CmoCh17G010730 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh17G010730
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Thiamine pyrophosphokinase
Location	Cmo_Chr17: 8923704 .. 8924580 (-)
RNA-Seq Expression	CmoCh17G010730
Synteny	CmoCh17G010730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTCTTTCTGCATCAAGGGTGTAATTTTTCCATTCCTATGTGTCGCTGAAATTCTGGCATTACTTGTTATCATGTATGCATTACTGGCTCAAGATATCCATATTCACATGGTGCAGGGGACCAAGATATTTGATGAATCTGAAGATGAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAGTCTATGCCAAATCTGGAGGAGTCTAATGTAGGTTGGACTTCGTTTGGCTGTTTGCGTTCAATGATTCAATTACTACACTTTGTTCTGTGCTCTGACTTTTCAGATCGGAATTATAATGTTGAGCTGTTCATTTTCAGCTGTGTATACTTGTTGCCGGAGCTCTAGGTGGACGATTCGATCACGAGATAGGAAACATCAATGTACTATGCCGTTTCTCAACTACGCGCATAATCCTTCTGTCTGATGATTGTCTTGTTCATCTTCTGCCAAGGACTCATCACCATGAAATCCATGTTGACTCATCCGTTGAGGGTCCGCATTGTGGTCTCATTCCAATTGGGATGCCTTCTGGAAGCACTACAACCAATGGTCTCCAATGGGATTTGAGTAAGTACTTTCGATATCATGCTTTCAAATTGTTCCCCTCCCGCCTTATCAGTGAATGACTGGCTGTGTCATGTCTTGGGAATTACGAACCTCCACAACGGTATGATATTGTCTACTTTGAATATAAACTTTCATGGCTTTGTTTTTTTTTTTTGTTAACGTTGGATTCATTCCAGCTGACACAAAGACGAAGTTTGGAGGTCTAATTAGCTCATCAAATAAAGTTAAAGGAGAGAAAGTAAGAGTGCTATCTGACACCGATCTTCTCTGGACGATCTCATTAAAAAAGGAATAA

mRNA sequence

ATGGGCTCTTTCTGCATCAAGGGTGTAATTTTTCCATTCCTATGTGTCGCTGAAATTCTGGCATTACTTGTTATCATGTATGCATTACTGGCTCAAGATATCCATATTCACATGGTGCAGGGGACCAAGATATTTGATGAATCTGAAGATGAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAGTCTATGCCAAATCTGGAGGAGTCTAATCTGTGTATACTTGTTGCCGGAGCTCTAGGTGGACGATTCGATCACGAGATAGGAAACATCAATGTACTATGCCGTTTCTCAACTACGCGCATAATCCTTCTGTCTGATGATTGTCTTGTTCATCTTCTGCCAAGGACTCATCACCATGAAATCCATGTTGACTCATCCGTTGAGGGTCCGCATTGTGGTCTCATTCCAATTGGGATGCCTTCTGGAAGCACTACAACCAATGGTCTCCAATGGGATTTGACTGACACAAAGACGAAGTTTGGAGGTCTAATTAGCTCATCAAATAAAGTTAAAGGAGAGAAAGTAAGAGTGCTATCTGACACCGATCTTCTCTGGACGATCTCATTAAAAAAGGAATAA

Coding sequence (CDS)

ATGGGCTCTTTCTGCATCAAGGGTGTAATTTTTCCATTCCTATGTGTCGCTGAAATTCTGGCATTACTTGTTATCATGTATGCATTACTGGCTCAAGATATCCATATTCACATGGTGCAGGGGACCAAGATATTTGATGAATCTGAAGATGAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAGTCTATGCCAAATCTGGAGGAGTCTAATCTGTGTATACTTGTTGCCGGAGCTCTAGGTGGACGATTCGATCACGAGATAGGAAACATCAATGTACTATGCCGTTTCTCAACTACGCGCATAATCCTTCTGTCTGATGATTGTCTTGTTCATCTTCTGCCAAGGACTCATCACCATGAAATCCATGTTGACTCATCCGTTGAGGGTCCGCATTGTGGTCTCATTCCAATTGGGATGCCTTCTGGAAGCACTACAACCAATGGTCTCCAATGGGATTTGACTGACACAAAGACGAAGTTTGGAGGTCTAATTAGCTCATCAAATAAAGTTAAAGGAGAGAAAGTAAGAGTGCTATCTGACACCGATCTTCTCTGGACGATCTCATTAAAAAAGGAATAA

Protein sequence

MGSFCIKGVIFPFLCVAEILALLVIMYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE

Homology

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BLAST of CmoCh17G010730 vs. ExPASy Swiss-Prot
Match: B9DGU7 (Thiamine pyrophosphokinase 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.1e-59
Identity = 109/163 (66.87%), Postives = 131/163 (80.37%), Query Frame = 0


Query: 33  DIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIG 92
           D +I++  GTK+ DES D+DTTDL KC+ YI  S  N E S L IL  GALGGRFDHE G
Sbjct: 97  DFYINL--GTKVIDESHDQDTTDLDKCILYIRHSTLNQETSGLQILATGALGGRFDHEAG 156

Query: 93  NINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNG 152
           N+NVL R+  TRI+LLSDDCL+ LLP+TH HEIH+ SS+EGPHCGLIPIG PS  TTT+G
Sbjct: 157 NLNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSG 216

Query: 153 LQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKK 196
           LQWDL++T+ +FGGLIS+SN VK EK+ V SD+DLLWTIS+KK
Sbjct: 217 LQWDLSNTEMRFGGLISTSNLVKEEKITVESDSDLLWTISIKK 257

[Close]

BLAST of CmoCh17G010730 vs. ExPASy Swiss-Prot
Match: F4IV16 (Thiamine pyrophosphokinase 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.3e-57
Identity = 104/162 (64.20%), Postives = 130/162 (80.25%), Query Frame = 0


Query: 34  IHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIGN 93
           +  ++  GTK+ DES D+DTTDL KC++YI  S  N E S L IL  GALGGRFDHE GN
Sbjct: 96  LDFYVYWGTKVIDESHDQDTTDLDKCISYIRHSTLNQESSRLQILATGALGGRFDHEAGN 155

Query: 94  INVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGL 153
           +NVL R+  TRI+LLSDDCL+ LLP+TH HEIH+ SS++GPHCGLIPIG PS +TTT+GL
Sbjct: 156 LNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGL 215

Query: 154 QWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKK 196
           +WDL++T+ +FGGLIS+SN VK E + V SD+DLLWTIS+KK
Sbjct: 216 KWDLSNTEMRFGGLISTSNLVKEEIITVESDSDLLWTISIKK 257

[Close]

BLAST of CmoCh17G010730 vs. ExPASy Swiss-Prot
Match: Q5JK24 (Thiamine pyrophosphokinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK1 PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 1.7e-57
Identity = 103/154 (66.88%), Postives = 127/154 (82.47%), Query Frame = 0


Query: 41  GTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRF 100
           G +I DES D+DTTDLHKCV++I ++ P  EESNL ILV GALGGRFDHE+GNINVL RF
Sbjct: 110 GAEIVDESHDQDTTDLHKCVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRF 169

Query: 101 STTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDT 160
           S  RI+LLSDDC + LLP+TH HEIH++ S+EGPHCGLIP+G PS STTT GL+W+L +T
Sbjct: 170 SNIRIVLLSDDCSIFLLPKTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNT 229

Query: 161 KTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLK 195
              +GGLIS+SN V+ E VR+ SD+DL+WTISL+
Sbjct: 230 SMSYGGLISTSNIVEEETVRITSDSDLIWTISLR 263

[Close]

BLAST of CmoCh17G010730 vs. ExPASy Swiss-Prot
Match: Q0JMW0 (Thiamine pyrophosphokinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 7.2e-56
Identity = 99/155 (63.87%), Postives = 127/155 (81.94%), Query Frame = 0


Query: 40  QGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCR 99
           QG+KI D+S +++TTDLHKC++ I +  P+ E++NLC+LV GALGGRFDHE  NIN+L  
Sbjct: 110 QGSKISDKSHNQETTDLHKCISRIHRCTPDHEKTNLCVLVTGALGGRFDHEAANINILYL 169

Query: 100 FSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTD 159
           FS  RI+LLSDDCL+ LLP+TH HEI+++SSVEGPHCGL P+G PSGSTTT GL+W+L++
Sbjct: 170 FSDMRIVLLSDDCLIRLLPKTHKHEIYIESSVEGPHCGLFPVGAPSGSTTTTGLKWNLSE 229

Query: 160 TKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLK 195
            K +FG +IS+SN V  EKV V SD DLLWTISL+
Sbjct: 230 AKMRFGSMISTSNIVHAEKVTVQSDADLLWTISLR 264

[Close]

BLAST of CmoCh17G010730 vs. ExPASy Swiss-Prot
Match: Q60DX1 (Thiamine pyrophosphokinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.8e-54
Identity = 102/156 (65.38%), Postives = 122/156 (78.21%), Query Frame = 0


Query: 41  GTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRF 100
           GT+I DES D+DTTDLHKCVA+I ++     +SNLCI   GALGGRFDHE+GNINVL  F
Sbjct: 109 GTQIVDESHDQDTTDLHKCVAFITENSAIPNKSNLCIFALGALGGRFDHEMGNINVLHLF 168

Query: 101 STTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDT 160
              RIILLSDDCL+ LLPRTH H IH++ S+EGPHCGLIPIG PS +TTT GLQW+L +T
Sbjct: 169 PNNRIILLSDDCLIFLLPRTHTHNIHIERSIEGPHCGLIPIGAPSATTTTTGLQWNLDNT 228

Query: 161 KTKFGGLISSSNKVKGEK--VRVLSDTDLLWTISLK 195
              FGGLIS+SN V+ E   V + SD+DL+WTISL+
Sbjct: 229 SMSFGGLISTSNIVREESTVVTITSDSDLIWTISLR 264

[Close]

BLAST of CmoCh17G010730 vs. ExPASy TrEMBL
Match: A0A6J1GPJ0 (Thiamine diphosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=4 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.2e-106
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MGSFCIKGVIFPFLCVAEILALLVIMYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCV 60
           MGSFCIKGVIFPFLCVAEILALLVIMYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCV
Sbjct: 1   MGSFCIKGVIFPFLCVAEILALLVIMYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCV 60

Query: 61  AYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRT 120
           AYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRT
Sbjct: 61  AYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRT 120

Query: 121 HHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVR 180
           HHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVR
Sbjct: 121 HHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVR 180

Query: 181 VLSDTDLLWTISLKKE 197
           VLSDTDLLWTISLKKE
Sbjct: 181 VLSDTDLLWTISLKKE 196

[Close]

BLAST of CmoCh17G010730 vs. ExPASy TrEMBL
Match: A0A6J1GN49 (Thiamine diphosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.4e-85
Identity = 158/171 (92.40%), Postives = 163/171 (95.32%), Query Frame = 0


Query: 26  MYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 85
           M ++  + +  +  QGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG
Sbjct: 1   MDSIRTEVLDFYAKQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 60

Query: 86  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 145
           RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS
Sbjct: 61  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 120

Query: 146 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 197
           GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE
Sbjct: 121 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 171

[Close]

BLAST of CmoCh17G010730 vs. ExPASy TrEMBL
Match: A0A6J1GPJ2 (Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.4e-85
Identity = 158/171 (92.40%), Postives = 163/171 (95.32%), Query Frame = 0


Query: 26  MYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 85
           M ++  + +  +  QGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG
Sbjct: 90  MDSIRTEVLDFYAKQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 149

Query: 86  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 145
           RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS
Sbjct: 150 RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 209

Query: 146 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 197
           GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE
Sbjct: 210 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 260

[Close]

BLAST of CmoCh17G010730 vs. ExPASy TrEMBL
Match: A0A6J1JVX6 (Thiamine diphosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111488361 PE=4 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 8.0e-82
Identity = 153/171 (89.47%), Postives = 160/171 (93.57%), Query Frame = 0


Query: 26  MYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 85
           M ++ A+ +  +  QG KIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG
Sbjct: 1   MDSIRAEVLDFYDKQGIKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 60

Query: 86  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 145
           RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS
Sbjct: 61  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 120

Query: 146 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 197
           GSTTT GLQWDLTDT+ KFGGLISSSNKVKGEKVRV SDTDLLWTISLKK+
Sbjct: 121 GSTTTKGLQWDLTDTEMKFGGLISSSNKVKGEKVRVQSDTDLLWTISLKKQ 171

[Close]

BLAST of CmoCh17G010730 vs. ExPASy TrEMBL
Match: A0A6J1JRW1 (Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111488361 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 8.0e-82
Identity = 153/171 (89.47%), Postives = 160/171 (93.57%), Query Frame = 0


Query: 26  MYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 85
           M ++ A+ +  +  QG KIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG
Sbjct: 93  MDSIRAEVLDFYDKQGIKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 152

Query: 86  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 145
           RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS
Sbjct: 153 RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 212

Query: 146 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 197
           GSTTT GLQWDLTDT+ KFGGLISSSNKVKGEKVRV SDTDLLWTISLKK+
Sbjct: 213 GSTTTKGLQWDLTDTEMKFGGLISSSNKVKGEKVRVQSDTDLLWTISLKKQ 263

[Close]

BLAST of CmoCh17G010730 vs. NCBI nr
Match: XP_022953400.1 (thiamine pyrophosphokinase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 395.6 bits (1015), Expect = 2.5e-106
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MGSFCIKGVIFPFLCVAEILALLVIMYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCV 60
           MGSFCIKGVIFPFLCVAEILALLVIMYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCV
Sbjct: 1   MGSFCIKGVIFPFLCVAEILALLVIMYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCV 60

Query: 61  AYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRT 120
           AYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRT
Sbjct: 61  AYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRT 120

Query: 121 HHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVR 180
           HHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVR
Sbjct: 121 HHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVR 180

Query: 181 VLSDTDLLWTISLKKE 197
           VLSDTDLLWTISLKKE
Sbjct: 181 VLSDTDLLWTISLKKE 196

[Close]

BLAST of CmoCh17G010730 vs. NCBI nr
Match: XP_022953401.1 (thiamine pyrophosphokinase 1-like isoform X3 [Cucurbita moschata] >XP_022953402.1 thiamine pyrophosphokinase 1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 324.3 bits (830), Expect = 7.1e-85
Identity = 158/171 (92.40%), Postives = 163/171 (95.32%), Query Frame = 0


Query: 26  MYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 85
           M ++  + +  +  QGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG
Sbjct: 1   MDSIRTEVLDFYAKQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 60

Query: 86  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 145
           RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS
Sbjct: 61  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 120

Query: 146 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 197
           GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE
Sbjct: 121 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 171

[Close]

BLAST of CmoCh17G010730 vs. NCBI nr
Match: XP_022953399.1 (thiamine pyrophosphokinase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 324.3 bits (830), Expect = 7.1e-85
Identity = 158/171 (92.40%), Postives = 163/171 (95.32%), Query Frame = 0


Query: 26  MYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 85
           M ++  + +  +  QGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG
Sbjct: 90  MDSIRTEVLDFYAKQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 149

Query: 86  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 145
           RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS
Sbjct: 150 RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 209

Query: 146 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 197
           GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE
Sbjct: 210 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 260

[Close]

BLAST of CmoCh17G010730 vs. NCBI nr
Match: XP_023547602.1 (thiamine pyrophosphokinase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 314.7 bits (805), Expect = 5.6e-82
Identity = 153/171 (89.47%), Postives = 160/171 (93.57%), Query Frame = 0


Query: 26  MYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 85
           M ++  + +  +  QGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG
Sbjct: 89  MDSIRTEVLDFYAKQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 148

Query: 86  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 145
           RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS
Sbjct: 149 RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 208

Query: 146 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 197
           GSTTTNGLQWDLTD + KFGGLISSSNKVKGEKVRV SDTDLLWTISLKK+
Sbjct: 209 GSTTTNGLQWDLTDIEMKFGGLISSSNKVKGEKVRVQSDTDLLWTISLKKQ 259

[Close]

BLAST of CmoCh17G010730 vs. NCBI nr
Match: XP_023547604.1 (thiamine pyrophosphokinase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 314.7 bits (805), Expect = 5.6e-82
Identity = 153/171 (89.47%), Postives = 160/171 (93.57%), Query Frame = 0


Query: 26  MYALLAQDIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 85
           M ++  + +  +  QGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG
Sbjct: 1   MDSIRTEVLDFYAKQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGG 60

Query: 86  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 145
           RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS
Sbjct: 61  RFDHEIGNINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPS 120

Query: 146 GSTTTNGLQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKKE 197
           GSTTTNGLQWDLTD + KFGGLISSSNKVKGEKVRV SDTDLLWTISLKK+
Sbjct: 121 GSTTTNGLQWDLTDIEMKFGGLISSSNKVKGEKVRVQSDTDLLWTISLKKQ 171

[Close]

BLAST of CmoCh17G010730 vs. TAIR 10
Match: AT1G02880.1 (thiamin pyrophosphokinase1 )

HSP 1 Score: 229.2 bits (583), Expect = 2.9e-60
Identity = 109/163 (66.87%), Postives = 131/163 (80.37%), Query Frame = 0


Query: 33  DIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIG 92
           D +I++  GTK+ DES D+DTTDL KC+ YI  S  N E S L IL  GALGGRFDHE G
Sbjct: 27  DFYINL--GTKVIDESHDQDTTDLDKCILYIRHSTLNQETSGLQILATGALGGRFDHEAG 86

Query: 93  NINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNG 152
           N+NVL R+  TRI+LLSDDCL+ LLP+TH HEIH+ SS+EGPHCGLIPIG PS  TTT+G
Sbjct: 87  NLNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSG 146

Query: 153 LQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKK 196
           LQWDL++T+ +FGGLIS+SN VK EK+ V SD+DLLWTIS+KK
Sbjct: 147 LQWDLSNTEMRFGGLISTSNLVKEEKITVESDSDLLWTISIKK 187

[Close]

BLAST of CmoCh17G010730 vs. TAIR 10
Match: AT1G02880.2 (thiamin pyrophosphokinase1 )

HSP 1 Score: 229.2 bits (583), Expect = 2.9e-60
Identity = 109/163 (66.87%), Postives = 131/163 (80.37%), Query Frame = 0


Query: 33  DIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIG 92
           D +I++  GTK+ DES D+DTTDL KC+ YI  S  N E S L IL  GALGGRFDHE G
Sbjct: 94  DFYINL--GTKVIDESHDQDTTDLDKCILYIRHSTLNQETSGLQILATGALGGRFDHEAG 153

Query: 93  NINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNG 152
           N+NVL R+  TRI+LLSDDCL+ LLP+TH HEIH+ SS+EGPHCGLIPIG PS  TTT+G
Sbjct: 154 NLNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSG 213

Query: 153 LQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKK 196
           LQWDL++T+ +FGGLIS+SN VK EK+ V SD+DLLWTIS+KK
Sbjct: 214 LQWDLSNTEMRFGGLISTSNLVKEEKITVESDSDLLWTISIKK 254

[Close]

BLAST of CmoCh17G010730 vs. TAIR 10
Match: AT1G02880.3 (thiamin pyrophosphokinase1 )

HSP 1 Score: 229.2 bits (583), Expect = 2.9e-60
Identity = 109/163 (66.87%), Postives = 131/163 (80.37%), Query Frame = 0


Query: 33  DIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIG 92
           D +I++  GTK+ DES D+DTTDL KC+ YI  S  N E S L IL  GALGGRFDHE G
Sbjct: 97  DFYINL--GTKVIDESHDQDTTDLDKCILYIRHSTLNQETSGLQILATGALGGRFDHEAG 156

Query: 93  NINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNG 152
           N+NVL R+  TRI+LLSDDCL+ LLP+TH HEIH+ SS+EGPHCGLIPIG PS  TTT+G
Sbjct: 157 NLNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSG 216

Query: 153 LQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKK 196
           LQWDL++T+ +FGGLIS+SN VK EK+ V SD+DLLWTIS+KK
Sbjct: 217 LQWDLSNTEMRFGGLISTSNLVKEEKITVESDSDLLWTISIKK 257

[Close]

BLAST of CmoCh17G010730 vs. TAIR 10
Match: AT1G02880.4 (thiamin pyrophosphokinase1 )

HSP 1 Score: 229.2 bits (583), Expect = 2.9e-60
Identity = 109/163 (66.87%), Postives = 131/163 (80.37%), Query Frame = 0


Query: 33  DIHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIG 92
           D +I++  GTK+ DES D+DTTDL KC+ YI  S  N E S L IL  GALGGRFDHE G
Sbjct: 10  DFYINL--GTKVIDESHDQDTTDLDKCILYIRHSTLNQETSGLQILATGALGGRFDHEAG 69

Query: 93  NINVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNG 152
           N+NVL R+  TRI+LLSDDCL+ LLP+TH HEIH+ SS+EGPHCGLIPIG PS  TTT+G
Sbjct: 70  NLNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSG 129

Query: 153 LQWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKK 196
           LQWDL++T+ +FGGLIS+SN VK EK+ V SD+DLLWTIS+KK
Sbjct: 130 LQWDLSNTEMRFGGLISTSNLVKEEKITVESDSDLLWTISIKK 170

[Close]

BLAST of CmoCh17G010730 vs. TAIR 10
Match: AT2G44750.2 (thiamin pyrophosphokinase 2 )

HSP 1 Score: 224.2 bits (570), Expect = 9.3e-59
Identity = 104/162 (64.20%), Postives = 130/162 (80.25%), Query Frame = 0


Query: 34  IHIHMVQGTKIFDESEDEDTTDLHKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIGN 93
           +  ++  GTK+ DES D+DTTDL KC++YI  S  N E S L IL  GALGGRFDHE GN
Sbjct: 96  LDFYVYWGTKVIDESHDQDTTDLDKCISYIRHSTLNQESSRLQILATGALGGRFDHEAGN 155

Query: 94  INVLCRFSTTRIILLSDDCLVHLLPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGL 153
           +NVL R+  TRI+LLSDDCL+ LLP+TH HEIH+ SS++GPHCGLIPIG PS +TTT+GL
Sbjct: 156 LNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGL 215

Query: 154 QWDLTDTKTKFGGLISSSNKVKGEKVRVLSDTDLLWTISLKK 196
           +WDL++T+ +FGGLIS+SN VK E + V SD+DLLWTIS+KK
Sbjct: 216 KWDLSNTEMRFGGLISTSNLVKEEIITVESDSDLLWTISIKK 257

The following BLAST results are available for this feature:

BLAST of CmoCh17G010730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9DGU7	4.1e-59	66.87	Thiamine pyrophosphokinase 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=2 SV=1	[more]
F4IV16	1.3e-57	64.20	Thiamine pyrophosphokinase 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1	[more]
Q5JK24	1.7e-57	66.88	Thiamine pyrophosphokinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK1 PE...	[more]
Q0JMW0	7.2e-56	63.87	Thiamine pyrophosphokinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK2 PE...	[more]
Q60DX1	1.8e-54	65.38	Thiamine pyrophosphokinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK3 PE...	[more]

BLAST of CmoCh17G010730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GPJ0	1.2e-106	100.00	Thiamine diphosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=4 SV=1	[more]
A0A6J1GN49	3.4e-85	92.40	Thiamine diphosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=4 SV=1	[more]
A0A6J1GPJ2	3.4e-85	92.40	Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=3 SV...	[more]
A0A6J1JVX6	8.0e-82	89.47	Thiamine diphosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111488361 PE=4 SV=1	[more]
A0A6J1JRW1	8.0e-82	89.47	Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111488361 PE=3 SV=1	[more]

BLAST of CmoCh17G010730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022953400.1	2.5e-106	100.00	thiamine pyrophosphokinase 1-like isoform X2 [Cucurbita moschata]	[more]
XP_022953401.1	7.1e-85	92.40	thiamine pyrophosphokinase 1-like isoform X3 [Cucurbita moschata] >XP_022953402....	[more]
XP_022953399.1	7.1e-85	92.40	thiamine pyrophosphokinase 1-like isoform X1 [Cucurbita moschata]	[more]
XP_023547602.1	5.6e-82	89.47	thiamine pyrophosphokinase 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023547604.1	5.6e-82	89.47	thiamine pyrophosphokinase 1-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmoCh17G010730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02880.1	2.9e-60	66.87	thiamin pyrophosphokinase1	[more]
AT1G02880.2	2.9e-60	66.87	thiamin pyrophosphokinase1	[more]
AT1G02880.3	2.9e-60	66.87	thiamin pyrophosphokinase1	[more]
AT1G02880.4	2.9e-60	66.87	thiamin pyrophosphokinase1	[more]
AT2G44750.2	9.3e-59	64.20	thiamin pyrophosphokinase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007373	Thiamin pyrophosphokinase, thiamin-binding domain	SMART	SM00983	TPK_B1_binding_a_2_a	coord: 121..187 e-value: 3.4E-9 score: 46.5
IPR007373	Thiamin pyrophosphokinase, thiamin-binding domain	PFAM	PF04265	TPK_B1_binding	coord: 122..188 e-value: 5.3E-20 score: 70.9
IPR036759	Thiamin pyrophosphokinase, catalytic domain superfamily	GENE3D	3.40.50.10240	Thiamin pyrophosphokinase, catalytic domain	coord: 30..195 e-value: 9.5E-46 score: 157.8
IPR036759	Thiamin pyrophosphokinase, catalytic domain superfamily	SUPERFAMILY	63999	Thiamin pyrophosphokinase, catalytic domain	coord: 39..113
IPR006282	Thiamin pyrophosphokinase	TIGRFAM	TIGR01378	TIGR01378	coord: 40..191 e-value: 1.5E-35 score: 120.5
IPR007371	Thiamin pyrophosphokinase, catalytic domain	PFAM	PF04263	TPK_catalytic	coord: 39..105 e-value: 5.2E-17 score: 62.0
IPR007371	Thiamin pyrophosphokinase, catalytic domain	CDD	cd07995	TPK	coord: 40..191 e-value: 1.88121E-30 score: 108.786
None	No IPR available	PANTHER	PTHR13622	THIAMIN PYROPHOSPHOKINASE	coord: 40..194
None	No IPR available	PANTHER	PTHR13622:SF12	THIAMINE PYROPHOSPHOKINASE 1	coord: 40..194
IPR036371	Thiamin pyrophosphokinase, thiamin-binding domain superfamily	SUPERFAMILY	63862	Thiamin pyrophosphokinase, substrate-binding domain	coord: 116..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh17G010730.1	CmoCh17G010730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009229	thiamine diphosphate biosynthetic process
biological_process	GO:0006772	thiamine metabolic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0030975	thiamine binding
molecular_function	GO:0004788	thiamine diphosphokinase activity