CmoCh18G005010 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh18G005010 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.7e-147
Identity = 267/520 (51.35%), Postives = 354/520 (68.08%), Query Frame = 0

Query: 28  VSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSSYSDVLNFTLRNLRFSTPHTPKPL 87
           +  + A P ENFL+CLS Y     + + ++YT  +  Y  +LN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLSYVASLPFIIVDLINLNSISIDV 147
           +I TPL+VSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N++S+ IDV
Sbjct: 83  VITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDV 142

Query: 148 ESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTVGVGGHLSGGGYGLLLRKYGLAAD 207
            S+TAWV+SGATLGE+YY I E + +L+FPAG CPTVG GGH SGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFGIVVAWKVNLVSVPATVTLCVVNK 267
           N+IDA LV+ +GK  DRK MGEDLFWAIRGGGG +FGI+ AWK+ LV+VP+  T+  V K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKK 262

Query: 268 TVE-DGATKLIHQWQYVAHKLENNLFLGILLEGGNFT-RQG-SKINPLALFFSFYLGKAD 327
            +E     KL+++WQ +A+  E  L L       N T  QG +K    + F S + G  D
Sbjct: 263 NMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVD 322

Query: 328 ELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL----QPGSKLEDLLSKTPLMTVST 387
            L+ ++N +FPEL + K DC + SW++T +I  +G+        K E LL ++     + 
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKQLSWIDT-IIFYSGVVNYNTTNFKKEILLDRSGGRKAAF 382

Query: 388 KVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSEISELETPFSHRGEYSY 447
            +K DYVK+PIP  A+  I ++L   D+     +F PYGG M EISE   PF HR   +Y
Sbjct: 383 SIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITY 442

Query: 448 KIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLNYRDLDIGSNNKYGRTS 507
           +I Y  SW ++  +   KH+NWIR +Y++  P+VS++PR AYLNYRDLD+G  N     +
Sbjct: 443 EIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNN 502

Query: 508 YEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 541
           Y QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSIP
Sbjct: 503 YTQARIWGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of CmoCh18G005010 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.9e-147
Identity = 264/519 (50.87%), Postives = 351/519 (67.63%), Query Frame = 0

Query: 28  VSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSSYSDVLNFTLRNLRFSTPHTPKPL 87
           +  + A P ENFL+C S Y     + + ++YT  +  Y  +LN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLSYVASLPFIIVDLINLNSISIDV 147
           +IITPL+VSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N++S+ IDV
Sbjct: 83  VIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDV 142

Query: 148 ESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTVGVGGHLSGGGYGLLLRKYGLAAD 207
            S+TAWV++GATLGE+YY I E + +L+FPAG CPTVG GGH SGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFGIVVAWKVNLVSVPATVTLCVVNK 267
           N+IDA LV+ +GK  DRK MGEDLFWAIRGGGG +FGI+ AWK+ LV+VP+  T+  V K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKK 262

Query: 268 TVE-DGATKLIHQWQYVAHKLENNLFLGILLEGGNFT-RQG-SKINPLALFFSFYLGKAD 327
            +E     KL+++WQ +A+  E  L L       N T  QG +K    + F S + G  D
Sbjct: 263 NMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVD 322

Query: 328 ELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQPGS---KLEDLLSKTPLMTVSTK 387
            L+ ++N +FPEL + K DC + SW++T +     +   +   K E LL ++     +  
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFS 382

Query: 388 VKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSEISELETPFSHRGEYSYK 447
           +K DYVK+PIP  A+  I ++L   D+     +F PYGG M EISE   PF HR    Y+
Sbjct: 383 IKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYE 442

Query: 448 IGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLNYRDLDIGSNNKYGRTSY 507
           I Y  SW ++  +   KH+NWIR +Y++  P+VS++PR AYLNYRDLD+G  N     +Y
Sbjct: 443 IWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNY 502

Query: 508 EQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 541
            QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSIP
Sbjct: 503 TQARIWGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of CmoCh18G005010 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.5e-146
Identity = 267/535 (49.91%), Postives = 368/535 (68.79%), Query Frame = 0

Query: 9   SPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSSYSDV 68
           S +L    T  +L S    VS+N +    +FLQCLS    D++ +S V++TP ++S+S V
Sbjct: 8   SSVLIFFTTSTLLLSIPHPVSANRSN-QSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 67

Query: 69  LNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLSYVA 128
           L  +++N RFS P  PKP+LI+TP+  S +Q+AV C++  G+ IRTRSGGHD EGLSYV 
Sbjct: 68  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 127

Query: 129 SLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTVGVGG 188
             PF+I+DL NL SI++DV++++ WVQ+GAT+GELYY I +K+R+LAFPAG+CPTVGVGG
Sbjct: 128 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 187

Query: 189 HLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFGIVVA 248
           H SGGGYG LLRK+GLAAD+VIDA++VDA G+  +R+ MGED FWAIRGGGG SF +V++
Sbjct: 188 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 247

Query: 249 WKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGGNFTRQGSK 308
           WK+ L++VP+TVT+  V K  E  A K+IH+WQ+VA K+ ++LF+ ++L       Q  K
Sbjct: 248 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYK 307

Query: 309 INPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQPGSKLED 368
               A F   YLG    LL ++N  FPEL L + DC E SW+E+ +      + G +  +
Sbjct: 308 NMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPIN 367

Query: 369 LLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSEISEL 428
           +L+K    +++ K KSD+V+EP+P  AI  + +RL+  + E  Q+IF P+GGKMSEI++ 
Sbjct: 368 VLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADY 427

Query: 429 ETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLNYRDL 488
           ETPF HR    Y+I Y   W  RG D + K++ W+  +Y  M+ FV+KSPR AY+N RDL
Sbjct: 428 ETPFPHRKGNIYEIQYLNYW--RG-DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDL 487

Query: 489 DIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTFS 544
           D+G      R+ YE+   WG KYF K NF+RLV VKT VDP DFF  EQSIP F+
Sbjct: 488 DLGMYVGVKRSKYEEGKSWGVKYF-KNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527

BLAST of CmoCh18G005010 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.2e-145
Identity = 264/541 (48.80%), Postives = 355/541 (65.62%), Query Frame = 0

Query: 6   MNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSSY 65
           MN S      +  ++    S  +  + A P ENFL+C S +  +  +   ++YT  +  Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLY 60

Query: 66  SDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLS 125
             +LN T++NLRF +  TPKPL+I+TP + SHIQA +LCSK  GLQIRTRSGGHD EG+S
Sbjct: 61  MSILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMS 120

Query: 126 YVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTVG 185
           Y++ +PF++VDL N++SI IDV S+TAWV++GATLGE+YY I EK+ +L+FP G CPTVG
Sbjct: 121 YISQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVG 180

Query: 186 VGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFGI 245
           VGGH SGGGYG L+R YGLAADN+IDA LV+ +GK  DRK MGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 246 VVAWKVNLVSVPATVTLCVVNKTVE-DGATKLIHQWQYVAHKLENNLFLGILLEGGNFT- 305
           + AWK+ LV+VP+  T+  V K +E  G  KL ++WQ +A+K + +L L       N T 
Sbjct: 241 IAAWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITD 300

Query: 306 -RQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQ- 365
               +K      F S + G  D L+ ++N +FPEL + K DC E SW++T +     +  
Sbjct: 301 NHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNF 360

Query: 366 --PGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYG 425
                K E LL ++     +  +K DYVK+PIP  A+  I ++L   D+     +  PYG
Sbjct: 361 NTANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYG 420

Query: 426 GKMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPR 485
           G M EISE   PF HR    Y++ YT SW ++  +   KH+NW+R +Y++  P+VS++PR
Sbjct: 421 GIMEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPR 480

Query: 486 AAYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSI 541
            AYLNYRDLD+G  N     +Y QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSI
Sbjct: 481 LAYLNYRDLDLGKTNHASPNNYTQARIWGEKYFGK-NFNRLVKVKTKVDPNNFFRNEQSI 538

BLAST of CmoCh18G005010 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.6e-145
Identity = 256/519 (49.33%), Postives = 346/519 (66.67%), Query Frame = 0

Query: 28  VSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSSYSDVLNFTLRNLRFSTPHTPKPL 87
           +  + A P ENFL+C S Y  +  +    +YT  +  Y  VLN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLSYVASLPFIIVDLINLNSISIDV 147
           +I+TP +VSHIQA++LCSK  GLQIRTRSGGHD EGLSY++ +PF IVDL N++++ +D+
Sbjct: 83  VIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDI 142

Query: 148 ESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTVGVGGHLSGGGYGLLLRKYGLAAD 207
            S+TAWV++GATLGE+YY I E + + +FP G CPTVGVGGH SGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFGIVVAWKVNLVSVPATVTLCVVNK 267
           N+IDA LV+ +GK  DRK MGEDLFWAIRGGGG +FGI+ AWK+ LV VP+  T+  V K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKK 262

Query: 268 TVE-DGATKLIHQWQYVAHKLENNLFLGILLEGGNFT--RQGSKINPLALFFSFYLGKAD 327
            +E  G  KL ++WQ +A+K + +L L       N T     +K      F S +LG  D
Sbjct: 263 NMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVD 322

Query: 328 ELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQPGS---KLEDLLSKTPLMTVSTK 387
            L+ ++N +FPEL + K DC E SW++T +     +   +   K E LL ++     +  
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFS 382

Query: 388 VKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSEISELETPFSHRGEYSYK 447
           +K DYVK+ IP  A+  I ++L   ++     +  PYGG M EISE   PF HR    Y+
Sbjct: 383 IKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYE 442

Query: 448 IGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLNYRDLDIGSNNKYGRTSY 507
           + YT +W ++  +   KH+NW+R +Y++  P+VS++PR AYLNYRDLD+G  N     +Y
Sbjct: 443 LWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNY 502

Query: 508 EQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 541
            QA IWG KYFGK NF+RLV VKTK DP +FFR+EQSIP
Sbjct: 503 TQARIWGEKYFGK-NFNRLVKVKTKADPNNFFRNEQSIP 538

BLAST of CmoCh18G005010 vs. ExPASy TrEMBL
Match: A0A6J1GRE5 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456787 PE=3 SV=1)

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
           IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI
Sbjct: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG
Sbjct: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360
           NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL
Sbjct: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360

Query: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420
           QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG
Sbjct: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420

Query: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480
           KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA
Sbjct: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TFSSK 546
           TFSSK
Sbjct: 541 TFSSK 545

BLAST of CmoCh18G005010 vs. ExPASy TrEMBL
Match: A0A6J1GTA5 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456788 PE=3 SV=1)

HSP 1 Score: 1066.6 bits (2757), Expect = 3.4e-308
Identity = 527/545 (96.70%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSP LP SLT LV CSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP
Sbjct: 1   MSNSAMNSSPFLPFSLTFLVFCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
           IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI
Sbjct: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFL ILLEGG
Sbjct: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLSILLEGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360
           NFTRQGSKINPLALFFS YLGKADELLTILNTTFPEL LSK DC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGSKINPLALFFSLYLGKADELLTILNTTFPELGLSKTDCMETSWVETALIMGTGL 360

Query: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420
           QPGSKLEDLLSKTPLMTV TK+KSDYVKEPIPMAAIEGI QRLRSGDIETPQIIFIPYGG
Sbjct: 361 QPGSKLEDLLSKTPLMTVRTKMKSDYVKEPIPMAAIEGIWQRLRSGDIETPQIIFIPYGG 420

Query: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480
           KMSEISELETPFSHRGEYSYKIGYTT+W+E GIDAENKHLNWIRELYSYMAPFVSKSPRA
Sbjct: 421 KMSEISELETPFSHRGEYSYKIGYTTNWIEGGIDAENKHLNWIRELYSYMAPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRT+YEQA IWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTNYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TFSSK 546
           T SSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005010 vs. ExPASy TrEMBL
Match: A0A6J1K2F5 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490442 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 4.3e-287
Identity = 488/545 (89.54%), Postives = 509/545 (93.39%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNF KPH+NFLQCLSHYSQD SSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
            EGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIA+KSR+LAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGGYG+LLRKYGLAADNVIDAQLVDANGKF +RKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKV LV VPA+VTLC+ NKT EDGATKLIHQWQYVAHKLENN FLGILL GG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360
           NFTRQG KINPLALFFS YLGKADELLTILNTTFPEL LSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420
           Q GSKLEDLLSK PL+  STK+KSDY+KEPIPMAAIEGI +RLR  DIE PQ IF+PYGG
Sbjct: 361 QLGSKLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480
           KM +I E ETPFSHRGEY YKIGY  +W E+GI+AE +H++WIRELYSYM PFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQA IWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TFSSK 546
           T SSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005010 vs. ExPASy TrEMBL
Match: A0A6J1GRG1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456798 PE=3 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.1e-285
Identity = 486/549 (88.52%), Postives = 511/549 (93.08%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVS+NF KPHENFLQCLSHYSQD SSISNVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
            EGLSYVASLPFIIVDLINLNSISIDV+SKTAWVQSGATLGELYY IA+KS++L FPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVKSKTAWVQSGATLGELYYSIAKKSQTLTFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGGYG LLRKYGLAADNVIDAQLVDANGKF +RKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKV LVSVPA+VTLC+VNKT EDGATKLIHQWQYVAHKLEN++FLGILL GG
Sbjct: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGT-- 360
           NFTRQG KINP+ALFFS YLGKADEL+ ILNTTFPEL LSKKDC+ETSWVETA+IMGT  
Sbjct: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 --GLQPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFI 420
             GLQPGSKLEDLLSKT  +  STK+KSDYVKEPIPMAAIEGI +RLRS DIE PQ IF+
Sbjct: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420

Query: 421 PYGGKMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSK 480
           PYGG+M +ISE ETPFSHRGEY YKIGY   WVE+GIDAE +H++WIRELYSYM PFVSK
Sbjct: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQA IWGYKYFG ENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGNENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTFSSK 546
           QSIPT SSK
Sbjct: 541 QSIPTLSSK 549

BLAST of CmoCh18G005010 vs. ExPASy TrEMBL
Match: A0A6J1GRE1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456783 PE=3 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.4e-282
Identity = 481/545 (88.26%), Postives = 506/545 (92.84%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNF KPH+NFLQCLSHYSQD SSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
            EGLSYVASLPFIIVDLINLNSISIDVE+KTAWVQSGATLGELYY IA+KS++LAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGGYG+LLRKYGLAADNVIDAQLVDANGKF +R+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKV LV VPA+VTLC+ NKT EDGATKLIHQWQYVAHKLENN FLGILL GG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360
           NFTRQG KINPLALFFS YLGKA+ELLTILNTTFPEL LSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420
           Q GSKLEDLLSK  L+  STK+KSDY+KEPIPMAAIEGI +RLR  DIE PQ IF+PYGG
Sbjct: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480
           KM +I E ETPFSHRGE  YKIGY  +W E+GI+AE +H++WIRELYSYM PFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQA IWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TFSSK 546
           T SSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005010 vs. NCBI nr
Match: XP_022954548.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
           IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI
Sbjct: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG
Sbjct: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360
           NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL
Sbjct: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360

Query: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420
           QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG
Sbjct: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420

Query: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480
           KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA
Sbjct: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TFSSK 546
           TFSSK
Sbjct: 541 TFSSK 545

BLAST of CmoCh18G005010 vs. NCBI nr
Match: XP_022954549.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 1066.6 bits (2757), Expect = 7.0e-308
Identity = 527/545 (96.70%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSP LP SLT LV CSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP
Sbjct: 1   MSNSAMNSSPFLPFSLTFLVFCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
           IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI
Sbjct: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFL ILLEGG
Sbjct: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLSILLEGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360
           NFTRQGSKINPLALFFS YLGKADELLTILNTTFPEL LSK DC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGSKINPLALFFSLYLGKADELLTILNTTFPELGLSKTDCMETSWVETALIMGTGL 360

Query: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420
           QPGSKLEDLLSKTPLMTV TK+KSDYVKEPIPMAAIEGI QRLRSGDIETPQIIFIPYGG
Sbjct: 361 QPGSKLEDLLSKTPLMTVRTKMKSDYVKEPIPMAAIEGIWQRLRSGDIETPQIIFIPYGG 420

Query: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480
           KMSEISELETPFSHRGEYSYKIGYTT+W+E GIDAENKHLNWIRELYSYMAPFVSKSPRA
Sbjct: 421 KMSEISELETPFSHRGEYSYKIGYTTNWIEGGIDAENKHLNWIRELYSYMAPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRT+YEQA IWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTNYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TFSSK 546
           T SSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005010 vs. NCBI nr
Match: KAG6573421.1 (Cannabidiolic acid synthase-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1054.3 bits (2725), Expect = 3.6e-304
Identity = 521/545 (95.60%), Postives = 531/545 (97.43%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSP L LSLT LVLCSCSSWVSSNFAKPHENFL+CLSHYSQDASSISNVLYTP
Sbjct: 1   MSNSAMNSSPFLHLSLTFLVLCSCSSWVSSNFAKPHENFLRCLSHYSQDASSISNVLYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLNFTLRNLRFST HTPKPLLIITPLHVSHIQAA+LCSKS+GLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTSHTPKPLLIITPLHVSHIQAAILCSKSYGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
           IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI
Sbjct: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKVNLVSVPATVTLCVVN+T+EDGATKLIHQWQYVAHKLENNLFL ILLEGG
Sbjct: 241 GSFGIVVAWKVNLVSVPATVTLCVVNRTLEDGATKLIHQWQYVAHKLENNLFLSILLEGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360
           NFTRQGSKINPLALFFS YLGKADELLTILNTTFPEL LSK DC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGSKINPLALFFSLYLGKADELLTILNTTFPELGLSKTDCMETSWVETALIMGTGL 360

Query: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420
           QPGSKLEDLLSKTPLMTV TK+KSDYVKEPIPMAAIEGI QRLRSGDIETPQIIFIPYGG
Sbjct: 361 QPGSKLEDLLSKTPLMTVRTKMKSDYVKEPIPMAAIEGIWQRLRSGDIETPQIIFIPYGG 420

Query: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480
           KMSEISELETPFSHRGEYSYKIGYTT+W+E GIDAENKHLNWIRELYSYMAPFVSKSPRA
Sbjct: 421 KMSEISELETPFSHRGEYSYKIGYTTNWIEGGIDAENKHLNWIRELYSYMAPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQA IWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TFSSK 546
           T SSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005010 vs. NCBI nr
Match: XP_023542868.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1053.5 bits (2723), Expect = 6.2e-304
Identity = 518/545 (95.05%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQDASSIS +LYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSANFGKPHQNFLQCLSHYSQDASSISKILYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAA+LCSKS+GLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAILCSKSYGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
           IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIA+KSR+LAFPAGI
Sbjct: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIADKSRTLAFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKV LVSVPA VTLCVVN+T+EDGATKLIHQWQYVAHKLENNLFL ILLEGG
Sbjct: 241 GSFGIVVAWKVKLVSVPAKVTLCVVNRTLEDGATKLIHQWQYVAHKLENNLFLSILLEGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360
           NFTRQGSKINPLALFFS YLGKADELLTILNTTFPEL LSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGSKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420
           QPGSKLEDLLSKTPLMTV TK+KSDYVKEPIPMAAIEGI QRLRSGDIETPQIIFIPYGG
Sbjct: 361 QPGSKLEDLLSKTPLMTVRTKMKSDYVKEPIPMAAIEGIWQRLRSGDIETPQIIFIPYGG 420

Query: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480
           KMS+ISELETPFSHRGEYSYKIGYTT+WVE GIDAENKHLNWIRELYSYMAPFVSKSPRA
Sbjct: 421 KMSQISELETPFSHRGEYSYKIGYTTNWVEGGIDAENKHLNWIRELYSYMAPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQA IWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TFSSK 546
           T SSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005010 vs. NCBI nr
Match: XP_022994835.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 996.5 bits (2575), Expect = 9.0e-287
Identity = 488/545 (89.54%), Postives = 509/545 (93.39%), Query Frame = 0

Query: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60
           MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNF KPH+NFLQCLSHYSQD SSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180
            EGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIA+KSR+LAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGI 180

Query: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGGYG+LLRKYGLAADNVIDAQLVDANGKF +RKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300
           GSFGIVVAWKV LV VPA+VTLC+ NKT EDGATKLIHQWQYVAHKLENN FLGILL GG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360
           NFTRQG KINPLALFFS YLGKADELLTILNTTFPEL LSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420
           Q GSKLEDLLSK PL+  STK+KSDY+KEPIPMAAIEGI +RLR  DIE PQ IF+PYGG
Sbjct: 361 QLGSKLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480
           KM +I E ETPFSHRGEY YKIGY  +W E+GI+AE +H++WIRELYSYM PFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQA IWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TFSSK 546
           T SSK
Sbjct: 541 TLSSK 545

BLAST of CmoCh18G005010 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.8e-147
Identity = 267/535 (49.91%), Postives = 368/535 (68.79%), Query Frame = 0

Query: 9   SPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSSYSDV 68
           S +L    T  +L S    VS+N +    +FLQCLS    D++ +S V++TP ++S+S V
Sbjct: 8   SSVLIFFTTSTLLLSIPHPVSANRSN-QSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 67

Query: 69  LNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLSYVA 128
           L  +++N RFS P  PKP+LI+TP+  S +Q+AV C++  G+ IRTRSGGHD EGLSYV 
Sbjct: 68  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 127

Query: 129 SLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTVGVGG 188
             PF+I+DL NL SI++DV++++ WVQ+GAT+GELYY I +K+R+LAFPAG+CPTVGVGG
Sbjct: 128 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 187

Query: 189 HLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFGIVVA 248
           H SGGGYG LLRK+GLAAD+VIDA++VDA G+  +R+ MGED FWAIRGGGG SF +V++
Sbjct: 188 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 247

Query: 249 WKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGGNFTRQGSK 308
           WK+ L++VP+TVT+  V K  E  A K+IH+WQ+VA K+ ++LF+ ++L       Q  K
Sbjct: 248 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYK 307

Query: 309 INPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQPGSKLED 368
               A F   YLG    LL ++N  FPEL L + DC E SW+E+ +      + G +  +
Sbjct: 308 NMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPIN 367

Query: 369 LLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSEISEL 428
           +L+K    +++ K KSD+V+EP+P  AI  + +RL+  + E  Q+IF P+GGKMSEI++ 
Sbjct: 368 VLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADY 427

Query: 429 ETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLNYRDL 488
           ETPF HR    Y+I Y   W  RG D + K++ W+  +Y  M+ FV+KSPR AY+N RDL
Sbjct: 428 ETPFPHRKGNIYEIQYLNYW--RG-DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDL 487

Query: 489 DIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTFS 544
           D+G      R+ YE+   WG KYF K NF+RLV VKT VDP DFF  EQSIP F+
Sbjct: 488 DLGMYVGVKRSKYEEGKSWGVKYF-KNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527

BLAST of CmoCh18G005010 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 500.0 bits (1286), Expect = 2.5e-141
Identity = 261/529 (49.34%), Postives = 352/529 (66.54%), Query Frame = 0

Query: 15  SLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSSYSDVLNFTLR 74
           +L L++      W SS+ +   E F QCL+  S     IS  ++   N SYS VL   +R
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 75  NLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLSYV--ASLPF 134
           NLRF+T  TPKP LII   H SH+QAA+ C K H LQ++ RSGGHD +GLSYV  +  PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 135 IIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTVGVGGHLSG 194
            ++D+ NL S+ +DV SKTAWVQ+GA LGE+YY I EKS++LA+PAGICPTVGVGGH+SG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 195 GGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFGIVVAWKVN 254
           GGYG ++RKYGL  DN IDA++VD NGK  DRKLMGEDL+WAI GGGGGS+G+V+A+K+N
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 255 LVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGGNFTRQGSKINPL 314
           LV VP  VT+  +++T+E  AT +IH+WQ VA KL + LF+  +++  N T    K    
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQK-TVR 303

Query: 315 ALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQPGSKLEDLLSK 374
             F + +LG    LL+ILN  FPEL L + DC ETSW+++ L   T +Q GS  E LL +
Sbjct: 304 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFW-TNIQVGSS-ETLLLQ 363

Query: 375 TPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSEISELETPF 434
                   K KSDYV+EPI    +E I +++   ++E P + F PYGG+M  IS   TPF
Sbjct: 364 RNQPVNYLKRKSDYVREPISRTGLESIWKKMI--ELEIPTMAFNPYGGEMGRISSTVTPF 423

Query: 435 SHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLNYRDLDIGS 494
            +R    +KI Y  +W  R     ++++   R+LY +M PFVSK+PR ++ NYRD+D+G 
Sbjct: 424 PYRAGNLWKIQYGANW--RDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGI 483

Query: 495 NNKYGR-TSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 541
           N+  G+ +SY +   +G KYF   NF+RLV +KT+VD  +FFR+EQSIP
Sbjct: 484 NSHNGKISSYVEGKRYGKKYFA-GNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of CmoCh18G005010 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 498.0 bits (1281), Expect = 9.3e-141
Identity = 256/528 (48.48%), Postives = 351/528 (66.48%), Query Frame = 0

Query: 17  TLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSSYSDVLNFTLRNL 76
           T+L+     S + S     HE+FL+CLS+   D +    V++T  +SS+  +L+ +++N 
Sbjct: 9   TILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNP 68

Query: 77  RFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLSYVA-SLPFIIV 136
           RFS   TPKP+ IITP+  S +Q  + C++ HG+ +RTRS GH  EGLSY+A + PF ++
Sbjct: 69  RFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVI 128

Query: 137 DLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTVGVGGHLSGGGY 196
           DL NL SIS+DV+++T WVQ+GAT GELYY I + ++SLAFPAGI PTVGVGG  SGGGY
Sbjct: 129 DLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGY 188

Query: 197 GLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFGIVVAWKVNLVS 256
           G LLRKYGLAADN+IDA +VDA+G+  DR+ MGED FWAIRGGGG SFG++++WKV LV 
Sbjct: 189 GTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVD 248

Query: 257 VPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGGNFTRQGSKINPLALF 316
           VP+T+T+  V KT +  A ++I +WQY A K+ ++LF+   LE  N      K    ALF
Sbjct: 249 VPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN------KNAVHALF 308

Query: 317 FSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQPGSKLEDLLSKTPL 376
              Y+G  + LL ++   FPEL L K+ C E SW+E+ L       P  +   +L+    
Sbjct: 309 TGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD--FPKGESLGVLTNRER 368

Query: 377 MTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSEISELETPFSHR 436
            ++S K K D+V+EPIP AAI+ I +RL + +    +II  P+GGKMSE++E ETPF HR
Sbjct: 369 TSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHR 428

Query: 437 GEYSYKIGYTTSWVE---RGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLNYRDLDIGS 496
           G   Y+I Y   W E   +     +K+L W+  +Y +M P+VSKSPR AY+N++D+D+G 
Sbjct: 429 GGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGM 488

Query: 497 NNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 541
                +T YE+   WG KYF K NF+RLV VKT+VDP DFF  EQSIP
Sbjct: 489 YLGKKKTKYEEGKSWGVKYF-KNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of CmoCh18G005010 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 496.9 bits (1278), Expect = 2.1e-140
Identity = 262/543 (48.25%), Postives = 355/543 (65.38%), Query Frame = 0

Query: 7   NSSPLLPLSLTLLVLCSCSSWV---SSNFAKPHENFLQCLSHYSQDASSISNVLYTPFN- 66
           NS PL  +S  ++ +   S +    +S+ A   + F+ C+   +  +  +   L+TP   
Sbjct: 3   NSKPLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKN 62

Query: 67  -SSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDI 126
            S ++ VL  T +NL+F     PKP  I  P+H S +QA+++CSK  G+  R RSGGHD 
Sbjct: 63  VSLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDF 122

Query: 127 EGLSYVASL--PFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAG 186
           E LSYV+ +  PFI++DL  L  I++D+ES +AWVQ GATLGELYYRIAEKS+   FPAG
Sbjct: 123 EALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAG 182

Query: 187 ICPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGG 246
           +C +VG+GG+++GGGYG L+RKYGLA DNV+D ++VDANGK  DR  MGEDLFWAIRGGG
Sbjct: 183 LCTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGG 242

Query: 247 GGSFGIVVAWKVNLVSVPATVTLCVVNKTVE-DGATKLIHQWQYVAHKLENNLFLGILLE 306
           G SFGIV+AWK+ LV VP TVT+  V KT+E D   K I +WQ ++ K+   + + ++L 
Sbjct: 243 GASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLR 302

Query: 307 GGNFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGT 366
                  G+K   +  +   +LG+   LL ++   FPEL L++KDC E SW+E AL  G 
Sbjct: 303 AAG--NDGNKTVTMT-YLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG- 362

Query: 367 GLQPGSKLEDLLS-KTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIP 426
           G   GS +E LL  K+PL     K  SD+VKEPIP+  ++GI +RL  G+  T  + + P
Sbjct: 363 GFPTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGN--TTFLNWTP 422

Query: 427 YGGKMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKS 486
           YGG MS+I E   PF HR    +KI Y  +W+E    +  K +NWI+E+Y+YMAP+VS +
Sbjct: 423 YGGMMSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRK-INWIKEIYNYMAPYVSSN 482

Query: 487 PRAAYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQ 541
           PR AY+NYRDLD G N    + ++ +A IWG KYF K NFDRLV +KTKVDP +FFRHEQ
Sbjct: 483 PRQAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYF-KGNFDRLVKIKTKVDPENFFRHEQ 537

BLAST of CmoCh18G005010 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 491.9 bits (1265), Expect = 6.7e-139
Identity = 254/539 (47.12%), Postives = 352/539 (65.31%), Query Frame = 0

Query: 8   SSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFN--SSY 67
           S PL   S+ +L     +   +S+ A   + F+ C+   +     +    + P    S +
Sbjct: 4   SKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMF 63

Query: 68  SDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLS 127
           S VL  T +NLRF     PKP  I +P+H SH+QA+++CSK   + +R RSGGHD EGLS
Sbjct: 64  SQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLS 123

Query: 128 YVASL--PFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPT 187
           YV+ +  PFI++DL  +  ++I+++  +AWVQSGAT+GELYYRIAEKS+   FPAG+C +
Sbjct: 124 YVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSS 183

Query: 188 VGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSF 247
           +G+GGH++GG YG ++RKYGL ADNV+DA++VDANGK  DR  MGED FWAIRGG GGSF
Sbjct: 184 LGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSF 243

Query: 248 GIVVAWKVNLVSVPATVTLCVVNKTV-EDGATKLIHQWQYVAHKLENNLFLGILLEGGNF 307
           GI++AWK+ LV VP TVT+  V KT+ +D   K+I +WQ VA KL   LF+ +L    N 
Sbjct: 244 GIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NV 303

Query: 308 TRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVET-ALIMGTGLQ 367
              G        + + +LG    L+ ++  +FPEL L+ KDCIE SW+E+ A I G    
Sbjct: 304 AGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTH 363

Query: 368 PGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGK 427
             + +  L  K+P   VS K KSD+VK PIP + ++GI ++L   DI  P +I+ PYGG 
Sbjct: 364 TPTNVL-LQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGM 423

Query: 428 MSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAA 487
           M++I E + PF HR    +K+ Y TSW++      ++H+NWIR+LYSYM P+VS +PR A
Sbjct: 424 MAKIPESQIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSNPREA 483

Query: 488 YLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 541
           Y+NYRDLD+G N K  +T  +QA +WG  YF K NF+RL+ +K KVDP +FFRHEQSIP
Sbjct: 484 YVNYRDLDLGRNTKDVKTCIKQAQVWGANYF-KNNFNRLMMIKAKVDPENFFRHEQSIP 534