CmoCh18G005030 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh18G005030 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 1.1e-149
Identity = 267/532 (50.19%), Postives = 368/532 (69.17%), Query Frame = 0

Query: 9   SPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSYSSV 68
           S +L    T  +L S    VS+N      +FLQCLS    D + +S V++TP ++S+SSV
Sbjct: 8   SSVLIFFTTSTLLLSIPHPVSAN-RSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 67

Query: 69  LNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA 128
           L  +++N RFS P  PKP+LI+TP+  S +Q+AV C++  G+ IRTRSGGHD+EGLSYV 
Sbjct: 68  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 127

Query: 129 SLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGG 188
             PF+I+DL NL SI++DV+N++ WVQ+GAT+GELYY I KK++TLAFPAG+CPTVGVGG
Sbjct: 128 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 187

Query: 189 HFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFGIVVA 248
           HFSGGGYG LLRK+GLAAD+VIDA++VDA G+ + R+ MGED FWAIRGGGG SF +V++
Sbjct: 188 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 247

Query: 249 WKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGK 308
           WK+ L+ VP++VT+    K SE  A K+IH+WQ+VA K+ ++ F+ ++L       Q  K
Sbjct: 248 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYK 307

Query: 309 INPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGSKLED 368
               A F  LYLG    LL ++N  FPELGL + DC E SW+E+ +      +LG +  +
Sbjct: 308 NMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPIN 367

Query: 369 LLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEF 428
           +L+K T  + + K KSD+++EP+P  AI  +W RL+  + E  Q IF P+GGKM +I ++
Sbjct: 368 VLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADY 427

Query: 429 ETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDL 488
           ETPF HR  N+Y+I Y+  W     + +++++ W+  +Y  M+ FV+KSPR AY+N RDL
Sbjct: 428 ETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDL 487

Query: 489 DIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 541
           D+G      R+ YE+   WG KYF K NF+RLV VKT VDP DFF  EQSIP
Sbjct: 488 DLGMYVGVKRSKYEEGKSWGVKYF-KNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CmoCh18G005030 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 9.1e-149
Identity = 262/521 (50.29%), Postives = 354/521 (67.95%), Query Frame = 0

Query: 28  VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSYSSVLNITLRNLRFSTPHTPKPL 87
           + ++   P +NFL+C S Y  + ++   ++YT  N  Y SVLN T+ NLRF++  TPKPL
Sbjct: 23  IQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIVDLINLNSISIDV 147
           +I+TP HVSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N+ SI IDV
Sbjct: 83  VIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDV 142

Query: 148 ENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAAD 207
            ++TAWV++GATLGE+YY + +K++ L+  AG CPTV  GGHF GGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANK 267
           N+IDA LV+ +GK ++R+ MGEDLFWA+RGGG  SFGI+VAWK++LV VP S    +   
Sbjct: 203 NIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKSTMFSVKKI 262

Query: 268 TSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPLAL---FFSLYLGKAN 327
                  KL+++WQ +A+K + +  L       N T   GK N  A+   F S++LG  +
Sbjct: 263 MEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGK-NKTAIHTYFSSVFLGGVD 322

Query: 328 ELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS---KLEDLLSKATLVNGSTK 387
            L+ ++N +FPELG+ K DC + SW++T +     +   +     E LL ++   NG+ K
Sbjct: 323 SLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFK 382

Query: 388 IKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGENLYK 447
           IK DY+K+PIP +    I E+L  EDI    +   PYGG M +I E   PF HR   LY+
Sbjct: 383 IKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYE 442

Query: 448 IGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTSY 507
           + YI +W +Q  E  ++H++WIR +Y++MTP+VSK+PR AYLNYRDLDIG N+     +Y
Sbjct: 443 LWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNY 502

Query: 508 EQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
            QA IWG KYFGK NFDRLV VKT VDP +FFR+EQSIP L
Sbjct: 503 TQARIWGEKYFGK-NFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of CmoCh18G005030 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.2e-148
Identity = 260/521 (49.90%), Postives = 356/521 (68.33%), Query Frame = 0

Query: 28  VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSYSSVLNITLRNLRFSTPHTPKPL 87
           +  +   P +NFL+C S Y     + + ++YT  +  Y S+LN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIVDLINLNSISIDV 147
           +IITPL+VSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N++S+ IDV
Sbjct: 83  VIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDV 142

Query: 148 ENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAAD 207
            ++TAWV++GATLGE+YY I + ++ L+FPAG CPTVG GGHFSGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANK 267
           N+IDA LV+ +GK ++R+ MGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T+    K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKK 262

Query: 268 TSE-DGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPL--ALFFSLYLGKAN 327
             E     KL+++WQ +A+  E    L       N T   GK      + F S++ G  +
Sbjct: 263 NMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVD 322

Query: 328 ELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS---KLEDLLSKATLVNGSTK 387
            L+ ++N +FPELG+ K DC + SW++T +     +   +   K E LL ++     +  
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFS 382

Query: 388 IKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGENLYK 447
           IK DY+K+PIP  A+  I E+L  ED+ +   +F PYGG M +I E   PF HR   +Y+
Sbjct: 383 IKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYE 442

Query: 448 IGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTSY 507
           I YI +W +Q  E  ++HI+WIR +Y++ TP+VS++PR AYLNYRDLD+G  N     +Y
Sbjct: 443 IWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNY 502

Query: 508 EQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
            QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 503 TQARIWGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CmoCh18G005030 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 261/521 (50.10%), Postives = 355/521 (68.14%), Query Frame = 0

Query: 28  VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSYSSVLNITLRNLRFSTPHTPKPL 87
           +  +   P +NFL+CLS Y     + + ++YT  +  Y S+LN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIVDLINLNSISIDV 147
           +I TPL+VSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N++S+ IDV
Sbjct: 83  VITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDV 142

Query: 148 ENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAAD 207
            ++TAWV+SGATLGE+YY I + ++ L+FPAG CPTVG GGHFSGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANK 267
           N+IDA LV+ +GK ++R+ MGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T+    K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKK 262

Query: 268 TSE-DGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPL--ALFFSLYLGKAN 327
             E     KL+++WQ +A+  E    L       N T   GK      + F S++ G  +
Sbjct: 263 NMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVD 322

Query: 328 ELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS---KLEDLLSKATLVNGSTK 387
            L+ ++N +FPELG+ K DC + SW++T +     +   +   K E LL ++     +  
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFS 382

Query: 388 IKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGENLYK 447
           IK DY+K+PIP  A+  I E+L  ED+ +   +F PYGG M +I E   PF HR    Y+
Sbjct: 383 IKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYE 442

Query: 448 IGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTSY 507
           I YI +W +Q  E  ++HI+WIR +Y++ TP+VS++PR AYLNYRDLD+G  N     +Y
Sbjct: 443 IWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNY 502

Query: 508 EQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
            QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 503 TQARIWGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CmoCh18G005030 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 5.0e-147
Identity = 254/521 (48.75%), Postives = 352/521 (67.56%), Query Frame = 0

Query: 28  VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSYSSVLNITLRNLRFSTPHTPKPL 87
           +  +   P +NFL+C S Y  +  +    +YT  +  Y SVLN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIVDLINLNSISIDV 147
           +I+TP +VSHIQA++LCSK  GLQIRTRSGGHD EGLSY++ +PF IVDL N++++ +D+
Sbjct: 83  VIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDI 142

Query: 148 ENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAAD 207
            ++TAWV++GATLGE+YY I + ++  +FP G CPTVGVGGHFSGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANK 267
           N+IDA LV+ +GK ++R+ MGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T+    K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKK 262

Query: 268 TSE-DGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPL--ALFFSLYLGKAN 327
             E  G  KL ++WQ +A+K + +  L       N T   GK        F S++LG  +
Sbjct: 263 NMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVD 322

Query: 328 ELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS---KLEDLLSKATLVNGSTK 387
            L+ ++N +FPELG+ K DC E SW++T +     +   +   K E LL ++     +  
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFS 382

Query: 388 IKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGENLYK 447
           IK DY+K+ IP  A+  I E+L  E++ +  ++  PYGG M +I E   PF HR   +Y+
Sbjct: 383 IKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYE 442

Query: 448 IGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTSY 507
           + Y   W +Q  E  ++HI+W+R +Y++ TP+VS++PR AYLNYRDLD+G  N     +Y
Sbjct: 443 LWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNY 502

Query: 508 EQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
            QA IWG KYFGK NF+RLV VKTK DP +FFR+EQSIP L
Sbjct: 503 TQARIWGEKYFGK-NFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of CmoCh18G005030 vs. ExPASy TrEMBL
Match: A0A6J1GRE1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456783 PE=3 SV=1)

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 546
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005030 vs. ExPASy TrEMBL
Match: A0A6J1K2F5 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490442 PE=3 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 538/545 (98.72%), Postives = 542/545 (99.45%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVE+KTAWVQSGATLGELYY IAKKS+TLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNR+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKA+ELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           QLGSKLEDLLSKA LVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGE LYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 546
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005030 vs. ExPASy TrEMBL
Match: A0A6J1K3Z1 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490441 PE=3 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 6.0e-297
Identity = 505/549 (91.99%), Postives = 524/549 (95.45%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLI LNSISIDVE+KTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLIKLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYG LLRKYGLAADNVIDAQLVDANGKFVNR+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRELMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKV LV VPASVTLCMA+KTSEDGATKLIHQWQYVAHKLEN+ FLGILL GG
Sbjct: 241 GSFGIVVAWKVNLVSVPASVTLCMASKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGT-- 360
           NF+RQG KINP+ALFFSLYLGKA+EL+ IL+T FPELGLSKKDCMETSWVETA+IMGT  
Sbjct: 301 NFSRQGDKINPVALFFSLYLGKADELVAILDTKFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 --GLQLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 420
             GLQ GSKLEDL SK T +N STKIKSDY+KEPIPMAAIEGIWERLR +DIE+PQH+FV
Sbjct: 361 QPGLQPGSKLEDLQSKTTPINASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQHLFV 420

Query: 421 PYGGKMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 480
           PYGG+MGQI EFETPFSHRGE +YKIGY V W E GI+AEKRHIDWIRE+YSYMTPFVSK
Sbjct: 421 PYGGRMGQIQEFETPFSHRGEYMYKIGYTVGWVEGGIDAEKRHIDWIREIYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 546
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 549

BLAST of CmoCh18G005030 vs. ExPASy TrEMBL
Match: A0A6J1GRG1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456798 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 3.9e-296
Identity = 503/549 (91.62%), Postives = 524/549 (95.45%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQDGSSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDV++KTAWVQSGATLGELYYSIAKKSQTL FPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVKSKTAWVQSGATLGELYYSIAKKSQTLTFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYG LLRKYGLAADNVIDAQLVDANGKFVNR+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLV VPASVTLCM NKTSEDGATKLIHQWQYVAHKLEN+ FLGILL GG
Sbjct: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360
           NFTRQG KINP+ALFFSLYLGKA+EL+ ILNTTFPELGLSKKDCMETSWVETA+IMGTGL
Sbjct: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 QL----GSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 420
           QL    GSKLEDLLSK T +  STKIKSDY+KEPIPMAAIEGIWERLR +DIE+PQ+IFV
Sbjct: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420

Query: 421 PYGGKMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 480
           PYGG+MGQI E ETPFSHRGE +YKIGY+V W EQGI+AEKRHIDWIRELYSYMTPFVSK
Sbjct: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFG ENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGNENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 546
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 549

BLAST of CmoCh18G005030 vs. ExPASy TrEMBL
Match: A0A6J1GTA5 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456788 PE=3 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 2.2e-283
Identity = 480/545 (88.07%), Postives = 503/545 (92.29%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSSP LP SLT LV CSCSSWVSSNF KPH+NFLQCLSHYSQD SSIS+VLYTP
Sbjct: 1   MSNSAMNSSPFLPFSLTFLVFCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
            EGLSYVASLPFIIVDLINLNSISIDVE+KTAWVQSGATLGELYY IA+KS++LAFPAGI
Sbjct: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGGYG+LLRKYGLAADNVIDAQLVDANGKF +R+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKV LV VPA+VTLC+ NKT EDGATKLIHQWQYVAHKLENN FL ILL GG
Sbjct: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLSILLEGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360
           NFTRQG KINPLALFFSLYLGKA+ELLTILNTTFPELGLSK DCMETSWVETALIMGTGL
Sbjct: 301 NFTRQGSKINPLALFFSLYLGKADELLTILNTTFPELGLSKTDCMETSWVETALIMGTGL 360

Query: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           Q GSKLEDLLSK  L+   TK+KSDY+KEPIPMAAIEGIW+RLR  DIE PQ IF+PYGG
Sbjct: 361 QPGSKLEDLLSKTPLMTVRTKMKSDYVKEPIPMAAIEGIWQRLRSGDIETPQIIFIPYGG 420

Query: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KM +I E ETPFSHRGE  YKIGY  NW E GI+AE +H++WIRELYSYM PFVSKSPRA
Sbjct: 421 KMSEISELETPFSHRGEYSYKIGYTTNWIEGGIDAENKHLNWIRELYSYMAPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRT+YEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTNYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 546
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005030 vs. NCBI nr
Match: XP_022954543.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 546
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005030 vs. NCBI nr
Match: XP_022994835.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 538/545 (98.72%), Postives = 542/545 (99.45%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVE+KTAWVQSGATLGELYY IAKKS+TLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNR+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKA+ELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           QLGSKLEDLLSKA LVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGE LYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 546
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005030 vs. NCBI nr
Match: KAG6573426.1 (Cannabidiolic acid synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 536/545 (98.35%), Postives = 542/545 (99.45%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVE+KTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFV+R+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKA+ELLTILNTTFPELGLSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360

Query: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           Q GSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGE LYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 546
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005030 vs. NCBI nr
Match: XP_023542525.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 537/546 (98.35%), Postives = 542/546 (99.27%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVS+NFVKPHQ+FLQCLSHYSQD SSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSANFVKPHQSFLQCLSHYSQDASSISDVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVE+KTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNR-QLMGEDLFWAIRGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNR +LMGEDLFWAIRGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKKLMGEDLFWAIRGGG 240

Query: 241 GGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAG 300
           GGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAG
Sbjct: 241 GGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAG 300

Query: 301 GNFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTG 360
           GNFTRQGGKINPLALFFSLYLGKA+ELLTILNTTFPELGLSKKDCMETSWVETALIMGTG
Sbjct: 301 GNFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTG 360

Query: 361 LQLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYG 420
           LQ GSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYG
Sbjct: 361 LQFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYG 420

Query: 421 GKMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPR 480
           GKMGQIPEFETPFSHRGE LYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPR
Sbjct: 421 GKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPR 480

Query: 481 AAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSI 540
           AAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSI
Sbjct: 481 AAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSI 540

Query: 541 PTLSSK 546
           PTLSSK
Sbjct: 541 PTLSSK 546

BLAST of CmoCh18G005030 vs. NCBI nr
Match: XP_023542145.1 (LOW QUALITY PROTEIN: berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1036.6 bits (2679), Expect = 7.8e-299
Identity = 506/545 (92.84%), Postives = 525/545 (96.33%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSN AMNSSPLLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQD SSISDVLYTP
Sbjct: 1   MSNLAMNSSPLLPLSLTLLVLCSCSSWVSANFAKPHENFLQCLSHYSQDASSISDVLYTP 60

Query: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVE+KTAWVQSGATLGELYY IA+KS+TLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGG+GMLLRKYGLAADNVIDAQLVDANGKFVNR+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGFGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKT EDGATKLIHQWQYVAHKLE++ FLGIL+ GG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTLEDGATKLIHQWQYVAHKLESDVFLGILVGGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360
            F RQGGKINPLALFFSLYLGKA+ELLTILNTTFPELG+SKKDC E SWVETALIM TG 
Sbjct: 301 KFMRQGGKINPLALFFSLYLGKADELLTILNTTFPELGMSKKDCKEMSWVETALIMATGR 360

Query: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           QLGSKLEDLLSK T VNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIE+PQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKETPVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEIPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFE+P+SHRGE LYKIGY VNWAEQGI+AEKRH+DWIREL+SYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFESPYSHRGEYLYKIGYGVNWAEQGIDAEKRHMDWIRELHSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
            YLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 TYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 546
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005030 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 531.6 bits (1368), Expect = 7.6e-151
Identity = 267/532 (50.19%), Postives = 368/532 (69.17%), Query Frame = 0

Query: 9   SPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSYSSV 68
           S +L    T  +L S    VS+N      +FLQCLS    D + +S V++TP ++S+SSV
Sbjct: 8   SSVLIFFTTSTLLLSIPHPVSAN-RSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 67

Query: 69  LNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA 128
           L  +++N RFS P  PKP+LI+TP+  S +Q+AV C++  G+ IRTRSGGHD+EGLSYV 
Sbjct: 68  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 127

Query: 129 SLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGG 188
             PF+I+DL NL SI++DV+N++ WVQ+GAT+GELYY I KK++TLAFPAG+CPTVGVGG
Sbjct: 128 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 187

Query: 189 HFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFGIVVA 248
           HFSGGGYG LLRK+GLAAD+VIDA++VDA G+ + R+ MGED FWAIRGGGG SF +V++
Sbjct: 188 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 247

Query: 249 WKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGK 308
           WK+ L+ VP++VT+    K SE  A K+IH+WQ+VA K+ ++ F+ ++L       Q  K
Sbjct: 248 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYK 307

Query: 309 INPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGSKLED 368
               A F  LYLG    LL ++N  FPELGL + DC E SW+E+ +      +LG +  +
Sbjct: 308 NMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPIN 367

Query: 369 LLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEF 428
           +L+K T  + + K KSD+++EP+P  AI  +W RL+  + E  Q IF P+GGKM +I ++
Sbjct: 368 VLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADY 427

Query: 429 ETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDL 488
           ETPF HR  N+Y+I Y+  W     + +++++ W+  +Y  M+ FV+KSPR AY+N RDL
Sbjct: 428 ETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDL 487

Query: 489 DIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 541
           D+G      R+ YE+   WG KYF K NF+RLV VKT VDP DFF  EQSIP
Sbjct: 488 DLGMYVGVKRSKYEEGKSWGVKYF-KNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CmoCh18G005030 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 517.3 bits (1331), Expect = 1.5e-146
Identity = 255/515 (49.51%), Postives = 354/515 (68.74%), Query Frame = 0

Query: 36  HQNFLQCLSHYSQDGSSISDVLYTPFNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHV 95
           H++FL+CLS+   D +    V++T  +SS+ S+L+ +++N RFS   TPKP+ IITP+  
Sbjct: 28  HEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVKA 87

Query: 96  SHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA-SLPFIIVDLINLNSISIDVENKTAWV 155
           S +Q  + C++ HG+ +RTRS GH +EGLSY+A + PF ++DL NL SIS+DV+N+T WV
Sbjct: 88  SDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWV 147

Query: 156 QSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQL 215
           Q+GAT GELYY I K +++LAFPAGI PTVGVGG FSGGGYG LLRKYGLAADN+IDA +
Sbjct: 148 QTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDALV 207

Query: 216 VDANGKFVNRQLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGAT 275
           VDA+G+ ++RQ MGED FWAIRGGGG SFG++++WKVKLV VP+++T+    KTS+  A 
Sbjct: 208 VDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEAV 267

Query: 276 KLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPLALFFSLYLGKANELLTILNTTF 335
           ++I +WQY A K+ ++ F+   L   N      K    ALF  LY+G  N LL ++   F
Sbjct: 268 RIIKKWQYAADKVPDDLFIRTTLERSN------KNAVHALFTGLYIGPVNNLLALMEEKF 327

Query: 336 PELGLSKKDCMETSWVETALIMG---TGLQLGSKLEDLLSKATLVNGSTKIKSDYIKEPI 395
           PELGL K+ C E SW+E+ L       G  LG     +L+     + S K K D+++EPI
Sbjct: 328 PELGLEKEGCEEMSWIESVLWFADFPKGESLG-----VLTNRERTSLSFKGKDDFVQEPI 387

Query: 396 PMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGENLYKIGYIVNWAEQ 455
           P AAI+ IW RL   +  + + I  P+GGKM ++ E+ETPF HRG NLY+I Y+  W E+
Sbjct: 388 PEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREE 447

Query: 456 GIEAE---KRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTSYEQASIWG 515
             + +    +++ W+  +Y +MTP+VSKSPR AY+N++D+D+G      +T YE+   WG
Sbjct: 448 EDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGKSWG 507

Query: 516 YKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLS 544
            KYF K NF+RLV VKT+VDP DFF  EQSIP ++
Sbjct: 508 VKYF-KNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of CmoCh18G005030 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 515.4 bits (1326), Expect = 5.6e-146
Identity = 263/531 (49.53%), Postives = 361/531 (67.98%), Query Frame = 0

Query: 15  SLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSYSSVLNITLR 74
           +L L++      W SS+     + F QCL+  S     IS  ++   N SYSSVL   +R
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 75  NLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYV--ASLPF 134
           NLRF+T  TPKP LII   H SH+QAA+ C K H LQ++ RSGGHD++GLSYV  +  PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 135 IIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSG 194
            ++D+ NL S+ +DV +KTAWVQ+GA LGE+YY I +KS+TLA+PAGICPTVGVGGH SG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 195 GGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFGIVVAWKVK 254
           GGYG ++RKYGL  DN IDA++VD NGK ++R+LMGEDL+WAI GGGGGS+G+V+A+K+ 
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 255 LVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPL 314
           LV VP +VT+   ++T E  AT +IH+WQ VA KL +  F+  ++   N T    K    
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQK-TVR 303

Query: 315 ALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGSKLEDLLSK 374
             F +++LG    LL+ILN  FPELGL + DC ETSW+++ L   T +Q+GS    LL +
Sbjct: 304 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFW-TNIQVGSSETLLLQR 363

Query: 375 ATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPF 434
              VN   K KSDY++EPI    +E IW+++   ++E+P   F PYGG+MG+I    TPF
Sbjct: 364 NQPVN-YLKRKSDYVREPISRTGLESIWKKM--IELEIPTMAFNPYGGEMGRISSTVTPF 423

Query: 435 SHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGS 494
            +R  NL+KI Y  NW ++ +    R+++  R+LY +MTPFVSK+PR ++ NYRD+D+G 
Sbjct: 424 PYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGI 483

Query: 495 NNKYGR-TSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
           N+  G+ +SY +   +G KYF   NF+RLV +KT+VD  +FFR+EQSIP L
Sbjct: 484 NSHNGKISSYVEGKRYGKKYFA-GNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of CmoCh18G005030 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 513.1 bits (1320), Expect = 2.8e-145
Identity = 252/512 (49.22%), Postives = 352/512 (68.75%), Query Frame = 0

Query: 36  HQNFLQCLSHYSQDGSSISDVLYTPFNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHV 95
           H+NFL+CLSH   +    S +++T  + SY S+LN +++N RF    TPKP+ IITP+  
Sbjct: 34  HENFLKCLSHRINEDD--SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQA 93

Query: 96  SHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA-SLPFIIVDLINLNSISIDVENKTAWV 155
           + +Q+ + C++ HG+ IRTRSGGHD+EGLSY+A S PF+++DL NL SI++DV+N+T WV
Sbjct: 94  TDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWV 153

Query: 156 QSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQL 215
           QSGAT+GELYY I K S++LAFPAG+ PTVG+GG F GGGYG L+RKYGL+ADNVIDA +
Sbjct: 154 QSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHI 213

Query: 216 VDANGKFVNRQLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGAT 275
           VDANG F++RQ MGED FWAIRGGGG SF +V++WK++L+ VP+ VT+    KTSE  A 
Sbjct: 214 VDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAV 273

Query: 276 KLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPLALFFSLYLGKANELLTILNTTF 335
            +I++WQY+A K+ N+ F+  +L          +    A F  LYLG  ++LL ++   F
Sbjct: 274 SIINKWQYIADKVPNDLFIRAMLQ--------KETEVYASFPGLYLGPVSDLLALMKDKF 333

Query: 336 PELGLSKKDCMETSWVETALIMGTGLQLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMA 395
           PELGL   +C E SW+E+ L    G  +     ++L+K    + S K K D+I+EPIP  
Sbjct: 334 PELGLEIGNCREMSWIESVLWFIKGESM-----EILAKRKRTSRSFKGKDDFIEEPIPKT 393

Query: 396 AIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIE 455
           AI+ +W R    +  + + I  P+GGKM +I + E PF HR  NLY+I Y+  W+E+  +
Sbjct: 394 AIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDK 453

Query: 456 AE---KRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYG-RTSYEQASIWGYK 515
            +   ++++ W+  +Y +MTP+VSKSPR AY+N+RD+D+G       +T YE+A +WG K
Sbjct: 454 NKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVK 513

Query: 516 YFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
           YF K NFDRLV VKT VDP DFF  EQSIP +
Sbjct: 514 YF-KNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of CmoCh18G005030 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 504.6 bits (1298), Expect = 1.0e-142
Identity = 255/540 (47.22%), Postives = 360/540 (66.67%), Query Frame = 0

Query: 6   MNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYT-PFN-S 65
           MN++    ++L LL L    S++  +F    Q+F+ CL   S     +    +T   N S
Sbjct: 6   MNNTNAFLVTLLLLSL----SYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNAS 65

Query: 66  SYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEG 125
           S+   L  + +NLR+  P  PKP  I  PL+ +H+QAAVLC+K   L +R RSGGHD+EG
Sbjct: 66  SFKLALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEG 125

Query: 126 LSYVASL--PFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGIC 185
           LSYV+ +   F+IVDL  L  IS+D+E+ +AWV +GA++GE+YY I +KS+   FPAG+C
Sbjct: 126 LSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLC 185

Query: 186 PTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGG 245
            ++G+GGH  GG YG ++RK+GL ADNV+DA++VDA+GK +NR  MGED+FWAIRGGGGG
Sbjct: 186 TSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGG 245

Query: 246 SFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGN 305
           SFG+++AWK+KLVPVP  VT+    +T E   TKL+++WQ VA KL+ + F+ +++   +
Sbjct: 246 SFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTS 305

Query: 306 FTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQ 365
            T +  +      +   +LG AN LL ++  +FP+LGL+KKDC+ETSW+++ + +  G  
Sbjct: 306 KTPKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYI-AGFP 365

Query: 366 LGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGK 425
             +  E LL   +L     K KSDY++EPIP+  +EG+WE+L  ED   P  I+ PYGG 
Sbjct: 366 STAPSEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEED--SPLTIWNPYGGM 425

Query: 426 MGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAA 485
           M +IPE ETPF HR   L+KI ++  W + G  +E +H+ W+RE+YSYM  +VSKSPR+A
Sbjct: 426 MAKIPETETPFPHRSGTLFKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSA 485

Query: 486 YLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPT 542
           Y+NYRDLD+G N K        A  WG +YF K NF+RLV +K K DP +FFRHEQSIPT
Sbjct: 486 YVNYRDLDLGMNGK-----GSDAREWGNRYF-KGNFERLVEIKAKFDPENFFRHEQSIPT 531