CmoCh17G000520 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh17G000520
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	aspartic proteinase CDR1-like
Location	Cmo_Chr17: 261625 .. 263317 (+)
RNA-Seq Expression	CmoCh17G000520
Synteny	CmoCh17G000520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACTCATTTTCTCACTGATTTTGATTGTCTCCTCCGCCGCTGCCGCTGCCGCAGACGGTGGCTATGGCTTCTCCGTCGAACTGGTCCACCGTGACTTCCCCAAGTTCCCACTTTTCAACTCATCAGAGACACACTACCAACGAATCGCCAACGCTATCCGTCGCTCCATCAGCCGTGGGACGGTGTCGCTGACAGACACGGGGAGAGCCCCAATATCCAACAGCGGAGGCGCATACGTTGTGAAAGTATCCCTCGGAACGCCGCCGTTTTCGATTGTAGCCGTTGCTGACACTGGAAGCGACATCATTTGGACTCAGTGCAAACCTTGCCCGAATTGCTACCAGCAAATCGACCCGATGTTTGATCCGAGTAAATCGTCGACTTACAAGACAGTTCCGTGTTCCTCGCCGACTTGCTCGTTTGCAGGGCCGAGAAGTTCTTGTTCCTCGGATTCCGTGTGCGAGTACTCCATTTCATACGGCGATGGATCCCACAGCAATGGGGATATTGCCGTTGATACCCTTACAATGGACTCCACCTCCGGCCGCCCCATGGCGTTTCCACGGACTGCCATTGGCTGTGGCCATGACAATGCTGGCTCTTTTGATTCTAAAGTTTCTGGGATTGTCGGGCTCGGTCATGGTTCAGCTTCCCTTATCCAGCAGATGGGGCCGGCCACCGGTGGGAAATTCTCTTACTGTTTGGCACCGGTTGGAAACTCTCACGACTCGAGCTATCTTAACTTTGGCTCTAATGCTATCGTCTCGGGCTCTGGAGCCGTCTCGACTCCGTTTTATACTAGTGGTAAATACAAAATGTTTTCTTATCGATAAAATGATTCGTGGGTAGGTGAATAACTCAATAAGGTAAAATCATGGGTACATAAGAGATCATAGAAGTTCGTTGTCGTAACAAACAGTATCGAAGTCATGCTCTTAACTTAGCCAGATACGGGTCGTGTGCTCTAGAGAAAAGGAGTTGGCCTGGATTTAGGGTTTAAAAGTGGATAATAATAGTGTGTGATCTAGAAAGGAGTCGACCTAGATTTAAGGTTTAAAAGTGGATAATACTAGTCGTGTGCTCTAGAGAAAAGGAGTCGATTTTGATTTAGGGTTTACAAGTGGATAATATTATAGTTTTACGTGAGTTTGTTCGTTATACTAAGATGAATTGTATAAGTAATTATTTTTTTTGTTCATGTTTGTAGAAGGCAAGTACGAAACTTTCTACGTGTTGAAAATAGAAGCAATGAGTGTAGGAAGCAACAAATTTGATTTTTCAAGCTCTTCACCATTTGGAACAAACGGGAACATCATTATCGACTCCGGCACGACACTTACATTCTTACCACCGGACACCTACACAAGCTTCTCCAAGGCGATTTCCGATGCGATGGACCTCAAGCCCACGACTAGTCCAATTCAAGGCGTGGATTATTGCTATACAACCACCACCGACGACTATAAGGTGCCACCTGTCACGGTGCATTTCGAAGGCGCCGACGTGTCTCTCAAGCGAGAAAACCTGTTCATTAGGGTGGATAACAACGTCGTTTGCTTGGCATTTATGGACAGTAACGGCGTCGGCCTACAAATCTATGGCAACATTGCACAGACTAACTTCTTGGTTGGCTATGATATCAAGAAATCGACCGTTTCTTTCAAGCCAGCAAATTGCGCTGGCTCGTAA

mRNA sequence

ATGGCACTCATTTTCTCACTGATTTTGATTGTCTCCTCCGCCGCTGCCGCTGCCGCAGACGGTGGCTATGGCTTCTCCGTCGAACTGGTCCACCGTGACTTCCCCAAGTTCCCACTTTTCAACTCATCAGAGACACACTACCAACGAATCGCCAACGCTATCCGTCGCTCCATCAGCCGTGGGACGGTGTCGCTGACAGACACGGGGAGAGCCCCAATATCCAACAGCGGAGGCGCATACGTTGTGAAAGTATCCCTCGGAACGCCGCCGTTTTCGATTGTAGCCGTTGCTGACACTGGAAGCGACATCATTTGGACTCAGTGCAAACCTTGCCCGAATTGCTACCAGCAAATCGACCCGATGTTTGATCCGAGTAAATCGTCGACTTACAAGACAGTTCCGTGTTCCTCGCCGACTTGCTCGTTTGCAGGGCCGAGAAGTTCTTGTTCCTCGGATTCCGTGTGCGAGTACTCCATTTCATACGGCGATGGATCCCACAGCAATGGGGATATTGCCGTTGATACCCTTACAATGGACTCCACCTCCGGCCGCCCCATGGCGTTTCCACGGACTGCCATTGGCTGTGGCCATGACAATGCTGGCTCTTTTGATTCTAAAGTTTCTGGGATTGTCGGGCTCGGTCATGGTTCAGCTTCCCTTATCCAGCAGATGGGGCCGGCCACCGGTGGGAAATTCTCTTACTGTTTGGCACCGGTTGGAAACTCTCACGACTCGAGCTATCTTAACTTTGGCTCTAATGCTATCGTCTCGGGCTCTGGAGCCGTCTCGACTCCGTTTTATACTAGTGAAGGCAAGTACGAAACTTTCTACGTGTTGAAAATAGAAGCAATGAGTGTAGGAAGCAACAAATTTGATTTTTCAAGCTCTTCACCATTTGGAACAAACGGGAACATCATTATCGACTCCGGCACGACACTTACATTCTTACCACCGGACACCTACACAAGCTTCTCCAAGGCGATTTCCGATGCGATGGACCTCAAGCCCACGACTAGTCCAATTCAAGGCGTGGATTATTGCTATACAACCACCACCGACGACTATAAGGTGCCACCTGTCACGGTGCATTTCGAAGGCGCCGACGTGTCTCTCAAGCGAGAAAACCTGTTCATTAGGGTGGATAACAACGTCGTTTGCTTGGCATTTATGGACAGTAACGGCGTCGGCCTACAAATCTATGGCAACATTGCACAGACTAACTTCTTGGTTGGCTATGATATCAAGAAATCGACCGTTTCTTTCAAGCCAGCAAATTGCGCTGGCTCGTAA

Coding sequence (CDS)

ATGGCACTCATTTTCTCACTGATTTTGATTGTCTCCTCCGCCGCTGCCGCTGCCGCAGACGGTGGCTATGGCTTCTCCGTCGAACTGGTCCACCGTGACTTCCCCAAGTTCCCACTTTTCAACTCATCAGAGACACACTACCAACGAATCGCCAACGCTATCCGTCGCTCCATCAGCCGTGGGACGGTGTCGCTGACAGACACGGGGAGAGCCCCAATATCCAACAGCGGAGGCGCATACGTTGTGAAAGTATCCCTCGGAACGCCGCCGTTTTCGATTGTAGCCGTTGCTGACACTGGAAGCGACATCATTTGGACTCAGTGCAAACCTTGCCCGAATTGCTACCAGCAAATCGACCCGATGTTTGATCCGAGTAAATCGTCGACTTACAAGACAGTTCCGTGTTCCTCGCCGACTTGCTCGTTTGCAGGGCCGAGAAGTTCTTGTTCCTCGGATTCCGTGTGCGAGTACTCCATTTCATACGGCGATGGATCCCACAGCAATGGGGATATTGCCGTTGATACCCTTACAATGGACTCCACCTCCGGCCGCCCCATGGCGTTTCCACGGACTGCCATTGGCTGTGGCCATGACAATGCTGGCTCTTTTGATTCTAAAGTTTCTGGGATTGTCGGGCTCGGTCATGGTTCAGCTTCCCTTATCCAGCAGATGGGGCCGGCCACCGGTGGGAAATTCTCTTACTGTTTGGCACCGGTTGGAAACTCTCACGACTCGAGCTATCTTAACTTTGGCTCTAATGCTATCGTCTCGGGCTCTGGAGCCGTCTCGACTCCGTTTTATACTAGTGAAGGCAAGTACGAAACTTTCTACGTGTTGAAAATAGAAGCAATGAGTGTAGGAAGCAACAAATTTGATTTTTCAAGCTCTTCACCATTTGGAACAAACGGGAACATCATTATCGACTCCGGCACGACACTTACATTCTTACCACCGGACACCTACACAAGCTTCTCCAAGGCGATTTCCGATGCGATGGACCTCAAGCCCACGACTAGTCCAATTCAAGGCGTGGATTATTGCTATACAACCACCACCGACGACTATAAGGTGCCACCTGTCACGGTGCATTTCGAAGGCGCCGACGTGTCTCTCAAGCGAGAAAACCTGTTCATTAGGGTGGATAACAACGTCGTTTGCTTGGCATTTATGGACAGTAACGGCGTCGGCCTACAAATCTATGGCAACATTGCACAGACTAACTTCTTGGTTGGCTATGATATCAAGAAATCGACCGTTTCTTTCAAGCCAGCAAATTGCGCTGGCTCGTAA

Protein sequence

MALIFSLILIVSSAAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISRGTVSLTDTGRAPISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDPMFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDSTSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPVGNSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSSPFGTNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTDDYKVPPVTVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVSFKPANCAGS

Homology

[Close]

BLAST of CmoCh17G000520 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 3.1e-112
Identity = 218/440 (49.55%), Postives = 294/440 (66.82%), Query Frame = 0


Query: 1   MALIFSLIL----IVSS--AAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAI 60
           MA +FS +L    ++SS   + A A    GF+ +L+HRD PK P +N  ET  QR+ NAI
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAI 60

Query: 61  RRSISRGTVSLTDTGRAP-----ISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCK 120
            RS++R     T+    P     ++++ G Y++ VS+GTPPF I+A+ADTGSD++WTQC 
Sbjct: 61  HRSVNR-VFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 120

Query: 121 PCPNCYQQIDPMFDPSKSSTYKTVPCSSPTCSFAGPRSSCS-SDSVCEYSISYGDGSHSN 180
           PC +CY Q+DP+FDP  SSTYK V CSS  C+    ++SCS +D+ C YS+SYGD S++ 
Sbjct: 121 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 180

Query: 181 GDIAVDTLTMDSTSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPAT 240
           G+IAVDTLT+ S+  RPM      IGCGH+NAG+F+ K SGIVGLG G  SLI+Q+G + 
Sbjct: 181 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 240

Query: 241 GGKFSYCLAPVGNSHD-SSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVG 300
            GKFSYCL P+ +  D +S +NFG+NAIVSGSG VSTP   ++   ETFY L ++++SVG
Sbjct: 241 DGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPL-IAKASQETFYYLTLKSISVG 300

Query: 301 SNKFDFSSSSPFGTNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYC 360
           S +  +S S    + GNIIIDSGTTLT LP + Y+    A++ ++D +    P  G+  C
Sbjct: 301 SKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLC 360

Query: 361 YTTTTDDYKVPPVTVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTN 420
           Y + T D KVP +T+HF+GADV L   N F++V  ++VC AF  S      IYGN+AQ N
Sbjct: 361 Y-SATGDLKVPVITMHFDGADVKLDSSNAFVQVSEDLVCFAFRGS--PSFSIYGNVAQMN 420

Query: 421 FLVGYDIKKSTVSFKPANCA 428
           FLVGYD    TVSFKP +CA
Sbjct: 421 FLVGYDTVSKTVSFKPTDCA 435

[Close]

BLAST of CmoCh17G000520 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.9e-85
Identity = 179/426 (42.02%), Postives = 261/426 (61.27%), Query Frame = 0


Query: 25  FSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISRG-----TVSLTDTGRAPISNSGGA 84
           FSVEL+HRD P  P++N   T   R+  A  RS+SR       +S TD  ++ +  + G 
Sbjct: 26  FSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRFNHQLSQTDL-QSGLIGADGE 85

Query: 85  YVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDPMFDPSKSSTYKTVPCSSPT 144
           + + +++GTPP  + A+ADTGSD+ W QCKPC  CY++  P+FD  KSSTYK+ PC S  
Sbjct: 86  FFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPIFDKKKSSTYKSEPCDSRN 145

Query: 145 C-SFAGPRSSC-SSDSVCEYSISYGDGSHSNGDIAVDTLTMDSTSGRPMAFPRTAIGCGH 204
           C + +     C  S+++C+Y  SYGD S S GD+A +T+++DS SG P++FP T  GCG+
Sbjct: 146 CQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVSFPGTVFGCGY 205

Query: 205 DNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLA-PVGNSHDSSYLNFGSNAIV 264
           +N G+FD   SGI+GLG G  SLI Q+G +   KFSYCL+     ++ +S +N G+N+I 
Sbjct: 206 NNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLSHKSATTNGTSVINLGTNSIP 265

Query: 265 SG----SGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSS--------PFGTNGN 324
           S     SG VSTP    E    T+Y L +EA+SVG  K  ++ SS           T+GN
Sbjct: 266 SSLSKDSGVVSTPLVDKEPL--TYYYLTLEAISVGKKKIPYTGSSYNPNDDGILSETSGN 325

Query: 325 IIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQG-VDYCYTTTTDDYKVPPVTVH 384
           IIIDSGTTLT L    +  FS A+ +++      S  QG + +C+ + + +  +P +TVH
Sbjct: 326 IIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLSHCFKSGSAEIGLPEITVH 385

Query: 385 FEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVSFKP 430
           F GADV L   N F+++  ++VCL+ + +  V   IYGN AQ +FLVGYD++  TVSF+ 
Sbjct: 386 FTGADVRLSPINAFVKLSEDMVCLSMVPTTEVA--IYGNFAQMDFLVGYDLETRTVSFQH 445

[Close]

BLAST of CmoCh17G000520 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.1e-61
Identity = 155/418 (37.08%), Postives = 219/418 (52.39%), Query Frame = 0


Query: 24  GFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISR-GTVSLTDTGRAPISNS----GG 83
           GF + L H D  K      + T +Q +  AI R   R   +     G + +  S     G
Sbjct: 40  GFQIMLEHVDSGK------NLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDG 99

Query: 84  AYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDPMFDPSKSSTYKTVPCSSP 143
            Y++ +S+GTP     A+ DTGSD+IWTQC+PC  C+ Q  P+F+P  SS++ T+PCSS 
Sbjct: 100 EYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQ 159

Query: 144 TCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDSTSGRPMAFPRTAIGCGHD 203
            C      S   S++ C+Y+  YGDGS + G +  +TLT  S S      P    GCG +
Sbjct: 160 LCQALS--SPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVS-----IPNITFGCGEN 219

Query: 204 NAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPVGNSHDSSYLNFGSNAIVSG 263
           N G      +G+VG+G G  SL  Q+      KFSYC+ P+G+S  S+ L  GS A    
Sbjct: 220 NQGFGQGNGAGLVGMGRGPLSLPSQLDVT---KFSYCMTPIGSSTPSNLL-LGSLANSVT 279

Query: 264 SGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFD-----FSSSSPFGTNGNIIIDSGTTL 323
           +G+ +T    S  +  TFY + +  +SVGS +       F+ +S  GT G IIIDSGTTL
Sbjct: 280 AGSPNTTLIQS-SQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGT-GGIIIDSGTTL 339

Query: 324 TFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTD--DYKVPPVTVHFEGADVSL 383
           T+   + Y S  +     ++L        G D C+ T +D  + ++P   +HF+G D+ L
Sbjct: 340 TYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLEL 399

Query: 384 KRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVSFKPANCAGS 430
             EN FI   N ++CLA M S+  G+ I+GNI Q N LV YD   S VSF  A C  S
Sbjct: 400 PSENYFISPSNGLICLA-MGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCGAS 437

[Close]

BLAST of CmoCh17G000520 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.8e-59
Identity = 142/396 (35.86%), Postives = 218/396 (55.05%), Query Frame = 0


Query: 45  THYQRIANAIRRSISR----GTVSLTDTG-RAPISNSGGAYVVKVSLGTPPFSIVAVADT 104
           T Y+ I  AI+R   R      +  + +G   P+    G Y++ V++GTP  S  A+ DT
Sbjct: 56  TKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDT 115

Query: 105 GSDIIWTQCKPCPNCYQQIDPMFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSI 164
           GSD+IWTQC+PC  C+ Q  P+F+P  SS++ T+PC S  C    P  +C+++  C+Y+ 
Sbjct: 116 GSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDL-PSETCNNNE-CQYTY 175

Query: 165 SYGDGSHSNGDIAVDTLTMDSTSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSAS 224
            YGDGS + G +A +T T +++S      P  A GCG DN G      +G++G+G G  S
Sbjct: 176 GYGDGSTTQGYMATETFTFETSS-----VPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLS 235

Query: 225 LIQQMGPATGGKFSYCLAPVGNSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVL 284
           L  Q+G    G+FSYC+   G+S  S+ L  GS A     G+ ST    S     T+Y +
Sbjct: 236 LPSQLGV---GQFSYCMTSYGSSSPST-LALGSAASGVPEGSPSTTLIHS-SLNPTYYYI 295

Query: 285 KIEAMSVGSNKFDFSSSS----PFGTNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLK 344
            ++ ++VG +     SS+      GT G +IIDSGTTLT+LP D Y + ++A +D ++L 
Sbjct: 296 TLQGITVGGDNLGIPSSTFQLQDDGT-GGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLP 355

Query: 345 PTTSPIQGVDYCYTTTTD--DYKVPPVTVHFEGADVSLKRENLFIRVDNNVVCLAFMDSN 404
                  G+  C+   +D    +VP +++ F+G  ++L  +N+ I     V+CLA   S+
Sbjct: 356 TVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILISPAEGVICLAMGSSS 415

Query: 405 GVGLQIYGNIAQTNFLVGYDIKKSTVSFKPANCAGS 430
            +G+ I+GNI Q    V YD++   VSF P  C  S
Sbjct: 416 QLGISIFGNIQQQETQVLYDLQNLAVSFVPTQCGAS 438

[Close]

BLAST of CmoCh17G000520 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 8.4e-57
Identity = 147/361 (40.72%), Postives = 183/361 (50.69%), Query Frame = 0


Query: 73  ISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDPMFDPSKSSTYKT 132
           +S   G Y  ++ +GTP   +  V DTGSDI+W QC PC  CY Q DP+FDP KS TY T
Sbjct: 135 LSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYAT 194

Query: 133 VPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDSTSGRPMAFPRTA 192
           +PCSSP C         +    C Y +SYGDGS + GD + +TLT      R       A
Sbjct: 195 IPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF-----RRNRVKGVA 254

Query: 193 IGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPVGNSHDSSYLNFGS 252
           +GCGHDN G F    +G++GLG G  S   Q G     KFSYCL     S   S + FG 
Sbjct: 255 LGCGHDNEGLFVG-AAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFG- 314

Query: 253 NAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSSPFGT----NGNIIID 312
           NA VS   A  TP   S  K +TFY + +  +SVG  +    ++S F      NG +IID
Sbjct: 315 NAAVSRI-ARFTPL-LSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIID 374

Query: 313 SGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCY-TTTTDDYKVPPVTVHFEGA 372
           SGT++T L    Y +   A                 D C+  +  ++ KVP V +HF GA
Sbjct: 375 SGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGA 434

Query: 373 DVSLKRENLFIRVD-NNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVSFKPANC 428
           DVSL   N  I VD N   C AF  + G GL I GNI Q  F V YD+  S V F P  C
Sbjct: 435 DVSLPATNYLIPVDTNGKFCFAFAGTMG-GLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 485

[Close]

BLAST of CmoCh17G000520 vs. ExPASy TrEMBL
Match: A0A6J1H4A6 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111460028 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.6e-244
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0


Query: 1   MALIFSLILIVSSAAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISR 60
           MALIFSLILIVSSAAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISR
Sbjct: 1   MALIFSLILIVSSAAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISR 60

Query: 61  GTVSLTDTGRAPISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDP 120
           GTVSLTDTGRAPISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDP
Sbjct: 61  GTVSLTDTGRAPISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDP 120

Query: 121 MFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDS 180
           MFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDS
Sbjct: 121 MFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDS 180

Query: 181 TSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPVG 240
           TSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPVG
Sbjct: 181 TSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPVG 240

Query: 241 NSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSSPFG 300
           NSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSSPFG
Sbjct: 241 NSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSSPFG 300

Query: 301 TNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTDDYKVPPV 360
           TNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTDDYKVPPV
Sbjct: 301 TNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTDDYKVPPV 360

Query: 361 TVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVS 420
           TVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVS
Sbjct: 361 TVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVS 420

Query: 421 FKPANCAGS 430
           FKPANCAGS
Sbjct: 421 FKPANCAGS 429

[Close]

BLAST of CmoCh17G000520 vs. ExPASy TrEMBL
Match: A0A6J1KWJ4 (aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111498869 PE=3 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.2e-234
Identity = 411/429 (95.80%), Postives = 419/429 (97.67%), Query Frame = 0


Query: 1   MALIFSLILIVSSAAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISR 60
           MALIFSLIL VSSAAAAAADGGYGFSVELVHRDFPKFPLFN+SETHYQRIA+A+RRSISR
Sbjct: 1   MALIFSLILFVSSAAAAAADGGYGFSVELVHRDFPKFPLFNASETHYQRIADALRRSISR 60

Query: 61  GTVSLTDTGRAPISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDP 120
           GTVSLTDTGRAPI NSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDP
Sbjct: 61  GTVSLTDTGRAPIYNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDP 120

Query: 121 MFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDS 180
           MFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDS
Sbjct: 121 MFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDS 180

Query: 181 TSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPVG 240
           TSGRP+AFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPAT GKFSYCLAPVG
Sbjct: 181 TSGRPVAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATDGKFSYCLAPVG 240

Query: 241 NSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSSPFG 300
           NSHDSSYLNFGSNAIVSGSG VSTP YTSEG YETFYVL IEA+SVGSNKFDFSSSSPFG
Sbjct: 241 NSHDSSYLNFGSNAIVSGSGVVSTPIYTSEGDYETFYVLNIEAISVGSNKFDFSSSSPFG 300

Query: 301 TNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTDDYKVPPV 360
           TNGNIIIDSGTTLTFLPPDTYTSFSKAIS+AMDLKPTTSPIQGV+YCYTTTTDDYKVPPV
Sbjct: 301 TNGNIIIDSGTTLTFLPPDTYTSFSKAISEAMDLKPTTSPIQGVEYCYTTTTDDYKVPPV 360

Query: 361 TVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVS 420
           TVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFL+GYDIKK TVS
Sbjct: 361 TVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLIGYDIKKLTVS 420

Query: 421 FKPANCAGS 430
           FKP NCA S
Sbjct: 421 FKPQNCAAS 429

[Close]

BLAST of CmoCh17G000520 vs. ExPASy TrEMBL
Match: A0A6J1H313 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111460027 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.2e-218
Identity = 380/429 (88.58%), Postives = 405/429 (94.41%), Query Frame = 0


Query: 1   MALIFSLILIVSSAAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISR 60
           MALIFSLI  +S+ ++AAA+GG GFSVE++HRDFPK PLFN+SETHY RIA+A+RRSISR
Sbjct: 1   MALIFSLIFFISAVSSAAANGGNGFSVEMIHRDFPKSPLFNASETHYHRIADALRRSISR 60

Query: 61  GTVSLTDTGRAPISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDP 120
             VSLTDTG+API +SGGAY VK+SLGTPPFSIVA+ADTGSDIIWTQCKPCPNCYQQIDP
Sbjct: 61  EMVSLTDTGKAPIYSSGGAYAVKISLGTPPFSIVAIADTGSDIIWTQCKPCPNCYQQIDP 120

Query: 121 MFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMDS 180
           MFDPSKSSTY TVPCSSPTCSFAG  SSCSS SVCEYSISYGDGSHSNGDIA DTLTMDS
Sbjct: 121 MFDPSKSSTYMTVPCSSPTCSFAGRGSSCSSKSVCEYSISYGDGSHSNGDIAADTLTMDS 180

Query: 181 TSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPVG 240
           TSGRP+AFPR AIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAP+G
Sbjct: 181 TSGRPVAFPRIAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPIG 240

Query: 241 NSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSSPFG 300
           NSHDS+YLNFGSNAIVSGSGAVSTP YTSEG +ETFYVLKIEAMSVGSNKFDF+SS PFG
Sbjct: 241 NSHDSTYLNFGSNAIVSGSGAVSTPIYTSEGDFETFYVLKIEAMSVGSNKFDFTSSLPFG 300

Query: 301 TNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTDDYKVPPV 360
           TNGNIIIDSGTTLTFLP DTYTSFSKAIS+ MDLKPTTSPIQ ++YC+ TTTD+YKVPPV
Sbjct: 301 TNGNIIIDSGTTLTFLPSDTYTSFSKAISEGMDLKPTTSPIQDLEYCFMTTTDNYKVPPV 360

Query: 361 TVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVS 420
           TVHFEGADV LKRENLF+RVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVS
Sbjct: 361 TVHFEGADVYLKRENLFVRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVS 420

Query: 421 FKPANCAGS 430
           FKPANCAGS
Sbjct: 421 FKPANCAGS 429

[Close]

BLAST of CmoCh17G000520 vs. ExPASy TrEMBL
Match: A0A6J1H6D4 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111460029 PE=3 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 5.8e-202
Identity = 359/431 (83.29%), Postives = 395/431 (91.65%), Query Frame = 0


Query: 1   MALIFSLILIVSSAA-AAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSIS 60
           MA+I SLIL++SSAA +AAADGGYGFSVEL+HRDF KFPLFN+SETHYQRIA+A+RRSIS
Sbjct: 1   MAIIVSLILLISSAASSAAADGGYGFSVELIHRDFLKFPLFNASETHYQRIADALRRSIS 60

Query: 61  RGTVSLTDTGRAPISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQID 120
           RGTVS  DTG+API  SGGAYVVK+SLGTPPFSIVA+ADTGSDIIWTQCKPCPNCYQQI 
Sbjct: 61  RGTVSPPDTGKAPIYTSGGAYVVKISLGTPPFSIVAIADTGSDIIWTQCKPCPNCYQQIA 120

Query: 121 PMFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMD 180
           PMFDPSKSSTYKTV CSSPTCS  GP +SCSS+SVCEYSISYGDGSHSNGDIAVDTLTMD
Sbjct: 121 PMFDPSKSSTYKTVSCSSPTCSITGPGNSCSSNSVCEYSISYGDGSHSNGDIAVDTLTMD 180

Query: 181 STSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPV 240
           STSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASL+QQMGPATGGKFSYCLAP+
Sbjct: 181 STSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLVQQMGPATGGKFSYCLAPI 240

Query: 241 GNSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSSPF 300
           GNS+ SSYLNFGSNAIVSGSGAVSTP YT +G Y+ FYVLKIEAMSVGSNK++FSSSSPF
Sbjct: 241 GNSNYSSYLNFGSNAIVSGSGAVSTPIYTGKGYYKVFYVLKIEAMSVGSNKYNFSSSSPF 300

Query: 301 GTNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTDDYKVPP 360
           GT GNIIIDSGTTLTFL PD + SFS+AIS+ MDLK TTSPIQ +++CY +TTDDYKVPP
Sbjct: 301 GTKGNIIIDSGTTLTFLQPDIFASFSEAISEVMDLKSTTSPIQTLEFCYESTTDDYKVPP 360

Query: 361 VTVHFEGADVSLKRENLFIRVDNNVVCLAFM-DSNGVGLQIYGNIAQTNFLVGYDIKKST 420
           V  HF+G  V+LKRENLFIRV ++VVCLAF+ +S    +QIYGNIAQTNFLVGY+IKKS+
Sbjct: 361 VIAHFKGGKVNLKRENLFIRVADDVVCLAFVGNSEKNSMQIYGNIAQTNFLVGYNIKKSS 420

Query: 421 VSFKPANCAGS 430
           VSFKPANCA S
Sbjct: 421 VSFKPANCAAS 431

[Close]

BLAST of CmoCh17G000520 vs. ExPASy TrEMBL
Match: A0A6J1KUP6 (aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111498870 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.2e-183
Identity = 329/429 (76.69%), Postives = 376/429 (87.65%), Query Frame = 0


Query: 1   MALIFSLILIVSSA-AAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSIS 60
           MALIFSLIL +S+A ++AAADGGYGFSVE++HRDFPK PLFN+SETHY RIA+A+RRSIS
Sbjct: 1   MALIFSLILFISAAVSSAAADGGYGFSVEMIHRDFPKSPLFNASETHYHRIADALRRSIS 60

Query: 61  RGTVSLTDTGRAPISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQID 120
           R  VSLTDTG+API +SGGAYVVK+SLGTPPFSIVAVADTGS+IIWT+CKPCPNC++QI+
Sbjct: 61  RERVSLTDTGKAPIYSSGGAYVVKISLGTPPFSIVAVADTGSNIIWTRCKPCPNCHKQIE 120

Query: 121 PMFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDTLTMD 180
           PMFDPSKSSTYK VPCSSP CS +G  SSCSS+S+CEYS SY DG+HS GDIAVDT+TM 
Sbjct: 121 PMFDPSKSSTYKLVPCSSPNCSISGLESSCSSESMCEYSTSYYDGTHSKGDIAVDTVTMG 180

Query: 181 STSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLAPV 240
           STSG P+AFPRT IGCGHDN  +F SK+SGIVGLGHG ASL+QQMGPATGGKFSYCL PV
Sbjct: 181 STSGHPVAFPRTVIGCGHDNVAAFGSKISGIVGLGHGPASLVQQMGPATGGKFSYCLVPV 240

Query: 241 GNSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKY-ETFYVLKIEAMSVGSNKFDFSSSSP 300
           G S++SSYLNFGSNAIVSG GAVSTPFYTS   Y + FYVLK+EAMSVGSNKF F+++  
Sbjct: 241 GKSNNSSYLNFGSNAIVSGFGAVSTPFYTSAIDYFKGFYVLKVEAMSVGSNKFKFTNTLL 300

Query: 301 FGTNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTDDYKVP 360
             TNGNIIIDSGTTLT++P +TY +FS AIS  +DL PTTSPIQ ++YCY TTT+DYKVP
Sbjct: 301 LETNGNIIIDSGTTLTYIPMETYANFSNAISKLIDLNPTTSPIQFLNYCYETTTNDYKVP 360

Query: 361 PVTVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKST 420
           PVTVHFEG DV+L+RENLFIRV  NVVCLAF+      + IYGNIAQTNFLVGYDIKKS+
Sbjct: 361 PVTVHFEGGDVNLERENLFIRVAKNVVCLAFVGRK--DMFIYGNIAQTNFLVGYDIKKSS 420

Query: 421 VSFKPANCA 428
           VSFKP+NCA
Sbjct: 421 VSFKPSNCA 427

[Close]

BLAST of CmoCh17G000520 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 406.8 bits (1044), Expect = 2.2e-113
Identity = 218/440 (49.55%), Postives = 294/440 (66.82%), Query Frame = 0


Query: 1   MALIFSLIL----IVSS--AAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAI 60
           MA +FS +L    ++SS   + A A    GF+ +L+HRD PK P +N  ET  QR+ NAI
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAI 60

Query: 61  RRSISRGTVSLTDTGRAP-----ISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCK 120
            RS++R     T+    P     ++++ G Y++ VS+GTPPF I+A+ADTGSD++WTQC 
Sbjct: 61  HRSVNR-VFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 120

Query: 121 PCPNCYQQIDPMFDPSKSSTYKTVPCSSPTCSFAGPRSSCS-SDSVCEYSISYGDGSHSN 180
           PC +CY Q+DP+FDP  SSTYK V CSS  C+    ++SCS +D+ C YS+SYGD S++ 
Sbjct: 121 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 180

Query: 181 GDIAVDTLTMDSTSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPAT 240
           G+IAVDTLT+ S+  RPM      IGCGH+NAG+F+ K SGIVGLG G  SLI+Q+G + 
Sbjct: 181 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 240

Query: 241 GGKFSYCLAPVGNSHD-SSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVG 300
            GKFSYCL P+ +  D +S +NFG+NAIVSGSG VSTP   ++   ETFY L ++++SVG
Sbjct: 241 DGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPL-IAKASQETFYYLTLKSISVG 300

Query: 301 SNKFDFSSSSPFGTNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYC 360
           S +  +S S    + GNIIIDSGTTLT LP + Y+    A++ ++D +    P  G+  C
Sbjct: 301 SKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLC 360

Query: 361 YTTTTDDYKVPPVTVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTN 420
           Y + T D KVP +T+HF+GADV L   N F++V  ++VC AF  S      IYGN+AQ N
Sbjct: 361 Y-SATGDLKVPVITMHFDGADVKLDSSNAFVQVSEDLVCFAFRGS--PSFSIYGNVAQMN 420

Query: 421 FLVGYDIKKSTVSFKPANCA 428
           FLVGYD    TVSFKP +CA
Sbjct: 421 FLVGYDTVSKTVSFKPTDCA 435

[Close]

BLAST of CmoCh17G000520 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 363.2 bits (931), Expect = 2.8e-100
Identity = 198/434 (45.62%), Postives = 276/434 (63.59%), Query Frame = 0


Query: 2   ALIFSLILIVSSAAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISRG 61
           +LIF+ +L +   +   A    GF+++L+HRD PK P +NS+ET  QR+ NAIRRS +R 
Sbjct: 3   SLIFATLLSLLLLSNVNAYPKDGFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRS-ARS 62

Query: 62  TVSLTDTGRAP------ISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCY 121
           T+  ++   +P      I+++ G Y++ +S+GTPP  I+A+ADTGSD+IWTQC PC +CY
Sbjct: 63  TLQFSNDDASPNSPQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCY 122

Query: 122 QQIDPMFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDIAVDT 181
           QQ  P+FDP +SSTY+ V CSS  C      S  + ++ C Y+I+YGD S++ GD+AVDT
Sbjct: 123 QQTSPLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAVDT 182

Query: 182 LTMDSTSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYC 241
           +TM S+  RP++     IGCGH+N G+FD   SGI+GLG GS SL+ Q+  +  GKFSYC
Sbjct: 183 VTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSINGKFSYC 242

Query: 242 LAP-VGNSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFS 301
           L P    +  +S +NFG+N IVSG G VST     +    T+Y L +EA+SVGS K  F+
Sbjct: 243 LVPFTSETGLTSKINFGTNGIVSGDGVVSTSMVKKDP--ATYYFLNLEAISVGSKKIQFT 302

Query: 302 SSSPFGT-NGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTTTD 361
           S+  FGT  GNI+IDSGTTLT LP + Y      ++  +  +    P   +  CY  ++ 
Sbjct: 303 STI-FGTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCYRDSS- 362

Query: 362 DYKVPPVTVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYD 421
            +KVP +TVHF+G DV L   N F+ V  +V C AF  +    L I+GN+AQ NFLVGYD
Sbjct: 363 SFKVPDITVHFKGGDVKLGNLNTFVAVSEDVSCFAFAANE--QLTIFGNLAQMNFLVGYD 422

Query: 422 IKKSTVSFKPANCA 428
               TVSFK  +C+
Sbjct: 423 TVSGTVSFKKTDCS 429

[Close]

BLAST of CmoCh17G000520 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.4e-86
Identity = 179/426 (42.02%), Postives = 261/426 (61.27%), Query Frame = 0


Query: 25  FSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISRG-----TVSLTDTGRAPISNSGGA 84
           FSVEL+HRD P  P++N   T   R+  A  RS+SR       +S TD  ++ +  + G 
Sbjct: 26  FSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRFNHQLSQTDL-QSGLIGADGE 85

Query: 85  YVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDPMFDPSKSSTYKTVPCSSPT 144
           + + +++GTPP  + A+ADTGSD+ W QCKPC  CY++  P+FD  KSSTYK+ PC S  
Sbjct: 86  FFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPIFDKKKSSTYKSEPCDSRN 145

Query: 145 C-SFAGPRSSC-SSDSVCEYSISYGDGSHSNGDIAVDTLTMDSTSGRPMAFPRTAIGCGH 204
           C + +     C  S+++C+Y  SYGD S S GD+A +T+++DS SG P++FP T  GCG+
Sbjct: 146 CQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVSFPGTVFGCGY 205

Query: 205 DNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLA-PVGNSHDSSYLNFGSNAIV 264
           +N G+FD   SGI+GLG G  SLI Q+G +   KFSYCL+     ++ +S +N G+N+I 
Sbjct: 206 NNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLSHKSATTNGTSVINLGTNSIP 265

Query: 265 SG----SGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFSSSS--------PFGTNGN 324
           S     SG VSTP    E    T+Y L +EA+SVG  K  ++ SS           T+GN
Sbjct: 266 SSLSKDSGVVSTPLVDKEPL--TYYYLTLEAISVGKKKIPYTGSSYNPNDDGILSETSGN 325

Query: 325 IIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQG-VDYCYTTTTDDYKVPPVTVH 384
           IIIDSGTTLT L    +  FS A+ +++      S  QG + +C+ + + +  +P +TVH
Sbjct: 326 IIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLSHCFKSGSAEIGLPEITVH 385

Query: 385 FEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQTNFLVGYDIKKSTVSFKP 430
           F GADV L   N F+++  ++VCL+ + +  V   IYGN AQ +FLVGYD++  TVSF+ 
Sbjct: 386 FTGADVRLSPINAFVKLSEDMVCLSMVPTTEVA--IYGNFAQMDFLVGYDLETRTVSFQH 445

[Close]

BLAST of CmoCh17G000520 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 313.2 bits (801), Expect = 3.3e-85
Identity = 178/443 (40.18%), Postives = 265/443 (59.82%), Query Frame = 0


Query: 3   LIFSLILIVSSAAAAAADGGYGFSVELVHRDFPKFPLFNSSETHYQRIANAIRRSISRGT 62
           L  SL+ I    A+ ++      +VEL+HRD P  PL+N   T   R+  A  RSISR  
Sbjct: 7   LYCSLLAISFFFASNSSANRENLTVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISRSR 66

Query: 63  VSLTDTG-RAPISNSGGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPCPNCYQQIDPM 122
              T T  ++ + ++GG Y + +S+GTPP  + A+ADTGSD+ W QCKPC  CY+Q  P+
Sbjct: 67  RFTTKTDLQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPCQQCYKQNSPL 126

Query: 123 FDPSKSSTYKTVPCSSPTC-SFAGPRSSC-SSDSVCEYSISYGDGSHSNGDIAVDTLTMD 182
           FD  KSSTYKT  C S TC + +     C  S  +C+Y  SYGD S + GD+A +T+++D
Sbjct: 127 FDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKGDVATETISID 186

Query: 183 STSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGKFSYCLA-P 242
           S+SG  ++FP T  GCG++N G+F+   SGI+GLG G  SL+ Q+G + G KFSYCL+  
Sbjct: 187 SSSGSSVSFPGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLGSSIGKKFSYCLSHT 246

Query: 243 VGNSHDSSYLNFGSNAIVSG----SGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKFDFS 302
              ++ +S +N G+N+I S     S  ++TP    +   ET+Y L +EA++VG  K  ++
Sbjct: 247 AATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDP--ETYYFLTLEAVTVGKTKLPYT 306

Query: 303 SSSPFGTN-------GNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQG-VDY 362
               +G N       GNIIIDSGTTLT L    Y  F  A+ +++      S  QG + +
Sbjct: 307 GGG-YGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQGLLTH 366

Query: 363 CYTTTTDDYKVPPVTVHFEGADVSLKRENLFIRVDNNVVCLAFMDSNGVGLQIYGNIAQT 422
           C+ +   +  +P +T+HF  ADV L   N F++++ + VCL+ + +  V   IYGN+ Q 
Sbjct: 367 CFKSGDKEIGLPAITMHFTNADVKLSPINAFVKLNEDTVCLSMIPTTEVA--IYGNMVQM 426

Query: 423 NFLVGYDIKKSTVSFKPANCAGS 430
           +FLVGYD++  TVSF+  +C+G+
Sbjct: 427 DFLVGYDLETKTVSFQRMDCSGN 444

[Close]

BLAST of CmoCh17G000520 vs. TAIR 10
Match: AT2G28010.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.6e-69
Identity = 152/378 (40.21%), Postives = 214/378 (56.61%), Query Frame = 0


Query: 55  RRSISRGTVSLTDTGRAPISNS---GGAYVVKVSLGTPPFSIVAVADTGSDIIWTQCKPC 114
           RRS +   VS T +G +P +N+      Y++K+ +GTPPF I A+ DTGS+I WTQC PC
Sbjct: 37  RRSNASSRVSNTQSGSSPYANTVFDNSVYLMKLQVGTPPFEIQAIIDTGSEITWTQCLPC 96

Query: 115 PNCYQQIDPMFDPSKSSTYKTVPCSSPTCSFAGPRSSCSSDSVCEYSISYGDGSHSNGDI 174
            +CY+Q  P+FDPSKSST+K   C   +               C Y + Y D +++ G +
Sbjct: 97  VHCYEQNAPIFDPSKSSTFKEKRCDGHS---------------CPYEVDYFDHTYTMGTL 156

Query: 175 AVDTLTMDSTSGRPMAFPRTAIGCGHDNAGSFDSKVSGIVGLGHGSASLIQQMGPATGGK 234
           A +T+T+ STSG P   P T IGCGH+N+  F    SG+VGL  G +SLI QMG    G 
Sbjct: 157 ATETITLHSTSGEPFVMPETIIGCGHNNSW-FKPSFSGMVGLNWGPSSLITQMGGEYPGL 216

Query: 235 FSYCLAPVGNSHDSSYLNFGSNAIVSGSGAVSTPFYTSEGKYETFYVLKIEAMSVGSNKF 294
            SYC +  G    +S +NFG+NAIV+G G VST  + +  K   FY L ++A+SVG+ + 
Sbjct: 217 MSYCFSGQG----TSKINFGANAIVAGDGVVSTTMFMTTAK-PGFYYLNLDAVSVGNTRI 276

Query: 295 DFSSSSPFGTNGNIIIDSGTTLTFLPPDTYTSFSKAISDAMDLKPTTSPIQGVDYCYTTT 354
           +   ++     GNI+IDSGTTLT+ P        +A+   +       P      CY + 
Sbjct: 277 ETMGTTFHALEGNIVIDSGTTLTYFPVSYCNLVRQAVEHVVTAVRAADPTGNDMLCYNSD 336

Query: 355 TDDYKVPPVTVHFE-GADVSLKRENLFIRVDN-NVVCLAFMDSNGVGLQIYGNIAQTNFL 414
           T D   P +T+HF  G D+ L + N+++  +N  V CLA + ++     I+GN AQ NFL
Sbjct: 337 TIDI-FPVITMHFSGGVDLVLDKYNMYMESNNGGVFCLAIICNSPTQEAIFGNRAQNNFL 392

Query: 415 VGYDIKKSTVSFKPANCA 428
           VGYD     VSF P NC+
Sbjct: 397 VGYDSSSLLVSFSPTNCS 392

The following BLAST results are available for this feature:

BLAST of CmoCh17G000520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6XBF8	3.1e-112	49.55	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q3EBM5	1.9e-85	42.02	Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561...	[more]
Q766C3	1.1e-61	37.08	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.8e-59	35.86	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	8.4e-57	40.72	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]

BLAST of CmoCh17G000520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H4A6	3.6e-244	100.00	aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111460028 PE=3...	[more]
A0A6J1KWJ4	1.2e-234	95.80	aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111498869 PE=3 S...	[more]
A0A6J1H313	1.2e-218	88.58	aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111460027 PE=3...	[more]
A0A6J1H6D4	5.8e-202	83.29	aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111460029 PE=3...	[more]
A0A6J1KUP6	1.2e-183	76.69	aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111498870 PE=3 S...	[more]

BLAST of CmoCh17G000520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G33340.1	2.2e-113	49.55	Eukaryotic aspartyl protease family protein	[more]
AT1G64830.1	2.8e-100	45.62	Eukaryotic aspartyl protease family protein	[more]
AT2G35615.1	1.4e-86	42.02	Eukaryotic aspartyl protease family protein	[more]
AT1G31450.1	3.3e-85	40.18	Eukaryotic aspartyl protease family protein	[more]
AT2G28010.1	2.6e-69	40.21	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 65..249 e-value: 2.4E-53 score: 183.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 250..429 e-value: 5.3E-46 score: 158.6
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 76..426
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 277..422 e-value: 2.3E-29 score: 102.3
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 80..252 e-value: 4.8E-56 score: 189.8
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 6..427
None	No IPR available	PANTHER	PTHR47967:SF66	ASPARTIC PROTEINASE CDR1-RELATED	coord: 6..427
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 305..316
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 95..106
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 80..422 score: 46.300232
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 79..426 e-value: 5.84764E-87 score: 264.125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh17G000520.1	CmoCh17G000520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity