CmoCh17G005510 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh17G005510
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionrRNA N-glycosidase
LocationCmo_Chr17: 5594526 .. 5594855 (+)
RNA-Seq ExpressionCmoCh17G005510
SyntenyCmoCh17G005510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAACAATGCAAAGGTTTGGTTGGAGAAGTGTGTGGAGAAGAAGGCAGAGCAATATTGGGCGGTTTATAGTGATGGAAGTATAAGAGTAAATAGTAGCCGTAATTTCTGTGTGTCGGCAGCTTCAGATTCGCCAGGGGGGCTTATTACCATCACTAAGTGCAATGGATCATCAAAGCAACGATGGGTTTTCATGGCGGATGCCACAATTTTGAACCCCAAATCTGGGTTGGTCATGGAGGTTGAAGGCTCTCAAGTTTCCCGAAACAAAATTATTTTATACCCCATAAAGGGTCGTTCTAGCCAACAATGGACTTTGTTCTACTAA

mRNA sequence

ATGGATAACAATGCAAAGGTTTGGTTGGAGAAGTGTGTGGAGAAGAAGGCAGAGCAATATTGGGCGGTTTATAGTGATGGAAGTATAAGAGTAAATAGTAGCCGTAATTTCTGTGTGTCGGCAGCTTCAGATTCGCCAGGGGGGCTTATTACCATCACTAAGTGCAATGGATCATCAAAGCAACGATGGGTTTTCATGGCGGATGCCACAATTTTGAACCCCAAATCTGGGTTGGTCATGGAGGTTGAAGGCTCTCAAGTTTCCCGAAACAAAATTATTTTATACCCCATAAAGGGTCGTTCTAGCCAACAATGGACTTTGTTCTACTAA

Coding sequence (CDS)

ATGGATAACAATGCAAAGGTTTGGTTGGAGAAGTGTGTGGAGAAGAAGGCAGAGCAATATTGGGCGGTTTATAGTGATGGAAGTATAAGAGTAAATAGTAGCCGTAATTTCTGTGTGTCGGCAGCTTCAGATTCGCCAGGGGGGCTTATTACCATCACTAAGTGCAATGGATCATCAAAGCAACGATGGGTTTTCATGGCGGATGCCACAATTTTGAACCCCAAATCTGGGTTGGTCATGGAGGTTGAAGGCTCTCAAGTTTCCCGAAACAAAATTATTTTATACCCCATAAAGGGTCGTTCTAGCCAACAATGGACTTTGTTCTACTAA

Protein sequence

MDNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSKQRWVFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQWTLFY
Homology
[Close]
BLAST of CmoCh17G005510 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 7.4e-26
Identity = 56/102 (54.90%), Postives = 67/102 (65.69%), Query Frame = 0 

Query: 4   NAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSKQRW 63
           N  VWLE CV  + EQ WA+Y DG+IRVNS+R+ CV++    P  LI I KC GS  QRW
Sbjct: 462 NNFVWLEDCVLNRVEQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRW 521

Query: 64  VFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQW 106
           VF  + TI NP + LVM+V  S VS  KIILYP  G  +QQW
Sbjct: 522 VFNTNGTISNPNAKLVMDVAQSNVSLRKIILYPPTGNPNQQW 563

[Close]
BLAST of CmoCh17G005510 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 1.5e-23
Identity = 52/102 (50.98%), Postives = 65/102 (63.73%), Query Frame = 0 

Query: 4   NAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSKQRW 63
           N  VWLE CV  + +Q WA+Y DG+IRVNS+R+ CV++    P  LI I KC GS  QRW
Sbjct: 462 NNFVWLEDCVLNRVQQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRW 521

Query: 64  VFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQW 106
           VF  + TI NP + L+M+V    VS  KIILY   G  +QQW
Sbjct: 522 VFNTNGTISNPNAKLLMDVAQRDVSLRKIILYRPTGNPNQQW 563

[Close]
BLAST of CmoCh17G005510 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 7.6e-23
Identity = 54/111 (48.65%), Postives = 72/111 (64.86%), Query Frame = 0 

Query: 2   DNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGG--LITITKCNGSS 61
           D N  +WLE+CV  + EQ WA+YSDG+IRV+ +R  CV+A+S +     +ITI  C+GS+
Sbjct: 409 DGNTNMWLEECVPNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSN 468

Query: 62  KQRWVFMADATILNP-KSGLVMEVEGSQVSRNKIILYPIKGRSSQQWTLFY 110
            QRWVF+AD +I  P    L M+V  S V   KIIL+   G  +QQW LFY
Sbjct: 469 NQRWVFLADGSISTPGNQRLAMDVARSDVDLKKIILHRPHGDLNQQWVLFY 519

[Close]
BLAST of CmoCh17G005510 vs. ExPASy Swiss-Prot
Match: Q9M653 (Ribosome-inactivating protein PMRIPt OS=Polygonatum multiflorum OX=45371 GN=RIPt PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 2.2e-22
Identity = 50/104 (48.08%), Postives = 67/104 (64.42%), Query Frame = 0 

Query: 2   DNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSKQ 61
           +NN +VWLE C   + +Q+WA+YSDG+IRV+S R+ CV++   S    I I  C+G   Q
Sbjct: 487 ENNTRVWLEDCAVDRPQQWWALYSDGTIRVDSDRSLCVTSDGHSSRDAIIILTCDGGINQ 546

Query: 62  RWVFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQW 106
           R VF  D TILNP + LVM+V  S V+  +IILY   G  +QQW
Sbjct: 547 RLVFNTDGTILNPNAQLVMDVRQSNVALRQIILYQPTGNPNQQW 590

[Close]
BLAST of CmoCh17G005510 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 100.1 bits (248), Expect = 1.6e-20
Identity = 47/102 (46.08%), Postives = 61/102 (59.80%), Query Frame = 0 

Query: 4   NAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSKQRW 63
           N  VW+E C     +Q WA+Y D +IRVNS+R  CV+    +   LI I KC G   QRW
Sbjct: 455 NNGVWMEDCEATSLQQQWALYGDRTIRVNSTRGLCVTTNGYNSKDLIIILKCQGLPSQRW 514

Query: 64  VFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQW 106
            F +D  I+NPKS  VM+V  S VS  +II++P  G  +QQW
Sbjct: 515 FFNSDGAIVNPKSRHVMDVRASNVSLREIIIFPATGNPNQQW 556

[Close]
BLAST of CmoCh17G005510 vs. ExPASy TrEMBL
Match: A0A6J1JQR5 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488061 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 5.1e-54
Identity = 105/109 (96.33%), Postives = 108/109 (99.08%), Query Frame = 0 

Query: 1   MDNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSK 60
           MDNNAKVWLEKCVE+KAEQYWA+YSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSK
Sbjct: 430 MDNNAKVWLEKCVEQKAEQYWALYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSK 489

Query: 61  QRWVFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQWTLFY 110
           QRW FMADATILNPKSGLVMEVEGSQVSR+KIILYPIKGRSSQQWTLFY
Sbjct: 490 QRWAFMADATILNPKSGLVMEVEGSQVSRSKIILYPIKGRSSQQWTLFY 538

[Close]
BLAST of CmoCh17G005510 vs. ExPASy TrEMBL
Match: A0A0A0K979 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 9.3e-32
Identity = 66/108 (61.11%), Postives = 84/108 (77.78%), Query Frame = 0 

Query: 2   DNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSKQ 61
           +NN  +WLEKCV+ KAEQYWAVYSDGSIRVN  RN CVS++S+  G L+ I +C G+S Q
Sbjct: 433 NNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVSSSSNRSGALLIIDECKGTSNQ 492

Query: 62  RWVFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQWTLFY 110
           RW F+A+ TILNP++  V++V GS VS  +IILYP  G ++QQWTLFY
Sbjct: 493 RWNFLANGTILNPETKKVVDVYGSMVSAKRIILYPKTGLANQQWTLFY 540

[Close]
BLAST of CmoCh17G005510 vs. ExPASy TrEMBL
Match: A0A5D3D0Q4 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G00170 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.5e-29
Identity = 65/108 (60.19%), Postives = 80/108 (74.07%), Query Frame = 0 

Query: 2   DNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSKQ 61
           +NN  VWLEKCV+ KAEQYWAVY DGSIRVNS RN CVS++S+    LI I +CNG+S Q
Sbjct: 432 NNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLCVSSSSNRILALIIIEECNGTSNQ 491

Query: 62  RWVFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQWTLFY 110
           RW F+A+  ILNP++ +VM+V    VSR KIILY   G  +Q+W LFY
Sbjct: 492 RWNFLANGAILNPETKMVMDVYRFMVSRKKIILYRKTGERNQEWALFY 539

[Close]
BLAST of CmoCh17G005510 vs. ExPASy TrEMBL
Match: A0A1S3BDI7 (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103488711 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.5e-29
Identity = 65/108 (60.19%), Postives = 80/108 (74.07%), Query Frame = 0 

Query: 2   DNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGLITITKCNGSSKQ 61
           +NN  VWLEKCV+ KAEQYWAVY DGSIRVNS RN CVS++S+    LI I +CNG+S Q
Sbjct: 432 NNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLCVSSSSNRILALIIIEECNGTSNQ 491

Query: 62  RWVFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQWTLFY 110
           RW F+A+  ILNP++ +VM+V    VSR KIILY   G  +Q+W LFY
Sbjct: 492 RWNFLANGAILNPETKMVMDVYRFMVSRKKIILYRKTGERNQEWALFY 539

[Close]
BLAST of CmoCh17G005510 vs. ExPASy TrEMBL
Match: A0A1S3C7Q1 (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103497644 PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 8.5e-25
Identity = 58/110 (52.73%), Postives = 76/110 (69.09%), Query Frame = 0 

Query: 2   DNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCVSAASD--SPGGLITITKCNGSS 61
           +NN  + L+ C + K+EQYWA+Y DG+IRVNSSRN CVS  S   +PG +IT+  CNGSS
Sbjct: 429 NNNTNIELDDCAKNKSEQYWALYGDGTIRVNSSRNLCVSLTSSDCTPGRIITVLMCNGSS 488

Query: 62  KQRWVFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQWTLFY 110
            QRW F AD +I+NPK G+ ++V+        IILYP  G  +QQW+LFY
Sbjct: 489 SQRWNFQADGSIINPKCGMAIDVDTRWYD---IILYPKTGEYTQQWSLFY 535

The following BLAST results are available for this feature:
BLAST of CmoCh17G005510 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O224157.4e-2654.90Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=... [more]
Q413581.5e-2350.98Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1[more]
U3KRF87.6e-2348.65Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2[more]
Q9M6532.2e-2248.08Ribosome-inactivating protein PMRIPt OS=Polygonatum multiflorum OX=45371 GN=RIPt... [more]
P331831.6e-2046.08Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2[more]
BLAST of CmoCh17G005510 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JQR55.1e-5496.33rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488061 PE=3 SV=1[more]
A0A0A0K9799.3e-3261.11rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1[more]
A0A5D3D0Q41.5e-2960.19rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G... [more]
A0A1S3BDI71.5e-2960.19rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103488711 PE=3 SV=1[more]
A0A1S3C7Q18.5e-2552.73rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103497644 PE=3 SV=1[more]
BLAST of CmoCh17G005510 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 1..108
e-value: 5.7E-11
score: 52.5
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 4..105
e-value: 1.3E-23
score: 83.6
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 1..108
score: 29.924999
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 2..107
e-value: 1.90408E-25
score: 90.2574
NoneNo IPR availableGENE3D2.80.10.50coord: 1..109
e-value: 4.8E-37
score: 128.4
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 2..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh17G005510.1CmoCh17G005510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0035821 modulation of process of other organism
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0090729 toxin activity