CmoCh18G012060 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh18G012060
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein PTST homolog 2, chloroplastic-like isoform X2
LocationCmo_Chr18: 12027314 .. 12031133 (-)
RNA-Seq ExpressionCmoCh18G012060
SyntenyCmoCh18G012060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGCTGCTGCTTGATTTATCGAACAACTTTCTCGTGAACCAGTGAACCACCACCGCGGTTCTTGTTTCTTCTCCGTCTTTTACCAACCCCCAATTCGATGTAACATATAAATGAAGCCTCCACCAATTACCTTCCCCGCGCCCGAGATTGCCCCTTCAACGTAAGGTTATCTCATTGCTGCGTTTAATTAATGTGGTTTGCATTTCCCACTGGTTTTGTTCGGTTGCTTCGAGAACTGTGTCGAGCCCTTCTTTGTTCTGTCGCCTACTTTTTCTCTTCTCCTTGCGCTCCAGGTGTTCGACGAAATGCCTGAGCCATGCTCCTTTTCACCAACGTATTACCCACTTCTTATTTCTTCCCCACACCGTTTGGATGTTTGAGCCCTGGCGTTTTTCCCTCCATTGTCTTCGTTTCGAGATGCGGCGGGATGGCTGCGACAGAGAGGAGGTGTTCGCTCTTGTCTTCAAAGGGTATGTTTTATGGTTGTTCTGGGTTTGTAAGGAGGTGCAAAGATTTGGATAGGGAAGGGAATTTTGCATTGGAGGCAGAGATATTGGAGTTTATGGAAAGTTCAAGAAACCCAGAAGCGTTTCCGAGCAAGAAAGACTTGATTGAGGCCGGCAGGGAGGATTTAGTGGATGCTATAGTCAAGAAAGGTGGTTGGCTTTGTCTCGGATGGAATTTGAACGACGAAGAAGATGGGGGTATTGAACAGCTCTATCTCGAGGATCGGGAATCTATCTTGGCCACTCATTGGGATAGTTTTGGTGAATTCAATAGTTTCAAGAGTGATGAAAGACAAAGATTTGCTACAGATGTTTCTCCCATTGCTTCTTCTTCGACATCATCTTCCTTCACCAGCCGATCACAGTGAGGATTCCATTATGACATCTTAGTTTATTTCTGCTTCATTTGAAATCACTACTTATAAAAATAACTATTTCCGAATAACGTGCGTGAAAAGGCCATTGGCTTGAAATTTGTTCCTAGCAATCTTCAGGGTGTGTTACAGCATGCCAATGAAAGGAACCCTGAATTTTTACGACAACAAGATGTTGTGTCAGGATGTGAACGACACAAACTTTACTAACAGGTTCATTTAACATGGTTGCCAACGAGCCATCAACCTTAATGTCATTGTCTCATGGAGAAAATTTTAGATGTTTTCTTCATGTTTGAGAAAATGAAGCAAAATAACTTCAAATTTGGGTACCTTAATAATCATTTTAACTGCAGAACATTAATCGAGCTTTCCTCGTTGTGTCATGTTTTGGCTGCTATCAACAATATTTGCAATGGTGATTAGTCTACATGCTTTCATTTTCACAGAGAAACTGTTGTTGAGCACGAGTCTGGGATTGAGGGTATGCTGAATCGATTGGAAAGAGAAAGAAATGTGACGTTCGGGACTGGTAAAAATTGTTTGCATCCTGAAACAACAGTAAAGGAGGTAAGGATGCTCCGGTAATTCAATAAACTGAGTTTTCCAATCTGGGCTAGGTTGTTGTCCGTGTTTAAAGTGTAATTAGAGATTGATTAGAACAGTTTTCATTTCCATAATTCCTGTAGATATAATTTCTCTCATATTAGTAAGTACTCGTTCAAGTAATTTCTTCTGTCTCGAAGATAATTATCGTTAGACTAACCAAGGTAAAAGATATACCTTTGAATTTGATGCAGGATGTCTTTCCCATTGGGTTGTCATAATTAGCCGTGCTTGTGCTATGATTTATTTTTCATTTTTCAACTTTGAAATCGTTACATGTATAATTCTCTTGTAAGAATTGTAAAAGGATGAAACTTGTGTTGCTTCTTGCATGGTAAAAGACAGTTGCTGACCTACAAGAAATCCCTATCAGCACGCCACTGAGTGCCAAAGAAGGCAAGTGGAACAATTTGGGGAGTTATCTTAGTAAGAATTCTTCTCCTGTACAAATTAATGGTCAAGGAGGCTCGGTTAGTTCTGAGGTGCAGGGAACTTGGAGCACATTCAAGACAGACCTTCCAGACGGAGATTTTAAAGGTTTTTCATTTATTCAAGGGTTGCTTTGGGATATAAGAGTTCACATTGACCGACTTTTTATATGTACAGGTTCAGAAATGGCTTTGAGCGAGGGAAGGAGCAGGGAAGTATTGGATGTCCTGAGATATGAAACATTAGGATCTAGAGGGGATCCTACCAATTCTAACTCTGGCGAGGAAGAAATGAGTCACAATCAAATACAACGTCACATTCAGAACTTGCAGTTGGAGCTCTCATCCGTAATCGGCTCATTGAGATCTAATTCATTTGCGTCTGAGAAGGTTTAGTCTGCAACTTGATTTTGTTGTCAAATGAACTGTGACGATCTGCATGTCTTTGATTGAAATGGATGAAATTGATTTATTTCATTTCCTATCATGGAACTGGAGAAGGATCTGAATAAAGTAAAAGATGCTAACTAGTCTAATAGTGTTGATAAAAACGATGAATATCTCTTTGATTGTTGGGCAATAATGTTAAATACGTTAAAATATTGAACCTGCATGTTTATGGTGCTATCATGATCATTAGGGTCAAAGTAAATCTTCTGATGATTTGCTGGAGCTTTCGGATGCTTTTGAGTTTCAAGAAAATGAGATCCTAAATGCCAAGGACAAGCTACGGTCTATACGGGCAAAGATAGCCGTGGTCGAAGGGAAGATGGCACTGACAATTATGTATGGGAAACTTGTGCTTTGCGTAGTGATTTGTTCTATCTATAGAGTATCAATGACTTTCTGTCTTTACAGTGATGCACAAAAGGTGGTAGAAGAGAAACAAAAGAGGATCAATTGTGCCCGCAGAGCTTTGCAATTTCTTCGCACTGCCTGCGTGGTCTGGCCTAATTCTGCAGCAGAGGTTCTCTTAGTAGGATCATTTGATGGTTGGTCAACCCAGGTAAAAAAATTCTTCCTATTTGATTACTTTGCTTGCTTTGATGGTTGAAATACTTGGGGAGGACATGGATTGGTTTCTGTCTATTTCCTATAGAATGTACTGCTTGCTTGCCTGCCTATTGATTGGGGAGGAGAGCAAAAATTCTTTATAAGGACGTGGAAATCTCTCCCCAGCAGACATGTTTTAAAAACCTTGAGGGGAGCCCCGAGAGGGAAAACCCAAAGAGAACAATATCTGCTATTAGTGGGCTTAGGCTTAGGCTGTTACAATTACACTGTAGGTATCATTACGTCAACAGCCTGCATTTTCTAATAGTTAGATGCTACACAAGTTCGATAGTAGGAACAGTAGTATGAAGTGTTGTTAAGGATGTTCATCTGCAAATCGCATTACTGAATAACTCTACAGTTTTATGAGGTTTTATTCATTCATCTCTTGTAGTCTTACTCATATTTAACAACAGCTTATTCTCATTCTGCAGAGGAAGATGGAAAGGTCCAGTACAGGCGTTTTCTCTTTGTTCCTGAAGCTGTATCCTGGAAAATACGAGGTTGGTTGTTCTTTGGAGGTTCTATTCACATAATTCATGCTTTTGAACCATAATCTTACAGTTTTCCATGATATTCAGATAAAATTCATTGTTGATGGCCAGTGGAAGATTGATCCGTTACGCCCAATTGTTCGCAGCGATGGATACGAGAATAATCTACTGATCGTCACGTAGCACACCAGGCTGTTTATATTCTTTTTCTTGCCTAGCCCGAAGAGCCGGGTGCCTCAACTACCCCTTCTTTTGTAGCGTTTTCGTACGAATTATACATGTATTTCTTTCTACTTCCGACATCCAGTTTAGGTTTCTTCCTGTCTCCCCCACGTATTTGCATTTCTCGCTGATGTAA

mRNA sequence

AAGGCTGCTGCTTGATTTATCGAACAACTTTCTCGTGAACCAGTGAACCACCACCGCGGTTCTTGTTTCTTCTCCGTCTTTTACCAACCCCCAATTCGATGTAACATATAAATGAAGCCTCCACCAATTACCTTCCCCGCGCCCGAGATTGCCCCTTCAACGTAAGGTTATCTCATTGCTGCGTTTAATTAATGTGGTTTGCATTTCCCACTGGTTTTGTTCGGTTGCTTCGAGAACTGTGTCGAGCCCTTCTTTGTTCTGTCGCCTACTTTTTCTCTTCTCCTTGCGCTCCAGGTGTTCGACGAAATGCCTGAGCCATGCTCCTTTTCACCAACGTATTACCCACTTCTTATTTCTTCCCCACACCGTTTGGATGTTTGAGCCCTGGCGTTTTTCCCTCCATTGTCTTCGTTTCGAGATGCGGCGGGATGGCTGCGACAGAGAGGAGGTGTTCGCTCTTGTCTTCAAAGGGTATGTTTTATGGTTGTTCTGGGTTTGTAAGGAGGTGCAAAGATTTGGATAGGGAAGGGAATTTTGCATTGGAGGCAGAGATATTGGAGTTTATGGAAAGTTCAAGAAACCCAGAAGCGTTTCCGAGCAAGAAAGACTTGATTGAGGCCGGCAGGGAGGATTTAGTGGATGCTATAGTCAAGAAAGGTGGTTGGCTTTGTCTCGGATGGAATTTGAACGACGAAGAAGATGGGGGTATTGAACAGCTCTATCTCGAGGATCGGGAATCTATCTTGGCCACTCATTGGGATAGTTTTGGTGAATTCAATAGTTTCAAGAGTGATGAAAGACAAAGATTTGCTACAGATGTTTCTCCCATTGCTTCTTCTTCGACATCATCTTCCTTCACCAGCCGATCACAAGAAACTGTTGTTGAGCACGAGTCTGGGATTGAGGGTATGCTGAATCGATTGGAAAGAGAAAGAAATGTGACGTTCGGGACTGGTAAAAATTGTTTGCATCCTGAAACAACAGTAAAGGAGACAGTTGCTGACCTACAAGAAATCCCTATCAGCACGCCACTGAGTGCCAAAGAAGGCAAGTGGAACAATTTGGGGAGTTATCTTAGTAAGAATTCTTCTCCTGTACAAATTAATGGTCAAGGAGGCTCGGTTAGTTCTGAGGTGCAGGGAACTTGGAGCACATTCAAGACAGACCTTCCAGACGGAGATTTTAAAGGTTCAGAAATGGCTTTGAGCGAGGGAAGGAGCAGGGAAGTATTGGATGTCCTGAGATATGAAACATTAGGATCTAGAGGGGATCCTACCAATTCTAACTCTGGCGAGGAAGAAATGAGTCACAATCAAATACAACGTCACATTCAGAACTTGCAGTTGGAGCTCTCATCCGTAATCGGCTCATTGAGATCTAATTCATTTGCGTCTGAGAAGGGTCAAAGTAAATCTTCTGATGATTTGCTGGAGCTTTCGGATGCTTTTGAGTTTCAAGAAAATGAGATCCTAAATGCCAAGGACAAGCTACGGTCTATACGGGCAAAGATAGCCGTGGTCGAAGGGAAGATGGCACTGACAATTATTGATGCACAAAAGGTGGTAGAAGAGAAACAAAAGAGGATCAATTGTGCCCGCAGAGCTTTGCAATTTCTTCGCACTGCCTGCGTGGTCTGGCCTAATTCTGCAGCAGAGGTTCTCTTAGTAGGATCATTTGATGGTTGGTCAACCCAGAGGAAGATGGAAAGGTCCAGTACAGGCGTTTTCTCTTTGTTCCTGAAGCTGTATCCTGGAAAATACGAGATAAAATTCATTGTTGATGGCCAGTGGAAGATTGATCCGTTACGCCCAATTGTTCGCAGCGATGGATACGAGAATAATCTACTGATCGTCACCGTTTTCGTACGAATTATACATGTATTTCTTTCTACTTCCGACATCCAGTTTAGGTTTCTTCCTGTCTCCCCCACGTATTTGCATTTCTCGCTGATGTAA

Coding sequence (CDS)

ATGCTCCTTTTCACCAACGTATTACCCACTTCTTATTTCTTCCCCACACCGTTTGGATGTTTGAGCCCTGGCGTTTTTCCCTCCATTGTCTTCGTTTCGAGATGCGGCGGGATGGCTGCGACAGAGAGGAGGTGTTCGCTCTTGTCTTCAAAGGGTATGTTTTATGGTTGTTCTGGGTTTGTAAGGAGGTGCAAAGATTTGGATAGGGAAGGGAATTTTGCATTGGAGGCAGAGATATTGGAGTTTATGGAAAGTTCAAGAAACCCAGAAGCGTTTCCGAGCAAGAAAGACTTGATTGAGGCCGGCAGGGAGGATTTAGTGGATGCTATAGTCAAGAAAGGTGGTTGGCTTTGTCTCGGATGGAATTTGAACGACGAAGAAGATGGGGGTATTGAACAGCTCTATCTCGAGGATCGGGAATCTATCTTGGCCACTCATTGGGATAGTTTTGGTGAATTCAATAGTTTCAAGAGTGATGAAAGACAAAGATTTGCTACAGATGTTTCTCCCATTGCTTCTTCTTCGACATCATCTTCCTTCACCAGCCGATCACAAGAAACTGTTGTTGAGCACGAGTCTGGGATTGAGGGTATGCTGAATCGATTGGAAAGAGAAAGAAATGTGACGTTCGGGACTGGTAAAAATTGTTTGCATCCTGAAACAACAGTAAAGGAGACAGTTGCTGACCTACAAGAAATCCCTATCAGCACGCCACTGAGTGCCAAAGAAGGCAAGTGGAACAATTTGGGGAGTTATCTTAGTAAGAATTCTTCTCCTGTACAAATTAATGGTCAAGGAGGCTCGGTTAGTTCTGAGGTGCAGGGAACTTGGAGCACATTCAAGACAGACCTTCCAGACGGAGATTTTAAAGGTTCAGAAATGGCTTTGAGCGAGGGAAGGAGCAGGGAAGTATTGGATGTCCTGAGATATGAAACATTAGGATCTAGAGGGGATCCTACCAATTCTAACTCTGGCGAGGAAGAAATGAGTCACAATCAAATACAACGTCACATTCAGAACTTGCAGTTGGAGCTCTCATCCGTAATCGGCTCATTGAGATCTAATTCATTTGCGTCTGAGAAGGGTCAAAGTAAATCTTCTGATGATTTGCTGGAGCTTTCGGATGCTTTTGAGTTTCAAGAAAATGAGATCCTAAATGCCAAGGACAAGCTACGGTCTATACGGGCAAAGATAGCCGTGGTCGAAGGGAAGATGGCACTGACAATTATTGATGCACAAAAGGTGGTAGAAGAGAAACAAAAGAGGATCAATTGTGCCCGCAGAGCTTTGCAATTTCTTCGCACTGCCTGCGTGGTCTGGCCTAATTCTGCAGCAGAGGTTCTCTTAGTAGGATCATTTGATGGTTGGTCAACCCAGAGGAAGATGGAAAGGTCCAGTACAGGCGTTTTCTCTTTGTTCCTGAAGCTGTATCCTGGAAAATACGAGATAAAATTCATTGTTGATGGCCAGTGGAAGATTGATCCGTTACGCCCAATTGTTCGCAGCGATGGATACGAGAATAATCTACTGATCGTCACCGTTTTCGTACGAATTATACATGTATTTCTTTCTACTTCCGACATCCAGTTTAGGTTTCTTCCTGTCTCCCCCACGTATTTGCATTTCTCGCTGATGTAA

Protein sequence

MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVSRCGGMAATERRCSLLSSKGMFYGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSSTSSSFTSRSQETVVEHESGIEGMLNRLERERNVTFGTGKNCLHPETTVKETVADLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVTVFVRIIHVFLSTSDIQFRFLPVSPTYLHFSLM
Homology
BLAST of CmoCh18G012060 vs. ExPASy Swiss-Prot
Match: Q9LFY0 (Protein PTST homolog 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST2 PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 3.3e-90
Identity = 206/476 (43.28%), Postives = 293/476 (61.55%), Query Frame = 0

Query: 57  CSGFVRRCK----DLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVK 116
           C+G  R C       D EG+  LE EILEFM++S  P  FPSKKDLI +GR DLV+ IV 
Sbjct: 72  CNGVSRMCNKRNLGWDSEGSKDLETEILEFMKNSEKPGMFPSKKDLIRSGRFDLVERIVN 131

Query: 117 KGGWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIA 176
           +GGWL +GW+L+++E    E++ + +  +    H +   +  +  S E  +         
Sbjct: 132 QGGWLSMGWDLDEQE----EKVRVNENVTPQDLHIEK--QLPNCNSPEMDKTLNH----G 191

Query: 177 SSSTSSSFTSRSQETVVEHESGIEGMLNRLERERNVTFG-----TGK-NCLHPETTVKET 236
               SS+ +S +++    ++SGIEG+L RLE+ERN++ G      GK N    + +   +
Sbjct: 192 DLDLSSNLSSSTEQVESRNDSGIEGILTRLEKERNLSLGISVRENGKSNGAMHDISPNGS 251

Query: 237 VADLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGG---SVSSEVQGTWSTFKTD 296
           V     I  ++ +   +G   +     S+      ++G+ G   S +SE   TWS  +  
Sbjct: 252 VPWSSRIVTASEIQEVDGSRGSGEYAQSRYQGAKSVSGKPGLSDSPTSETWRTWSMRRAG 311

Query: 297 LPDGDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSN----SGEEEMSHNQIQRHIQ 356
             D DF+ +E++     S  +  V + +T    GD  N      S  E+++   I+  +Q
Sbjct: 312 FTDEDFEAAEIS-----SSGLTGVKKDDTKKDSGDSMNGKDRIASSSEDVNKTHIKHRLQ 371

Query: 357 NLQLELSSVIGSLRS--NSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAK 416
            LQ ELSSV+ SLRS  +   + K    ++ +L  LSD +E++ENEI++A++KLRS RAK
Sbjct: 372 QLQSELSSVLHSLRSPPDKVVTSKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAK 431

Query: 417 IAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWS 476
           +AV+EGKMA+ IIDAQ++V EKQ+RI+ A RAL+ LRTA +VWPNSA+EVLL GSFDGWS
Sbjct: 432 LAVLEGKMAMAIIDAQRIVREKQRRIDHASRALRLLRTASIVWPNSASEVLLTGSFDGWS 491

Query: 477 TQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 514
           TQRKM+++  GVFSL LKLYPGKYEIKFIVDGQWK+DPLRPIV S GYENNLLI++
Sbjct: 492 TQRKMKKAENGVFSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLIIS 532

BLAST of CmoCh18G012060 vs. ExPASy Swiss-Prot
Match: Q10F03 (Protein FLOURY ENDOSPERM 6, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=FLO6 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.1e-64
Identity = 170/467 (36.40%), Postives = 258/467 (55.25%), Query Frame = 0

Query: 71  GNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGWLCLGWNLNDEEDG- 130
           G   LE  I EFM  S  P AFP++ +L+ AGR DL  A+   GGWL LGW+    E G 
Sbjct: 78  GEEDLEEAIYEFMRRSDKPGAFPTRAELVAAGRADLAAAVDACGGWLSLGWSSGGAEAGR 137

Query: 131 -----GIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSSTSSSFTSRS 190
                G+   Y  +  +  A    S     +  +  R+  A        S +     +  
Sbjct: 138 ASSSVGVHPDYPPEAGAAAAAGGASDLAQGAVWASSREAEA--------SPSGRQPETEE 197

Query: 191 QETVVEHESGIEGMLNRLERERNVTFGTGKNCLHPETTVKETVADLQEIPISTPLSAKEG 250
           +ET  +  +G++GML RL+RER                 ++ VA + +    +  SA  G
Sbjct: 198 EETETKFGTGLDGMLTRLQRERERVRPPLPRSSDGAGGERDNVALMGQSGAPSH-SATGG 257

Query: 251 KW-------NNLGSYLSKNSSPVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGSEMALS 310
           ++        N+ SY  +N + ++ N    S++++   TWS  K      DF+ +E+  +
Sbjct: 258 RYTPKVPDNGNIHSYHPQNGA-LEHNKSSKSLTNDAWRTWSLDKGGF--SDFQAAEIHST 317

Query: 311 EGRS---REVLDVLRYETLGSRGDPTNS------NSGEEEMSHNQIQRHIQNLQLELSSV 370
             R     + LD+L  + +     P+N       +  + +   + I   +QNL+L+L++ 
Sbjct: 318 NSRKSFRHDGLDILAQDDVHG---PSNGVAVHDYDINDVDSERDDIHARLQNLELDLTAA 377

Query: 371 IGSLRS--NSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 430
           + +LRS  +   S+  +   +     LSD +EF+E +++ A+++LRSIRAKIAV+EGKMA
Sbjct: 378 LHTLRSRFDKVISDMSEGDGAKAPNGLSDDWEFEETKVMQAQEELRSIRAKIAVLEGKMA 437

Query: 431 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 490
           L II+  K++EEKQ+R++ A +AL  LRT  +VW N A+EVLL GSFDGW++QR+MERS 
Sbjct: 438 LEIIEKNKIIEEKQRRLDEAEKALSELRTVYIVWSNPASEVLLTGSFDGWTSQRRMERSE 497

Query: 491 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 514
            G FSL L+LYPG+YEIKFIVDG W+ DPLRP+V ++G+ENNLL VT
Sbjct: 498 RGTFSLNLRLYPGRYEIKFIVDGVWRNDPLRPLVSNNGHENNLLTVT 529

BLAST of CmoCh18G012060 vs. ExPASy Swiss-Prot
Match: F4KFB3 (Protein PTST homolog 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 4.7e-12
Identity = 44/139 (31.65%), Postives = 71/139 (51.08%), Query Frame = 0

Query: 380 QENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVV 439
           +E E+   K+++   +  ++V++ +    I  AQ ++ EK+  +  A  +L  L+   + 
Sbjct: 457 KELELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEESLSGLQEVVIE 516

Query: 440 WPNSAAEVLLVGSFDGWSTQRKMERSST------GVFSLFLKLYPGKYEIKFIVDGQWKI 499
           +      V + GSF+GW  +  ME  ++        +S  L LYPG YEIKFIVDGQW  
Sbjct: 517 YCGDGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYEIKFIVDGQWIT 576

Query: 500 DPLRPIVRSDGYENNLLIV 513
           DP +  V      NN+L V
Sbjct: 577 DPQKDSVTRGHISNNILKV 595

BLAST of CmoCh18G012060 vs. ExPASy Swiss-Prot
Match: Q94AX2 (Protein PTST, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 5.2e-11
Identity = 41/110 (37.27%), Postives = 63/110 (57.27%), Query Frame = 0

Query: 407 LTIIDA-QKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERS 466
           L+ +DA QK ++E+ K +  A+      +   V W   A  V ++GSFDGWS +  +   
Sbjct: 172 LSRLDAVQKKMKEQIKGVEAAQS-----KEVHVFWIGMAESVQVMGSFDGWSQREDLSPE 231

Query: 467 STGVFSLF---LKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIV 513
            + +F+ F   L L PG+YE+KF+VDG+W+I P  P       ENN+L+V
Sbjct: 232 YSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVV 276

BLAST of CmoCh18G012060 vs. ExPASy Swiss-Prot
Match: Q6PAM0 (5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 7.0e-08
Identity = 34/94 (36.17%), Postives = 50/94 (53.19%), Query Frame = 0

Query: 420 QKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPG 479
           Q+ ++ + +  Q  R   + W     EV + GSF+ WST+  + +S    F   L L  G
Sbjct: 62  QQDLDDSVKPAQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHND-FVAILDLPEG 121

Query: 480 KYEIKFIVDGQWKIDPLRPIVRSD-GYENNLLIV 513
           +++ KF VDGQW  DP  P+V S  G  NNL+ V
Sbjct: 122 EHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHV 154

BLAST of CmoCh18G012060 vs. ExPASy TrEMBL
Match: A0A6J1FZM9 (protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449347 PE=4 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 2.9e-291
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVSRCGGMAATERRCSLLSSKGMFYGCSGF 60
           MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVSRCGGMAATERRCSLLSSKGMFYGCSGF
Sbjct: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVSRCGGMAATERRCSLLSSKGMFYGCSGF 60

Query: 61  VRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGWLCLG 120
           VRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGWLCLG
Sbjct: 61  VRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGWLCLG 120

Query: 121 WNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSSTSSSF 180
           WNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSSTSSSF
Sbjct: 121 WNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSSTSSSF 180

Query: 181 TSRSQETVVEHESGIEGMLNRLERERNVTFGTGKNCLHPETTVKETVADLQEIPISTPLS 240
           TSRSQETVVEHESGIEGMLNRLERERNVTFGTGKNCLHPETTVKETVADLQEIPISTPLS
Sbjct: 181 TSRSQETVVEHESGIEGMLNRLERERNVTFGTGKNCLHPETTVKETVADLQEIPISTPLS 240

Query: 241 AKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGSEMALSEGR 300
           AKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGSEMALSEGR
Sbjct: 241 AKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGSEMALSEGR 300

Query: 301 SREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSLRSNSFASE 360
           SREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSLRSNSFASE
Sbjct: 301 SREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSLRSNSFASE 360

Query: 361 KGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQ 420
           KGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQ
Sbjct: 361 KGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQ 420

Query: 421 KRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGK 480
           KRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGK
Sbjct: 421 KRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGK 480

Query: 481 YEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 514
           YEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT
Sbjct: 481 YEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 513

BLAST of CmoCh18G012060 vs. ExPASy TrEMBL
Match: A0A6J1FZI9 (protein PTST homolog 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449347 PE=4 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 2.2e-286
Identity = 513/544 (94.30%), Postives = 513/544 (94.30%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVSRCGGMAATERRCSLLSSKGMFYGCSGF 60
           MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVSRCGGMAATERRCSLLSSKGMFYGCSGF
Sbjct: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVSRCGGMAATERRCSLLSSKGMFYGCSGF 60

Query: 61  VRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGWLCLG 120
           VRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGWLCLG
Sbjct: 61  VRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGWLCLG 120

Query: 121 WNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSSTSSSF 180
           WNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSSTSSSF
Sbjct: 121 WNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSSTSSSF 180

Query: 181 TSRSQ-------------------------------ETVVEHESGIEGMLNRLERERNVT 240
           TSRSQ                               ETVVEHESGIEGMLNRLERERNVT
Sbjct: 181 TSRSQVCYSMPMKGTLNFYDNKMLCQDVNDTNFTNRETVVEHESGIEGMLNRLERERNVT 240

Query: 241 FGTGKNCLHPETTVKETVADLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSV 300
           FGTGKNCLHPETTVKETVADLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSV
Sbjct: 241 FGTGKNCLHPETTVKETVADLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSV 300

Query: 301 SSEVQGTWSTFKTDLPDGDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEM 360
           SSEVQGTWSTFKTDLPDGDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEM
Sbjct: 301 SSEVQGTWSTFKTDLPDGDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEM 360

Query: 361 SHNQIQRHIQNLQLELSSVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKD 420
           SHNQIQRHIQNLQLELSSVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKD
Sbjct: 361 SHNQIQRHIQNLQLELSSVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKD 420

Query: 421 KLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLL 480
           KLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLL
Sbjct: 421 KLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLL 480

Query: 481 VGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNL 514
           VGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNL
Sbjct: 481 VGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNL 540

BLAST of CmoCh18G012060 vs. ExPASy TrEMBL
Match: A0A6J1HXH5 (protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467545 PE=4 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.7e-275
Identity = 493/517 (95.36%), Postives = 495/517 (95.74%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVSRCGG----MAATERRCSLLSSKGMFYG 60
           MLLFTN LPTSYFFPTPFGCLSP  FPS VFVSRCGG    MAATERRCSLLSSKGMFYG
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFVSRCGGNSYHMAATERRCSLLSSKGMFYG 60

Query: 61  CSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGW 120
           CSGFVRRCK LD EGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGW
Sbjct: 61  CSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGW 120

Query: 121 LCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSST 180
           LCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSST
Sbjct: 121 LCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSST 180

Query: 181 SSSFTSRSQETVVEHESGIEGMLNRLERERNVTFGTGKNCLHPETTVKETVADLQEIPIS 240
           SSSFTSRSQETV EHESGIEGMLNRLERERN TFGTGKN LH ETT KETVADLQEIPIS
Sbjct: 181 SSSFTSRSQETVAEHESGIEGMLNRLERERNATFGTGKNGLHSETTAKETVADLQEIPIS 240

Query: 241 TPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGSEMAL 300
           TPLSAKEGKW NLGSYLSKNSSPVQINGQGGSVSSEVQ TWSTFKTDL DGDFKGSEMAL
Sbjct: 241 TPLSAKEGKWTNLGSYLSKNSSPVQINGQGGSVSSEVQRTWSTFKTDLQDGDFKGSEMAL 300

Query: 301 SEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSLRSNS 360
           SE RSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQR+IQNLQLELSS IGSLRSN+
Sbjct: 301 SEVRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRYIQNLQLELSSAIGSLRSNA 360

Query: 361 FASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVV 420
           FASEKGQSK SDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVV
Sbjct: 361 FASEKGQSKPSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVV 420

Query: 421 EEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKL 480
           EEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKL
Sbjct: 421 EEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKL 480

Query: 481 YPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 514
           YPGKYEIKFIVDGQWKIDPLRPIV SDGYENNLLIVT
Sbjct: 481 YPGKYEIKFIVDGQWKIDPLRPIVSSDGYENNLLIVT 517

BLAST of CmoCh18G012060 vs. ExPASy TrEMBL
Match: A0A6J1HT44 (protein PTST homolog 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467545 PE=4 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 493/548 (89.96%), Postives = 495/548 (90.33%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFVSRCGG----MAATERRCSLLSSKGMFYG 60
           MLLFTN LPTSYFFPTPFGCLSP  FPS VFVSRCGG    MAATERRCSLLSSKGMFYG
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFVSRCGGNSYHMAATERRCSLLSSKGMFYG 60

Query: 61  CSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGW 120
           CSGFVRRCK LD EGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGW
Sbjct: 61  CSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKGGW 120

Query: 121 LCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSST 180
           LCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSST
Sbjct: 121 LCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASSST 180

Query: 181 SSSFTSRSQ-------------------------------ETVVEHESGIEGMLNRLERE 240
           SSSFTSRSQ                               ETV EHESGIEGMLNRLERE
Sbjct: 181 SSSFTSRSQVFYSMPMKRNLIFYDNKMLCQDVKDTNFTNRETVAEHESGIEGMLNRLERE 240

Query: 241 RNVTFGTGKNCLHPETTVKETVADLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQ 300
           RN TFGTGKN LH ETT KETVADLQEIPISTPLSAKEGKW NLGSYLSKNSSPVQINGQ
Sbjct: 241 RNATFGTGKNGLHSETTAKETVADLQEIPISTPLSAKEGKWTNLGSYLSKNSSPVQINGQ 300

Query: 301 GGSVSSEVQGTWSTFKTDLPDGDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSG 360
           GGSVSSEVQ TWSTFKTDL DGDFKGSEMALSE RSREVLDVLRYETLGSRGDPTNSNSG
Sbjct: 301 GGSVSSEVQRTWSTFKTDLQDGDFKGSEMALSEVRSREVLDVLRYETLGSRGDPTNSNSG 360

Query: 361 EEEMSHNQIQRHIQNLQLELSSVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEIL 420
           EEEMSHNQIQR+IQNLQLELSS IGSLRSN+FASEKGQSK SDDLLELSDAFEFQENEIL
Sbjct: 361 EEEMSHNQIQRYIQNLQLELSSAIGSLRSNAFASEKGQSKPSDDLLELSDAFEFQENEIL 420

Query: 421 NAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAA 480
           NAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAA
Sbjct: 421 NAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAA 480

Query: 481 EVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGY 514
           EVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV SDGY
Sbjct: 481 EVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVSSDGY 540

BLAST of CmoCh18G012060 vs. ExPASy TrEMBL
Match: A0A6J1DCK2 (uncharacterized protein LOC111019111 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019111 PE=4 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 1.1e-216
Identity = 398/519 (76.69%), Postives = 439/519 (84.59%), Query Frame = 0

Query: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFV--SRCG----GMAATERRCSLLSSKGMF 60
           ML+FTN LPT  FFP+PFGCLSP VFP++VFV  SRCG      AATER C LLSSKGMF
Sbjct: 1   MLVFTNALPTFLFFPSPFGCLSPCVFPNVVFVSNSRCGWNSHHTAATERGCVLLSSKGMF 60

Query: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120
           YGCSG VRRCK LD EG+FALEA+ILEFM+SS+NPEAFPSKKDLIEAGREDLVDAI KKG
Sbjct: 61  YGCSGVVRRCKGLDSEGDFALEAKILEFMKSSKNPEAFPSKKDLIEAGREDLVDAIAKKG 120

Query: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180
           GWLCLGWNL++E D G+ Q   EDR SI+AT WDSFGE N+ + DER RFA   SP+ASS
Sbjct: 121 GWLCLGWNLDEEVDEGVGQYDFEDRYSIVATDWDSFGESNNSRRDERPRFAAAASPLASS 180

Query: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNVTFGTGKNCLHPETTVKETVADLQEIP 240
           S SSSFT RS E V E ESGIEGML+RLE+ERNVTFG G          KETVADLQ   
Sbjct: 181 SASSSFTGRSLEIVAEGESGIEGMLSRLEKERNVTFGAG------FPGAKETVADLQGSS 240

Query: 241 ISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQGTWSTFKTDLPDGDFKGSEM 300
           ISTPLSAKEGKWNNLG  LS NSSPVQING GGSVS EVQ  W+T+ T+L DGDF+ SE 
Sbjct: 241 ISTPLSAKEGKWNNLGGSLSHNSSPVQINGLGGSVSPEVQRIWNTYNTELRDGDFEASEN 300

Query: 301 ALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELSSVIGSLRS 360
           AL  GR  EVLDV RY TLGS GD   SN GEEEM+++QI+RH+QNL+LELSS++GSLRS
Sbjct: 301 ALRNGRGGEVLDVSRYGTLGSGGDLIKSNPGEEEMNYHQIRRHLQNLELELSSMVGSLRS 360

Query: 361 NSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQK 420
           N+FASEKGQS+S+DD LELSDA+EFQENEILNAKDKLRSIRAK+AVVEGKMA+TIIDAQK
Sbjct: 361 NTFASEKGQSRSADDFLELSDAYEFQENEILNAKDKLRSIRAKLAVVEGKMAMTIIDAQK 420

Query: 421 VVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFL 480
           V+EEKQKRINCARRALQ LRTACVVWPNSA+EV LVGSFDGW+TQRKME+SSTGVFSLFL
Sbjct: 421 VIEEKQKRINCARRALQVLRTACVVWPNSASEVFLVGSFDGWATQRKMEKSSTGVFSLFL 480

Query: 481 KLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 514
           KLYPGKYEIKFIVDGQW+IDPLRPIV S GYENNLLI+T
Sbjct: 481 KLYPGKYEIKFIVDGQWRIDPLRPIVNSSGYENNLLIIT 513

BLAST of CmoCh18G012060 vs. TAIR 10
Match: AT1G27070.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 334.0 bits (855), Expect = 2.3e-91
Identity = 206/476 (43.28%), Postives = 293/476 (61.55%), Query Frame = 0

Query: 57  CSGFVRRCK----DLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVK 116
           C+G  R C       D EG+  LE EILEFM++S  P  FPSKKDLI +GR DLV+ IV 
Sbjct: 72  CNGVSRMCNKRNLGWDSEGSKDLETEILEFMKNSEKPGMFPSKKDLIRSGRFDLVERIVN 131

Query: 117 KGGWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIA 176
           +GGWL +GW+L+++E    E++ + +  +    H +   +  +  S E  +         
Sbjct: 132 QGGWLSMGWDLDEQE----EKVRVNENVTPQDLHIEK--QLPNCNSPEMDKTLNH----G 191

Query: 177 SSSTSSSFTSRSQETVVEHESGIEGMLNRLERERNVTFG-----TGK-NCLHPETTVKET 236
               SS+ +S +++    ++SGIEG+L RLE+ERN++ G      GK N    + +   +
Sbjct: 192 DLDLSSNLSSSTEQVESRNDSGIEGILTRLEKERNLSLGISVRENGKSNGAMHDISPNGS 251

Query: 237 VADLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGG---SVSSEVQGTWSTFKTD 296
           V     I  ++ +   +G   +     S+      ++G+ G   S +SE   TWS  +  
Sbjct: 252 VPWSSRIVTASEIQEVDGSRGSGEYAQSRYQGAKSVSGKPGLSDSPTSETWRTWSMRRAG 311

Query: 297 LPDGDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSN----SGEEEMSHNQIQRHIQ 356
             D DF+ +E++     S  +  V + +T    GD  N      S  E+++   I+  +Q
Sbjct: 312 FTDEDFEAAEIS-----SSGLTGVKKDDTKKDSGDSMNGKDRIASSSEDVNKTHIKHRLQ 371

Query: 357 NLQLELSSVIGSLRS--NSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAK 416
            LQ ELSSV+ SLRS  +   + K    ++ +L  LSD +E++ENEI++A++KLRS RAK
Sbjct: 372 QLQSELSSVLHSLRSPPDKVVTSKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAK 431

Query: 417 IAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWS 476
           +AV+EGKMA+ IIDAQ++V EKQ+RI+ A RAL+ LRTA +VWPNSA+EVLL GSFDGWS
Sbjct: 432 LAVLEGKMAMAIIDAQRIVREKQRRIDHASRALRLLRTASIVWPNSASEVLLTGSFDGWS 491

Query: 477 TQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 514
           TQRKM+++  GVFSL LKLYPGKYEIKFIVDGQWK+DPLRPIV S GYENNLLI++
Sbjct: 492 TQRKMKKAENGVFSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLIIS 532

BLAST of CmoCh18G012060 vs. TAIR 10
Match: AT5G03420.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 74.3 bits (181), Expect = 3.3e-13
Identity = 44/139 (31.65%), Postives = 71/139 (51.08%), Query Frame = 0

Query: 380 QENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRTACVV 439
           +E E+   K+++   +  ++V++ +    I  AQ ++ EK+  +  A  +L  L+   + 
Sbjct: 457 KELELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEESLSGLQEVVIE 516

Query: 440 WPNSAAEVLLVGSFDGWSTQRKMERSST------GVFSLFLKLYPGKYEIKFIVDGQWKI 499
           +      V + GSF+GW  +  ME  ++        +S  L LYPG YEIKFIVDGQW  
Sbjct: 517 YCGDGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYEIKFIVDGQWIT 576

Query: 500 DPLRPIVRSDGYENNLLIV 513
           DP +  V      NN+L V
Sbjct: 577 DPQKDSVTRGHISNNILKV 595

BLAST of CmoCh18G012060 vs. TAIR 10
Match: AT5G39790.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 70.9 bits (172), Expect = 3.7e-12
Identity = 41/110 (37.27%), Postives = 63/110 (57.27%), Query Frame = 0

Query: 407 LTIIDA-QKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERS 466
           L+ +DA QK ++E+ K +  A+      +   V W   A  V ++GSFDGWS +  +   
Sbjct: 168 LSRLDAVQKKMKEQIKGVEAAQS-----KEVHVFWIGMAESVQVMGSFDGWSQREDLSPE 227

Query: 467 STGVFSLF---LKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIV 513
            + +F+ F   L L PG+YE+KF+VDG+W+I P  P       ENN+L+V
Sbjct: 228 YSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVV 272

BLAST of CmoCh18G012060 vs. TAIR 10
Match: AT4G16360.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-08
Identity = 29/80 (36.25%), Postives = 46/80 (57.50%), Query Frame = 0

Query: 438 VVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLR 497
           + W +   E+ + GS+D W T+ +++RS    F++   L  G YE +FIVDGQW+  P  
Sbjct: 76  ITWCHGGKEIAVEGSWDNWKTRSRLQRSGKD-FTIMKVLPSGVYEYRFIVDGQWRHAPEL 135

Query: 498 PIVRSD-GYENNLLIVTVFV 517
           P+ R D G   N+L +  +V
Sbjct: 136 PLARDDAGNTFNILDLQDYV 154

BLAST of CmoCh18G012060 vs. TAIR 10
Match: AT4G16360.3 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-08
Identity = 29/80 (36.25%), Postives = 46/80 (57.50%), Query Frame = 0

Query: 438 VVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLR 497
           + W +   E+ + GS+D W T+ +++RS    F++   L  G YE +FIVDGQW+  P  
Sbjct: 78  ITWCHGGKEIAVEGSWDNWKTRSRLQRSGKD-FTIMKVLPSGVYEYRFIVDGQWRHAPEL 137

Query: 498 PIVRSD-GYENNLLIVTVFV 517
           P+ R D G   N+L +  +V
Sbjct: 138 PLARDDAGNTFNILDLQDYV 156

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFY03.3e-9043.28Protein PTST homolog 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST2 P... [more]
Q10F031.1e-6436.40Protein FLOURY ENDOSPERM 6, chloroplastic OS=Oryza sativa subsp. japonica OX=399... [more]
F4KFB34.7e-1231.65Protein PTST homolog 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE... [more]
Q94AX25.2e-1137.27Protein PTST, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1[more]
Q6PAM07.0e-0836.175'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab... [more]
Match NameE-valueIdentityDescription
A0A6J1FZM92.9e-291100.00protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3... [more]
A0A6J1FZI92.2e-28694.30protein PTST homolog 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1HXH51.7e-27595.36protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=366... [more]
A0A6J1HT441.3e-27089.96protein PTST homolog 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1DCK21.1e-21676.69uncharacterized protein LOC111019111 isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT1G27070.12.3e-9143.285'-AMP-activated protein kinase-related [more]
AT5G03420.13.3e-1331.655'-AMP-activated protein kinase-related [more]
AT5G39790.13.7e-1237.275'-AMP-activated protein kinase-related [more]
AT4G16360.11.4e-0836.255'-AMP-activated protein kinase beta-2 subunit protein [more]
AT4G16360.31.4e-0836.255'-AMP-activated protein kinase beta-2 subunit protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 370..397
NoneNo IPR availableCOILSCoilCoilcoord: 409..436
NoneNo IPR availablePANTHERPTHR47434:SF1PROTEIN PTST HOMOLOG 2, CHLOROPLASTICcoord: 40..513
NoneNo IPR availablePANTHERPTHR47434PROTEIN PTST HOMOLOG 3, CHLOROPLASTICcoord: 40..513
NoneNo IPR availableCDDcd02859E_set_AMPKbeta_like_Ncoord: 438..512
e-value: 4.57965E-26
score: 99.5956
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 433..514
e-value: 4.7E-22
score: 79.8
IPR032640AMP-activated protein kinase, glycogen-binding domainPFAMPF16561AMPK1_CBMcoord: 438..512
e-value: 5.7E-18
score: 64.9
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 436..512

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh18G012060.1CmoCh18G012060.1mRNA