CmoCh17G012660 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh17G012660
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Asparagine--tRNA ligase
Location	Cmo_Chr17: 9958465 .. 9962087 (+)
RNA-Seq Expression	CmoCh17G012660
Synteny	CmoCh17G012660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCCAACAACCTACAGTCTTCACCCCGGTGACCTTCTCCAAGTACACCAAGCGGGTTCTTCTCAGAACTGTGTTCGAGAGTGGCGATGGCGGTCTGGGGTTTGTGGATCGGAAATTGGTGGTCGGAGGATGGGTGAAGTCCTCCAAGGAAGTCATTGGAGATGCTGTTCGGCCGCTGGTCGATGCCAGTGTTAAGGCGGCGGGTGATGTCCTGCCGCTGAAGGCTAAGGATGCGAGCTGTATTGAGATTGTACAGTCGCGGATTCCGATTGTTAGGTCTTTGCTTAGGATGCTGTATGGAAATAATTTACACGGCGGTGAGAAGTTGGAGACGAATGTTCATGAGCCGCCTCTTCCTTCCACTGCTTTTCTGGTCGTTAATGATGGCTCTTGTGTTGCAGACCTTCAGGTTCCTCTAATTTATGCACGACAACTGTTTGTTATTTTGTTATGCGTACAGGACTTGACTAACTAAGAAACTTTCAGGTTGTTGTGGAGTCCTCGTTACATCAGCCTATCCATCTGATGCCATCGGGAACTTGCATTTTGGTGGAAGGCATATTGAAGCGGATACCAGTACCTGGGAAACATACTGTCCGGCTTGAAGTGGAGAAAATCCTTCATGTAGGGAAGGTTGAAAAGGAAAAGTATCCATTATCACAGAAAAGGATTCCAATGGATGTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACTTCTACGGTAATATGCACAAGCTTCAACTATACATTGCATTTAGTTCCTGTTTTTTGGTTCTGTCTGCATGCCTATCATCAGAGCGCCTATTAGTCTTAAAGATCGACATCGGTTGGAGAGGGAAACGAGCCTATTAGTCTTAAGATCAACATCGGTTGGAGAGGGAAACGAGCCTATTAGTCTTAAGATCGACATCGGTTGGAGAGGGAAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCCTAACAGATGCATTTTAAAAACCTTGAGGGGAAACCTGAAAGGAAAAGCCCAAAGAAGGCAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAAATGATACTAGAGCCAGACACTGAGCGGTGTGCCAGCGAGGACGTTGCCTCAAAGGGGGTGGACACTAGGCCGTGTGCCAGTAAGGACACTGGGCACCGAAGGGGGTGAATTGTGAGATCCCACATCAATTGGAGAGAGGAATGAGTGCCGGCGAGGACGAATGGCCTCGAAGGGGGTGGATTGTGAGATCTCACATCGGTTAGAGAGGGGAACGAAACATTCTTTATAAGAGCGTGGAAACCTCTTTTTAGCAGATGCGTTTTAAAAACCTTGAGAGGAAGCCTGAAAGGGAAAGCCGAAAGAGGACAATATCTGCTAGTGGTGGACTTGGGCTATTACAACGGTAATATGCACAAGCTTCAATTATACATTGCCAATGGAGATGTATTCCTTACTTATAAACCCACGATCATCCCTTTAATTAGCTAATGTGGGACTCTCTCCCAACAATCCTCAACAGAGCTCGGGTGACAAAACGTGAGAAGAGAGTTTTGTACCTTTGATCAGAACAATTGGATCTTGATCTTCATTGATACTCTTTACTTTATGCAGGTAGCATCTGTTATGAGAATTCGTAATGCCTTGACTTTTGCAACTCACACGTTCTTCCAAAATCATAGCTTTCTTTACGTGCAATTACCCACTATCACGACCACAGACAGTGAGGGATTCAGTGAAAAGTTCCACGTTACAACACTTGCGACTAAATTAGCAGGTAAGAGAGTAGAACCAACAGAAGATAAAGAAACTGATGGTGTAAGTCTTGAAACTGTTAAGGCTGCTGTAATGGAGAAAAGCAATCTCGTCAAAGAGCTTGAGAGAAGCGAAGGCAACAAAGAAGCCTTAGCTGCTGCAGTTCAGGATTTAAAGAGAACAAATGAACTGGCATTACAATTAGAAGCCAGAGGAAAATCAAAACCCGAATCGAGCAAAGATAAAGTTAACTTCCATGAAAATTTCTTCCCACAAGAAACTTATCTAACAGTTTCTGGACAACTCCATTTGGAGACCTATGCTTGCGCCCTTGGCAATGTTTATTCGTGTGGACCGAGGTTTCGAGCAGATAAAATCGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAAGTTGAAATTGCTTTTGCTGAACTCGAGGTACGAATTTATGATCTTTTTACTTATTCCTTCAGATTGCTTATTATTTGACATCTAAATTATGGATTTAAGTTTCCATGTTGATTTAGCCATTAAAATCTTAATGTTATTTTCTGTGATTAGTGGTTGTTTATAATATCATTGCACCACTGAATGAAGAAGTCCATCTTTTAACTTACATTTTGTATCATCATTGGGACTCTAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTATTGGACCACTGTGTGGATGATATGAAATTTGTTGAAAAACGAATTGACAAGACGAGCATTCCACGCCTTGAATCGTTTATATCATGTTCGTTCGAGAAGGTCTCTTACACTGCGGCAATAGAAAGTCTGGAAAAGGTGAAATCCTGATTATCACTTGTATCATTTGACTCAGTTTTTCATTTCTACCCCTGCCCACATGAAATATTGAAAGGAAAAAAAAATCTTCATTGTCAGGCAACACCCGATCAAAAACATCAACACTTGAAGAGCCTTAAATTTGGTGCTGCTTTAACTGCTGATCATCTCAGGTATGCAAAATGTGCTATTACAGTCCATAAGCAAAATGAGTTCTTTATTTTTCATTTTTTTCTTCATTTTACATGAAACAGTTATCTGGCTGATACCATCTATAAAAAGCCTGTGATAGTATACAACTACCCAGAAGGAACCAAGCCATTTAATGTGCGCCTGAATGATGATGGGAAAACTGTGGCGGCATTTGATGTAGTTCTTCCAAAGGTTAGCACATGGGCATGTATATCGACTCTTGGGAGTATTTATTTTTGGTTGGATCATCCTGTATTGAATGAAAAATTGTTTCTTAAACTGGCACCCAACTGTTTGTAGGGTGGAGTAGTAATTTCTGGCAGCCAAAAGGAGGAGCGTTTAGATGTATTGATGAGAAGGTATGATATTTTACTATACATGTGTCATCGTTTTTACTTGCAATGTCGTCGTCAGCCGTACAAGGGTGCCTTGTCTTTACTTGAAAAATAGCACATGACTTGAGTATAAATCGTTAAAACTATGATAGAGGTAGTTTCCAGTCTGGACAAAATTGGATGTGTAGTACTTCACTACACACGACCCACACGACCCACATGTTCTAATTTTACTAGTTTTGCGACACTCCGAAAGTTTTCTAAACGCTCATGAAAAAAAAATGGGAGTTATTGATATCTGTTTTAATATGGTTGTTGGCAGGATGGGGGAGATGGGGCTGCCAAGGGAGCAGTACGAGTGGTACTTGGATATCCATAGGCATGGAGCAGTGAAGCACTCCGGTTTCAGTTTGGCATTCGATCGTTTCCTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGCCAGCGATTAA

mRNA sequence

ATGGCTGCCCAACAACCTACAGTCTTCACCCCGGTGACCTTCTCCAAGTACACCAAGCGGGTTCTTCTCAGAACTGTGTTCGAGAGTGGCGATGGCGGTCTGGGGTTTGTGGATCGGAAATTGGTGGTCGGAGGATGGGTGAAGTCCTCCAAGGAAGTCATTGGAGATGCTGTTCGGCCGCTGGTCGATGCCAGTGTTAAGGCGGCGGGTGATGTCCTGCCGCTGAAGGCTAAGGATGCGAGCTGTATTGAGATTGTACAGTCGCGGATTCCGATTGTTAGGTCTTTGCTTAGGATGCTGTATGGAAATAATTTACACGGCGGTGAGAAGTTGGAGACGAATGTTCATGAGCCGCCTCTTCCTTCCACTGCTTTTCTGGTCGTTAATGATGGCTCTTGTGTTGCAGACCTTCAGGTTGTTGTGGAGTCCTCGTTACATCAGCCTATCCATCTGATGCCATCGGGAACTTGCATTTTGGTGGAAGGCATATTGAAGCGGATACCAGTACCTGGGAAACATACTGTCCGGCTTGAAGTGGAGAAAATCCTTCATGTAGGGAAGGTTGAAAAGGAAAAGTATCCATTATCACAGAAAAGGATTCCAATGGATGTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACTTCTACGGTAGCATCTGTTATGAGAATTCGTAATGCCTTGACTTTTGCAACTCACACGTTCTTCCAAAATCATAGCTTTCTTTACGTGCAATTACCCACTATCACGACCACAGACAGTGAGGGATTCAGTGAAAAGTTCCACGTTACAACACTTGCGACTAAATTAGCAGGTAAGAGAGTAGAACCAACAGAAGATAAAGAAACTGATGGTGTAAGTCTTGAAACTGTTAAGGCTGCTGTAATGGAGAAAAGCAATCTCGTCAAAGAGCTTGAGAGAAGCGAAGGCAACAAAGAAGCCTTAGCTGCTGCAGTTCAGGATTTAAAGAGAACAAATGAACTGGCATTACAATTAGAAGCCAGAGGAAAATCAAAACCCGAATCGAGCAAAGATAAAGTTAACTTCCATGAAAATTTCTTCCCACAAGAAACTTATCTAACAGTTTCTGGACAACTCCATTTGGAGACCTATGCTTGCGCCCTTGGCAATGTTTATTCGTGTGGACCGAGGTTTCGAGCAGATAAAATCGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAAGTTGAAATTGCTTTTGCTGAACTCGAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTATTGGACCACTGTGTGGATGATATGAAATTTGTTGAAAAACGAATTGACAAGACGAGCATTCCACGCCTTGAATCGTTTATATCATGTTCGTTCGAGAAGGTCTCTTACACTGCGGCAATAGAAAGTCTGGAAAAGGCAACACCCGATCAAAAACATCAACACTTGAAGAGCCTTAAATTTGGTGCTGCTTTAACTGCTGATCATCTCAGTTATCTGGCTGATACCATCTATAAAAAGCCTGTGATAGTATACAACTACCCAGAAGGAACCAAGCCATTTAATGTGCGCCTGAATGATGATGGGAAAACTGTGGCGGCATTTGATGTAGTTCTTCCAAAGGGTGGAGTAGTAATTTCTGGCAGCCAAAAGGAGGAGCGTTTAGATGTATTGATGAGAAGGATGGGGGAGATGGGGCTGCCAAGGGAGCAGTACGAGTGGTACTTGGATATCCATAGGCATGGAGCAGTGAAGCACTCCGGTTTCAGTTTGGCATTCGATCGTTTCCTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGCCAGCGATTAA

Coding sequence (CDS)

ATGGCTGCCCAACAACCTACAGTCTTCACCCCGGTGACCTTCTCCAAGTACACCAAGCGGGTTCTTCTCAGAACTGTGTTCGAGAGTGGCGATGGCGGTCTGGGGTTTGTGGATCGGAAATTGGTGGTCGGAGGATGGGTGAAGTCCTCCAAGGAAGTCATTGGAGATGCTGTTCGGCCGCTGGTCGATGCCAGTGTTAAGGCGGCGGGTGATGTCCTGCCGCTGAAGGCTAAGGATGCGAGCTGTATTGAGATTGTACAGTCGCGGATTCCGATTGTTAGGTCTTTGCTTAGGATGCTGTATGGAAATAATTTACACGGCGGTGAGAAGTTGGAGACGAATGTTCATGAGCCGCCTCTTCCTTCCACTGCTTTTCTGGTCGTTAATGATGGCTCTTGTGTTGCAGACCTTCAGGTTGTTGTGGAGTCCTCGTTACATCAGCCTATCCATCTGATGCCATCGGGAACTTGCATTTTGGTGGAAGGCATATTGAAGCGGATACCAGTACCTGGGAAACATACTGTCCGGCTTGAAGTGGAGAAAATCCTTCATGTAGGGAAGGTTGAAAAGGAAAAGTATCCATTATCACAGAAAAGGATTCCAATGGATGTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACTTCTACGGTAGCATCTGTTATGAGAATTCGTAATGCCTTGACTTTTGCAACTCACACGTTCTTCCAAAATCATAGCTTTCTTTACGTGCAATTACCCACTATCACGACCACAGACAGTGAGGGATTCAGTGAAAAGTTCCACGTTACAACACTTGCGACTAAATTAGCAGGTAAGAGAGTAGAACCAACAGAAGATAAAGAAACTGATGGTGTAAGTCTTGAAACTGTTAAGGCTGCTGTAATGGAGAAAAGCAATCTCGTCAAAGAGCTTGAGAGAAGCGAAGGCAACAAAGAAGCCTTAGCTGCTGCAGTTCAGGATTTAAAGAGAACAAATGAACTGGCATTACAATTAGAAGCCAGAGGAAAATCAAAACCCGAATCGAGCAAAGATAAAGTTAACTTCCATGAAAATTTCTTCCCACAAGAAACTTATCTAACAGTTTCTGGACAACTCCATTTGGAGACCTATGCTTGCGCCCTTGGCAATGTTTATTCGTGTGGACCGAGGTTTCGAGCAGATAAAATCGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAAGTTGAAATTGCTTTTGCTGAACTCGAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTATTGGACCACTGTGTGGATGATATGAAATTTGTTGAAAAACGAATTGACAAGACGAGCATTCCACGCCTTGAATCGTTTATATCATGTTCGTTCGAGAAGGTCTCTTACACTGCGGCAATAGAAAGTCTGGAAAAGGCAACACCCGATCAAAAACATCAACACTTGAAGAGCCTTAAATTTGGTGCTGCTTTAACTGCTGATCATCTCAGTTATCTGGCTGATACCATCTATAAAAAGCCTGTGATAGTATACAACTACCCAGAAGGAACCAAGCCATTTAATGTGCGCCTGAATGATGATGGGAAAACTGTGGCGGCATTTGATGTAGTTCTTCCAAAGGGTGGAGTAGTAATTTCTGGCAGCCAAAAGGAGGAGCGTTTAGATGTATTGATGAGAAGGATGGGGGAGATGGGGCTGCCAAGGGAGCAGTACGAGTGGTACTTGGATATCCATAGGCATGGAGCAGTGAAGCACTCCGGTTTCAGTTTGGCATTCGATCGTTTCCTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGCCAGCGATTAA

Protein sequence

MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRPLVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPLPSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVEKILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHSFLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSNLVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADDLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQKHQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLFTTGLTDIKDVIPFPRSFGKASD

Homology

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BLAST of CmoCh17G012660 vs. ExPASy Swiss-Prot
Match: Q9SW95 (Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 PE=1 SV=2)

HSP 1 Score: 577.0 bits (1486), Expect = 2.5e-163
Identity = 311/625 (49.76%), Postives = 425/625 (68.00%), Query Frame = 0


Query: 11  PVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDA---VRPLVDASVK 70
           P+T SKY+KRV L+T+ +  D G G   +++V+GGWVKS++ V  ++     P+V A   
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  AAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNN----LHGGEKLETNVHEPPLPST 130
           ++G        +  C EI+QS++ I +    +L G      +    +L      PP    
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEYV 139

Query: 131 AFLVVNDGSCVADLQVVVESSLH--QPIHLMPSGTCILVEGILKRIPV--PGKHTVRLEV 190
            + +++DGS ++ LQVVV+S+L       LM  GTCI+ EG+L R+P+    KH + LE 
Sbjct: 140 FYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVL-RLPLAASAKHVIELEA 199

Query: 191 EKILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNH 250
           EK+LHVG V+ EKYPLS+K++P+ +LR FS+FRPRT+TV SV R+ +ALT A+HTF Q H
Sbjct: 200 EKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYH 259

Query: 251 SFLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKS 310
            F YVQ+P ITTT   GF E F VTTL  K   K  +    +E DG S++TVKA + EK+
Sbjct: 260 GFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVIKEKT 319

Query: 311 NLVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPES--SKDKVNFHENFFPQE 370
            L+  L+RS+ N+E + AAV DLK+TN+LA Q+E + KSK  +    +K++F ++FF ++
Sbjct: 320 RLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFGRD 379

Query: 371 TYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNC 430
           TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL+DAM+C
Sbjct: 380 TYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAMDC 439

Query: 431 ADDLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATP 490
           AD+  KFL K+VL++  +DMKF+ KR+DKT   RLE+  S S  + SYT  I  L+KAT 
Sbjct: 440 ADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKATT 499

Query: 491 DQKHQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFD 550
            +        ++G ALT +HLSYL D IYK PVIV+ YP+  K F VRLNDD KTVAAFD
Sbjct: 500 TKFE---TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAFD 559

Query: 551 VVLPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRF 610
           +V+PK GVVI+GSQ EER ++L  R+GE G  RE++EWYLD+ RHG VKHSG SL+ ++ 
Sbjct: 560 LVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQM 619

Query: 611 LLFTTGLTDIKDVIPFPRSFGKASD 623
           LL+ TGL DIKD IPFPRS+GKA++
Sbjct: 620 LLYATGLPDIKDAIPFPRSWGKANN 638

[Close]

BLAST of CmoCh17G012660 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 4.4e-131
Identity = 253/611 (41.41%), Postives = 370/611 (60.56%), Query Frame = 0


Query: 15  SKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRPLVDASVKAAGDVLP 74
           ++++ RVL+RT+ +  DGG G   + + +GGWVKS ++                      
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD---------------------- 86

Query: 75  LKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPLPSTAFLVVNDGSCV 134
                                            G++           + +FL VNDGSC 
Sbjct: 87  --------------------------------QGKR-----------TFSFLAVNDGSCP 146

Query: 135 ADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHT---VRLEVEKILHVGKVEKE 194
           A+LQV+V+ SL+   +L+ +GTC+ V+G+LK +P  GK T   + L V K++ VG V+  
Sbjct: 147 ANLQVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDAS 206

Query: 195 KYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHSFLYVQLPTITT 254
           KYPL + ++ ++ LR   + R RT+++++V RIRNAL FATH+FFQ HSFLY+  P ITT
Sbjct: 207 KYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITT 266

Query: 255 TDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSNLVKELERSEGN 314
           +D EG  E F  TTL         +  ++       +E  +  V+E+ N+V EL+ ++ +
Sbjct: 267 SDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKAS 326

Query: 315 KEALAAAVQDLKRTNELALQLEARGKSKPESSK--DKVNFHENFFPQETYLTVSGQLHLE 374
           KEA+ AAV +LK   E    ++ R + +P   K    +++ ++FF ++ +LTVSGQL +E
Sbjct: 327 KEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVE 386

Query: 375 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADDLVKFLSKWV 434
           TYACAL NVY+ GP FRA+   +++H AE WMVE EIAFA+LED MNCA+  VK++  W+
Sbjct: 387 TYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWL 446

Query: 435 LDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQKHQHLKSLKF 494
           L+ C  DM+ + K  D   I RL+   S  F +++YT AIE LE+A    K +   ++++
Sbjct: 447 LEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGK-EFDNNVEW 506

Query: 495 GAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 554
           G  L ++H  YL + +++KP+IVYNYP+G K F +RLNDD KTVAA DV++PK G +I G
Sbjct: 507 GIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGG 566

Query: 555 SQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLFTTGLTDIKD 614
           SQ+EER DV+ +R+ EMGLP E YEWYLD+ R+G VKH GF L F+R +LF TGL +I+D
Sbjct: 567 SQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRD 570

Query: 615 VIPFPRSFGKA 621
           VIPFPR  GKA
Sbjct: 627 VIPFPRYPGKA 570

[Close]

BLAST of CmoCh17G012660 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.4e-124
Identity = 234/503 (46.52%), Postives = 330/503 (65.61%), Query Frame = 0


Query: 124 AFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPG---KHTVRLEVE 183
           AFL VNDGSC A+LQV+V+SSL+    L+ +GTC+ V+G+LK IP  G   K ++ L VE
Sbjct: 68  AFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVE 127

Query: 184 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 243
            ++ VG V+   YPL + ++  + LR   + R RT+ +++V RIRNAL FATH+FFQ HS
Sbjct: 128 TVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHS 187

Query: 244 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 303
           FLY+  P ITT+D EG  E F VTTL         +  ++       +E  +  V E+  
Sbjct: 188 FLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGE 247

Query: 304 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKD--KVNFHENFFPQET 363
            V +L+ ++ +KE + A+V  L         +E R + KP   K+  K+++  +FF ++ 
Sbjct: 248 AVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQA 307

Query: 364 YLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCA 423
           +LTVSGQL +ETYACAL +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA
Sbjct: 308 FLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCA 367

Query: 424 DDLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPD 483
           +  VK++ KW++D C DDM+ ++K +D+    RL      SF++V+YT AIE LEKA   
Sbjct: 368 EAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQ 427

Query: 484 QKHQHLKSLKFGAALTADHLSYLADTIY-KKPVIVYNYPEGTKPFNVRLNDDGKTVAAFD 543
            K      +++G  L ++H  YL +  + +KP+IVYNYP+G K F +RLNDD KTVAA D
Sbjct: 428 GKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMD 487

Query: 544 VVLPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRF 603
           V++PK G +I GSQ+EER DV+ +R+ EMGLP E YEWYLD+ R+G VKH GF L F+R 
Sbjct: 488 VLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERM 547

Query: 604 LLFTTGLTDIKDVIPFPRSFGKA 621
           + F TG+ +I+DVIPFPR  GKA
Sbjct: 548 IQFATGIDNIRDVIPFPRYPGKA 569

[Close]

BLAST of CmoCh17G012660 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 5.6e-94
Identity = 197/500 (39.40%), Postives = 288/500 (57.60%), Query Frame = 0


Query: 125 FLVVNDGSCVADLQVVVESSL---HQPIHLMPSGTCILVEGILKRIPVPGK-HTVRLEVE 184
           F+ +NDGS +++ Q++    +    Q I+ + +G  +   G +  +  PGK   + ++  
Sbjct: 40  FIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVSVSAIGTI--VESPGKEQNLEMQAT 99

Query: 185 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 244
            I  +GK + E Y L +KR   + LR  ++ RPRT+T+ +V R+RNAL FATH FFQ   
Sbjct: 100 AITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRG 159

Query: 245 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 304
           FLY+  P IT +D EG  + F VTTL                                  
Sbjct: 160 FLYIHTPIITGSDCEGAGKMFQVTTL---------------------------------- 219

Query: 305 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 364
                   + N  A         RT E                  +V++ ++FF + TYL
Sbjct: 220 --------DQNNPA---------RTPE-----------------GRVDYTQDFFGKPTYL 279

Query: 365 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 424
           TVSGQL+ E YACAL +VY+ GP FRA+   +++H AE WM+E E+AFA+L D M+CA+D
Sbjct: 280 TVSGQLNGEIYACALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAED 339

Query: 425 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 484
            +K++ K+VLD+C +DM+F  K +    I RLE  I+ SFE+ SYT A+  LEKA  D+K
Sbjct: 340 YLKYILKYVLDNCQEDMEFFNKHVATDLISRLEHVINTSFERASYTYAVRILEKA--DKK 399

Query: 485 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 544
            ++   +K+G  L ++H  +LA+  + KPVI+ +YP+  K F +R N+D KTVAA DV++
Sbjct: 400 FEY--PVKWGLDLQSEHERFLAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMDVLV 459

Query: 545 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 604
           PK G +I GSQ+EERL VL  ++ E  LP E+Y WYL++ + G+V HSGF   F+R + F
Sbjct: 460 PKVGEIIGGSQREERLSVLESKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQF 465

Query: 605 TTGLTDIKDVIPFPRSFGKA 621
           TTG+ +I+DVIPFPR  GKA
Sbjct: 520 TTGMENIRDVIPFPRHPGKA 465

[Close]

BLAST of CmoCh17G012660 vs. ExPASy Swiss-Prot
Match: P52276 (Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=asnS PE=3 SV=2)

HSP 1 Score: 343.6 bits (880), Expect = 4.7e-93
Identity = 196/501 (39.12%), Postives = 287/501 (57.29%), Query Frame = 0


Query: 120 LPSTAFLVVNDGSCVADLQVVVESSL---HQPIHLMPSGTCILVEGILKRIPVPGK-HTV 179
           L   +F+ VNDGS +A+LQVV++ SL    + +  + +G  ++VEG  K  P PGK   V
Sbjct: 31  LKDFSFVEVNDGSSLANLQVVLDGSLADYDRLLSQLQTGAALVVEG--KLAPSPGKGQRV 90

Query: 180 RLEVEKILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTF 239
            L+  K+  +G  +   YPL +KR   + LR   + RPRT+T+ +VMR+RNA   A H F
Sbjct: 91  ELKATKLELLGGADPGSYPLQKKRHSFEFLRTIGHLRPRTNTIGAVMRVRNACATAIHQF 150

Query: 240 FQNHSFLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAV 299
           FQ   FL+V  P IT +D EG  + F VTTL                             
Sbjct: 151 FQERGFLWVHTPIITASDCEGAGDLFKVTTL----------------------------- 210

Query: 300 MEKSNLVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFP 359
                   +L+R   N E                                 +++ ++FF 
Sbjct: 211 --------DLQRVPKNGEG--------------------------------IDYSQDFFG 270

Query: 360 QETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAM 419
           ++ YLTVSGQL  E  A A  NVY+ GP FRA+   +++H AE WMVE E+AF +LE   
Sbjct: 271 KQAYLTVSGQLEAEVMALAFQNVYTFGPTFRAENSNTSRHLAEFWMVEPEMAFCDLEGDR 330

Query: 420 NCADDLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKA 479
             A++ +K++ K+VL+ C +DM+F ++RID T +   ++ I+  F  ++Y+ AI+ LEKA
Sbjct: 331 QWAEEFLKYIFKFVLEKCPEDMEFFDQRIDNTVLATADNIINNEFAWLTYSEAIKLLEKA 390

Query: 480 TPDQKHQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAA 539
             DQK ++   + +G  L ++H  YLA+T++K+P IV +YP+  K F +RLNDDGKTVAA
Sbjct: 391 --DQKFEY--PVAWGVDLQSEHERYLAETVFKRPTIVTDYPKDIKAFYMRLNDDGKTVAA 450

Query: 540 FDVVLPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFD 599
            D++ PK G +I GSQ+EERLD+L +RM E G+P E   WYLD+ R+G+V H+GF L F+
Sbjct: 451 MDILAPKIGEIIGGSQREERLDILTQRMQEQGVPEEDLWWYLDLRRYGSVPHAGFGLGFE 456

Query: 600 RFLLFTTGLTDIKDVIPFPRS 617
           R + F TG+ +I+DVIPFPR+
Sbjct: 511 RIVQFMTGMANIRDVIPFPRT 456

[Close]

BLAST of CmoCh17G012660 vs. ExPASy TrEMBL
Match: A0A6J1GR04 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456649 PE=3 SV=1)

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 622/622 (100.00%), Postives = 622/622 (100.00%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP
Sbjct: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60

Query: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120
           LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL
Sbjct: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120

Query: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180
           PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE
Sbjct: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180

Query: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240
           KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS
Sbjct: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300

Query: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360
           LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL
Sbjct: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420
           TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD
Sbjct: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420

Query: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480
           LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK
Sbjct: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480

Query: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540
           HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL
Sbjct: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600
           PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF
Sbjct: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASD 623
           TTGLTDIKDVIPFPRSFGKASD
Sbjct: 601 TTGLTDIKDVIPFPRSFGKASD 622

[Close]

BLAST of CmoCh17G012660 vs. ExPASy TrEMBL
Match: A0A6J1JXS5 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111488466 PE=3 SV=1)

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 603/622 (96.95%), Postives = 611/622 (98.23%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAAQQPTVFTPVTFSKY+KRVLL+TVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP
Sbjct: 1   MAAQQPTVFTPVTFSKYSKRVLLKTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60

Query: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120
           LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGN+LHGGEKLETNVHEPPL
Sbjct: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNHLHGGEKLETNVHEPPL 120

Query: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180
           PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE
Sbjct: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180

Query: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240
           KILHVGKVEKEKYPLSQ RIPMDVLR FSYFRPR +TVASVMRIRNALTFATHTFFQNHS
Sbjct: 181 KILHVGKVEKEKYPLSQTRIPMDVLRNFSYFRPRITTVASVMRIRNALTFATHTFFQNHS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGK++EPTEDKETDGVSLETVKAAVMEKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKKIEPTEDKETDGVSLETVKAAVMEKSN 300

Query: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360
           LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPES KD+VNFHENF PQETYL
Sbjct: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESRKDEVNFHENFLPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420
            VSGQLHLETYACALGNVYSCGPRFR DKIESAKHAAEMWMVEVEIAFAELEDAMNCADD
Sbjct: 361 AVSGQLHLETYACALGNVYSCGPRFRVDKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420

Query: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480
           LVKFLSKWVLDHCVDDMKFVEKRIDKTSI RLESF SCSFEKVSYTAAIESLEKATPDQK
Sbjct: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIQRLESFKSCSFEKVSYTAAIESLEKATPDQK 480

Query: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540
           HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL
Sbjct: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600
           PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYL+IHRHGAV HSGFSLAFDRFLLF
Sbjct: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLEIHRHGAVNHSGFSLAFDRFLLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASD 623
           TTGLTDIKDVIPFPRSFGKA D
Sbjct: 601 TTGLTDIKDVIPFPRSFGKARD 622

[Close]

BLAST of CmoCh17G012660 vs. ExPASy TrEMBL
Match: A0A6J1KTV9 (asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497586 PE=4 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 1.1e-294
Identity = 519/623 (83.31%), Postives = 560/623 (89.89%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAA+ PT FTPVTFSKY+KRVLL+TV +  DGGLGFVD+KLV+GGWVKSSKEV  +A  P
Sbjct: 1   MAAEHPTAFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHE--P 120
             DASV+ AGD LP +AKD SCIEI+QSRIPIVRSLLRMLYGN+LH GEKLET VH+  P
Sbjct: 61  -ADASVRTAGDALPRRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETPVHKPPP 120

Query: 121 PLPSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLE 180
           PLPST FLVVNDGSCVA LQVVVESSLH P  LMP+GTCILVEGILKR P PGKH VRLE
Sbjct: 121 PLPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLE 180

Query: 181 VEKILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQN 240
           VEKILHVGKVE+EKYPL+QK++PMD+LRKFSYFRPRT+TVASVMRI NALTFATHTFFQN
Sbjct: 181 VEKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQN 240

Query: 241 HSFLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEK 300
           H FLYVQLPTITTTDSEGFSEKFHVTT A K   K++EPTEDKETDGVSLETVKAA +EK
Sbjct: 241 HGFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEK 300

Query: 301 SNLVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQET 360
            N+VKELERSE N+EALAAAVQDLK+TNELALQLEARGK K +  KDKV+  ENFFPQET
Sbjct: 301 RNIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSQLKKDKVHISENFFPQET 360

Query: 361 YLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCA 420
           YLTVSGQLHLETYACALGNVYSCGPRFRAD+I SAKHAAEMWMVEVEIAFAELED +NCA
Sbjct: 361 YLTVSGQLHLETYACALGNVYSCGPRFRADRIVSAKHAAEMWMVEVEIAFAELEDVINCA 420

Query: 421 DDLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPD 480
           DDLVKFLSKWVL+HC+DDMKFV+KRIDKTS+ RLES I+ SFEKVSYTAA+E L+KAT D
Sbjct: 421 DDLVKFLSKWVLEHCLDDMKFVQKRIDKTSVHRLESTITSSFEKVSYTAAVEILDKAT-D 480

Query: 481 QKHQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDV 540
           QK Q LK LKFGAALT DHLSYLAD I+KKPVIVYNYP+ TKPFNVRLNDDGKTVAAFDV
Sbjct: 481 QKSQRLKRLKFGAALTDDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDV 540

Query: 541 VLPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFL 600
           VLPKGGVVISGSQKEER DVL+ RM EMGLPREQYEWYL+I RHG VKHSGFSLAFDRF+
Sbjct: 541 VLPKGGVVISGSQKEERFDVLLSRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFV 600

Query: 601 LFTTGLTDIKDVIPFPRSFGKAS 622
           LFTTGLTDIKDVIPFPRS GK S
Sbjct: 601 LFTTGLTDIKDVIPFPRSLGKVS 621

[Close]

BLAST of CmoCh17G012660 vs. ExPASy TrEMBL
Match: A0A5D3BD07 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00300 PE=3 SV=1)

HSP 1 Score: 1020.4 bits (2637), Expect = 3.2e-294
Identity = 525/620 (84.68%), Postives = 563/620 (90.81%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAAQQPTVF+P+TFSKY+ RVLL+T+ ++ DGGL FV +KLVVGGWVKSSKEVI DA  P
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDA--P 60

Query: 61  LVDASVKAAGDVLPLKA-KDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPP 120
              A   A  D LP K  KD SCIEI+QSRIPIVRSLL+MLYGNNLHGGEKL+T VH+PP
Sbjct: 61  PAPAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPP 120

Query: 121 LPSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEV 180
           LPSTAFL +NDGSCVA LQVVVESSLH P HL+P+GTCILVEG+LK++PVPGKH VRLEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNH 240
           EKILHVGKVE+EKYPLSQK++PMD LRKFSYFRPRT+TVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKS 300
           SFLYVQLPTITTTDSEGFSEKFHVTTLA K  GKRVE TEDKE DG+SLETVKAA+ EKS
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGK--GKRVESTEDKEPDGLSLETVKAAITEKS 300

Query: 301 NLVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETY 360
           NLVKELERSE N+EAL AAVQDLK+TN+LALQLEAR KSK +S KDKV    NF PQETY
Sbjct: 301 NLVKELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKV-IPGNFLPQETY 360

Query: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCAD 420
           LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAEL+DAMNCAD
Sbjct: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCAD 420

Query: 421 DLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQ 480
           DLVKFLSKWVL+HC+DDMKFV+KRIDKTSI RLES ISCSFEKVSYTAAIE LEKAT DQ
Sbjct: 421 DLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKAT-DQ 480

Query: 481 KHQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVV 540
           K QHLK LKFGAALTADHLSYLADTI+KKPVIVYNYP+ TKPFNVRLNDDGKTVAAFDVV
Sbjct: 481 KSQHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVV 540

Query: 541 LPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLL 600
           LPKGGVVISGSQKEERLDVLM RM EMGLPREQYEWYL+I RHG VKHSGFSLAFDRF+L
Sbjct: 541 LPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVL 600

Query: 601 FTTGLTDIKDVIPFPRSFGK 620
           FTTGLTDIKDVIPFPRSFGK
Sbjct: 601 FTTGLTDIKDVIPFPRSFGK 614

[Close]

BLAST of CmoCh17G012660 vs. ExPASy TrEMBL
Match: A0A1S3C6Z5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 3.5e-293
Identity = 524/620 (84.52%), Postives = 561/620 (90.48%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAAQQPTVF+P+TFSKY+ RVLL+T+ ++ DGGL FV +KLVVGGWVKSSKEVI DA   
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDA--- 60

Query: 61  LVDASVKAAGDVLPLKA-KDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPP 120
                  A  D LP K  KD SCIEI+QSRIPIVRSLL+MLYGNNLHGGEKL+T VH+PP
Sbjct: 61  ---PPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPP 120

Query: 121 LPSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEV 180
           LPSTAFL +NDGSCVA LQVVVESSLH P HL+P+GTCILVEG+LK++PVPGKH VRLEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNH 240
           EKILHVGKVE+EKYPLSQK++PMD LRKFSYFRPRT+TVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKS 300
           SFLYVQLPTITTTDSEGFSEKFHVTTLA K  GKRVE TEDKE DG+SLETVKAA+ EKS
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGK--GKRVESTEDKEPDGLSLETVKAAITEKS 300

Query: 301 NLVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETY 360
           NLVKELERSE N+EAL AAVQDLK+TNELALQLEAR KSK +S KDKV    NF PQETY
Sbjct: 301 NLVKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFLPQETY 360

Query: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCAD 420
           LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAEL+DAMNCAD
Sbjct: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCAD 420

Query: 421 DLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQ 480
           DLVKFLSKWVL+HC+DDMKFV+KRIDKTSI RLES ISCSFEKVSYTAAIE LEKAT DQ
Sbjct: 421 DLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKAT-DQ 480

Query: 481 KHQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVV 540
           K QHLK LKFGAALTADHLSYLADTI+KKPVIVYNYP+ TKPFNVRLNDDGKTVAAFDVV
Sbjct: 481 KSQHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVV 540

Query: 541 LPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLL 600
           LPKGGVVISGSQKEERLDVLM RM EMGLPREQYEWYL+I RHG VKHSGFSLAFDRF+L
Sbjct: 541 LPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVL 600

Query: 601 FTTGLTDIKDVIPFPRSFGK 620
           FTTGLTDIKDVIPFPRSFGK
Sbjct: 601 FTTGLTDIKDVIPFPRSFGK 610

[Close]

BLAST of CmoCh17G012660 vs. NCBI nr
Match: XP_022954403.1 (asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita moschata])

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 622/622 (100.00%), Postives = 622/622 (100.00%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP
Sbjct: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60

Query: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120
           LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL
Sbjct: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120

Query: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180
           PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE
Sbjct: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180

Query: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240
           KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS
Sbjct: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300

Query: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360
           LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL
Sbjct: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420
           TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD
Sbjct: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420

Query: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480
           LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK
Sbjct: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480

Query: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540
           HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL
Sbjct: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600
           PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF
Sbjct: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASD 623
           TTGLTDIKDVIPFPRSFGKASD
Sbjct: 601 TTGLTDIKDVIPFPRSFGKASD 622

[Close]

BLAST of CmoCh17G012660 vs. NCBI nr
Match: KAG6576013.1 (Asparagine--tRNA ligase, cytoplasmic 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1204.5 bits (3115), Expect = 0.0e+00
Identity = 611/622 (98.23%), Postives = 618/622 (99.36%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAAQQPTVFTPVTFSKY+KRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP
Sbjct: 1   MAAQQPTVFTPVTFSKYSKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60

Query: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120
           LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVH+PPL
Sbjct: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHQPPL 120

Query: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180
           PSTAFLVVNDGSCVA+LQVVVESSLHQPIHLMPSGTCILVEGILKR+ VPGKHTVRLEVE
Sbjct: 121 PSTAFLVVNDGSCVANLQVVVESSLHQPIHLMPSGTCILVEGILKRVSVPGKHTVRLEVE 180

Query: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240
           KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS
Sbjct: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300
           FLYVQLP ITTTDSEGFSEKFHVTTLATKLAGK+VEPTEDKETDGVSLET+KAAVMEKSN
Sbjct: 241 FLYVQLPAITTTDSEGFSEKFHVTTLATKLAGKKVEPTEDKETDGVSLETLKAAVMEKSN 300

Query: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360
           LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL
Sbjct: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420
           TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEM MVEVEIAFAELEDAMNCADD
Sbjct: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMSMVEVEIAFAELEDAMNCADD 420

Query: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480
           LVKFLSKWVLDHCVDDMKFVEKRIDKTSI RLESFISCSFEKVSYTAAIESLEKATPDQK
Sbjct: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIQRLESFISCSFEKVSYTAAIESLEKATPDQK 480

Query: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540
           HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL
Sbjct: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600
           PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYL+IHRHGAVKHSGFSLAFDRFLLF
Sbjct: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLEIHRHGAVKHSGFSLAFDRFLLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASD 623
           TTGLTDIKDVIPFPRSFGKASD
Sbjct: 601 TTGLTDIKDVIPFPRSFGKASD 622

[Close]

BLAST of CmoCh17G012660 vs. NCBI nr
Match: XP_023549170.1 (asparagine--tRNA ligase, cytoplasmic 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 607/622 (97.59%), Postives = 615/622 (98.87%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAAQQPTVFTPVTFS Y+KR+LL+TVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP
Sbjct: 1   MAAQQPTVFTPVTFSNYSKRILLKTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60

Query: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120
           LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGN+LHGGEKLETNV +PPL
Sbjct: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNHLHGGEKLETNVDQPPL 120

Query: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180
           PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE
Sbjct: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180

Query: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240
           KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPR +TVASVMRIRNA TFATHTFFQNHS
Sbjct: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRITTVASVMRIRNASTFATHTFFQNHS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGK+VEPTEDKETDGVSLETVKAAVMEKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKKVEPTEDKETDGVSLETVKAAVMEKSN 300

Query: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360
           LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVN HENFFPQETYL
Sbjct: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNVHENFFPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420
           TVSGQLHLETYACALGNVYSCGPRFRA KIESAKHAAEMWMVEVEIAFAELEDAMNCADD
Sbjct: 361 TVSGQLHLETYACALGNVYSCGPRFRAGKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420

Query: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480
           LVKFLSKWVLDHCVDDMKFVEKRIDKTSI RLESFISCSFEKVSYTAAIESLEKATPDQK
Sbjct: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIQRLESFISCSFEKVSYTAAIESLEKATPDQK 480

Query: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540
           HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL
Sbjct: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600
           PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYL+IHRHGAVKHSGFSLAFDRFLLF
Sbjct: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLEIHRHGAVKHSGFSLAFDRFLLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASD 623
           TTGLTDIKDVIPFPRSFGKASD
Sbjct: 601 TTGLTDIKDVIPFPRSFGKASD 622

[Close]

BLAST of CmoCh17G012660 vs. NCBI nr
Match: XP_022991978.1 (asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita maxima])

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 603/622 (96.95%), Postives = 611/622 (98.23%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAAQQPTVFTPVTFSKY+KRVLL+TVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP
Sbjct: 1   MAAQQPTVFTPVTFSKYSKRVLLKTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60

Query: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120
           LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGN+LHGGEKLETNVHEPPL
Sbjct: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNHLHGGEKLETNVHEPPL 120

Query: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180
           PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE
Sbjct: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180

Query: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240
           KILHVGKVEKEKYPLSQ RIPMDVLR FSYFRPR +TVASVMRIRNALTFATHTFFQNHS
Sbjct: 181 KILHVGKVEKEKYPLSQTRIPMDVLRNFSYFRPRITTVASVMRIRNALTFATHTFFQNHS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGK++EPTEDKETDGVSLETVKAAVMEKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKKIEPTEDKETDGVSLETVKAAVMEKSN 300

Query: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360
           LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPES KD+VNFHENF PQETYL
Sbjct: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESRKDEVNFHENFLPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420
            VSGQLHLETYACALGNVYSCGPRFR DKIESAKHAAEMWMVEVEIAFAELEDAMNCADD
Sbjct: 361 AVSGQLHLETYACALGNVYSCGPRFRVDKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420

Query: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480
           LVKFLSKWVLDHCVDDMKFVEKRIDKTSI RLESF SCSFEKVSYTAAIESLEKATPDQK
Sbjct: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIQRLESFKSCSFEKVSYTAAIESLEKATPDQK 480

Query: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540
           HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL
Sbjct: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600
           PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYL+IHRHGAV HSGFSLAFDRFLLF
Sbjct: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLEIHRHGAVNHSGFSLAFDRFLLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASD 623
           TTGLTDIKDVIPFPRSFGKA D
Sbjct: 601 TTGLTDIKDVIPFPRSFGKARD 622

[Close]

BLAST of CmoCh17G012660 vs. NCBI nr
Match: XP_023549171.1 (asparagine--tRNA ligase, cytoplasmic 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1164.8 bits (3012), Expect = 0.0e+00
Identity = 595/622 (95.66%), Postives = 603/622 (96.95%), Query Frame = 0


Query: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60
           MAAQQPTVFTPVTFS Y+KR+LL+TVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP
Sbjct: 1   MAAQQPTVFTPVTFSNYSKRILLKTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60

Query: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120
           LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGN+LHGGEKLETNV +PPL
Sbjct: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNHLHGGEKLETNVDQPPL 120

Query: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180
           PSTAFL            VVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE
Sbjct: 121 PSTAFL------------VVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180

Query: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240
           KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPR +TVASVMRIRNA TFATHTFFQNHS
Sbjct: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRITTVASVMRIRNASTFATHTFFQNHS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGK+VEPTEDKETDGVSLETVKAAVMEKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKKVEPTEDKETDGVSLETVKAAVMEKSN 300

Query: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360
           LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVN HENFFPQETYL
Sbjct: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNVHENFFPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420
           TVSGQLHLETYACALGNVYSCGPRFRA KIESAKHAAEMWMVEVEIAFAELEDAMNCADD
Sbjct: 361 TVSGQLHLETYACALGNVYSCGPRFRAGKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420

Query: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480
           LVKFLSKWVLDHCVDDMKFVEKRIDKTSI RLESFISCSFEKVSYTAAIESLEKATPDQK
Sbjct: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIQRLESFISCSFEKVSYTAAIESLEKATPDQK 480

Query: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540
           HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL
Sbjct: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600
           PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYL+IHRHGAVKHSGFSLAFDRFLLF
Sbjct: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLEIHRHGAVKHSGFSLAFDRFLLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASD 623
           TTGLTDIKDVIPFPRSFGKASD
Sbjct: 601 TTGLTDIKDVIPFPRSFGKASD 610

[Close]

BLAST of CmoCh17G012660 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 577.0 bits (1486), Expect = 1.8e-164
Identity = 311/625 (49.76%), Postives = 425/625 (68.00%), Query Frame = 0


Query: 11  PVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDA---VRPLVDASVK 70
           P+T SKY+KRV L+T+ +  D G G   +++V+GGWVKS++ V  ++     P+V A   
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  AAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNN----LHGGEKLETNVHEPPLPST 130
           ++G        +  C EI+QS++ I +    +L G      +    +L      PP    
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEYV 139

Query: 131 AFLVVNDGSCVADLQVVVESSLH--QPIHLMPSGTCILVEGILKRIPV--PGKHTVRLEV 190
            + +++DGS ++ LQVVV+S+L       LM  GTCI+ EG+L R+P+    KH + LE 
Sbjct: 140 FYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVL-RLPLAASAKHVIELEA 199

Query: 191 EKILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNH 250
           EK+LHVG V+ EKYPLS+K++P+ +LR FS+FRPRT+TV SV R+ +ALT A+HTF Q H
Sbjct: 200 EKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYH 259

Query: 251 SFLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKS 310
            F YVQ+P ITTT   GF E F VTTL  K   K  +    +E DG S++TVKA + EK+
Sbjct: 260 GFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVIKEKT 319

Query: 311 NLVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPES--SKDKVNFHENFFPQE 370
            L+  L+RS+ N+E + AAV DLK+TN+LA Q+E + KSK  +    +K++F ++FF ++
Sbjct: 320 RLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFGRD 379

Query: 371 TYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNC 430
           TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL+DAM+C
Sbjct: 380 TYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAMDC 439

Query: 431 ADDLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATP 490
           AD+  KFL K+VL++  +DMKF+ KR+DKT   RLE+  S S  + SYT  I  L+KAT 
Sbjct: 440 ADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKATT 499

Query: 491 DQKHQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFD 550
            +        ++G ALT +HLSYL D IYK PVIV+ YP+  K F VRLNDD KTVAAFD
Sbjct: 500 TKFE---TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAFD 559

Query: 551 VVLPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRF 610
           +V+PK GVVI+GSQ EER ++L  R+GE G  RE++EWYLD+ RHG VKHSG SL+ ++ 
Sbjct: 560 LVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQM 619

Query: 611 LLFTTGLTDIKDVIPFPRSFGKASD 623
           LL+ TGL DIKD IPFPRS+GKA++
Sbjct: 620 LLYATGLPDIKDAIPFPRSWGKANN 638

[Close]

BLAST of CmoCh17G012660 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 469.9 bits (1208), Expect = 3.1e-132
Identity = 253/611 (41.41%), Postives = 370/611 (60.56%), Query Frame = 0


Query: 15  SKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRPLVDASVKAAGDVLP 74
           ++++ RVL+RT+ +  DGG G   + + +GGWVKS ++                      
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD---------------------- 86

Query: 75  LKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPLPSTAFLVVNDGSCV 134
                                            G++           + +FL VNDGSC 
Sbjct: 87  --------------------------------QGKR-----------TFSFLAVNDGSCP 146

Query: 135 ADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHT---VRLEVEKILHVGKVEKE 194
           A+LQV+V+ SL+   +L+ +GTC+ V+G+LK +P  GK T   + L V K++ VG V+  
Sbjct: 147 ANLQVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDAS 206

Query: 195 KYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHSFLYVQLPTITT 254
           KYPL + ++ ++ LR   + R RT+++++V RIRNAL FATH+FFQ HSFLY+  P ITT
Sbjct: 207 KYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITT 266

Query: 255 TDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSNLVKELERSEGN 314
           +D EG  E F  TTL         +  ++       +E  +  V+E+ N+V EL+ ++ +
Sbjct: 267 SDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKAS 326

Query: 315 KEALAAAVQDLKRTNELALQLEARGKSKPESSK--DKVNFHENFFPQETYLTVSGQLHLE 374
           KEA+ AAV +LK   E    ++ R + +P   K    +++ ++FF ++ +LTVSGQL +E
Sbjct: 327 KEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVE 386

Query: 375 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADDLVKFLSKWV 434
           TYACAL NVY+ GP FRA+   +++H AE WMVE EIAFA+LED MNCA+  VK++  W+
Sbjct: 387 TYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWL 446

Query: 435 LDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQKHQHLKSLKF 494
           L+ C  DM+ + K  D   I RL+   S  F +++YT AIE LE+A    K +   ++++
Sbjct: 447 LEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGK-EFDNNVEW 506

Query: 495 GAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 554
           G  L ++H  YL + +++KP+IVYNYP+G K F +RLNDD KTVAA DV++PK G +I G
Sbjct: 507 GIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGG 566

Query: 555 SQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLFTTGLTDIKD 614
           SQ+EER DV+ +R+ EMGLP E YEWYLD+ R+G VKH GF L F+R +LF TGL +I+D
Sbjct: 567 SQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRD 570

Query: 615 VIPFPRSFGKA 621
           VIPFPR  GKA
Sbjct: 627 VIPFPRYPGKA 570

[Close]

BLAST of CmoCh17G012660 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 448.4 bits (1152), Expect = 9.7e-126
Identity = 234/503 (46.52%), Postives = 330/503 (65.61%), Query Frame = 0


Query: 124 AFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPG---KHTVRLEVE 183
           AFL VNDGSC A+LQV+V+SSL+    L+ +GTC+ V+G+LK IP  G   K ++ L VE
Sbjct: 68  AFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVE 127

Query: 184 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 243
            ++ VG V+   YPL + ++  + LR   + R RT+ +++V RIRNAL FATH+FFQ HS
Sbjct: 128 TVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHS 187

Query: 244 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 303
           FLY+  P ITT+D EG  E F VTTL         +  ++       +E  +  V E+  
Sbjct: 188 FLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGE 247

Query: 304 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKD--KVNFHENFFPQET 363
            V +L+ ++ +KE + A+V  L         +E R + KP   K+  K+++  +FF ++ 
Sbjct: 248 AVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQA 307

Query: 364 YLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCA 423
           +LTVSGQL +ETYACAL +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA
Sbjct: 308 FLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCA 367

Query: 424 DDLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPD 483
           +  VK++ KW++D C DDM+ ++K +D+    RL      SF++V+YT AIE LEKA   
Sbjct: 368 EAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQ 427

Query: 484 QKHQHLKSLKFGAALTADHLSYLADTIY-KKPVIVYNYPEGTKPFNVRLNDDGKTVAAFD 543
            K      +++G  L ++H  YL +  + +KP+IVYNYP+G K F +RLNDD KTVAA D
Sbjct: 428 GKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMD 487

Query: 544 VVLPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRF 603
           V++PK G +I GSQ+EER DV+ +R+ EMGLP E YEWYLD+ R+G VKH GF L F+R 
Sbjct: 488 VLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERM 547

Query: 604 LLFTTGLTDIKDVIPFPRSFGKA 621
           + F TG+ +I+DVIPFPR  GKA
Sbjct: 548 IQFATGIDNIRDVIPFPRYPGKA 569

[Close]

BLAST of CmoCh17G012660 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 320.1 bits (819), Expect = 4.0e-87
Identity = 189/501 (37.72%), Postives = 291/501 (58.08%), Query Frame = 0


Query: 122 STAFLVVNDGSCVADLQVVVESSLHQPIHL----MPSGTCILVEGILKRIPVPG-KHTVR 181
           S  F+ +NDGSC+++LQ V+ S       +    + +G  + V+G +  +   G K  V 
Sbjct: 126 SVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTI--VASQGTKQKVE 185

Query: 182 LEVEKILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFF 241
           L+VEKI+ VG+ +   YP+ +KR+  + LR  ++ RPRT+T  +V R+RN L +ATH FF
Sbjct: 186 LKVEKIIVVGECD-SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFF 245

Query: 242 QNHSFLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVM 301
           Q   F++V  P IT +D EG  E+F VTTL          P+  + TD  S++ +     
Sbjct: 246 QESGFVWVASPIITASDCEGAGEQFCVTTLI---------PSSHENTD-TSIDAI----- 305

Query: 302 EKSNLVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQ 361
                                                      P++    +++ ++FF +
Sbjct: 306 -------------------------------------------PKTKGGLIDWSQDFFGK 365

Query: 362 ETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMN 421
             +LTVSGQL+ ETYA AL +VY+ GP FRA+   +++H AE WM+E E+AFA+L+D M 
Sbjct: 366 PAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMA 425

Query: 422 CADDLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKAT 481
           CA   ++++ K+VLD+C +DM+F +  I+K  I RL       F ++ YT AIE L KA 
Sbjct: 426 CATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKA- 485

Query: 482 PDQKHQHLKSLKFGAALTADHLSYLADTIY-KKPVIVYNYPEGTKPFNVRLNDDGKTVAA 541
            ++K      +K+G  L ++H  Y+ +  +  +PVI+ +YP+  K F +R NDDGKTVAA
Sbjct: 486 -NKKFDF--PVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAA 545

Query: 542 FDVVLPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFD 601
            D+++P+ G +I GSQ+EERL+VL  R+ E+ L +E Y WYLD+ R+G+V H+GF L F+
Sbjct: 546 MDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFE 561

Query: 602 RFLLFTTGLTDIKDVIPFPRS 617
           R + F TG+ +I+DVIPFPR+
Sbjct: 606 RLVQFVTGIDNIRDVIPFPRT 561

[Close]

BLAST of CmoCh17G012660 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 8.2e-16
Identity = 68/270 (25.19%), Postives = 124/270 (45.93%), Query Frame = 0


Query: 349 FHENFFPQETYLTVSGQLHLETYACA-LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIA 408
           F   +  Q   L  S QLH +   C  L  V+  GP FRA+   + +H  E   ++VE+ 
Sbjct: 292 FRLEYKGQPACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEME 351

Query: 409 FAE-LEDAMNCADDLVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYT 468
             +   + M+  D+L  F+   + + C  +++ V K+     +  L   +  +FE+    
Sbjct: 352 IRKHYSEIMDLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEE---- 411

Query: 469 AAIESLEKATPDQKHQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRL 528
             ++ L++A  +     L  L   +      L  + +    +  I++ YP+  +PF    
Sbjct: 412 -GVQMLKEAGVEV--DPLGDLNTESERKLGQL--VLEKYNTEFYILHRYPKAVRPFYTMT 471

Query: 529 NDDGKTVA-AFDVVLPKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAV 588
             D    + +FDV + +G  +ISG+Q+    +VL +R GE G+  +    Y+D  R+GA 
Sbjct: 472 CADNPLYSNSFDVFI-RGEEIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAP 531

Query: 589 KHSGFSLAFDRFLLFTTGLTDIKDVIPFPR 616
            H GF +  +R ++    L +I+    FPR
Sbjct: 532 LHGGFGVGLERVVMLFCALNNIRKTSLFPR 551

The following BLAST results are available for this feature:

BLAST of CmoCh17G012660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SW95	2.5e-163	49.76	Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 ...	[more]
Q9SW96	4.4e-131	41.41	Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ...	[more]
Q9SSK1	1.4e-124	46.52	Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ...	[more]
Q6MEC9	5.6e-94	39.40	Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G...	[more]
P52276	4.7e-93	39.12	Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117...	[more]

BLAST of CmoCh17G012660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GR04	0.0e+00	100.00	Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456649 PE=3 SV=1	[more]
A0A6J1JXS5	0.0e+00	96.95	Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111488466 PE=3 SV=1	[more]
A0A6J1KTV9	1.1e-294	83.31	asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A5D3BD07	3.2e-294	84.68	Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3C6Z5	3.5e-293	84.52	Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1	[more]

BLAST of CmoCh17G012660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022954403.1	0.0e+00	100.00	asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita moschata]	[more]
KAG6576013.1	0.0e+00	98.23	Asparagine--tRNA ligase, cytoplasmic 2, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023549170.1	0.0e+00	97.59	asparagine--tRNA ligase, cytoplasmic 2-like isoform X1 [Cucurbita pepo subsp. pe...	[more]
XP_022991978.1	0.0e+00	96.95	asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita maxima]	[more]
XP_023549171.1	0.0e+00	95.66	asparagine--tRNA ligase, cytoplasmic 2-like isoform X2 [Cucurbita pepo subsp. pe...	[more]

BLAST of CmoCh17G012660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07420.1	1.8e-164	49.76	asparaginyl-tRNA synthetase 2	[more]
AT5G56680.1	3.1e-132	41.41	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G70980.1	9.7e-126	46.52	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G17300.1	4.0e-87	37.72	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G31180.1	8.2e-16	25.19	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 288..335
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 194..621 e-value: 8.3E-84 score: 283.8
None	No IPR available	PANTHER	PTHR22594:SF36	ASPARAGINE--TRNA LIGASE, CYTOPLASMIC 2-RELATED	coord: 16..621
None	No IPR available	PANTHER	PTHR22594	ASPARTYL/LYSYL-TRNA SYNTHETASE	coord: 16..621
None	No IPR available	CDD	cd04318	EcAsnRS_like_N	coord: 124..187 e-value: 4.56324E-14 score: 65.6645
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 202..616
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 360..372 score: 41.78 coord: 537..553 score: 27.94 coord: 376..389 score: 32.47 coord: 577..591 score: 36.06
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 350..615 e-value: 1.3E-49 score: 169.0
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 202..265 e-value: 1.9E-8 score: 33.8
IPR004522	Asparagine-tRNA ligase	TIGRFAM	TIGR00457	TIGR00457	coord: 123..618 e-value: 1.4E-124 score: 414.3

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh17G012660.1	CmoCh17G012660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006418	tRNA aminoacylation for protein translation
biological_process	GO:0006421	asparaginyl-tRNA aminoacylation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004816	asparagine-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding