CmoCh18G013040 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh18G013040
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	DNA-directed primase/polymerase protein
Location	Cmo_Chr18: 12497733 .. 12500953 (+)
RNA-Seq Expression	CmoCh18G013040
Synteny	CmoCh18G013040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGAAGGACGACGTCGATCGGTCGTTTGAATGCTTCATGTGCGGCGTTTCTCATCCTTGTAAGTTCTGTTTCTCTAATTTTAATTCGAGTTTCTACAACCTACGCAGCCATGCGAGCTCTGCGAAACAATGTACTGCGAATCTCGTTTTTAGACAATGAATTTCACTGTGAAGCATTTGTAATTATTGTAGAAAAACTACTAGATTTTGTAAACTGGAATTACGATAGAGGAGCAATGTTATAGGTCGAGTTTCTGCTGTGATTTGGTGATCAGCATGGATGTTCTTTGTTTCTGTATTTTCTGCTTCTGATTATGTGCGGTTATCCATCCTGTTTCTGTTCAGAATCTTCCGTGAGAGGAAAAAAGAAAGAAAAATTACATTGAAGCCTGAGAGTTCAGCAAACTTTGAGACTCCAGTTTCTGATTCAGTTAAGGAAATACAGGACAATGCAACGAACGGCCAAGATTTCATGGAAAAAGTAAGTCGTTGTTTTCTGGATGGCGGAGTTTGCCTGAATTCCATGAACTTATTCTTCATATGCTTATTCAAGTATTTCAGCTGTCTAATGAAGTTCAGAGTTCGTTGAGCTAATGATGAGCCTGAAACAGCTTGATTGTTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCGTTGCTTGACAAAACTATAGTTATCACGATTCAGTTTGGTTTGACAAGTAAAGCAAAAGGTTCTAGTTATAAGAAGTTCTCGCCAGTCTTATTTTATAATTCACCTAGAGGCGTACCTCCGAGGAGGCCATCTAGTTTGTGGAGATTATTGCTTGAAATTCGTGTCGAGCTTTCTGAACAAAGTAGATTCAAGTTTAGGTATATTTAGGTTTCATCAAATCTCTCCCATTCCTCTTCCTGTTTCACCATCATTCCACTTCTCGATGATTCCATTTGACATTTCTGGTATCTTTTAAGTAGTGAAGTTTGCAATGAAGTTTGCTGTGAAGTTTGGACCACATTTCCTAGACAAGAAGAAGCAATGAAAGTTTGCAAGAGAGCACACAGATGTTCATATTTTAGATATCAAGATCACTTTAATGAACAAAGGAGATTTCTAGTTTCATCACACAGCAAGTTTTGGCAATGTTGTCATCAAATTTTAATAATACTTCGAGTAGAAAAATTATTTTTCCATCAGCCTAGCAAACGCTTCTGATGTCGTTCTAACTACGTTCTTAATTAATTTCAGGTACAAAAGCATGGATTCAAAATTTAGCCACCATTAAATTGATTCAAGAGGTGAGCTGAAAAAGTTCGAGTCAATGTGCCGATCATGTAATTGAATCTGTGCGAATCGTGTTCTCTTTTCTTTTTTCTACTGCACTTTCTCACTGTTCTATTCCAACGTTCAGGTTGCTCCGCGTCATGTTTACTTTGATATGGAGTACAAGATAAAAATCAGCGCGGGAAAAATTGGAGACGTAATGGTTGATTCCTAGATATCAGTTGTTCTAGAGGCCTCAAATTGAAAGTATTCGATTCAAGGAAGTTTTGACTGGATACTAGAGCTTGATTCCTCAAACAAGGTATATTTAGCAACAATTGGATTTAGGCTTCATCTTCTTGTCACTTGTGTTATTTCTTTGTTATCTCTTGAAATGTATACTTAATAGGTGAAACTAGGTTCTTGTAATTTCTTTTCTTTTCTTTTATTTTTTAGACATCGCATAGCTCAAATTTTCTTGGTATTAGTATAACATATCATTTTTATCCTTTTTATTAGTAACAAGTTCATAATAGTTAACAGGATTATTTCTGGTAATTCTACAGGAATATGATTACTTCATTTCTTAAAGAAAATTATTTTTGCTAGACAAGTTCAGATTAAAAATATATATATAATTTTGGTTTCCAACAGAAAAGAGGAAGTTGAAGGCACTTCGTAGTCATCATTATTTAGAAATTTTGCCAAAACTTTTCCAATATCGGAGAAATTGTTTTACTCCACCTTTCAAGAAAGTAGTTGCGCAGGATACATTATTCTGAGAAGGATCCTATTATTTACCTTGTACATATTTAATCGTAGTGTGAGGAAGACATGTTCATGGCATCCTTGGTCTGCAACGTCGATGCTGAGTGTGAGAAACTTCTGGTTTGCGAAACGGAATTACACTGCGTAAAGACATTGCAATTTGAAATGGAGGTACGATCAAGCTGAGAATCCATTTAGTTATGAAGCTGCTTTGAACAGTCCATGACATGTCGATTATACCTTTCAAATTGAATTATCTTATAAATCCAAATGTCTCGTACCTTTATGTTTGATTTTAGTTATTCAACATTTGTTTACCTTTGAGGTTCTCTGGCCGCAGGAGATATGTAATTTTGGACGGTTTGTTGAATCAGCTGCCTCAACCGGAAATGTATCAGGTAATTTACCTGTATGAAAACCCCATGGGATAGACAAGTTTTGTACTTCGTGCGATCTACTTTCTCTGGTTGTTCTTTTTCTTTAAATCATCTGAGAATTTACTCATATCATCTTTCTTGCATGCAAAACATTGATCATCCATGTTTTCATCGTTCAGTGATGTATGTTGTCGACCTTACGATGGCTGCCTATTACCAAAGTGTCACGATCCAGATTGTGGAGGTGCTCAAATAAAACTTAATTGTCAACTTTTTTTCGGAAAAATATTTATTTGCAGCCATTGCTTGATTGAACTTATCACCCTACAGGTTACCGATCTCCACTGCGTCCAAGTCCAATTGATGCCATTCCTAATCCTTGGGTCTCCACGGATTCAGAACAGATATCAAATGACATAGAATCGTCCAACTGATGCCATCTTCGTGCATGCGTTTCCAACTCGCGAGCAGCTAGGTTTCGGATCCAGGGTAAGCAAAAATCCCTGAACACCTAATTATTTTCTGAACTCTTTGATATTGTCCATAGTTAATTGAGCTTAAGAGTCAAATTGTTCTGCTCCCCTTTCAAAGCTCCGCACCATTAGAGAGTCAGTTTTATTAACGCAAATAGGATAAATTTCTATCTCGATCGCC

mRNA sequence

GTGAAGGACGACGTCGATCGGTCGTTTGAATGCTTCATGTGCGGCGTTTCTCATCCTTAATCTTCCGACAATGCAACGAACGGCCAAGATTTCATGGAAAAAGTACAAAAGCATGGATTCAAAATTTAGCCACCATTAAATTGATTCAAGAGGTTGCTCCGCGTCATGTTTACTTTGATATGGAGTACAAGATAAAAATCAGCGCGGGAAAAATTGGAGACCTTGATTCCTCAAACAAGTGTGAGGAAGACATGTTCATGGCATCCTTGGTCTGCAACGTCGATGCTGAGTGTGAGAAACTTCTGGTTTGCGAAACGGAATTACACTGCGTAAAGACATTGCAATTTGAAATGGAGGAGATATGTAATTTTGGACGGTTTGTTGAATCAGCTGCCTCAACCGGAAATGTATCAGTGATGTATGTTGTCGACCTTACGATGGCTGCCTATTACCAAAGTGTCACGATCCAGATTGTGGAGGTTACCGATCTCCACTGCGTCCAAGTCCAATTGATGCCATTCCTAATCCTTGGGTCTCCACGGATTCAGAACAGATATCAAATGACATAGAATCGTCCAACTGATGCCATCTTCGTGCATGCGTTTCCAACTCGCGAGCAGCTAGGTTTCGGATCCAGGGTAAGCAAAAATCCCTGAACACCTAATTATTTTCTGAACTCTTTGATATTGTCCATAGTTAATTGAGCTTAAGAGTCAAATTGTTCTGCTCCCCTTTCAAAGCTCCGCACCATTAGAGAGTCAGTTTTATTAACGCAAATAGGATAAATTTCTATCTCGATCGCC

Coding sequence (CDS)

ATGCTTCATGTGCGGCGTTTCTCATCCTTAATCTTCCGACAATGCAACGAACGGCCAAGATTTCATGGAAAAAGTACAAAAGCATGGATTCAAAATTTAGCCACCATTAAATTGATTCAAGAGGTTGCTCCGCGTCATGTTTACTTTGATATGGAGTACAAGATAAAAATCAGCGCGGGAAAAATTGGAGACCTTGATTCCTCAAACAAGTGTGAGGAAGACATGTTCATGGCATCCTTGGTCTGCAACGTCGATGCTGAGTGTGAGAAACTTCTGGTTTGCGAAACGGAATTACACTGCGTAAAGACATTGCAATTTGAAATGGAGGAGATATGTAATTTTGGACGGTTTGTTGAATCAGCTGCCTCAACCGGAAATGTATCAGTGATGTATGTTGTCGACCTTACGATGGCTGCCTATTACCAAAGTGTCACGATCCAGATTGTGGAGGTTACCGATCTCCACTGCGTCCAAGTCCAATTGATGCCATTCCTAATCCTTGGGTCTCCACGGATTCAGAACAGATATCAAATGACATAG

Protein sequence

MLHVRRFSSLIFRQCNERPRFHGKSTKAWIQNLATIKLIQEVAPRHVYFDMEYKIKISAGKIGDLDSSNKCEEDMFMASLVCNVDAECEKLLVCETELHCVKTLQFEMEEICNFGRFVESAASTGNVSVMYVVDLTMAAYYQSVTIQIVEVTDLHCVQVQLMPFLILGSPRIQNRYQMT

Homology

[Close]

BLAST of CmoCh18G013040 vs. ExPASy TrEMBL
Match: A0A1S4DSS4 (DNA-directed primase/polymerase protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484132 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 7.9e-20
Identity = 66/148 (44.59%), Postives = 78/148 (52.70%), Query Frame = 0


Query: 43  APRHVYFD---------MEYKIKISAGKIGDLDSSNK------CEEDMFMASLVCNVDAE 102
           +P H++ D             +   AGK   L  S +      CEEDMFMASL+CNVDAE
Sbjct: 303 SPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPSGRHAYTKMCEEDMFMASLICNVDAE 362

Query: 103 CEKLLVCETELHCVKTLQFEMEEICNFGR------------------------------- 143
           CEKLLVC+ +L C+KTLQFE EE CNFGR                               
Sbjct: 363 CEKLLVCKMDLDCIKTLQFETEEKCNFGRNVSFTQEAVLTGSMRDISATSCMGKSPFPDV 422

[Close]

BLAST of CmoCh18G013040 vs. ExPASy TrEMBL
Match: A0A5D3DH78 (DNA-directed primase/polymerase protein isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001190 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.1e-16
Identity = 54/101 (53.47%), Postives = 61/101 (60.40%), Query Frame = 0


Query: 75  MFMASLVCNVDAECEKLLVCETELHCVKTLQFEMEEICNFGR------------------ 134
           MFMASL+CNVDAECEKLLVC+ +L C+KTLQFE EE CNFGR                  
Sbjct: 1   MFMASLICNVDAECEKLLVCKMDLDCIKTLQFETEEKCNFGRNVSFTQEAVMTGSMRDIS 60

Query: 135 ---------------FVESAASTGNVSVMYVVDLTMAAYYQ 143
                          FV+S ASTG+V+VMYVVDL  AAYYQ
Sbjct: 61  ATSCMGKSPFPDVDKFVQSVASTGSVAVMYVVDLRSAAYYQ 101

[Close]

BLAST of CmoCh18G013040 vs. ExPASy TrEMBL
Match: A0A6J1E4L8 (DNA-directed primase/polymerase protein OS=Cucurbita moschata OX=3662 GN=LOC111430698 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 5.9e-15
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 0


Query: 71  CEEDMFMASLVCNVDAECEKLLVCETELHCVKTLQFEMEEICNFGR---FVESAASTG 126
           CEEDMFMASL+CNVDA+C+KLLVC+T+L CVKTLQFEMEE CNFGR   F + A  TG
Sbjct: 347 CEEDMFMASLICNVDADCDKLLVCKTDLDCVKTLQFEMEEKCNFGRQFSFAKEAPLTG 404

[Close]

BLAST of CmoCh18G013040 vs. ExPASy TrEMBL
Match: A0A1S3AYI4 (DNA-directed primase/polymerase protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484132 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 7.7e-15
Identity = 50/103 (48.54%), Postives = 62/103 (60.19%), Query Frame = 0


Query: 43  APRHVYFD---------MEYKIKISAGKIGDLDSSNK------CEEDMFMASLVCNVDAE 102
           +P H++ D             +   AGK   L  S +      CEEDMFMASL+CNVDAE
Sbjct: 303 SPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPSGRHAYTKMCEEDMFMASLICNVDAE 362

Query: 103 CEKLLVCETELHCVKTLQFEMEEICNFGR---FVESAASTGNV 128
           CEKLLVC+ +L C+KTLQFE EE CNFGR   F + A  TG++
Sbjct: 363 CEKLLVCKMDLDCIKTLQFETEEKCNFGRNVSFTQEAVLTGSM 405

[Close]

BLAST of CmoCh18G013040 vs. ExPASy TrEMBL
Match: A0A1S4DSQ0 (DNA-directed primase/polymerase protein isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484132 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 7.7e-15
Identity = 50/103 (48.54%), Postives = 62/103 (60.19%), Query Frame = 0


Query: 43  APRHVYFD---------MEYKIKISAGKIGDLDSSNK------CEEDMFMASLVCNVDAE 102
           +P H++ D             +   AGK   L  S +      CEEDMFMASL+CNVDAE
Sbjct: 303 SPSHLFVDNAVYSRNRCFRLALSSKAGKTSVLLPSGRHAYTKMCEEDMFMASLICNVDAE 362

Query: 103 CEKLLVCETELHCVKTLQFEMEEICNFGR---FVESAASTGNV 128
           CEKLLVC+ +L C+KTLQFE EE CNFGR   F + A  TG++
Sbjct: 363 CEKLLVCKMDLDCIKTLQFETEEKCNFGRNVSFTQEAVLTGSM 405

[Close]

BLAST of CmoCh18G013040 vs. TAIR 10
Match: AT5G52800.1 (DNA primases )

HSP 1 Score: 54.7 bits (130), Expect = 9.0e-08
Identity = 23/38 (60.53%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 72  EEDMFMASLVCNVDAECEKLLVCETELHCVKTLQFEME 110
           E D+FM SL+CNV+++CEKLLVC+ E  C+KTL F+ E
Sbjct: 358 ERDVFMTSLICNVESDCEKLLVCKMESDCMKTLCFDTE 395

[Close]

BLAST of CmoCh18G013040 vs. TAIR 10
Match: AT5G52800.2 (DNA primases )

HSP 1 Score: 54.7 bits (130), Expect = 9.0e-08
Identity = 23/38 (60.53%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 72  EEDMFMASLVCNVDAECEKLLVCETELHCVKTLQFEME 110
           E D+FM SL+CNV+++CEKLLVC+ E  C+KTL F+ E
Sbjct: 358 ERDVFMTSLICNVESDCEKLLVCKMESDCMKTLCFDTE 395

[Close]

BLAST of CmoCh18G013040 vs. TAIR 10
Match: AT5G52800.3 (DNA primases )

HSP 1 Score: 54.7 bits (130), Expect = 9.0e-08
Identity = 23/38 (60.53%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 72  EEDMFMASLVCNVDAECEKLLVCETELHCVKTLQFEME 110
           E D+FM SL+CNV+++CEKLLVC+ E  C+KTL F+ E
Sbjct: 358 ERDVFMTSLICNVESDCEKLLVCKMESDCMKTLCFDTE 395

The following BLAST results are available for this feature:

BLAST of CmoCh18G013040 vs.


Match Name	E-value	Identity	Description

BLAST of CmoCh18G013040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4DSS4	7.9e-20	44.59	DNA-directed primase/polymerase protein isoform X2 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A5D3DH78	1.1e-16	53.47	DNA-directed primase/polymerase protein isoform X2 OS=Cucumis melo var. makuwa O...	[more]
A0A6J1E4L8	5.9e-15	74.14	DNA-directed primase/polymerase protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A1S3AYI4	7.7e-15	48.54	DNA-directed primase/polymerase protein isoform X1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A1S4DSQ0	7.7e-15	48.54	DNA-directed primase/polymerase protein isoform X3 OS=Cucumis melo OX=3656 GN=LO...	[more]

BLAST of CmoCh18G013040 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52800.1	9.0e-08	60.53	DNA primases	[more]
AT5G52800.2	9.0e-08	60.53	DNA primases	[more]
AT5G52800.3	9.0e-08	60.53	DNA primases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044917	DNA-directed primase/polymerase protein	PANTHER	PTHR31399	DNA-DIRECTED PRIMASE / POLYMERASE PROTEIN	coord: 67..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh18G013040.1	CmoCh18G013040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006269	DNA replication, synthesis of RNA primer
biological_process	GO:0042276	error-prone translesion synthesis
biological_process	GO:0006264	mitochondrial DNA replication
biological_process	GO:0031297	replication fork processing
biological_process	GO:0009411	response to UV
cellular_component	GO:0005759	mitochondrial matrix
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003682	chromatin binding
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003896	DNA primase activity