CmoCh18G012280 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh18G012280
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	gibberellin 20 oxidase 2-like
Location	Cmo_Chr18: 12164173 .. 12165710 (+)
RNA-Seq Expression	CmoCh18G012280
Synteny	CmoCh18G012280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCTGAATTGCATGAACGCAACGGAATCCTCACCGGCGTTGAACCAAGTGAGGAATAGGGAGTACCGACCGGCATTTTGTGGCTCCGACGAGTCAAAGATACCGGAGGATTTCATTTGGCCAGAAGAGTTTAAGGCGGCGGAGGTGGTGCCGGAGCTGCATGTTCCACATATTGACTTGAAGAAGTTTTTTAGTGGCAATGAAAGTGACGTTGAAGAGGCGACGAGGCTGGTGGATGAGGCTTGTAGAAAACATGGATTCTTTGTGGTGGTGAACCATGGAGTTGATATGGAATTGATGAAAGGTGTTCATGAGTGTATCGATGAGTTCTTTACAATGCCTTTGGATGTGAAGCAGAGGGCTCAAAGGAAGGTAGGTGAGAATTATGGATATACTAATAGTTTCATTGGGAGATTCGCGTCCAAGCTTCCTTGGAAGGAAACCTTTTCCCTTCGCTGTGTGGCTGCTCAAAACTCCTCCGCTGCTCATGACTATGTTCTTGACACTTTAGGCTCGTCATTCTCCCATCATGGGTATCTAAATTCCACTATCTCTACCTATGCCTATTTAATCCTTACACTTTTTTTAATTGTTTGATGGTGTTTTAGGAAGTTGTATCAAGAGTGTGGGATAGCATTGAACGAGCTTGGTATGAAGATTGTGGAGCTTTTGGGGCTGAGCCTTGGCATTTCAAGAGAATGCTTCAAGAATTTCTATGAGGACAATGATTCAATATTGAGGCTTAATTATTACCCGATATGCGAGAAGCCAGAATTGGTGTTGGGAACTGGCCCTCATAGTGATCCTACCTCCATCACAATCCTTCACCAAGACCATGTTAGTGGCCTTCAAGTGTTCGTAGATGATCAATGGTATTCAATTCCTCCAACTCCGGATTCCTTTGTCATCAACATTGGTGACACTTTCATGGTATGTATATGCAACACAAAATTAGAATTAGGGTTTCTTCAAGATCTCTTGCGATATTGTCCACTTAGAATATAAGCTCTCATGACTTTGCTTTAAGCTTCCCAAAAAGACCTTACGTCAATTGAGATATATTCCAATTTCTTATTCATAGACCCATGATCTTTTTCTAGATCTTTCTCTAAATTAGCTAACTAGCTAACGTGGGACTTCCTGCCAATAATTCTCGGCATTAACATTAGGGTTTTCATTTTTCATATGAACTTGACTAGTTTTGAAATGGGTTGTTTTATTTTTTGTGTTTGAAGTCTCTGACAAATGGGATTTACAAGAGCTGCTTGCACCGAGCAGTAGTGAACTGCAAGGAATCAAGAAAATCATTGGCGTTCTTTCTATGTCCAGCGCATGACAAAGTGGTGAGAGCACCGGAGGAGTTGGTTGAGAAGAATCCACCAAGGAATTATTCAGATTACACATGGGAGATGTTGCTTAAAATGACCCAAAAGGATTACAGGGCTGATTTCAAGACTTTGGAAGCCTTCATGGCTCGTGTTCAAAAGGAAAAGTCGTTGGAGGACACTACCTCCCCAGCTGCGACGTCTGCTTAA

mRNA sequence

ATGGCGCTGAATTGCATGAACGCAACGGAATCCTCACCGGCGTTGAACCAAGTGAGGAATAGGGAGTACCGACCGGCATTTTGTGGCTCCGACGAGTCAAAGATACCGGAGGATTTCATTTGGCCAGAAGAGTTTAAGGCGGCGGAGGTGGTGCCGGAGCTGCATGTTCCACATATTGACTTGAAGAAGTTTTTTAGTGGCAATGAAAGTGACGTTGAAGAGGCGACGAGGCTGGTGGATGAGGCTTGTAGAAAACATGGATTCTTTGTGGTGGTGAACCATGGAGTTGATATGGAATTGATGAAAGGTGTTCATGAGTGTATCGATGAGTTCTTTACAATGCCTTTGGATGTGAAGCAGAGGGCTCAAAGGAAGGTAGGTGAGAATTATGGATATACTAATAGTTTCATTGGGAGATTCGCGTCCAAGCTTCCTTGGAAGGAAACCTTTTCCCTTCGCTGTGTGGCTGCTCAAAACTCCTCCGCTGCTCATGACTATGTTCTTGACACTTTAGGCTCGTCATTCTCCCATCATGGGAAGTTGTATCAAGAGTGTGGGATAGCATTGAACGAGCTTGGTATGAAGATTGTGGAGCTTTTGGGGCTGAGCCTTGGCATTTCAAGAGAATGCTTCAAGAATTTCTATGAGGACAATGATTCAATATTGAGGCTTAATTATTACCCGATATGCGAGAAGCCAGAATTGGTGTTGGGAACTGGCCCTCATAGTGATCCTACCTCCATCACAATCCTTCACCAAGACCATGTTAGTGGCCTTCAAGTGTTCGTAGATGATCAATGGTATTCAATTCCTCCAACTCCGGATTCCTTTGTCATCAACATTGGTGACACTTTCATGTCTCTGACAAATGGGATTTACAAGAGCTGCTTGCACCGAGCAGTAGTGAACTGCAAGGAATCAAGAAAATCATTGGCGTTCTTTCTATGTCCAGCGCATGACAAAGTGGTGAGAGCACCGGAGGAGTTGGTTGAGAAGAATCCACCAAGGAATTATTCAGATTACACATGGGAGATGTTGCTTAAAATGACCCAAAAGGATTACAGGGCTGATTTCAAGACTTTGGAAGCCTTCATGGCTCGTGTTCAAAAGGAAAAGTCGTTGGAGGACACTACCTCCCCAGCTGCGACGTCTGCTTAA

Coding sequence (CDS)

ATGGCGCTGAATTGCATGAACGCAACGGAATCCTCACCGGCGTTGAACCAAGTGAGGAATAGGGAGTACCGACCGGCATTTTGTGGCTCCGACGAGTCAAAGATACCGGAGGATTTCATTTGGCCAGAAGAGTTTAAGGCGGCGGAGGTGGTGCCGGAGCTGCATGTTCCACATATTGACTTGAAGAAGTTTTTTAGTGGCAATGAAAGTGACGTTGAAGAGGCGACGAGGCTGGTGGATGAGGCTTGTAGAAAACATGGATTCTTTGTGGTGGTGAACCATGGAGTTGATATGGAATTGATGAAAGGTGTTCATGAGTGTATCGATGAGTTCTTTACAATGCCTTTGGATGTGAAGCAGAGGGCTCAAAGGAAGGTAGGTGAGAATTATGGATATACTAATAGTTTCATTGGGAGATTCGCGTCCAAGCTTCCTTGGAAGGAAACCTTTTCCCTTCGCTGTGTGGCTGCTCAAAACTCCTCCGCTGCTCATGACTATGTTCTTGACACTTTAGGCTCGTCATTCTCCCATCATGGGAAGTTGTATCAAGAGTGTGGGATAGCATTGAACGAGCTTGGTATGAAGATTGTGGAGCTTTTGGGGCTGAGCCTTGGCATTTCAAGAGAATGCTTCAAGAATTTCTATGAGGACAATGATTCAATATTGAGGCTTAATTATTACCCGATATGCGAGAAGCCAGAATTGGTGTTGGGAACTGGCCCTCATAGTGATCCTACCTCCATCACAATCCTTCACCAAGACCATGTTAGTGGCCTTCAAGTGTTCGTAGATGATCAATGGTATTCAATTCCTCCAACTCCGGATTCCTTTGTCATCAACATTGGTGACACTTTCATGTCTCTGACAAATGGGATTTACAAGAGCTGCTTGCACCGAGCAGTAGTGAACTGCAAGGAATCAAGAAAATCATTGGCGTTCTTTCTATGTCCAGCGCATGACAAAGTGGTGAGAGCACCGGAGGAGTTGGTTGAGAAGAATCCACCAAGGAATTATTCAGATTACACATGGGAGATGTTGCTTAAAATGACCCAAAAGGATTACAGGGCTGATTTCAAGACTTTGGAAGCCTTCATGGCTCGTGTTCAAAAGGAAAAGTCGTTGGAGGACACTACCTCCCCAGCTGCGACGTCTGCTTAA

Protein sequence

MALNCMNATESSPALNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAFMARVQKEKSLEDTTSPAATSA

Homology

[Close]

BLAST of CmoCh18G012280 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 406.0 bits (1042), Expect = 4.8e-112
Identity = 194/334 (58.08%), Postives = 240/334 (71.86%), Query Frame = 0


Query: 32  ESKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVV 91
           ++ IP  FIWP++ K +  V EL VP IDL+   S + S   +A+RL+ EAC+KHGFF+V
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96

Query: 92  VNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFS 151
           VNHG+  EL+   HE    FF MPL  KQR  RK GE+ GY +SF GRF++KLPWKET S
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 152 LR-CVAAQNSSAAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 211
            R C     S +  DY  D LG  F   GK+YQE   A++ L +KI+ELLGLSLG+ R+ 
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 212 FKNFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 271
           F+ F+E+NDSI+RLNYYP C KP+L LGTGPH DPTS+TILHQDHV+GLQVFV++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 272 PPTPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 331
            P P +FV+NIGDTFM+L+N  YKSCLHRAVVN +  RKSLAFFLCP  D+VV  P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 332 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 365
           +    R Y D+TW M L+ TQK YRAD  TL+AF
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAF 369

[Close]

BLAST of CmoCh18G012280 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 4.8e-112
Identity = 190/334 (56.89%), Postives = 247/334 (73.95%), Query Frame = 0


Query: 32  ESKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVV 91
           +S+IP  FIWP+E K +  +PEL+VP IDL      ++    EA R++ EAC KHGFF+V
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLV 98

Query: 92  VNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFS 151
           VNHGV   L+   H  ++ FF MPL  KQ+AQRK GE+ GY +SF GRF++KLPWKET S
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 152 LRCVAAQNSS-AAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 211
            +     + S    DY  DTLG  F   GK+YQ+   A++ L +KI+ELLGLSLG++R+ 
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 212 FKNFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 271
           F+ F+E+NDSI+RLN+YP C+ P+L LGTGPH DP+S+TILHQDHV+GLQVFVD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 272 PPTPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 331
            P P +FV+NIGDTFM+L+NGI+KSCLHRAVVN + +RKS+AFFLCP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 332 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 365
           EK   R Y D+TW M L+ TQK YRAD  TL++F
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSF 367

[Close]

BLAST of CmoCh18G012280 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.2e-109
Identity = 191/343 (55.69%), Postives = 240/343 (69.97%), Query Frame = 0


Query: 35  IPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNH 94
           IP+ F+WP+  K +  V  L VP IDL  F SG+     EATRLV +A  KHGFF++ NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 95  GVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRC 154
           GVD  L+   +  +D FF  P   KQ+AQRK GE+ GY +SF+GRF+SKLPWKET S + 
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156

Query: 155 VAAQ--NSSAAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGISRECFK 214
              +  +S    D+V   +G  +   GK+YQE   A+N L +KI+ELLG+SLG+ R  FK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216

Query: 215 NFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSIPP 274
            F+ED+DSI RLNYYP C++PEL LGTGPH DPTS+TILHQD V GLQVFVD++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276

Query: 275 TPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELVE- 334
            P +FV+NIGDTFM+LTNG YKSCLHRAVVN +  RK+ AFFLCP  +KVV+ PEELV  
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336

Query: 335 -KNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAFMARVQKEKS 374
            K+  R Y D+TW M L+ TQK YRAD  TL+ F   ++  +S
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRRS 379

[Close]

BLAST of CmoCh18G012280 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 4.6e-107
Identity = 194/364 (53.30%), Postives = 243/364 (66.76%), Query Frame = 0


Query: 24  RPAFCG---SDESKIPEDFIWPE-EFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLV 83
           RP F     S  S IP  FIWPE E    +   ELHVP I++    SG+ +   E TRLV
Sbjct: 3   RPVFDAAVLSGRSDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLV 62

Query: 84  DEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGR 143
            EAC +HGFF VVNHG+D EL+   H C+D FFTMPL  KQRA R+ GE+ GY +SF GR
Sbjct: 63  GEACERHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGR 122

Query: 144 FASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVEL 203
           FASKLPWKET S R     + +   DY++ TLG      G++Y      ++ L ++I+E+
Sbjct: 123 FASKLPWKETLSFRS-CPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEV 182

Query: 204 LGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGL 263
           LG SLG+ R  ++ F+E NDSI+RLNYYP C++P   LGTGPH DPTS+TILHQD+V GL
Sbjct: 183 LGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGL 242

Query: 264 QVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAH 323
           QV  + +W SI P  D+FV+NIGDTFM+L+NG YKSCLHRAVVN +  RKSLAFFLCP  
Sbjct: 243 QVHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEM 302

Query: 324 DKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAFMARVQKEKSLEDTTS 383
           DKVV  P  LV+ + PR Y D+TW  LL  TQK YRAD KTLE F + + +++  +    
Sbjct: 303 DKVVAPPGTLVDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQQQQGQLALQ 362

[Close]

BLAST of CmoCh18G012280 vs. ExPASy Swiss-Prot
Match: Q9C955 (Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GA20OX4 PE=2 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.8e-106
Identity = 188/334 (56.29%), Postives = 231/334 (69.16%), Query Frame = 0


Query: 35  IPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNH 94
           IP++F+WP+  K ++ VP L VP IDL  F S +   V EA RLV EA +KHGFF+V NH
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 95  GVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRC 154
           GVD  L+   H+ +D FF  P   K +AQRKVGE  GY +SF+GRF   LPWKET S   
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 155 VAAQN----SSAAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 214
              +     S    +Y+  T+G  +   G +YQE    ++ L +KI+ELLG+SLGI RE 
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 215 FKNFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 274
           F+ F+EDN+SI RLNYYP C++P+LVLGTGPH DPTS+TIL QD VSGLQVFVD+QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 275 PPTPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 334
           PP P + V+NIGDT M+LTNGIYKSCLHRAVVN + +RK+LAFFLCP  DKVV+ P EL 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335

Query: 335 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 365
                R Y D+TW M L+ T K YRAD  TLE F
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEF 366

[Close]

BLAST of CmoCh18G012280 vs. ExPASy TrEMBL
Match: A0A6J1G194 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449754 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 1.6e-227
Identity = 385/385 (100.00%), Postives = 385/385 (100.00%), Query Frame = 0


Query: 1   MALNCMNATESSPALNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHID 60
           MALNCMNATESSPALNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHID
Sbjct: 1   MALNCMNATESSPALNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHID 60

Query: 61  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 120
           LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ
Sbjct: 61  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 120

Query: 121 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK 180
           RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK
Sbjct: 121 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK 180

Query: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGTG 240
           LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGTG
Sbjct: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGTG 240

Query: 241 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300
           PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA
Sbjct: 241 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300

Query: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 360
           VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT
Sbjct: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 360

Query: 361 LEAFMARVQKEKSLEDTTSPAATSA 386
           LEAFMARVQKEKSLEDTTSPAATSA
Sbjct: 361 LEAFMARVQKEKSLEDTTSPAATSA 385

[Close]

BLAST of CmoCh18G012280 vs. ExPASy TrEMBL
Match: A0A6J1HUH4 (gibberellin 20 oxidase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467572 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.1e-219
Identity = 369/385 (95.84%), Postives = 377/385 (97.92%), Query Frame = 0


Query: 1   MALNCMNATESSPALNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHID 60
           MALNCMNATESSPALNQV+NREY PAFCGSDESKIPE+FIWPEEFKAAEVVPELHVPHID
Sbjct: 1   MALNCMNATESSPALNQVKNREYLPAFCGSDESKIPEEFIWPEEFKAAEVVPELHVPHID 60

Query: 61  LKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVKQ 120
            KKFFSGNESD+EEATRLVDEACRKHGFFV+VNHGVDMELMK VHEC++EFFTMPLDVKQ
Sbjct: 61  FKKFFSGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELMKAVHECMNEFFTMPLDVKQ 120

Query: 121 RAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK 180
           RA RKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK
Sbjct: 121 RAGRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGK 180

Query: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGTG 240
           LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYP C KPELVLGTG
Sbjct: 181 LYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPTCGKPELVLGTG 240

Query: 241 PHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300
           PHSDPTS+TILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA
Sbjct: 241 PHSDPTSVTILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHRA 300

Query: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFKT 360
           VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNY DYTWEMLL+MTQKDYRADFKT
Sbjct: 301 VVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYPDYTWEMLLEMTQKDYRADFKT 360

Query: 361 LEAFMARVQKEKSLEDTTSPAATSA 386
           LEAFMARVQKEKSLEDT SPAATSA
Sbjct: 361 LEAFMARVQKEKSLEDTASPAATSA 385

[Close]

BLAST of CmoCh18G012280 vs. ExPASy TrEMBL
Match: A0A6J1G185 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449755 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.3e-184
Identity = 314/388 (80.93%), Postives = 345/388 (88.92%), Query Frame = 0


Query: 1   MALNCMNATESSPA----LNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHV 60
           MA NC  AT+SS +    LNQ   RE R  F GSD S +PE FIWP+EFK ++++PELHV
Sbjct: 1   MAFNCKMATKSSLSAHLNLNQEMKRERRETFGGSDGSMVPEYFIWPDEFKGSQLLPELHV 60

Query: 61  PHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPL 120
           PHIDL KF SG+ESDVEEATRLVDEACR+HGFFVVVNHGVDMELMKGVHECIDEFFTMPL
Sbjct: 61  PHIDLNKFLSGDESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECIDEFFTMPL 120

Query: 121 DVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFS 180
           DVKQRAQRKVGENYGYTNSF GRFASKLPWKETFSLRCVAAQNSS A DYV+DTLGS+FS
Sbjct: 121 DVKQRAQRKVGENYGYTNSFFGRFASKLPWKETFSLRCVAAQNSSTARDYVIDTLGSTFS 180

Query: 181 HHGKLYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELV 240
           HHGK+YQECG ALN+LGMKI+E+LGLSLG+SRE FKNFYEDNDSILRLNYYP CEKPE+V
Sbjct: 181 HHGKVYQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPPCEKPEVV 240

Query: 241 LGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSC 300
           LGTGPH+DPTS+TILHQDHVSGLQV V+DQWYSIPPTPDSFV+NIGDTF SLTNGIYKSC
Sbjct: 241 LGTGPHTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSC 300

Query: 301 LHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRA 360
           +HRAVVNCKE+RKS+AFF+CPAHDKVVRAPEELVEKNPPR Y DYTW MLL+MTQK YRA
Sbjct: 301 VHRAVVNCKEARKSMAFFICPAHDKVVRAPEELVEKNPPRKYPDYTWPMLLEMTQKHYRA 360

Query: 361 DFKTLEAFMARVQKEKSLED--TTSPAA 383
           D  TLEAF   +QKEK L+   T +P+A
Sbjct: 361 DCNTLEAFTTWIQKEKLLDTGCTITPSA 388

[Close]

BLAST of CmoCh18G012280 vs. ExPASy TrEMBL
Match: A0A6J1HXL8 (gibberellin 20 oxidase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467570 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 8.1e-171
Identity = 296/386 (76.68%), Postives = 334/386 (86.53%), Query Frame = 0


Query: 1   MALNCMNATESSPA-LNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHI 60
           MALN   ATES+P+ LN+    EYRP F GSDESK+PEDFIW E+F+A+E++P L VP I
Sbjct: 1   MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60

Query: 61  DLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVK 120
           DL+KF SG++S VEEATRLVDEACR+HG F VVNHGVD+E+M  VH+C++EFFTMPLDVK
Sbjct: 61  DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120

Query: 121 QRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
           QRA+RKVGE+YGYTNSF GRFAS LPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG
Sbjct: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180

Query: 181 KLYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGT 240
           K YQECGIALNELG KIVELLGLSLGISRE FKNFY+DNDSILRLNYYP C+KPE+VLGT
Sbjct: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240

Query: 241 GPHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHR 300
           GPH+DPTS+TILHQD VSGLQV  +DQWYSIPP P++FVINIGDTF SLTNGIYK C+HR
Sbjct: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300

Query: 301 AVVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFK 360
           AVVN   +RKSLAFFLCP+HDKVVRAPEELVEK+PPR Y DY W MLL+MTQK YR D  
Sbjct: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360

Query: 361 TLEAFMARVQKEKSLEDTTSPAATSA 386
           TLEAF   VQ+ K+L+  ++  A SA
Sbjct: 361 TLEAFKTWVQEGKALDTGSTMTAPSA 386

[Close]

BLAST of CmoCh18G012280 vs. ExPASy TrEMBL
Match: A0A6J1HZ92 (gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467571 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 1.1e-170
Identity = 296/386 (76.68%), Postives = 334/386 (86.53%), Query Frame = 0


Query: 1   MALNCMNATESSPA-LNQVRNREYRPAFCGSDESKIPEDFIWPEEFKAAEVVPELHVPHI 60
           MALN   ATES+P+ LN+    EYRP F GSDESK+PEDFIW E+F+A+E++P L VP I
Sbjct: 1   MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60

Query: 61  DLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNHGVDMELMKGVHECIDEFFTMPLDVK 120
           DL+KF SG++S VEEATRLVDEACR+HG F VVNHGVD+E+M  VH+C++EFFTMPLDVK
Sbjct: 61  DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120

Query: 121 QRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
           QRA+RKVGE+YGYTNSF GRFAS LPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG
Sbjct: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180

Query: 181 KLYQECGIALNELGMKIVELLGLSLGISRECFKNFYEDNDSILRLNYYPICEKPELVLGT 240
           K YQECGIALNELG KIVELLGLSLGISRE FKNFY+DNDSILRLNYYP C+KPE+VLGT
Sbjct: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240

Query: 241 GPHSDPTSITILHQDHVSGLQVFVDDQWYSIPPTPDSFVINIGDTFMSLTNGIYKSCLHR 300
           GPH+DPTS+TILHQD VSGLQV  +DQWYSIPP P++FVINIGDTF SLTNGIYK C+HR
Sbjct: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300

Query: 301 AVVNCKESRKSLAFFLCPAHDKVVRAPEELVEKNPPRNYSDYTWEMLLKMTQKDYRADFK 360
           AVVN   +RKSLAFFLCP+HDKVVRAPEELVEK+PPR Y DY W MLL+MTQK YR D  
Sbjct: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360

Query: 361 TLEAFMARVQKEKSLEDTTSPAATSA 386
           TLEAF   VQ+ K+L+  ++  A SA
Sbjct: 361 TLEAFKTWVQEGKALDTGSTITAPSA 386

[Close]

BLAST of CmoCh18G012280 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 406.0 bits (1042), Expect = 3.4e-113
Identity = 194/334 (58.08%), Postives = 240/334 (71.86%), Query Frame = 0


Query: 32  ESKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVV 91
           ++ IP  FIWP++ K +  V EL VP IDL+   S + S   +A+RL+ EAC+KHGFF+V
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96

Query: 92  VNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFS 151
           VNHG+  EL+   HE    FF MPL  KQR  RK GE+ GY +SF GRF++KLPWKET S
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 152 LR-CVAAQNSSAAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 211
            R C     S +  DY  D LG  F   GK+YQE   A++ L +KI+ELLGLSLG+ R+ 
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 212 FKNFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 271
           F+ F+E+NDSI+RLNYYP C KP+L LGTGPH DPTS+TILHQDHV+GLQVFV++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 272 PPTPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 331
            P P +FV+NIGDTFM+L+N  YKSCLHRAVVN +  RKSLAFFLCP  D+VV  P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 332 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 365
           +    R Y D+TW M L+ TQK YRAD  TL+AF
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAF 369

[Close]

BLAST of CmoCh18G012280 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 406.0 bits (1042), Expect = 3.4e-113
Identity = 190/334 (56.89%), Postives = 247/334 (73.95%), Query Frame = 0


Query: 32  ESKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVV 91
           +S+IP  FIWP+E K +  +PEL+VP IDL      ++    EA R++ EAC KHGFF+V
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLV 98

Query: 92  VNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFS 151
           VNHGV   L+   H  ++ FF MPL  KQ+AQRK GE+ GY +SF GRF++KLPWKET S
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 152 LRCVAAQNSS-AAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 211
            +     + S    DY  DTLG  F   GK+YQ+   A++ L +KI+ELLGLSLG++R+ 
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 212 FKNFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 271
           F+ F+E+NDSI+RLN+YP C+ P+L LGTGPH DP+S+TILHQDHV+GLQVFVD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 272 PPTPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 331
            P P +FV+NIGDTFM+L+NGI+KSCLHRAVVN + +RKS+AFFLCP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 332 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 365
           EK   R Y D+TW M L+ TQK YRAD  TL++F
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSF 367

[Close]

BLAST of CmoCh18G012280 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 397.1 bits (1019), Expect = 1.6e-110
Identity = 191/343 (55.69%), Postives = 240/343 (69.97%), Query Frame = 0


Query: 35  IPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNH 94
           IP+ F+WP+  K +  V  L VP IDL  F SG+     EATRLV +A  KHGFF++ NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 95  GVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRC 154
           GVD  L+   +  +D FF  P   KQ+AQRK GE+ GY +SF+GRF+SKLPWKET S + 
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156

Query: 155 VAAQ--NSSAAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGISRECFK 214
              +  +S    D+V   +G  +   GK+YQE   A+N L +KI+ELLG+SLG+ R  FK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216

Query: 215 NFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSIPP 274
            F+ED+DSI RLNYYP C++PEL LGTGPH DPTS+TILHQD V GLQVFVD++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276

Query: 275 TPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELVE- 334
            P +FV+NIGDTFM+LTNG YKSCLHRAVVN +  RK+ AFFLCP  +KVV+ PEELV  
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336

Query: 335 -KNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAFMARVQKEKS 374
            K+  R Y D+TW M L+ TQK YRAD  TL+ F   ++  +S
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRRS 379

[Close]

BLAST of CmoCh18G012280 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 387.5 bits (994), Expect = 1.3e-107
Identity = 188/334 (56.29%), Postives = 231/334 (69.16%), Query Frame = 0


Query: 35  IPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVVVNH 94
           IP++F+WP+  K ++ VP L VP IDL  F S +   V EA RLV EA +KHGFF+V NH
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 95  GVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFSLRC 154
           GVD  L+   H+ +D FF  P   K +AQRKVGE  GY +SF+GRF   LPWKET S   
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 155 VAAQN----SSAAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGISREC 214
              +     S    +Y+  T+G  +   G +YQE    ++ L +KI+ELLG+SLGI RE 
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 215 FKNFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 274
           F+ F+EDN+SI RLNYYP C++P+LVLGTGPH DPTS+TIL QD VSGLQVFVD+QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 275 PPTPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 334
           PP P + V+NIGDT M+LTNGIYKSCLHRAVVN + +RK+LAFFLCP  DKVV+ P EL 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335

Query: 335 EKNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 365
                R Y D+TW M L+ T K YRAD  TLE F
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEF 366

[Close]

BLAST of CmoCh18G012280 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 288.1 bits (736), Expect = 1.0e-77
Identity = 147/335 (43.88%), Postives = 200/335 (59.70%), Query Frame = 0


Query: 32  ESKIPEDFIWPEEFKAAEVVPELHVPHIDLKKFFSGNESDVEEATRLVDEACRKHGFFVV 91
           +  +P +F WPE+   A    +L +P IDL  F +GNE++ + A + V +AC  HG F+V
Sbjct: 40  QPNVPAEFFWPEK-DVAPSEGDLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLV 99

Query: 92  VNHGVDMELMKGVHECIDEFFTMPLDVKQRAQRKVGENYGYTNSFIGRFASKLPWKETFS 151
           VNHG    L +   E    FF +  D K RA R  G   GYT     RF+S LPW ET +
Sbjct: 100 VNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLT 159

Query: 152 LRCVAAQNSSAAHDYVLDTLGSSFSHHGKLYQECGIALNELGMKIVELLGLSLGI-SREC 211
           L            D++   LG+     G+++QE   A+N L M ++ELLG+S+G+  R  
Sbjct: 160 L-AFKKGPPHVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDRTY 219

Query: 212 FKNFYEDNDSILRLNYYPICEKPELVLGTGPHSDPTSITILHQDHVSGLQVFVDDQWYSI 271
           ++ F+ED   I R NYYP C++PE  LG GPH+DPT+IT+L QD V GL+VF    W ++
Sbjct: 220 YRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQTV 279

Query: 272 PPTPDSFVINIGDTFMSLTNGIYKSCLHRAVVNCKESRKSLAFFLCPAHDKVVRAPEELV 331
            P P + V+N+GDTFM+L+NG Y+SC HRAVVN ++ R+SL FF CP  DK++  P ELV
Sbjct: 280 RPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPELV 339

Query: 332 E-KNPPRNYSDYTWEMLLKMTQKDYRADFKTLEAF 365
           E +   R Y D+TW  L K TQ  YR D  TL  F
Sbjct: 340 EGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNF 372

The following BLAST results are available for this feature:

BLAST of CmoCh18G012280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39110	4.8e-112	58.08	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]
Q39111	4.8e-112	56.89	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1	[more]
Q39112	2.2e-109	55.69	Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1	[more]
O04707	4.6e-107	53.30	Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1	[more]
Q9C955	1.8e-106	56.29	Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GA20OX4 PE=2 SV=1	[more]

BLAST of CmoCh18G012280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G194	1.6e-227	100.00	gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449754 PE=3...	[more]
A0A6J1HUH4	2.1e-219	95.84	gibberellin 20 oxidase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467572 PE=3 S...	[more]
A0A6J1G185	1.3e-184	80.93	gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449755 PE=3...	[more]
A0A6J1HXL8	8.1e-171	76.68	gibberellin 20 oxidase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467570 PE=3 S...	[more]
A0A6J1HZ92	1.1e-170	76.68	gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]

BLAST of CmoCh18G012280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25420.1	3.4e-113	58.08	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51810.1	3.4e-113	56.89	gibberellin 20 oxidase 2	[more]
AT5G07200.1	1.6e-110	55.69	gibberellin 20-oxidase 3	[more]
AT1G60980.1	1.3e-107	56.29	gibberellin 20-oxidase 4	[more]
AT1G44090.1	1.0e-77	43.88	gibberellin 20-oxidase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 337..355 score: 23.92 coord: 242..263 score: 44.63 coord: 277..303 score: 30.64 coord: 79..96 score: 56.06
None	No IPR available	PANTHER	PTHR47990:SF26	GIBBERELLIN 20-OXIDASE	coord: 27..366
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 27..366
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 28..356
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 56..160 e-value: 3.5E-24 score: 85.8
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 219..317 e-value: 5.8E-28 score: 97.4
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 26..356 e-value: 3.0E-88 score: 298.3
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 217..317 score: 14.346365

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh18G012280.1	CmoCh18G012280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding