CmoCh17G005740 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh17G005740
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionBiotin synthase
LocationCmo_Chr17: 5898920 .. 5904200 (+)
RNA-Seq ExpressionCmoCh17G005740
SyntenyCmoCh17G005740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAATCAAGGGTGGGCGGAGATCACAGTGCTGTTGTTGCTGGCTTTGGCCGTTGAGCTACTACCCACTCACTCTCCTTTGCTCTTCTCTGTCATCACTCCGTCTCGCCGGAAAATTCTTCCGCCTTCCGTCCATAAACAGCCACCGAAATGCTATTTGCTCGATCGTTTCTCCGCTCCCACCTCAAACCCTCCGACATTCCTCGCCATTCTCCGCCTTTTTCTTCCCTCTTTTCGTCTCTTTCAGCTTCTTCCGCTGCGTCTATTGAAGCCGATAGGCTTCTCAGAGATGGCCCCAGGAATAATTGGACTCGACAGGAGATTCGCGACATTTACGACTCTCCCGTTCTCGATCTGCTTTTCCATGGCGTCAGTACTCTCTCTCTCTCTCTCTCTATATATATGTGTGTGTGTGTGTGTAATGCAAACTTTTTGTTAGTACTGGTGGGAGAAATTGAAGCTATTTGTTTGAGATTTTTCGTTATTTTCTTATGACTTGTTGGAGACATTGAGTTGGCCTGTAGTCTCAGTGTCTAAGAGCTTTGCACGTACGATTTGTGGTTGATAGGTCTTTTAATGGTTGAAGTTTTTCAATCTGTTCTCAAATCTATTATCACTATCGCTAGAATTAAAAAGTGAAACCCTTACCACGATTTCTTACCTCAAATTTATCGTGTCTGTTAAAGAGGAAATCATCCACAACCTTTTTGCTTATAGATGTTGATTCTAATGGGGTTCTCCTTTGAGGCTTCAGTAGTATTGCCCAACATATAGAATTAGGCCCGTGATGCGAATGTAAATTCATTTAAATGTTCGTAAAAAGTAACTTCCCTTTCTTCTCTTGAACTATCATAGTTGCATAAAAAATAACCACTCCTAGTTACTATATCTCTTGAGAGAAATAAAACTTTTGGAGCATAACTCTGAAGCCACCGCACCAATGTTCTAGGCTGGTGCCTCTCTGAAAGTAACTTGTGTTCATTTCATAAATTGGTCTAATGGTCCAAGGGCGTTATACTATACGAAATGGTGGTTGTTGCATTGGTCTTGTTCGTTTTTGTGGCTAAATGCCTATTCATCCCTCTAAAGGCACAATGGATCTTCTAATATATCATGAAAATAAATAATGATCTATATATCTAACCTAATTCCAATTGTATACCTTGTTTTAGTAACCAATTATTTGCGTATGGAAACTTTTTACTAACAAATAATTTGGTGGAAACTGACTTATTACACAAGTAGTGTTACAATTTTTTTGATTTCCATATTGCAACTTGACCTCTTTCTAAATCATCTAGTTCTTAAATGGGTTTTACTTGAAGCACTATTTCCATACTTTTTCCCCTTTTTATTTTGTTTGCTTATTACATATATATACCTATTTATTGTTCTTCAGGCGCAAGTTCATAGGCACACACACAATTTTCGGGAGGTGCAGCAGTGTACCCTCCTTTCAATCAAAACAGGTGGGTGTAGTGAGGATTGTTCCTACTGTCCTCAATCATCGAGATATGACACTGGAGTGAAATCCCAAAAACTTATGACCAAGGATGTCGTGCTTGAGGCAGCCAAAAAGGTATCCCTTTTTAATTGTTGCAAATTGTTCTGTACATTGAAGCTGAGTTTCATTGCCGCGACTAACCAATAATAACCTTATGTATGTCTTAGATAAGTTGGAATTGGTACCCTTTGGCAGTCTTGTTATGCCATCTTCCTGATCCTTGGGTTTTTGTTGCCCCCTTACTGATCAAGAGACTTGTGAGGTGTCAGTCTCCTTTCTGTTGTTTTTACCTGGTCGTTTGCCATGGAAGAAAGGATTCTAGCTTTTAGCTTTCGAAGCCCTTTGAGGGTTTCTCTAGCAGATATATATATATATATATATTCCGTTGCAGATTCTTTTTGCCCCTTCTCCCCACCAGGAAGCCAATGTATTTTCCTCTGTTTTGGAAGTTAAAATTCCTAAGAAAGTGATGTTCTTTTCTTTGACAAGTTTTACATGGGAGAGTAAATACTCAGAACCGTCTCCAGAGGTTCTCTTCTCTTCCTTGGTGTTGTAGCCTCAATGACGTGTTCTTTGTAGAAGGCAAGAAGAGGATCTTGACCATTTGCTTTGGGATTTTTATTCTGTTAGTTCTTTGTGGAATCCGTTCCTTATGACTTTCAAGATTCTGTTAGCTCATAATAGAGAATATCCATAAATTGTATGTTTGGTATTTATGGTTACTCGGCATTAAATATATTTTATTGAACAAGGCTTTTATTCTAAAAATATTTAGGGCATCTAATGTGCACTGCACATGAACCTTCAATCTTCGTATGCTAAATCATGACATGCTTATTGGTAGACTTTTTGTAACTTAGTACTGGTGTCTTTCATATTTTTCATTCTACCATTGATGTCGTTTTATATATATATAACAGAGCTATACCTTAACTGTTCCTCTTTTGTTTTCATGCCATGCCATCTTCTTTCTTTAAAAAGAAGTTGAAAAATCTTAAAAAGGAATAATTTTTTATTGCATATTATCTGTCCCTTGACCAATATTTAAAGCGTTTATTCACATTGTCTGGCAGGCAAAAGAGGCTGGTAGCACAAGATTTTGTATGGGTGCTGCATGGAGAGATACAATAGGAAGAAAAACTAATTTCAATCAGATCCTTGATTATGTAAAAAAAATAAGGTAACAAATTTCACCTTAAATAAGAAATAGCTACAAAGATTAGAAGACTGAAATAATTAGCTTGCTTACAGAATACAGATTAAAGATAGATCAACAATAAAATGAGAATAAGTTCTTAACTTTTCCTGCCCCTGTCTGCTTAGAATGGTCAGGACAAGAAAGTTTTTCATCATAGAGTGAGTGATGGTCAGAGCAGAGCACTTAGAAAGATTTTGCAATGATTTAAAAAACAAATTCAGCATGATTTGAAAGTCCCTAGTGTTACACATGATTTTTGCCTCTTTTATTTTCTTTAAATACTAGGAAAGAAAATGCTAGGATTCCTTTACTAAATTCAATCTCTTAAGCAAAGCCTTTGTCTTTTCTCCTTTTGCTTAATCTCTCTTTCAAGGCTTGCAGTTCGGTTTTCCGCATCTTTTGATAATTTATGTATATATGTAAAAGATTTCTGCAAGTGTTGAGATTAGCAAGGGTTGACTCTTTAGTATTGTCCAATCAAGCTAGTGAAATGCATGATCGAGAAACTGAAACATTGGCAAGAATCGTCCACAATTATATCTCTTTTAACCTGAGTAATCACTCGTCCTTCGACATTAATTCTCAAATCAAATTCATCTGTGATGTATTAGAGAGATGGGGATGGAGGTCTGCTGCACTCTGGGCATGATAGAGAAGCAACAAGCTATTGACCTCAAGAAGGCCGGCCTCACAGCTTATAACCACAACCTCGACACATCAAGGGAATACTACCCAAATATTATTACCACGAGGAGTTATGATGAGCGTTTAAAAACTCTAGAATTTGTTCGTGATGCTGGAATCAATGTCTGCTCTGGTAATTTTTACATCTTTGATTTTAACTCAGATTTTCCGTGTTGTCCGTCTTCTCTTCAACATTTATACTACTACTTCATGACGTATTGACAAATTTAATGAATATTCCTAGATTTATAACTTATTTATCGGAAAAAGTTTATTAATTTATAACTTCTAGAAGAAATGGCTTATAAAAGAGCGGGAAACCCCCACAAACCATAAGACTACAAAAGGGCTGCCCAGTTGGCATTTGGAAACTATTTAGGATTCATAACACATATAAGGGAAAATATTCAGCTTGGTCAGTAAGCCTAGAAGTCTACTAAAAGTTATAATTGTGACAATTTGAAGAGGATAATATTTATTGAAGGGAGACATAGCTATAGCCTTACGATGGTTTAAGGTTTCATCCATTAGTAGACCAACTATCCCAATGGTTATAAATGGTGGAGGGATCCGAGGACGTTAAGTTTCTGAAGATCTCCACGGCTTCTAGGTTCTTGATTTGGAGTATTCACTTCCCTGATGATGTAGGGAAAGGAATGTTAATGAAGGGAGACGTGTATTCCTTTGTTTGCTTTTGAAACAATTAATTTATATTGATCAGTATTGGCGTTCTATGATTTTTTTTCTAGTTCTCTAATGGGGCCATATAAACATTCCAGAGACTTTTTCCTTTTTCCTTGGCCGCTATATGGTGGTGTACACTTTCTTTTGTATGATAGAAAAATATTCTATTGATGTGATGAAATATACCAAGAAAATTTCCGGGAGCCTGAGAAATTTTGCAACTCCATCCTCTTCCTCCCCTTTTGTACTATATTTTTATGGCATTGGTACTTTCACTGCTTAATAAGAACTTGGTGAAATCCTTAATTTCTGGCAATAGGAGGAATAATTGGGCTTGGAGAGGCAGAAGAGGACCGTGTCGGTTTGCTGCATACCTTGGCGACTCTGCCCACACACCCCGAGAGTGTTCCCATCAATGCGCTGGTTGCAGTGAAAGGAACCCCTCTTCAAGACCAAAAAGTAGCTCATATTTGTTGCACAAACCTTATTTAGACACCACTACTTCACTTCATATGAAAGCTATTGCTTTGGTCTCTATAGTGAATGCTTTTCTTGCCTTTCTTTCTTTTCAGCCTGTTGAAATATGGGAGATGATTCGAATGATCGCCACAGCACGGATCACCATGCCAAAAGCAATGGTAAGGCTGTCTGCAGGTCGAGTGAAGTTTTCCGTGCCGGAGCAGGCGTTATGCTTCCTTGCAGGAGCAAATTCTATCTTCACCGGGGAGAAACTGCTAACGACGCCAAATAACGACTTTGATGCTGATCAACACATGTTCAAGATGCTGGGTTTGATTCCAAAAGCTCCCAGTTTCACTGATGGTGCTGAGAAAGCCCCTGAAGAAGAGTCATTACAAGAAGCTGCTTCCAATTAATTCATAAGCCACTGGCTCTCCAATCAATTCTCTCTCTCTCAATCTTTATTTCTTTGCTTTTTTCTCCATTTTGGGTTTGTTAATCCGTAGATTTGCAGTTGGTATCTCCATTAGATGTGAGCTTAAAAGAGAAAATGAAGAGGTAATGAACACTTCTCCTTGTGTTAGAGTAGTTACACGTACTTGTTCTACAAGTCATCACTTCATGATTAATGTTTAGGAAATCTTTATCATTCCCATTCCTTTTGTTTGTGATAGGACTGGTTATTAATGTATAATGGTTATAGTTTTATAAAATTATTTGACTATTCTCCCTCTTGGCATAAA

mRNA sequence

AAAAAAAAAAAAAAATCAAGGGTGGGCGGAGATCACAGTGCTGTTGTTGCTGGCTTTGGCCGTTGAGCTACTACCCACTCACTCTCCTTTGCTCTTCTCTGTCATCACTCCGTCTCGCCGGAAAATTCTTCCGCCTTCCGTCCATAAACAGCCACCGAAATGCTATTTGCTCGATCGTTTCTCCGCTCCCACCTCAAACCCTCCGACATTCCTCGCCATTCTCCGCCTTTTTCTTCCCTCTTTTCGTCTCTTTCAGCTTCTTCCGCTGCGTCTATTGAAGCCGATAGGCTTCTCAGAGATGGCCCCAGGAATAATTGGACTCGACAGGAGATTCGCGACATTTACGACTCTCCCGTTCTCGATCTGCTTTTCCATGGCGCGCAAGTTCATAGGCACACACACAATTTTCGGGAGGTGCAGCAGTGTACCCTCCTTTCAATCAAAACAGGTGGGTGTAGTGAGGATTGTTCCTACTGTCCTCAATCATCGAGATATGACACTGGAGTGAAATCCCAAAAACTTATGACCAAGGATGTCGTGCTTGAGGCAGCCAAAAAGGCAAAAGAGGCTGGTAGCACAAGATTTTGTATGGGTGCTGCATGGAGAGATACAATAGGAAGAAAAACTAATTTCAATCAGATCCTTGATTATGTAAAAAAAATAAGAGAGATGGGGATGGAGGTCTGCTGCACTCTGGGCATGATAGAGAAGCAACAAGCTATTGACCTCAAGAAGGCCGGCCTCACAGCTTATAACCACAACCTCGACACATCAAGGGAATACTACCCAAATATTATTACCACGAGGAGTTATGATGAGCGTTTAAAAACTCTAGAATTTGTTCGTGATGCTGGAATCAATGTCTGCTCTGGAGGAATAATTGGGCTTGGAGAGGCAGAAGAGGACCGTGTCGGTTTGCTGCATACCTTGGCGACTCTGCCCACACACCCCGAGAGTGTTCCCATCAATGCGCTGGTTGCAGTGAAAGGAACCCCTCTTCAAGACCAAAAACCTGTTGAAATATGGGAGATGATTCGAATGATCGCCACAGCACGGATCACCATGCCAAAAGCAATGGTAAGGCTGTCTGCAGGTCGAGTGAAGTTTTCCGTGCCGGAGCAGGCGTTATGCTTCCTTGCAGGAGCAAATTCTATCTTCACCGGGGAGAAACTGCTAACGACGCCAAATAACGACTTTGATGCTGATCAACACATGTTCAAGATGCTGGGTTTGATTCCAAAAGCTCCCAGTTTCACTGATGGTGCTGAGAAAGCCCCTGAAGAAGAGTCATTACAAGAAGCTGCTTCCAATTAATTCATAAGCCACTGGCTCTCCAATCAATTCTCTCTCTCTCAATCTTTATTTCTTTGCTTTTTTCTCCATTTTGGGTTTGTTAATCCGTAGATTTGCAGTTGGTATCTCCATTAGATGTGAGCTTAAAAGAGAAAATGAAGAGGTAATGAACACTTCTCCTTGTGTTAGAGTAGTTACACGTACTTGTTCTACAAGTCATCACTTCATGATTAATGTTTAGGAAATCTTTATCATTCCCATTCCTTTTGTTTGTGATAGGACTGGTTATTAATGTATAATGGTTATAGTTTTATAAAATTATTTGACTATTCTCCCTCTTGGCATAAA

Coding sequence (CDS)

ATGCTATTTGCTCGATCGTTTCTCCGCTCCCACCTCAAACCCTCCGACATTCCTCGCCATTCTCCGCCTTTTTCTTCCCTCTTTTCGTCTCTTTCAGCTTCTTCCGCTGCGTCTATTGAAGCCGATAGGCTTCTCAGAGATGGCCCCAGGAATAATTGGACTCGACAGGAGATTCGCGACATTTACGACTCTCCCGTTCTCGATCTGCTTTTCCATGGCGCGCAAGTTCATAGGCACACACACAATTTTCGGGAGGTGCAGCAGTGTACCCTCCTTTCAATCAAAACAGGTGGGTGTAGTGAGGATTGTTCCTACTGTCCTCAATCATCGAGATATGACACTGGAGTGAAATCCCAAAAACTTATGACCAAGGATGTCGTGCTTGAGGCAGCCAAAAAGGCAAAAGAGGCTGGTAGCACAAGATTTTGTATGGGTGCTGCATGGAGAGATACAATAGGAAGAAAAACTAATTTCAATCAGATCCTTGATTATGTAAAAAAAATAAGAGAGATGGGGATGGAGGTCTGCTGCACTCTGGGCATGATAGAGAAGCAACAAGCTATTGACCTCAAGAAGGCCGGCCTCACAGCTTATAACCACAACCTCGACACATCAAGGGAATACTACCCAAATATTATTACCACGAGGAGTTATGATGAGCGTTTAAAAACTCTAGAATTTGTTCGTGATGCTGGAATCAATGTCTGCTCTGGAGGAATAATTGGGCTTGGAGAGGCAGAAGAGGACCGTGTCGGTTTGCTGCATACCTTGGCGACTCTGCCCACACACCCCGAGAGTGTTCCCATCAATGCGCTGGTTGCAGTGAAAGGAACCCCTCTTCAAGACCAAAAACCTGTTGAAATATGGGAGATGATTCGAATGATCGCCACAGCACGGATCACCATGCCAAAAGCAATGGTAAGGCTGTCTGCAGGTCGAGTGAAGTTTTCCGTGCCGGAGCAGGCGTTATGCTTCCTTGCAGGAGCAAATTCTATCTTCACCGGGGAGAAACTGCTAACGACGCCAAATAACGACTTTGATGCTGATCAACACATGTTCAAGATGCTGGGTTTGATTCCAAAAGCTCCCAGTTTCACTGATGGTGCTGAGAAAGCCCCTGAAGAAGAGTCATTACAAGAAGCTGCTTCCAATTAA

Protein sequence

MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRDIYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQKLMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLGMIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIPKAPSFTDGAEKAPEEESLQEAASN
Homology
BLAST of CmoCh17G005740 vs. ExPASy Swiss-Prot
Match: P54967 (Biotin synthase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BIO2 PE=1 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 7.5e-174
Identity = 304/383 (79.37%), Postives = 341/383 (89.03%), Query Frame = 0

Query: 1   MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD 60
           M+  RS  RS L+PS         +S +SSLSA+SA   EA+R +R+GPRN+W+R EI+ 
Sbjct: 1   MMLVRSVFRSQLRPS--VSGGLQSASCYSSLSAASA---EAERTIREGPRNDWSRDEIKS 60

Query: 61  IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK 120
           +YDSP+LDLLFHGAQVHRH HNFREVQQCTLLSIKTGGCSEDCSYCPQSSRY TGVK+Q+
Sbjct: 61  VYDSPLLDLLFHGAQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYSTGVKAQR 120

Query: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLG 180
           LM+KD V++AAKKAKEAGSTRFCMGAAWRDTIGRKTNF+QIL+Y+K+IR MGMEVCCTLG
Sbjct: 121 LMSKDAVIDAAKKAKEAGSTRFCMGAAWRDTIGRKTNFSQILEYIKEIRGMGMEVCCTLG 180

Query: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240
           MIEKQQA++LKKAGLTAYNHNLDTSREYYPN+ITTRSYD+RL+TL  VRDAGINVCSGGI
Sbjct: 181 MIEKQQALELKKAGLTAYNHNLDTSREYYPNVITTRSYDDRLETLSHVRDAGINVCSGGI 240

Query: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300
           IGLGEAEEDR+GLLHTLATLP+HPESVPINAL+AVKGTPL+DQKPVEIWEMIRMI TARI
Sbjct: 241 IGLGEAEEDRIGLLHTLATLPSHPESVPINALLAVKGTPLEDQKPVEIWEMIRMIGTARI 300

Query: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIP 360
            MPKAMVRLSAGRV+FS+ EQALCFLAGANSIFTGEKLLTTPNNDFDADQ MFK LGLIP
Sbjct: 301 VMPKAMVRLSAGRVRFSMSEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKTLGLIP 360

Query: 361 KAPSFTDGAEKAPEEESLQEAAS 384
           K PSF   +E   E E+ ++ AS
Sbjct: 361 KPPSF---SEDDSESENCEKVAS 375

BLAST of CmoCh17G005740 vs. ExPASy Swiss-Prot
Match: Q11S94 (Biotin synthase OS=Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465) OX=269798 GN=bioB PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 9.0e-119
Identity = 209/319 (65.52%), Postives = 259/319 (81.19%), Query Frame = 0

Query: 50  RNNWTRQEIRDIYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQS 109
           RNNWT++EI  IY+SP+LDL++ GA VHR  H+ +EVQ CTLLSIKTGGC EDCSYCPQ+
Sbjct: 5   RNNWTKEEISAIYNSPILDLMYRGATVHREFHDPQEVQVCTLLSIKTGGCPEDCSYCPQA 64

Query: 110 SRYDTGVKSQKLMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIR 169
           +RY T VK +KLM    VL +A +AKE+GSTRFCMGAAWR+    K +F++++D VK + 
Sbjct: 65  ARYHTDVKVEKLMDVKDVLNSALEAKESGSTRFCMGAAWREVRDNK-DFDKVIDMVKGVS 124

Query: 170 EMGMEVCCTLGMIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVR 229
            MGMEVCCTLGM+  +QA  LK AGL AYNHNLDTS E+Y  +ITTR+YD+RL+TL+ VR
Sbjct: 125 TMGMEVCCTLGMLTPEQADKLKDAGLYAYNHNLDTSAEHYDKVITTRTYDDRLETLDNVR 184

Query: 230 DAGINVCSGGIIGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIW 289
           +A I+VCSGGIIG+GE+  DRVG+LHTLA +  HPESVP+NALV V+GTPL+DQ  V +W
Sbjct: 185 NAKISVCSGGIIGMGESHGDRVGMLHTLANMVEHPESVPVNALVPVEGTPLEDQPRVSVW 244

Query: 290 EMIRMIATARITMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDAD 349
           EM+RMIATARI MPKAMVRLSAGRV+ +  EQALCFLAGANSIF G+KLLTTPN + +AD
Sbjct: 245 EMVRMIATARIIMPKAMVRLSAGRVRMNTEEQALCFLAGANSIFAGDKLLTTPNPEVNAD 304

Query: 350 QHMFKMLGLIPKAPSFTDG 369
           + MF++L L P+  SF +G
Sbjct: 305 KEMFQVLNLKPR-QSFKNG 321

BLAST of CmoCh17G005740 vs. ExPASy Swiss-Prot
Match: Q5AYI7 (Biotin synthase, mitochondrial OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bioB PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-114
Identity = 206/380 (54.21%), Postives = 266/380 (70.00%), Query Frame = 0

Query: 3   FARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRDIY 62
           F RSF R+ ++     + SP  +S  S +  +   ++ A       PR NWTR E++ IY
Sbjct: 5   FTRSFPRAFIRSYGTVQSSPTAASFASRIPPALQEAVAA-----TAPRTNWTRDEVQQIY 64

Query: 63  DSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQKLM 122
           ++P+  L +  A VHR  H+   +Q CTL++IKTGGCSEDCSYC QSSRY TG+K+ K+ 
Sbjct: 65  ETPLNQLTYAAAAVHRRFHDPSAIQMCTLMNIKTGGCSEDCSYCAQSSRYSTGLKATKMS 124

Query: 123 TKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLGMI 182
             D VLE A+ AK  GSTRFCMGAAWRD  GRKT+   +   V  +REMGMEVC TLGMI
Sbjct: 125 PVDDVLEKARIAKANGSTRFCMGAAWRDMRGRKTSLKNVKQMVSGVREMGMEVCVTLGMI 184

Query: 183 EKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIG 242
           +  QA +LK AGLTAYNHNLDTSRE+YP IITTRSYDERLKTL  VRDAGINVCSGGI+G
Sbjct: 185 DADQAKELKDAGLTAYNHNLDTSREFYPTIITTRSYDERLKTLSHVRDAGINVCSGGILG 244

Query: 243 LGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITM 302
           LGEA+ DR+GL+HT+++LP+HPES P+NALV +KGTPL D+K +   +++R +ATARI +
Sbjct: 245 LGEADSDRIGLIHTVSSLPSHPESFPVNALVPIKGTPLGDRKMISFDKLLRTVATARIVL 304

Query: 303 PKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIPKA 362
           P  +VRL+AGR+  +  +Q  CF+AGAN++FTGEK+LTT  N +D D+ MF   G  P  
Sbjct: 305 PATIVRLAAGRISLTEEQQVACFMAGANAVFTGEKMLTTDCNGWDEDRAMFDRWGFYPMR 364

Query: 363 PSFTDGAEKAPEEESLQEAA 383
            SF      A  ++ +   A
Sbjct: 365 -SFEKETNAATPQQHVDSVA 378

BLAST of CmoCh17G005740 vs. ExPASy Swiss-Prot
Match: O59778 (Biotin synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bio2 PE=1 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 6.9e-111
Identity = 194/311 (62.38%), Postives = 242/311 (77.81%), Query Frame = 0

Query: 50  RNNWTRQEIRDIYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQS 109
           RNNWTR+EI+ IYD+P++DL+F  A +HR  H+ ++VQQCTLLSIKTGGC+EDC YC QS
Sbjct: 20  RNNWTREEIQKIYDTPLIDLIFRAASIHRKFHDPKKVQQCTLLSIKTGGCTEDCKYCAQS 79

Query: 110 SRYDTGVKSQKLMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIR 169
           SRY+TGVK+ KLM  D VLE AK AK  GSTRFCMG+AWRD  GR   F  IL+ +K++R
Sbjct: 80  SRYNTGVKATKLMKIDEVLEKAKIAKAKGSTRFCMGSAWRDLNGRNRTFKNILEIIKEVR 139

Query: 170 EMGMEVCCTLGMIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVR 229
            M MEVC TLGM+ +QQA +LK AGLTAYNHNLDTSREYY  II+TR+YDERL T++ +R
Sbjct: 140 SMDMEVCVTLGMLNEQQAKELKDAGLTAYNHNLDTSREYYSKIISTRTYDERLNTIDNLR 199

Query: 230 DAGINVCSGGIIGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQD--QKPVE 289
            AG+ VCSGGI+GLGE + DRVGL+H+LAT+PTHPESVP N LV + GTP+ D  ++ + 
Sbjct: 200 KAGLKVCSGGILGLGEKKHDRVGLIHSLATMPTHPESVPFNLLVPIPGTPVGDAVKERLP 259

Query: 290 IWEMIRMIATARITMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFD 349
           I   +R IATARI MPK ++R +AGR   S  EQAL F+AGAN++FTGEK+LTTP   +D
Sbjct: 260 IHPFLRSIATARICMPKTIIRFAAGRNTCSESEQALAFMAGANAVFTGEKMLTTPAVSWD 319

Query: 350 ADQHMFKMLGL 359
           +D  +F   GL
Sbjct: 320 SDSQLFYNWGL 330

BLAST of CmoCh17G005740 vs. ExPASy Swiss-Prot
Match: A5FLT1 (Biotin synthase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=bioB PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 2.4e-103
Identity = 181/314 (57.64%), Postives = 234/314 (74.52%), Query Frame = 0

Query: 50  RNNWTRQEIRDIYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQS 109
           ++NWT+ EI  IY+ P++DLL+  A +HR  H+   VQ  TLLSIKTGGC EDC YCPQ+
Sbjct: 6   KHNWTKDEIIAIYNKPLMDLLYEAATIHRQQHDPNVVQVSTLLSIKTGGCPEDCGYCPQA 65

Query: 110 SRYDTGVKSQKLMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIR 169
           +RY+TGV+   LMT   V   A +AK  GS+R CMGAAWR+ +     F+Q+L+ V+ I 
Sbjct: 66  ARYNTGVEGNDLMTVSQVKAQALRAKSNGSSRVCMGAAWRN-VKDGEEFDQVLEMVRTIN 125

Query: 170 EMGMEVCCTLGMIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVR 229
           ++ MEVCCTLGM+ + QA  L +AGL AYNHNLDTS EYY ++I+TR +++RL+T+E VR
Sbjct: 126 KLDMEVCCTLGMLTENQAQRLAEAGLYAYNHNLDTSEEYYKDVISTRGFEDRLQTIENVR 185

Query: 230 DAGINVCSGGIIGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIW 289
              + VCSGGIIG+GE+ EDR G+L  L+TL   PESVPINALVAV+GTP++++KPVEIW
Sbjct: 186 KTNVTVCSGGIIGMGESIEDRAGMLVALSTLNPQPESVPINALVAVEGTPMEEEKPVEIW 245

Query: 290 EMIRMIATARITMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDAD 349
           EMIRM+AT RI MP+  VRLSAGR   S   QA+CF AGANSIF G+KLLTTPN D + D
Sbjct: 246 EMIRMVATTRIVMPETQVRLSAGRTNMSREGQAMCFFAGANSIFAGDKLLTTPNPDVNED 305

Query: 350 QHMFKMLGLIPKAP 364
             MF+ LG++ + P
Sbjct: 306 MKMFETLGMVAQKP 318

BLAST of CmoCh17G005740 vs. ExPASy TrEMBL
Match: A0A6J1EPY7 (Biotin synthase OS=Cucurbita moschata OX=3662 GN=LOC111436330 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 4.7e-219
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD 60
           MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD
Sbjct: 1   MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD 60

Query: 61  IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK 120
           IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK
Sbjct: 61  IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK 120

Query: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLG 180
           LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLG
Sbjct: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLG 180

Query: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240
           MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI
Sbjct: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240

Query: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300
           IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI
Sbjct: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300

Query: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIP 360
           TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIP
Sbjct: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIP 360

Query: 361 KAPSFTDGAEKAPEEESLQEAASN 385
           KAPSFTDGAEKAPEEESLQEAASN
Sbjct: 361 KAPSFTDGAEKAPEEESLQEAASN 384

BLAST of CmoCh17G005740 vs. ExPASy TrEMBL
Match: A0A6J1JVA1 (Biotin synthase OS=Cucurbita maxima OX=3661 GN=LOC111488652 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 4.8e-216
Identity = 381/384 (99.22%), Postives = 382/384 (99.48%), Query Frame = 0

Query: 1   MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD 60
           MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD
Sbjct: 1   MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD 60

Query: 61  IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK 120
           IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK
Sbjct: 61  IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK 120

Query: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLG 180
           LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVK+IREMGMEVCCTLG
Sbjct: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLG 180

Query: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240
           MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI
Sbjct: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240

Query: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300
           IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI
Sbjct: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300

Query: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIP 360
           TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLT PNNDFDADQHMFKMLGLIP
Sbjct: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTMPNNDFDADQHMFKMLGLIP 360

Query: 361 KAPSFTDGAEKAPEEESLQEAASN 385
           KAPSFTDGAEKAPEEESLQ AASN
Sbjct: 361 KAPSFTDGAEKAPEEESLQ-AASN 383

BLAST of CmoCh17G005740 vs. ExPASy TrEMBL
Match: A0A1S3BDC5 (Biotin synthase OS=Cucumis melo OX=3656 GN=LOC103488656 PE=3 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 4.4e-209
Identity = 369/383 (96.34%), Postives = 376/383 (98.17%), Query Frame = 0

Query: 1   MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD 60
           MLFARSF  SHLKPSDI RHSP FSSLFSSLSASSAASIEA+RLLRDGPRN+WTRQ+IRD
Sbjct: 1   MLFARSFFWSHLKPSDICRHSPRFSSLFSSLSASSAASIEAERLLRDGPRNDWTRQQIRD 60

Query: 61  IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK 120
           IYDSP+LDLLFHGAQVHRH HNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVK+QK
Sbjct: 61  IYDSPLLDLLFHGAQVHRHAHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQK 120

Query: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLG 180
           LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVK+IREMGMEVCCTLG
Sbjct: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLG 180

Query: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240
           MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI
Sbjct: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240

Query: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300
           IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI
Sbjct: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300

Query: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIP 360
           TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQ MFKMLGLIP
Sbjct: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIP 360

Query: 361 KAPSFTDGAEKAPEEESLQEAAS 384
           KAPSFT+ AEKAPEEESLQEAAS
Sbjct: 361 KAPSFTEDAEKAPEEESLQEAAS 383

BLAST of CmoCh17G005740 vs. ExPASy TrEMBL
Match: A0A0A0KCA2 (Biotin synthase OS=Cucumis sativus OX=3659 GN=Csa_6G041720 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.7e-208
Identity = 367/382 (96.07%), Postives = 376/382 (98.43%), Query Frame = 0

Query: 1   MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD 60
           MLFARSF RS LKPSDI RHSP FSSLFSSLSASSAAS+EA+RLLRDGPRN+WTRQ+IRD
Sbjct: 1   MLFARSFFRSPLKPSDICRHSPRFSSLFSSLSASSAASVEAERLLRDGPRNDWTRQQIRD 60

Query: 61  IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK 120
           IYDSP+LDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTG+K+QK
Sbjct: 61  IYDSPLLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGLKAQK 120

Query: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLG 180
           LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVK+IREMGMEVCCTLG
Sbjct: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLG 180

Query: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240
           MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI
Sbjct: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240

Query: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300
           IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI
Sbjct: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300

Query: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIP 360
           TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQ MFKMLGLIP
Sbjct: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIP 360

Query: 361 KAPSFTDGAEKAPEEESLQEAA 383
           KAPSFT+ AEKAPEEESLQEAA
Sbjct: 361 KAPSFTEDAEKAPEEESLQEAA 382

BLAST of CmoCh17G005740 vs. ExPASy TrEMBL
Match: A0A6J1D9U9 (Biotin synthase OS=Momordica charantia OX=3673 GN=LOC111018547 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 7.7e-206
Identity = 362/384 (94.27%), Postives = 372/384 (96.88%), Query Frame = 0

Query: 1   MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD 60
           MLF RSF RS  +PSDI RHSP FS LFSS SASSAASIEA+R+L DGPRNNWTRQ+IRD
Sbjct: 1   MLFVRSFFRSQPRPSDICRHSPAFSRLFSSNSASSAASIEAERVLSDGPRNNWTRQQIRD 60

Query: 61  IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK 120
           +YDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVK+QK
Sbjct: 61  VYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQK 120

Query: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLG 180
           LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVK+IREMGMEVCCTLG
Sbjct: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLG 180

Query: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240
           MIEKQQA +LKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI
Sbjct: 181 MIEKQQAEELKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240

Query: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300
           IGLGEAEEDRVGLLHTLATLP+HPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI
Sbjct: 241 IGLGEAEEDRVGLLHTLATLPSHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300

Query: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIP 360
           TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQ MFKMLGLIP
Sbjct: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIP 360

Query: 361 KAPSFTDGAEKAPEEESLQEAASN 385
           KAPSF+DG EKAPEEESLQEAASN
Sbjct: 361 KAPSFSDGVEKAPEEESLQEAASN 384

BLAST of CmoCh17G005740 vs. TAIR 10
Match: AT2G43360.1 (Radical SAM superfamily protein )

HSP 1 Score: 611.3 bits (1575), Expect = 5.3e-175
Identity = 304/383 (79.37%), Postives = 341/383 (89.03%), Query Frame = 0

Query: 1   MLFARSFLRSHLKPSDIPRHSPPFSSLFSSLSASSAASIEADRLLRDGPRNNWTRQEIRD 60
           M+  RS  RS L+PS         +S +SSLSA+SA   EA+R +R+GPRN+W+R EI+ 
Sbjct: 1   MMLVRSVFRSQLRPS--VSGGLQSASCYSSLSAASA---EAERTIREGPRNDWSRDEIKS 60

Query: 61  IYDSPVLDLLFHGAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQK 120
           +YDSP+LDLLFHGAQVHRH HNFREVQQCTLLSIKTGGCSEDCSYCPQSSRY TGVK+Q+
Sbjct: 61  VYDSPLLDLLFHGAQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYSTGVKAQR 120

Query: 121 LMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLG 180
           LM+KD V++AAKKAKEAGSTRFCMGAAWRDTIGRKTNF+QIL+Y+K+IR MGMEVCCTLG
Sbjct: 121 LMSKDAVIDAAKKAKEAGSTRFCMGAAWRDTIGRKTNFSQILEYIKEIRGMGMEVCCTLG 180

Query: 181 MIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGI 240
           MIEKQQA++LKKAGLTAYNHNLDTSREYYPN+ITTRSYD+RL+TL  VRDAGINVCSGGI
Sbjct: 181 MIEKQQALELKKAGLTAYNHNLDTSREYYPNVITTRSYDDRLETLSHVRDAGINVCSGGI 240

Query: 241 IGLGEAEEDRVGLLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARI 300
           IGLGEAEEDR+GLLHTLATLP+HPESVPINAL+AVKGTPL+DQKPVEIWEMIRMI TARI
Sbjct: 241 IGLGEAEEDRIGLLHTLATLPSHPESVPINALLAVKGTPLEDQKPVEIWEMIRMIGTARI 300

Query: 301 TMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIP 360
            MPKAMVRLSAGRV+FS+ EQALCFLAGANSIFTGEKLLTTPNNDFDADQ MFK LGLIP
Sbjct: 301 VMPKAMVRLSAGRVRFSMSEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKTLGLIP 360

Query: 361 KAPSFTDGAEKAPEEESLQEAAS 384
           K PSF   +E   E E+ ++ AS
Sbjct: 361 KPPSF---SEDDSESENCEKVAS 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P549677.5e-17479.37Biotin synthase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BIO2 PE=1 SV=1[more]
Q11S949.0e-11965.52Biotin synthase OS=Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 10... [more]
Q5AYI71.8e-11454.21Biotin synthase, mitochondrial OS=Emericella nidulans (strain FGSC A4 / ATCC 381... [more]
O597786.9e-11162.38Biotin synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812... [more]
A5FLT12.4e-10357.64Biotin synthase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM... [more]
Match NameE-valueIdentityDescription
A0A6J1EPY74.7e-219100.00Biotin synthase OS=Cucurbita moschata OX=3662 GN=LOC111436330 PE=3 SV=1[more]
A0A6J1JVA14.8e-21699.22Biotin synthase OS=Cucurbita maxima OX=3661 GN=LOC111488652 PE=3 SV=1[more]
A0A1S3BDC54.4e-20996.34Biotin synthase OS=Cucumis melo OX=3656 GN=LOC103488656 PE=3 SV=1[more]
A0A0A0KCA21.7e-20896.07Biotin synthase OS=Cucumis sativus OX=3659 GN=Csa_6G041720 PE=3 SV=1[more]
A0A6J1D9U97.7e-20694.27Biotin synthase OS=Momordica charantia OX=3673 GN=LOC111018547 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G43360.15.3e-17579.37Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 89..297
e-value: 6.2E-42
score: 155.3
IPR010722Biotin and thiamin synthesis-associated domainSMARTSM00876BATS_2coord: 268..360
e-value: 1.4E-40
score: 150.8
IPR010722Biotin and thiamin synthesis-associated domainPFAMPF06968BATScoord: 268..358
e-value: 9.2E-18
score: 64.2
IPR024177Biotin synthasePIRSFPIRSF001619Biotin_synthcoord: 35..367
e-value: 2.6E-140
score: 465.2
IPR024177Biotin synthaseSFLDSFLDF00272biotin_synthasecoord: 49..363
e-value: 0.0
score: 502.1
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 96..254
e-value: 1.2E-13
score: 51.7
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 84..313
score: 32.566143
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 50..362
e-value: 3.2E-138
score: 462.2
IPR002684Biotin synthase/Biotin biosynthesis bifunctional protein BioABTIGRFAMTIGR00433TIGR00433coord: 61..358
e-value: 1.9E-114
score: 379.9
IPR002684Biotin synthase/Biotin biosynthesis bifunctional protein BioABPANTHERPTHR22976BIOTIN SYNTHASEcoord: 30..376
IPR002684Biotin synthase/Biotin biosynthesis bifunctional protein BioABHAMAPMF_01694BioBcoord: 51..361
score: 40.341496
NoneNo IPR availablePIRSRPIRSR001619-1PIRSR001619-1coord: 52..355
e-value: 2.3E-122
score: 405.6
NoneNo IPR availableSFLDSFLDG01278biotin_synthase_likecoord: 49..363
e-value: 0.0
score: 502.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 364..384
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 96..296
e-value: 1.44472E-15
score: 72.7513
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 51..361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh17G005740.1CmoCh17G005740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009102 biotin biosynthetic process
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0004076 biotin synthase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding