CmoCh17G013360 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh17G013360
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGlutathione gamma-glutamylcysteinyltransferase
LocationCmo_Chr17: 10461869 .. 10464615 (-)
RNA-Seq ExpressionCmoCh17G013360
SyntenyCmoCh17G013360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGTCGCCGGATTTTATCGCAGAGTTCTTCCCTCTCCTCCGGCTATAGATTTTTCTTCTTCCGATGGAAAGGTGCTTTCAACACCTCAGATCTTCTTTCTCCGTCCCTTTTCAATTTCTTGATCATTCTCTTAATGCCTTTTTGCTTTTTTTACTGACTTTAGCTGTATGAATCTTGCAGCGTCTGTTTACTGAGGCGCTTGGAGATGGAACCATGGAAGGGTTTTTCAAGTTGATTTCTTACTACCAAACGCAATCGGAACCGGCTTTTTGTGGACTTGCCACCCTTGCCGTTGTCCTCAATGCTCTTTCTATTGATCCTGGTAGAAAATGGAAAGGTCAGTCCAAATTCGATACTTATTTTCCCTTATTAATCGAGAGCCCATTTTTCATTGGATCTGCTACTAGCAACACTAACAATCGATGAATTACAATCCCATACCAATTTTAGTCCTCAGATGTACTGTAATATCCGCTTCAGTATCTTGTATGCATACATCGACCAATGCATTGATTTTTCTTTTCAGTCATTCTAATACTATGTTGGAAGTCCCTTTTCCAGTTTGGAGAACATTCGCAATTTCAGTTCCAAACAGGATTATTTGGTGCCCTGGCTATTTTTTGCTAATTGGATAGGACAAAACTGTTCTCTGTCAATGAGCAATATTTAGACGCAAGAAAAGAGAGAAGATTGGATATACCTTCATAGCAACAGGACTCATGTAAGTTTGATGAAACGATTGTTCTTGTTTTCTTCAGGTCCGTGGAGATGGTTTGATGACACTATGCTGGACTGCTGTGAACCTTTGGCAAAGATCAAAACTGATGGTATCACATTTGGCAAGGTTGCATGTTTGGCTCGCTGCAATGGTGCTAAAGTGGTAGCTTTTAGGGCAAATGAAAGCACCATTAATGATTTTCGCAAGTATGTCGTGTCATGTTCATCTTCTGAGGACTGTCATGTGATCACTTCCTACCATAGAGGAGCTTTTAAACAGGTATATCGAAGTCTATATCTTCACCAATGGTCTTACTATATGTCCTCAAAATTAGCTGTAGTAACTGAAAGAGGTGTATAGTTTTGTAAATCTAAACCAATGAGCTGAAGTTTAATATATTATATATAAGAAAATACTAATATAATAGCATAAGTTCACCGCTAGCAGATATTGTCTTCTTTGGGCTTTCTCTTATGAGCTTAAAAATGAGTTTACTTGGGAGAGGTTTCCACGTCTTTATAACGAATACTTTGTTCTCTCACAATGGTTCAAGATGGGGCCCTCGTTCATGAGAAACAACTCTTGACATTCTGAAGATGTGGTGTATTTGATCACTTGTCATAGAAGTTAATTATAGGCTTGTCTTTAACATAATAATTGGTTTCAGACTGGAAGTGGCCATTTTTCACCAATTGGGGGTTATCATACTGGAAAAGATATGGTTCTCATCTTGGATGTTGCGCGTTTCAAGTATCCTCCACACTGGGTTCCTCTTACCCTACTGTGGGATGCCATGAACACAATTGACAGTGCAACAGGACTACCTAGAGGGTAAACCCATATTCTTATTTCTCGTGTTTATTTGTGGAGTAGCTTCTGTTCTGCAGATCCATGTTCTGAAGTTTTCTTTTTAACGCAGCTACATGATTTTATCAAAGCTTCCCAGGGGACCGTCTATTCTTTACACTCTGGTACATCCTTTTCATAAACTTCAAAGTTGTTTGATAAGTCTCTAAACTTCAAAGTGTATGTTTTTAGGTCTTCTCGTCTACGTACGTGTTAAGAATTAATATTGAACACTGGTCGTAAATTCGTTTTGTGCAGAGTTGTCAGCATGATGGTTGGAATAGCGTTATAAAATACTTAACAGAAGAAATCCCCCAACTTTTGAAAGCAGAAGATGTGAAAGATGTAAGGGGATTACTTTCTGTAGTATTTAAAATGCCACCTCCAAATCTCAGACAATTTATCAAGTGGGTTGCAGAAGTCCGGGAGAAAGAGGATGGGAATGTAAAACTAAATGCAGAGGAGAGAGGAAGACTAGCTGTCAAGGTTGTTCTCTAGAGCTTCCTTCTGTTATTTTTCTTGCTATGATTTGTTTTATGAGGTTTAAGTATGGGAACCTCAGGAAGAGATATTGGAACAACTCCGAGCGACTGAACTCTTCAAACATGTCAAACAATGGTTGGCATCTGAGACACTCTGCAAAGGCTTGACATCTTCGTTTGACAAAGATGAATTACATGCAATTGCAGCAAGTGTTTGTTGTCAAGGGGCAGAAACTTTAGGTGGGAAACGATGCTCGGCCGATCGAAGTTGCTGCAAAACCGATATCCATCTTTTGAATGGTGAAAATGAGAAGGCAGCAGTGGTGGTGGCAGGGAGCGTTGTTAGTAATGTTAGTACTAAACAAGGGGTAGATATGTTGGTACCTTTGTGTAAAACAGATGAGTGTTGTTGTGAGTGGCCATCTGTTGTTGATGTTCTAACCATATTGTTGCTATCACTGCCCCAACATATTTGGTCCACCGTTAAAGAAGAGAAGCTGTTGGCAGACATTAACACACTTGTTGCAGAAAAGAACCTTCCTGCTTTGCTTCAGGATGAGGTAATCCAGCTTTGAGCTTCTTTGTTTCCTTTCTGCTTTGGTTTGAAAAAAAAAAAGTACATCCATATTATTCCTTGCAGGTTCTGCACTTGAGACAACAAATGCACCTTCTTATGACTGATCTCGGCTCTCAATAG

mRNA sequence

ATGGCCGTCGCCGGATTTTATCGCAGAGTTCTTCCCTCTCCTCCGGCTATAGATTTTTCTTCTTCCGATGGAAAGCGTCTGTTTACTGAGGCGCTTGGAGATGGAACCATGGAAGGGTTTTTCAAGTTGATTTCTTACTACCAAACGCAATCGGAACCGGCTTTTTGTGGACTTGCCACCCTTGCCGTTGTCCTCAATGCTCTTTCTATTGATCCTGGTAGAAAATGGAAAGGTCCGTGGAGATGGTTTGATGACACTATGCTGGACTGCTGTGAACCTTTGGCAAAGATCAAAACTGATGGTATCACATTTGGCAAGGTTGCATGTTTGGCTCGCTGCAATGGTGCTAAAGTGGTAGCTTTTAGGGCAAATGAAAGCACCATTAATGATTTTCGCAAGTATGTCGTGTCATGTTCATCTTCTGAGGACTGTCATGTGATCACTTCCTACCATAGAGGAGCTTTTAAACAGACTGGAAGTGGCCATTTTTCACCAATTGGGGGTTATCATACTGGAAAAGATATGGTTCTCATCTTGGATGTTGCGCGTTTCAAGTATCCTCCACACTGGGTTCCTCTTACCCTACTGTGGGATGCCATGAACACAATTGACAGTGCAACAGGACTACCTAGAGGCTACATGATTTTATCAAAGCTTCCCAGGGGACCGTCTATTCTTTACACTCTGAGTTGTCAGCATGATGGTTGGAATAGCGTTATAAAATACTTAACAGAAGAAATCCCCCAACTTTTGAAAGCAGAAGATGTGAAAGATGTAAGGGGATTACTTTCTGTAGTATTTAAAATGCCACCTCCAAATCTCAGACAATTTATCAAGTGGGTTGCAGAAGTCCGGGAGAAAGAGGATGGGAATGTAAAACTAAATGCAGAGGAGAGAGGAAGACTAGCTGTCAAGGAAGAGATATTGGAACAACTCCGAGCGACTGAACTCTTCAAACATGTCAAACAATGGTTGGCATCTGAGACACTCTGCAAAGGCTTGACATCTTCGTTTGACAAAGATGAATTACATGCAATTGCAGCAAGTGTTTGTTGTCAAGGGGCAGAAACTTTAGGTGGGAAACGATGCTCGGCCGATCGAAGTTGCTGCAAAACCGATATCCATCTTTTGAATGGTGAAAATGAGAAGGCAGCAGTGGTGGTGGCAGGGAGCGTTGTTAGTAATGTTAGTACTAAACAAGGGGTAGATATGTTGGTACCTTTGTGTAAAACAGATGAGTGTTGTTGTGAGTGGCCATCTGTTGTTGATGTTCTAACCATATTGTTGCTATCACTGCCCCAACATATTTGGTCCACCGTTAAAGAAGAGAAGCTGTTGGCAGACATTAACACACTTGTTGCAGAAAAGAACCTTCCTGCTTTGCTTCAGGATGAGGTTCTGCACTTGAGACAACAAATGCACCTTCTTATGACTGATCTCGGCTCTCAATAG

Coding sequence (CDS)

ATGGCCGTCGCCGGATTTTATCGCAGAGTTCTTCCCTCTCCTCCGGCTATAGATTTTTCTTCTTCCGATGGAAAGCGTCTGTTTACTGAGGCGCTTGGAGATGGAACCATGGAAGGGTTTTTCAAGTTGATTTCTTACTACCAAACGCAATCGGAACCGGCTTTTTGTGGACTTGCCACCCTTGCCGTTGTCCTCAATGCTCTTTCTATTGATCCTGGTAGAAAATGGAAAGGTCCGTGGAGATGGTTTGATGACACTATGCTGGACTGCTGTGAACCTTTGGCAAAGATCAAAACTGATGGTATCACATTTGGCAAGGTTGCATGTTTGGCTCGCTGCAATGGTGCTAAAGTGGTAGCTTTTAGGGCAAATGAAAGCACCATTAATGATTTTCGCAAGTATGTCGTGTCATGTTCATCTTCTGAGGACTGTCATGTGATCACTTCCTACCATAGAGGAGCTTTTAAACAGACTGGAAGTGGCCATTTTTCACCAATTGGGGGTTATCATACTGGAAAAGATATGGTTCTCATCTTGGATGTTGCGCGTTTCAAGTATCCTCCACACTGGGTTCCTCTTACCCTACTGTGGGATGCCATGAACACAATTGACAGTGCAACAGGACTACCTAGAGGCTACATGATTTTATCAAAGCTTCCCAGGGGACCGTCTATTCTTTACACTCTGAGTTGTCAGCATGATGGTTGGAATAGCGTTATAAAATACTTAACAGAAGAAATCCCCCAACTTTTGAAAGCAGAAGATGTGAAAGATGTAAGGGGATTACTTTCTGTAGTATTTAAAATGCCACCTCCAAATCTCAGACAATTTATCAAGTGGGTTGCAGAAGTCCGGGAGAAAGAGGATGGGAATGTAAAACTAAATGCAGAGGAGAGAGGAAGACTAGCTGTCAAGGAAGAGATATTGGAACAACTCCGAGCGACTGAACTCTTCAAACATGTCAAACAATGGTTGGCATCTGAGACACTCTGCAAAGGCTTGACATCTTCGTTTGACAAAGATGAATTACATGCAATTGCAGCAAGTGTTTGTTGTCAAGGGGCAGAAACTTTAGGTGGGAAACGATGCTCGGCCGATCGAAGTTGCTGCAAAACCGATATCCATCTTTTGAATGGTGAAAATGAGAAGGCAGCAGTGGTGGTGGCAGGGAGCGTTGTTAGTAATGTTAGTACTAAACAAGGGGTAGATATGTTGGTACCTTTGTGTAAAACAGATGAGTGTTGTTGTGAGTGGCCATCTGTTGTTGATGTTCTAACCATATTGTTGCTATCACTGCCCCAACATATTTGGTCCACCGTTAAAGAAGAGAAGCTGTTGGCAGACATTAACACACTTGTTGCAGAAAAGAACCTTCCTGCTTTGCTTCAGGATGAGGTTCTGCACTTGAGACAACAAATGCACCTTCTTATGACTGATCTCGGCTCTCAATAG

Protein sequence

MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDLGSQ
Homology
BLAST of CmoCh17G013360 vs. ExPASy Swiss-Prot
Match: Q2QKL5 (Glutathione gamma-glutamylcysteinyltransferase 3 OS=Lotus japonicus OX=34305 GN=PCS3 PE=2 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 1.1e-185
Identity = 310/470 (65.96%), Postives = 373/470 (79.36%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MA AG YRRVLPSPPAIDF+S +GK++F EALG GTMEGFFKL+SYYQTQSEPA+CGLAT
Sbjct: 1   MASAGLYRRVLPSPPAIDFASPEGKKIFVEALGQGTMEGFFKLVSYYQTQSEPAYCGLAT 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           L VVLNALSIDPGRKWKGPWRWFDD+MLDCCEPL KIK  GITFGKVACLARCNGA V A
Sbjct: 61  LTVVLNALSIDPGRKWKGPWRWFDDSMLDCCEPLEKIKVQGITFGKVACLARCNGAHVEA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FR+NEST++DFR  V+SC SSED H+I SYHR   KQTG GHFSPIGGYH  +DMVLILD
Sbjct: 121 FRSNESTVSDFRDRVISCCSSEDRHLIVSYHRSGLKQTGEGHFSPIGGYHAERDMVLILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           V R+KYPPHWVPLTLLWDAMNTID ATGL RGYMI+SKL R PSILYT+SC+H+GW+SV 
Sbjct: 181 VTRYKYPPHWVPLTLLWDAMNTIDRATGLQRGYMIISKLKRAPSILYTVSCRHEGWSSVA 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           K+LTE +P LLK+ED+KD++ +LSVVFK PP  LR+FI W+AEVR +EDGN+ L+ EE+G
Sbjct: 241 KFLTENVPLLLKSEDLKDIQEVLSVVFKSPPSELREFITWIAEVRRQEDGNLTLSEEEKG 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASE-TLCKGLTSSFDKDELHAIAASVCCQGAETLG 360
           RLA+K +ILEQ+R T LFKHV  WL S+ + C+ +    D+D L  +AA VCCQGA  L 
Sbjct: 301 RLAIKADILEQIRTTTLFKHVTSWLDSQRSRCRTIAKLQDRDMLPELAAGVCCQGACLLT 360

Query: 361 GKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCK---TDECCC 420
           G      + C + D+  LN +++    +V+G+V S  S++QGVD+LVPLC+      C  
Sbjct: 361 GCCLPGGKCCSQIDVKHLNVDHKNIVTLVSGTVASGSSSEQGVDVLVPLCQMGPEGHCIG 420

Query: 421 EWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEV 467
             PS  DVLT+LLL+LP H WS +KEEKL A++ +L+  +NLP LLQ+EV
Sbjct: 421 MHPSTADVLTVLLLALPLHTWSGIKEEKLCAEVTSLLTTENLPPLLQEEV 470

BLAST of CmoCh17G013360 vs. ExPASy Swiss-Prot
Match: Q2TE74 (Glutathione gamma-glutamylcysteinyltransferase 2 OS=Lotus japonicus OX=34305 GN=PCS2 PE=2 SV=2)

HSP 1 Score: 637.1 bits (1642), Expect = 1.6e-181
Identity = 304/472 (64.41%), Postives = 372/472 (78.81%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MA    YRRVLPSPPAIDF+S +GK++F EALG GTMEGFFKLIS+YQTQSEPA+CGLAT
Sbjct: 1   MASVSLYRRVLPSPPAIDFASQEGKKIFVEALGQGTMEGFFKLISFYQTQSEPAYCGLAT 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           L+VVLNAL+IDPGRKWKGPWRWFDD+MLDCCEPL K+K  GITFGKVACLARCNGAKV A
Sbjct: 61  LSVVLNALAIDPGRKWKGPWRWFDDSMLDCCEPLEKVKVQGITFGKVACLARCNGAKVEA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FR+NEST++DFR  V+SC SSED H I SYHR   KQTG GHFSP+GGYH  +DMVLILD
Sbjct: 121 FRSNESTVSDFRNRVISCCSSEDRHAIVSYHRSGLKQTGEGHFSPLGGYHAERDMVLILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           V R+KYPPHWVP+TLLW+AMNTID ATGL RGYMI+SKL R PSILYTLSC+H+GW+SV 
Sbjct: 181 VTRYKYPPHWVPVTLLWNAMNTIDRATGLQRGYMIISKLNRAPSILYTLSCRHEGWSSVA 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           K+LTE +P LLK+ED+KD++ +LSVVFK PP  LR+FI W+AEVR +EDGN+ L+ EE+G
Sbjct: 241 KFLTENVPLLLKSEDLKDIQEVLSVVFKSPPSELREFITWIAEVRRQEDGNLTLSEEEKG 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASE-TLCKGLTSSFDKDELHAIAASVCCQGAETLG 360
           RL +K EILE++R   LFKHV  WL S+ + C  + +  DKD L  +AA VCCQGA  L 
Sbjct: 301 RLVIKAEILEKIRTAGLFKHVTSWLDSQRSRCSTIANLQDKDMLPELAARVCCQGACLLT 360

Query: 361 GKRCSADRSCCKTDIHLLNGENEK-AAVVVAGSVVSNVSTKQGVDMLVPLCK---TDECC 420
           G   S  + CC+ D+  LN +++  A   V+G+  +  S++QGVD+LVPLC+        
Sbjct: 361 GCCLSGGKCCCQIDVKHLNVDSKNLATTFVSGTFTTGSSSEQGVDVLVPLCQRGPEGHYI 420

Query: 421 CEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVL 468
              PS  DVLT+LLL+LP H WS +KEEKL A++ +LV  ++LP+LLQ+EV+
Sbjct: 421 AMHPSTADVLTVLLLALPLHTWSGIKEEKLRAEVTSLVTTEDLPSLLQEEVI 472

BLAST of CmoCh17G013360 vs. ExPASy Swiss-Prot
Match: Q2TSC7 (Glutathione gamma-glutamylcysteinyltransferase 1 OS=Lotus japonicus OX=34305 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.2e-170
Identity = 292/487 (59.96%), Postives = 377/487 (77.41%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MA+AG YRR+LPSPPA+DF+SS GK+LF EA+ +GTME F++L+SY+QTQSEPAFCGLA+
Sbjct: 1   MAMAGLYRRLLPSPPAVDFASSQGKQLFLEAVQNGTMESFYRLVSYFQTQSEPAFCGLAS 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           L++VLNAL+IDPGRKWKGPWRWFD++MLDCCEPL KIK  GI+FGK+ CLA C GAKV A
Sbjct: 61  LSMVLNALAIDPGRKWKGPWRWFDESMLDCCEPLDKIKARGISFGKLVCLAHCAGAKVEA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           F A+ S+I+ FRKYV+ CS+S+DCHVI+SYHR A KQTG+GHFSPIGGYH GKDM LILD
Sbjct: 121 FHASHSSIDHFRKYVMKCSTSDDCHVISSYHREALKQTGTGHFSPIGGYHAGKDMALILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHW+PLT LW+ MN +D +TG  RG+M++S+  R P +LYTLSC+H+ WNS+ 
Sbjct: 181 VARFKYPPHWIPLTHLWEGMNYVDESTGKTRGFMLISRPHREPGMLYTLSCKHESWNSIA 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           K+L ++IP LL +EDVKD+  +LSV+    P N  +FIKWVAE+R  EDG+  L+ EE+ 
Sbjct: 241 KFLIDDIPFLLTSEDVKDICKVLSVIVTSLPSNFEEFIKWVAEIRRGEDGSPSLSVEEKA 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLT-SSFDKDELHAIAASVCCQGAETLG 360
           RL+VKEEIL+Q++ T LFKHV  +L+    C G T +S D+D    IAASVCCQGAE LG
Sbjct: 301 RLSVKEEILKQVQRTGLFKHVASFLSHS--CSGHTPTSGDRDTFPVIAASVCCQGAEILG 360

Query: 361 GKRCSADRSCCK-TDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVP-LCKTDECCC- 420
           GK  S+   CC+ T +     E++K   +V G+VV N +T+QGVD+L+P  C    C C 
Sbjct: 361 GKISSSAEYCCRETCMKCWKAEDDKPIRMVCGTVV-NGNTEQGVDVLIPSSCGKLSCTCS 420

Query: 421 -------EWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHL 477
                  + P+  DVLT+LLLSLP   W+ + +EKLL++I+ LV+ +NLPALLQ+EVLHL
Sbjct: 421 STTKSIRKHPASTDVLTVLLLSLPTSTWAGIADEKLLSEIHDLVSIENLPALLQEEVLHL 480

BLAST of CmoCh17G013360 vs. ExPASy Swiss-Prot
Match: Q9SWW5 (Glutathione gamma-glutamylcysteinyltransferase 1 OS=Triticum aestivum OX=4565 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 5.0e-159
Identity = 274/486 (56.38%), Postives = 352/486 (72.43%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           M VA  YRRVLPSPPA++F+S++GKRLF EAL  GTMEGFF LISY+QTQSEPAFCGLA+
Sbjct: 1   MEVASLYRRVLPSPPAVEFASAEGKRLFAEALQGGTMEGFFNLISYFQTQSEPAFCGLAS 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           L+VVLNAL+IDPGR WKGPWRWFD++MLDCCEPL K+K +GITFGKV CLA C GA+V +
Sbjct: 61  LSVVLNALAIDPGRPWKGPWRWFDESMLDCCEPLHKVKAEGITFGKVVCLAHCAGARVQS 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FRA+++TI+DFR ++  C+SS+DCH+I+SYHR  FKQTG+GHFSPIGGYH  KDM LILD
Sbjct: 121 FRADQTTIHDFRAHLTRCASSQDCHLISSYHRSPFKQTGTGHFSPIGGYHAEKDMALILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPLTLLWDAMNT D ATGL RG+M++S+    PS+LYT+SC H  W S+ 
Sbjct: 181 VARFKYPPHWVPLTLLWDAMNTTDEATGLLRGFMLVSRRSSAPSLLYTVSCGHGSWKSMA 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KY  E++P LLK E + +V  LLS + +  P N    IK V EVR KE+G   L+ EE+ 
Sbjct: 241 KYCVEDVPNLLKDESLDNVTTLLSRLVESLPANAGDLIKCVIEVRRKEEGESSLSKEEKE 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RL +KE++L+Q+R T+LF+ V +    + LC   +SS D+D L  IAA+VCCQGA  L G
Sbjct: 301 RLFLKEKVLQQIRDTDLFRVVHELQYPKGLCGSCSSSSDEDSLAEIAATVCCQGAAFLSG 360

Query: 361 KRCSADRSCCK-TDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCC--- 420
              S D  CC+ T I  +    +    V++G+VVS    +Q VD+L+P   +    C   
Sbjct: 361 NLVSRDGFCCRETCIKCIEANGDGLKTVISGTVVSK-GNEQAVDLLLPTSSSKTSLCNSN 420

Query: 421 ------EWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLR 477
                 ++PS  DVLT+LLL L  + W  +K+E + A+  +LV+  NLP LL+ E+LHLR
Sbjct: 421 LKSKIVKYPSSTDVLTVLLLVLQPNTWLGIKDENVKAEFQSLVSTDNLPDLLKQEILHLR 480

BLAST of CmoCh17G013360 vs. ExPASy Swiss-Prot
Match: Q9S7Z3 (Glutathione gamma-glutamylcysteinyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=1 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.3e-156
Identity = 269/480 (56.04%), Postives = 356/480 (74.17%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MA+A  YRR LPSPPAIDFSS++GK +F EAL  GTMEGFF+LISY+QTQSEPA+CGLA+
Sbjct: 1   MAMASLYRRSLPSPPAIDFSSAEGKLIFNEALQKGTMEGFFRLISYFQTQSEPAYCGLAS 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           L+VVLNALSIDPGRKWKGPWRWFD++MLDCCEPL  +K  GI+FGKV CLA C+GAKV A
Sbjct: 61  LSVVLNALSIDPGRKWKGPWRWFDESMLDCCEPLEVVKEKGISFGKVVCLAHCSGAKVEA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FR ++STI+DFRK+VV C+SSE+CH+I++YHRG FKQTG+GHFSPIGGY+  +DM LILD
Sbjct: 121 FRTSQSTIDDFRKFVVKCTSSENCHMISTYHRGVFKQTGTGHFSPIGGYNAERDMALILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPL LLW+AM++ID +TG  RG+M++S+  R P +LYTLSC+ + W  + 
Sbjct: 181 VARFKYPPHWVPLKLLWEAMDSIDQSTGKRRGFMLISRPHREPGLLYTLSCKDESWIEIA 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYL E++P+L+ ++ V  V  ++SVVFK  P N  QFI+WVAE+R  ED N  L+AEE+ 
Sbjct: 241 KYLKEDVPRLVSSQHVDSVEKIISVVFKSLPSNFNQFIRWVAEIRITEDSNQNLSAEEKS 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RL +K+ +L+++  TELFKH+ ++L++            +D L   AA  CCQGAE L G
Sbjct: 301 RLKLKQLVLKEVHETELFKHINKFLSTVGY---------EDSLTYAAAKACCQGAEILSG 360

Query: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCC---- 420
              S +  C +T +  + G ++    VV G VV +   +Q VD+LVP  +T EC C    
Sbjct: 361 SP-SKEFCCRETCVKCIKGPDDSEGTVVTGVVVRD-GNEQKVDLLVPSTQT-ECECGPEA 420

Query: 421 EWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLL 477
            +P+  DV T LLL+LP   WS +K++ L+ ++  L++  +LP LLQ+EVLHLR+Q+ LL
Sbjct: 421 TYPAGNDVFTALLLALPPQTWSGIKDQALMHEMKQLISMASLPTLLQEEVLHLRRQLQLL 468

BLAST of CmoCh17G013360 vs. ExPASy TrEMBL
Match: A0A6J1GQY9 (Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456253 PE=4 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 2.8e-285
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT
Sbjct: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA
Sbjct: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD
Sbjct: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI
Sbjct: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG
Sbjct: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG
Sbjct: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360

Query: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS 420
           KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS
Sbjct: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS 420

Query: 421 VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL 480
           VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL
Sbjct: 421 VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL 480

Query: 481 GSQ 484
           GSQ
Sbjct: 481 GSQ 483

BLAST of CmoCh17G013360 vs. ExPASy TrEMBL
Match: A0A6J1JUJ2 (Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488492 PE=4 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.4e-276
Identity = 472/484 (97.52%), Postives = 476/484 (98.35%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT
Sbjct: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA
Sbjct: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FRANESTI+DFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD
Sbjct: 121 FRANESTIDDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWN+VI
Sbjct: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNNVI 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYLTEEIPQLLK EDVKDV  LLSVVFKMPPPNLRQFIKWVAEVRE+EDGNVKLNAEERG
Sbjct: 241 KYLTEEIPQLLKTEDVKDVGELLSVVFKMPPPNLRQFIKWVAEVREQEDGNVKLNAEERG 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIA SVC QGAETLGG
Sbjct: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIALSVCSQGAETLGG 360

Query: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQG-VDMLVPLCKTDECCCEWP 420
           KRCSADRSCCKTDIHLLNGENEKAAVVV+GSVVSNVSTKQG VDMLVPLCKTDECCCEWP
Sbjct: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVSGSVVSNVSTKQGVVDMLVPLCKTDECCCEWP 420

Query: 421 SVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTD 480
           SVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNL A LQDEVLHLRQQMHLLMTD
Sbjct: 421 SVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLSASLQDEVLHLRQQMHLLMTD 480

Query: 481 LGSQ 484
           LGSQ
Sbjct: 481 LGSQ 484

BLAST of CmoCh17G013360 vs. ExPASy TrEMBL
Match: A0A6J1GQS9 (Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456253 PE=4 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.3e-263
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 37  MEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAK 96
           MEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAK
Sbjct: 1   MEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAK 60

Query: 97  IKTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFK 156
           IKTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFK
Sbjct: 61  IKTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFK 120

Query: 157 QTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMIL 216
           QTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMIL
Sbjct: 121 QTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMIL 180

Query: 217 SKLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQ 276
           SKLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQ
Sbjct: 181 SKLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQ 240

Query: 277 FIKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTS 336
           FIKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTS
Sbjct: 241 FIKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTS 300

Query: 337 SFDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNV 396
           SFDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNV
Sbjct: 301 SFDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNV 360

Query: 397 STKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK 456
           STKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK
Sbjct: 361 STKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK 420

Query: 457 NLPALLQDEVLHLRQQMHLLMTDLGSQ 484
           NLPALLQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 421 NLPALLQDEVLHLRQQMHLLMTDLGSQ 447

BLAST of CmoCh17G013360 vs. ExPASy TrEMBL
Match: A0A6J1JSE5 (Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488492 PE=4 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 6.6e-255
Identity = 436/448 (97.32%), Postives = 440/448 (98.21%), Query Frame = 0

Query: 37  MEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAK 96
           MEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAK
Sbjct: 1   MEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAK 60

Query: 97  IKTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFK 156
           IKTDGITFGKVACLARCNGAKVVAFRANESTI+DFRKYVVSCSSSEDCHVITSYHRGAFK
Sbjct: 61  IKTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVVSCSSSEDCHVITSYHRGAFK 120

Query: 157 QTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMIL 216
           QTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMIL
Sbjct: 121 QTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMIL 180

Query: 217 SKLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQ 276
           SKLPRGPSILYTLSCQHDGWN+VIKYLTEEIPQLLK EDVKDV  LLSVVFKMPPPNLRQ
Sbjct: 181 SKLPRGPSILYTLSCQHDGWNNVIKYLTEEIPQLLKTEDVKDVGELLSVVFKMPPPNLRQ 240

Query: 277 FIKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTS 336
           FIKWVAEVRE+EDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTS
Sbjct: 241 FIKWVAEVREQEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTS 300

Query: 337 SFDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNV 396
           SFDKDELHAIA SVC QGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVV+GSVVSNV
Sbjct: 301 SFDKDELHAIALSVCSQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVSGSVVSNV 360

Query: 397 STKQG-VDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAE 456
           STKQG VDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAE
Sbjct: 361 STKQGVVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAE 420

Query: 457 KNLPALLQDEVLHLRQQMHLLMTDLGSQ 484
           KNL A LQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 421 KNLSASLQDEVLHLRQQMHLLMTDLGSQ 448

BLAST of CmoCh17G013360 vs. ExPASy TrEMBL
Match: A0A6J1DUP4 (Glutathione gamma-glutamylcysteinyltransferase OS=Momordica charantia OX=3673 GN=LOC111024196 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.6e-251
Identity = 424/488 (86.89%), Postives = 452/488 (92.62%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MAVAGFYRRVLPSPPAI+F+SS GKRLFTEALGDGTMEGFFKLISYYQTQSEPA+CGL T
Sbjct: 1   MAVAGFYRRVLPSPPAIEFASSHGKRLFTEALGDGTMEGFFKLISYYQTQSEPAYCGLGT 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           LAVVLNALSIDPGRKWKGPWRWFDD+MLDCCEPLAKIKT+GITFGKVACLARCNGAKVVA
Sbjct: 61  LAVVLNALSIDPGRKWKGPWRWFDDSMLDCCEPLAKIKTEGITFGKVACLARCNGAKVVA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FR NESTI+DFRKYVVSCSSSEDCHVITSYHRG FKQTG+GHFSPIGGYH GKDMVLILD
Sbjct: 121 FRTNESTIDDFRKYVVSCSSSEDCHVITSYHRGVFKQTGTGHFSPIGGYHAGKDMVLILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPLTLLWDAMNTID ATGLPRGYMILS+LPRGPSILYTLSCQH+GWN+VI
Sbjct: 181 VARFKYPPHWVPLTLLWDAMNTIDGATGLPRGYMILSRLPRGPSILYTLSCQHEGWNNVI 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYLTEE+P LLK EDVKDV+ LL+VVFK+PPPNLRQFIKWVAEVRE+EDGNVKL+ EERG
Sbjct: 241 KYLTEEVPLLLKTEDVKDVQELLTVVFKLPPPNLRQFIKWVAEVREQEDGNVKLSVEERG 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RLAVKEEILEQLR TELFKH+KQWLAS TLCKGL S F+KDEL  IAA+VCCQGAETL G
Sbjct: 301 RLAVKEEILEQLRETELFKHIKQWLASGTLCKGLASLFNKDELPEIAATVCCQGAETLAG 360

Query: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKT--------D 420
           K CSADR+ CKTDIHLLNGENEK AVVV+G+VVS V TKQGVDMLVPLCKT        D
Sbjct: 361 KSCSADRTFCKTDIHLLNGENEKPAVVVSGTVVSKV-TKQGVDMLVPLCKTESSQSQISD 420

Query: 421 ECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQ 480
           ECCC+WPSV+DVLTILLLSLPQHIWST+KEEKLL DI  LVAE  LPA+L++EVLHLRQQ
Sbjct: 421 ECCCDWPSVIDVLTILLLSLPQHIWSTIKEEKLLGDIKRLVAEDYLPAMLREEVLHLRQQ 480

BLAST of CmoCh17G013360 vs. NCBI nr
Match: XP_022953839.1 (glutathione gamma-glutamylcysteinyltransferase 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 990.3 bits (2559), Expect = 5.7e-285
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT
Sbjct: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA
Sbjct: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD
Sbjct: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI
Sbjct: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG
Sbjct: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG
Sbjct: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360

Query: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS 420
           KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS
Sbjct: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS 420

Query: 421 VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL 480
           VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL
Sbjct: 421 VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL 480

Query: 481 GSQ 484
           GSQ
Sbjct: 481 GSQ 483

BLAST of CmoCh17G013360 vs. NCBI nr
Match: KAG6576090.1 (Glutathione gamma-glutamylcysteinyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 970.3 bits (2507), Expect = 6.1e-279
Identity = 471/483 (97.52%), Postives = 478/483 (98.96%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPA+CGLAT
Sbjct: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAYCGLAT 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA
Sbjct: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FRANESTI+DFRKYV+SCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD
Sbjct: 121 FRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWN++I
Sbjct: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNNII 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYLTEEIPQLLK E+VKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG
Sbjct: 241 KYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFD DELHAIAASVCCQGAETLGG
Sbjct: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDTDELHAIAASVCCQGAETLGG 360

Query: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS 420
           KRC ADRSCCKTDIH L GENEKAAVVV+GSVVSNVSTKQGVDMLVPLCKTDECCCEWPS
Sbjct: 361 KRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS 420

Query: 421 VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL 480
           VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL
Sbjct: 421 VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL 480

Query: 481 GSQ 484
           GSQ
Sbjct: 481 GSQ 483

BLAST of CmoCh17G013360 vs. NCBI nr
Match: XP_023548579.1 (glutathione gamma-glutamylcysteinyltransferase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 968.0 bits (2501), Expect = 3.0e-278
Identity = 470/483 (97.31%), Postives = 478/483 (98.96%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPA+CGLAT
Sbjct: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAYCGLAT 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVV+
Sbjct: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVS 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FRANESTI+DFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD
Sbjct: 121 FRANESTIDDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWN++I
Sbjct: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNNII 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYLTEEIPQLLK EDVKDVR LLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG
Sbjct: 241 KYLTEEIPQLLKTEDVKDVRELLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG
Sbjct: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360

Query: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS 420
           ++C ADRSCCKTDIH LNGENEKAAVVV+GSVVSNVSTKQGVDMLVPLCKTDECCCEWPS
Sbjct: 361 EQCWADRSCCKTDIHHLNGENEKAAVVVSGSVVSNVSTKQGVDMLVPLCKTDECCCEWPS 420

Query: 421 VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDL 480
           VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPA LQDEVLHLRQQMHLLMTDL
Sbjct: 421 VVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPASLQDEVLHLRQQMHLLMTDL 480

Query: 481 GSQ 484
           GSQ
Sbjct: 481 GSQ 483

BLAST of CmoCh17G013360 vs. NCBI nr
Match: XP_022992015.1 (glutathione gamma-glutamylcysteinyltransferase 3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 961.4 bits (2484), Expect = 2.8e-276
Identity = 472/484 (97.52%), Postives = 476/484 (98.35%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT
Sbjct: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA
Sbjct: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FRANESTI+DFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD
Sbjct: 121 FRANESTIDDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWN+VI
Sbjct: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNNVI 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYLTEEIPQLLK EDVKDV  LLSVVFKMPPPNLRQFIKWVAEVRE+EDGNVKLNAEERG
Sbjct: 241 KYLTEEIPQLLKTEDVKDVGELLSVVFKMPPPNLRQFIKWVAEVREQEDGNVKLNAEERG 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIA SVC QGAETLGG
Sbjct: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIALSVCSQGAETLGG 360

Query: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQG-VDMLVPLCKTDECCCEWP 420
           KRCSADRSCCKTDIHLLNGENEKAAVVV+GSVVSNVSTKQG VDMLVPLCKTDECCCEWP
Sbjct: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVSGSVVSNVSTKQGVVDMLVPLCKTDECCCEWP 420

Query: 421 SVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTD 480
           SVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNL A LQDEVLHLRQQMHLLMTD
Sbjct: 421 SVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLSASLQDEVLHLRQQMHLLMTD 480

Query: 481 LGSQ 484
           LGSQ
Sbjct: 481 LGSQ 484

BLAST of CmoCh17G013360 vs. NCBI nr
Match: XP_022953840.1 (glutathione gamma-glutamylcysteinyltransferase 3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 918.3 bits (2372), Expect = 2.7e-263
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 37  MEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAK 96
           MEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAK
Sbjct: 1   MEGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAK 60

Query: 97  IKTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFK 156
           IKTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFK
Sbjct: 61  IKTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFK 120

Query: 157 QTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMIL 216
           QTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMIL
Sbjct: 121 QTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMIL 180

Query: 217 SKLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQ 276
           SKLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQ
Sbjct: 181 SKLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQ 240

Query: 277 FIKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTS 336
           FIKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTS
Sbjct: 241 FIKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTS 300

Query: 337 SFDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNV 396
           SFDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNV
Sbjct: 301 SFDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNV 360

Query: 397 STKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK 456
           STKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK
Sbjct: 361 STKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK 420

Query: 457 NLPALLQDEVLHLRQQMHLLMTDLGSQ 484
           NLPALLQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 421 NLPALLQDEVLHLRQQMHLLMTDLGSQ 447

BLAST of CmoCh17G013360 vs. TAIR 10
Match: AT5G44070.1 (phytochelatin synthase 1 (PCS1) )

HSP 1 Score: 553.5 bits (1425), Expect = 1.7e-157
Identity = 269/480 (56.04%), Postives = 356/480 (74.17%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           MA+A  YRR LPSPPAIDFSS++GK +F EAL  GTMEGFF+LISY+QTQSEPA+CGLA+
Sbjct: 1   MAMASLYRRSLPSPPAIDFSSAEGKLIFNEALQKGTMEGFFRLISYFQTQSEPAYCGLAS 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           L+VVLNALSIDPGRKWKGPWRWFD++MLDCCEPL  +K  GI+FGKV CLA C+GAKV A
Sbjct: 61  LSVVLNALSIDPGRKWKGPWRWFDESMLDCCEPLEVVKEKGISFGKVVCLAHCSGAKVEA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FR ++STI+DFRK+VV C+SSE+CH+I++YHRG FKQTG+GHFSPIGGY+  +DM LILD
Sbjct: 121 FRTSQSTIDDFRKFVVKCTSSENCHMISTYHRGVFKQTGTGHFSPIGGYNAERDMALILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPL LLW+AM++ID +TG  RG+M++S+  R P +LYTLSC+ + W  + 
Sbjct: 181 VARFKYPPHWVPLKLLWEAMDSIDQSTGKRRGFMLISRPHREPGLLYTLSCKDESWIEIA 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYL E++P+L+ ++ V  V  ++SVVFK  P N  QFI+WVAE+R  ED N  L+AEE+ 
Sbjct: 241 KYLKEDVPRLVSSQHVDSVEKIISVVFKSLPSNFNQFIRWVAEIRITEDSNQNLSAEEKS 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RL +K+ +L+++  TELFKH+ ++L++            +D L   AA  CCQGAE L G
Sbjct: 301 RLKLKQLVLKEVHETELFKHINKFLSTVGY---------EDSLTYAAAKACCQGAEILSG 360

Query: 361 KRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCC---- 420
              S +  C +T +  + G ++    VV G VV +   +Q VD+LVP  +T EC C    
Sbjct: 361 SP-SKEFCCRETCVKCIKGPDDSEGTVVTGVVVRD-GNEQKVDLLVPSTQT-ECECGPEA 420

Query: 421 EWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLL 477
            +P+  DV T LLL+LP   WS +K++ L+ ++  L++  +LP LLQ+EVLHLR+Q+ LL
Sbjct: 421 TYPAGNDVFTALLLALPPQTWSGIKDQALMHEMKQLISMASLPTLLQEEVLHLRRQLQLL 468

BLAST of CmoCh17G013360 vs. TAIR 10
Match: AT1G03980.1 (phytochelatin synthase 2 )

HSP 1 Score: 512.7 bits (1319), Expect = 3.2e-145
Identity = 256/477 (53.67%), Postives = 336/477 (70.44%), Query Frame = 0

Query: 1   MAVAGFYRRVLPSPPAIDFSSSDGKRLFTEALGDGTMEGFFKLISYYQTQSEPAFCGLAT 60
           M++A  YRR L SPPAIDF+S +GK++F EAL  GTMEGFF LISY+QTQSEPAFCGLA+
Sbjct: 1   MSMASLYRRSL-SPPAIDFASFEGKQIFNEALQKGTMEGFFGLISYFQTQSEPAFCGLAS 60

Query: 61  LAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVA 120
           L++VLN+LSIDPGRKWKGPWRWFD++ML+CCEPL  +K  GI+FGKV CLA  +GAKV A
Sbjct: 61  LSMVLNSLSIDPGRKWKGPWRWFDESMLECCEPLEIVKDKGISFGKVVCLAHSSGAKVEA 120

Query: 121 FRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILD 180
           FR N+STI+DFRKYVV CS+S++CH+I++YHR   KQTG+GHFSPIGGY+  +DM LILD
Sbjct: 121 FRTNQSTIDDFRKYVVKCSTSDNCHMISTYHRQVLKQTGTGHFSPIGGYNAERDMALILD 180

Query: 181 VARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNSVI 240
           VARFKYPPHWVPL LLWDAM++ID +TG  RG+M++S+  R P +LYTLSC+ + W S+ 
Sbjct: 181 VARFKYPPHWVPLKLLWDAMDSIDQSTGRRRGFMLISRPHREPGLLYTLSCKDESWISIA 240

Query: 241 KYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERG 300
           KYL E++P+L+ ++ V  +  +L VVFK  P N  QFIKW+AE+R  ED N  L++EE+ 
Sbjct: 241 KYLKEDVPRLVSSQHVDTIERILYVVFKSLPANFNQFIKWMAEIRRTEDVNQNLSSEEKS 300

Query: 301 RLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDKDELHAIAASVCCQGAETLGG 360
           RL +K+E+L+Q++ T+LFKHV ++L          SS  +D L  +AA V C G E L G
Sbjct: 301 RLKLKQELLKQVQETKLFKHVDKFL----------SSVYEDNLPYVAAKVYCDGDEILSG 360

Query: 361 KRCSADRSCCK-TDIHLLNGENEKAAVVVAGSVVSNVSTKQGVDMLVPLCKTDECCCEWP 420
               +D SCCK T +  + G  E+   VVA                            +P
Sbjct: 361 --YESDESCCKETCVKCIKGLGEEKVTVVA----------------------------YP 420

Query: 421 SVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLL 477
           S  DV T LLL+LP   WS +K++ LL ++  L++  + P LLQ EVLHLR+Q+ +L
Sbjct: 421 SGNDVFTALLLALPPQTWSGIKDQSLLQEMKQLISMVSHPTLLQQEVLHLRRQLEML 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2QKL51.1e-18565.96Glutathione gamma-glutamylcysteinyltransferase 3 OS=Lotus japonicus OX=34305 GN=... [more]
Q2TE741.6e-18164.41Glutathione gamma-glutamylcysteinyltransferase 2 OS=Lotus japonicus OX=34305 GN=... [more]
Q2TSC72.2e-17059.96Glutathione gamma-glutamylcysteinyltransferase 1 OS=Lotus japonicus OX=34305 GN=... [more]
Q9SWW55.0e-15956.38Glutathione gamma-glutamylcysteinyltransferase 1 OS=Triticum aestivum OX=4565 GN... [more]
Q9S7Z32.3e-15656.04Glutathione gamma-glutamylcysteinyltransferase 1 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1GQY92.8e-285100.00Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JUJ21.4e-27697.52Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GQS91.3e-263100.00Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JSE56.6e-25597.32Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1DUP47.6e-25186.89Glutathione gamma-glutamylcysteinyltransferase OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
XP_022953839.15.7e-285100.00glutathione gamma-glutamylcysteinyltransferase 3-like isoform X1 [Cucurbita mosc... [more]
KAG6576090.16.1e-27997.52Glutathione gamma-glutamylcysteinyltransferase 3, partial [Cucurbita argyrosperm... [more]
XP_023548579.13.0e-27897.31glutathione gamma-glutamylcysteinyltransferase 3-like [Cucurbita pepo subsp. pep... [more]
XP_022992015.12.8e-27697.52glutathione gamma-glutamylcysteinyltransferase 3-like isoform X1 [Cucurbita maxi... [more]
XP_022953840.12.7e-263100.00glutathione gamma-glutamylcysteinyltransferase 3-like isoform X2 [Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
AT5G44070.11.7e-15756.04phytochelatin synthase 1 (PCS1) [more]
AT1G03980.13.2e-14553.67phytochelatin synthase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038156Phytochelatin synthase, N-terminal domain superfamilyGENE3D3.90.70.30coord: 16..219
e-value: 1.3E-91
score: 307.5
IPR015407Phytochelatin synthase, C-terminalPFAMPF09328Phytochelatin_Ccoord: 231..473
e-value: 2.5E-88
score: 295.8
IPR007719Phytochelatin synthase, N-terminal catalytic domainPFAMPF05023Phytochelatincoord: 6..213
e-value: 3.4E-86
score: 287.8
IPR007719Phytochelatin synthase, N-terminal catalytic domainPROSITEPS51443PCScoord: 1..221
score: 49.652065
NoneNo IPR availablePANTHERPTHR33447:SF10GLUTATHIONE GAMMA-GLUTAMYLCYSTEINYLTRANSFERASEcoord: 5..478
IPR040409Phytochelatin synthasePANTHERPTHR33447GLUTATHIONE GAMMA-GLUTAMYLCYSTEINYLTRANSFERASEcoord: 5..478
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 13..217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh17G013360.1CmoCh17G013360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098849 cellular detoxification of cadmium ion
biological_process GO:0010273 detoxification of copper ion
biological_process GO:0046938 phytochelatin biosynthetic process
biological_process GO:0010038 response to metal ion
molecular_function GO:0016756 glutathione gamma-glutamylcysteinyltransferase activity
molecular_function GO:0046872 metal ion binding