CmoCh18G003710 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh18G003710
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pentatricopeptide repeat-containing protein
Location	Cmo_Chr18: 2414441 .. 2414659 (+)
RNA-Seq Expression	CmoCh18G003710
Synteny	CmoCh18G003710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACACTAAAGGTTGCTCACCTTCTGTTGTTACCTATACATCAATGATACATGGTTTGTGTGCAATTCAAGTACTTAAAGATATGATGGGCAAGGGTATTGCACCTAATGTATTTACTTACAGTACCCTAATGGATGGATTTTGCAAGGCTGGCCATTCTTTGCAAGCTAGTAGATTTCAAGAAGCTGCAAACTTCTTGGATGAGATGGTCATTTGA

mRNA sequence

ATGGACACTAAAGGTTGCTCACCTTCTGTTGTTACCTATACATCAATGATACATGGTTTGTGTGCAATTCAAGTACTTAAAGATATGATGGGCAAGGGTATTGCACCTAATGTATTTACTTACAGTACCCTAATGGATGGATTTTGCAAGGCTGGCCATTCTTTGCAAGCTAGTAGATTTCAAGAAGCTGCAAACTTCTTGGATGAGATGGTCATTTGA

Coding sequence (CDS)

ATGGACACTAAAGGTTGCTCACCTTCTGTTGTTACCTATACATCAATGATACATGGTTTGTGTGCAATTCAAGTACTTAAAGATATGATGGGCAAGGGTATTGCACCTAATGTATTTACTTACAGTACCCTAATGGATGGATTTTGCAAGGCTGGCCATTCTTTGCAAGCTAGTAGATTTCAAGAAGCTGCAAACTTCTTGGATGAGATGGTCATTTGA

Protein sequence

MDTKGCSPSVVTYTSMIHGLCAIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGHSLQASRFQEAANFLDEMVI

Homology

[Close]

BLAST of CmoCh18G003710 vs. ExPASy Swiss-Prot
Match: Q9FNL2 (Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana OX=3702 GN=At5g46100 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.3e-14
Identity = 39/69 (56.52%), Postives = 48/69 (69.57%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 60
           M  K C+P+VVTYTS+I+GLC       A++ L++M  KGI PNVFTYS+LMDG CK G 
Sbjct: 218 MVEKDCAPTVVTYTSLINGLCGSKNVDEAMRYLEEMKSKGIEPNVFTYSSLMDGLCKDGR 277

Query: 61  SLQASRFQE 63
           SLQA    E
Sbjct: 278 SLQAMELFE 286

[Close]

BLAST of CmoCh18G003710 vs. ExPASy Swiss-Prot
Match: Q3ECK2 (Pentatricopeptide repeat-containing protein At1g62680, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g62680 PE=2 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 2.4e-09
Identity = 31/69 (44.93%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 60
           ++ KG  P+VVTYT++++GLC       A ++L DM+ K I PNV TYS L+D F K G 
Sbjct: 216 IERKGIRPNVVTYTALVNGLCNSSRWSDAARLLSDMIKKKITPNVITYSALLDAFVKNGK 275

Query: 61  SLQASRFQE 63
            L+A    E
Sbjct: 276 VLEAKELFE 284

[Close]

BLAST of CmoCh18G003710 vs. ExPASy Swiss-Prot
Match: Q0WVK7 (Pentatricopeptide repeat-containing protein At1g05670, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g05670 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 9.2e-09
Identity = 27/60 (45.00%), Postives = 43/60 (71.67%), Query Frame = 0


Query: 5   GCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGHSLQA 58
           GCSP+VVTYT++I GLC       A ++L +M   G+ PN+FTY+++++G CK+G+  +A
Sbjct: 451 GCSPNVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPNIFTYNSIVNGLCKSGNIEEA 510

[Close]

BLAST of CmoCh18G003710 vs. ExPASy Swiss-Prot
Match: Q9FIX3 (Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana OX=3702 GN=EMB2745 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.1e-08
Identity = 28/58 (48.28%), Postives = 41/58 (70.69%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKA 52
           M+  G SPSVVTY ++I+G C       AI VL+DM  KG++P+V +YST++ GFC++
Sbjct: 406 MNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRS 463

[Close]

BLAST of CmoCh18G003710 vs. ExPASy Swiss-Prot
Match: Q9FMF6 (Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g64320 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.1e-08
Identity = 32/73 (43.84%), Postives = 42/73 (57.53%), Query Frame = 0


Query: 5   GCSPSVVTYTSMIHGL-------CAIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGHSLQA 64
           G  P V TY S+I+G         A++VL DM  KG  PNV++Y+ L+DGFCK G     
Sbjct: 384 GIVPDVCTYNSLIYGYWKEGLVGLALEVLHDMRNKGCKPNVYSYTILVDGFCKLG----- 443

Query: 65  SRFQEAANFLDEM 71
            +  EA N L+EM
Sbjct: 444 -KIDEAYNVLNEM 450

[Close]

BLAST of CmoCh18G003710 vs. ExPASy TrEMBL
Match: A0A6J1CDW1 (pentatricopeptide repeat-containing protein At5g46100 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010648 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.9e-18
Identity = 47/64 (73.44%), Postives = 54/64 (84.38%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 58
           M+TKGCSPSVVTYTS+IHGLC       A+ +L+DMMGKGI PNVFTYS+LMDGFCKAGH
Sbjct: 236 METKGCSPSVVTYTSLIHGLCQLNNVDEAVGLLEDMMGKGIEPNVFTYSSLMDGFCKAGH 295

[Close]

BLAST of CmoCh18G003710 vs. ExPASy TrEMBL
Match: A0A6J1CF14 (pentatricopeptide repeat-containing protein At5g46100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010648 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.9e-18
Identity = 47/64 (73.44%), Postives = 54/64 (84.38%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 58
           M+TKGCSPSVVTYTS+IHGLC       A+ +L+DMMGKGI PNVFTYS+LMDGFCKAGH
Sbjct: 217 METKGCSPSVVTYTSLIHGLCQLNNVDEAVGLLEDMMGKGIEPNVFTYSSLMDGFCKAGH 276

[Close]

BLAST of CmoCh18G003710 vs. ExPASy TrEMBL
Match: A0A6J1KLZ3 (pentatricopeptide repeat-containing protein At5g46100 OS=Cucurbita maxima OX=3661 GN=LOC111495767 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.7e-18
Identity = 47/64 (73.44%), Postives = 53/64 (82.81%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 58
           M+ KGCSPSVVTYTSMIHGLC       A+ +L+DMM KGI PNVFTYS+LMDGFCKAGH
Sbjct: 217 MEKKGCSPSVVTYTSMIHGLCQLNNVDEAMDLLEDMMSKGIEPNVFTYSSLMDGFCKAGH 276

[Close]

BLAST of CmoCh18G003710 vs. ExPASy TrEMBL
Match: A0A6J1EKD3 (pentatricopeptide repeat-containing protein At5g46100 OS=Cucurbita moschata OX=3662 GN=LOC111434127 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-17
Identity = 46/64 (71.88%), Postives = 53/64 (82.81%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 58
           M+ KGCSPSV+TYTSMIHGLC       A+ +L+DMM KGI PNVFTYS+LMDGFCKAGH
Sbjct: 217 MEKKGCSPSVITYTSMIHGLCQLNNVDEAMDLLEDMMSKGIEPNVFTYSSLMDGFCKAGH 276

[Close]

BLAST of CmoCh18G003710 vs. ExPASy TrEMBL
Match: A0A2N9FIY0 (Senescence domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS14937 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.1e-16
Identity = 49/84 (58.33%), Postives = 58/84 (69.05%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 60
           M+TKGC PSVVTY+S+IHGLC       A+ +L++M+ K I PNVFTYS LMDGFCK G 
Sbjct: 605 METKGCLPSVVTYSSLIHGLCQSNDLDEALGLLEEMISKCIEPNVFTYSALMDGFCKGGR 664

Query: 61  SLQASRFQE-----AANFLDEMVI 73
           S QA    E      ANFLDEMV+
Sbjct: 665 SSQAMELLEMMISKPANFLDEMVL 688

[Close]

BLAST of CmoCh18G003710 vs. NCBI nr
Match: KAG7012455.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 118.6 bits (296), Expect = 2.2e-23
Identity = 55/64 (85.94%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1  MDTKGCSPSVVTYTSMIHGLCAIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGHSLQASRF 60
          M+TKGCSPSVVTYTSMIHGLCA+QVL+DMMGKGIAPNVFTYSTL+DGFCKAGHSLQA   
Sbjct: 1  METKGCSPSVVTYTSMIHGLCAMQVLEDMMGKGIAPNVFTYSTLVDGFCKAGHSLQARDL 60

Query: 61 QEAA 65
           E A
Sbjct: 61 LEVA 64

[Close]

BLAST of CmoCh18G003710 vs. NCBI nr
Match: XP_022139832.1 (pentatricopeptide repeat-containing protein At5g46100 isoform X2 [Momordica charantia] >XP_022139834.1 pentatricopeptide repeat-containing protein At5g46100 isoform X2 [Momordica charantia])

HSP 1 Score: 100.1 bits (248), Expect = 8.0e-18
Identity = 47/64 (73.44%), Postives = 54/64 (84.38%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 58
           M+TKGCSPSVVTYTS+IHGLC       A+ +L+DMMGKGI PNVFTYS+LMDGFCKAGH
Sbjct: 217 METKGCSPSVVTYTSLIHGLCQLNNVDEAVGLLEDMMGKGIEPNVFTYSSLMDGFCKAGH 276

[Close]

BLAST of CmoCh18G003710 vs. NCBI nr
Match: XP_022139830.1 (pentatricopeptide repeat-containing protein At5g46100 isoform X1 [Momordica charantia] >XP_022139831.1 pentatricopeptide repeat-containing protein At5g46100 isoform X1 [Momordica charantia])

HSP 1 Score: 100.1 bits (248), Expect = 8.0e-18
Identity = 47/64 (73.44%), Postives = 54/64 (84.38%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 58
           M+TKGCSPSVVTYTS+IHGLC       A+ +L+DMMGKGI PNVFTYS+LMDGFCKAGH
Sbjct: 236 METKGCSPSVVTYTSLIHGLCQLNNVDEAVGLLEDMMGKGIEPNVFTYSSLMDGFCKAGH 295

[Close]

BLAST of CmoCh18G003710 vs. NCBI nr
Match: XP_023001715.1 (pentatricopeptide repeat-containing protein At5g46100 [Cucurbita maxima])

HSP 1 Score: 99.0 bits (245), Expect = 1.8e-17
Identity = 47/64 (73.44%), Postives = 53/64 (82.81%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 58
           M+ KGCSPSVVTYTSMIHGLC       A+ +L+DMM KGI PNVFTYS+LMDGFCKAGH
Sbjct: 217 MEKKGCSPSVVTYTSMIHGLCQLNNVDEAMDLLEDMMSKGIEPNVFTYSSLMDGFCKAGH 276

[Close]

BLAST of CmoCh18G003710 vs. NCBI nr
Match: XP_022927208.1 (pentatricopeptide repeat-containing protein At5g46100 [Cucurbita moschata])

HSP 1 Score: 98.6 bits (244), Expect = 2.3e-17
Identity = 46/64 (71.88%), Postives = 53/64 (82.81%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 58
           M+ KGCSPSV+TYTSMIHGLC       A+ +L+DMM KGI PNVFTYS+LMDGFCKAGH
Sbjct: 217 MEKKGCSPSVITYTSMIHGLCQLNNVDEAMDLLEDMMSKGIEPNVFTYSSLMDGFCKAGH 276

[Close]

BLAST of CmoCh18G003710 vs. TAIR 10
Match: AT5G46100.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 3.0e-15
Identity = 39/69 (56.52%), Postives = 48/69 (69.57%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 60
           M  K C+P+VVTYTS+I+GLC       A++ L++M  KGI PNVFTYS+LMDG CK G 
Sbjct: 218 MVEKDCAPTVVTYTSLINGLCGSKNVDEAMRYLEEMKSKGIEPNVFTYSSLMDGLCKDGR 277

Query: 61  SLQASRFQE 63
           SLQA    E
Sbjct: 278 SLQAMELFE 286

[Close]

BLAST of CmoCh18G003710 vs. TAIR 10
Match: AT1G62680.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.7e-10
Identity = 31/69 (44.93%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGH 60
           ++ KG  P+VVTYT++++GLC       A ++L DM+ K I PNV TYS L+D F K G 
Sbjct: 216 IERKGIRPNVVTYTALVNGLCNSSRWSDAARLLSDMIKKKITPNVITYSALLDAFVKNGK 275

Query: 61  SLQASRFQE 63
            L+A    E
Sbjct: 276 VLEAKELFE 284

[Close]

BLAST of CmoCh18G003710 vs. TAIR 10
Match: AT1G05670.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 6.6e-10
Identity = 27/60 (45.00%), Postives = 43/60 (71.67%), Query Frame = 0


Query: 5   GCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGHSLQA 58
           GCSP+VVTYT++I GLC       A ++L +M   G+ PN+FTY+++++G CK+G+  +A
Sbjct: 451 GCSPNVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPNIFTYNSIVNGLCKSGNIEEA 510

[Close]

BLAST of CmoCh18G003710 vs. TAIR 10
Match: AT1G05670.2 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 6.6e-10
Identity = 27/60 (45.00%), Postives = 43/60 (71.67%), Query Frame = 0


Query: 5   GCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKAGHSLQA 58
           GCSP+VVTYT++I GLC       A ++L +M   G+ PN+FTY+++++G CK+G+  +A
Sbjct: 451 GCSPNVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPNIFTYNSIVNGLCKSGNIEEA 510

[Close]

BLAST of CmoCh18G003710 vs. TAIR 10
Match: AT5G39710.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 1.5e-09
Identity = 28/58 (48.28%), Postives = 41/58 (70.69%), Query Frame = 0


Query: 1   MDTKGCSPSVVTYTSMIHGLC-------AIQVLKDMMGKGIAPNVFTYSTLMDGFCKA 52
           M+  G SPSVVTY ++I+G C       AI VL+DM  KG++P+V +YST++ GFC++
Sbjct: 406 MNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRS 463

The following BLAST results are available for this feature:

BLAST of CmoCh18G003710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FNL2	4.3e-14	56.52	Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana OX...	[more]
Q3ECK2	2.4e-09	44.93	Pentatricopeptide repeat-containing protein At1g62680, mitochondrial OS=Arabidop...	[more]
Q0WVK7	9.2e-09	45.00	Pentatricopeptide repeat-containing protein At1g05670, mitochondrial OS=Arabidop...	[more]
Q9FIX3	2.1e-08	48.28	Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana OX...	[more]
Q9FMF6	2.1e-08	43.84	Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidop...	[more]

BLAST of CmoCh18G003710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDW1	3.9e-18	73.44	pentatricopeptide repeat-containing protein At5g46100 isoform X1 OS=Momordica ch...	[more]
A0A6J1CF14	3.9e-18	73.44	pentatricopeptide repeat-containing protein At5g46100 isoform X2 OS=Momordica ch...	[more]
A0A6J1KLZ3	8.7e-18	73.44	pentatricopeptide repeat-containing protein At5g46100 OS=Cucurbita maxima OX=366...	[more]
A0A6J1EKD3	1.1e-17	71.88	pentatricopeptide repeat-containing protein At5g46100 OS=Cucurbita moschata OX=3...	[more]
A0A2N9FIY0	2.1e-16	58.33	Senescence domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS149...	[more]

BLAST of CmoCh18G003710 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012455.1	2.2e-23	85.94	Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub...	[more]
XP_022139832.1	8.0e-18	73.44	pentatricopeptide repeat-containing protein At5g46100 isoform X2 [Momordica char...	[more]
XP_022139830.1	8.0e-18	73.44	pentatricopeptide repeat-containing protein At5g46100 isoform X1 [Momordica char...	[more]
XP_023001715.1	1.8e-17	73.44	pentatricopeptide repeat-containing protein At5g46100 [Cucurbita maxima]	[more]
XP_022927208.1	2.3e-17	71.88	pentatricopeptide repeat-containing protein At5g46100 [Cucurbita moschata]	[more]

BLAST of CmoCh18G003710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46100.1	3.0e-15	56.52	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT1G62680.1	1.7e-10	44.93	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT1G05670.1	6.6e-10	45.00	Pentatricopeptide repeat (PPR-like) superfamily protein	[more]
AT1G05670.2	6.6e-10	45.00	Pentatricopeptide repeat (PPR-like) superfamily protein	[more]
AT5G39710.1	1.5e-09	48.28	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002885	Pentatricopeptide repeat	PFAM	PF12854	PPR_1	coord: 4..21 e-value: 5.0E-7 score: 29.3 coord: 32..70 e-value: 3.2E-10 score: 39.5
IPR002885	Pentatricopeptide repeat	TIGRFAM	TIGR00756	TIGR00756	coord: 39..53 e-value: 0.0011 score: 16.9
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 1..72 e-value: 2.7E-16 score: 61.9
None	No IPR available	PANTHER	PTHR47933:SF25	EMP16	coord: 1..57
None	No IPR available	PANTHER	PTHR47933	PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL	coord: 1..57

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh18G003710.1	CmoCh18G003710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding