFKLACKKVFTTTRSYS 155
                RP   + + RSLS+KLA KK F  TRSYS
Sbjct: 121 CRYRIRPPLALIRARSLSWKLALKKAF--TRSYS 131

BLAST of CmoCh18G013790 vs. ExPASy TrEMBL
Match: A0A3Q7FME6 (HMA domain-containing protein OS=Solanum lycopersicum OX=4081 GN=101252962 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 2.0e-30
Identity = 83/160 (51.88%), Postives = 101/160 (63.12%), Query Frame = 0

Query: 1   MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLKVQHPSIYSPKPHTNNMH 60
           MAN+QIVPA    +VEPQ VEMMVPLYS+GC  KI+ ALSH+K                 
Sbjct: 1   MANMQIVPAGRNVNVEPQYVEMMVPLYSYGCERKINNALSHIK----------------- 60

Query: 61  GMIAGIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNNDDD------DNNNND 120
               GIYSVNVD+E+QKVT+WGICNK  VL+ VRSKRKAARFWN +D+      D++++ 
Sbjct: 61  ----GIYSVNVDFEQQKVTIWGICNKYDVLARVRSKRKAARFWNEEDNMEESERDSSSSS 120

Query: 121 PQAEEEGLNSRRPSFGVNKLRSLSFKLACKKVFTTTRSYS 155
           P A    +  R     + + RSLS KLA KK F  TRSYS
Sbjct: 121 PAASRYRIRPRAQPLALIRARSLSLKLALKKAF--TRSYS 137

BLAST of CmoCh18G013790 vs. TAIR 10
Match: AT4G27590.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 2.8e-24
Identity = 60/112 (53.57%), Postives = 71/112 (63.39%), Query Frame = 0

Query: 1   MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLKVQHPSIYSPKPHTNNMH 60
           M +LQIVP +    VE Q VEMMVPLYS+GC  K+ +ALSHLK                 
Sbjct: 1   MGDLQIVPVYN--KVEAQYVEMMVPLYSYGCEKKVKRALSHLK----------------- 60

Query: 61  GMIAGIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNNDDDDNNN 113
               GIYSV VDY  QKVTVWGICNK  VL+ V+ KRK ARFWN ++++NNN
Sbjct: 61  ----GIYSVKVDYYNQKVTVWGICNKLDVLAMVKKKRKEARFWNVEEEENNN 89

BLAST of CmoCh18G013790 vs. TAIR 10
Match: AT4G27590.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 2.8e-24
Identity = 60/112 (53.57%), Postives = 71/112 (63.39%), Query Frame = 0

Query: 1   MANLQIVPAHTPFHVEPQLVEMMVPLYSHGCANKIHKALSHLKVQHPSIYSPKPHTNNMH 60
           M +LQIVP +    VE Q VEMMVPLYS+GC  K+ +ALSHLK                 
Sbjct: 1   MGDLQIVPVYN--KVEAQYVEMMVPLYSYGCEKKVKRALSHLK----------------- 60

Query: 61  GMIAGIYSVNVDYEEQKVTVWGICNKNQVLSTVRSKRKAARFWNNDDDDNNN 113
               GIYSV VDY  QKVTVWGICNK  VL+ V+ KRK ARFWN ++++NNN
Sbjct: 61  ----GIYSVKVDYYNQKVTVWGICNKLDVLAMVKKKRKEARFWNVEEEENNN 89

BLAST of CmoCh18G013790 vs. TAIR 10
Match: AT3G56891.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 2.5e-04
Identity = 23/85 (27.06%), Postives = 40/85 (47.06%), Query Frame = 0

Query: 19  LVEMMVPLYSHGCANKIHKALSHLKVQHPSIYSPKPHTNNMHGMIAGIYSVNVDYEEQKV 78
           +VE++V +   GC  K+ +A+S L                      G+ +V +D + QKV
Sbjct: 17  IVELLVDMDCKGCEKKVRRAISKLD---------------------GVDTVEIDVDRQKV 76

Query: 79  TVWGICNKNQVLSTVRSKRKAARFW 104
           TV G  ++ +VL  V+   + A +W
Sbjct: 77  TVTGYVDREEVLKMVKRTGRTAEYW 80

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1G1N84.0e-7187.20protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1HXU22.5e-6883.93heavy metal-associated isoprenylated plant protein 28 OS=Cucurbita maxima OX=366... [more]
A0A6J1CHX56.7e-3459.88uncharacterized protein LOC111011661 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
M0ZIL11.2e-3053.90HMA domain-containing protein OS=Solanum tuberosum OX=4113 GN=102593133 PE=4 SV=... [more]
A0A3Q7FME62.0e-3051.88HMA domain-containing protein OS=Solanum lycopersicum OX=4081 GN=101252962 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT4G27590.12.8e-2453.57Heavy metal transport/detoxification superfamily protein [more]
AT4G27590.22.8e-2453.57Heavy metal transport/detoxification superfamily protein [more]
AT3G56891.12.5e-0427.06Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 16..102
e-value: 3.4E-9
score: 38.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..126
NoneNo IPR availablePANTHERPTHR22814:SF305HEAVY METAL TRANSPORT/DETOXIFICATION SUPERFAMILY PROTEINcoord: 1..43
coord: 63..126
NoneNo IPR availablePANTHERPTHR22814COPPER TRANSPORT PROTEIN ATOX1-RELATEDcoord: 1..43
coord: 63..126
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 21..82
e-value: 1.3612E-5
score: 39.1261
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 20..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh18G013790.1CmoCh18G013790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding