CmoCh18G012300 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh18G012300
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Gibberellin 20 oxidase 2-like
Location	Cmo_Chr18: 12174231 .. 12175876 (+)
RNA-Seq Expression	CmoCh18G012300
Synteny	CmoCh18G012300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTCTTCCCCATCATACTCCCTTCTCAAATCTCTCTCAAGAGGGAATTTCTTTGAATTGCAAGATGGCAACCGAAACCTTATTATCCGCCAACTCAGACGTGAACCAGGAGACGAAAGGGGAGTACCGTGGGACATTTGCGGGCCCCGACGAGTCAAAGGTGCCGGAGAGTTTCGTTTGGCCAGATGATCTTAAGGGATCGCAGGAGGTGCCGGTGCTGCATGTGCCACATATTGACATAAATAAGTTTATGAGTGGCAAGGAAAGTGATGTTGAAGAGGTGACGAGGCTGGTGGATGAGGCTTGTAGAAAACATGGATTCTTTGTGGTGGTGAACCATGGAGTTGATATGAAATTAATGGATAGTCTTCATGAGTGTATGAATGAGTTTTTTTCAATGCCTATGGATGTGAAGCAAAGGGCTCATAGGAAGTTCGGTGAGAATTTTGGATATGCTTATAGCTTCTTTGTGAGATTCTCCTCCAATCTTCCATGGAAGGAAACTCTTTCCCTTCCCTGTGTTGCTGCTGGTGAAAACTCCTCCTCTGCTTATGACTATCTTATTGAAAAATTAGGCTCATCATTCTCCCATCATGGGTAAGTAAATATCTACTAATAATTGGTTTTGATCTTTTTTTTAAGAAGTGTGAGGTGGGTTGATTGTTAATGTAATTTTTATATACTTAATTGAATTAAAGGTGGGAAATGAGATTTGAATAGGTTTGATGGTGTTTTAGGAAGGTGTATCAAGATTGTGCGAAAGCAGTGAACGATCTGGGCATGAAGATATTGGAGGTTTTGGGGTTGAGCCTTGGCGTTTCAAGAGAATACTTCAGGAATTTCTATGAGGACAATGATTCCATAATGAGGCTTAATTATTACCCACCATGCGCGAAGCCAGAACTTGTGTTGGGAACTGGCCCTCATTGTGATCCCACCTCCATAACAATCCTTCACCAAGACAAGGTAAGTGGCTTTCAAGTGAGCGTCGATGATCAATGGTATTCAATTCCTCCAACCCCAGATTCCTTTGTCATCAATATCGGTGACACTTTCAAGGTATGTGTGACACAAAATTACAATTAGAGTTCTTCATTTTTTTCATCTTCCTTGTTCTAAACACAGCTCTTTTGAAATGGGTTGTTTTATTTTTTGTGTTTTGAAGTCTCTCACAAATGGGATTTACAAGAGCTGCGTACACAGAGCTGTAGTGAATAGCGAGAGTGTAAGAAAAACAATGGCTTTCTTTATATGTCCAAATCATGACAAAGTGGTGAGAGCACCGGAGGAATTGGTGGAGAAGAATCCACCACGAAAGTATCCAGATTACACATGGGGAACGTTGCTTGAAATTACTCAAAAGCATTACCGACCTGATTGCAACACTTTGGAAGCCTTAACAGATTGGATTCAAAAGGGAAAACCGTTGGACAATGGCTCCACTATTTCTGCCCCGTCTGCTTAAACCTACCTTAATTATGTGTCTTTGTAGCTCTACTTCTCTATGTATGTCAATAAGTGGTTCTATGTTTATGAGCTTTCTAGGGAAGAAGACCATCGTGTAGCGTTCTGTCTAATGTCTTTCTTCTGTGTTGCTGGAAGTTGCAGGAAAAAAAAACCCTTTAATATACACATGTTTCCTTTCG

mRNA sequence

GTTTCTTCCCCATCATACTCCCTTCTCAAATCTCTCTCAAGAGGGAATTTCTTTGAATTGCAAGATGGCAACCGAAACCTTATTATCCGCCAACTCAGACGTGAACCAGGAGACGAAAGGGGAGTACCGTGGGACATTTGCGGGCCCCGACGAGTCAAAGGTGCCGGAGAGTTTCGTTTGGCCAGATGATCTTAAGGGATCGCAGGAGGTGCCGGTGCTGCATGTGCCACATATTGACATAAATAAGTTTATGAGTGGCAAGGAAAGTGATGTTGAAGAGGTGACGAGGCTGGTGGATGAGGCTTGTAGAAAACATGGATTCTTTGTGGTGGTGAACCATGGAGTTGATATGAAATTAATGGATAGTCTTCATGAGTGTATGAATGAGTTTTTTTCAATGCCTATGGATGTGAAGCAAAGGGCTCATAGGAAGTTCGGTGAGAATTTTGGATATGCTTATAGCTTCTTTGTGAGATTCTCCTCCAATCTTCCATGGAAGGAAACTCTTTCCCTTCCCTGTGTTGCTGCTGGTGAAAACTCCTCCTCTGCTTATGACTATCTTATTGAAAAATTAGGCTCATCATTCTCCCATCATGGGAAGGTGTATCAAGATTGTGCGAAAGCAGTGAACGATCTGGGCATGAAGATATTGGAGGTTTTGGGGTTGAGCCTTGGCGTTTCAAGAGAATACTTCAGGAATTTCTATGAGGACAATGATTCCATAATGAGGCTTAATTATTACCCACCATGCGCGAAGCCAGAACTTGTGTTGGGAACTGGCCCTCATTGTGATCCCACCTCCATAACAATCCTTCACCAAGACAAGGTAAGTGGCTTTCAAGTGAGCGTCGATGATCAATGGTATTCAATTCCTCCAACCCCAGATTCCTTTGTCATCAATATCGGTGACACTTTCAAGTCTCTCACAAATGGGATTTACAAGAGCTGCGTACACAGAGCTGTAGTGAATAGCGAGAGTGTAAGAAAAACAATGGCTTTCTTTATATGTCCAAATCATGACAAAGTGGTGAGAGCACCGGAGGAATTGGTGGAGAAGAATCCACCACGAAAGTATCCAGATTACACATGGGGAACGTTGCTTGAAATTACTCAAAAGCATTACCGACCTGATTGCAACACTTTGGAAGCCTTAACAGATTGGATTCAAAAGGGAAAACCGTTGGACAATGGCTCCACTATTTCTGCCCCGTCTGCTTAAACCTACCTTAATTATGTGTCTTTGTAGCTCTACTTCTCTATGTATGTCAATAAGTGGTTCTATGTTTATGAGCTTTCTAGGGAAGAAGACCATCGTGTAGCGTTCTGTCTAATGTCTTTCTTCTGTGTTGCTGGAAGTTGCAGGAAAAAAAAACCCTTTAATATACACATGTTTCCTTTCG

Coding sequence (CDS)

ATGGCAACCGAAACCTTATTATCCGCCAACTCAGACGTGAACCAGGAGACGAAAGGGGAGTACCGTGGGACATTTGCGGGCCCCGACGAGTCAAAGGTGCCGGAGAGTTTCGTTTGGCCAGATGATCTTAAGGGATCGCAGGAGGTGCCGGTGCTGCATGTGCCACATATTGACATAAATAAGTTTATGAGTGGCAAGGAAAGTGATGTTGAAGAGGTGACGAGGCTGGTGGATGAGGCTTGTAGAAAACATGGATTCTTTGTGGTGGTGAACCATGGAGTTGATATGAAATTAATGGATAGTCTTCATGAGTGTATGAATGAGTTTTTTTCAATGCCTATGGATGTGAAGCAAAGGGCTCATAGGAAGTTCGGTGAGAATTTTGGATATGCTTATAGCTTCTTTGTGAGATTCTCCTCCAATCTTCCATGGAAGGAAACTCTTTCCCTTCCCTGTGTTGCTGCTGGTGAAAACTCCTCCTCTGCTTATGACTATCTTATTGAAAAATTAGGCTCATCATTCTCCCATCATGGGAAGGTGTATCAAGATTGTGCGAAAGCAGTGAACGATCTGGGCATGAAGATATTGGAGGTTTTGGGGTTGAGCCTTGGCGTTTCAAGAGAATACTTCAGGAATTTCTATGAGGACAATGATTCCATAATGAGGCTTAATTATTACCCACCATGCGCGAAGCCAGAACTTGTGTTGGGAACTGGCCCTCATTGTGATCCCACCTCCATAACAATCCTTCACCAAGACAAGGTAAGTGGCTTTCAAGTGAGCGTCGATGATCAATGGTATTCAATTCCTCCAACCCCAGATTCCTTTGTCATCAATATCGGTGACACTTTCAAGTCTCTCACAAATGGGATTTACAAGAGCTGCGTACACAGAGCTGTAGTGAATAGCGAGAGTGTAAGAAAAACAATGGCTTTCTTTATATGTCCAAATCATGACAAAGTGGTGAGAGCACCGGAGGAATTGGTGGAGAAGAATCCACCACGAAAGTATCCAGATTACACATGGGGAACGTTGCTTGAAATTACTCAAAAGCATTACCGACCTGATTGCAACACTTTGGAAGCCTTAACAGATTGGATTCAAAAGGGAAAACCGTTGGACAATGGCTCCACTATTTCTGCCCCGTCTGCTTAA

Protein sequence

MATETLLSANSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPHIDINKFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGENSSSAYDYLIEKLGSSFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSESVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWIQKGKPLDNGSTISAPSA

Homology

[Close]

BLAST of CmoCh18G012300 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 417.9 bits (1073), Expect = 1.2e-115
Identity = 193/340 (56.76%), Postives = 244/340 (71.76%), Query Frame = 0


Query: 30  ESKVPESFVWPDDLKGSQEVPVLHVPHIDINKFMSGKESDVEEVTRLVDEACRKHGFFVV 89
           ++ +P  F+WPDD K S  V  L VP ID+   +S   S + + +RL+ EAC+KHGFF+V
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLSDPSSTL-DASRLISEACKKHGFFLV 96

Query: 90  VNHGVDMKLMDSLHECMNEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHG+  +L+   HE  + FF MP+  KQR  RK GE+ GYA SF  RFS+ LPWKETLS
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 150 LPCVAAGENSSSAYDYLIEKLGSSFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 209
                    S S  DY  + LG  F   GKVYQ+  +A++ L +KI+E+LGLSLGV R+Y
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 210 FRNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSI 269
           FR F+E+NDSIMRLNYYPPC KP+L LGTGPHCDPTS+TILHQD V+G QV V++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 270 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSESVRKTMAFFICPNHDKVVRAPEELV 329
            P P +FV+NIGDTF +L+N  YKSC+HRAVVNSES RK++AFF+CP  D+VV  P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 330 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWIQK 370
           +    R+YPD+TW   LE TQKHYR D NTL+A +DW+ K
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWLTK 375

[Close]

BLAST of CmoCh18G012300 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.2e-115
Identity = 190/338 (56.21%), Postives = 245/338 (72.49%), Query Frame = 0


Query: 30  ESKVPESFVWPDDLKGSQEVPVLHVPHIDINKFMSGKESDVEEVTRLVDEACRKHGFFVV 89
           +S++P  F+WPD+ K S ++P L+VP ID++      +    E  R++ EAC KHGFF+V
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFIDLS-----SQDSTLEAPRVIAEACTKHGFFLV 98

Query: 90  VNHGVDMKLMDSLHECMNEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHGV   L+   H  M  FF MP+  KQ+A RK GE+ GYA SF  RFS+ LPWKETLS
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 150 LPCVAAGENSSSAYDYLIEKLGSSFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 209
                    S +  DY  + LG  F   GKVYQD  +A++ L +KI+E+LGLSLGV+R+Y
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 210 FRNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSI 269
           FR F+E+NDSIMRLN+YPPC  P+L LGTGPHCDP+S+TILHQD V+G QV VD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 270 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSESVRKTMAFFICPNHDKVVRAPEELV 329
            P P +FV+NIGDTF +L+NGI+KSC+HRAVVN ES RK+MAFF+CP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 330 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWI 368
           EK   RKYPD+TW   LE TQKHYR D NTL++ ++W+
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

[Close]

BLAST of CmoCh18G012300 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.3e-112
Identity = 198/375 (52.80%), Postives = 254/375 (67.73%), Query Frame = 0


Query: 1   MATETLLSA----NSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPH 60
           MATE + +     + +  +E    +           +P+ FVWPD  K S +V  L VP 
Sbjct: 1   MATECIATVPQIFSENKTKEDSSIFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPL 60

Query: 61  IDINKFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDV 120
           ID+  F+SG      E TRLV +A  KHGFF++ NHGVD  L+   +  M+ FF  P   
Sbjct: 61  IDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACE 120

Query: 121 KQRAHRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGE-NSSSAYDYLIEKLGSSFS 180
           KQ+A RK+GE+ GYA SF  RFSS LPWKETLS       + +S +  D++ +K+G  + 
Sbjct: 121 KQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYE 180

Query: 181 HHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELV 240
             GKVYQ+ A+A+N L +KI+E+LG+SLGV R YF+ F+ED+DSI RLNYYP C +PEL 
Sbjct: 181 DFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELA 240

Query: 241 LGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSC 300
           LGTGPHCDPTS+TILHQD+V G QV VD++W SIPP P +FV+NIGDTF +LTNG YKSC
Sbjct: 241 LGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSC 300

Query: 301 VHRAVVNSESVRKTMAFFICPNHDKVVRAPEELVE--KNPPRKYPDYTWGTLLEITQKHY 360
           +HRAVVNSE  RKT AFF+CP  +KVV+ PEELV   K+  RKYPD+TW   LE TQKHY
Sbjct: 301 LHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHY 360

Query: 361 RPDCNTLEALTDWIQ 369
           R D NTL+  + W++
Sbjct: 361 RADMNTLDEFSIWLK 375

[Close]

BLAST of CmoCh18G012300 vs. ExPASy Swiss-Prot
Match: Q9C955 (Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GA20OX4 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.4e-111
Identity = 188/339 (55.46%), Postives = 239/339 (70.50%), Query Frame = 0


Query: 33  VPESFVWPDDLKGSQEVPVLHVPHIDINKFMSGKESDVEEVTRLVDEACRKHGFFVVVNH 92
           +P+ FVWPD  K S+ VP+L VP ID+  F+S     V E  RLV EA +KHGFF+V NH
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 93  GVDMKLMDSLHECMNEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLSL-- 152
           GVD +L+ + H+ M+ FF  P   K +A RK GE  GYA SF  RF  NLPWKETLS   
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 153 -PCVAAGENSSSAYDYLIEKLGSSFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 212
            P   +   S +  +Y+ + +G  +   G VYQ+ A+ +++L +KI+E+LG+SLG+ RE+
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 213 FRNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSI 272
           FR F+EDN+SI RLNYYP C +P+LVLGTGPHCDPTS+TIL QD+VSG QV VD+QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 273 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSESVRKTMAFFICPNHDKVVRAPEELV 332
           PP P + V+NIGDT  +LTNGIYKSC+HRAVVN E+ RKT+AFF+CP  DKVV+ P EL 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335

Query: 333 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWIQ 369
                R YPD+TW   LE T KHYR D NTLE  T+W++
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLK 371

[Close]

BLAST of CmoCh18G012300 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.8e-104
Identity = 180/338 (53.25%), Postives = 231/338 (68.34%), Query Frame = 0


Query: 31  SKVPESFVWPDDLKGSQE-VPVLHVPHIDINKFMSGKESDVEEVTRLVDEACRKHGFFVV 90
           S +P  F+WP+    + +    LHVP I+I   +SG  +   EVTRLV EAC +HGFF V
Sbjct: 15  SDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACERHGFFQV 74

Query: 91  VNHGVDMKLMDSLHECMNEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 150
           VNHG+D +L+   H C++ FF+MP+  KQRA R+ GE+ GYA SF  RF+S LPWKETLS
Sbjct: 75  VNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLS 134

Query: 151 LPCVAAGENSSSAYDYLIEKLGSSFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 210
               +   + +   DY++  LG      G+VY      ++ L ++I+EVLG SLGV R +
Sbjct: 135 FR--SCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAH 194

Query: 211 FRNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSI 270
           +R F+E NDSIMRLNYYPPC +P   LGTGPHCDPTS+TILHQD V G QV  + +W SI
Sbjct: 195 YRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSI 254

Query: 271 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSESVRKTMAFFICPNHDKVVRAPEELV 330
            P  D+FV+NIGDTF +L+NG YKSC+HRAVVNS   RK++AFF+CP  DKVV  P  LV
Sbjct: 255 RPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLV 314

Query: 331 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWI 368
           + + PR YPD+TW +LL+ TQKHYR D  TLE  + WI
Sbjct: 315 DASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWI 350

[Close]

BLAST of CmoCh18G012300 vs. ExPASy TrEMBL
Match: A0A6J1G1X5 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449915 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.0e-229
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0


Query: 1   MATETLLSANSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPHIDIN 60
           MATETLLSANSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPHIDIN
Sbjct: 73  MATETLLSANSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPHIDIN 132

Query: 61  KFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDVKQRA 120
           KFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDVKQRA
Sbjct: 133 KFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDVKQRA 192

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGENSSSAYDYLIEKLGSSFSHHGKV 180
           HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGENSSSAYDYLIEKLGSSFSHHGKV
Sbjct: 193 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGENSSSAYDYLIEKLGSSFSHHGKV 252

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELVLGTGP
Sbjct: 253 YQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELVLGTGP 312

Query: 241 HCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           HCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV
Sbjct: 313 HCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 372

Query: 301 VNSESVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VNSESVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL
Sbjct: 373 VNSESVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 432

Query: 361 EALTDWIQKGKPLDNGSTISAPSA 385
           EALTDWIQKGKPLDNGSTISAPSA
Sbjct: 433 EALTDWIQKGKPLDNGSTISAPSA 456

[Close]

BLAST of CmoCh18G012300 vs. ExPASy TrEMBL
Match: A0A6J1HWU5 (gibberellin 20 oxidase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467571 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 9.4e-196
Identity = 320/384 (83.33%), Postives = 355/384 (92.45%), Query Frame = 0


Query: 1   MATETLLSANSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPHIDIN 60
           MATE+L SA+SD+NQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVL+VPHID+N
Sbjct: 1   MATESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLN 60

Query: 61  KFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDVKQRA 120
           KF+SG ESDVEEVTRLVDEACRKHGFFVVVNHGVDMK+M+SLHECM+EFFSMPMDVKQRA
Sbjct: 61  KFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRA 120

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGENSSSAYDYLIEKLGSSFSHHGKV 180
           HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVA GENSSSAYDYLIEKLG +FSHHGK 
Sbjct: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKA 180

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C  A+N+LG KI+E+LGLSLG+SREYF+NFY+DNDSI+RLNYYP C KPE+VLGTGP
Sbjct: 181 YQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGTGP 240

Query: 241 HCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           H DPTS+TILHQD VSG QV  +DQWYSIPP P++FVINIGDTF SLTNGIYK C+HRAV
Sbjct: 241 HTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAV 300

Query: 301 VNSESVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VNS + RK++AFF+CP+HDKVVRAPEELVEK+PPRKYPDY W  LLE+TQK YRPDCNTL
Sbjct: 301 VNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTL 360

Query: 361 EALTDWIQKGKPLDNGSTISAPSA 385
           EA   W+Q+GK LD GSTI+APSA
Sbjct: 361 EAFKTWVQEGKALDTGSTITAPSA 384

[Close]

BLAST of CmoCh18G012300 vs. ExPASy TrEMBL
Match: A0A6J1G185 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449755 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 2.2e-176
Identity = 297/384 (77.34%), Postives = 334/384 (86.98%), Query Frame = 0


Query: 1   MATETLLSANSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPHIDIN 60
           MAT++ LSA+ ++NQE K E R TF G D S VPE F+WPD+ KGSQ +P LHVPHID+N
Sbjct: 7   MATKSSLSAHLNLNQEMKRERRETFGGSDGSMVPEYFIWPDEFKGSQLLPELHVPHIDLN 66

Query: 61  KFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDVKQRA 120
           KF+SG ESDVEE TRLVDEACR+HGFFVVVNHGVDM+LM  +HEC++EFF+MP+DVKQRA
Sbjct: 67  KFLSGDESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQRA 126

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGENSSSAYDYLIEKLGSSFSHHGKV 180
            RK GEN+GY  SFF RF+S LPWKET SL CVAA +NSS+A DY+I+ LGS+FSHHGKV
Sbjct: 127 QRKVGENYGYTNSFFGRFASKLPWKETFSLRCVAA-QNSSTARDYVIDTLGSTFSHHGKV 186

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C KA+NDLGMKILE+LGLSLGVSREYF+NFYEDNDSI+RLNYYPPC KPE+VLGTGP
Sbjct: 187 YQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPPCEKPEVVLGTGP 246

Query: 241 HCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           H DPTS+TILHQD VSG QV V+DQWYSIPPTPDSFV+NIGDTF SLTNGIYKSCVHRAV
Sbjct: 247 HTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSCVHRAV 306

Query: 301 VNSESVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VN +  RK+MAFFICP HDKVVRAPEELVEKNPPRKYPDYTW  LLE+TQKHYR DCNTL
Sbjct: 307 VNCKEARKSMAFFICPAHDKVVRAPEELVEKNPPRKYPDYTWPMLLEMTQKHYRADCNTL 366

Query: 361 EALTDWIQKGKPLDNGSTISAPSA 385
           EA T WIQK K LD G TI+ PSA
Sbjct: 367 EAFTTWIQKEKLLDTGCTIT-PSA 388

[Close]

BLAST of CmoCh18G012300 vs. ExPASy TrEMBL
Match: A0A6J1G180 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449753 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.5e-164
Identity = 273/384 (71.09%), Postives = 324/384 (84.38%), Query Frame = 0


Query: 1   MATETLLSANSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPHIDIN 60
           +ATE   SA S +N+ETKGEYR  F G DESKVPE F+W +  + S+ +PVL VP ID+ 
Sbjct: 7   VATE---SAPSSLNEETKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLE 66

Query: 61  KFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDVKQRA 120
           KFMSG +S VEE TRLVDEACR+HG F VVNHGVDM++M  +H+CMNEFF+MP+DVKQRA
Sbjct: 67  KFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDMEMMGRVHDCMNEFFTMPLDVKQRA 126

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGENSSSAYDYLIEKLGSSFSHHGKV 180
            R+ GE++GY  SFF RF++NLPWKET SL CVAA +NSS+A+DY+++ LGSSFSHHGK 
Sbjct: 127 KRRVGESYGYTNSFFGRFANNLPWKETFSLRCVAA-QNSSAAHDYVLDTLGSSFSHHGKA 186

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C  A+N LGMKI+E+LGLSLGVSREYF+NFYEDNDSI+RLNYYP C KPE+VLGTGP
Sbjct: 187 YQECGIALNQLGMKIVELLGLSLGVSREYFKNFYEDNDSILRLNYYPTCDKPEVVLGTGP 246

Query: 241 HCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           H DPTS+T+LHQD VSG QV  +DQWYSIPP P++FVINIGDTF SLTNGIYK C+HRAV
Sbjct: 247 HTDPTSVTLLHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAV 306

Query: 301 VNSESVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VNS + RK++AFF+CP+HDKVVRAPEELVEK+PPRKYPDY W  LLE+TQK YRPDCNTL
Sbjct: 307 VNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTL 366

Query: 361 EALTDWIQKGKPLDNGSTISAPSA 385
           EA   W++ GK LD GSTI+APSA
Sbjct: 367 EAFKTWVKDGKALDTGSTITAPSA 386

[Close]

BLAST of CmoCh18G012300 vs. ExPASy TrEMBL
Match: A0A6J1HZ92 (gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467571 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.3e-164
Identity = 272/384 (70.83%), Postives = 325/384 (84.64%), Query Frame = 0


Query: 1   MATETLLSANSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPHIDIN 60
           +ATE   SA S++N+E KGEYR  F G DESKVPE F+W +  + S+ +PVL VP ID+ 
Sbjct: 7   VATE---SAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLE 66

Query: 61  KFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDVKQRA 120
           KFMSG +S VEE TRLVDEACR+HG F VVNHGVD+++M  +H+CMNEFF+MP+DVKQRA
Sbjct: 67  KFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRA 126

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGENSSSAYDYLIEKLGSSFSHHGKV 180
            RK GE++GY  SFF RF+SNLPWKET SL CVAA +NSS+A+DY+++ LGSSFSHHGK 
Sbjct: 127 KRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAA-QNSSAAHDYVLDTLGSSFSHHGKA 186

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C  A+N+LG KI+E+LGLSLG+SREYF+NFY+DNDSI+RLNYYP C KPE+VLGTGP
Sbjct: 187 YQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGTGP 246

Query: 241 HCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           H DPTS+TILHQD VSG QV  +DQWYSIPP P++FVINIGDTF SLTNGIYK C+HRAV
Sbjct: 247 HTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAV 306

Query: 301 VNSESVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VNS + RK++AFF+CP+HDKVVRAPEELVEK+PPRKYPDY W  LLE+TQK YRPDCNTL
Sbjct: 307 VNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTL 366

Query: 361 EALTDWIQKGKPLDNGSTISAPSA 385
           EA   W+Q+GK LD GSTI+APSA
Sbjct: 367 EAFKTWVQEGKALDTGSTITAPSA 386

[Close]

BLAST of CmoCh18G012300 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 417.9 bits (1073), Expect = 8.6e-117
Identity = 193/340 (56.76%), Postives = 244/340 (71.76%), Query Frame = 0


Query: 30  ESKVPESFVWPDDLKGSQEVPVLHVPHIDINKFMSGKESDVEEVTRLVDEACRKHGFFVV 89
           ++ +P  F+WPDD K S  V  L VP ID+   +S   S + + +RL+ EAC+KHGFF+V
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLSDPSSTL-DASRLISEACKKHGFFLV 96

Query: 90  VNHGVDMKLMDSLHECMNEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHG+  +L+   HE  + FF MP+  KQR  RK GE+ GYA SF  RFS+ LPWKETLS
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 150 LPCVAAGENSSSAYDYLIEKLGSSFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 209
                    S S  DY  + LG  F   GKVYQ+  +A++ L +KI+E+LGLSLGV R+Y
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 210 FRNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSI 269
           FR F+E+NDSIMRLNYYPPC KP+L LGTGPHCDPTS+TILHQD V+G QV V++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 270 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSESVRKTMAFFICPNHDKVVRAPEELV 329
            P P +FV+NIGDTF +L+N  YKSC+HRAVVNSES RK++AFF+CP  D+VV  P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 330 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWIQK 370
           +    R+YPD+TW   LE TQKHYR D NTL+A +DW+ K
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWLTK 375

[Close]

BLAST of CmoCh18G012300 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 417.9 bits (1073), Expect = 8.6e-117
Identity = 190/338 (56.21%), Postives = 245/338 (72.49%), Query Frame = 0


Query: 30  ESKVPESFVWPDDLKGSQEVPVLHVPHIDINKFMSGKESDVEEVTRLVDEACRKHGFFVV 89
           +S++P  F+WPD+ K S ++P L+VP ID++      +    E  R++ EAC KHGFF+V
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFIDLS-----SQDSTLEAPRVIAEACTKHGFFLV 98

Query: 90  VNHGVDMKLMDSLHECMNEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHGV   L+   H  M  FF MP+  KQ+A RK GE+ GYA SF  RFS+ LPWKETLS
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 150 LPCVAAGENSSSAYDYLIEKLGSSFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 209
                    S +  DY  + LG  F   GKVYQD  +A++ L +KI+E+LGLSLGV+R+Y
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 210 FRNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSI 269
           FR F+E+NDSIMRLN+YPPC  P+L LGTGPHCDP+S+TILHQD V+G QV VD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 270 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSESVRKTMAFFICPNHDKVVRAPEELV 329
            P P +FV+NIGDTF +L+NGI+KSC+HRAVVN ES RK+MAFF+CP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 330 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWI 368
           EK   RKYPD+TW   LE TQKHYR D NTL++ ++W+
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

[Close]

BLAST of CmoCh18G012300 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 407.9 bits (1047), Expect = 8.9e-114
Identity = 198/375 (52.80%), Postives = 254/375 (67.73%), Query Frame = 0


Query: 1   MATETLLSA----NSDVNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLHVPH 60
           MATE + +     + +  +E    +           +P+ FVWPD  K S +V  L VP 
Sbjct: 1   MATECIATVPQIFSENKTKEDSSIFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPL 60

Query: 61  IDINKFMSGKESDVEEVTRLVDEACRKHGFFVVVNHGVDMKLMDSLHECMNEFFSMPMDV 120
           ID+  F+SG      E TRLV +A  KHGFF++ NHGVD  L+   +  M+ FF  P   
Sbjct: 61  IDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACE 120

Query: 121 KQRAHRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVAAGE-NSSSAYDYLIEKLGSSFS 180
           KQ+A RK+GE+ GYA SF  RFSS LPWKETLS       + +S +  D++ +K+G  + 
Sbjct: 121 KQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYE 180

Query: 181 HHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREYFRNFYEDNDSIMRLNYYPPCAKPELV 240
             GKVYQ+ A+A+N L +KI+E+LG+SLGV R YF+ F+ED+DSI RLNYYP C +PEL 
Sbjct: 181 DFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELA 240

Query: 241 LGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSC 300
           LGTGPHCDPTS+TILHQD+V G QV VD++W SIPP P +FV+NIGDTF +LTNG YKSC
Sbjct: 241 LGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSC 300

Query: 301 VHRAVVNSESVRKTMAFFICPNHDKVVRAPEELVE--KNPPRKYPDYTWGTLLEITQKHY 360
           +HRAVVNSE  RKT AFF+CP  +KVV+ PEELV   K+  RKYPD+TW   LE TQKHY
Sbjct: 301 LHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHY 360

Query: 361 RPDCNTLEALTDWIQ 369
           R D NTL+  + W++
Sbjct: 361 RADMNTLDEFSIWLK 375

[Close]

BLAST of CmoCh18G012300 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 403.7 bits (1036), Expect = 1.7e-112
Identity = 188/339 (55.46%), Postives = 239/339 (70.50%), Query Frame = 0


Query: 33  VPESFVWPDDLKGSQEVPVLHVPHIDINKFMSGKESDVEEVTRLVDEACRKHGFFVVVNH 92
           +P+ FVWPD  K S+ VP+L VP ID+  F+S     V E  RLV EA +KHGFF+V NH
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 93  GVDMKLMDSLHECMNEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLSL-- 152
           GVD +L+ + H+ M+ FF  P   K +A RK GE  GYA SF  RF  NLPWKETLS   
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 153 -PCVAAGENSSSAYDYLIEKLGSSFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 212
            P   +   S +  +Y+ + +G  +   G VYQ+ A+ +++L +KI+E+LG+SLG+ RE+
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 213 FRNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVSVDDQWYSI 272
           FR F+EDN+SI RLNYYP C +P+LVLGTGPHCDPTS+TIL QD+VSG QV VD+QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 273 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSESVRKTMAFFICPNHDKVVRAPEELV 332
           PP P + V+NIGDT  +LTNGIYKSC+HRAVVN E+ RKT+AFF+CP  DKVV+ P EL 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335

Query: 333 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWIQ 369
                R YPD+TW   LE T KHYR D NTLE  T+W++
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLK 371

[Close]

BLAST of CmoCh18G012300 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 296.6 bits (758), Expect = 2.9e-80
Identity = 147/340 (43.24%), Postives = 203/340 (59.71%), Query Frame = 0


Query: 30  ESKVPESFVWPDDLKGSQEVPVLHVPHIDINKFMSGKESDVEEVTRLVDEACRKHGFFVV 89
           +  VP  F WP+      E   L +P ID++ F++G E++ +   + V +AC  HG F+V
Sbjct: 40  QPNVPAEFFWPEKDVAPSEGD-LDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLV 99

Query: 90  VNHGVDMKLMDSLHECMNEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHG    L +   E  + FF +  D K RA+R  G   GY      RFSSNLPW ETL+
Sbjct: 100 VNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLT 159

Query: 150 LPCVAAGENSSSAYDYLIEKLGSSFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGV-SRE 209
           L       +     D+L  +LG+     G+V+Q+   A+N L M ++E+LG+S+G+  R 
Sbjct: 160 LAFKKGPPH--VVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDRT 219

Query: 210 YFRNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVSVDDQWYS 269
           Y+R F+ED   I R NYYPPC +PE  LG GPH DPT+IT+L QD V G +V     W +
Sbjct: 220 YYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQT 279

Query: 270 IPPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSESVRKTMAFFICPNHDKVVRAPEEL 329
           + P P + V+N+GDTF +L+NG Y+SC HRAVVN E VR+++ FF CP  DK++  P EL
Sbjct: 280 VRPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPEL 339

Query: 330 VE-KNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWI 368
           VE +   RKYPD+TW  L + TQ  YR D  TL   + W+
Sbjct: 340 VEGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWL 376

The following BLAST results are available for this feature:

BLAST of CmoCh18G012300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39110	1.2e-115	56.76	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]
Q39111	1.2e-115	56.21	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1	[more]
Q39112	1.3e-112	52.80	Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1	[more]
Q9C955	2.4e-111	55.46	Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GA20OX4 PE=2 SV=1	[more]
O04707	2.8e-104	53.25	Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1	[more]

BLAST of CmoCh18G012300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1X5	1.0e-229	100.00	gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449915 PE=3...	[more]
A0A6J1HWU5	9.4e-196	83.33	gibberellin 20 oxidase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1G185	2.2e-176	77.34	gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449755 PE=3...	[more]
A0A6J1G180	2.5e-164	71.09	gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449753 PE=3...	[more]
A0A6J1HZ92	7.3e-164	70.83	gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]

BLAST of CmoCh18G012300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25420.1	8.6e-117	56.76	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51810.1	8.6e-117	56.21	gibberellin 20 oxidase 2	[more]
AT5G07200.1	8.9e-114	52.80	gibberellin 20-oxidase 3	[more]
AT1G60980.1	1.7e-112	55.46	gibberellin 20-oxidase 4	[more]
AT1G44090.1	2.9e-80	43.24	gibberellin 20-oxidase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 241..262 score: 40.08 coord: 276..302 score: 30.98 coord: 336..354 score: 24.4 coord: 77..94 score: 56.06
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..18
None	No IPR available	PANTHER	PTHR47990:SF26	GIBBERELLIN 20-OXIDASE	coord: 25..368
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 25..368
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 29..355
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 25..353 e-value: 3.3E-88 score: 298.2
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 218..316 e-value: 1.4E-26 score: 93.0
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 54..155 e-value: 4.0E-22 score: 79.2
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 216..316 score: 13.639443

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh18G012300.1	CmoCh18G012300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity