CmoCh17G003110 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh17G003110
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein RALF-like 33
LocationCmo_Chr17: 1888832 .. 1889215 (+)
RNA-Seq ExpressionCmoCh17G003110
SyntenyCmoCh17G003110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTATCTCACGCGTTTCTGCTCTCATTGCTGCGTTCGCCGTTCTCGCCGCTCATTTTCTGATCTTATCGGCAGCGGCCATCGATTTCTCCGGCGACAACGAGATTCTCTTTGCTCCGACTAAATCCGAGTGCCGAGGCTCAATCGCCGAGTGCTTTCTCGCTGGAAACGATGACGATTCCGGCTTCGGAATGGAGTTCGAGATGGACTCTGAAATCAATCGCCGAATTCTAGCGACTACTCGCTACATCAGCTATGGTGCTCTGAGGAGGAACAACGTTCCTTGCTCCCGCCGCGGCGCTTCTTACTACAACTGCCGTCCAGGCGCTCAGGCGAATCCTTACACCCGTGGTTGTAGCGCCATTACTCGCTGCCGAAGTTAA

mRNA sequence

ATGGGTATCTCACGCGTTTCTGCTCTCATTGCTGCGTTCGCCGTTCTCGCCGCTCATTTTCTGATCTTATCGGCAGCGGCCATCGATTTCTCCGGCGACAACGAGATTCTCTTTGCTCCGACTAAATCCGAGTGCCGAGGCTCAATCGCCGAGTGCTTTCTCGCTGGAAACGATGACGATTCCGGCTTCGGAATGGAGTTCGAGATGGACTCTGAAATCAATCGCCGAATTCTAGCGACTACTCGCTACATCAGCTATGGTGCTCTGAGGAGGAACAACGTTCCTTGCTCCCGCCGCGGCGCTTCTTACTACAACTGCCGTCCAGGCGCTCAGGCGAATCCTTACACCCGTGGTTGTAGCGCCATTACTCGCTGCCGAAGTTAA

Coding sequence (CDS)

ATGGGTATCTCACGCGTTTCTGCTCTCATTGCTGCGTTCGCCGTTCTCGCCGCTCATTTTCTGATCTTATCGGCAGCGGCCATCGATTTCTCCGGCGACAACGAGATTCTCTTTGCTCCGACTAAATCCGAGTGCCGAGGCTCAATCGCCGAGTGCTTTCTCGCTGGAAACGATGACGATTCCGGCTTCGGAATGGAGTTCGAGATGGACTCTGAAATCAATCGCCGAATTCTAGCGACTACTCGCTACATCAGCTATGGTGCTCTGAGGAGGAACAACGTTCCTTGCTCCCGCCGCGGCGCTTCTTACTACAACTGCCGTCCAGGCGCTCAGGCGAATCCTTACACCCGTGGTTGTAGCGCCATTACTCGCTGCCGAAGTTAA

Protein sequence

MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDDSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCRS
Homology
BLAST of CmoCh17G003110 vs. ExPASy Swiss-Prot
Match: Q9LUS7 (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 2.9e-34
Identity = 83/138 (60.14%), Postives = 98/138 (71.01%), Query Frame = 0

Query: 2   GISRVSALIAAFA---VLAAH--FLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAG 61
           G+SR S   A FA   +LA H   + +S+ + +F+GD    F P ++ECRG+IAEC ++ 
Sbjct: 3   GLSRNSGAAAIFAILLILAVHNWSVAVSSQSTEFAGD----FPPFETECRGTIAECSVSA 62

Query: 62  NDDDSG--------FGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRP 121
              D G         G EFEMDSEINRRILAT RYISYGALRRN +PCSRRGASYYNCR 
Sbjct: 63  ALGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRR 122

Query: 122 GAQANPYTRGCSAITRCR 127
           GAQANPY+RGCSAITRCR
Sbjct: 123 GAQANPYSRGCSAITRCR 136

BLAST of CmoCh17G003110 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 1.9e-33
Identity = 76/113 (67.26%), Postives = 86/113 (76.11%), Query Frame = 0

Query: 14  AVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDDSGFGMEFEMDSEI 73
           A+L  HFL  +A     SGD    F P +S+C G+IAEC L+  ++      EFEMDSEI
Sbjct: 14  AILTVHFL-FAAVTSQSSGD----FVPIESKCNGTIAECSLSTAEE------EFEMDSEI 73

Query: 74  NRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 127
           NRRILATT+YISYGALRRN VPCSRRGASYYNCR GAQANPY+RGCSAITRCR
Sbjct: 74  NRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

BLAST of CmoCh17G003110 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 2.9e-29
Identity = 68/113 (60.18%), Postives = 80/113 (70.80%), Query Frame = 0

Query: 15  VLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDDSGFGMEFEMDSEIN 74
           VL   F I  AAA D    + ++ A +   C+GSI EC             EFE+DSE N
Sbjct: 11  VLIGAFFISMAAAGDSGAYDWVMPARSGGGCKGSIGECIAEEE--------EFELDSESN 70

Query: 75  RRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCRS 128
           RRILAT +YISYGAL++N+VPCSRRGASYYNC+PGAQANPY+RGCSAITRCRS
Sbjct: 71  RRILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

BLAST of CmoCh17G003110 vs. ExPASy Swiss-Prot
Match: Q9SRY3 (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 8.6e-26
Identity = 60/85 (70.59%), Postives = 66/85 (77.65%), Query Frame = 0

Query: 43  SECRGSIAECFLAGNDDDSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGAS 102
           S C GSIAEC  A          E EMDSEINRRILATT+YISY +L+RN+VPCSRRGAS
Sbjct: 45  SGCHGSIAECIGA---------EEEEMDSEINRRILATTKYISYQSLKRNSVPCSRRGAS 104

Query: 103 YYNCRPGAQANPYTRGCSAITRCRS 128
           YYNC+ GAQANPY+RGCS I RCRS
Sbjct: 105 YYNCQNGAQANPYSRGCSKIARCRS 120

BLAST of CmoCh17G003110 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 7.3e-25
Identity = 65/121 (53.72%), Postives = 81/121 (66.94%), Query Frame = 0

Query: 11  AAFAVLAAHFLILSA--AAIDFSGDNEILFAPTK---SECRGSIAECFLAGNDDDSGFGM 70
           A +AV+A   +++SA  +  DF    + + A +    S C GSIAEC             
Sbjct: 6   AIYAVIAILAIVISAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEE-------- 65

Query: 71  EFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRC 127
           E E DS+I+RRILA  +YISYGA+RRN+VPCSRRGASYYNC+ GAQANPY+RGCS ITRC
Sbjct: 66  EMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRC 118

BLAST of CmoCh17G003110 vs. ExPASy TrEMBL
Match: A0A6J1H3W7 (protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111460267 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.8e-63
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0

Query: 1   MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD 60
           MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD
Sbjct: 1   MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD 60

Query: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120
           SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS
Sbjct: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

BLAST of CmoCh17G003110 vs. ExPASy TrEMBL
Match: A0A6J1KVY0 (protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111499183 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 8.6e-61
Identity = 123/127 (96.85%), Postives = 123/127 (96.85%), Query Frame = 0

Query: 1   MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD 60
           MGISRVSALIAAFAVLAAHFLI SA AIDFSGDNEILFAPTKSECRGSI ECFLAGN DD
Sbjct: 1   MGISRVSALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDD 60

Query: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120
           SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS
Sbjct: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

BLAST of CmoCh17G003110 vs. ExPASy TrEMBL
Match: A0A6J1C9N4 (protein RALF-like 33 OS=Momordica charantia OX=3673 GN=LOC111009595 PE=3 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.4e-49
Identity = 102/127 (80.31%), Postives = 113/127 (88.98%), Query Frame = 0

Query: 1   MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD 60
           M ISRVS+L+AAFA+ AAH L+ SA A DFSGD+++L+ P KSECRGSIAEC LAG  DD
Sbjct: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAG--DD 60

Query: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120
           SGFG EFEMDSEINRRILAT RYISYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGCS
Sbjct: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 125

BLAST of CmoCh17G003110 vs. ExPASy TrEMBL
Match: A0A6J1KG89 (protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111494831 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.3e-48
Identity = 96/127 (75.59%), Postives = 115/127 (90.55%), Query Frame = 0

Query: 1   MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD 60
           M ISRV + +AA A++AAHFL+ SA  +DFSGD+++LF P +S+CRGSIAECFLAG+D+D
Sbjct: 1   MEISRVFSFLAASAIIAAHFLLSSAMVVDFSGDDKLLFVPAESKCRGSIAECFLAGDDND 60

Query: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120
           S FGMEFEMDSEINRRILA +RY+SYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGC+
Sbjct: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

BLAST of CmoCh17G003110 vs. ExPASy TrEMBL
Match: A0A6J1EV64 (protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111436882 PE=3 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.2e-48
Identity = 96/127 (75.59%), Postives = 116/127 (91.34%), Query Frame = 0

Query: 1   MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD 60
           M ISRV + +AA A++AAHFL+ SA A+DFSG +++LF P +S+CRGSIAECFLAG+D+D
Sbjct: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60

Query: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120
           S FGMEFEMDSEINRRILA++RY+SYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGC+
Sbjct: 61  SAFGMEFEMDSEINRRILASSRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

BLAST of CmoCh17G003110 vs. TAIR 10
Match: AT3G16570.1 (rapid alkalinization factor 23 )

HSP 1 Score: 146.0 bits (367), Expect = 2.1e-35
Identity = 83/138 (60.14%), Postives = 98/138 (71.01%), Query Frame = 0

Query: 2   GISRVSALIAAFA---VLAAH--FLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAG 61
           G+SR S   A FA   +LA H   + +S+ + +F+GD    F P ++ECRG+IAEC ++ 
Sbjct: 3   GLSRNSGAAAIFAILLILAVHNWSVAVSSQSTEFAGD----FPPFETECRGTIAECSVSA 62

Query: 62  NDDDSG--------FGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRP 121
              D G         G EFEMDSEINRRILAT RYISYGALRRN +PCSRRGASYYNCR 
Sbjct: 63  ALGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRR 122

Query: 122 GAQANPYTRGCSAITRCR 127
           GAQANPY+RGCSAITRCR
Sbjct: 123 GAQANPYSRGCSAITRCR 136

BLAST of CmoCh17G003110 vs. TAIR 10
Match: AT4G15800.1 (ralf-like 33 )

HSP 1 Score: 143.3 bits (360), Expect = 1.4e-34
Identity = 76/113 (67.26%), Postives = 86/113 (76.11%), Query Frame = 0

Query: 14  AVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDDSGFGMEFEMDSEI 73
           A+L  HFL  +A     SGD    F P +S+C G+IAEC L+  ++      EFEMDSEI
Sbjct: 14  AILTVHFL-FAAVTSQSSGD----FVPIESKCNGTIAECSLSTAEE------EFEMDSEI 73

Query: 74  NRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 127
           NRRILATT+YISYGALRRN VPCSRRGASYYNCR GAQANPY+RGCSAITRCR
Sbjct: 74  NRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

BLAST of CmoCh17G003110 vs. TAIR 10
Match: AT1G02900.1 (rapid alkalinization factor 1 )

HSP 1 Score: 117.9 bits (294), Expect = 6.1e-27
Identity = 60/85 (70.59%), Postives = 66/85 (77.65%), Query Frame = 0

Query: 43  SECRGSIAECFLAGNDDDSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGAS 102
           S C GSIAEC  A          E EMDSEINRRILATT+YISY +L+RN+VPCSRRGAS
Sbjct: 45  SGCHGSIAECIGA---------EEEEMDSEINRRILATTKYISYQSLKRNSVPCSRRGAS 104

Query: 103 YYNCRPGAQANPYTRGCSAITRCRS 128
           YYNC+ GAQANPY+RGCS I RCRS
Sbjct: 105 YYNCQNGAQANPYSRGCSKIARCRS 120

BLAST of CmoCh17G003110 vs. TAIR 10
Match: AT3G05490.1 (ralf-like 22 )

HSP 1 Score: 114.8 bits (286), Expect = 5.2e-26
Identity = 65/121 (53.72%), Postives = 81/121 (66.94%), Query Frame = 0

Query: 11  AAFAVLAAHFLILSA--AAIDFSGDNEILFAPTK---SECRGSIAECFLAGNDDDSGFGM 70
           A +AV+A   +++SA  +  DF    + + A +    S C GSIAEC             
Sbjct: 6   AIYAVIAILAIVISAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEE-------- 65

Query: 71  EFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRC 127
           E E DS+I+RRILA  +YISYGA+RRN+VPCSRRGASYYNC+ GAQANPY+RGCS ITRC
Sbjct: 66  EMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRC 118

BLAST of CmoCh17G003110 vs. TAIR 10
Match: AT2G33775.1 (ralf-like 19 )

HSP 1 Score: 88.6 bits (218), Expect = 4.0e-18
Identity = 42/62 (67.74%), Postives = 49/62 (79.03%), Query Frame = 0

Query: 65  MEFEMDSEINRRILATTR-YISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAIT 124
           +++ MDSE NRR LA  R YISYGALR+NNVPCSRRG SYY+C+   +ANPY RGCS IT
Sbjct: 44  LDYLMDSETNRRQLAARRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCSVIT 103

Query: 125 RC 126
            C
Sbjct: 104 HC 105

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LUS72.9e-3460.14Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV... [more]
Q8L9P81.9e-3367.26Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1[more]
Q945T02.9e-2960.18Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1[more]
Q9SRY38.6e-2670.59Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1[more]
Q9MA627.3e-2553.72Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H3W71.8e-63100.00protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111460267 PE=3 SV=1[more]
A0A6J1KVY08.6e-6196.85protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111499183 PE=3 SV=1[more]
A0A6J1C9N43.4e-4980.31protein RALF-like 33 OS=Momordica charantia OX=3673 GN=LOC111009595 PE=3 SV=1[more]
A0A6J1KG891.3e-4875.59protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111494831 PE=3 SV=1[more]
A0A6J1EV642.2e-4875.59protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111436882 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G16570.12.1e-3560.14rapid alkalinization factor 23 [more]
AT4G15800.11.4e-3467.26ralf-like 33 [more]
AT1G02900.16.1e-2770.59rapid alkalinization factor 1 [more]
AT3G05490.15.2e-2653.72ralf-like 22 [more]
AT2G33775.14.0e-1867.74ralf-like 19 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 66..126
e-value: 5.7E-31
score: 107.3
NoneNo IPR availablePANTHERPTHR33136:SF50PROTEIN RALF-LIKE 33coord: 9..127
NoneNo IPR availablePANTHERPTHR33136RAPID ALKALINIZATION FACTOR-LIKEcoord: 9..127

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh17G003110.1CmoCh17G003110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019722 calcium-mediated signaling
cellular_component GO:0005576 extracellular region