CmoCh18G001770 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh18G001770
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionProtein kinase domain-containing protein
LocationCmo_Chr18: 1217572 .. 1221093 (-)
RNA-Seq ExpressionCmoCh18G001770
SyntenyCmoCh18G001770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAATATTTCTTCTCATCTCCTTCTTTCCGAACCCTCCAATTCTCTGGTTCTTTGATCATCTTTTTCAATTCGAGATTCTGCTTCTTACTTCGAGATCATGGAGATGGAGCGCATGATTGAGTTTCCTCACACGCATCTTGATCGGAGGCCCCGAAAGAGGGCTCGTTTGGGCTGGGACGTTGTCCCTGAGGCCCCTAAGGTATAATCTCCAATCCTCTCTTTCAGATCTGATTTTATTTTGGGTTGATTTTGGTTTTGTAGATCTAGATCTGCTGGATTTTTCTTTGTTTAGGTATGTATTAGTTTCACACGCCGAGGTATCATTTCTGATCAAAGTAGCTTAAGAAAGCCTTTCGTTCTTTGCTCTTTGTTATCTTAGATTTACTTTTTTTGATCGCTGATTCTCGTTTTTTACCATCGATATTCCTGATTATTCTAGGAGTATAGACTATTTTTCTTCAGCTTGATTTATCGAACTGTTGAGATAACGAGTTTCTATTTTATTTTTAAGCTCGAATGAATTATCTTGTCAGATCAGGTAAGTGTATTTTTATTTATTTATTTGGATGGATGCTTATATTTCTCTTTTGATGTATGATTTTTCCTTGTTATTTCTCTTCCTCTCCACGAATTGTTTAAATTCGGTGTTTCCCTTTCCTTGCTTATCTTCCGTGTTTATTTGCCATGTATCTGCCTTTTATTTCATCACTCTCTGTATTTCCTCTCATCTGTATTCATTCAACAAAATCTTGCATTTTATTATGTGTTGTGGAAAATGAAGGCTGTGGGTGAGGATATTAGGTCTAGGAAGGGCAAACTTTTATATGAATGGGTGACTGTGTGTAGGCCCAGGTAGGAATATGTTGTGGACAAGAGATTGGGAATATGCCAAGCTTTGCATCTTCAAAAGCACCATCAGATCATTCTTCTGATCCACTATTTGTTAAGGGAGTGGCTCGAAATGGTTCTCCCCCTTGGCGAGAAGATGACAAAGATGGGCATTACATGTTTGCGCTTGGAGATAATTTAACTTCTCGCTGTAAATACACTTGACTATGATCAAATGAATTTTTATGTTGTTTCAGAATTTATCATCGTAGCTTTCTTCTCTTGTGATGAATAAGTTCCTTTGTTTATTTAAATGAAGGCTGCAATTTCTACTTTCGCTTCCAACTGAAGACTGTATTTTAAACAATGTTCATCATTGTCGCTTACTTTTATTTTCATTTTTCAAAATTTGCCTCATCATCGTTACTTTGGAATTGCTTAGGATAGTTGGGTTTTTGGAGATTATCATGTCTTTTCCTATGGTTGCTACTTAATCTTAATCTGACAATTTTTCTTTTGTGCCAGATAAAATACATAGCAAAATGGGTGAAGGTACATTTGGACAGGTTTTAGAATGCTGGGACAATGAAAGGAAGGAAATGGTTGCTATTAAAATTGTGCGTGGAATTAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTACAGCAACTTCAAAAGCATGATAAAGGTGGCAACCGGTGAGTATCTTTTTTGTTCTAAGTCTTCCTTACTGCCATGATTTGCTCTGTAGCTCTTGTTTTCTTTGAGGCTATGGTTAAATGGCTTTATTCCTTGCAGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATCTGTATTGTAAGTATAAGGCTGTTTTATCATAGATTAATTTCCCCCGATTCACTAAATATGGGGAATTAAGATTTTTGGGTGAACTTGGATGGTTTTAACAGGTGTTTGAGAAGCTTGGGCCAAGTTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAGTGTGTAGCATGTGTGTAGTATTTTATTGAACCTAGTCCTTTCATTTTCCTTTTTTGTAGTGTCTTGTTTTCATTGCAAAACACTCCGCACATTTCTTCCAGTTGAATGGTTATTAGTATCTGTTGTACAACGTTTCCTGCAATTTTATTTAAAATGTATGTCTAGTCAGTTCTAATTTTTTGTTTTGTATCCTAATGATTTCTTTGATACTTGAAATCCAGTCATGCATGACCTACGAATGGTACATACTGACTTGAAGCCTGAGAACGTGCTGTTGGTCTCTGCAGATTATGTGAAAGTACACGACTATAAGGTAATTATACTTGTTTTATGATAGTTTCTTGGATTGTTCCTACTTTTAGTTCTCTGGGTAAGCTTTGCTAATTACTTTGACCATTCGCAGCAGAATTTGTCTCGGTCACCAAGGGATAGTTCTAACTTCAAAAGAGTGCCCAAGTCAAGTGCTATAAAGGTGATTGATTTTGGTAGCACCACTTATGATCGTCCAGATCAGAATTACATCGTTTCAACACGCCATTATCGAGCACCAGAGGTTATCCTTGGTAAGGAATTTTGATTTTGGTAGTGCTACTTCCGGTGTTTTTATTTATGTTGAACTTGACCATATTATTAACTGTGTAGGTCTTGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGTATGTGTCTTGTTTGTATGTAATACGACTTTAGCAGAGTAAGGTTCGGTCTTGTTGAGTTTCCAATTCAATATCTTTTTTAGGGTGAAGCACTCTTTCAAACTCACGAGAACTTGGAGCACTTGGCGATGATGGAGTGTGTGCTCGGTCCATTGCCACATGAGATGCTGAAGAGAGTAGAGTGAGTATTTAGCCCTTGGTGTGTGTGTGTTATGCAGTAAAGAAGAACCAAAAATGCTGAAATTTGTTCCTTTTCGTGTATCCATAGTCGGCATGCCGAAAAGTACGTTAGAAGAGGTAGATTAGATTGGCCAGAAGGAGCAACCTCGAGGGATAGTATGAAGGCTGTTCAAAAGCTGCCTCGTTTACCGGTTTGTATTTCAATGGTGTTTCGACATGGCTGGTGCCTTTCGTTTTTTCGACACTAATTATATTGTTGTTCAAACATATGCAGAACTTGATAATGCAGCATGTAGACCATTCGGCGGGCGAATTGATTCATCTTGTCCAAGGGTTGCTTCGATACGAACCTGCCGATAGACTAACGGCACATGAAGCCCTAAGGCACCCATTCTTTACAAGGGATCCCTTGAGGAGATTGTAGTTTTTATTAGTGGGGGACGTAATTAACTGACTAGCATAGCATTTGGGCTCTCTTCACACTCGACTTCATGCAAACCTGTGTATGGATGGATGGATGGACGGGCGGACGGACGGGCGGACGTATCTGCAAATTGTTTAACAAATACATGGATTTGGACGTGGTAGACAAAATTGTGAACAAAACACTTAATTTGTCTATATGAGTGCTTGTGTATATTAATTTTCATGTATATTTTTGTCTTCACTAACGTATTGGATTGGTAATCAGACAACACATTTGTTCATCAATATAATTACAACAATTATATTCTTCTATCTTTATCTCCG

mRNA sequence

AAGAATATTTCTTCTCATCTCCTTCTTTCCGAACCCTCCAATTCTCTGGTTCTTTGATCATCTTTTTCAATTCGAGATTCTGCTTCTTACTTCGAGATCATGGAGATGGAGCGCATGATTGAGTTTCCTCACACGCATCTTGATCGGAGGCCCCGAAAGAGGGCTCGTTTGGGCTGGGACGTTGTCCCTGAGGCCCCTAAGGCCCAGGTAGGAATATGTTGTGGACAAGAGATTGGGAATATGCCAAGCTTTGCATCTTCAAAAGCACCATCAGATCATTCTTCTGATCCACTATTTGTTAAGGGAGTGGCTCGAAATGGTTCTCCCCCTTGGCGAGAAGATGACAAAGATGGGCATTACATGTTTGCGCTTGGAGATAATTTAACTTCTCGCTATAAAATACATAGCAAAATGGGTGAAGGTACATTTGGACAGGTTTTAGAATGCTGGGACAATGAAAGGAAGGAAATGGTTGCTATTAAAATTGTGCGTGGAATTAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTACAGCAACTTCAAAAGCATGATAAAGGTGGCAACCGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATCTGTATTGTGTTTGAGAAGCTTGGGCCAAGTTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAGTGTGTAGCATTCATGCATGACCTACGAATGGTACATACTGACTTGAAGCCTGAGAACGTGCTGTTGGTCTCTGCAGATTATGTGAAAGTACACGACTATAAGAATTTGTCTCGGTCACCAAGGGATAGTTCTAACTTCAAAAGAGTGCCCAAGTCAAGTGCTATAAAGGTGATTGATTTTGGTAGCACCACTTATGATCGTCCAGATCAGAATTACATCGTTTCAACACGCCATTATCGAGCACCAGAGGTTATCCTTGGTCTTGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGGTGAAGCACTCTTTCAAACTCACGAGAACTTGGAGCACTTGGCGATGATGGAGTGTGTGCTCGGTCCATTGCCACATGAGATGCTGAAGAGAGTAGATCGGCATGCCGAAAAGTACGTTAGAAGAGGTAGATTAGATTGGCCAGAAGGAGCAACCTCGAGGGATAGTATGAAGGCTGTTCAAAAGCTGCCTCGTTTACCGAACTTGATAATGCAGCATGTAGACCATTCGGCGGGCGAATTGATTCATCTTGTCCAAGGGTTGCTTCGATACGAACCTGCCGATAGACTAACGGCACATGAAGCCCTAAGGCACCCATTCTTTACAAGGGATCCCTTGAGGAGATTGTAGTTTTTATTAGTGGGGGACGTAATTAACTGACTAGCATAGCATTTGGGCTCTCTTCACACTCGACTTCATGCAAACCTGTGTATGGATGGATGGATGGACGGGCGGACGGACGGGCGGACGTATCTGCAAATTGTTTAACAAATACATGGATTTGGACGTGGTAGACAAAATTGTGAACAAAACACTTAATTTGTCTATATGAGTGCTTGTGTATATTAATTTTCATGTATATTTTTGTCTTCACTAACGTATTGGATTGGTAATCAGACAACACATTTGTTCATCAATATAATTACAACAATTATATTCTTCTATCTTTATCTCCG

Coding sequence (CDS)

ATGGAGATGGAGCGCATGATTGAGTTTCCTCACACGCATCTTGATCGGAGGCCCCGAAAGAGGGCTCGTTTGGGCTGGGACGTTGTCCCTGAGGCCCCTAAGGCCCAGGTAGGAATATGTTGTGGACAAGAGATTGGGAATATGCCAAGCTTTGCATCTTCAAAAGCACCATCAGATCATTCTTCTGATCCACTATTTGTTAAGGGAGTGGCTCGAAATGGTTCTCCCCCTTGGCGAGAAGATGACAAAGATGGGCATTACATGTTTGCGCTTGGAGATAATTTAACTTCTCGCTATAAAATACATAGCAAAATGGGTGAAGGTACATTTGGACAGGTTTTAGAATGCTGGGACAATGAAAGGAAGGAAATGGTTGCTATTAAAATTGTGCGTGGAATTAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTACAGCAACTTCAAAAGCATGATAAAGGTGGCAACCGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATCTGTATTGTGTTTGAGAAGCTTGGGCCAAGTTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAGTGTGTAGCATTCATGCATGACCTACGAATGGTACATACTGACTTGAAGCCTGAGAACGTGCTGTTGGTCTCTGCAGATTATGTGAAAGTACACGACTATAAGAATTTGTCTCGGTCACCAAGGGATAGTTCTAACTTCAAAAGAGTGCCCAAGTCAAGTGCTATAAAGGTGATTGATTTTGGTAGCACCACTTATGATCGTCCAGATCAGAATTACATCGTTTCAACACGCCATTATCGAGCACCAGAGGTTATCCTTGGTCTTGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGGTGAAGCACTCTTTCAAACTCACGAGAACTTGGAGCACTTGGCGATGATGGAGTGTGTGCTCGGTCCATTGCCACATGAGATGCTGAAGAGAGTAGATCGGCATGCCGAAAAGTACGTTAGAAGAGGTAGATTAGATTGGCCAGAAGGAGCAACCTCGAGGGATAGTATGAAGGCTGTTCAAAAGCTGCCTCGTTTACCGAACTTGATAATGCAGCATGTAGACCATTCGGCGGGCGAATTGATTCATCTTGTCCAAGGGTTGCTTCGATACGAACCTGCCGATAGACTAACGGCACATGAAGCCCTAAGGCACCCATTCTTTACAAGGGATCCCTTGAGGAGATTGTAG

Protein sequence

MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDHSSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRRGRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEALRHPFFTRDPLRRL
Homology
BLAST of CmoCh18G001770 vs. ExPASy Swiss-Prot
Match: P51567 (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=1 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 1.8e-216
Identity = 358/428 (83.64%), Postives = 397/428 (92.76%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A KAQVG+ CGQEIGN+ SFASS APSD+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SS  L VKGVARNGSPPWREDDKDGHY+F LGD+LT RYKI+SKMGEGTFGQVLECWD E
Sbjct: 61  SSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVA+KIVRG++KYR+AAMIEIE+LQQL KHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           G SLYDFLRKNNYRSFPIDLVR+IG QLLECVAFMHDLRM+HTDLKPEN+LLVS+DYVK+
Sbjct: 181 GSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKI 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300
            +YK  SR  RD   +KRVPKSSAIKVIDFGSTTY+R DQ YIVSTRHYRAPEVILGLGW
Sbjct: 241 PEYKG-SRLQRDVC-YKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360
           S+PCD+WSVGCI+VELC+GEALFQTHENLEHLAMME VLGP P +MLK+VDRH+EKYVRR
Sbjct: 301 SYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420
           GRLDWP+GATSRDS+KAV KLPRL NLIMQHVDHSAGELI++VQGLLR++P++R+TA EA
Sbjct: 361 GRLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAREA 420

Query: 421 LRHPFFTR 429
           LRHPFF R
Sbjct: 421 LRHPFFAR 425

BLAST of CmoCh18G001770 vs. ExPASy Swiss-Prot
Match: P51566 (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=2 SV=2)

HSP 1 Score: 640.2 bits (1650), Expect = 1.7e-182
Identity = 304/430 (70.70%), Postives = 355/430 (82.56%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIGNMPSFASSKAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P +
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVP-N 76

Query: 61  HSSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDN 120
                +F  G+ R GSPPWR DDKDGHY+F +GD LT RY+I SKMGEGTFGQVLEC+DN
Sbjct: 77  FVYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR++HTDLKPEN+LLVS++Y+K
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++  D NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMME VLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

Query: 361 RG-RLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAH 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRY+P +R  A 
Sbjct: 377 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 436

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 437 EALNHPFFTR 445

BLAST of CmoCh18G001770 vs. ExPASy Swiss-Prot
Match: P51568 (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=1 SV=2)

HSP 1 Score: 510.8 bits (1314), Expect = 1.6e-143
Identity = 250/411 (60.83%), Postives = 311/411 (75.67%), Query Frame = 0

Query: 17  RPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDHSSDPLFVKGVARNGSP 76
           R RKR R+ WD  P  P+A+  +  G                 H SD        R  SP
Sbjct: 13  RVRKRPRMTWDEAPAEPEAKRAVIKG-----------------HGSD-------GRILSP 72

Query: 77  PWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNERKEMVAIKIVRGIRKY 136
           P R+DD+DGHY+F+L DNLT RYKI SKMGEGTFG+VLECWD + KE VAIKI+R I+KY
Sbjct: 73  PLRDDDRDGHYVFSLRDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKY 132

Query: 137 RDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSF 196
           RDAAMIEI+VLQ+L K DKG  RCVQ++NWFDYRNHICIVFEKLGPSL+DFL++N Y +F
Sbjct: 133 RDAAMIEIDVLQKLVKSDKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAF 192

Query: 197 PIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKVHDYKNLSRSPRDSSNF 256
           P+ LVRD G QLLE VA+MH+L++VHTDLKPEN+LLVS++ VK+ D K   RS  + ++F
Sbjct: 193 PLALVRDFGCQLLESVAYMHELQLVHTDLKPENILLVSSENVKLPDNK---RSAANETHF 252

Query: 257 KRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVEL 316
           + +PKSSAIK+IDFGST  D    + IV TRHYR+PEVILGLGWS+ CD+WS+GCIL EL
Sbjct: 253 RCLPKSSAIKLIDFGSTVCDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFEL 312

Query: 317 CSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRRG-RLDWPEGATSRDSM 376
           C+GEALFQTH+NLEHLAMME  LGPLP  M ++  R AEKY RRG RL+WPEGA SR+S+
Sbjct: 313 CTGEALFQTHDNLEHLAMMERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESI 372

Query: 377 KAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEALRHPFF 427
           +AV++L RL +++ +HVD++      L+ GLL Y+P++RLTA+EAL HPFF
Sbjct: 373 RAVKRLDRLKDMVSKHVDNTRSRFADLLYGLLAYDPSERLTANEALDHPFF 396

BLAST of CmoCh18G001770 vs. ExPASy Swiss-Prot
Match: P49760 (Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.8e-91
Identity = 171/355 (48.17%), Postives = 240/355 (67.61%), Query Frame = 0

Query: 80  EDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNER-KEMVAIKIVRGIRKYRD 139
           EDD +GH ++ +GD L  RY+I S +GEGTFG+V++C D+ R    VA+KI++ + KY++
Sbjct: 144 EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKE 203

Query: 140 AAMIEIEVLQQLQKHDKGG-NRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFP 199
           AA +EI VL+++ + D    N CVQ+ +WFDY  H+CI FE LG S +DFL+ NNY  +P
Sbjct: 204 AARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP 263

Query: 200 IDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKVHDYKNLSRSPRDSSNFK 259
           I  VR +  QL + V F+HD ++ HTDLKPEN+L V++DY   +   NL +  RD    +
Sbjct: 264 IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTY---NLEKK-RD----E 323

Query: 260 RVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELC 319
           R  KS+A++V+DFGS T+D    + IVSTRHYRAPEVIL LGWS PCD+WS+GCI+ E  
Sbjct: 324 RSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYY 383

Query: 320 SGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRRGRLDWPEGATS----RD 379
            G  LFQTH+N EHLAMME +LGP+P  M+++  +  +KY  RGRLDW E  ++    R+
Sbjct: 384 VGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRK--QKYFYRGRLDWDENTSAGRYVRE 443

Query: 380 SMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEALRHPFFTR 429
           + K +++      L  +  +H   +L  L++ +L YEPA RLT  EAL+HPFF R
Sbjct: 444 NCKPLRRY-----LTSEAEEHH--QLFDLIESMLEYEPAKRLTLGEALQHPFFAR 481

BLAST of CmoCh18G001770 vs. ExPASy Swiss-Prot
Match: O35491 (Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2)

HSP 1 Score: 337.4 bits (864), Expect = 2.4e-91
Identity = 171/353 (48.44%), Postives = 239/353 (67.71%), Query Frame = 0

Query: 80  EDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNER-KEMVAIKIVRGIRKYRD 139
           EDD +GH ++ +GD L  RY+I S +GEGTFG+V++C D+ R    VA+KI++ + KY++
Sbjct: 143 EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGTQVALKIIKNVEKYKE 202

Query: 140 AAMIEIEVLQQLQKHDKGG-NRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFP 199
           AA +EI VL+++ + D    N CVQ+ +WFDY  H+CI FE LG S +DFL+ NNY  +P
Sbjct: 203 AARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP 262

Query: 200 IDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKVHDYKNLSRSPRDSSNFK 259
           I  VR +  QL + V F+HD ++ HTDLKPEN+L V++DY   +   NL +  RD    +
Sbjct: 263 IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTY---NLEKK-RD----E 322

Query: 260 RVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELC 319
           R  KS+A++V+DFGS T+D    + IVSTRHYRAPEVIL LGWS PCD+WS+GCI+ E  
Sbjct: 323 RSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYY 382

Query: 320 SGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRRGRLDWPEGATS----RD 379
            G  LFQTH+N EHLAMME +LGP+P  M+++  +  +KY  RGRLDW E  ++    R+
Sbjct: 383 VGFTLFQTHDNREHLAMMERILGPVPSRMIRKTRK--QKYFYRGRLDWDENTSAGRYVRE 442

Query: 380 SMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEALRHPFF 427
           + K +++      L  +  DH   +L  L++ +L YEPA RLT  EAL+HPFF
Sbjct: 443 NCKPLRRY-----LTSEAEDHH--QLFDLIENMLEYEPAKRLTLGEALQHPFF 478

BLAST of CmoCh18G001770 vs. ExPASy TrEMBL
Match: A0A6J1GS08 (serine/threonine-protein kinase AFC2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456990 PE=4 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 4.3e-261
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE
Sbjct: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420
           GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA
Sbjct: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420

Query: 421 LRHPFFTRDPLRRL 435
           LRHPFFTRDPLRRL
Sbjct: 421 LRHPFFTRDPLRRL 434

BLAST of CmoCh18G001770 vs. ExPASy TrEMBL
Match: A0A6J1GS36 (serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456990 PE=4 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.0e-259
Identity = 434/435 (99.77%), Postives = 434/435 (99.77%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE
Sbjct: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240

Query: 241 HDYK-NLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300
           HDYK NLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG
Sbjct: 241 HDYKQNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360
           WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR
Sbjct: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360

Query: 361 RGRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE 420
           RGRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE
Sbjct: 361 RGRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE 420

Query: 421 ALRHPFFTRDPLRRL 435
           ALRHPFFTRDPLRRL
Sbjct: 421 ALRHPFFTRDPLRRL 435

BLAST of CmoCh18G001770 vs. ExPASy TrEMBL
Match: A0A6J1K5G9 (serine/threonine-protein kinase AFC2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490237 PE=4 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.0e-258
Identity = 431/434 (99.31%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE
Sbjct: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDL MVHTDLKPENVLLVSADYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLLMVHTDLKPENVLLVSADYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMME VLGPLPHEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPHEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420
           GRLDWPEGATSRDS+KAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420

Query: 421 LRHPFFTRDPLRRL 435
           LRHPFFTRDPLRRL
Sbjct: 421 LRHPFFTRDPLRRL 434

BLAST of CmoCh18G001770 vs. ExPASy TrEMBL
Match: A0A6J1JW59 (serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490237 PE=4 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 4.9e-257
Identity = 431/435 (99.08%), Postives = 432/435 (99.31%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE
Sbjct: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDL MVHTDLKPENVLLVSADYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLLMVHTDLKPENVLLVSADYVKV 240

Query: 241 HDYK-NLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300
           HDYK NLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG
Sbjct: 241 HDYKQNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360
           WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMME VLGPLPHEMLKRVDRHAEKYVR
Sbjct: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPHEMLKRVDRHAEKYVR 360

Query: 361 RGRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE 420
           RGRLDWPEGATSRDS+KAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE
Sbjct: 361 RGRLDWPEGATSRDSIKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE 420

Query: 421 ALRHPFFTRDPLRRL 435
           ALRHPFFTRDPLRRL
Sbjct: 421 ALRHPFFTRDPLRRL 435

BLAST of CmoCh18G001770 vs. ExPASy TrEMBL
Match: A0A6J1C5J1 (serine/threonine-protein kinase AFC2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008590 PE=4 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 5.4e-248
Identity = 411/433 (94.92%), Postives = 422/433 (97.46%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEI NMPSFASS+APSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SS+PLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRY+IHSKMGEGTFGQVLECWD E
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYRIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQL KHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRM+HTDLKPEN+LLVS DYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSVDYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDS+NFKRVPKSSAIKVIDFGSTTYDR DQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSTNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMME VLGPLP EMLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKKVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420
           GRLDWPEGATSRDS+KAVQKLPRLPNLIMQHVDHSAGELI LVQGLLRY P DRLTA +A
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLPRLPNLIMQHVDHSAGELIQLVQGLLRYNPVDRLTARQA 420

Query: 421 LRHPFFTRDPLRR 434
           L+HPFFTRDPLRR
Sbjct: 421 LKHPFFTRDPLRR 433

BLAST of CmoCh18G001770 vs. NCBI nr
Match: XP_022954852.1 (serine/threonine-protein kinase AFC2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 909.8 bits (2350), Expect = 8.8e-261
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE
Sbjct: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420
           GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA
Sbjct: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420

Query: 421 LRHPFFTRDPLRRL 435
           LRHPFFTRDPLRRL
Sbjct: 421 LRHPFFTRDPLRRL 434

BLAST of CmoCh18G001770 vs. NCBI nr
Match: XP_022954851.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 905.2 bits (2338), Expect = 2.2e-259
Identity = 434/435 (99.77%), Postives = 434/435 (99.77%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE
Sbjct: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240

Query: 241 HDYK-NLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300
           HDYK NLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG
Sbjct: 241 HDYKQNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360
           WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR
Sbjct: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360

Query: 361 RGRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE 420
           RGRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE
Sbjct: 361 RGRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE 420

Query: 421 ALRHPFFTRDPLRRL 435
           ALRHPFFTRDPLRRL
Sbjct: 421 ALRHPFFTRDPLRRL 435

BLAST of CmoCh18G001770 vs. NCBI nr
Match: XP_022994548.1 (serine/threonine-protein kinase AFC2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 901.0 bits (2327), Expect = 4.1e-258
Identity = 431/434 (99.31%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE
Sbjct: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDL MVHTDLKPENVLLVSADYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLLMVHTDLKPENVLLVSADYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMME VLGPLPHEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPHEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420
           GRLDWPEGATSRDS+KAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420

Query: 421 LRHPFFTRDPLRRL 435
           LRHPFFTRDPLRRL
Sbjct: 421 LRHPFFTRDPLRRL 434

BLAST of CmoCh18G001770 vs. NCBI nr
Match: XP_023542676.1 (serine/threonine-protein kinase AFC2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 901.0 bits (2327), Expect = 4.1e-258
Identity = 430/434 (99.08%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQ+ GNMPSFASSKAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQDFGNMPSFASSKAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE
Sbjct: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMME VLGPLPHEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPHEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420
           GRLDWPEGATSRDS+KAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420

Query: 421 LRHPFFTRDPLRRL 435
           LRHPFFTRDPLRRL
Sbjct: 421 LRHPFFTRDPLRRL 434

BLAST of CmoCh18G001770 vs. NCBI nr
Match: XP_022994547.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 896.3 bits (2315), Expect = 1.0e-256
Identity = 431/435 (99.08%), Postives = 432/435 (99.31%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE
Sbjct: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDL MVHTDLKPENVLLVSADYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLLMVHTDLKPENVLLVSADYVKV 240

Query: 241 HDYK-NLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300
           HDYK NLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG
Sbjct: 241 HDYKQNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360
           WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMME VLGPLPHEMLKRVDRHAEKYVR
Sbjct: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPHEMLKRVDRHAEKYVR 360

Query: 361 RGRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE 420
           RGRLDWPEGATSRDS+KAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE
Sbjct: 361 RGRLDWPEGATSRDSIKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHE 420

Query: 421 ALRHPFFTRDPLRRL 435
           ALRHPFFTRDPLRRL
Sbjct: 421 ALRHPFFTRDPLRRL 435

BLAST of CmoCh18G001770 vs. TAIR 10
Match: AT4G24740.1 (FUS3-complementing gene 2 )

HSP 1 Score: 753.1 bits (1943), Expect = 1.3e-217
Identity = 358/428 (83.64%), Postives = 397/428 (92.76%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIGNMPSFASSKAPSDH 60
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A KAQVG+ CGQEIGN+ SFASS APSD+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 61  SSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDNE 120
           SS  L VKGVARNGSPPWREDDKDGHY+F LGD+LT RYKI+SKMGEGTFGQVLECWD E
Sbjct: 61  SSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVA+KIVRG++KYR+AAMIEIE+LQQL KHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVKV 240
           G SLYDFLRKNNYRSFPIDLVR+IG QLLECVAFMHDLRM+HTDLKPEN+LLVS+DYVK+
Sbjct: 181 GSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKI 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLGW 300
            +YK  SR  RD   +KRVPKSSAIKVIDFGSTTY+R DQ YIVSTRHYRAPEVILGLGW
Sbjct: 241 PEYKG-SRLQRDVC-YKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVRR 360
           S+PCD+WSVGCI+VELC+GEALFQTHENLEHLAMME VLGP P +MLK+VDRH+EKYVRR
Sbjct: 301 SYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAHEA 420
           GRLDWP+GATSRDS+KAV KLPRL NLIMQHVDHSAGELI++VQGLLR++P++R+TA EA
Sbjct: 361 GRLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAREA 420

Query: 421 LRHPFFTR 429
           LRHPFF R
Sbjct: 421 LRHPFFAR 425

BLAST of CmoCh18G001770 vs. TAIR 10
Match: AT3G53570.3 (FUS3-complementing gene 1 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-183
Identity = 304/430 (70.70%), Postives = 355/430 (82.56%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIGNMPSFASSKAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P +
Sbjct: 3   LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVP-N 62

Query: 61  HSSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDN 120
                +F  G+ R GSPPWR DDKDGHY+F +GD LT RY+I SKMGEGTFGQVLEC+DN
Sbjct: 63  FVYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 122

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 123 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 182

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR++HTDLKPEN+LLVS++Y+K
Sbjct: 183 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 242

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++  D NYIVSTRHYRAPEVILG+G
Sbjct: 243 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 302

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMME VLGPLP  M+ R DR +EKY R
Sbjct: 303 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 362

Query: 361 RG-RLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAH 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRY+P +R  A 
Sbjct: 363 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 422

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 423 EALNHPFFTR 431

BLAST of CmoCh18G001770 vs. TAIR 10
Match: AT3G53570.1 (FUS3-complementing gene 1 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-183
Identity = 304/430 (70.70%), Postives = 355/430 (82.56%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIGNMPSFASSKAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P +
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVP-N 76

Query: 61  HSSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDN 120
                +F  G+ R GSPPWR DDKDGHY+F +GD LT RY+I SKMGEGTFGQVLEC+DN
Sbjct: 77  FVYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR++HTDLKPEN+LLVS++Y+K
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++  D NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMME VLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

Query: 361 RG-RLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAH 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRY+P +R  A 
Sbjct: 377 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 436

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 437 EALNHPFFTR 445

BLAST of CmoCh18G001770 vs. TAIR 10
Match: AT3G53570.2 (FUS3-complementing gene 1 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-183
Identity = 304/430 (70.70%), Postives = 355/430 (82.56%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIGNMPSFASSKAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P +
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVP-N 76

Query: 61  HSSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDN 120
                +F  G+ R GSPPWR DDKDGHY+F +GD LT RY+I SKMGEGTFGQVLEC+DN
Sbjct: 77  FVYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR++HTDLKPEN+LLVS++Y+K
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++  D NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMME VLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

Query: 361 RG-RLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAH 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRY+P +R  A 
Sbjct: 377 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 436

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 437 EALNHPFFTR 445

BLAST of CmoCh18G001770 vs. TAIR 10
Match: AT3G53570.4 (FUS3-complementing gene 1 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-183
Identity = 304/430 (70.70%), Postives = 355/430 (82.56%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIGNMPSFASSKAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P +
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVP-N 76

Query: 61  HSSDPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDN 120
                +F  G+ R GSPPWR DDKDGHY+F +GD LT RY+I SKMGEGTFGQVLEC+DN
Sbjct: 77  FVYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLQKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMVHTDLKPENVLLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR++HTDLKPEN+LLVS++Y+K
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRPDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++  D NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMECVLGPLPHEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMME VLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

Query: 361 RG-RLDWPEGATSRDSMKAVQKLPRLPNLIMQHVDHSAGELIHLVQGLLRYEPADRLTAH 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRY+P +R  A 
Sbjct: 377 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 436

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 437 EALNHPFFTR 445

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P515671.8e-21683.64Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=... [more]
P515661.7e-18270.70Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=... [more]
P515681.6e-14360.83Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=... [more]
P497601.8e-9148.17Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1[more]
O354912.4e-9148.44Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1GS084.3e-261100.00serine/threonine-protein kinase AFC2-like isoform X2 OS=Cucurbita moschata OX=36... [more]
A0A6J1GS361.0e-25999.77serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita moschata OX=36... [more]
A0A6J1K5G92.0e-25899.31serine/threonine-protein kinase AFC2-like isoform X2 OS=Cucurbita maxima OX=3661... [more]
A0A6J1JW594.9e-25799.08serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1C5J15.4e-24894.92serine/threonine-protein kinase AFC2 isoform X2 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_022954852.18.8e-261100.00serine/threonine-protein kinase AFC2-like isoform X2 [Cucurbita moschata][more]
XP_022954851.12.2e-25999.77serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita moschata][more]
XP_022994548.14.1e-25899.31serine/threonine-protein kinase AFC2-like isoform X2 [Cucurbita maxima][more]
XP_023542676.14.1e-25899.08serine/threonine-protein kinase AFC2-like isoform X2 [Cucurbita pepo subsp. pepo... [more]
XP_022994547.11.0e-25699.08serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G24740.11.3e-21783.64FUS3-complementing gene 2 [more]
AT3G53570.31.2e-18370.70FUS3-complementing gene 1 [more]
AT3G53570.11.2e-18370.70FUS3-complementing gene 1 [more]
AT3G53570.21.2e-18370.70FUS3-complementing gene 1 [more]
AT3G53570.41.2e-18370.70FUS3-complementing gene 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 99..426
e-value: 2.8E-73
score: 259.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 99..426
e-value: 3.5E-48
score: 164.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 99..426
score: 36.710957
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 86..179
e-value: 1.1E-110
score: 372.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 153..426
e-value: 1.1E-110
score: 372.1
NoneNo IPR availablePANTHERPTHR45646:SF16KINASE AFC1coord: 1..433
NoneNo IPR availablePANTHERPTHR45646SERINE/THREONINE-PROTEIN KINASE DOA-RELATEDcoord: 1..433
NoneNo IPR availableCDDcd14134PKc_CLKcoord: 86..426
e-value: 0.0
score: 549.86
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 220..232
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 86..430

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh18G001770.1CmoCh18G001770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006397 mRNA processing
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity