CmoCh18G013460 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh18G013460
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pectinesterase
Location	Cmo_Chr18: 12739181 .. 12741541 (+)
RNA-Seq Expression	CmoCh18G013460
Synteny	CmoCh18G013460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTCCTCTCCTCTCCTCTCCTCTCCTCTCCGAAAATGGGGTGCCGCCGCCTCAATGAAACCCACCCTGCTGCCAACCCAAGGAGAGGACCACTTTCCGTTCTCTCTCTTCTGTCCCTCATTCTCATCCTCCCCGCCGTCTCCCCCACTTCACTCCTCGGACTCCGAACCTCTTCCGACCCGAAACTCCCTCGAAAACCGACCCGGGCAATTTCCAAGGCCTGTGCCCGAAGTCGGCTCCCGGCTCTGTGCGTGGACTCGCTCCTGGATTTCCCTGGATCTCTGAGCGCCAACGAACGAGATCTCGTTCACATTTCCTTCAATTTGACTCTCCACCATCTCAATGAAGCCCTCTACTCATCCTCCCACCTTTCCTCTCTTCATATGGATCTTCCCCTTCGCTCCGCCTACGACGCCTGTCTTGAACTCCTCGATGATTCCGTCCATGCCCTAACCCGCTCTTTTCACTCCCTTGCGCCGCCTTCCTCCGACAGCCGCCCAACTCCCCTCGCCTCCTCCTCCGATGACGTCATCACCTGGCTCAGCGCCGCCCTCACCAATCAAGACACCTGTACGGACGGATTCTCCCAACTCAGCGGCAATGTTAAAGATCAAATGACTGACAAACTCCACAATTTGTCTGAGCTTGTGAGCAATTGCCTGGCGTTGTTTTCCGGTAGCGTAAGGAACGACTTTTCCGGAGTGCCGATTCAGAACAAGCGACGGTTGATGAACGCCGCCGAGAAGGAGGATGATTCTGGTAACTTCCCCGGGTGGATGAGCCGGAGAGAGAGGCGGTTGCTGACGTTGCCGGTTGGGGCGTTGCAGGCCGATATTGTGGTTTCGAAGGACGGGAATGGAACGGTAAAGACGATCGCCGAAGCGATAAAAAAGGCACCGGAATTCAGTACCAGAAAGACGATCATCTACGTGATGGCAGGAAGGTAAATATAAATGTACACTTGAATTAATATTTTTGAATTACGATTTGGCATGATGAATTTACGGAAATACCCTTTTGTCCTTTCTACCTACAGGTACGAGGAGAAAAACCTGAAGGTAGGGAGGAAGAAAACGAATCTGATGTTTGTTGGGGATGGGAAGGGTAAAACTGTGATTTCGGGCGGTAAGAGTATCTTTGACAACGTGACAACCTTTCACACGGCGTCGTTTGGTAAAATAAATTATTTTCAACCATTTCTCTAATTAATTTTATTTATTTATTTATTTATTTACTTCAATTACCCTAACCCCTAATCTAATATGATTTCTCAATTGCATGGGAATTTCATTAATTTATTCCTCTCAAAGATTTATAGTATAATATTTTACCCATCCATCTGAGTTTCTTTATTATTATTTTTTTTTTTAAATTTTTTGGGGCCACAGCCGTCAGCGGCGCCGGTATCATTTTGAGGGACATGACATTTGTGAATTGGGCCGGACCGGCTCGGCACCAGGCGGTGGCCCTCCGAGTCAGCGCCGATCACGCCGTCGTGTACCGGTGCAACGTCATCGGGTATCAGGACACTCTCTACGTGCATTCGAACCGGCAATTCTTTCGGGAGTGCGACATTTACGGGACGGTGGATTTCATCTTCGGCAATGCGGCGGTGGTGTTTCAAAACTGCAGCATTTACGCCCGAAAGGCGATGGAGCTTCAAAAGAACACAATCACGGCTCAGAACCGGAAGGATCCGAATCAGAACACTGGAATTTCCATCCACGCTTGCCGGATTTTGGCCACCCCCGATCTGGCGTCGTCCTCAACTGCCACCAACCCAACGTATCTGGGTCGTCCCTGGAAACTATACTCTAGAGTTGTGTACATGCAGTCATACATGGGGGGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAACGCCACGTTTGCACTTGACACCTTGTATTACGGTGAGTATATGAACTATGGGCCGGGTGGGGCAGTGGGCCAGAGGGTGACCTGGCCTGGATATCGGGTCATAACGTCGAGGGTGGAGGCAAGTCAGTTCACGGTGGCGCAGTTCATTTACGGCTCGTCGTGGCTACCGTCCACCGGAGTGGCTTTCCTGGGAGGGCTAAATCTGTAATTCGGCCACCTCTATTGGACTAAGGTGCGTAATAAAGAAGGAAGAAAAACAAGAAACCGGAGAAAAAGGTACGTAAAAAGTGTCTGCATAAAATACTATATAATTAACCGTGATTTCTTTTTTTGTTTTTTTAAAATAATTTTTGGTACGGTGAATGTATGAATATTAATTTATGTTTCATTCTTTTGTTTTTTTTTTTTTTTTCCTTCCCCTCTTATTTGTATTGTAACAGAATAATATTTGATTATATATATATATATCATTAATAAGGC

mRNA sequence

CTCTCCTCTCCTCTCCTCTCCTCTCCTCTCCGAAAATGGGGTGCCGCCGCCTCAATGAAACCCACCCTGCTGCCAACCCAAGGAGAGGACCACTTTCCGTTCTCTCTCTTCTGTCCCTCATTCTCATCCTCCCCGCCGTCTCCCCCACTTCACTCCTCGGACTCCGAACCTCTTCCGACCCGAAACTCCCTCGAAAACCGACCCGGGCAATTTCCAAGGCCTGTGCCCGAAGTCGGCTCCCGGCTCTGTGCGTGGACTCGCTCCTGGATTTCCCTGGATCTCTGAGCGCCAACGAACGAGATCTCGTTCACATTTCCTTCAATTTGACTCTCCACCATCTCAATGAAGCCCTCTACTCATCCTCCCACCTTTCCTCTCTTCATATGGATCTTCCCCTTCGCTCCGCCTACGACGCCTGTCTTGAACTCCTCGATGATTCCGTCCATGCCCTAACCCGCTCTTTTCACTCCCTTGCGCCGCCTTCCTCCGACAGCCGCCCAACTCCCCTCGCCTCCTCCTCCGATGACGTCATCACCTGGCTCAGCGCCGCCCTCACCAATCAAGACACCTGTACGGACGGATTCTCCCAACTCAGCGGCAATGTTAAAGATCAAATGACTGACAAACTCCACAATTTGTCTGAGCTTGTGAGCAATTGCCTGGCGTTGTTTTCCGGTAGCGTAAGGAACGACTTTTCCGGAGTGCCGATTCAGAACAAGCGACGGTTGATGAACGCCGCCGAGAAGGAGGATGATTCTGGTAACTTCCCCGGGTGGATGAGCCGGAGAGAGAGGCGGTTGCTGACGTTGCCGGTTGGGGCGTTGCAGGCCGATATTGTGGTTTCGAAGGACGGGAATGGAACGGTAAAGACGATCGCCGAAGCGATAAAAAAGGCACCGGAATTCAGTACCAGAAAGACGATCATCTACGTGATGGCAGGAAGGTACGAGGAGAAAAACCTGAAGGTAGGGAGGAAGAAAACGAATCTGATGTTTGTTGGGGATGGGAAGGGTAAAACTGTGATTTCGGGCGGTAAGAGTATCTTTGACAACGTGACAACCTTTCACACGGCGTCGTTTGCCGTCAGCGGCGCCGGTATCATTTTGAGGGACATGACATTTGTGAATTGGGCCGGACCGGCTCGGCACCAGGCGGTGGCCCTCCGAGTCAGCGCCGATCACGCCGTCGTGTACCGGTGCAACGTCATCGGGTATCAGGACACTCTCTACGTGCATTCGAACCGGCAATTCTTTCGGGAGTGCGACATTTACGGGACGGTGGATTTCATCTTCGGCAATGCGGCGGTGGTGTTTCAAAACTGCAGCATTTACGCCCGAAAGGCGATGGAGCTTCAAAAGAACACAATCACGGCTCAGAACCGGAAGGATCCGAATCAGAACACTGGAATTTCCATCCACGCTTGCCGGATTTTGGCCACCCCCGATCTGGCGTCGTCCTCAACTGCCACCAACCCAACGTATCTGGGTCGTCCCTGGAAACTATACTCTAGAGTTGTGTACATGCAGTCATACATGGGGGGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAACGCCACGTTTGCACTTGACACCTTGTATTACGGTGAGTATATGAACTATGGGCCGGGTGGGGCAGTGGGCCAGAGGGTGACCTGGCCTGGATATCGGGTCATAACGTCGAGGGTGGAGGCAAGTCAGTTCACGGTGGCGCAGTTCATTTACGGCTCGTCGTGGCTACCGTCCACCGGAGTGGCTTTCCTGGGAGGGCTAAATCTGTAATTCGGCCACCTCTATTGGACTAAGGTGCGTAATAAAGAAGGAAGAAAAACAAGAAACCGGAGAAAAAGGTACGTAAAAAGTGTCTGCATAAAATACTATATAATTAACCGTGATTTCTTTTTTTGTTTTTTTAAAATAATTTTTGGTACGGTGAATGTATGAATATTAATTTATGTTTCATTCTTTTGTTTTTTTTTTTTTTTTCCTTCCCCTCTTATTTGTATTGTAACAGAATAATATTTGATTATATATATATATATCATTAATAAGGC

Coding sequence (CDS)

ATGGGGTGCCGCCGCCTCAATGAAACCCACCCTGCTGCCAACCCAAGGAGAGGACCACTTTCCGTTCTCTCTCTTCTGTCCCTCATTCTCATCCTCCCCGCCGTCTCCCCCACTTCACTCCTCGGACTCCGAACCTCTTCCGACCCGAAACTCCCTCGAAAACCGACCCGGGCAATTTCCAAGGCCTGTGCCCGAAGTCGGCTCCCGGCTCTGTGCGTGGACTCGCTCCTGGATTTCCCTGGATCTCTGAGCGCCAACGAACGAGATCTCGTTCACATTTCCTTCAATTTGACTCTCCACCATCTCAATGAAGCCCTCTACTCATCCTCCCACCTTTCCTCTCTTCATATGGATCTTCCCCTTCGCTCCGCCTACGACGCCTGTCTTGAACTCCTCGATGATTCCGTCCATGCCCTAACCCGCTCTTTTCACTCCCTTGCGCCGCCTTCCTCCGACAGCCGCCCAACTCCCCTCGCCTCCTCCTCCGATGACGTCATCACCTGGCTCAGCGCCGCCCTCACCAATCAAGACACCTGTACGGACGGATTCTCCCAACTCAGCGGCAATGTTAAAGATCAAATGACTGACAAACTCCACAATTTGTCTGAGCTTGTGAGCAATTGCCTGGCGTTGTTTTCCGGTAGCGTAAGGAACGACTTTTCCGGAGTGCCGATTCAGAACAAGCGACGGTTGATGAACGCCGCCGAGAAGGAGGATGATTCTGGTAACTTCCCCGGGTGGATGAGCCGGAGAGAGAGGCGGTTGCTGACGTTGCCGGTTGGGGCGTTGCAGGCCGATATTGTGGTTTCGAAGGACGGGAATGGAACGGTAAAGACGATCGCCGAAGCGATAAAAAAGGCACCGGAATTCAGTACCAGAAAGACGATCATCTACGTGATGGCAGGAAGGTACGAGGAGAAAAACCTGAAGGTAGGGAGGAAGAAAACGAATCTGATGTTTGTTGGGGATGGGAAGGGTAAAACTGTGATTTCGGGCGGTAAGAGTATCTTTGACAACGTGACAACCTTTCACACGGCGTCGTTTGCCGTCAGCGGCGCCGGTATCATTTTGAGGGACATGACATTTGTGAATTGGGCCGGACCGGCTCGGCACCAGGCGGTGGCCCTCCGAGTCAGCGCCGATCACGCCGTCGTGTACCGGTGCAACGTCATCGGGTATCAGGACACTCTCTACGTGCATTCGAACCGGCAATTCTTTCGGGAGTGCGACATTTACGGGACGGTGGATTTCATCTTCGGCAATGCGGCGGTGGTGTTTCAAAACTGCAGCATTTACGCCCGAAAGGCGATGGAGCTTCAAAAGAACACAATCACGGCTCAGAACCGGAAGGATCCGAATCAGAACACTGGAATTTCCATCCACGCTTGCCGGATTTTGGCCACCCCCGATCTGGCGTCGTCCTCAACTGCCACCAACCCAACGTATCTGGGTCGTCCCTGGAAACTATACTCTAGAGTTGTGTACATGCAGTCATACATGGGGGGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAACGCCACGTTTGCACTTGACACCTTGTATTACGGTGAGTATATGAACTATGGGCCGGGTGGGGCAGTGGGCCAGAGGGTGACCTGGCCTGGATATCGGGTCATAACGTCGAGGGTGGAGGCAAGTCAGTTCACGGTGGCGCAGTTCATTTACGGCTCGTCGTGGCTACCGTCCACCGGAGTGGCTTTCCTGGGAGGGCTAAATCTGTAA

Protein sequence

MGCRRLNETHPAANPRRGPLSVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL

Homology

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BLAST of CmoCh18G013460 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 1.4e-216
Identity = 386/564 (68.44%), Postives = 451/564 (79.96%), Query Frame = 0


Query: 21  SVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAISKACARSRLPALCVDSLLDFP 80
           S++  +SLIL     +          S P L RKP++AISKAC  +R P LCVDSL+DFP
Sbjct: 49  SIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFP 108

Query: 81  GSLSA-NERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHAL 140
           GSL+A + +DL+H++ N+TLHH + ALYSS+ LS + M    RSAYD+C+ELLDDSV AL
Sbjct: 109 GSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDAL 168

Query: 141 TRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQL-SGNVKDQMTDKL 200
           +R+  S+   SS ++P        DV TWLSAALTN DTCT+GF  +  G VKD MT  L
Sbjct: 169 SRALSSVV--SSSAKP-------QDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAAL 228

Query: 201 HNLSELVSNCLALFSGS-VRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLT 260
            NLSELVSNCLA+FS S   +DF+GVPIQN RRL+   E+E+    FP WM  +ER +L 
Sbjct: 229 QNLSELVSNCLAIFSASHDGDDFAGVPIQN-RRLLGVEEREE---KFPRWMRPKEREILE 288

Query: 261 LPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTN 320
           +PV  +QADI+VSKDGNGT KTI+EAIKKAP+ STR+ IIYV AGRYEE NLKVGRKK N
Sbjct: 289 MPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKIN 348

Query: 321 LMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALR 380
           LMFVGDGKGKTVISGGKSIFDN+TTFHTASFA +GAG I RD+TF NWAGPA+HQAVALR
Sbjct: 349 LMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALR 408

Query: 381 VSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAM 440
           + ADHAV+YRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVV QNCSIYARK M
Sbjct: 409 IGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPM 468

Query: 441 ELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQ 500
           + QKNTITAQNRKDPNQNTGISIHA R+LA  DL +++ +T  TYLGRPWKL+SR VYM 
Sbjct: 469 DFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQ-TYLGRPWKLFSRTVYMM 528

Query: 501 SYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFT 560
           SY+GGHVH RGWLEWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI S  EA++FT
Sbjct: 529 SYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFT 588

Query: 561 VAQFIYGSSWLPSTGVAFLGGLNL 582
           VA+FIYGSSWLPSTGV+FL GL++
Sbjct: 589 VAEFIYGSSWLPSTGVSFLAGLSI 598

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BLAST of CmoCh18G013460 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 2.2e-209
Identity = 378/599 (63.11%), Postives = 455/599 (75.96%), Query Frame = 0


Query: 1   MGCRRLNETHPAANPRRGPLSVLSLL------SLILILPAVSPTSLL------GLRT--- 60
           MG  RL  + P+   ++ P + L  L       LIL   AV    ++      G+R    
Sbjct: 1   MGYDRLGPSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDS 60

Query: 61  -SSDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNE 120
             ++PKL RKPT+AIS+ C++S  P LC+D+LLDFPGSL+A+E +L+HISFN TL   ++
Sbjct: 61  GKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSK 120

Query: 121 ALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDD 180
           ALY+SS ++   M   +RSAYD+CLELLDDSV ALTR+  S+   S D        S  D
Sbjct: 121 ALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGD-------ESHSD 180

Query: 181 VITWLSAALTNQDTCTDGFSQL---SGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFS 240
           V+TWLS+A+TN DTCTDGF ++    G VKDQ+   + +LSE+VSNCLA+F+G V+ D S
Sbjct: 181 VMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVK-DLS 240

Query: 241 GVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIA 300
           GVP+ N R+L+   E E+     P W+ R +R LL  P  A+QADI VSKDG+GT KTIA
Sbjct: 241 GVPVVNNRKLLGTEETEE----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIA 300

Query: 301 EAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVT 360
           EAIKKAPE S+R+ +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI+GGKSI D++T
Sbjct: 301 EAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLT 360

Query: 361 TFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHS 420
           TFHTA+FA +GAG I+RDMTF N+AGPA+HQAVALRV  DHAVVYRCN+IGYQD LYVHS
Sbjct: 361 TFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHS 420

Query: 421 NRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIH 480
           NRQFFREC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRKDPNQNTGISIH
Sbjct: 421 NRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIH 480

Query: 481 ACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTL 540
           AC++LATPDL  +S  + PTYLGRPWKLYSRVVYM S MG H+ PRGWLEWN  FALD+L
Sbjct: 481 ACKLLATPDL-EASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSL 540

Query: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLN 581
           YYGEYMN G G  +GQRV WPGY VITS VEAS+FTVAQFI GSSWLPSTGV+F  GL+
Sbjct: 541 YYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

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BLAST of CmoCh18G013460 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 2.3e-118
Identity = 218/466 (46.78%), Postives = 293/466 (62.88%), Query Frame = 0


Query: 110 SHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWL 169
           S  ++   D  L +A   CL+LLD S   L  S    A  +   +       S D+ TWL
Sbjct: 75  SQFANAFGDFRLANAISDCLDLLDFSADELNWSLS--ASQNQKGKNNSTGKLSSDLRTWL 134

Query: 170 SAALTNQDTCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKR 229
           SAAL NQDTC++GF   +  V+  ++  L  ++ LV   L                    
Sbjct: 135 SAALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLVQELLT------------------- 194

Query: 230 RLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPE 289
           ++   + ++  +G  P W+  ++R+L  L    +  D +V++DG G    + +A+  AP+
Sbjct: 195 QVHPNSNQQGPNGQIPSWVKTKDRKL--LQADGVSVDAIVAQDGTGNFTNVTDAVLAAPD 254

Query: 290 FSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFA 349
           +S R+ +IY+  G Y+E N+++ +KK NLM +GDG   T+ISG +S  D  TTF +A+FA
Sbjct: 255 YSMRRYVIYIKRGTYKE-NVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSATFA 314

Query: 350 VSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFREC 409
           VSG G I RD+TF N AGP +HQAVALR  +D +V YRCN+ GYQDTLY H+ RQF+R+C
Sbjct: 315 VSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYRDC 374

Query: 410 DIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATP 469
            I GTVDFIFG+A VVFQNC I A+K +  QKN+ITAQ RKDPN+ TGISI  C I A  
Sbjct: 375 KISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITADS 434

Query: 470 DLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNY 529
           DL ++S  + PTYLGRPWKLYSR V MQS++   + P GWLEWN  FAL++L+YGEYMNY
Sbjct: 435 DLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYMNY 494

Query: 530 GPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAF 576
           GPG  +G RV WPGY+V     +A  +TVAQFI G+ WLPSTGV +
Sbjct: 495 GPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKY 516

[Close]

BLAST of CmoCh18G013460 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.7e-113
Identity = 242/573 (42.23%), Postives = 342/573 (59.69%), Query Frame = 0


Query: 15  PRRGPLSVLSLLSLILILPAV-SPTSLLGLRTSSDPKLPRKPTRAISK-ACARSRLPALC 74
           P++     L+L + +L++ AV    + +  R +S      +P  AI K +C+ +R P LC
Sbjct: 24  PKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDN-GNEPHHAILKSSCSSTRYPDLC 83

Query: 75  VDSLLDFPGSLS--ANERDLVHISFNLTLHHLNEALYS-SSHLSSLHMDLPLRSAYDACL 134
             ++   P +     +++D++ +S N+T   +    +     L   ++    + A   CL
Sbjct: 84  FSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCL 143

Query: 135 ELLDDSVHALTRSFHSLAP-PSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLSG 194
           E +D+++  L ++   L   P+  S    L+  +DD+ T +SAA+TNQ TC DGFS    
Sbjct: 144 ETIDETLDELHKAVEDLEEYPNKKS----LSQHADDLKTLMSAAMTNQGTCLDGFSHDDA 203

Query: 195 N--VKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPG 254
           N  V+D ++D   ++ ++ SN LA+       D   +   N R+L+      D    +P 
Sbjct: 204 NKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETSTVD---GWPA 263

Query: 255 WMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEE 314
           W+S  +RRL  L   ++  ++VV+ DG+G  KT+A ++  AP+  T++ II + AG Y E
Sbjct: 264 WLSTGDRRL--LQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVYRE 323

Query: 315 KNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWA 374
            N++V +K  N+MF+GDG+ +T+I+G +++ D  TTF +A+ AV G G + RD+TF N A
Sbjct: 324 -NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNTA 383

Query: 375 GPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVF 434
           GP++HQAVALRV AD +  Y C+++ YQDTLYVHSNRQFF  C I GTVDFIFGNAA V 
Sbjct: 384 GPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVL 443

Query: 435 QNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRP 494
           QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI AT DL      + PTYLGRP
Sbjct: 444 QNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDL-KPVQGSFPTYLGRP 503

Query: 495 WKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRV 554
           WK YSR V MQS +   +HP GW EW+  FAL+TL+YGE+ N G G     RV W G+RV
Sbjct: 504 WKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRV 563

Query: 555 ITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 580
           ITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 564 ITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of CmoCh18G013460 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 2.2e-113
Identity = 216/452 (47.79%), Postives = 281/452 (62.17%), Query Frame = 0


Query: 128 CLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLS 187
           C++LLD +   L  S+   A  S + +         D+ TW+SAAL+NQDTC DGF   +
Sbjct: 84  CVDLLDSAAEEL--SWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTN 143

Query: 188 GNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGW 247
           G +K  +   L  +   V N L +            P     +     +       FP W
Sbjct: 144 GIIKKIVAGGLSKVGTTVRNLLTMVHSP-----PSKPKPKPIKAQTMTKAHSGFSKFPSW 203

Query: 248 MSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEK 307
           +   +R+LL        AD VV+ DG G   TI++A+  AP++ST++ +I+V  G Y E 
Sbjct: 204 VKPGDRKLLQTD-NITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVE- 263

Query: 308 NLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAG 367
           N+++ +KK N+M VGDG   TVI+G +S  D  TTF +A+FAVSG G I RD+TF N AG
Sbjct: 264 NVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAG 323

Query: 368 PARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQ 427
           P +HQAVA+R   D  V YRC + GYQDTLY HS RQFFREC I GTVDFIFG+A  VFQ
Sbjct: 324 PEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQ 383

Query: 428 NCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPW 487
           +C I A++ +  QKN+ITAQ RKDPN+ TG +I    I A  DL  +   T  TYLGRPW
Sbjct: 384 SCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLN-TTATYLGRPW 443

Query: 488 KLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVI 547
           KLYSR V+MQ+YM   ++P GWLEWN  FALDTLYYGEYMN GPG ++ +RV WPGY V+
Sbjct: 444 KLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVL 503

Query: 548 TSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 580
            +  EA+ FTV+Q I G+ WLPSTG+ F+ GL
Sbjct: 504 NTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

[Close]

BLAST of CmoCh18G013460 vs. ExPASy TrEMBL
Match: A0A6J1G0F6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1)

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0


Query: 1   MGCRRLNETHPAANPRRGPLSVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAIS 60
           MGCRRLNETHPAANPRRGPLSVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAIS
Sbjct: 1   MGCRRLNETHPAANPRRGPLSVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAIS 60

Query: 61  KACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLP 120
           KACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLP
Sbjct: 61  KACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLP 120

Query: 121 LRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCT 180
           LRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCT
Sbjct: 121 LRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCT 180

Query: 181 DGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDD 240
           DGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDD
Sbjct: 181 DGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDD 240

Query: 241 SGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVM 300
           SGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVM
Sbjct: 241 SGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVM 300

Query: 301 AGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDM 360
           AGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDM
Sbjct: 301 AGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDM 360

Query: 361 TFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFG 420
           TFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFG
Sbjct: 361 TFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFG 420

Query: 421 NAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNP 480
           NAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNP
Sbjct: 421 NAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNP 480

Query: 481 TYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVT 540
           TYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVT
Sbjct: 481 TYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVT 540

Query: 541 WPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           WPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL
Sbjct: 541 WPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 581

[Close]

BLAST of CmoCh18G013460 vs. ExPASy TrEMBL
Match: A0A6J1HX67 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467471 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 561/584 (96.06%), Postives = 566/584 (96.92%), Query Frame = 0


Query: 1   MGCRRLNETHPAANPRRGPLSVLSLLSLILIL---PAVSPTSLLGLRTSSDPKLPRKPTR 60
           MGCRRLNETHPAA P RGPL +LSLLSLILIL    A SP SLL LRT S P LPRKPTR
Sbjct: 1   MGCRRLNETHPAAKPMRGPLFLLSLLSLILILILPAAASPASLLRLRTPSHPNLPRKPTR 60

Query: 61  AISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHM 120
           AISKACARSRLPALCVDSLLDFPGSLSANE+DLVHISFNLTLHHLN+ALYSSSHLSSLHM
Sbjct: 61  AISKACARSRLPALCVDSLLDFPGSLSANEQDLVHISFNLTLHHLNQALYSSSHLSSLHM 120

Query: 121 DLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQD 180
           DLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLA SSDDVITWLSAALTNQD
Sbjct: 121 DLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLA-SSDDVITWLSAALTNQD 180

Query: 181 TCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEK 240
           TCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEK
Sbjct: 181 TCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEK 240

Query: 241 EDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTII 300
           E DSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTII
Sbjct: 241 EYDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTII 300

Query: 301 YVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIIL 360
           YVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIIL
Sbjct: 301 YVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIIL 360

Query: 361 RDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDF 420
           RD+TFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDF
Sbjct: 361 RDVTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDF 420

Query: 421 IFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTA 480
           IFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHA RILATPDLAS ST 
Sbjct: 421 IFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHASRILATPDLASPSTT 480

Query: 481 TNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQ 540
           TNPTYLGRPWKLYSRVVYMQSY+GGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQ
Sbjct: 481 TNPTYLGRPWKLYSRVVYMQSYIGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQ 540

Query: 541 RVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           RVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL
Sbjct: 541 RVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 583

[Close]

BLAST of CmoCh18G013460 vs. ExPASy TrEMBL
Match: A0A6J1E487 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.3e-278
Identity = 497/602 (82.56%), Postives = 535/602 (88.87%), Query Frame = 0


Query: 1   MGCRRLNETHP----------------AANPRRGPLSVLSLLSLILIL-PAVSPTSLLGL 60
           MG  RLNET P                + NPRR  L ++SLLSLILI+  AVS T L+GL
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTS----SDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTL 120
           RT+    SDP LPRKPT+AISKAC+R+R P LCV+SLLDFPGSL+ANE+DLVHISFN+TL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 HHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLA 180
            HLN+ALY SS +SSL MDL +RSAYDACLELLDDSV ALTRS  S+AP SS+  P  L 
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRL- 180

Query: 181 SSSDDVITWLSAALTNQDTCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRND 240
            SSDDVITWLSAALTNQDTCTDGFSQLSG+VKDQM DKLHNLSELVSNCLALFSGSV +D
Sbjct: 181 GSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSD 240

Query: 241 FSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKT 300
           FSGVPIQNKRRLMNA E ED SGNFPGWMSRRERRLLTLPVGALQADIVVS++G+GTVKT
Sbjct: 241 FSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKT 300

Query: 301 IAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDN 360
           IAEAIKKAP++S R+TIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISGGKSIFDN
Sbjct: 301 IAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDN 360

Query: 361 VTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYV 420
           VTTFHTASFA +GAGIILRDMTF NWAGP RHQAVALRV  DHAVVYRCN+IGYQDTLYV
Sbjct: 361 VTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYV 420

Query: 421 HSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGIS 480
           HSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAM+LQKNT+TAQNRKDPNQNTGIS
Sbjct: 421 HSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGIS 480

Query: 481 IHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALD 540
           IHACRILA  DLASS+ ++NPTYLGRPWKLYSRVV+M SY+G HVHPRGWLEWNATFALD
Sbjct: 481 IHACRILAASDLASSN-SSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 582
           TLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEAS+FTVAQFIYGSSWLPSTGVAFLGGL
Sbjct: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600

[Close]

BLAST of CmoCh18G013460 vs. ExPASy TrEMBL
Match: A0A6J1J930 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.3e-277
Identity = 495/602 (82.23%), Postives = 534/602 (88.70%), Query Frame = 0


Query: 1   MGCRRLNETHP----------------AANPRRGPLSVLSLLSLILIL-PAVSPTSLLGL 60
           M   RLN+T P                + NPRR  L ++SLLSLILI+  AVS T L+GL
Sbjct: 1   MAYGRLNQTDPGGTSSDAPSQLSHHAVSPNPRRRRLILISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTS----SDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTL 120
           RT+    SDP LPRKPT+AISKAC+R+R P LCV+SLLDFPGSL+ANE+DLVHISFN+TL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 HHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLA 180
            HLN+ALY SS +SSL MDL +RSAYDACLELLDDSV ALTRS  S+AP SS+  P  L 
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRL- 180

Query: 181 SSSDDVITWLSAALTNQDTCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRND 240
            SSDDVITWLSAALTNQDTCTDGFSQLSG+VKDQM DKLHNLSELVSNCLALFSGSV +D
Sbjct: 181 GSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSD 240

Query: 241 FSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKT 300
           FSGVPIQNKRRLMNA E ED SG+FPGWMSRRERRLLTLPVGALQADIVVS++G+GTVKT
Sbjct: 241 FSGVPIQNKRRLMNAGENEDTSGSFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKT 300

Query: 301 IAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDN 360
           IAEAIKKAP++S R+TIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISGGKSIFDN
Sbjct: 301 IAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKCNLMFVGDGKGKTVISGGKSIFDN 360

Query: 361 VTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYV 420
           VTTFHTASFA +GAGIILRDMTF NWAGP RHQAVALRV  DHAVVYRCN+IGYQDTLYV
Sbjct: 361 VTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYV 420

Query: 421 HSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGIS 480
           HSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAM+LQKNTITAQNRKDPNQNTGIS
Sbjct: 421 HSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGIS 480

Query: 481 IHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALD 540
           IHACRILA  DLASS+ ++NPTYLGRPWKLYSRVV+M SY+G HVHPRGWLEWNATFALD
Sbjct: 481 IHACRILAASDLASSN-SSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 582
           TLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEAS+FTVAQFIYGSSWLPSTGVAFLGGL
Sbjct: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600

[Close]

BLAST of CmoCh18G013460 vs. ExPASy TrEMBL
Match: A0A5A7U3N7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001450 PE=3 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 7.9e-271
Identity = 479/583 (82.16%), Postives = 521/583 (89.37%), Query Frame = 0


Query: 5   RLNETHPAANPRRGPLSVLSLLSLILIL-PAVSPTSLLGLRTS----SDPKLPRKPTRAI 64
           +L+  HP  N     L ++S LSL+LI+  AVS T L+GLRT+    SDP LPRKPT+AI
Sbjct: 25  QLSAHHPTPNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAI 84

Query: 65  SKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDL 124
           SKAC+R+R P LCV+SLLDFPGSL+ANE+DLVHISFN+TL HLN+ALYSSS +SSL MDL
Sbjct: 85  SKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDL 144

Query: 125 PLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTC 184
            +RSAYDACLELLDDS+ A++RS  S+AP SS S P  L  SS+D+ITWLSAALTNQDTC
Sbjct: 145 RVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRL-GSSEDIITWLSAALTNQDTC 204

Query: 185 TDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLM-NAAEKE 244
           TDGFS+LSG VK+QM DKLHNLSELVSNCLALFSGS  +DF+GVPIQNKRRLM    E E
Sbjct: 205 TDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENE 264

Query: 245 DDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIY 304
           D SGNFP WM  RERRLLTLPVG LQADIVVS++GNGTVKTIAEAIKKAP++S R+TIIY
Sbjct: 265 DSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIY 324

Query: 305 VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILR 364
           VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG KSIFDNVTTFHTASFA +GAGIILR
Sbjct: 325 VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGSKSIFDNVTTFHTASFAATGAGIILR 384

Query: 365 DMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFI 424
           DMTF NWAGP RHQAVALRV ADHAVVYRCN+IGYQDTLYVHSNRQF+RECDIYGTVDFI
Sbjct: 385 DMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFI 444

Query: 425 FGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTAT 484
           FGNAAVVFQNCSIYARKAM LQKNTITAQNRKDPNQNTGISIHACRILAT DL SS+T +
Sbjct: 445 FGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPNQNTGISIHACRILATSDLQSSNT-S 504

Query: 485 NPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQR 544
           NPTYLGRPWKLYSRVV+M SY+GGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQR
Sbjct: 505 NPTYLGRPWKLYSRVVFMLSYIGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQR 564

Query: 545 VTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           VTWPGYRVITS VEAS+FTVAQFIYGSSWLPSTGVAFLGGLN+
Sbjct: 565 VTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 605

[Close]

BLAST of CmoCh18G013460 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 753.8 bits (1945), Expect = 1.0e-217
Identity = 386/564 (68.44%), Postives = 451/564 (79.96%), Query Frame = 0


Query: 21  SVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAISKACARSRLPALCVDSLLDFP 80
           S++  +SLIL     +          S P L RKP++AISKAC  +R P LCVDSL+DFP
Sbjct: 49  SIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFP 108

Query: 81  GSLSA-NERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHAL 140
           GSL+A + +DL+H++ N+TLHH + ALYSS+ LS + M    RSAYD+C+ELLDDSV AL
Sbjct: 109 GSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDAL 168

Query: 141 TRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQL-SGNVKDQMTDKL 200
           +R+  S+   SS ++P        DV TWLSAALTN DTCT+GF  +  G VKD MT  L
Sbjct: 169 SRALSSVV--SSSAKP-------QDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAAL 228

Query: 201 HNLSELVSNCLALFSGS-VRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLT 260
            NLSELVSNCLA+FS S   +DF+GVPIQN RRL+   E+E+    FP WM  +ER +L 
Sbjct: 229 QNLSELVSNCLAIFSASHDGDDFAGVPIQN-RRLLGVEEREE---KFPRWMRPKEREILE 288

Query: 261 LPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTN 320
           +PV  +QADI+VSKDGNGT KTI+EAIKKAP+ STR+ IIYV AGRYEE NLKVGRKK N
Sbjct: 289 MPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKIN 348

Query: 321 LMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALR 380
           LMFVGDGKGKTVISGGKSIFDN+TTFHTASFA +GAG I RD+TF NWAGPA+HQAVALR
Sbjct: 349 LMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALR 408

Query: 381 VSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAM 440
           + ADHAV+YRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVV QNCSIYARK M
Sbjct: 409 IGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPM 468

Query: 441 ELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQ 500
           + QKNTITAQNRKDPNQNTGISIHA R+LA  DL +++ +T  TYLGRPWKL+SR VYM 
Sbjct: 469 DFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQ-TYLGRPWKLFSRTVYMM 528

Query: 501 SYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFT 560
           SY+GGHVH RGWLEWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI S  EA++FT
Sbjct: 529 SYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFT 588

Query: 561 VAQFIYGSSWLPSTGVAFLGGLNL 582
           VA+FIYGSSWLPSTGV+FL GL++
Sbjct: 589 VAEFIYGSSWLPSTGVSFLAGLSI 598

[Close]

BLAST of CmoCh18G013460 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 729.9 bits (1883), Expect = 1.6e-210
Identity = 378/599 (63.11%), Postives = 455/599 (75.96%), Query Frame = 0


Query: 1   MGCRRLNETHPAANPRRGPLSVLSLL------SLILILPAVSPTSLL------GLRT--- 60
           MG  RL  + P+   ++ P + L  L       LIL   AV    ++      G+R    
Sbjct: 1   MGYDRLGPSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDS 60

Query: 61  -SSDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNE 120
             ++PKL RKPT+AIS+ C++S  P LC+D+LLDFPGSL+A+E +L+HISFN TL   ++
Sbjct: 61  GKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSK 120

Query: 121 ALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDD 180
           ALY+SS ++   M   +RSAYD+CLELLDDSV ALTR+  S+   S D        S  D
Sbjct: 121 ALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGD-------ESHSD 180

Query: 181 VITWLSAALTNQDTCTDGFSQL---SGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFS 240
           V+TWLS+A+TN DTCTDGF ++    G VKDQ+   + +LSE+VSNCLA+F+G V+ D S
Sbjct: 181 VMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVK-DLS 240

Query: 241 GVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIA 300
           GVP+ N R+L+   E E+     P W+ R +R LL  P  A+QADI VSKDG+GT KTIA
Sbjct: 241 GVPVVNNRKLLGTEETEE----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIA 300

Query: 301 EAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVT 360
           EAIKKAPE S+R+ +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI+GGKSI D++T
Sbjct: 301 EAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLT 360

Query: 361 TFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHS 420
           TFHTA+FA +GAG I+RDMTF N+AGPA+HQAVALRV  DHAVVYRCN+IGYQD LYVHS
Sbjct: 361 TFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHS 420

Query: 421 NRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIH 480
           NRQFFREC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRKDPNQNTGISIH
Sbjct: 421 NRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIH 480

Query: 481 ACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTL 540
           AC++LATPDL  +S  + PTYLGRPWKLYSRVVYM S MG H+ PRGWLEWN  FALD+L
Sbjct: 481 ACKLLATPDL-EASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSL 540

Query: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLN 581
           YYGEYMN G G  +GQRV WPGY VITS VEAS+FTVAQFI GSSWLPSTGV+F  GL+
Sbjct: 541 YYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

[Close]

BLAST of CmoCh18G013460 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 411.0 bits (1055), Expect = 1.6e-114
Identity = 216/452 (47.79%), Postives = 281/452 (62.17%), Query Frame = 0


Query: 128 CLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLS 187
           C++LLD +   L  S+   A  S + +         D+ TW+SAAL+NQDTC DGF   +
Sbjct: 84  CVDLLDSAAEEL--SWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTN 143

Query: 188 GNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGW 247
           G +K  +   L  +   V N L +            P     +     +       FP W
Sbjct: 144 GIIKKIVAGGLSKVGTTVRNLLTMVHSP-----PSKPKPKPIKAQTMTKAHSGFSKFPSW 203

Query: 248 MSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEK 307
           +   +R+LL        AD VV+ DG G   TI++A+  AP++ST++ +I+V  G Y E 
Sbjct: 204 VKPGDRKLLQTD-NITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVE- 263

Query: 308 NLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAG 367
           N+++ +KK N+M VGDG   TVI+G +S  D  TTF +A+FAVSG G I RD+TF N AG
Sbjct: 264 NVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAG 323

Query: 368 PARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQ 427
           P +HQAVA+R   D  V YRC + GYQDTLY HS RQFFREC I GTVDFIFG+A  VFQ
Sbjct: 324 PEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQ 383

Query: 428 NCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPW 487
           +C I A++ +  QKN+ITAQ RKDPN+ TG +I    I A  DL  +   T  TYLGRPW
Sbjct: 384 SCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLN-TTATYLGRPW 443

Query: 488 KLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVI 547
           KLYSR V+MQ+YM   ++P GWLEWN  FALDTLYYGEYMN GPG ++ +RV WPGY V+
Sbjct: 444 KLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVL 503

Query: 548 TSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 580
            +  EA+ FTV+Q I G+ WLPSTG+ F+ GL
Sbjct: 504 NTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

[Close]

BLAST of CmoCh18G013460 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 408.7 bits (1049), Expect = 7.9e-114
Identity = 226/532 (42.48%), Postives = 307/532 (57.71%), Query Frame = 0


Query: 59  ISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMD 118
           +   C+ +  P LC  ++    G    ++++++  S NLT   +    ++   L +    
Sbjct: 62  LKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKG 121

Query: 119 LPLR--SAYDACLELLD---DSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAAL 178
           L  R  +A   CLE +D   D +H      H      S      L   +DD+ T +S+A+
Sbjct: 122 LTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKS------LRKHADDLKTLISSAI 181

Query: 179 TNQDTCTDGFS--QLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQ----- 238
           TNQ TC DGFS       V+  +     ++  + SN LA+       D +   ++     
Sbjct: 182 TNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSST 241

Query: 239 ---NKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEA 298
              N  R +     + DS  +P W+S  +RRL  L    ++AD  V+ DG+G   T+A A
Sbjct: 242 FTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRL--LQGSTIKADATVADDGSGDFTTVAAA 301

Query: 299 IKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTF 358
           +  APE S ++ +I++ AG Y E N++V +KKTN+MF+GDG+GKT+I+G +++ D  TTF
Sbjct: 302 VAAAPEKSNKRFVIHIKAGVYRE-NVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTF 361

Query: 359 HTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNR 418
           H+A+ A  G   + RD+TF N AGP++HQAVALRV +D +  Y+C++  YQDTLYVHSNR
Sbjct: 362 HSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNR 421

Query: 419 QFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHAC 478
           QFF +C I GTVDFIFGNAA V Q+C I AR+    QKN +TAQ R DPNQNTGI I  C
Sbjct: 422 QFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNC 481

Query: 479 RILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYY 538
           RI  T DL +    T PTYLGRPWK YSR V MQS +   + P GW EW+ +FALDTL Y
Sbjct: 482 RIGGTSDLLAVK-GTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTY 541

Query: 539 GEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAF 576
            EY+N G G     RV W GY+VITS  EA  FT  QFI G  WL STG  F
Sbjct: 542 REYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

[Close]

BLAST of CmoCh18G013460 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 404.4 bits (1038), Expect = 1.5e-112
Identity = 238/583 (40.82%), Postives = 330/583 (56.60%), Query Frame = 0


Query: 20  LSVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAISKACARSRLPALCVDSLLDF 79
           L +LS    +L + AV+  S    + +    L       +  +C+ +R P LC+ +++  
Sbjct: 20  LVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTA 79

Query: 80  PGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLR--SAYDACLELLD---D 139
            G    +++D++  S NLT+  +    ++   L      L  R  +A   CLE +D   D
Sbjct: 80  GGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLD 139

Query: 140 SVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFS--QLSGNVKD 199
            +H      H L P         L   + D+ T +S+A+TNQ+TC DGFS       V+ 
Sbjct: 140 ELHETVEDLH-LYPTKK-----TLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 199

Query: 200 QMTDKLHNLSELVSNCLALFSGSVRNDFSGVP-----IQNKRRLMN-----------AAE 259
            +     ++  + SN LA+       D +          N R+L             A  
Sbjct: 200 ALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGA 259

Query: 260 KEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTI 319
            E DS  +P W+S  +RRL  L    ++AD  V+ DG+GT KT+A A+  APE S ++ +
Sbjct: 260 GELDSEGWPTWLSAGDRRL--LQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYV 319

Query: 320 IYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGII 379
           I++ AG Y E N++V +KK N+MF+GDG+ +T+I+G +++ D  TTFH+A+ A  G   +
Sbjct: 320 IHIKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFL 379

Query: 380 LRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVD 439
            RD+TF N AGP++HQAVALRV +D +  Y C+++ YQDTLYVHSNRQFF +C I GTVD
Sbjct: 380 ARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVD 439

Query: 440 FIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSST 499
           FIFGNAAVV Q+C I+AR+    QKN +TAQ R DPNQNTGI I  CRI AT DL  S  
Sbjct: 440 FIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL-QSVK 499

Query: 500 ATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVG 559
            + PTYLGRPWK YS+ V MQS +   + P GW EW  TFAL+TL Y EY N G G    
Sbjct: 500 GSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTA 559

Query: 560 QRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 580
            RV W G++VIT+  EA ++T  QFI G  WL STG  F  GL
Sbjct: 560 NRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

The following BLAST results are available for this feature:

BLAST of CmoCh18G013460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M3B0	1.4e-216	68.44	Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3...	[more]
Q9FK05	2.2e-209	63.11	Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3...	[more]
Q43062	2.3e-118	46.78	Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=...	[more]
P83948	1.7e-113	42.23	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]
Q9LXK7	2.2e-113	47.79	Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmoCh18G013460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G0F6	0.0e+00	100.00	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1	[more]
A0A6J1HX67	0.0e+00	96.06	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467471 PE=3 SV=1	[more]
A0A6J1E487	1.3e-278	82.56	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1	[more]
A0A6J1J930	3.3e-277	82.23	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1	[more]
A0A5A7U3N7	7.9e-271	82.16	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00145...	[more]

BLAST of CmoCh18G013460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49220.1	1.0e-217	68.44	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G53370.1	1.6e-210	63.11	pectin methylesterase PCR fragment F	[more]
AT3G43270.1	1.6e-114	47.79	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G53830.1	7.9e-114	42.48	pectin methylesterase 2	[more]
AT3G14310.1	1.5e-112	40.82	pectin methylesterase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 54..211 e-value: 2.3E-38 score: 143.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 58..211 e-value: 1.1E-29 score: 103.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 40..212 e-value: 3.1E-33 score: 112.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 56..214 e-value: 1.5E-33 score: 117.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 58..213
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 266..564 e-value: 3.5E-140 score: 465.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 260..579 e-value: 1.4E-115 score: 388.0
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 17..579
None	No IPR available	PANTHER	PTHR31707:SF222	PECTINESTERASE	coord: 17..579
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 62..213 e-value: 1.74658E-41 score: 145.274
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 411..420
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 262..577

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh18G013460.1	CmoCh18G013460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity