CmoCh18G008480 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh18G008480
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionAlpha-galactosidase
LocationCmo_Chr18: 9819302 .. 9822205 (-)
RNA-Seq ExpressionCmoCh18G008480
SyntenyCmoCh18G008480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAGAGAAAAGGTCTAGAAGCTCTCTATTTATACCTAAAACTCCACCGCTCCTTCTTCTCTCTCTCTCTCTCTCGCTTCGGATCAAATGGAGACACTGGCCGCCGCCATTGCTTCGATTCTGCTCTCCTTCCTTCTGATTCCGCCTTCCGCCGCCGCTTCTCGCAAGCTCCGCCCTCAAGACCGCCGGAATCTCCTCGCCAATGCTCTCGCCTCCACTCCTCCCATGGGGTTCCAACTCCCTTTCCTCCTCCTTTAATTTCTCTTTAATTTCTTTCTATTCATTTTTTCGCTTTGTTTTGTAGATGGAACAGTTGGAATCACTTCCGGTGCGGCATTAGCGAGACCATCGTCAAGCAAAACGGTGTCGTTTTCATTTACTCTCTTTATTTTCTAATATTTATTAATCGTTTTTTCTTTTTTTAATAATTAAATAAATAAAATAATTCTACAGCTCATCTTCTTGTTTCCACTGGCCTCTCCAAGCTTGGATACGTCTACGTTAACATAGGTAAAAATAAAATTTAAAATATTTTTATTTTTTTTTCTTAGTAATTGTAAATTTGTTTTTACCCGACTAAAACGCAGTCGTTTTTAGATGATTAAATAAACAAAGAATATTCTTTCCATTGATTTGAGGTCTTTTAGAGAAGCACAAAGCAAAACCATGAAAGTTTATGCTCAAAATAGACAATACATACTATTGTGGAGAGTCGTGTTCGTCTAACATGATCAAAACCATGTCCTAAACTTAGTTTATGCCAATAGAATGGAGTCGCCTCCTCGAAGGCCGTCAAAAAATGACTAGGGCTCCAAGGAGTCTAGCCTCGATTAAGGGGAGGTGTTTGGAGGTGTTACACGATGGAAATCCCACGTAGCTTACTCATTGTGGTGCGGAGAGTCTGTTAAACATAAATAATAAATAAATATATTTTTTTTTGCTTGGGATATTTTAAATATAAATTATATAAAAAATGGAAATTATTCATTAAAAATTTTAAAATATATATATTTTTTAACTGTAAATTTTTTTATTAAATTATTTGAAAAATTGCACCAAATAGTATTATTATTATTATTATTTTAATTTAATATAAATTTTGTAGGGATATTTGGTGCCAAAGAAGGCCACATTTCCATCTGGAATTAAATCCCTAGCAGATTACGTTCATAACAAGGGCTTAAAGCTTGGAATTTACTCTGATTCCGGGTAAATCCCACCCTCTTTCATCTTCCTTTTTTCCCTTTTTCCAATTTTAACCCCACGGTGAATTTCCCTCCAGCACATTTACCTGCAGTAAAACCATGCCCGGTTCACTATACCACGAAGAACAAGACGCCAAAACCTTCGCCAACTGGGTATATAAATTAAAACGCCTTTTTATTATAATTATTTATAAATTAATTGGAAATTCATTATTATTTGTTTTAAATTAATAGGGAGTTGACTATTTAAAGTATGATAACTGTAACAACGACAACATTAAGCCAACGGAGAGGTGGGCATTATGGTAATTTAGACACTTTTTTATTTTTAAAAACCCAAAACTCAAAATTTATTAATTTAATTTCCTCTCTTTTTAAAGATATCCCATAATGACAAGAGCTCTGATGAAGGCAGGACGGCCCATTTTCCTATCGCTATGCGAAGGGTAAATTAAATACTTTATTATATTATAACATTTATTTTATTTTATTTTTAATTATAATTAATTCCTAATCTTACGTTTAAAACAGTGGCGACATGCATCCTGCCATGTGGGGTGCTGACGTGGCAAACAGTTGGAGAACCACCAGTGACATCGAGGACAATTGGGACAGGTAGGATTATAGTTTTAATTTATATTAATTAATTAATTCTCATTTAATAATTTATTTTATTTTAACAGATGAGCAGAGCCGACTTGAACGATGCCTTTGCAGAGTATGCAAGGCCTGGTGGTTGGAATGGTCAGTTTTATTAATAAATAAATAATTAATTATATATGTGTATATATATTAAAAATATATATTTAATTATTTAATTATTAGATCCTGACATGCTTGAAGTTGGAAATGGAGGGATGACTAAGGAAGAGTACAGAGTCCACTTCAGCATATGGGCCATTTCTAAGGTCATTTCCTCAGGCCCAAAGCAATAAAAATAATAATTTTTTATTTTATTTTTTTAATTTTTATTTATTTTTACAAAATTGGCCTCAGGCTCCACTTCTCTTGGGTAATGATCTGAGGGAGATTTCCGAGGACCCAATGGAAATAATTGGGAATTCTGAGGTTATTTCTGTAAATCAAGGTAAATTTAATTCTATTATTATTATTTGGAATTATATTTAAAAAAAACATTTTTGGACTAAAATTGGTTACGTTTTGGTAGATCCTCTGGGTGTCCAAGCTAAGAAGGTTAGAAGCGGACACGTGGAGGTAATTAAAATAGACAAATTTATTAATTATTATTTTTTAAATTTAGAGATAATAAATTAAAAAAAGGGTAAAAAAAAAAAATGGAACAGGTTTGGGCTGGGCCTTTATCTGGATACAGAGTGGCGGTTCTGTTGGTGAACAGAAGGAAATGGAGGATGGCAGGTAGGGCCTATTGGGACGACATCGGCATTCCTAACGACACTGTCGTTCAGGCGAGAGATCTCCTATTATAGTTTTGTCGGGAGAGTTATCTCGTACCCTTAGGTATTTTCTACATACCCACCTGTGTCGGTTTTAGATACAGGTACCCTTTTGTTGAAAGTCGTTCGAGCTTTTCCTGAGAATATGGCATGAGTTACTTTAGCGAGCGCATGGTAATCAAACATTGGTGAGAGGTATTTTCTACATACCCACCTGTGTCGGTTTTATATACAGGTACCCTTTTGTTGAAGTCGTTCGAGCTTTTCCTGGGAATATGGCATGA

mRNA sequence

TGAAGAGAAAAGGTCTAGAAGCTCTCTATTTATACCTAAAACTCCACCGCTCCTTCTTCTCTCTCTCTCTCTCTCGCTTCGGATCAAATGGAGACACTGGCCGCCGCCATTGCTTCGATTCTGCTCTCCTTCCTTCTGATTCCGCCTTCCGCCGCCGCTTCTCGCAAGCTCCGCCCTCAAGACCGCCGGAATCTCCTCGCCAATGCTCTCGCCTCCACTCCTCCCATGGGATGGAACAGTTGGAATCACTTCCGGTGCGGCATTAGCGAGACCATCGTCAAGCAAAACGCTCATCTTCTTGTTTCCACTGGCCTCTCCAAGCTTGGATACGTCTACGTTAACATAGATTACGTTCATAACAAGGGCTTAAAGCTTGGAATTTACTCTGATTCCGGCACATTTACCTGCAGTAAAACCATGCCCGGTTCACTATACCACGAAGAACAAGACGCCAAAACCTTCGCCAACTGGGGAGTTGACTATTTAAAGTATGATAACTGTAACAACGACAACATTAAGCCAACGGAGAGATATCCCATAATGACAAGAGCTCTGATGAAGGCAGGACGGCCCATTTTCCTATCGCTATGCGAAGGTGGCGACATGCATCCTGCCATGTGGGGTGCTGACGTGGCAAACAGTTGGAGAACCACCAGTGACATCGAGGACAATTGGGACAGAGCCGACTTGAACGATGCCTTTGCAGAGTATGCAAGGCCTGGTGGTTGGAATGATCCTGACATGCTTGAAGTTGGAAATGGAGGGATGACTAAGGAAGAGTACAGAGTCCACTTCAGCATATGGGCCATTTCTAAGGCTCCACTTCTCTTGGGTAATGATCTGAGGGAGATTTCCGAGGACCCAATGGAAATAATTGGGAATTCTGAGGTTATTTCTGTAAATCAAGATCCTCTGGGTGTCCAAGCTAAGAAGGTTAGAAGCGGACACGTGGAGGTTTGGGCTGGGCCTTTATCTGGATACAGAGTGGCGGTTCTGTTGGTGAACAGAAGGAAATGGAGGATGGCAGTTTTGTCGGGAGAGTTATCTCGTACCCTTAGGTACCCTTTTGTTGAAGTCGTTCGAGCTTTTCCTGGGAATATGGCATGA

Coding sequence (CDS)

ATGGAGACACTGGCCGCCGCCATTGCTTCGATTCTGCTCTCCTTCCTTCTGATTCCGCCTTCCGCCGCCGCTTCTCGCAAGCTCCGCCCTCAAGACCGCCGGAATCTCCTCGCCAATGCTCTCGCCTCCACTCCTCCCATGGGATGGAACAGTTGGAATCACTTCCGGTGCGGCATTAGCGAGACCATCGTCAAGCAAAACGCTCATCTTCTTGTTTCCACTGGCCTCTCCAAGCTTGGATACGTCTACGTTAACATAGATTACGTTCATAACAAGGGCTTAAAGCTTGGAATTTACTCTGATTCCGGCACATTTACCTGCAGTAAAACCATGCCCGGTTCACTATACCACGAAGAACAAGACGCCAAAACCTTCGCCAACTGGGGAGTTGACTATTTAAAGTATGATAACTGTAACAACGACAACATTAAGCCAACGGAGAGATATCCCATAATGACAAGAGCTCTGATGAAGGCAGGACGGCCCATTTTCCTATCGCTATGCGAAGGTGGCGACATGCATCCTGCCATGTGGGGTGCTGACGTGGCAAACAGTTGGAGAACCACCAGTGACATCGAGGACAATTGGGACAGAGCCGACTTGAACGATGCCTTTGCAGAGTATGCAAGGCCTGGTGGTTGGAATGATCCTGACATGCTTGAAGTTGGAAATGGAGGGATGACTAAGGAAGAGTACAGAGTCCACTTCAGCATATGGGCCATTTCTAAGGCTCCACTTCTCTTGGGTAATGATCTGAGGGAGATTTCCGAGGACCCAATGGAAATAATTGGGAATTCTGAGGTTATTTCTGTAAATCAAGATCCTCTGGGTGTCCAAGCTAAGAAGGTTAGAAGCGGACACGTGGAGGTTTGGGCTGGGCCTTTATCTGGATACAGAGTGGCGGTTCTGTTGGTGAACAGAAGGAAATGGAGGATGGCAGTTTTGTCGGGAGAGTTATCTCGTACCCTTAGGTACCCTTTTGTTGAAGTCGTTCGAGCTTTTCCTGGGAATATGGCATGA

Protein sequence

METLAAAIASILLSFLLIPPSAAASRKLRPQDRRNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNIDYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDAKTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADVANSWRTTSDIEDNWDRADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVRSGHVEVWAGPLSGYRVAVLLVNRRKWRMAVLSGELSRTLRYPFVEVVRAFPGNMA
Homology
BLAST of CmoCh18G008480 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 8.5e-121
Identity = 212/336 (63.10%), Postives = 249/336 (74.11%), Query Frame = 0

Query: 33  RRNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI------ 92
           RR+LL N L  TPPMGWNSWNHF C I E ++K+ A  LV+TGLSKLGY YVNI      
Sbjct: 42  RRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNIDDCWAE 101

Query: 93  ------------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDA 152
                                   DYVH+KGLKLGIYSD+G FTCSKTMPGSL +EE DA
Sbjct: 102 ISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDA 161

Query: 153 KTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADV 212
           KTFA WG+DYLKYDNCN+D  KPT RYP+MTRALMK+GRPIF SLCE GDMHPA+WG+ V
Sbjct: 162 KTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPALWGSPV 221

Query: 213 ANSWRTTSDIEDNW----DRADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSI 272
            NSWRTT+DI+D W      AD+N+ +AE+ARPGGWNDPDMLEVGNGGMTK+EY VHFSI
Sbjct: 222 GNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYIVHFSI 281

Query: 273 WAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVR-SGHVEVWAGPLSG 332
           WAISKAPLLLG D+R ++++ MEI+ N EVI++NQDP GVQAKKVR  G +EVWAGPLSG
Sbjct: 282 WAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWAGPLSG 341

Query: 333 YRVAVLLVNRRKWRMAVLSGELSRTLRYPFVEVVRA 334
           YRVA+LL+NR   R ++ +  L   +  P   +V A
Sbjct: 342 YRVALLLLNRGPSRTSITA--LWEDIEIPANSIVEA 375

BLAST of CmoCh18G008480 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 8.8e-110
Identity = 189/310 (60.97%), Postives = 226/310 (72.90%), Query Frame = 0

Query: 33  RRNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI------ 92
           RR+LLAN L  TPPMGWNSWNHFRC + E ++++ A  +VS GL+ LGY Y+N+      
Sbjct: 12  RRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCWAE 71

Query: 93  ------------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDA 152
                                   DYVH+KGLKLGIYSD+GT TCSKTMPGSL HEEQDA
Sbjct: 72  LNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEEQDA 131

Query: 153 KTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADV 212
           KTFA+WGVDYLKYDNCNN+NI P ERYPIM++AL+ +GR IF SLCE G+  PA W  +V
Sbjct: 132 KTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWAKEV 191

Query: 213 ANSWRTTSDIEDNW----DRADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSI 272
            NSWRTT DI+D+W     RAD+ND +A YA PGGWNDPDMLEVGNGGMT  EYR HFSI
Sbjct: 192 GNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSHFSI 251

Query: 273 WAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVRS-GHVEVWAGPLSG 308
           WA++KAPLL+G D+R +     +++ N+EVI+VNQD LGVQ  KV++ G +EVWAGPLSG
Sbjct: 252 WALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGPLSG 311

BLAST of CmoCh18G008480 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.0e-106
Identity = 185/309 (59.87%), Postives = 219/309 (70.87%), Query Frame = 0

Query: 34  RNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI------- 93
           R L+ N LA +P MGWNSWNHF+C I+ET++KQ A  +VS+GLS +GY Y+NI       
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 88

Query: 94  -----------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDAK 153
                                  DYVH+KGLKLGIYSD+GT TCS+TMPGSL HEEQDAK
Sbjct: 89  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 148

Query: 154 TFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADVA 213
           TFA+WG+DYLKYDNC N    P ERYP M++AL+ +GR IF SLCE G   PA W  D+ 
Sbjct: 149 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 208

Query: 214 NSWRTTSDIEDNWDR----ADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSIW 273
           NSWRTT DI+DNW      AD ND +A YARPG WNDPDMLEVGNGGMTKEEY  HFSIW
Sbjct: 209 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 268

Query: 274 AISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKV-RSGHVEVWAGPLSGY 308
           A++KAPLL+G DLR + +   E++ N EVI+VNQD LG+Q KKV + G +EVWAGPLS  
Sbjct: 269 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 328

BLAST of CmoCh18G008480 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.4e-104
Identity = 185/317 (58.36%), Postives = 218/317 (68.77%), Query Frame = 0

Query: 33  RRNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI------ 92
           RR L  N L  TPPMGWNSWNHF C I+E +V++ A  +VSTGL+ LGY Y+N+      
Sbjct: 44  RRYLAENGLGQTPPMGWNSWNHFGCDINENVVRETADAMVSTGLAALGYQYINLDDCWAE 103

Query: 93  ------------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDA 152
                                   DYVH+KGLKLG+YSD+G  TCSK MPGSL HEEQDA
Sbjct: 104 LNRDSEGNMVPNAAAFPSGIKALADYVHSKGLKLGVYSDAGNQTCSKRMPGSLGHEEQDA 163

Query: 153 KTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADV 212
           KTFA+WGVDYLKYDNC N  I   ERYP M +AL+ +GRPIF S+CE G   P +W   +
Sbjct: 164 KTFASWGVDYLKYDNCENLGISVKERYPPMGKALLSSGRPIFFSMCEWGWEDPQIWAKSI 223

Query: 213 ANSWRTTSDIEDNWDR----ADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSI 272
            NSWRTT DIEDNW+     AD ND +A YA PGGWNDPDMLEVGNGGMT EEYR HFSI
Sbjct: 224 GNSWRTTGDIEDNWNSMTSIADSNDKWASYAGPGGWNDPDMLEVGNGGMTTEEYRSHFSI 283

Query: 273 WAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVRS-GHVEVWAGPLSG 315
           WA++KAPLL+G D+R + +   E+I N+EVI+VNQD LGVQ KKV+S   +EVWAGPLS 
Sbjct: 284 WALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDKLGVQGKKVKSTNDLEVWAGPLSD 343

BLAST of CmoCh18G008480 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.0e-98
Identity = 188/349 (53.87%), Postives = 226/349 (64.76%), Query Frame = 0

Query: 4   LAAAIASILLSFLLIPPSAAASRKLR---PQDRRNLLANALASTPPMGWNSWNHFRCGIS 63
           L A  A++L     +    A SR  R    + RR    N L  TP MGWNSWNHF CGI+
Sbjct: 20  LVAVAATLLPEAAALGNFTAESRGARWRSRRARRRAFENGLGRTPQMGWNSWNHFYCGIN 79

Query: 64  ETIVKQNAHLLVSTGLSKLGYVYVNI------------------------------DYVH 123
           E I+++ A  LV+TGL+KLGY YVNI                              DYVH
Sbjct: 80  EQIIRETADALVNTGLAKLGYQYVNIDDCWAEYSRDSQGNFVPNRQTFPSGIKALADYVH 139

Query: 124 NKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDAKTFANWGVDYLKYDNCNNDNIKPTERYP 183
            KGLKLGIYSD+G+ TCS  MPGSL HEEQD KTFA+WGVDYLKYDNCN+      ERY 
Sbjct: 140 AKGLKLGIYSDAGSQTCSNKMPGSLDHEEQDVKTFASWGVDYLKYDNCNDAGRSVMERYT 199

Query: 184 IMTRALMKAGRPIFLSLCEGGDMHPAMWGADVANSWRTTSDIEDNW----DRADLNDAFA 243
            M+ A+   G+ IF SLCE G  +PA W   + NSWRTT DI DNW     RAD ND +A
Sbjct: 200 RMSNAMKTYGKNIFFSLCEWGKENPATWAGRMGNSWRTTGDIADNWGSMTSRADENDQWA 259

Query: 244 EYARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISEDPMEIIGNS 303
            YA PGGWNDPDMLEVGNGGM++ EYR HFSIWA++KAPLL+G D+R +S+    I+ NS
Sbjct: 260 AYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSIWALAKAPLLIGCDVRSMSQQTKNILSNS 319

Query: 304 EVISVNQDPLGVQAKKVRSGH-VEVWAGPLSGYRVAVLLVNRRKWRMAV 315
           EVI+VNQD LGVQ KKV+S + +EVWAGPLS  R AV+L NR+ ++  +
Sbjct: 320 EVIAVNQDSLGVQGKKVQSDNGLEVWAGPLSNNRKAVVLWNRQSYQATI 368

BLAST of CmoCh18G008480 vs. ExPASy TrEMBL
Match: A0A6J1G1S0 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449899 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 6.0e-162
Identity = 291/323 (90.09%), Postives = 294/323 (91.02%), Query Frame = 0

Query: 1   METLAAAIASILLSFLLIPPSAAASRKLRPQDRRNLLANALASTPPMGWNSWNHFRCGIS 60
           METLAAAIASILLSFLLIPPSAAASRKLRPQDRRNLLANALASTPPMGWNSWNHFRCGIS
Sbjct: 1   METLAAAIASILLSFLLIPPSAAASRKLRPQDRRNLLANALASTPPMGWNSWNHFRCGIS 60

Query: 61  ETIVKQNAHLLVSTGLSKLGYVYVNI------DYVHNKGLKLGIYSDSGTFTCSKTMPGS 120
           ETIVKQNAHLLVSTGLSKLGYVYVNI      DYVHNKGLKLGIYSDSGTFTCSKTMPGS
Sbjct: 61  ETIVKQNAHLLVSTGLSKLGYVYVNIGIKSLADYVHNKGLKLGIYSDSGTFTCSKTMPGS 120

Query: 121 LYHEEQDAKTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMH 180
           LYHEEQDAKTFANWGVDYLKYDNCNNDNIKPTER+                +LC  GDMH
Sbjct: 121 LYHEEQDAKTFANWGVDYLKYDNCNNDNIKPTERW----------------ALC--GDMH 180

Query: 181 PAMWGADVANSWRTTSDIEDNWDRADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRV 240
           PAMWGADVANSWRTTSDIEDNWDRADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRV
Sbjct: 181 PAMWGADVANSWRTTSDIEDNWDRADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRV 240

Query: 241 HFSIWAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVRSGH----VEV 300
           HFSIWAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVRSGH     +V
Sbjct: 241 HFSIWAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVRSGHEKKMEQV 300

Query: 301 WAGPLSGYRVAVLLVNRRKWRMA 314
           WAGPLSGYRVAVLLVNRRKWRMA
Sbjct: 301 WAGPLSGYRVAVLLVNRRKWRMA 305

BLAST of CmoCh18G008480 vs. ExPASy TrEMBL
Match: A0A540MM48 (Pyruvate kinase OS=Malus baccata OX=106549 GN=C1H46_014596 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.7e-127
Identity = 231/329 (70.21%), Postives = 253/329 (76.90%), Query Frame = 0

Query: 21  SAAASRKLRPQDRRNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLG 80
           S+AAS     Q RRNLLAN L  TPPMGWNSWNHF C I E I+K  A  LVSTGLSKLG
Sbjct: 551 SSAASAS--SQLRRNLLANGLGLTPPMGWNSWNHFNCKIDEKIIKATADALVSTGLSKLG 610

Query: 81  YVYVNI------------------------------DYVHNKGLKLGIYSDSGTFTCSKT 140
           Y YVNI                              DYVH+KGLKLGIYSD+G FTCSKT
Sbjct: 611 YTYVNIDDCWAEIARDHKRNLVPKKSTFPSGIKALADYVHSKGLKLGIYSDAGYFTCSKT 670

Query: 141 MPGSLYHEEQDAKTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEG 200
           MPGSL HEEQDAKTFA WG+DYLKYDNCNND+ KPT RYP+MTRALMKAGRPIF SLCE 
Sbjct: 671 MPGSLGHEEQDAKTFAAWGIDYLKYDNCNNDDSKPTVRYPVMTRALMKAGRPIFFSLCEW 730

Query: 201 GDMHPAMWGADVANSWRTTSDIEDNWD----RADLNDAFAEYARPGGWNDPDMLEVGNGG 260
           GD+HPA WGA V NSWRTT+DI D W+    RAD+N+ +AE+ARPGGWNDPDMLEVGNGG
Sbjct: 731 GDLHPAQWGAKVGNSWRTTNDISDTWESMISRADMNEVYAEFARPGGWNDPDMLEVGNGG 790

Query: 261 MTKEEYRVHFSIWAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVRS- 315
           MTK+EY VHFSIWAISKAPLLLG D+R I+++ MEII N EVISVNQDPLGVQAKKVRS 
Sbjct: 791 MTKDEYVVHFSIWAISKAPLLLGCDVRNITKENMEIIANKEVISVNQDPLGVQAKKVRSE 850

BLAST of CmoCh18G008480 vs. ExPASy TrEMBL
Match: A0A5N5L969 (Alpha-galactosidase OS=Salix brachista OX=2182728 GN=DKX38_016735 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.7e-127
Identity = 222/321 (69.16%), Postives = 248/321 (77.26%), Query Frame = 0

Query: 29  RPQDRRNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI-- 88
           R   RRNLLAN LA+TPPMGWNSWNHF C I E I+K+ A  LVSTGLSKLGY YVNI  
Sbjct: 57  RDSSRRNLLANGLAATPPMGWNSWNHFNCKIDEKIIKETADALVSTGLSKLGYTYVNIDD 116

Query: 89  ----------------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHE 148
                                       DYVHNKGLKLGIYSD+G FTCSKTMPGSL HE
Sbjct: 117 CWAEIARDGKGNLVPKKSTFPSGIKALADYVHNKGLKLGIYSDAGYFTCSKTMPGSLGHE 176

Query: 149 EQDAKTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMW 208
           EQDAK+FA+WG+DYLKYDNCNND  KPT RYP+MTRALMKAGRPIF SLCE GDMHPA W
Sbjct: 177 EQDAKSFASWGIDYLKYDNCNNDGTKPTVRYPVMTRALMKAGRPIFHSLCEWGDMHPATW 236

Query: 209 GADVANSWRTTSDIEDNWD----RADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRV 268
           GA V NSWRTT DI D WD    RAD+N+ +AE+ARPGGWNDPDMLEVGNGGMTK+EY V
Sbjct: 237 GAKVGNSWRTTGDISDTWDSMVSRADMNEVYAEFARPGGWNDPDMLEVGNGGMTKDEYTV 296

Query: 269 HFSIWAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVR-SGHVEVWAG 315
           HFSIWAISKAPLLLG D+R ++++ M+I+ N EVI+VNQD LGVQAKKVR  G +E+WAG
Sbjct: 297 HFSIWAISKAPLLLGCDVRNMTKETMDIVANKEVIAVNQDSLGVQAKKVRMEGDLEIWAG 356

BLAST of CmoCh18G008480 vs. ExPASy TrEMBL
Match: A0A6N2LC77 (Alpha-galactosidase OS=Salix viminalis OX=40686 GN=SVIM_LOCUS208977 PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 4.8e-127
Identity = 223/321 (69.47%), Postives = 248/321 (77.26%), Query Frame = 0

Query: 29  RPQDRRNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI-- 88
           R   RRNLLAN LA+TPPMGWNSWNHF C I E I+K+ A  LVSTGLSKLGY YVNI  
Sbjct: 58  RDSSRRNLLANGLAATPPMGWNSWNHFNCKIDEKIIKETADALVSTGLSKLGYTYVNIDD 117

Query: 89  ----------------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHE 148
                                       DYVHNKGLKLGIYSD+G FTCSKTMPGSL HE
Sbjct: 118 CWAEIARDGKGNLVPKKSTFPSGIKALADYVHNKGLKLGIYSDAGYFTCSKTMPGSLGHE 177

Query: 149 EQDAKTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMW 208
           EQDAK+FA+WG+DYLKYDNCNND  KPT RYP+MTRALMKAGRPIF SLCE GDMHPA W
Sbjct: 178 EQDAKSFASWGIDYLKYDNCNNDGTKPTVRYPVMTRALMKAGRPIFHSLCEWGDMHPATW 237

Query: 209 GADVANSWRTTSDIEDNWD----RADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRV 268
           GA V NSWRTT DI D WD    RAD+N+ +AE ARPGGWNDPDMLEVGNGGMTK+EY V
Sbjct: 238 GAKVGNSWRTTGDISDTWDSMVSRADMNEVYAELARPGGWNDPDMLEVGNGGMTKDEYTV 297

Query: 269 HFSIWAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVR-SGHVEVWAG 315
           HFSIWAISKAPLLLG D+R ++++ M+II N EVI+VNQD LGVQAKKVR  G +E+WAG
Sbjct: 298 HFSIWAISKAPLLLGCDVRNMTKETMDIIANKEVIAVNQDSLGVQAKKVRMEGDLEIWAG 357

BLAST of CmoCh18G008480 vs. ExPASy TrEMBL
Match: A0A4U5R207 (Alpha-galactosidase OS=Populus alba OX=43335 GN=D5086_0000020620 PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.6e-125
Identity = 220/317 (69.40%), Postives = 244/317 (76.97%), Query Frame = 0

Query: 33  RRNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI------ 92
           RRNLLAN LA TPPMGWNSWNHF C I E I+K  A  LVSTGLSKLGY YVNI      
Sbjct: 58  RRNLLANGLADTPPMGWNSWNHFNCKIDEKIIKATADFLVSTGLSKLGYTYVNIDDCWAE 117

Query: 93  ------------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDA 152
                                   DYVH+KGLKLGIYSD+G FTCSKTMPGSL HEEQDA
Sbjct: 118 MARDGKGNLVPKKSTFPSGIKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDA 177

Query: 153 KTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADV 212
           K+FA+WG+DYLKYDNCNND  KPT RYP+MTRALMK G PIF SLCE GDMHPA WGA V
Sbjct: 178 KSFASWGIDYLKYDNCNNDGTKPTVRYPVMTRALMKTGHPIFFSLCEWGDMHPATWGAKV 237

Query: 213 ANSWRTTSDIEDNWD----RADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSI 272
            NSWRTT+DI D WD    RAD+N+ +AE ARPGGWNDPDMLEVGNGGMTK+EY VHFSI
Sbjct: 238 GNSWRTTNDISDTWDSMVSRADMNEVYAELARPGGWNDPDMLEVGNGGMTKDEYIVHFSI 297

Query: 273 WAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVR-SGHVEVWAGPLSG 315
           WAISKAPLLLG D+R ++++ M+II N EVI+VNQDPLGVQAKKVR  G +E+WAGPL+G
Sbjct: 298 WAISKAPLLLGCDVRNMTKETMDIIANKEVIAVNQDPLGVQAKKVRMEGDLEIWAGPLAG 357

BLAST of CmoCh18G008480 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 434.9 bits (1117), Expect = 6.0e-122
Identity = 212/336 (63.10%), Postives = 249/336 (74.11%), Query Frame = 0

Query: 33  RRNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI------ 92
           RR+LL N L  TPPMGWNSWNHF C I E ++K+ A  LV+TGLSKLGY YVNI      
Sbjct: 42  RRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNIDDCWAE 101

Query: 93  ------------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDA 152
                                   DYVH+KGLKLGIYSD+G FTCSKTMPGSL +EE DA
Sbjct: 102 ISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDA 161

Query: 153 KTFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADV 212
           KTFA WG+DYLKYDNCN+D  KPT RYP+MTRALMK+GRPIF SLCE GDMHPA+WG+ V
Sbjct: 162 KTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPALWGSPV 221

Query: 213 ANSWRTTSDIEDNW----DRADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSI 272
            NSWRTT+DI+D W      AD+N+ +AE+ARPGGWNDPDMLEVGNGGMTK+EY VHFSI
Sbjct: 222 GNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYIVHFSI 281

Query: 273 WAISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVR-SGHVEVWAGPLSG 332
           WAISKAPLLLG D+R ++++ MEI+ N EVI++NQDP GVQAKKVR  G +EVWAGPLSG
Sbjct: 282 WAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWAGPLSG 341

Query: 333 YRVAVLLVNRRKWRMAVLSGELSRTLRYPFVEVVRA 334
           YRVA+LL+NR   R ++ +  L   +  P   +V A
Sbjct: 342 YRVALLLLNRGPSRTSITA--LWEDIEIPANSIVEA 375

BLAST of CmoCh18G008480 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 387.1 bits (993), Expect = 1.4e-107
Identity = 185/309 (59.87%), Postives = 219/309 (70.87%), Query Frame = 0

Query: 34  RNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI------- 93
           R L+ N LA +P MGWNSWNHF+C I+ET++KQ A  +VS+GLS +GY Y+NI       
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 88

Query: 94  -----------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDAK 153
                                  DYVH+KGLKLGIYSD+GT TCS+TMPGSL HEEQDAK
Sbjct: 89  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 148

Query: 154 TFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADVA 213
           TFA+WG+DYLKYDNC N    P ERYP M++AL+ +GR IF SLCE G   PA W  D+ 
Sbjct: 149 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 208

Query: 214 NSWRTTSDIEDNWDR----ADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSIW 273
           NSWRTT DI+DNW      AD ND +A YARPG WNDPDMLEVGNGGMTKEEY  HFSIW
Sbjct: 209 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 268

Query: 274 AISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKV-RSGHVEVWAGPLSGY 308
           A++KAPLL+G DLR + +   E++ N EVI+VNQD LG+Q KKV + G +EVWAGPLS  
Sbjct: 269 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 328

BLAST of CmoCh18G008480 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 387.1 bits (993), Expect = 1.4e-107
Identity = 185/309 (59.87%), Postives = 219/309 (70.87%), Query Frame = 0

Query: 34  RNLLANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI------- 93
           R L+ N LA +P MGWNSWNHF+C I+ET++KQ A  +VS+GLS +GY Y+NI       
Sbjct: 3   RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 62

Query: 94  -----------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDAK 153
                                  DYVH+KGLKLGIYSD+GT TCS+TMPGSL HEEQDAK
Sbjct: 63  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 122

Query: 154 TFANWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADVA 213
           TFA+WG+DYLKYDNC N    P ERYP M++AL+ +GR IF SLCE G   PA W  D+ 
Sbjct: 123 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 182

Query: 214 NSWRTTSDIEDNWDR----ADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSIW 273
           NSWRTT DI+DNW      AD ND +A YARPG WNDPDMLEVGNGGMTKEEY  HFSIW
Sbjct: 183 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 242

Query: 274 AISKAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKV-RSGHVEVWAGPLSGY 308
           A++KAPLL+G DLR + +   E++ N EVI+VNQD LG+Q KKV + G +EVWAGPLS  
Sbjct: 243 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 302

BLAST of CmoCh18G008480 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.9e-99
Identity = 178/310 (57.42%), Postives = 216/310 (69.68%), Query Frame = 0

Query: 37  LANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI------DYVH 96
           L N LA TP MGWNSWN F C I+ET++K+ A  LVS+GL+ LGY++VNI      DYVH
Sbjct: 65  LNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIGIKLLADYVH 124

Query: 97  NKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDAKTFANWGVDYLKYDNCNNDNIKPTERYP 156
           +KGLKLGIYSD+G FTC +  PGSL+HE  DA  FA+WGVDYLKYDNC N  IKP ERYP
Sbjct: 125 SKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFASWGVDYLKYDNCFNLGIKPIERYP 184

Query: 157 IMTRALMKAGRPIFLSLCEGGDMHPAMWGADVANSWRTTSDIEDNWDR----ADLNDAFA 216
            M  AL   GR IF SLCE G   PA+W  +V NSWRTT DI D W      ADLN+ +A
Sbjct: 185 PMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWA 244

Query: 217 EYARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISEDPMEIIGNS 276
            YA PGGWNDPDMLE+GNGGMT EEYR HFSIWA+ KAPLL+G D+R ++ + +EI+ N 
Sbjct: 245 AYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNK 304

Query: 277 EVISVNQDPLGVQAKKVRSGH----VEVWAGPLSGYRVAVLLVNR--------RKWRMAV 325
           E+I+VNQDPLGVQ +K+++       +VW+GPLSG R+ V L NR          W M  
Sbjct: 305 EIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIG 364

BLAST of CmoCh18G008480 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.1e-96
Identity = 178/334 (53.29%), Postives = 216/334 (64.67%), Query Frame = 0

Query: 37  LANALASTPPMGWNSWNHFRCGISETIVKQNAHLLVSTGLSKLGYVYVNI---------- 96
           L N LA TP MGWNSWN F C I+ET++K+ A  LVS+GL+ LGY++VNI          
Sbjct: 65  LNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIDDCWSNLLRD 124

Query: 97  --------------------DYVHNKGLKLGIYSDSGTFTCSKTMPGSLYHEEQDAKTFA 156
                               DYVH+KGLKLGIYSD+G FTC +  PGSL+HE  DA  FA
Sbjct: 125 SEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFA 184

Query: 157 NWGVDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEGGDMHPAMWGADVANSW 216
           +WGVDYLKYDNC N  IKP ERYP M  AL   GR IF SLCE G   PA+W  +V NSW
Sbjct: 185 SWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSW 244

Query: 217 RTTSDIEDNWDR----ADLNDAFAEYARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAIS 276
           RTT DI D W      ADLN+ +A YA PGGWNDPDMLE+GNGGMT EEYR HFSIWA+ 
Sbjct: 245 RTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALM 304

Query: 277 KAPLLLGNDLREISEDPMEIIGNSEVISVNQDPLGVQAKKVRSGH----VEVWAGPLSGY 325
           KAPLL+G D+R ++ + +EI+ N E+I+VNQDPLGVQ +K+++       +VW+GPLSG 
Sbjct: 305 KAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGD 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FT978.5e-12163.10Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1[more]
Q426568.8e-11060.97Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1[more]
Q8RX862.0e-10659.87Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1[more]
P147499.4e-10458.36Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1[more]
Q9FXT42.0e-9853.87Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1G1S06.0e-16290.09Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449899 PE=3 SV=1[more]
A0A540MM481.7e-12770.21Pyruvate kinase OS=Malus baccata OX=106549 GN=C1H46_014596 PE=3 SV=1[more]
A0A5N5L9693.7e-12769.16Alpha-galactosidase OS=Salix brachista OX=2182728 GN=DKX38_016735 PE=3 SV=1[more]
A0A6N2LC774.8e-12769.47Alpha-galactosidase OS=Salix viminalis OX=40686 GN=SVIM_LOCUS208977 PE=3 SV=1[more]
A0A4U5R2072.6e-12569.40Alpha-galactosidase OS=Populus alba OX=43335 GN=D5086_0000020620 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08380.16.0e-12263.10alpha-galactosidase 1 [more]
AT5G08370.11.4e-10759.87alpha-galactosidase 2 [more]
AT5G08370.21.4e-10759.87alpha-galactosidase 2 [more]
AT3G56310.21.9e-9957.42Melibiase family protein [more]
AT3G56310.11.1e-9653.29Melibiase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002241Glycoside hydrolase, family 27PRINTSPR00740GLHYDRLASE27coord: 75..90
score: 47.19
coord: 39..58
score: 58.5
IPR002241Glycoside hydrolase, family 27PFAMPF16499Melibiase_2coord: 87..274
e-value: 8.7E-50
score: 169.5
IPR002241Glycoside hydrolase, family 27PANTHERPTHR11452ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASEcoord: 31..87
coord: 87..312
IPR002241Glycoside hydrolase, family 27CDDcd14792GH27coord: 45..274
e-value: 6.77341E-124
score: 354.939
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 277..322
e-value: 1.2E-5
score: 27.3
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 37..276
e-value: 6.8E-101
score: 339.4
NoneNo IPR availablePANTHERPTHR11452:SF82ALPHA-GALACTOSIDASEcoord: 31..87
coord: 87..312
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 276..312
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 39..275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh18G008480.1CmoCh18G008480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0019752 carboxylic acid metabolic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0046872 metal ion binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0052692 raffinose alpha-galactosidase activity
molecular_function GO:0016772 transferase activity, transferring phosphorus-containing groups
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds