CmoCh17G001080 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh17G001080
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectinesterase
LocationCmo_Chr17: 580652 .. 582249 (-)
RNA-Seq ExpressionCmoCh17G001080
SyntenyCmoCh17G001080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAATGATGATAATATTTGTACGAGGAAATGAAGATGAACGTTATAGAAGCGTGGGAGGGAAGAAATTGGCATGGCGGACAGTGGTTGTGGATCAGAGCGGCCATGGAAATTTCTCAACCATTCAAGCCGCCATTGATTCTGTTCCTTCAAATAATATGTTCTGGATCTCCATTCATATCAACCCTGGTATTTACAGGTCATTTTCAATTTCCTTAATTACCCTCAATTTCTCTTCTTATTTATTTATTTATTTGTTTGTTTGTTTGTTTGTTTGTTCTTGTTTAGAGAGAAGGTGAAAATCCCATATGATAAGCCGTATATCATTTTGAAAGGACAAAGAAGAAAGACAACAAAGGTTGTCTGGAACGACCATTTCACTCTTGCTCAAAGTCCTACTTTCACTTCTTCAGCTGATAATGTTGTCGTTAAATCCATCACTTTTGTGGTAAGTAAGTGAACATCATTCATTTGTTCATATTAACTCTGTTTTTTTGTGATCTAATTCTTGTGGGTATTAGAATTCGTATAACTTTCCATGGACTAATGGAAATCGAAGAGCTCCAGCAGTTGCAGCTATGATCACCGGTGATAAGGCTTTGTTTTATAAATGTGGGTTCTATGGTGTCCAAGATACCTTGTGGGATGATCAAGGTCGACATTATTATCATCGTTGCGTCATTCAAGGTGCTGTCGATTTTATTTTCGGAAGCGCCCAATCCATTTTTCAGGTATCTTTTACTGTTTCGATATGACGTTTGAAGCAGCATAGGTAGCGGGAACTTACTAGGGTTTAGGGTTTAGAGTGAACTTGTGTAACAGCCCAGATCGCTAGTAGATATTGTCTTCTTTGGGCTTTTCCTTTCGGGCTTCTCCTCAAAGCTTTAAAACGCGTCTGCTAGTGGAAGGTTTCCATACCCTTATAAATGGATGTGGGACATCACAACTTGTGCTTAGTTAAAGTTGAAGTTGTGAAACCCTGATCGTTTTTGGTGATATGATGTTTGAAGCAAAATAGGTGGCAAGAACTTACTGGGGTTTAGGGTGAGACTGTGCTCGGATAAAGATGAAGTCATGAAACCCTAATCGTTTTCGATGATGTCAAAGGATATGATGTTTGAATCAACATAGAAGGTATGAACTTACTAGGGTTTAGGGTTTAGGGTGAGACTGTGTTCGGGTAAAGATGAAGTCGTGAAACCTTATTCGATGTCAAATGGTATGACGTTTGAAGTAACATAGGTAGCAGGAACTTACTAGAGTTTAGGGTTTAGGGTTTAGGATGAGCCTGTAGTCGGTTAAAGATGAAGTCGTGAAACCCGAATCGTTTTCGTTGATGTAGGGATGTTCGATATCAGTGGTGGGAGAAGCACTTCTTCCATTGGGGTCTACAAGCTTCATAACAGCACAAGGAAGAACCGACCCAAATGATGCAAATGGGTTTGTTTTCAAAGATTGCAAAGTGTTTGGGAGTGGCTCAGCTTTCTTAGGAAGGCCATGGAGAGGCTACTCAAGGGTTATCTTTTACAATTCTAGCTTCTCCAACGTCATAAACCCTAGTGGTTGGGATCCTTGGCATTTTGTTGGTCATGAGTAA

mRNA sequence

ATGGGAATGATGATAATATTTGTACGAGGAAATGAAGATGAACGTTATAGAAGCGTGGGAGGGAAGAAATTGGCATGGCGGACAGTGGTTGTGGATCAGAGCGGCCATGGAAATTTCTCAACCATTCAAGCCGCCATTGATTCTGTTCCTTCAAATAATATGTTCTGGATCTCCATTCATATCAACCCTGGTATTTACAGAGAGAAGGTGAAAATCCCATATGATAAGCCGTATATCATTTTGAAAGGACAAAGAAGAAAGACAACAAAGGTTGTCTGGAACGACCATTTCACTCTTGCTCAAAGTCCTACTTTCACTTCTTCAGCTGATAATGTTGTCGTTAAATCCATCACTTTTGTGAATTCGTATAACTTTCCATGGACTAATGGAAATCGAAGAGCTCCAGCAGTTGCAGCTATGATCACCGGTGATAAGGCTTTGTTTTATAAATGTGGGTTCTATGGTGTCCAAGATACCTTGTGGGATGATCAAGGTCGACATTATTATCATCGTTGCGTCATTCAAGGTGCTGTCGATTTTATTTTCGGAAGCGCCCAATCCATTTTTCAGGGATGTTCGATATCAGTGGTGGGAGAAGCACTTCTTCCATTGGGGTCTACAAGCTTCATAACAGCACAAGGAAGAACCGACCCAAATGATGCAAATGGGTTTGTTTTCAAAGATTGCAAAGTGTTTGGGAGTGGCTCAGCTTTCTTAGGAAGGCCATGGAGAGGCTACTCAAGGGTTATCTTTTACAATTCTAGCTTCTCCAACGTCATAAACCCTAGTGGTTGGGATCCTTGGCATTTTGTTGGTCATGAGTAA

Coding sequence (CDS)

ATGGGAATGATGATAATATTTGTACGAGGAAATGAAGATGAACGTTATAGAAGCGTGGGAGGGAAGAAATTGGCATGGCGGACAGTGGTTGTGGATCAGAGCGGCCATGGAAATTTCTCAACCATTCAAGCCGCCATTGATTCTGTTCCTTCAAATAATATGTTCTGGATCTCCATTCATATCAACCCTGGTATTTACAGAGAGAAGGTGAAAATCCCATATGATAAGCCGTATATCATTTTGAAAGGACAAAGAAGAAAGACAACAAAGGTTGTCTGGAACGACCATTTCACTCTTGCTCAAAGTCCTACTTTCACTTCTTCAGCTGATAATGTTGTCGTTAAATCCATCACTTTTGTGAATTCGTATAACTTTCCATGGACTAATGGAAATCGAAGAGCTCCAGCAGTTGCAGCTATGATCACCGGTGATAAGGCTTTGTTTTATAAATGTGGGTTCTATGGTGTCCAAGATACCTTGTGGGATGATCAAGGTCGACATTATTATCATCGTTGCGTCATTCAAGGTGCTGTCGATTTTATTTTCGGAAGCGCCCAATCCATTTTTCAGGGATGTTCGATATCAGTGGTGGGAGAAGCACTTCTTCCATTGGGGTCTACAAGCTTCATAACAGCACAAGGAAGAACCGACCCAAATGATGCAAATGGGTTTGTTTTCAAAGATTGCAAAGTGTTTGGGAGTGGCTCAGCTTTCTTAGGAAGGCCATGGAGAGGCTACTCAAGGGTTATCTTTTACAATTCTAGCTTCTCCAACGTCATAAACCCTAGTGGTTGGGATCCTTGGCATTTTGTTGGTCATGAGTAA

Protein sequence

MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHINPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE
Homology
BLAST of CmoCh17G001080 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 5.8e-96
Identity = 162/249 (65.06%), Postives = 197/249 (79.12%), Query Frame = 0

Query: 29  VVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHINPGIYREKVKIPYDKPYIILKGQRRKT 88
           V VDQSGHGNF+TIQ AIDSVP NN  W  I++  G+YREK+KIPY+KP+I+L G  ++ 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 89  TKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSYNFP---WTNGNRRAPAVAAMITGDK 148
           T+V W+DH+++AQSPTF++ ADN VVKSITF NSYNFP     N N R PAVAA+I GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 149 ALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFGSAQSIFQGCSISVVGEALLPLG 208
           + FY  GF G+QDTLWD  GRHY+HRC IQGAVDFIFG+ QSI+Q C I V+G  L P G
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEP-G 213

Query: 209 STSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGW 268
              +ITAQGRT+P DANGF+F +C V+G+G AFLGRPWRGYSRVIFYNS+ ++V+ P GW
Sbjct: 214 LAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGW 273

Query: 269 DPWHFVGHE 275
           D W+FVGHE
Sbjct: 274 DAWNFVGHE 281

BLAST of CmoCh17G001080 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.5e-88
Identity = 154/249 (61.85%), Postives = 187/249 (75.10%), Query Frame = 0

Query: 29  VVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHINPGIYREKVKIPYDKPYIILKGQRRKT 88
           V+VDQSGHGNF+TIQ AIDSVP NN  W  I++  G+YREK+ IP  KP+I++ G  +++
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 89  TKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSYNFPWT---NGNRRAPAVAAMITGDK 148
           T+V W+DH +LAQSPTF + ADN VVK ITF NSYNFP     N N R PAVAA I GDK
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGGDK 148

Query: 149 ALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFGSAQSIFQGCSISVVGEALLPLG 208
           + FY  GF G+QDTLWD  GRHY+HRC IQGAVDFI GS QSI+Q C I V+G  L P G
Sbjct: 149 SAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLGP-G 208

Query: 209 STSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGW 268
            T +ITAQGRT+ NDANGFVF +C V G G A+LGR WR YSRVIFYNS+ ++V++P GW
Sbjct: 209 VTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDPLGW 268

Query: 269 DPWHFVGHE 275
             W++ G+E
Sbjct: 269 WEWNYQGYE 276

BLAST of CmoCh17G001080 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 4.5e-64
Identity = 124/248 (50.00%), Postives = 153/248 (61.69%), Query Frame = 0

Query: 27  RTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHINPGIYREKVKIPYDKPYIILKGQRR 86
           +T++V+ +    F T+Q+AIDS+P  N  WI I I+ GIY EKV IP  K YI ++G   
Sbjct: 39  KTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGGGI 98

Query: 87  KTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSYNFPWTNGNRRAPAVAAMITGDKA 146
           + T + + DH     S TFTS   N+++  ITF N YN   ++ +   PAVAAM+ GDK 
Sbjct: 99  EKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA-SSSSPTKPAVAAMMLGDKY 158

Query: 147 LFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFGSAQSIFQGCSISVVGEALLPLGS 206
                 F G QDTL+DD GRHYY RCVI G +DFIFG AQSIF+GC++ +      P   
Sbjct: 159 AIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIYPPNEV 218

Query: 207 TSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGWD 266
              ITAQGR  P D  GFVFKDC V GSG A LGR W+ YSRVIFY S FS+ I P GWD
Sbjct: 219 YGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNILPIGWD 278

Query: 267 PWHFVGHE 275
            W   G E
Sbjct: 279 AWKAKGQE 285

BLAST of CmoCh17G001080 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 231.9 bits (590), Expect = 8.8e-60
Identity = 119/274 (43.43%), Postives = 164/274 (59.85%), Query Frame = 0

Query: 1   MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIH 60
           M +M +FV G       S   +++A+ T+ VD +G GNF+T+Q+AIDS+   N  WI + 
Sbjct: 14  MVVMTLFVYG------YSAAAEQIAY-TITVDLNGGGNFTTVQSAIDSISPPNHNWIRVF 73

Query: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFV 120
              GIYREKV IP +K +I L+G+  + T + ++DH     S TFT+ AD++V+  ITF 
Sbjct: 74  TQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFK 133

Query: 121 NSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180
           N+YN    N     PAVAA + GD+ +     F G+QDTL+D +GRHYY RC+I G +DF
Sbjct: 134 NTYNIVPNNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDF 193

Query: 181 IFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240
           IFG  QS+F+ C++++      P      ITA  R  P+D  GFVF DC V G G   LG
Sbjct: 194 IFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLG 253

Query: 241 RPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE 275
           R W   +RVIF  S  S+V+ P GWD W   G+E
Sbjct: 254 RAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNE 280

BLAST of CmoCh17G001080 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.6e-53
Identity = 113/240 (47.08%), Postives = 143/240 (59.58%), Query Frame = 0

Query: 31  VDQSGHGNFSTIQAAIDSVPS--NNMFWISIHINPGIYREKVKIPYDKPYIILKGQRRKT 90
           VDQSG G+FS IQ AI+S+P   NN     I + PGIYREKV IP +KPYI L G +   
Sbjct: 53  VDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQASN 112

Query: 91  TKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSYNFPWTNGNRRAPAVAAMITGDKALF 150
           T ++W+D   + +SPT T  A + V + +T  N +    T G     AVA  +  DKA F
Sbjct: 113 TFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFG---TAGR----AVALRVAADKAAF 172

Query: 151 YKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFGSAQSIFQGCSISVVGEALLPLGSTS 210
           Y C     QDTL DD G HY+  C I+GA DFI GSA S+++ C +     +L P  +  
Sbjct: 173 YGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHL----HSLSP--NNG 232

Query: 211 FITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGWDPW 269
            ITAQ RT   + +GF F  CK+ GSGS FLGRPW  YSRV+F  S FSNV+ P GW+ W
Sbjct: 233 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 279

BLAST of CmoCh17G001080 vs. ExPASy TrEMBL
Match: A0A6J1H4P1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460019 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.2e-162
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0

Query: 1   MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIH 60
           MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIH
Sbjct: 1   MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIH 60

Query: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFV 120
           INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFV
Sbjct: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFV 120

Query: 121 NSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180
           NSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF
Sbjct: 121 NSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180

Query: 181 IFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240
           IFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG
Sbjct: 181 IFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240

Query: 241 RPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE 275
           RPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE
Sbjct: 241 RPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE 274

BLAST of CmoCh17G001080 vs. ExPASy TrEMBL
Match: A0A6J1KUQ1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111498876 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.3e-158
Identity = 265/274 (96.72%), Postives = 270/274 (98.54%), Query Frame = 0

Query: 1   MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIH 60
           M MMIIFVRGNE+ERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFW+SIH
Sbjct: 1   MAMMIIFVRGNENERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWVSIH 60

Query: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFV 120
           INPGIYREKVKIPYDKPYIILKGQRRKTTKVVW+DHFTLAQSPTFTSSADNVVVKSITFV
Sbjct: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWDDHFTLAQSPTFTSSADNVVVKSITFV 120

Query: 121 NSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180
           NSYNFPWTNGNRRAPAVAAMITGDKA FYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF
Sbjct: 121 NSYNFPWTNGNRRAPAVAAMITGDKASFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180

Query: 181 IFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240
           IFGSAQSIFQGC ISVVGEA LPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG
Sbjct: 181 IFGSAQSIFQGCLISVVGEAFLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240

Query: 241 RPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE 275
           RPWRGYSRVIFYNSSFSN+INP+GWDPWHFVGHE
Sbjct: 241 RPWRGYSRVIFYNSSFSNIINPNGWDPWHFVGHE 274

BLAST of CmoCh17G001080 vs. ExPASy TrEMBL
Match: A0A5D3CTI5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00040 PE=4 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.1e-140
Identity = 228/272 (83.82%), Postives = 255/272 (93.75%), Query Frame = 0

Query: 3   MMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHIN 62
           MMIIFVRGNE E YR +G KK AW+T++VD++GHGNFSTIQAAIDSVPS+N FW+SIHI 
Sbjct: 12  MMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIR 71

Query: 63  PGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNS 122
           PG+YREKVKIPYDKPYIILKG R++ TKVVW+DH T+AQSPTFTSSADN+VVKSI+FVNS
Sbjct: 72  PGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNS 131

Query: 123 YNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIF 182
           YN+PW NGN R PAVAAMITGDK+ FY+CGFYGVQDTLWD+QGRHYYHRC IQGAVDFIF
Sbjct: 132 YNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIF 191

Query: 183 GSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRP 242
           G+AQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFK+C VFGSGSA+LGRP
Sbjct: 192 GAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRP 251

Query: 243 WRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE 275
           WR YSRVIF+NS+FSN+INP+GWDPWHFVG+E
Sbjct: 252 WRAYSRVIFHNSNFSNIINPNGWDPWHFVGYE 283

BLAST of CmoCh17G001080 vs. ExPASy TrEMBL
Match: A0A1S3C544 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497005 PE=4 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.1e-140
Identity = 228/272 (83.82%), Postives = 255/272 (93.75%), Query Frame = 0

Query: 3   MMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHIN 62
           MMIIFVRGNE E YR +G KK AW+T++VD++GHGNFSTIQAAIDSVPS+N FW+SIHI 
Sbjct: 12  MMIIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDSVPSDNRFWVSIHIR 71

Query: 63  PGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNS 122
           PG+YREKVKIPYDKPYIILKG R++ TKVVW+DH T+AQSPTFTSSADN+VVKSI+FVNS
Sbjct: 72  PGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSSADNIVVKSISFVNS 131

Query: 123 YNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIF 182
           YN+PW NGN R PAVAAMITGDK+ FY+CGFYGVQDTLWD+QGRHYYHRC IQGAVDFIF
Sbjct: 132 YNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHYYHRCTIQGAVDFIF 191

Query: 183 GSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRP 242
           G+AQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFK+C VFGSGSA+LGRP
Sbjct: 192 GAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKECNVFGSGSAYLGRP 251

Query: 243 WRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE 275
           WR YSRVIF+NS+FSN+INP+GWDPWHFVG+E
Sbjct: 252 WRAYSRVIFHNSNFSNIINPNGWDPWHFVGYE 283

BLAST of CmoCh17G001080 vs. ExPASy TrEMBL
Match: A0A6J1CDJ5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009749 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.5e-126
Identity = 212/274 (77.37%), Postives = 237/274 (86.50%), Query Frame = 0

Query: 1   MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIH 60
           +G+ IIF RG E + YRSVG KK+AWRTVVVDQSGHGNFSTIQAAID VPSNN FW+SI 
Sbjct: 13  IGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSIL 72

Query: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFV 120
           +  G+YREKV IPYDKPYIILKGQ ++ T+VVW+DH  +AQSPTFTSSADN+VVK I+F 
Sbjct: 73  VKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFE 132

Query: 121 NSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180
           NSYN PW NGN R PAVAAM+TGDK+ FYKCGFYG+QDTLWD  GRHY+HRC IQGAVDF
Sbjct: 133 NSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDF 192

Query: 181 IFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240
           IFG+AQSI++GC+ISVVGEA LP   T FITAQGRTDPND NGFVFKDCKV GSGS FLG
Sbjct: 193 IFGTAQSIYEGCTISVVGEA-LPPRITGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLG 252

Query: 241 RPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE 275
           RPWRGYSRVIFYNS+FSN+INP+GWDPW F G E
Sbjct: 253 RPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQE 285

BLAST of CmoCh17G001080 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 4.2e-97
Identity = 162/249 (65.06%), Postives = 197/249 (79.12%), Query Frame = 0

Query: 29  VVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHINPGIYREKVKIPYDKPYIILKGQRRKT 88
           V VDQSGHGNF+TIQ AIDSVP NN  W  I++  G+YREK+KIPY+KP+I+L G  ++ 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 89  TKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSYNFP---WTNGNRRAPAVAAMITGDK 148
           T+V W+DH+++AQSPTF++ ADN VVKSITF NSYNFP     N N R PAVAA+I GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 149 ALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFGSAQSIFQGCSISVVGEALLPLG 208
           + FY  GF G+QDTLWD  GRHY+HRC IQGAVDFIFG+ QSI+Q C I V+G  L P G
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEP-G 213

Query: 209 STSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGW 268
              +ITAQGRT+P DANGF+F +C V+G+G AFLGRPWRGYSRVIFYNS+ ++V+ P GW
Sbjct: 214 LAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGW 273

Query: 269 DPWHFVGHE 275
           D W+FVGHE
Sbjct: 274 DAWNFVGHE 281

BLAST of CmoCh17G001080 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.1e-89
Identity = 154/249 (61.85%), Postives = 187/249 (75.10%), Query Frame = 0

Query: 29  VVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHINPGIYREKVKIPYDKPYIILKGQRRKT 88
           V+VDQSGHGNF+TIQ AIDSVP NN  W  I++  G+YREK+ IP  KP+I++ G  +++
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 89  TKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSYNFPWT---NGNRRAPAVAAMITGDK 148
           T+V W+DH +LAQSPTF + ADN VVK ITF NSYNFP     N N R PAVAA I GDK
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGGDK 148

Query: 149 ALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFGSAQSIFQGCSISVVGEALLPLG 208
           + FY  GF G+QDTLWD  GRHY+HRC IQGAVDFI GS QSI+Q C I V+G  L P G
Sbjct: 149 SAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLGP-G 208

Query: 209 STSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGW 268
            T +ITAQGRT+ NDANGFVF +C V G G A+LGR WR YSRVIFYNS+ ++V++P GW
Sbjct: 209 VTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDPLGW 268

Query: 269 DPWHFVGHE 275
             W++ G+E
Sbjct: 269 WEWNYQGYE 276

BLAST of CmoCh17G001080 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 3.2e-65
Identity = 124/248 (50.00%), Postives = 153/248 (61.69%), Query Frame = 0

Query: 27  RTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIHINPGIYREKVKIPYDKPYIILKGQRR 86
           +T++V+ +    F T+Q+AIDS+P  N  WI I I+ GIY EKV IP  K YI ++G   
Sbjct: 39  KTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGGGI 98

Query: 87  KTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSYNFPWTNGNRRAPAVAAMITGDKA 146
           + T + + DH     S TFTS   N+++  ITF N YN   ++ +   PAVAAM+ GDK 
Sbjct: 99  EKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA-SSSSPTKPAVAAMMLGDKY 158

Query: 147 LFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFGSAQSIFQGCSISVVGEALLPLGS 206
                 F G QDTL+DD GRHYY RCVI G +DFIFG AQSIF+GC++ +      P   
Sbjct: 159 AIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIYPPNEV 218

Query: 207 TSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGWD 266
              ITAQGR  P D  GFVFKDC V GSG A LGR W+ YSRVIFY S FS+ I P GWD
Sbjct: 219 YGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNILPIGWD 278

Query: 267 PWHFVGHE 275
            W   G E
Sbjct: 279 AWKAKGQE 285

BLAST of CmoCh17G001080 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 6.3e-61
Identity = 119/274 (43.43%), Postives = 164/274 (59.85%), Query Frame = 0

Query: 1   MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIH 60
           M +M +FV G       S   +++A+ T+ VD +G GNF+T+Q+AIDS+   N  WI + 
Sbjct: 14  MVVMTLFVYG------YSAAAEQIAY-TITVDLNGGGNFTTVQSAIDSISPPNHNWIRVF 73

Query: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFV 120
              GIYREKV IP +K +I L+G+  + T + ++DH     S TFT+ AD++V+  ITF 
Sbjct: 74  TQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFK 133

Query: 121 NSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180
           N+YN    N     PAVAA + GD+ +     F G+QDTL+D +GRHYY RC+I G +DF
Sbjct: 134 NTYNIVPNNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDF 193

Query: 181 IFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240
           IFG  QS+F+ C++++      P      ITA  R  P+D  GFVF DC V G G   LG
Sbjct: 194 IFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLG 253

Query: 241 RPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHE 275
           R W   +RVIF  S  S+V+ P GWD W   G+E
Sbjct: 254 RAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNE 280

BLAST of CmoCh17G001080 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 211.1 bits (536), Expect = 1.1e-54
Identity = 113/240 (47.08%), Postives = 143/240 (59.58%), Query Frame = 0

Query: 31  VDQSGHGNFSTIQAAIDSVPS--NNMFWISIHINPGIYREKVKIPYDKPYIILKGQRRKT 90
           VDQSG G+FS IQ AI+S+P   NN     I + PGIYREKV IP +KPYI L G +   
Sbjct: 53  VDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQASN 112

Query: 91  TKVVWNDHFTLAQSPTFTSSADNVVVKSITFVNSYNFPWTNGNRRAPAVAAMITGDKALF 150
           T ++W+D   + +SPT T  A + V + +T  N +    T G     AVA  +  DKA F
Sbjct: 113 TFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFG---TAGR----AVALRVAADKAAF 172

Query: 151 YKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDFIFGSAQSIFQGCSISVVGEALLPLGSTS 210
           Y C     QDTL DD G HY+  C I+GA DFI GSA S+++ C +     +L P  +  
Sbjct: 173 YGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHL----HSLSP--NNG 232

Query: 211 FITAQGRTDPNDANGFVFKDCKVFGSGSAFLGRPWRGYSRVIFYNSSFSNVINPSGWDPW 269
            ITAQ RT   + +GF F  CK+ GSGS FLGRPW  YSRV+F  S FSNV+ P GW+ W
Sbjct: 233 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4PSN05.8e-9665.06Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1[more]
Q3E9D31.5e-8861.85Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1[more]
O644794.5e-6450.00Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1[more]
Q4PSQ58.8e-6043.43Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2[more]
Q9SIJ91.6e-5347.08Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H4P12.2e-162100.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460019 PE=4 SV=1[more]
A0A6J1KUQ14.3e-15896.72Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111498876 PE=4 SV=1[more]
A0A5D3CTI53.1e-14083.82Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00040... [more]
A0A1S3C5443.1e-14083.82Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497005 PE=4 SV=1[more]
A0A6J1CDJ52.5e-12677.37Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009749 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G24130.14.2e-9765.06Pectin lyase-like superfamily protein [more]
AT5G18990.11.1e-8961.85Pectin lyase-like superfamily protein [more]
AT2G19150.13.2e-6550.00Pectin lyase-like superfamily protein [more]
AT2G47280.16.3e-6143.43Pectin lyase-like superfamily protein [more]
AT2G21610.11.1e-5447.08pectinesterase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 10..274
e-value: 1.8E-88
score: 299.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 29..269
e-value: 5.1E-59
score: 199.4
NoneNo IPR availablePANTHERPTHR31321:SF76PECTINESTERASE 10-RELATEDcoord: 10..274
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 10..274
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 174..183
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 27..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh17G001080.1CmoCh17G001080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity